##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431445_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4329276
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25587142053313	33.0	31.0	34.0	30.0	34.0
2	32.37391379066615	34.0	31.0	34.0	31.0	34.0
3	32.48046001225147	34.0	31.0	34.0	31.0	34.0
4	36.034592851091034	37.0	35.0	37.0	35.0	37.0
5	35.952564816842354	37.0	35.0	37.0	35.0	37.0
6	35.996928354764165	37.0	35.0	37.0	35.0	37.0
7	35.93546680784501	37.0	35.0	37.0	35.0	37.0
8	35.922061333118975	37.0	35.0	37.0	35.0	37.0
9	37.60929009839059	39.0	37.0	39.0	35.0	39.0
10-11	37.54860939796862	39.0	37.0	39.0	35.0	39.0
12-13	37.523866692721825	39.0	37.0	39.0	35.0	39.0
14-15	38.839147007490396	40.0	38.0	41.0	35.0	41.0
16-17	38.89565541674867	40.0	38.0	41.0	35.0	41.0
18-19	38.992388103692164	40.5	38.5	41.0	35.0	41.0
20-21	38.998271419978764	41.0	39.0	41.0	35.0	41.0
22-23	38.902368432966625	40.0	38.0	41.0	35.0	41.0
24-25	38.83012286580943	40.0	38.0	41.0	35.0	41.0
26-27	38.66548840960937	40.0	38.0	41.0	34.5	41.0
28-29	38.54811058477215	40.0	38.0	41.0	34.0	41.0
30-31	38.31338658011178	40.0	38.0	41.0	34.0	41.0
32-33	38.03145283414594	40.0	37.0	41.0	33.5	41.0
34-35	37.82616689257049	40.0	37.0	41.0	33.0	41.0
36-37	37.63494150061119	40.0	37.0	41.0	33.0	41.0
38-39	37.50301586223655	40.0	36.5	41.0	33.0	41.0
40-41	37.34146078928671	40.0	36.0	41.0	33.0	41.0
42-43	37.17205994720595	40.0	36.0	41.0	32.0	41.0
44-45	36.89476866339776	40.0	36.0	41.0	31.0	41.0
46-47	36.481772702872256	40.0	36.0	41.0	30.0	41.0
48-49	36.148784115404055	40.0	35.0	41.0	29.0	41.0
50-51	35.98142183589127	40.0	35.0	41.0	28.5	41.0
52-53	35.832517954503246	40.0	35.0	41.0	28.0	41.0
54-55	35.6695143714561	39.5	35.0	41.0	28.0	41.0
56-57	35.44348604708963	39.0	35.0	41.0	27.0	41.0
58-59	35.27097937391841	39.0	35.0	41.0	27.0	41.0
60-61	35.05209647063389	39.0	35.0	41.0	26.0	41.0
62-63	34.80437086940172	38.0	35.0	41.0	26.0	41.0
64-65	34.55015850225303	37.5	35.0	40.0	26.0	41.0
66-67	34.28556333668725	37.0	34.0	40.0	26.0	41.0
68-69	33.98889433244727	36.5	34.0	40.0	25.5	41.0
70-71	33.6572506580777	36.0	34.0	39.0	24.5	41.0
72-73	33.33945964637043	35.5	34.0	39.0	24.0	41.0
74-75	33.040674237447554	35.0	34.0	38.5	24.0	40.5
76-77	32.701696773317295	35.0	34.0	37.5	23.0	40.0
78-79	32.39976095310163	35.0	33.0	37.0	22.5	39.0
80-81	32.11347035855418	35.0	33.0	36.5	22.0	39.0
82-83	31.872250464049877	35.0	33.0	36.0	22.0	38.5
84-85	31.6551130027284	35.0	33.0	36.0	20.5	37.0
86-87	31.42174176467382	35.0	33.0	35.5	20.0	37.0
88-89	31.28165286759264	35.0	33.0	35.0	20.0	36.5
90-91	31.10220831381506	35.0	33.0	35.0	19.5	36.0
92-93	30.9131310870455	35.0	33.0	35.0	17.5	36.0
94-95	30.751726039180685	35.0	33.0	35.0	10.0	36.0
96-97	30.585993824371556	35.0	33.0	35.0	2.0	36.0
98-99	30.42565847037703	35.0	33.0	35.0	2.0	35.0
100	30.318440773930792	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.16415129562122388
1101	2	0.2348444915877934
1101	3	0.20657617733351685
1101	4	0.18377734763218712
1101	5	0.14694244311491644
1101	6	0.16270122075933102
1101	7	0.14924812894564354
1101	8	0.23464890313942988
1101	9	0.34701058907463533
1101	10-11	0.2512090528688944
1101	12-13	0.30320127344835157
1101	14-15	0.42447086242902543
1101	16-17	0.4092080423375748
1101	18-19	0.38065220837151514
1101	20-21	0.35267548527404813
1101	22-23	0.37143833404807935
1101	24-25	0.37249568388597254
1101	26-27	0.3741716326132547
1101	28-29	0.3906067725641833
1101	30-31	0.3842910042870713
1101	32-33	0.4450214705140425
1101	34-35	0.5491016582740258
1101	36-37	0.6137505478920815
1101	38-39	0.5440567515396069
1101	40-41	0.6258101070989426
1101	42-43	0.6350264716223819
1101	44-45	0.6158401351987379
1101	46-47	0.6512039969420726
1101	48-49	0.5944029771995787
1101	50-51	0.6017191687195549
1101	52-53	0.604129061914243
1101	54-55	0.5353861031395937
1101	56-57	0.48742525726272845
1101	58-59	0.5481734066649295
1101	60-61	0.6164222839089035
1101	62-63	0.661618617668914
1101	64-65	0.6208626040755263
1101	66-67	0.5984907498085832
1101	68-69	0.5004643091925658
1101	70-71	0.5630560297570852
1101	72-73	0.629755665567501
1101	74-75	0.5565532868248582
1101	76-77	0.602008729529345
1101	78-79	0.5643282107087444
1101	80-81	0.5504469493939084
1101	82-83	0.5512899297564893
1101	84-85	0.5277016653309659
1101	86-87	0.4774660232956407
1101	88-89	0.468974702534652
1101	90-91	0.5103772897532544
1101	92-93	0.5022826560366127
1101	94-95	0.5870228231017123
1101	96-97	0.5448844525386001
1101	98-99	0.5752770287446722
1101	100	0.6001640518576359
1102	1	0.12355394712879075
1102	2	0.17443928453000268
1102	3	0.20555941572888514
1102	4	0.14391907786803415
1102	5	0.10128415624751597
1102	6	0.1260162336476469
1102	7	0.18256551137584864
1102	8	0.2019763216222188
1102	9	0.30264277179220755
1102	10-11	0.27903265314215986
1102	12-13	0.28766147347239723
1102	14-15	0.39902622342307126
1102	16-17	0.38646685047620366
1102	18-19	0.38562828831507545
1102	20-21	0.3650245074566243
1102	22-23	0.33521759799531026
1102	24-25	0.31142310018536534
1102	26-27	0.3839660982983588
1102	28-29	0.3837309022684039
1102	30-31	0.3997260293632081
1102	32-33	0.40889467636984733
1102	34-35	0.47655518733087376
1102	36-37	0.40755960536445457
1102	38-39	0.42547083759134097
1102	40-41	0.4549446392739753
1102	42-43	0.46259609843423277
1102	44-45	0.3931419953800912
1102	46-47	0.44023906434244964
1102	48-49	0.45543243887689044
1102	50-51	0.40211661633245654
1102	52-53	0.4036270773354431
1102	54-55	0.4302788376315192
1102	56-57	0.4679870360343017
1102	58-59	0.4300377756550873
1102	60-61	0.3827010016578356
1102	62-63	0.38065509447783796
1102	64-65	0.422009249606198
1102	66-67	0.38329973296160347
1102	68-69	0.36880045551129115
1102	70-71	0.392896833772177
1102	72-73	0.41564191552043894
1102	74-75	0.411896841705925
1102	76-77	0.4208318775881992
1102	78-79	0.393955839931003
1102	80-81	0.41927386940513856
1102	82-83	0.42009997045579084
1102	84-85	0.3997881120412572
1102	86-87	0.3518725916153542
1102	88-89	0.2711011039479203
1102	90-91	0.2649825776805841
1102	92-93	0.31421824704556656
1102	94-95	0.30076044161554805
1102	96-97	0.31879109370808223
1102	98-99	0.42363706922702704
1102	100	0.4428465692152628
1103	1	0.09106506750688226
1103	2	0.15513752807497383
1103	3	0.16052101567328236
1103	4	0.1237422160734809
1103	5	0.08263189394559589
1103	6	0.12828461702268612
1103	7	0.1390325433643156
1103	8	0.1382660574263639
1103	9	0.20104959301475134
1103	10-11	0.21213958795762977
1103	12-13	0.2107542444664574
1103	14-15	0.2802492505333376
1103	16-17	0.3190808750756986
1103	18-19	0.2597634725832165
1103	20-21	0.29674463935907625
1103	22-23	0.2617987903300687
1103	24-25	0.3486984120772263
1103	26-27	0.29829949461820604
1103	28-29	0.2877654465718109
1103	30-31	0.253126907590719
1103	32-33	0.24281731372389714
1103	34-35	0.2792427296838724
1103	36-37	0.3380492332227476
1103	38-39	0.3260474357113097
1103	40-41	0.29831978805246706
1103	42-43	0.33453359626228263
1103	44-45	0.2946282056768652
1103	46-47	0.26889590674149844
1103	48-49	0.15065139575501973
1103	50-51	0.15860076599276596
1103	52-53	0.14860000670801554
1103	54-55	0.09696677582219593
1103	56-57	0.1200232516523414
1103	58-59	0.1471734475257449
1103	60-61	0.16758588022418053
1103	62-63	0.16711576265618078
1103	64-65	0.15781245529409915
1103	66-67	0.20667679848495624
1103	68-69	0.11885628817131533
1103	70-71	0.16182759421491255
1103	72-73	0.14921590078593994
1103	74-75	0.1512647177137154
1103	76-77	0.20945901044196802
1103	78-79	0.17911392251284752
1103	80-81	0.16140079918169903
1103	82-83	0.14714449377816763
1103	84-85	0.15015299944742821
1103	86-87	0.19537493514815907
1103	88-89	0.14995677468916568
1103	90-91	0.20832949112407917
1103	92-93	0.16068003377756312
1103	94-95	0.132855927002673
1103	96-97	0.15208935006754842
1103	98-99	0.22413459483430387
1103	100	0.18623317502392567
1104	1	0.07752439892389873
1104	2	0.16038677244245036
1104	3	0.13755344673896985
1104	4	0.11272732300151489
1104	5	0.11479264530299105
1104	6	0.11656877810008126
1104	7	0.11052924885643733
1104	8	0.11225610070992076
1104	9	0.195069096401582
1104	10-11	0.20006792180664235
1104	12-13	0.23000486140693255
1104	14-15	0.2626202596541489
1104	16-17	0.21328184774983328
1104	18-19	0.1671353810190226
1104	20-21	0.21132382960547602
1104	22-23	0.20433950278714974
1104	24-25	0.1785927233329403
1104	26-27	0.18493496701121614
1104	28-29	0.23554043214119247
1104	30-31	0.16785097592119058
1104	32-33	0.12340439902012434
1104	34-35	0.23368691559691968
1104	36-37	0.22387131940061522
1104	38-39	0.2231704802990322
1104	40-41	0.24774683603700964
1104	42-43	0.2720203924469473
1104	44-45	0.3076237750170705
1104	46-47	0.3119259193256738
1104	48-49	0.18872898989926057
1104	50-51	0.19414374742674312
1104	52-53	0.16477330293758286
1104	54-55	0.17286316413164116
1104	56-57	0.19714598329925082
1104	58-59	0.25542835851331347
1104	60-61	0.23661727224317275
1104	62-63	0.12176835052081714
1104	64-65	0.13642323613654384
1104	66-67	0.13523240921235669
1104	68-69	0.1677390738478124
1104	70-71	0.2089819536780766
1104	72-73	0.19275064740576653
1104	74-75	0.25644610278770585
1104	76-77	0.23976187061709453
1104	78-79	0.22680134703276877
1104	80-81	0.1550109323094233
1104	82-83	0.21923594797777568
1104	84-85	0.21780311123280072
1104	86-87	0.16127717368350858
1104	88-89	0.1633418565819298
1104	90-91	0.22710422488525595
1104	92-93	0.22149431202998926
1104	94-95	0.2168210680984366
1104	96-97	0.2650231421866778
1104	98-99	0.2297506089621777
1104	100	0.26979903066442645
1105	1	0.10313993760608042
1105	2	0.1515564144954169
1105	3	0.11190416399638536
1105	4	0.07913709729297835
1105	5	0.0544338247438958
1105	6	0.09869036021273558
1105	7	0.10586555317576085
1105	8	0.11058325525937818
1105	9	0.20357672333872046
1105	10-11	0.16391122394507107
1105	12-13	0.0925382477853276
1105	14-15	0.21791581663758564
1105	16-17	0.18783997028462096
1105	18-19	0.19070831025641866
1105	20-21	0.14582571572746872
1105	22-23	0.13408210601116366
1105	24-25	0.14826583994290843
1105	26-27	0.1765797666718214
1105	28-29	0.17707709994087395
1105	30-31	0.1627813834483689
1105	32-33	0.10603106082081126
1105	34-35	0.17805287953724758
1105	36-37	0.16064183982504687
1105	38-39	0.1506732020768382
1105	40-41	0.12946358245984868
1105	42-43	0.16405832046910263
1105	44-45	0.08691408462309624
1105	46-47	0.02185415611906194
1105	48-49	-0.03823721891934184
1105	50-51	-0.03746268458342428
1105	52-53	-0.01613932906342086
1105	54-55	0.018624620981917417
1105	56-57	0.049757534700781036
1105	58-59	-0.029790948460124866
1105	60-61	-0.02592783655024533
1105	62-63	-0.010128255264220343
1105	64-65	-0.0014336971941943943
1105	66-67	-0.03152157209644457
1105	68-69	-0.012540303839294609
1105	70-71	-0.02413460046899729
1105	72-73	-0.002637645899618235
1105	74-75	-0.019727055235435387
1105	76-77	0.034071706769310595
1105	78-79	0.02388888259540778
1105	80-81	6.124943067220556E-5
1105	82-83	-0.04926139179841016
1105	84-85	-0.035437852386927204
1105	86-87	-0.0403508917393367
1105	88-89	-0.032559927281980094
1105	90-91	-0.015932605273139444
1105	92-93	0.028538872444705987
1105	94-95	0.044553365152179225
1105	96-97	0.03044911870634337
1105	98-99	0.08159287373477042
1105	100	0.080462968437363
1106	1	0.03044779317427526
1106	2	0.11718361468359717
1106	3	0.10125248705585221
1106	4	0.13261395214131255
1106	5	0.06460398416061963
1106	6	0.050273229778476036
1106	7	0.09134937119878828
1106	8	0.09568511239545785
1106	9	0.07651205779480108
1106	10-11	0.13049102020872283
1106	12-13	0.1648319939357208
1106	14-15	0.2212895305157403
1106	16-17	0.18733184626187693
1106	18-19	0.18280675660861334
1106	20-21	0.15520944395325387
1106	22-23	0.1713250220952247
1106	24-25	0.1763622660166675
1106	26-27	0.24076203892717984
1106	28-29	0.21963205444387768
1106	30-31	0.1860665473652503
1106	32-33	0.24961064644047326
1106	34-35	0.30737055985876793
1106	36-37	0.24734552595737114
1106	38-39	0.2904905993071836
1106	40-41	0.2737431680157272
1106	42-43	0.31219519823460473
1106	44-45	0.2722423337476556
1106	46-47	0.3148463575812599
1106	48-49	0.38204915827352437
1106	50-51	0.3754421984954277
1106	52-53	0.3798193363359488
1106	54-55	0.37706104499191895
1106	56-57	0.39120607100969096
1106	58-59	0.3830955590837348
1106	60-61	0.3726503361895013
1106	62-63	0.3856489854605343
1106	64-65	0.3659382171082015
1106	66-67	0.4142597804302639
1106	68-69	0.39626265198695165
1106	70-71	0.419268113879653
1106	72-73	0.3897779268584429
1106	74-75	0.38401180922716094
1106	76-77	0.4100129132024364
1106	78-79	0.3475550530490352
1106	80-81	0.3165924253535266
1106	82-83	0.37219698388574685
1106	84-85	0.37060719078429116
1106	86-87	0.3820288244373984
1106	88-89	0.32267276268191836
1106	90-91	0.36036146540539704
1106	92-93	0.41532983868823337
1106	94-95	0.3969600376744822
1106	96-97	0.384576958161972
1106	98-99	0.4278728754756642
1106	100	0.4912113512308167
1107	1	0.07053852969433905
1107	2	0.12420907914204804
1107	3	0.09515199818594056
1107	4	0.09715796475042993
1107	5	0.08268582284573256
1107	6	0.08443922279043647
1107	7	-0.004724377511202249
1107	8	0.04607025836784118
1107	9	0.13406195951076683
1107	10-11	0.093411294339937
1107	12-13	0.09616450707623159
1107	14-15	0.17662805222118294
1107	16-17	0.11295124887487873
1107	18-19	0.07913056900329707
1107	20-21	0.11579583975647978
1107	22-23	0.1473572076127212
1107	24-25	0.16185125137388923
1107	26-27	0.15715632776937838
1107	28-29	0.1540176921112959
1107	30-31	0.22289298050019823
1107	32-33	0.24576258068393741
1107	34-35	0.24384432394199962
1107	36-37	0.23853011422922066
1107	38-39	0.16068749625548406
1107	40-41	0.11672747435883934
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2216	68-69	-0.948037662559365
2216	70-71	-1.020718210909486
2216	72-73	-0.9306484210986881
2216	74-75	-0.9932833888472743
2216	76-77	-0.9344769277460578
2216	78-79	-0.8878495615319011
2216	80-81	-0.8798201576517499
2216	82-83	-0.9339677081359063
2216	84-85	-0.9503919146873159
2216	86-87	-1.008909125425376
2216	88-89	-1.0061292608540953
2216	90-91	-1.0024791335869487
2216	92-93	-1.0066789559296154
2216	94-95	-1.0758435205149404
2216	96-97	-1.2327381969932176
2216	98-99	-1.3162883199402984
2216	100	-1.3407929234046314
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	302.0
3	851.0
4	530.0
5	498.0
6	895.0
7	1965.0
8	3379.0
9	4929.0
10	6936.0
11	9671.0
12	13897.0
13	19339.0
14	20117.0
15	24454.0
16	31426.0
17	44547.0
18	50176.0
19	28037.0
20	18032.0
21	16143.0
22	16118.0
23	16305.0
24	18529.0
25	21812.0
26	26711.0
27	34039.0
28	42699.0
29	53279.0
30	67408.0
31	83946.0
32	105618.0
33	134763.0
34	182992.0
35	286275.0
36	429798.0
37	798498.0
38	1159311.0
39	551850.0
40	3201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.60450533413907	20.360731770162328	14.252301947050308	23.782460948648296
2	14.77337088233691	21.690439694766514	39.1654632321894	24.370726190707177
3	16.41998552643827	27.320868884914397	31.528526845038417	24.730618743608915
4	11.274532918745846	18.423948067462	37.680006694053105	32.62151231973904
5	11.47836696716203	37.49979787825295	34.828187275153994	16.193647879431026
6	28.02325377268624	38.60005691482825	18.34244340162189	15.034245910863616
7	24.538375469709024	33.96299981798342	23.125968406726667	18.372656305580886
8	25.187352342516395	33.437808076916326	21.588043820721985	19.786795759845297
9	25.155014371918078	15.512986467021275	22.93868535986156	36.39331380119909
10-11	22.95282927880087	26.596855131246034	29.190878067176858	21.259437522776246
12-13	24.90988100550762	24.922238729986258	29.514738723056695	20.653141541449425
14-15	22.177368687050674	26.121711805853913	28.86670889081685	22.834210616278565
16-17	19.492162199868986	29.152588100181188	29.31633372416081	22.038915975789024
18-19	19.231541255396976	26.233982310206137	34.04385629375442	20.490620140642456
20-21	20.972733085162506	25.523135970079064	33.830864560263656	19.673266384494774
22-23	20.422895190789404	25.830866408147692	33.92488720977826	19.821351191284638
24-25	20.104325169298857	25.6112804693189	34.189655142544815	20.094739218837432
26-27	19.91660961324711	25.421964319207184	34.2872226210572	20.37420344648851
28-29	19.918937591369605	25.588651817159384	34.24170706428378	20.250703527187227
30-31	20.397140391451018	26.16700283937351	33.681490758869465	19.75436601030601
32-33	19.702405034008592	25.763941686751828	34.320261817600574	20.213391461639002
34-35	19.953910749053698	25.323944859211	34.00564629704872	20.71649809468658
36-37	20.685932185693336	25.953046765205645	33.161054127447734	20.19996692165329
38-39	20.50177601174345	25.883192651798332	33.34172591036843	20.273305426089788
40-41	20.168868308382216	25.767448564485406	33.47090586400843	20.592777263123953
42-43	20.15686742830137	25.95974495963108	33.18163853481373	20.70174907725382
44-45	20.65901095922401	26.123204679397283	32.647541141699115	20.5702432196796
46-47	20.59187944360006	26.737906709229755	31.87977853028	20.790435316890186
48-49	20.64325632083495	26.349089242627024	31.664774115817647	21.342880320720383
50-51	20.690571561423084	26.32552444313756	31.386746915315516	21.59715708012384
52-53	20.76942000145522	26.663198001494493	31.062774370067693	21.504607626982597
54-55	20.956423900688137	27.205720831251558	30.73410728140864	21.103747986651673
56-57	20.520221099280192	26.738762517164737	30.892164493669505	21.848851889885566
58-59	20.420830889961707	26.161011077092883	31.430146560716455	21.98801147222896
60-61	20.95549544721682	26.717757471322777	30.776086685361335	21.550660396099065
62-63	20.690681492640948	26.47891888470717	31.096905888649506	21.733493734002376
64-65	20.54819343900287	26.254163567571066	31.466938617118096	21.730704376307973
66-67	20.557218331717397	26.3785081673653	30.944217533084366	22.120055967832933
68-69	21.159260808157736	26.506698690752177	30.70907455728677	21.62496594380332
70-71	21.166255010383995	26.240968720051168	30.795373988943602	21.797402280621235
72-73	20.831000379740168	26.35951138250368	31.06132526547164	21.74816297228451
74-75	20.840054545036672	26.337163581867735	30.756526538618033	22.06625533447756
76-77	20.99401839938133	26.428830132336216	30.405106997105293	22.172044471177166
78-79	21.09413203075175	26.54851915424592	30.507254260496453	21.850094554505876
80-81	20.71556537397939	26.384642605368658	30.667795261840546	22.231996758811405
82-83	20.52929468106706	26.409585716453698	30.776248356233076	22.28487124624616
84-85	20.65371876448722	26.285032939908486	30.51005174802916	22.551196547575135
86-87	20.84855527806497	26.401238913850722	30.31919193879069	22.43101386929362
88-89	20.41921097199624	26.28582700664037	30.48814628589168	22.806815735471705
90-91	20.268342789879878	26.128283343450498	30.384849568380485	23.218524298289136
92-93	20.3905972546164	26.379092998988856	30.192059049250247	23.038250697144495
94-95	20.25606591032773	26.085862855590637	30.502836963963492	23.155234270118143
96-97	20.167275758654743	26.173003991330155	30.389488176785935	23.27023207322917
98-99	20.074883545219265	26.059838695128988	30.396311790791742	23.468965968860005
100	20.251779517257436	26.18696850088144	30.11544225531176	23.445809726549363
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4603.0
1	2882.5
2	8830.5
3	15088.0
4	8650.5
5	3783.5
6	6686.0
7	15412.0
8	23800.5
9	24671.0
10	16398.0
11	7845.5
12	5978.0
13	6181.0
14	6725.5
15	7199.0
16	7704.5
17	8423.5
18	9201.5
19	9972.0
20	11112.5
21	12639.5
22	14870.0
23	18512.0
24	22917.0
25	28524.5
26	36702.5
27	46666.0
28	59031.5
29	71661.0
30	81185.0
31	89898.0
32	98131.0
33	105636.5
34	112453.5
35	118342.5
36	123284.5
37	127008.5
38	129266.0
39	130228.0
40	131241.0
41	130919.0
42	127941.0
43	125804.5
44	125552.5
45	125092.0
46	124981.0
47	124610.5
48	124568.0
49	126006.5
50	127895.0
51	124414.5
52	117744.0
53	113995.5
54	111740.5
55	109741.5
56	103184.0
57	93397.0
58	87600.0
59	82591.0
60	72588.0
61	61270.0
62	51301.0
63	40770.0
64	30888.0
65	23577.0
66	18203.5
67	13823.0
68	10454.5
69	7885.0
70	5763.5
71	4236.0
72	3393.5
73	2763.0
74	2251.5
75	1753.0
76	1165.0
77	848.0
78	648.0
79	432.0
80	233.5
81	92.0
82	45.0
83	24.5
84	12.5
85	8.5
86	6.0
87	4.5
88	3.0
89	1.5
90	1.5
91	1.0
92	0.5
93	0.5
94	1.0
95	1.5
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04529625738807135
2	0.0
3	6.929565128210814E-5
4	0.07040438170262187
5	0.004665907186328615
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.01105265637949625
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.428159350431803E-4
26-27	0.0
28-29	4.388724581200182E-4
30-31	0.0035109796649601455
32-33	5.774637606842345E-5
34-35	0.0015476028786337486
36-37	0.003695768068379101
38-39	0.001235772447864262
40-41	0.0010278854940179375
42-43	0.0018247854837621811
44-45	1.154927521368469E-5
46-47	3.6957680683791006E-4
48-49	5.081681094021263E-4
50-51	0.0015014057777790097
52-53	9.470405675221445E-4
54-55	0.001039434769231622
56-57	0.006248157890603416
58-59	0.008950688290605635
60-61	0.0012935188239326853
62-63	0.0027025304000022174
64-65	0.0012935188239326853
66-67	8.084492649579283E-5
68-69	7.968999897442436E-4
70-71	3.9267535726527947E-4
72-73	0.0
74-75	2.194362290600091E-4
76-77	0.0
78-79	6.236608615389732E-4
80-81	0.0
82-83	9.932376683768832E-4
84-85	6.69857962393712E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	1.9633767863263974E-4
94-95	0.0
96-97	4.042246324789641E-4
98-99	4.7352028376107226E-4
100	2.7718260512843255E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4329276.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.72893524280802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.00073469132826	41.41253950981769
2	16.565499586548206	18.794863066201124
3	5.173105271223742	8.803942618064413
4	2.090335199418335	4.743299606542596
5	1.010507558325456	2.8662508919306418
6	0.5650434233608113	1.923258706392601
7	0.35308538936596534	1.402111073196645
8	0.23061722829263837	1.0466135847751163
9	0.15649258358495252	0.7989891876153444
>10	0.7703808507190644	7.599376514642742
>50	0.04845004765781792	1.8774778583808014
>100	0.03240245351760009	3.487133321326897
>500	0.002284879855854208	0.8653674758506616
>1k	7.344254778004487E-4	0.6544821926583037
>5k	1.2240424630005146E-4	0.5375706933260431
>10k+	2.0400707716675243E-4	3.1867236992784918
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40375	0.9326039735050387	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35178	0.812560806934	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25982	0.6001465372039112	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23731	0.5481517001919027	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12412	0.28669920790450876	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8742	0.2019275278360631	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8066	0.18631290774716142	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6417	0.14822339809242932	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.929565128210814E-5	0.0
10-11	0.0	0.0	0.0	1.3859130256421628E-4	0.0
12-13	0.0	0.0	0.0	2.0788695384632442E-4	0.0
14-15	0.0	0.0	0.0	2.7718260512843255E-4	0.0
16-17	0.0	0.0	0.0	3.002811555558019E-4	0.0
18-19	0.0	0.0	0.0	3.118304307694866E-4	0.0
20-21	0.0	0.0	0.0	4.504217333337029E-4	0.0
22-23	0.0	0.0	0.0	0.0011780260717958384	0.0
24-25	0.0	0.0	0.0	0.0024022492444464153	0.0
26-27	0.0	0.0	0.0	0.003765063719661209	0.0
28-29	0.0	0.0	0.0	0.008650407135049833	0.0
30-31	0.0	0.0	0.0	0.020257428724802948	0.0
32-33	0.0	0.0	0.0	0.04004133716584482	0.0
34-35	0.0	0.0	0.0	0.07087790198638294	0.0
36-37	0.0	0.0	0.0	0.11552739996248795	0.0
38-39	0.0	0.0	0.0	0.17904841363775376	0.0
40-41	0.0	0.0	0.0	0.2544305329574737	0.0
42-43	0.0	0.0	0.0	0.3302515247353137	0.0
44-45	0.0	0.0	0.0	0.4231769931046207	0.0
46-47	0.0	0.0	0.0	0.5357015815115507	0.0
48-49	0.0	0.0	0.0	0.6571537596586589	0.0
50-51	0.0	0.0	0.0	0.7843921246878232	0.0
52-53	0.0	0.0	0.0	0.9094130288759599	0.0
54-55	0.0	2.309855042736938E-5	0.0	1.0490437662094076	0.0
56-57	0.0	2.309855042736938E-5	0.0	1.219534166913821	0.0
58-59	0.0	2.309855042736938E-5	0.0	1.4080414369515828	0.0
60-61	0.0	2.309855042736938E-5	0.0	1.6091027691466193	0.0
62-63	0.0	2.309855042736938E-5	0.0	1.813328602750206	0.0
64-65	0.0	2.309855042736938E-5	0.0	2.0421197447332995	0.0
66-67	0.0	2.309855042736938E-5	0.0	2.3092544804258264	0.0
68-69	0.0	2.309855042736938E-5	0.0	2.6061285997935912	0.0
70-71	0.0	2.309855042736938E-5	0.0	2.922440149345988	0.0
72-73	0.0	2.309855042736938E-5	0.0	3.2681792521428523	0.0
74-75	0.0	2.309855042736938E-5	0.0	3.621379186727758	0.0
76-77	0.0	2.309855042736938E-5	0.0	3.994108945699004	0.0
78-79	0.0	2.309855042736938E-5	0.0	4.4186949503796935	0.0
80-81	0.0	2.309855042736938E-5	0.0	4.873551605395452	0.0
82-83	0.0	2.309855042736938E-5	0.0	5.351772444168494	2.309855042736938E-5
84-85	0.0	2.309855042736938E-5	0.0	5.8340470785415395	2.309855042736938E-5
86-87	0.0	2.309855042736938E-5	0.0	6.344167015454778	2.309855042736938E-5
88	0.0	2.309855042736938E-5	0.0	6.756164310152552	2.309855042736938E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13675	0.0	63.379402	1
GTATCAA	25930	0.0	43.13328	1
ATAACGC	425	0.0	40.945183	3
TATAACG	480	0.0	39.193027	2
GTGGTAT	4610	0.0	36.131683	1
TGGTATC	4795	0.0	34.0354	2
TATCAAC	34650	0.0	32.033085	2
ATCAACG	34785	0.0	31.841166	3
TCAACGC	35115	0.0	31.528536	4
CAACGCA	36465	0.0	30.424612	5
AACGCAG	37760	0.0	29.441732	6
TACCTGG	3640	0.0	28.42571	2
ACGCAGA	42470	0.0	26.04377	7
CGCAGAG	42450	0.0	26.033892	8
TAACGCA	790	0.0	25.599495	4
GCAGAGT	44360	0.0	24.595053	9
TCTGTCG	1090	0.0	24.581736	8
CATGGGT	8715	0.0	24.06897	4
GTACACG	445	0.0	23.262077	1
GTACATG	18440	0.0	22.429201	1
>>END_MODULE