FastQCFastQC Report
Wed 25 May 2016
SRR2431444_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431444_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7141576
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT406050.568571979070166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351080.4916001734071023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248550.3480324230954064No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88270.12360016892629862No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82680.11577276500313097No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA73810.10335253731109213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154700.059.1959341
GTATCAA293200.041.6337361
GTGGTAT52750.037.111651
TGGTATC55950.034.3834532
ATCAACG373950.032.3381233
TCAACGC379400.031.9229624
TATCAAC380250.031.8394572
CAACGCA423200.028.61475
AACGCAG437500.027.6983786
ACGCAGA462350.026.0673337
CGCAGAG467050.025.704378
GCAGAGT497650.023.840479
GTACATG283600.023.429761
TACATGG286900.022.4275512
GAGTACT338950.021.8097812-13
ACATGGG291000.021.497353
AGAGTAC479600.020.75851410-11
CAGAGTA496300.020.72299410-11
AGTACTT347500.020.4955412-13
GTACTTT362450.020.29846614-15
Produced by FastQC (version 0.11.5)