FastQCFastQC Report
Wed 25 May 2016
SRR2431444_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431444_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7141576
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA211660.296377158207096No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA84580.11843324218631854No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA79840.11179605173984004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81850.043.36571
GTATCAA139150.034.326431
ATCAACG150000.030.1793983
TCAACGC150950.029.9890464
TATCAAC166900.027.5741352
GTGGTAT42150.025.2074551
GTACATG297200.024.3607161
TACATGG296200.023.1709232
TGGTATC45050.022.956382
ACGCAGA195300.022.5504447
CGCAGAG194650.022.2630738
CAACGCA204600.022.1481695
ACATGGG307800.021.7936953
AACGCAG213500.021.2229636
CATGGGT69750.020.2183864
ATGCGGG21300.019.4183228
TACACGC34600.019.0203445
GCAGAGT219450.018.804759
TTACACG36350.018.4927334
CATGGGG183550.017.7735654
Produced by FastQC (version 0.11.5)