##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431444_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7141576
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.300680129988116	31.0	31.0	34.0	26.0	34.0
2	30.388978567195814	31.0	31.0	34.0	26.0	34.0
3	30.463129426894007	31.0	31.0	34.0	26.0	34.0
4	33.84804824593339	37.0	35.0	37.0	28.0	37.0
5	33.88615300040215	37.0	35.0	37.0	30.0	37.0
6	33.98014275840515	37.0	35.0	37.0	30.0	37.0
7	33.850270444506926	37.0	35.0	37.0	29.0	37.0
8	33.822922419365135	37.0	35.0	37.0	29.0	37.0
9	35.19194754771216	39.0	35.0	39.0	28.0	39.0
10-11	35.13934137226853	39.0	35.0	39.0	27.5	39.0
12-13	35.09412628249002	39.0	35.0	39.0	27.0	39.0
14-15	36.28823301747401	40.0	37.0	41.0	27.0	41.0
16-17	36.29891497338963	40.0	37.0	41.0	27.0	41.0
18-19	36.287378584222864	40.0	36.5	41.0	27.0	41.0
20-21	36.37093969174311	40.0	37.0	41.0	27.0	41.0
22-23	36.32196471759175	40.0	37.0	41.0	27.0	41.0
24-25	36.333518399860196	40.0	36.5	41.0	28.0	41.0
26-27	36.30555468428817	40.0	36.0	41.0	28.0	41.0
28-29	36.19740810711809	40.0	36.0	41.0	27.5	41.0
30-31	36.176939725909236	40.0	36.0	41.0	28.0	41.0
32-33	36.1294173722999	40.0	36.0	41.0	28.0	41.0
34-35	36.02688720248864	40.0	36.0	41.0	27.5	41.0
36-37	35.90528400173855	40.0	36.0	41.0	27.0	41.0
38-39	35.80649677326126	40.0	35.5	41.0	27.0	41.0
40-41	35.712507645371275	40.0	35.0	41.0	27.0	41.0
42-43	35.48852627207216	40.0	35.0	41.0	26.0	41.0
44-45	35.3431250329059	39.5	35.0	41.0	26.0	41.0
46-47	35.205749473225524	39.0	35.0	41.0	25.0	41.0
48-49	35.06501184052372	39.0	35.0	41.0	25.0	41.0
50-51	34.88110355473358	39.0	34.0	41.0	24.0	41.0
52-53	34.76319323633887	39.0	34.0	41.0	24.0	41.0
54-55	34.68029780821488	39.0	34.0	41.0	24.0	41.0
56-57	34.50583946736687	38.0	34.0	40.5	24.0	41.0
58-59	34.2933178194841	38.0	34.0	40.0	23.5	41.0
60-61	34.08427145492816	38.0	33.5	40.0	23.0	41.0
62-63	33.825357666151	37.0	33.0	40.0	22.5	41.0
64-65	33.69116032651616	37.0	33.0	40.0	22.0	41.0
66-67	33.44390215829111	36.5	33.0	40.0	22.0	41.0
68-69	33.19458240029932	36.0	33.0	39.5	22.0	41.0
70-71	32.9204410203014	36.0	33.0	39.0	20.5	41.0
72-73	32.61569211053694	35.0	32.5	39.0	20.0	41.0
74-75	32.277129095874635	35.0	32.0	38.5	18.5	40.0
76-77	31.96370703049299	35.0	32.0	37.5	18.0	40.0
78-79	31.63481057962556	35.0	32.0	37.0	16.0	39.0
80-81	31.340834992164194	35.0	32.0	36.5	13.5	39.0
82-83	31.04300003248583	35.0	31.5	36.0	9.5	38.5
84-85	30.75768604856967	35.0	31.0	36.0	7.0	37.0
86-87	30.507462918549074	35.0	31.0	35.0	3.5	37.0
88-89	30.249434788623688	35.0	31.0	35.0	2.0	36.5
90-91	30.03433856896573	35.0	31.0	35.0	2.0	36.0
92-93	29.858906143405882	35.0	31.0	35.0	2.0	36.0
94-95	29.633579618840436	34.5	31.0	35.0	2.0	36.0
96-97	29.550629580921637	35.0	31.0	35.0	2.0	35.5
98-99	29.407651966456704	35.0	31.0	35.0	2.0	35.0
100	29.280618731775732	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3046202373269935
1101	2	0.4657443027058932
1101	3	0.3911627693893962
1101	4	0.440709854629425
1101	5	0.3357441751892196
1101	6	0.41378492862175165
1101	7	0.4015070424067133
1101	8	0.522052239357059
1101	9	0.6358047621961589
1101	10-11	0.7042840547344582
1101	12-13	0.6330284079753241
1101	14-15	0.7302069826801088
1101	16-17	0.6767257566666558
1101	18-19	0.651299949410955
1101	20-21	0.6395636160775666
1101	22-23	0.5840987300813936
1101	24-25	0.6483492062992013
1101	26-27	0.7417327720520106
1101	28-29	0.7792269810197539
1101	30-31	0.7394764784424339
1101	32-33	0.7351635063747111
1101	34-35	0.6996842738954072
1101	36-37	0.6965963973449831
1101	38-39	0.6763604449103013
1101	40-41	0.6440045809772599
1101	42-43	0.712294530565103
1101	44-45	0.5784499625433099
1101	46-47	0.5299786989417115
1101	48-49	0.5734470561735208
1101	50-51	0.5556679939165861
1101	52-53	0.4867826437050198
1101	54-55	0.5346220534066859
1101	56-57	0.5628958932983821
1101	58-59	0.5731178615061765
1101	60-61	0.5636161353062974
1101	62-63	0.537696062164585
1101	64-65	0.5203744647479454
1101	66-67	0.591895116054836
1101	68-69	0.5762753991379199
1101	70-71	0.5738597233498268
1101	72-73	0.5763440768266292
1101	74-75	0.5559223296868794
1101	76-77	0.5627567908757278
1101	78-79	0.521754347887466
1101	80-81	0.5477869266530035
1101	82-83	0.5408928393242967
1101	84-85	0.561800382967288
1101	86-87	0.5474243523975524
1101	88-89	0.5433660329346424
1101	90-91	0.5203626830711947
1101	92-93	0.5113029820558594
1101	94-95	0.5275661396311797
1101	96-97	0.6045349377452283
1101	98-99	0.641158416239243
1101	100	0.65465518236104
1102	1	0.2561330680017164
1102	2	0.3709405575359739
1102	3	0.3302485020754169
1102	4	0.3495619501411653
1102	5	0.2939823997012567
1102	6	0.27813532988996315
1102	7	0.3392236380670681
1102	8	0.3136309077410928
1102	9	0.46134732634953224
1102	10-11	0.4586884736386452
1102	12-13	0.4752776847017657
1102	14-15	0.613436295074834
1102	16-17	0.5755847232771814
1102	18-19	0.5710800841578063
1102	20-21	0.548126367489445
1102	22-23	0.5314055615487376
1102	24-25	0.5305051411055075
1102	26-27	0.5986494023898743
1102	28-29	0.6736813404767972
1102	30-31	0.6492648317566108
1102	32-33	0.6475865322342145
1102	34-35	0.608112967049081
1102	36-37	0.5962008112952404
1102	38-39	0.5425428584642447
1102	40-41	0.581353456435501
1102	42-43	0.6100627709297086
1102	44-45	0.501270154755737
1102	46-47	0.47992963028026026
1102	48-49	0.47105154443271147
1102	50-51	0.46996997548797026
1102	52-53	0.4716578740626929
1102	54-55	0.4878514995574861
1102	56-57	0.5316370208124965
1102	58-59	0.4966298549669901
1102	60-61	0.49786337596814434
1102	62-63	0.5021141440034853
1102	64-65	0.4661605788864591
1102	66-67	0.488577485023427
1102	68-69	0.4967696624887523
1102	70-71	0.4776671393807277
1102	72-73	0.4493456571298111
1102	74-75	0.45125032433664103
1102	76-77	0.3869885257414758
1102	78-79	0.3129927556948502
1102	80-81	0.3032601159921491
1102	82-83	0.2983588850985974
1102	84-85	0.27215603539966793
1102	86-87	0.2618356176308829
1102	88-89	0.3400625949082894
1102	90-91	0.3210216249120741
1102	92-93	0.32315114910232623
1102	94-95	0.3178713759335672
1102	96-97	0.3315190504966381
1102	98-99	0.36371876004188053
1102	100	0.33909905435152865
1103	1	0.2302581549649112
1103	2	0.29010887147046205
1103	3	0.3236419457685713
1103	4	0.3242590387786066
1103	5	0.2227454183904598
1103	6	0.30944335908018417
1103	7	0.3081328388325062
1103	8	0.3492911670959913
1103	9	0.42350864720004466
1103	10-11	0.5251303639807645
1103	12-13	0.416907623204537
1103	14-15	0.5509284667515928
1103	16-17	0.4531122757031767
1103	18-19	0.39369746300847197
1103	20-21	0.3966962112601635
1103	22-23	0.43781912635178344
1103	24-25	0.4587521977521902
1103	26-27	0.44574866171790717
1103	28-29	0.5005969499397196
1103	30-31	0.43266579468175337
1103	32-33	0.5277318047430128
1103	34-35	0.5155018341129747
1103	36-37	0.538151292449875
1103	38-39	0.5715250913749514
1103	40-41	0.4931322422049149
1103	42-43	0.49403590585647805
1103	44-45	0.47977886697221095
1103	46-47	0.4629373236503369
1103	48-49	0.3757985713250349
1103	50-51	0.3433110397596266
1103	52-53	0.3709639179862947
1103	54-55	0.4676529003375336
1103	56-57	0.47030094380343
1103	58-59	0.4696341396724577
1103	60-61	0.4847226621128229
1103	62-63	0.4161053861739106
1103	64-65	0.49852967065314857
1103	66-67	0.49999251310223514
1103	68-69	0.4570756322381513
1103	70-71	0.4418311685712695
1103	72-73	0.43750340083595063
1103	74-75	0.441131381745933
1103	76-77	0.4334429602619032
1103	78-79	0.4188392352223502
1103	80-81	0.4156506407167164
1103	82-83	0.43798934282079927
1103	84-85	0.4138333278002335
1103	86-87	0.3626330548062562
1103	88-89	0.38426393503254275
1103	90-91	0.3778112456197569
1103	92-93	0.35078196418484353
1103	94-95	0.37586198267702287
1103	96-97	0.3474765461368321
1103	98-99	0.313873218104046
1103	100	0.36940110310696284
1104	1	0.1898394577052258
1104	2	0.32444156245116673
1104	3	0.23598334059742498
1104	4	0.253000630391675
1104	5	0.20527748279246083
1104	6	0.16415474722460743
1104	7	0.2068537039273579
1104	8	0.2174804524022207
1104	9	0.3486166880935002
1104	10-11	0.31912604624352525
1104	12-13	0.30880403514629506
1104	14-15	0.3805389587511172
1104	16-17	0.4315188288395646
1104	18-19	0.3412394708312547
1104	20-21	0.33149721314434544
1104	22-23	0.3810412040296711
1104	24-25	0.37126968101938473
1104	26-27	0.5052336790377367
1104	28-29	0.5570532836056188
1104	30-31	0.4925119666709179
1104	32-33	0.5540450964943489
1104	34-35	0.5180978593836372
1104	36-37	0.4486475166039554
1104	38-39	0.4573356282372032
1104	40-41	0.4763227592867949
1104	42-43	0.42133040474495687
1104	44-45	0.43334398029707444
1104	46-47	0.44077115359242924
1104	48-49	0.41166500291222974
1104	50-51	0.40999694026934463
1104	52-53	0.3482264491855247
1104	54-55	0.38382027803038454
1104	56-57	0.40486439966657173
1104	58-59	0.4764447044239617
1104	60-61	0.4509448886833738
1104	62-63	0.4653351590383821
1104	64-65	0.47590312163717385
1104	66-67	0.4937932441906909
1104	68-69	0.4582237588445963
1104	70-71	0.3804624829021179
1104	72-73	0.44584645042755255
1104	74-75	0.46097989433373954
1104	76-77	0.38341225208762
1104	78-79	0.43561955437144206
1104	80-81	0.40380132263910795
1104	82-83	0.37220993696999116
1104	84-85	0.3765650298217835
1104	86-87	0.3539960621621212
1104	88-89	0.33849563540473326
1104	90-91	0.3300253229785639
1104	92-93	0.3025105660002829
1104	94-95	0.34575033492257035
1104	96-97	0.39001604542565715
1104	98-99	0.3888175632867146
1104	100	0.3945255027006773
1105	1	0.09389988065843013
1105	2	0.22886185155810423
1105	3	0.23977367813536432
1105	4	0.24910245565389744
1105	5	0.1788185064356611
1105	6	0.2983313524502478
1105	7	0.35307964722553464
1105	8	0.3251284108615238
1105	9	0.3656248532604778
1105	10-11	0.4142097993749445
1105	12-13	0.32619225733976975
1105	14-15	0.43085337805100465
1105	16-17	0.4134914273173891
1105	18-19	0.3978740909400784
1105	20-21	0.39816273389202905
1105	22-23	0.35005280900379177
1105	24-25	0.33356035219182445
1105	26-27	0.382088273854329
1105	28-29	0.4201231961591887
1105	30-31	0.4204603429842706
1105	32-33	0.47976665568224064
1105	34-35	0.46643016651540137
1105	36-37	0.4363138039092007
1105	38-39	0.4279536340584684
1105	40-41	0.36487982286684684
1105	42-43	0.4295726466721064
1105	44-45	0.37376345144484446
1105	46-47	0.304328585111314
1105	48-49	0.2533026243769001
1105	50-51	0.3071791492950666
1105	52-53	0.27527778694159366
1105	54-55	0.3800774888079417
1105	56-57	0.3314231936208998
1105	58-59	0.3547196163913995
1105	60-61	0.2902653100359558
1105	62-63	0.3153340625440251
1105	64-65	0.38805227730723146
1105	66-67	0.3598816556108986
1105	68-69	0.30743916312957253
1105	70-71	0.37223816197627
1105	72-73	0.3522533255460303
1105	74-75	0.34665865106802585
1105	76-77	0.29981850592031734
1105	78-79	0.35747427769205586
1105	80-81	0.3655145494949714
1105	82-83	0.3245516516938629
1105	84-85	0.3232026216316797
1105	86-87	0.3168715730387994
1105	88-89	0.3399729007016674
1105	90-91	0.3295626261559974
1105	92-93	0.3399582372256553
1105	94-95	0.3212220398398671
1105	96-97	0.38299738320149856
1105	98-99	0.3954626943648343
1105	100	0.41607123244641286
1106	1	0.09171063702815019
1106	2	0.09055423920197114
1106	3	0.12125417768226043
1106	4	0.11330746389352697
1106	5	0.03440969629493651
1106	6	0.13267375940247916
1106	7	0.1918994219360357
1106	8	0.1951794339779056
1106	9	0.2941080494580248
1106	10-11	0.31459529866422997
1106	12-13	0.27029532990285077
1106	14-15	0.34478003086837816
1106	16-17	0.2380540906225832
1106	18-19	0.2043668526608613
1106	20-21	0.2515276242957256
1106	22-23	0.3250283714710065
1106	24-25	0.29586196311653623
1106	26-27	0.2393569595753675
1106	28-29	0.35970275614115366
1106	30-31	0.33866685946596675
1106	32-33	0.33106317017747244
1106	34-35	0.22970526754501464
1106	36-37	0.3034638422459892
1106	38-39	0.3637978375139568
1106	40-41	0.33869856678226284
1106	42-43	0.31490627879072974
1106	44-45	0.28825153146373594
1106	46-47	0.250280435960768
1106	48-49	0.23767824780594538
1106	50-51	0.2541125587279396
1106	52-53	0.28135765304382687
1106	54-55	0.3432425987933172
1106	56-57	0.31190926647730777
1106	58-59	0.30899681182369676
1106	60-61	0.30062719245982805
1106	62-63	0.34702228554897374
1106	64-65	0.3897014165596744
1106	66-67	0.41623891508360344
1106	68-69	0.35908466323952837
1106	70-71	0.34408357467593476
1106	72-73	0.3342635949439199
1106	74-75	0.4002555571803441
1106	76-77	0.33601233813012144
1106	78-79	0.34512500533983825
1106	80-81	0.31129462183417544
1106	82-83	0.30515029169635355
1106	84-85	0.34133882151790473
1106	86-87	0.33030746782526776
1106	88-89	0.35702595075309773
1106	90-91	0.35467247014628
1106	92-93	0.3429245547118178
1106	94-95	0.39794034583215065
1106	96-97	0.479349226127745
1106	98-99	0.48191409423700193
1106	100	0.5206574236759458
1107	1	0.18568082099895378
1107	2	0.221944598236
1107	3	0.23993916392208803
1107	4	0.2995114061624875
1107	5	0.2294056454515001
1107	6	0.26303553815260017
1107	7	0.26609381743441674
1107	8	0.24829771405229906
1107	9	0.3346813414351715
1107	10-11	0.3222748933011417
1107	12-13	0.29059141130003496
1107	14-15	0.38385919915590705
1107	16-17	0.32703844599063103
1107	18-19	0.33353086924841335
1107	20-21	0.35962684104986664
1107	22-23	0.36233742605479335
1107	24-25	0.37030530160765807
1107	26-27	0.3923406220704848
1107	28-29	0.43447528172163885
1107	30-31	0.340128916565817
1107	32-33	0.4147624720193335
1107	34-35	0.4043976835740253
1107	36-37	0.32583638626260125
1107	38-39	0.3797612206768264
1107	40-41	0.40714931684723155
1107	42-43	0.43408439018830336
1107	44-45	0.38854261123189104
1107	46-47	0.3217667484430038
1107	48-49	0.3364736169419018
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2216	100	-1.8384956327450475
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	197799.0
3	39235.0
4	25291.0
5	20990.0
6	16315.0
7	15846.0
8	17517.0
9	19047.0
10	19592.0
11	20204.0
12	21815.0
13	22770.0
14	24174.0
15	24806.0
16	24975.0
17	25320.0
18	24608.0
19	24259.0
20	23500.0
21	25970.0
22	31003.0
23	41472.0
24	58682.0
25	73851.0
26	82752.0
27	79352.0
28	87311.0
29	104125.0
30	121465.0
31	142223.0
32	178084.0
33	233100.0
34	317947.0
35	471402.0
36	808882.0
37	1328871.0
38	1638377.0
39	706547.0
40	2097.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.09837132674634	21.538943295013148	14.635806051534505	22.726879326706005
2	15.151067456160238	21.25910715788616	39.73485877017187	23.854966615781738
3	18.273758800690402	27.113359544307166	29.90080208380743	24.712079571195
4	12.38774530507622	18.458951415675102	38.75157823155163	30.40172504769704
5	12.667373969050292	39.780647568195256	34.711457318325	12.840521144429449
6	28.996281018123803	39.25368845250246	18.400837793236036	13.349192736137706
7	25.88824006701764	34.20580253825688	22.461105931179198	17.44485146354627
8	24.216708299759386	37.636469861029774	20.265933655363135	17.880888183847706
9	24.96545332740331	18.4793610588002	21.721622996236974	34.83356261755951
10-11	23.558771351934237	28.519891666072102	28.278226150610376	19.643110831383286
12-13	23.62910343335484	26.336661393163958	28.238938335027182	21.79529683845402
14-15	21.423308525955253	28.381994085243655	25.48182816202346	24.712869226777627
16-17	20.926408833527795	31.491596038512533	26.574976096307097	21.00701903165258
18-19	20.947000507133655	30.4523431948412	28.0025578636839	20.598098434341246
20-21	22.423629374306966	29.153880144298977	27.477601457637295	20.94488902375676
22-23	22.224491560640054	29.443400992215125	27.294437090683253	21.037670356461565
24-25	21.611910067863324	29.465757322592907	27.499762642208125	21.422569967335647
26-27	21.44909550910232	29.668570157856877	27.62482024800419	21.25751408503661
28-29	21.286961867939148	29.60869393201275	28.032272780904943	21.072071419143164
30-31	21.658110609790594	29.746216990564534	27.437299926902426	21.15837247274245
32-33	21.34099327669619	29.567413121617133	27.709577417137787	21.382016184548892
34-35	21.79393141969894	29.2586333119363	27.67606483862042	21.271370429744344
36-37	21.50098479984372	29.44995445344484	27.698845197026483	21.350215549684957
38-39	21.349065281901186	29.529184218143413	27.707023405376486	21.414727094578918
40-41	21.663758638314555	29.0249049522836	27.83226791203098	21.47906849737087
42-43	21.548699903825202	29.33930658923391	27.92647669203508	21.185516814905807
44-45	21.5730081088147	29.206779325890274	27.747614175183276	21.472598390111745
46-47	21.595130658643242	29.12837943904634	27.744262814817176	21.53222708749324
48-49	21.326910488952226	29.164671517944157	27.77471771344089	21.73370027966273
50-51	21.348312561342407	29.215060856847952	27.789718547142968	21.64690803466668
52-53	21.493684367949488	29.18342358732157	27.6901789135018	21.63271313122713
54-55	21.46621151615741	29.208395895523566	27.70879376129664	21.616598827022386
56-57	21.424356527425566	29.17818529601629	27.69546429026526	21.70199388629289
58-59	21.759860542106292	28.840969803120327	27.854534012885768	21.54463564188761
60-61	21.473335696582353	29.270020011066617	27.700144448520113	21.556499843830924
62-63	21.304194066792608	29.16951774074647	27.938994984746856	21.58729320771407
64-65	21.818143627423183	28.991021798202958	27.59649133862237	21.594343235751488
66-67	21.37030790657528	29.027895675338545	27.753572797865715	21.84822362022046
68-69	21.487204854310132	29.24076312623118	27.999487487016513	21.27254453244218
70-71	21.569739752766036	29.170636020338964	27.75699531631437	21.502628910580633
72-73	21.708652957099503	29.1441282930274	27.846703538370043	21.30051521150305
74-75	21.634060585404146	29.05544422439601	27.653756842011962	21.656738348187886
76-77	21.618545140676208	28.77725459730102	27.337347599142127	22.26685266288065
78-79	21.6872650994494	28.756896484123384	27.850096340732527	21.70574207569469
80-81	21.227570055014784	29.120110064571914	27.835617301562575	21.816702578850727
82-83	21.445626284796585	29.236192390300488	27.91360411815441	21.404577206748517
84-85	21.47156938676604	28.6661142982529	27.97775888628637	21.884557428694695
86-87	21.848288167232642	28.942427242013835	27.61000288319584	21.599281707557683
88-89	21.592165282527926	28.955514532109017	27.6722447398422	21.78007544552086
90-91	21.495928900531837	28.772013178693655	27.596161271732594	22.13589664904191
92-93	21.51308591799987	28.84661351820146	27.815230083713523	21.82507048008515
94-95	21.56640374726831	28.681225731789446	27.917364561689485	21.835005959252758
96-97	21.722121710002366	29.0763722426829	27.435688885301087	21.765817162013647
98-99	21.390647311512517	29.110635871214736	27.7314008518611	21.76731596541165
100	21.591156529170426	28.836738217473734	27.564249325653563	22.007855927702277
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17647.0
1	13511.0
2	8554.5
3	6739.5
4	4978.0
5	3839.0
6	3271.0
7	3146.5
8	3310.0
9	3342.5
10	3060.0
11	2663.0
12	2556.5
13	2597.5
14	2760.5
15	3043.0
16	3345.0
17	3931.0
18	4968.5
19	6396.0
20	8541.0
21	11414.0
22	15816.0
23	23136.0
24	32742.5
25	44576.0
26	59633.5
27	76436.5
28	95597.0
29	117820.5
30	137198.5
31	153138.0
32	167042.5
33	178836.5
34	190484.5
35	202563.5
36	212695.5
37	220959.0
38	226354.5
39	230336.0
40	236449.0
41	238614.5
42	236455.0
43	235572.5
44	235038.5
45	237652.0
46	244169.0
47	241377.5
48	237889.0
49	244303.0
50	244847.0
51	230093.0
52	215464.5
53	201114.0
54	192098.0
55	191053.5
56	165975.0
57	134271.5
58	115762.5
59	104715.0
60	89621.0
61	69336.5
62	57852.0
63	48752.0
64	39943.5
65	32712.5
66	26334.5
67	20948.5
68	16961.5
69	13499.5
70	10118.0
71	7510.0
72	5846.5
73	4618.0
74	3642.0
75	2759.5
76	1997.0
77	1612.5
78	1241.5
79	865.0
80	589.5
81	370.0
82	259.0
83	180.5
84	138.0
85	116.5
86	86.0
87	81.5
88	75.5
89	55.5
90	45.0
91	40.0
92	32.0
93	25.5
94	25.5
95	26.0
96	24.0
97	26.0
98	24.5
99	22.0
100	51.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014562611950079367
2	0.008779574704519002
3	0.011216011703859205
4	0.009409687721589744
5	0.01162208453708257
6	0.012770290479300367
7	0.010781933847654915
8	0.008709562147066698
9	0.007169285883115996
10-11	0.007106274581408922
12-13	0.0074003273227086
14-15	0.008716563402811928
16-17	0.00588105482599359
18-19	0.006448156541357258
20-21	0.00602808119664343
22-23	0.006770214305637859
24-25	0.0064131502626311055
26-27	0.006819223095854473
28-29	0.01671199746386512
30-31	0.0061050950098409645
32-33	0.006217115101764652
34-35	0.0056010045961843715
36-37	0.007435333601434753
38-39	0.006147102544312348
40-41	0.005965069894936356
42-43	0.005769034734069903
44-45	0.004690841349304411
46-47	0.006819223095854473
48-49	0.004137742145431204
50-51	0.004326776050552427
52-53	0.0034726228496343103
54-55	0.004879875254425634
56-57	0.0034446178266533886
58-59	0.0029265249015063342
60-61	0.0038856969386029078
62-63	0.003661656754755533
64-65	0.005474981992770223
66-67	0.003556637918577076
68-69	0.004109737122450283
70-71	0.005671017153636676
72-73	0.0033185952232392397
74-75	0.0030665500164109433
76-77	0.002968532435977717
78-79	0.003584642941557998
80-81	0.004389787352259501
82-83	0.0017433126805623856
84-85	0.0028985198785254126
86-87	0.002730489740639881
88-89	0.001841330260995612
90-91	0.0028075035538374163
92-93	0.002758494763620803
94-95	0.0022474030942189792
96-97	0.0014072524047913234
98-99	0.002149385513785753
100	0.0030385449934300216
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7141576.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.67283865324114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.25014173306545	27.191586285431523
2	15.671268833720967	13.061325151984404
3	7.00325722366861	8.755368249872623
4	3.6702655082246807	6.118015293552129
5	2.387704378092976	4.975120964995304
6	1.423528773542191	3.5593490938841983
7	0.9579072168045769	2.7943099023470688
8	0.7025550810689201	2.3421971630719884
9	0.5181663044561482	1.9434114721032563
>10	2.24707286551094	15.647417281453219
>50	0.10107708073178372	2.8796789069686493
>100	0.05628919252694722	4.45624462639326
>500	0.005602426608362298	1.601227345194271
>1k	0.0049608964107915295	3.92430153884456
>5k	1.6873797232509272E-4	0.4527769771194319
>10k+	3.3747594465018546E-5	0.29766974678396935
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21166	0.296377158207096	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	8458	0.11843324218631854	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	7984	0.11179605173984004	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.4002511490460928E-5	0.0
6	0.0	0.0	0.0	1.4002511490460928E-5	0.0
7	0.0	0.0	0.0	5.6010045961843714E-5	0.0
8	0.0	0.0	0.0	5.6010045961843714E-5	0.0
9	0.0	0.0	0.0	5.6010045961843714E-5	0.0
10-11	0.0	0.0	0.0	6.301130170707418E-5	0.0
12-13	0.0	0.0	0.0	7.001255745230465E-5	0.0
14-15	0.0	0.0	0.0	7.001255745230465E-5	0.0
16-17	0.0	0.0	0.0	9.801758043322651E-5	0.0
18-19	0.0	0.0	0.0	2.3104143959260534E-4	0.0
20-21	0.0	0.0	0.0	3.0805525279014044E-4	0.0
22-23	0.0	0.0	0.0	4.690841349304411E-4	0.0
24-25	0.0	0.0	0.0	7.84140643465812E-4	0.0
26-27	0.0	0.0	0.0	0.0012322210111605618	0.0
28-29	0.0	0.0	0.0	0.003115558806627557	0.0
30-31	0.0	0.0	0.0	0.008156462943193492	0.0
32-33	0.0	0.0	0.0	0.017902210940554296	0.0
34-35	0.0	0.0	0.0	0.034635212171655105	0.0
36-37	0.0	0.0	0.0	0.0614290179086521	0.0
38-39	0.0	0.0	0.0	0.0979475678757742	0.0
40-41	0.0	0.0	0.0	0.1337939972913542	0.0
42-43	0.0	0.0	0.0	0.16947939782479385	0.0
44-45	0.0	0.0	0.0	0.21595373346163368	0.0
46-47	0.0	0.0	0.0	0.27475728046582437	0.0
48-49	0.0	0.0	0.0	0.3366693850208974	0.0
50-51	0.0	7.001255745230464E-6	0.0	0.3981614142312565	0.0
52-53	0.0	1.4002511490460928E-5	0.0	0.4587852877292071	0.0
54-55	0.0	1.4002511490460928E-5	0.0	0.5284337798827597	0.0
56-57	0.0	1.4002511490460928E-5	0.0	0.613744081138393	0.0
58-59	0.0	1.4002511490460928E-5	0.0	0.7092202057361008	0.0
60-61	0.0	1.4002511490460928E-5	0.0	0.8100312872116743	0.0
62-63	0.0	1.4002511490460928E-5	0.0	0.9141959701892132	0.0
64-65	0.0	1.4002511490460928E-5	0.0	1.0231145058177633	0.0
66-67	0.0	1.4002511490460928E-5	0.0	1.1541080008110254	0.0
68-69	0.0	1.4002511490460928E-5	0.0	1.305195099793099	0.0
70-71	0.0	1.4002511490460928E-5	0.0	1.4656428777065456	0.0
72-73	0.0	1.4002511490460928E-5	0.0	1.6344641574912875	0.0
74-75	0.0	1.4002511490460928E-5	0.0	1.8097475963288776	0.0
76-77	0.0	1.4002511490460928E-5	0.0	2.0026741996444484	0.0
78-79	0.0	1.4002511490460928E-5	0.0	2.222128560978697	0.0
80-81	0.0	1.4002511490460928E-5	0.0	2.46341983898232	0.0
82-83	0.0	1.4002511490460928E-5	0.0	2.7144288599603223	0.0
84-85	0.0	1.4002511490460928E-5	0.0	2.9688824987649785	0.0
86-87	0.0	1.4002511490460928E-5	0.0	3.2425615858460377	0.0
88	1.4002511490460928E-5	1.4002511490460928E-5	0.0	3.467609950520725	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8185	0.0	43.3657	1
GTATCAA	13915	0.0	34.32643	1
ATCAACG	15000	0.0	30.179398	3
TCAACGC	15095	0.0	29.989046	4
TATCAAC	16690	0.0	27.574135	2
GTGGTAT	4215	0.0	25.207455	1
GTACATG	29720	0.0	24.360716	1
TACATGG	29620	0.0	23.170923	2
TGGTATC	4505	0.0	22.95638	2
ACGCAGA	19530	0.0	22.550444	7
CGCAGAG	19465	0.0	22.263073	8
CAACGCA	20460	0.0	22.148169	5
ACATGGG	30780	0.0	21.793695	3
AACGCAG	21350	0.0	21.222963	6
CATGGGT	6975	0.0	20.218386	4
ATGCGGG	2130	0.0	19.418322	8
TACACGC	3460	0.0	19.020344	5
GCAGAGT	21945	0.0	18.80475	9
TTACACG	3635	0.0	18.492733	4
CATGGGG	18355	0.0	17.773565	4
>>END_MODULE