##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431445_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4329276
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.015651346784082	31.0	31.0	34.0	26.0	34.0
2	29.94697288876939	31.0	30.0	34.0	25.0	34.0
3	30.06683450073407	31.0	31.0	34.0	25.0	34.0
4	33.309974231257144	37.0	35.0	37.0	26.0	37.0
5	33.42644266616404	37.0	35.0	37.0	27.0	37.0
6	33.42802237602777	37.0	35.0	37.0	27.0	37.0
7	33.25477054361976	37.0	35.0	37.0	26.0	37.0
8	33.17929995685191	37.0	35.0	37.0	26.0	37.0
9	34.46269953682787	39.0	35.0	39.0	23.0	39.0
10-11	34.4423409364522	39.0	35.0	39.0	24.0	39.0
12-13	34.42334284069669	39.0	35.0	39.0	24.0	39.0
14-15	35.6111985006269	40.0	36.0	41.0	24.0	41.0
16-17	35.63035690032236	40.0	36.0	41.0	25.0	41.0
18-19	35.62306688231473	40.0	36.0	41.0	25.0	41.0
20-21	35.7271995363659	40.0	36.0	41.0	25.0	41.0
22-23	35.701807184388336	40.0	36.0	41.0	25.0	41.0
24-25	35.75528437087402	40.0	36.0	41.0	26.0	41.0
26-27	35.767713123395225	40.0	36.0	41.0	27.0	41.0
28-29	35.664707909590426	40.0	35.0	41.0	26.5	41.0
30-31	35.64553796061975	40.0	35.0	41.0	27.0	41.0
32-33	35.58328459539193	40.0	35.0	41.0	26.0	41.0
34-35	35.474742543556935	40.0	35.0	41.0	25.5	41.0
36-37	35.34207567269908	40.0	35.0	41.0	25.0	41.0
38-39	35.226963238195026	40.0	35.0	41.0	25.0	41.0
40-41	35.12921583193125	40.0	35.0	41.0	25.0	41.0
42-43	34.908733007551376	39.5	34.5	41.0	24.0	41.0
44-45	34.7811098206721	39.0	34.0	41.0	24.0	41.0
46-47	34.63811339355587	39.0	34.0	41.0	23.5	41.0
48-49	34.50464962270828	39.0	34.0	41.0	23.0	41.0
50-51	34.3441686323533	39.0	33.5	41.0	23.0	41.0
52-53	34.242847302874665	39.0	33.0	41.0	22.5	41.0
54-55	34.1672763529052	39.0	33.0	41.0	22.0	41.0
56-57	34.009622509629786	38.0	33.0	40.5	21.5	41.0
58-59	33.79652348337228	38.0	33.0	40.0	20.5	41.0
60-61	33.57326086394122	38.0	33.0	40.0	19.5	41.0
62-63	33.28153494949271	37.0	32.5	40.0	17.5	41.0
64-65	33.116848637046935	37.0	32.5	40.0	15.5	41.0
66-67	32.85026988346319	36.5	32.0	40.0	13.0	41.0
68-69	32.56985301930392	36.0	32.0	39.5	9.0	41.0
70-71	32.257370862934124	35.5	32.0	39.0	7.5	41.0
72-73	31.937690274309148	35.0	32.0	39.0	3.5	41.0
74-75	31.603977778270547	35.0	31.0	38.0	2.0	40.0
76-77	31.280314306595375	35.0	31.0	37.0	2.0	39.5
78-79	30.950501538825428	35.0	31.0	37.0	2.0	39.0
80-81	30.656187200816024	35.0	31.0	36.0	2.0	39.0
82-83	30.361636333650246	35.0	31.0	36.0	2.0	38.0
84-85	30.079674176467382	35.0	30.0	35.5	2.0	37.0
86-87	29.826923485589738	35.0	30.0	35.0	2.0	37.0
88-89	29.562028269853897	35.0	30.0	35.0	2.0	36.5
90-91	29.343323687378675	34.5	30.0	35.0	2.0	36.0
92-93	29.150368791456124	34.0	30.0	35.0	2.0	36.0
94-95	28.921883012309678	34.0	29.5	35.0	2.0	36.0
96-97	28.827034127646286	34.0	30.0	35.0	2.0	35.0
98-99	28.672072189437678	34.0	29.5	35.0	2.0	35.0
100	28.537087032566184	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.42417159023266393
1101	2	0.4832883345894139
1101	3	0.48235614495079204
1101	4	0.5188907587205449
1101	5	0.4205650905125182
1101	6	0.44817940068573847
1101	7	0.4723767890504931
1101	8	0.5173112311583665
1101	9	0.6460734617864432
1101	10-11	0.6741364134407846
1101	12-13	0.6077343481804505
1101	14-15	0.7020073017573409
1101	16-17	0.7189175541700692
1101	18-19	0.6385888987343691
1101	20-21	0.5974526006474932
1101	22-23	0.5150823683571701
1101	24-25	0.5302475470593322
1101	26-27	0.6407685936922292
1101	28-29	0.752332721913227
1101	30-31	0.6763204020084785
1101	32-33	0.6999207709794817
1101	34-35	0.6809710615648683
1101	36-37	0.6784715246556985
1101	38-39	0.6299279891168368
1101	40-41	0.6351554304514551
1101	42-43	0.5887831092993565
1101	44-45	0.5324876254030926
1101	46-47	0.48887512852496684
1101	48-49	0.516398130666694
1101	50-51	0.5120560780380785
1101	52-53	0.504167037453648
1101	54-55	0.5665573371538315
1101	56-57	0.5478673384647692
1101	58-59	0.5714113704923491
1101	60-61	0.6243557167401192
1101	62-63	0.6629045386095882
1101	64-65	0.6143261194356455
1101	66-67	0.6270182037512839
1101	68-69	0.5660098543100247
1101	70-71	0.5969759504592957
1101	72-73	0.6121674195751723
1101	74-75	0.5612372134889121
1101	76-77	0.5299678840647566
1101	78-79	0.49966116316517883
1101	80-81	0.47851221880839034
1101	82-83	0.4486420850715618
1101	84-85	0.4472758628549798
1101	86-87	0.43480804389601246
1101	88-89	0.49125416914003495
1101	90-91	0.49064848042901943
1101	92-93	0.5145984441392208
1101	94-95	0.5286364230371134
1101	96-97	0.5483585314256167
1101	98-99	0.5480693769526752
1101	100	0.500497721734483
1102	1	0.44276559393407666
1102	2	0.4815642934246078
1102	3	0.5049636739356913
1102	4	0.451058654409195
1102	5	0.39864337105088055
1102	6	0.38969552399765206
1102	7	0.39486077434841604
1102	8	0.45693334451259915
1102	9	0.5888325518767559
1102	10-11	0.5509082408334436
1102	12-13	0.5688364356866629
1102	14-15	0.668237816894063
1102	16-17	0.5778492224885099
1102	18-19	0.5782619859311282
1102	20-21	0.5908563166993943
1102	22-23	0.5663136273872809
1102	24-25	0.5819057155196177
1102	26-27	0.6324300036522175
1102	28-29	0.6878899862386376
1102	30-31	0.718002771147269
1102	32-33	0.690379900860755
1102	34-35	0.7215508869241773
1102	36-37	0.6644354530342369
1102	38-39	0.5858747904522374
1102	40-41	0.6348309305780546
1102	42-43	0.7068921336989149
1102	44-45	0.5646166958750527
1102	46-47	0.5116351731501823
1102	48-49	0.5064840681984109
1102	50-51	0.5152000528305152
1102	52-53	0.5162811208881166
1102	54-55	0.5865054787569761
1102	56-57	0.6009161606848821
1102	58-59	0.6553119919337504
1102	60-61	0.6609523883805366
1102	62-63	0.604780529971741
1102	64-65	0.6127583448693485
1102	66-67	0.6304082302053331
1102	68-69	0.6523780464083515
1102	70-71	0.6377441774919177
1102	72-73	0.6333104793017625
1102	74-75	0.5913506057953306
1102	76-77	0.6417940254722403
1102	78-79	0.7035631674225158
1102	80-81	0.6387009899790854
1102	82-83	0.6347932534460554
1102	84-85	0.606100348942217
1102	86-87	0.6295917346737383
1102	88-89	0.6975641206141105
1102	90-91	0.6586158431736884
1102	92-93	0.6665026502650093
1102	94-95	0.6955909710809394
1102	96-97	0.636744767032539
1102	98-99	0.7259625728964352
1102	100	0.698691131496858
1103	1	0.15595211981501933
1103	2	0.21425399686671298
1103	3	0.2499046349361791
1103	4	0.1363183706223552
1103	5	0.12817128710940295
1103	6	0.22640474457208626
1103	7	0.09831232685744595
1103	8	0.23550239945421936
1103	9	0.23567098168805956
1103	10-11	0.24770509139901975
1103	12-13	0.19655767039614602
1103	14-15	0.33392963147044696
1103	16-17	0.19565475053271086
1103	18-19	0.13781094545903017
1103	20-21	0.18046595776312557
1103	22-23	0.17376160649747874
1103	24-25	0.2142291333634887
1103	26-27	0.22213242765572971
1103	28-29	0.31932976026907767
1103	30-31	0.24161614470509818
1103	32-33	0.36560391023488137
1103	34-35	0.33330608494075165
1103	36-37	0.2994904223445971
1103	38-39	0.3802724927475438
1103	40-41	0.34541297757468925
1103	42-43	0.326769429448845
1103	44-45	0.2863805328181641
1103	46-47	0.26329414840299137
1103	48-49	0.2032677106000662
1103	50-51	0.20637801743209394
1103	52-53	0.2651236486238986
1103	54-55	0.3876361003035811
1103	56-57	0.2499711185925122
1103	58-59	0.3105836511506368
1103	60-61	0.30720553227649106
1103	62-63	0.2624240116274663
1103	64-65	0.3117984520767436
1103	66-67	0.33195976186365783
1103	68-69	0.3061077033924775
1103	70-71	0.33415188269764684
1103	72-73	0.32415490043396034
1103	74-75	0.3080227430581566
1103	76-77	0.2923176823390712
1103	78-79	0.28158759261625477
1103	80-81	0.2879914821644114
1103	82-83	0.3582743688938237
1103	84-85	0.33042639321522316
1103	86-87	0.31506259146411963
1103	88-89	0.33497798926767786
1103	90-91	0.31471759409025424
1103	92-93	0.27427435835074476
1103	94-95	0.19785126394651797
1103	96-97	0.18685392103674658
1103	98-99	0.17277051397580578
1103	100	0.25075703494069757
1104	1	0.3658645566862262
1104	2	0.38435963362746506
1104	3	0.3780330554521214
1104	4	0.39241359487512284
1104	5	0.30535307723901894
1104	6	0.3618691840981967
1104	7	0.3365101952834024
1104	8	0.3310221265781408
1104	9	0.45117986088999373
1104	10-11	0.4620297599104788
1104	12-13	0.4775111563697365
1104	14-15	0.5754069845359027
1104	16-17	0.5346557436259758
1104	18-19	0.4658453121832551
1104	20-21	0.4329548769549447
1104	22-23	0.46363809052314764
1104	24-25	0.43727622149594225
1104	26-27	0.5535963034725029
1104	28-29	0.5825353430501323
1104	30-31	0.5226682954090691
1104	32-33	0.5581536418335489
1104	34-35	0.6198734537176946
1104	36-37	0.5333332410077603
1104	38-39	0.5355559630678641
1104	40-41	0.5107678139970844
1104	42-43	0.4947426500319949
1104	44-45	0.5504463649621201
1104	46-47	0.5291124491405768
1104	48-49	0.4711927216621561
1104	50-51	0.45589272523478286
1104	52-53	0.41475227312319163
1104	54-55	0.41580677822696543
1104	56-57	0.5118537726467309
1104	58-59	0.5025090866062243
1104	60-61	0.4877393760464557
1104	62-63	0.4876437511858427
1104	64-65	0.41903734506887247
1104	66-67	0.4660026079325448
1104	68-69	0.46381285221506374
1104	70-71	0.3506949117485334
1104	72-73	0.4003476728109945
1104	74-75	0.47341261637638965
1104	76-77	0.39723831805183707
1104	78-79	0.43438175386831546
1104	80-81	0.3992244513219667
1104	82-83	0.3916328986417099
1104	84-85	0.39404383106787577
1104	86-87	0.41153340916080694
1104	88-89	0.4120814350268205
1104	90-91	0.3121099185356506
1104	92-93	0.2723733468345202
1104	94-95	0.3201120440819949
1104	96-97	0.3454071810691488
1104	98-99	0.4164346579859739
1104	100	0.39663205906105503
1105	1	0.12233221436213881
1105	2	0.21484606959640118
1105	3	0.17155905882119526
1105	4	0.11300472845478282
1105	5	0.04926346870481524
1105	6	0.15167557113975505
1105	7	0.11041395209363003
1105	8	0.1566224125427098
1105	9	0.07826074671029914
1105	10-11	0.11702758213620967
1105	12-13	0.07334934198329535
1105	14-15	0.20711743223509416
1105	16-17	0.1612528723835993
1105	18-19	0.18660120578959294
1105	20-21	0.23725765702801027
1105	22-23	0.18362760170246872
1105	24-25	0.23121660796147836
1105	26-27	0.29654449889697787
1105	28-29	0.334301412125086
1105	30-31	0.3244302131096717
1105	32-33	0.3422718162487044
1105	34-35	0.3658685089807676
1105	36-37	0.2542980359954967
1105	38-39	0.2428114414613276
1105	40-41	0.2891093673533618
1105	42-43	0.2647087364969565
1105	44-45	0.12382320148008574
1105	46-47	0.2057657987213446
1105	48-49	0.20162511433077412
1105	50-51	0.236239787170625
1105	52-53	0.22411744173965076
1105	54-55	0.2773855999517707
1105	56-57	0.29344045105492
1105	58-59	0.26033969504381815
1105	60-61	0.21996464193934173
1105	62-63	0.20468385044467396
1105	64-65	0.20243852106194993
1105	66-67	0.28990898983956725
1105	68-69	0.22506051194378784
1105	70-71	0.23001258622858245
1105	72-73	0.20814108986278512
1105	74-75	0.18964952150593817
1105	76-77	0.2127208422745177
1105	78-79	0.23492536514961415
1105	80-81	0.26747817256011786
1105	82-83	0.2869382926976556
1105	84-85	0.22200904260114385
1105	86-87	0.18116235675698533
1105	88-89	0.2721313641668104
1105	90-91	0.23116187790136422
1105	92-93	0.24157477579238673
1105	94-95	0.21194030381229112
1105	96-97	0.25678235412060957
1105	98-99	0.2837196078685018
1105	100	0.28400705831300144
1106	1	0.2238375165994526
1106	2	0.27617097069357754
1106	3	0.27980624426086464
1106	4	0.3252429427080301
1106	5	0.3177894549251761
1106	6	0.33758603522922215
1106	7	0.39173897683927095
1106	8	0.41166161474714613
1106	9	0.4703519753289882
1106	10-11	0.4919185249306892
1106	12-13	0.4348673235850882
1106	14-15	0.5576924392798617
1106	16-17	0.425253754311143
1106	18-19	0.4708710756186747
1106	20-21	0.47616166750201927
1106	22-23	0.520991580423356
1106	24-25	0.4812146207540948
1106	26-27	0.46735077615636555
1106	28-29	0.5798668361688897
1106	30-31	0.5973501602018771
1106	32-33	0.5870712331775181
1106	34-35	0.4846123323844651
1106	36-37	0.5316205306581239
1106	38-39	0.5208774583553435
1106	40-41	0.559247273167486
1106	42-43	0.5042647104618823
1106	44-45	0.48378875704268154
1106	46-47	0.46528015516619803
1106	48-49	0.376355476048154
1106	50-51	0.43419905766237576
1106	52-53	0.47985340546825483
1106	54-55	0.47180603435137414
1106	56-57	0.4980236235103206
1106	58-59	0.5577465022546235
1106	60-61	0.562196530494397
1106	62-63	0.5684387774309911
1106	64-65	0.5881560027805008
1106	66-67	0.5523252946750219
1106	68-69	0.5634250495851489
1106	70-71	0.541124500400457
1106	72-73	0.5283946241601996
1106	74-75	0.5768191366241062
1106	76-77	0.5770340886702243
1106	78-79	0.5891367899827102
1106	80-81	0.5462286351991743
1106	82-83	0.45948226015180893
1106	84-85	0.47397002375952724
1106	86-87	0.5388762714137378
1106	88-89	0.5108566977097553
1106	90-91	0.4914540788385544
1106	92-93	0.5422141928899933
1106	94-95	0.5800160697985532
1106	96-97	0.5744433555779445
1106	98-99	0.5550402087470232
1106	100	0.5468768144345688
1107	1	0.12073287786360964
1107	2	0.1315304346111894
1107	3	0.178415511196917
1107	4	0.06654658274749181
1107	5	0.08327922280697919
1107	6	0.04807512933681579
1107	7	0.08804396466616282
1107	8	0.15315926064842245
1107	9	0.17418282267689733
1107	10-11	0.21644172152586094
1107	12-13	0.16324698720087127
1107	14-15	0.21042323730051748
1107	16-17	0.19779722589319704
1107	18-19	0.2578833045607922
1107	20-21	0.27107737067634474
1107	22-23	0.27670899316289876
1107	24-25	0.2846696770506867
1107	26-27	0.31702980245679413
1107	28-29	0.37906845077950635
1107	30-31	0.33459301400061037
1107	32-33	0.38082526739760425
1107	34-35	0.3246135327016262
1107	36-37	0.32234200731497253
1107	38-39	0.3589448860641937
1107	40-41	0.3308934825768901
1107	42-43	0.3472481802637759
1107	44-45	0.36790765995375097
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2216	98-99	-2.1248144396514483
2216	100	-2.158672060859608
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	139891.0
3	30247.0
4	20490.0
5	17925.0
6	12536.0
7	11789.0
8	13382.0
9	13601.0
10	13688.0
11	14326.0
12	15647.0
13	16972.0
14	17965.0
15	18676.0
16	18164.0
17	17548.0
18	16534.0
19	15428.0
20	14845.0
21	16079.0
22	19881.0
23	31029.0
24	47997.0
25	57894.0
26	62056.0
27	50025.0
28	51946.0
29	62509.0
30	72190.0
31	84210.0
32	104826.0
33	137026.0
34	187260.0
35	289135.0
36	454541.0
37	775496.0
38	982212.0
39	402151.0
40	1159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44189145389243	21.143756400669123	13.275319851528733	22.139032293909715
2	16.23384678501905	22.800378014279257	38.149596540675	22.816178660026697
3	17.781021149137562	28.231838217679655	29.701820053413986	24.2853205797688
4	12.43724124357397	19.87253972045412	37.152051871177704	30.538167164794206
5	12.25400826648654	39.98547866801084	32.97654502259746	14.783968042905155
6	28.279550467523755	39.81635404471115	18.148252018359052	13.755843469406049
7	25.49886574170328	35.381558946779954	21.581701248839174	17.537874062677588
8	23.89663316494692	37.416919461890366	20.423651838374916	18.262795534787795
9	24.431349747827376	20.124745349885043	20.85932964793777	34.584575254349815
10-11	22.405827007509448	29.911045573998425	27.67063958783229	20.012487830659843
12-13	23.270413228791433	28.191731912953205	27.184797719186637	21.353057139068724
14-15	21.39868790353319	29.977569618498713	24.982247427205213	23.641495050762888
16-17	20.435883810054662	32.6735105593781	25.519531751244756	21.371073879322484
18-19	20.369654059157263	31.288821570607023	27.496127588215398	20.84539678202032
20-21	22.459110989144005	30.5096330915243	26.524219248811974	20.507036670519717
22-23	21.87848592739269	30.77158696451131	26.59155793710945	20.75836917098655
24-25	21.42359835806174	30.46724092926129	26.98692316754562	21.122237545131355
26-27	21.248151998151997	30.25151305151305	27.06834141834142	21.43199353199353
28-29	21.18576228615152	30.420047720566696	27.116202444616327	21.277987548665457
30-31	21.642999561450065	31.075761724973866	26.444411052382407	20.836827661193666
32-33	20.952835906950614	30.591669407833948	27.221003840098163	21.234490845117275
34-35	21.219971259356242	29.920691808223538	27.21487408008342	21.644462852336797
36-37	21.778757223847894	30.531054966213155	26.584002804357382	21.106185005581573
38-39	21.524568169764212	30.44451672158292	26.938015475110276	21.09289963354259
40-41	21.205728494123157	30.135011894130457	27.213534869044448	21.445724742701938
42-43	21.073570197937457	30.319575603949072	27.121093805493306	21.485760392620172
44-45	21.536283784384686	30.38767158775535	26.90752870782644	21.16851592003353
46-47	21.273480688827316	30.83460546806377	26.703591202560307	21.188322640548606
48-49	21.070527530088114	30.203138375992523	27.120732213813532	21.605601880105834
50-51	21.088120937951018	30.005756398102513	27.147804068101056	21.758318595845413
52-53	21.14184163221408	30.134959892708434	27.008171141691488	21.715027333386
54-55	21.34742461900203	30.603178497125878	26.747651943257406	21.301744940614686
56-57	20.937342817098727	30.317920193386584	26.806978964127502	21.937758025387186
58-59	20.938306270870864	29.8229918726568	27.233396659830454	22.005305196641885
60-61	21.43304528764703	30.4329878856344	26.571264598677352	21.562702228041218
62-63	21.18869149228598	30.315282747341843	26.799021974392083	21.697003785980094
64-65	21.18393094511915	29.98094167153587	27.091724023946433	21.743403359398542
66-67	21.08397186550799	30.08467396889609	26.68430606625874	22.147048099337177
68-69	21.648635453886804	30.333462035587655	26.64111255110313	21.376789959422407
70-71	21.688903611109765	29.99779166686879	26.786549588051162	21.526755133970283
72-73	21.344274969356004	30.135824537441113	27.160183698814883	21.359716794388
74-75	21.346564257056407	30.130630694101452	26.927346724565627	21.595458324276514
76-77	21.475424017455598	30.149113377886287	26.587263959448904	21.78819864520921
78-79	21.665571369667937	30.247510172402443	26.715806810398888	21.37111164753073
80-81	21.279337211120737	30.11141343202665	26.97996651036786	21.629282846484756
82-83	21.21659155851258	30.12534759585572	27.046904608159267	21.61115623747243
84-85	21.362758488785218	29.926685427842354	26.85437544018426	21.85618064318817
86-87	21.76248720739275	30.028200617648622	26.683683822764255	21.52562835219438
88-89	21.415282901586	29.88628822336552	26.8406383548335	21.85779052021498
90-91	21.286121671576574	29.74780041580714	26.754767454873864	22.211310457742425
92-93	21.50656073399836	29.98377285227059	26.5840872316024	21.925579182128654
94-95	21.41583891290418	29.785102788376385	26.797855301204432	22.00120299751501
96-97	21.42097521403374	29.7944389109919	26.76022307924007	22.02436279573429
98-99	21.27026097449481	29.872641508344387	26.760863504248018	22.096234012912788
100	21.526479088745322	30.088340626524545	26.452801842023565	21.93237844270656
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37460.0
1	28318.5
2	17018.0
3	12474.0
4	8324.0
5	5670.5
6	4440.5
7	4132.0
8	4267.5
9	4194.5
10	3438.0
11	2738.5
12	2592.5
13	2592.5
14	2681.5
15	2759.0
16	2944.0
17	3434.0
18	4165.5
19	4966.5
20	6001.0
21	7820.5
22	10600.5
23	14632.0
24	19615.0
25	25820.0
26	34595.5
27	45447.5
28	58614.0
29	72002.0
30	82421.5
31	91408.0
32	99596.5
33	107288.0
34	114845.5
35	121564.5
36	126370.0
37	129920.5
38	132061.5
39	132904.5
40	134215.0
41	133387.0
42	130071.5
43	127541.5
44	126126.5
45	125305.0
46	125682.0
47	125603.0
48	126151.0
49	127759.0
50	128052.0
51	124621.5
52	119625.0
53	116984.5
54	115626.5
55	114029.5
56	106594.0
57	96227.5
58	89860.5
59	83873.5
60	74164.0
61	63144.5
62	53057.0
63	42371.5
64	32521.5
65	25317.0
66	19907.0
67	15258.5
68	11659.5
69	9135.0
70	7036.0
71	5411.5
72	4357.5
73	3502.0
74	2836.5
75	2273.5
76	1615.0
77	1223.0
78	1015.0
79	733.0
80	474.0
81	318.0
82	224.0
83	163.5
84	147.0
85	125.5
86	96.5
87	74.5
88	55.5
89	45.5
90	44.0
91	35.0
92	24.0
93	17.5
94	16.0
95	18.0
96	14.0
97	14.0
98	19.5
99	19.0
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014482791117960601
2	0.00783040859487822
3	0.010325052041034113
4	0.009193223070093013
5	0.010902515801718348
6	0.011826457818813122
7	0.010532938994880437
8	0.007945901347015066
9	0.006860269476928705
10-11	0.006040270936757093
12-13	0.006790973825646598
14-15	0.007530127439322418
16-17	0.004839146314533885
18-19	0.005901679634192877
20-21	0.005543652102568651
22-23	0.006074918762398147
24-25	0.005682243405132867
26-27	0.006375199917953949
28-29	0.01718532151796282
30-31	0.005462807176072859
32-33	0.005081681094021263
34-35	0.0049661883418844165
36-37	0.0068949173025697605
38-39	0.006005623111116039
40-41	0.0057746376068423455
42-43	0.004769850663251776
44-45	0.004307879654704389
46-47	0.006155763688893939
48-49	0.0036495709675243623
50-51	0.004157739076926488
52-53	0.0031875999589769744
54-55	0.00502393471795284
56-57	0.0028526709777801184
58-59	0.0028757695282074875
60-61	0.0036842187931654165
62-63	0.0033723883623959297
64-65	0.004561963709405452
66-67	0.0032684448854727673
68-69	0.003753514444447524
70-71	0.0050585825435938946
72-73	0.0028757695282074875
74-75	0.0028873188034211727
76-77	0.0027833753264980103
78-79	0.0037188666188064704
80-81	0.004042246324789642
82-83	0.001720842006839019
84-85	0.0028411217025664337
86-87	0.0026678825743611636
88-89	0.0019633767863263974
90-91	0.0024368970700874696
92-93	0.002563939097438001
94-95	0.002078869538463244
96-97	0.0012011246222232077
98-99	0.0018247854837621811
100	0.002864220252993803
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4329276.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.6506380361448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.40537238482888	47.86845329298768
2	14.717973722347722	18.44180888608596
3	4.484216563584231	8.428170864024024
4	1.7673435304629077	4.429007992502156
5	0.8466262459539122	2.6520837243579334
6	0.46872408339806065	1.7619517732677388
7	0.2897600242095083	1.2707555275866125
8	0.19441390687768648	0.9744124247189359
9	0.13879496382830828	0.7826033736042438
>10	0.6209166063432515	6.713435001798477
>50	0.03936082718229409	1.6984055876993736
>100	0.02450428823522814	2.797490437738269
>500	0.0015869014417607174	0.6616541635763193
>1k	3.321419778854558E-4	0.34674575086237747
>5k	3.6904664209486956E-5	0.14739642340116976
>10k+	3.6904664209486956E-5	1.0256247757886259
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44359	1.0246285984076782	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6375	0.1472532589744798	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	3.464782564105407E-5	0.0
12-13	0.0	0.0	0.0	6.929565128210814E-5	0.0
14-15	0.0	1.154927521368469E-5	0.0	1.7323912820527035E-4	0.0
16-17	0.0	2.309855042736938E-5	0.0	2.309855042736938E-4	0.0
18-19	0.0	2.309855042736938E-5	0.0	2.7718260512843255E-4	0.0
20-21	0.0	2.309855042736938E-5	0.0	4.2732318290633354E-4	0.0
22-23	0.0	2.309855042736938E-5	0.0	0.001154927521368469	0.0
24-25	0.0	2.309855042736938E-5	0.0	0.0022983057675232533	0.0
26-27	1.154927521368469E-5	2.309855042736938E-5	0.0	0.00371886661880647	0.0
28-29	2.309855042736938E-5	2.309855042736938E-5	0.0	0.008558012933340355	0.0
30-31	2.309855042736938E-5	2.309855042736938E-5	0.0	0.020026443220529253	0.0
32-33	2.309855042736938E-5	2.309855042736938E-5	0.0	0.03955626760687006	0.0
34-35	2.309855042736938E-5	2.309855042736938E-5	0.0	0.07005790344621132	0.0
36-37	2.309855042736938E-5	2.309855042736938E-5	0.0	0.11411838838641841	0.0
38-39	2.309855042736938E-5	2.309855042736938E-5	0.0	0.17699264264971787	0.0
40-41	2.309855042736938E-5	2.309855042736938E-5	0.0	0.2510696938702915	0.0
42-43	2.309855042736938E-5	2.309855042736938E-5	0.0	0.3254701247968482	0.0
44-45	2.309855042736938E-5	2.309855042736938E-5	0.0	0.41612038594905937	0.0
46-47	2.309855042736938E-5	2.309855042736938E-5	0.0	0.526496809166244	0.0
48-49	2.309855042736938E-5	2.309855042736938E-5	0.0	0.6456968786466837	0.0
50-51	2.309855042736938E-5	2.309855042736938E-5	0.0	0.7710180639903762	0.0
52-53	2.309855042736938E-5	2.309855042736938E-5	0.0	0.8937868595118446	0.0
54-55	2.309855042736938E-5	2.309855042736938E-5	0.0	1.0310615447017006	0.0
56-57	2.309855042736938E-5	2.309855042736938E-5	0.0	1.1983065990710688	0.0
58-59	2.309855042736938E-5	2.309855042736938E-5	0.0	1.3829910590131005	0.0
60-61	2.309855042736938E-5	2.309855042736938E-5	0.0	1.5799523985072792	0.0
62-63	3.464782564105407E-5	2.309855042736938E-5	0.0	1.780101337960435	0.0
64-65	4.619710085473876E-5	2.309855042736938E-5	0.0	2.003972488702499	0.0
66-67	4.619710085473876E-5	2.309855042736938E-5	0.0	2.266741598364253	0.0
68-69	4.619710085473876E-5	2.309855042736938E-5	0.0	2.5602548786448356	0.0
70-71	4.619710085473876E-5	2.309855042736938E-5	0.0	2.872755167376716	0.0
72-73	4.619710085473876E-5	2.309855042736938E-5	0.0	3.2136782224094746	0.0
74-75	4.619710085473876E-5	2.309855042736938E-5	0.0	3.5616578845977944	0.0
76-77	4.619710085473876E-5	2.309855042736938E-5	0.0	3.929791032033994	0.0
78-79	4.619710085473876E-5	2.309855042736938E-5	0.0	4.3485331034565595	1.154927521368469E-5
80-81	4.619710085473876E-5	2.309855042736938E-5	0.0	4.796194560014191	2.309855042736938E-5
82-83	4.619710085473876E-5	2.309855042736938E-5	0.0	5.266365553963295	2.309855042736938E-5
84-85	4.619710085473876E-5	2.309855042736938E-5	0.0	5.740636540613258	2.309855042736938E-5
86-87	4.619710085473876E-5	2.309855042736938E-5	0.0	6.240339031283753	2.309855042736938E-5
88	4.619710085473876E-5	2.309855042736938E-5	0.0	6.64476000144135	2.309855042736938E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6500	0.0	44.55169	1
GTATCAA	10300	0.0	32.31416	1
TACCTGG	3620	0.0	30.77524	2
TCAACGC	11645	0.0	27.045311	4
ATCAACG	11760	0.0	26.90106	3
GTACATG	20195	0.0	26.188189	1
TATCAAC	12655	0.0	25.630001	2
TACATGG	20305	0.0	24.932991	2
GTACCTG	4635	0.0	24.544968	1
CATGGGT	9740	0.0	24.516666	4
CAACGCA	13010	0.0	24.243866	5
ACATGGG	20770	0.0	23.28845	3
AACGCAG	13775	0.0	22.92895	6
TCTGTCG	1240	0.0	21.9838	8
GTGGTAT	3660	0.0	21.578688	1
CGCAGAG	14775	0.0	20.835806	8
ACGCAGA	15040	0.0	20.687912	7
GTCTCGC	1165	0.0	20.579796	1
ATGGGTG	5865	0.0	20.277256	5
TATAACG	330	3.274181E-11	19.942326	2
>>END_MODULE