FastQCFastQC Report
Wed 25 May 2016
SRR2431445_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431445_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4329276
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT403750.9326039735050387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351780.812560806934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259820.6001465372039112No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237310.5481517001919027No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124120.28669920790450876No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87420.2019275278360631No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80660.18631290774716142No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64170.14822339809242932No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136750.063.3794021
GTATCAA259300.043.133281
ATAACGC4250.040.9451833
TATAACG4800.039.1930272
GTGGTAT46100.036.1316831
TGGTATC47950.034.03542
TATCAAC346500.032.0330852
ATCAACG347850.031.8411663
TCAACGC351150.031.5285364
CAACGCA364650.030.4246125
AACGCAG377600.029.4417326
TACCTGG36400.028.425712
ACGCAGA424700.026.043777
CGCAGAG424500.026.0338928
TAACGCA7900.025.5994954
GCAGAGT443600.024.5950539
TCTGTCG10900.024.5817368
CATGGGT87150.024.068974
GTACACG4450.023.2620771
GTACATG184400.022.4292011
Produced by FastQC (version 0.11.5)