##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431444_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7141576
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29266537246121	33.0	31.0	34.0	30.0	34.0
2	32.43268712676306	34.0	31.0	34.0	31.0	34.0
3	32.5258186708368	34.0	31.0	34.0	31.0	34.0
4	36.06890663909479	37.0	35.0	37.0	35.0	37.0
5	35.97268236030814	37.0	35.0	37.0	35.0	37.0
6	36.010195788716665	37.0	35.0	37.0	35.0	37.0
7	35.95643062539697	37.0	35.0	37.0	35.0	37.0
8	35.940477704081005	37.0	35.0	37.0	35.0	37.0
9	37.635288485342734	39.0	37.0	39.0	35.0	39.0
10-11	37.57097579021773	39.0	37.0	39.0	35.0	39.0
12-13	37.54603178626118	39.0	37.0	39.0	35.0	39.0
14-15	38.908712796727215	40.0	38.0	41.0	35.0	41.0
16-17	38.959025920889175	40.0	38.0	41.0	35.0	41.0
18-19	39.023615095603546	40.5	38.5	41.0	35.0	41.0
20-21	39.04507128398549	41.0	39.0	41.0	35.0	41.0
22-23	39.005998745935074	41.0	39.0	41.0	35.0	41.0
24-25	38.953305824932755	41.0	38.5	41.0	35.0	41.0
26-27	38.81795971925524	40.0	38.0	41.0	35.0	41.0
28-29	38.73810472646374	40.0	38.0	41.0	34.5	41.0
30-31	38.58698339134107	40.0	38.0	41.0	34.0	41.0
32-33	38.424305993523	40.0	38.0	41.0	34.0	41.0
34-35	38.30091460204302	40.0	38.0	41.0	34.0	41.0
36-37	38.17286226457577	40.0	38.0	41.0	34.0	41.0
38-39	38.07829343271009	40.0	38.0	41.0	33.0	41.0
40-41	37.96112398719835	40.0	37.0	41.0	33.0	41.0
42-43	37.84763762228393	40.0	37.0	41.0	33.0	41.0
44-45	37.6480114473332	40.0	37.0	41.0	33.0	41.0
46-47	37.37064934966736	40.0	37.0	41.0	32.0	41.0
48-49	37.149199700458276	40.0	37.0	41.0	32.0	41.0
50-51	37.00766014392342	40.0	36.5	41.0	31.0	41.0
52-53	36.850202182263416	40.0	36.0	41.0	31.0	41.0
54-55	36.68536559717351	40.0	35.5	41.0	31.0	41.0
56-57	36.44657026684306	39.0	35.0	41.0	31.0	41.0
58-59	36.26216566203314	39.0	35.0	41.0	30.0	41.0
60-61	36.05782701185285	39.0	35.0	41.0	30.0	41.0
62-63	35.84679572128057	38.5	35.0	41.0	30.0	41.0
64-65	35.61600051585252	38.0	35.0	41.0	29.5	41.0
66-67	35.36637081226889	37.0	35.0	40.0	29.0	41.0
68-69	35.09445079069382	37.0	35.0	40.0	29.0	41.0
70-71	34.786400858858045	36.5	34.5	39.5	29.0	41.0
72-73	34.47968543637987	36.0	34.0	39.0	29.0	41.0
74-75	34.17927744520257	35.5	34.0	39.0	28.5	41.0
76-77	33.84084185339482	35.0	34.0	38.5	28.0	40.0
78-79	33.537873292953826	35.0	34.0	37.0	28.0	39.0
80-81	33.24241560966375	35.0	34.0	37.0	27.0	39.0
82-83	33.00238994866119	35.0	34.0	36.0	27.5	39.0
84-85	32.784428115026714	35.0	34.0	36.0	27.0	37.0
86-87	32.549966702027675	35.0	34.0	36.0	27.0	37.0
88-89	32.41095802943216	35.0	34.0	35.0	27.0	37.0
90-91	32.23002317695702	35.0	34.0	35.0	27.0	36.0
92-93	32.047904762198144	35.0	33.5	35.0	26.5	36.0
94-95	31.895520260513926	35.0	33.0	35.0	26.0	36.0
96-97	31.74560825229613	35.0	33.0	35.0	26.0	36.0
98-99	31.58984305425021	35.0	33.0	35.0	25.5	35.5
100	31.485227070327333	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1719876390024737
1101	2	0.2392571590329453
1101	3	0.20384660706437074
1101	4	0.16506016025996217
1101	5	0.15691957250880506
1101	6	0.2093906390208815
1101	7	0.17883081082144514
1101	8	0.26218780034682965
1101	9	0.34181372725475256
1101	10-11	0.3339863697504768
1101	12-13	0.354452824350858
1101	14-15	0.45293885039863824
1101	16-17	0.4435204175568046
1101	18-19	0.4201134314548227
1101	20-21	0.38499335657792244
1101	22-23	0.38728613569376336
1101	24-25	0.34486817404870607
1101	26-27	0.3725894323633838
1101	28-29	0.38628111061031944
1101	30-31	0.44181092577218806
1101	32-33	0.45543580266122774
1101	34-35	0.444566298232715
1101	36-37	0.4653163797580504
1101	38-39	0.42882662752269596
1101	40-41	0.5241422080932026
1101	42-43	0.5479175763205504
1101	44-45	0.6106434067863518
1101	46-47	0.5994685276628715
1101	48-49	0.5560988727445206
1101	50-51	0.5370265326321189
1101	52-53	0.5645074899716604
1101	54-55	0.5469290983559958
1101	56-57	0.5584620894454702
1101	58-59	0.6011582901021058
1101	60-61	0.6239534869539511
1101	62-63	0.6302653081897347
1101	64-65	0.5956314047829565
1101	66-67	0.5590569444437037
1101	68-69	0.48461018293273384
1101	70-71	0.5567013637357618
1101	72-73	0.5545994130400587
1101	74-75	0.5510849670152638
1101	76-77	0.6157111095758836
1101	78-79	0.5654819459803093
1101	80-81	0.5969140734353573
1101	82-83	0.5670215212364198
1101	84-85	0.5274980753033915
1101	86-87	0.5297100405922777
1101	88-89	0.5185930513467127
1101	90-91	0.5611400492232974
1101	92-93	0.562767071922309
1101	94-95	0.5604852890377074
1101	96-97	0.6117131958054607
1101	98-99	0.7005907206091635
1101	100	0.6792800210245638
1102	1	0.13180429410997618
1102	2	0.19816092401858043
1102	3	0.18398466314284434
1102	4	0.1282970273956039
1102	5	0.15408024822465194
1102	6	0.09724389375188736
1102	7	0.11956325905288878
1102	8	0.15930186950367187
1102	9	0.22235581583099417
1102	10-11	0.24670946991891185
1102	12-13	0.23498598594800768
1102	14-15	0.3503397123821941
1102	16-17	0.30904480079270513
1102	18-19	0.35456017672382245
1102	20-21	0.296717427716338
1102	22-23	0.2734381866201474
1102	24-25	0.27259199749069296
1102	26-27	0.2878648262006962
1102	28-29	0.297989527334785
1102	30-31	0.28393024609319895
1102	32-33	0.2582406376954083
1102	34-35	0.333975398599307
1102	36-37	0.287270476656893
1102	38-39	0.2746037552349705
1102	40-41	0.3183440410467355
1102	42-43	0.33819707201063665
1102	44-45	0.3377212676800383
1102	46-47	0.305524502100603
1102	48-49	0.29897966636386286
1102	50-51	0.2635571479100278
1102	52-53	0.254937733704935
1102	54-55	0.24925065289875192
1102	56-57	0.3474644326536236
1102	58-59	0.26382199266824813
1102	60-61	0.23457631961024106
1102	62-63	0.22512333850777821
1102	64-65	0.26352701064841
1102	66-67	0.27820823741903666
1102	68-69	0.28939773866443375
1102	70-71	0.28992646529129473
1102	72-73	0.26051935755605626
1102	74-75	0.3105955882378879
1102	76-77	0.29140893480128227
1102	78-79	0.24512551082723633
1102	80-81	0.32966221075825075
1102	82-83	0.23533479776470756
1102	84-85	0.23715324225077694
1102	86-87	0.21811024865726836
1102	88-89	0.2506423330270877
1102	90-91	0.24079834209954498
1102	92-93	0.23873047229880484
1102	94-95	0.3055565661604689
1102	96-97	0.3062251298191363
1102	98-99	0.3842690669878408
1102	100	0.3822784159517383
1103	1	0.1123842978991334
1103	2	0.1610880059637907
1103	3	0.1285828930006616
1103	4	0.12968898698878917
1103	5	0.10327877996801504
1103	6	0.12221977655001837
1103	7	0.12434627313690072
1103	8	0.12312074517977578
1103	9	0.20575832709935327
1103	10-11	0.22013885615295692
1103	12-13	0.2137832827813142
1103	14-15	0.2980240096838003
1103	16-17	0.2834706118069903
1103	18-19	0.2545551563965418
1103	20-21	0.2463177155522871
1103	22-23	0.21983574719337184
1103	24-25	0.21042291375344035
1103	26-27	0.2362381891621439
1103	28-29	0.2122542652334758
1103	30-31	0.17324076260202048
1103	32-33	0.23097022379565146
1103	34-35	0.22180946062587736
1103	36-37	0.249630132671804
1103	38-39	0.195271421617484
1103	40-41	0.2675974528484417
1103	42-43	0.27955009380307416
1103	44-45	0.3023871130800586
1103	46-47	0.2868123893567329
1103	48-49	0.227274202969852
1103	50-51	0.20345932206490858
1103	52-53	0.24988398569816184
1103	54-55	0.28807171684862
1103	56-57	0.3370874585208341
1103	58-59	0.3243286475224707
1103	60-61	0.3170061287565886
1103	62-63	0.3434423866668155
1103	64-65	0.3269236736252239
1103	66-67	0.33286891025566945
1103	68-69	0.2868690795916251
1103	70-71	0.3353230032073995
1103	72-73	0.3093779678186124
1103	74-75	0.32842280695309967
1103	76-77	0.33431899613376004
1103	78-79	0.33027021326857664
1103	80-81	0.2916285644998453
1103	82-83	0.30602354143844224
1103	84-85	0.29323148633680063
1103	86-87	0.2986822239644624
1103	88-89	0.3136556776093471
1103	90-91	0.3084149909482363
1103	92-93	0.3154981519533848
1103	94-95	0.35571613596855656
1103	96-97	0.35162038306264876
1103	98-99	0.3956839218523669
1103	100	0.4146803318248722
1104	1	0.11925375247217573
1104	2	0.18110042418153682
1104	3	0.1655431720971805
1104	4	0.14978571024066412
1104	5	0.1308157129951013
1104	6	0.1487199713250149
1104	7	0.17809603660299445
1104	8	0.16174013535261622
1104	9	0.26000481177810286
1104	10-11	0.27052235971574135
1104	12-13	0.2608767533358076
1104	14-15	0.3727942344202546
1104	16-17	0.3024344275915354
1104	18-19	0.27519911265898855
1104	20-21	0.2879367568094935
1104	22-23	0.27279966328929106
1104	24-25	0.26517494749525383
1104	26-27	0.2497260012557021
1104	28-29	0.273450292393413
1104	30-31	0.2811475139620683
1104	32-33	0.23626382273069169
1104	34-35	0.2636810608726279
1104	36-37	0.2706694866665771
1104	38-39	0.2593394990008946
1104	40-41	0.22783062955209488
1104	42-43	0.18256159407432193
1104	44-45	0.18809456464046548
1104	46-47	0.19511563302759072
1104	48-49	0.16087075618720803
1104	50-51	0.18802788346190624
1104	52-53	0.13832383501218715
1104	54-55	0.12041854258602314
1104	56-57	0.1905854202057924
1104	58-59	0.23075429550543447
1104	60-61	0.15170869729744396
1104	62-63	0.10875149113068261
1104	64-65	0.12989248158728373
1104	66-67	0.12805655849233233
1104	68-69	0.15859111693504957
1104	70-71	0.15990964239265537
1104	72-73	0.1544932764132767
1104	74-75	0.15475764165825723
1104	76-77	0.19710981663878613
1104	78-79	0.15469364030682442
1104	80-81	0.14924701438479815
1104	82-83	0.18834467831862867
1104	84-85	0.23527121308802634
1104	86-87	0.1979759996814323
1104	88-89	0.18150729295227563
1104	90-91	0.1923365177682541
1104	92-93	0.21817162614847518
1104	94-95	0.17539567112957144
1104	96-97	0.20645601710997497
1104	98-99	0.22895924627493258
1104	100	0.250879249121823
1105	1	0.06100993078771211
1105	2	0.13719832769164242
1105	3	0.1086065767096045
1105	4	0.10695845280407212
1105	5	0.08940238411950929
1105	6	0.10525599663803575
1105	7	0.11566864045529002
1105	8	0.12243976392110056
1105	9	0.14172287162963215
1105	10-11	0.15577440807336984
1105	12-13	0.109472061687228
1105	14-15	0.20629007407535482
1105	16-17	0.15857395578484557
1105	18-19	0.18443462287959278
1105	20-21	0.1742789475473785
1105	22-23	0.14274611482485966
1105	24-25	0.13775241802517968
1105	26-27	0.21915912502132784
1105	28-29	0.17161564864864687
1105	30-31	0.17324217221498373
1105	32-33	0.20032147900347752
1105	34-35	0.18607632479314162
1105	36-37	0.2183892122376605
1105	38-39	0.1990802511235259
1105	40-41	0.1835802084726339
1105	42-43	0.21290707546682341
1105	44-45	0.26527382795880783
1105	46-47	0.3038061580935292
1105	48-49	0.37524323814008653
1105	50-51	0.3428232505224571
1105	52-53	0.31474845942717167
1105	54-55	0.2974248581738692
1105	56-57	0.33168833683684795
1105	58-59	0.2973484342872723
1105	60-61	0.27290790736753223
1105	62-63	0.28130471247735045
1105	64-65	0.3139033550770236
1105	66-67	0.3183944515581061
1105	68-69	0.2991458687608457
1105	70-71	0.32860605923946196
1105	72-73	0.27594261167409684
1105	74-75	0.28115950695075753
1105	76-77	0.342906717619897
1105	78-79	0.2674260742292205
1105	80-81	0.2694148607622395
1105	82-83	0.25628490579342866
1105	84-85	0.2580162312510268
1105	86-87	0.2588667660713071
1105	88-89	0.21625379883296603
1105	90-91	0.25070162197988566
1105	92-93	0.23585836389726467
1105	94-95	0.25218075479373425
1105	96-97	0.24863325841218042
1105	98-99	0.28646547871958106
1105	100	0.29437992616590236
1106	1	0.04426089264460842
1106	2	0.12131068910392173
1106	3	0.06280910239496507
1106	4	0.09176292454834822
1106	5	0.09842510108557434
1106	6	0.09937125164695715
1106	7	0.08755378429921734
1106	8	0.11800576822131603
1106	9	0.12982108623869237
1106	10-11	0.14938354942921706
1106	12-13	0.1324774041043142
1106	14-15	0.18993888775388257
1106	16-17	0.15844436227103387
1106	18-19	0.20333868360275176
1106	20-21	0.14651873946922223
1106	22-23	0.15418970311998237
1106	24-25	0.1775495151021218
1106	26-27	0.20779673531445297
1106	28-29	0.17093424845118932
1106	30-31	0.20004092203666346
1106	32-33	0.1388888471139751
1106	34-35	0.20173979468397363
1106	36-37	0.20966464273899277
1106	38-39	0.22784827488914772
1106	40-41	0.2190039563188293
1106	42-43	0.22424538730299304
1106	44-45	0.24495407170977046
1106	46-47	0.2526540151488561
1106	48-49	0.27739534267354315
1106	50-51	0.2792441269541186
1106	52-53	0.268025625944766
1106	54-55	0.25699037964101734
1106	56-57	0.32057559336776364
1106	58-59	0.3096426942858983
1106	60-61	0.31967491018890826
1106	62-63	0.2670198468524134
1106	64-65	0.2845709453134049
1106	66-67	0.3369859694717192
1106	68-69	0.3748478370231396
1106	70-71	0.3241666420323597
1106	72-73	0.34023632002548965
1106	74-75	0.3417955759058131
1106	76-77	0.33632573415563627
1106	78-79	0.29472456831875604
1106	80-81	0.30124113731282876
1106	82-83	0.2639453538849139
1106	84-85	0.22238765318908094
1106	86-87	0.2166594989411763
1106	88-89	0.21872476595261503
1106	90-91	0.2554181030148541
1106	92-93	0.26476759489578683
1106	94-95	0.2714627264676679
1106	96-97	0.26407836203631163
1106	98-99	0.2640849118680393
1106	100	0.3368366889364047
1107	1	0.08056558506822853
1107	2	0.11162668249064467
1107	3	0.07810098460408454
1107	4	0.10934176351079827
1107	5	0.07613911443714017
1107	6	0.0689167195528384
1107	7	0.02668725707190589
1107	8	0.029183625984067874
1107	9	0.057520672212270085
1107	10-11	0.07084848648486997
1107	12-13	0.030680678063454536
1107	14-15	0.15275344219767817
1107	16-17	0.1376365608889074
1107	18-19	0.126930572201033
1107	20-21	0.10735166836218468
1107	22-23	0.11212261519136035
1107	24-25	0.13845459813440897
1107	26-27	0.1628758933903498
1107	28-29	0.1409567478471203
1107	30-31	0.133995890677987
1107	32-33	0.12559125149758188
1107	34-35	0.17157920920427472
1107	36-37	0.17470400443481537
1107	38-39	0.1411563098278279
1107	40-41	0.1252156842660881
1107	42-43	0.13388139235306795
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2216	72-73	-0.8151351654804628
2216	74-75	-0.7896597450352019
2216	76-77	-0.7251878131733633
2216	78-79	-0.6918206263142679
2216	80-81	-0.7149956463975258
2216	82-83	-0.7590451536222673
2216	84-85	-0.7720832796568828
2216	86-87	-0.7885821933718411
2216	88-89	-0.7679092342271332
2216	90-91	-0.7898901916221206
2216	92-93	-0.7592234744854878
2216	94-95	-0.8554370833715623
2216	96-97	-1.0458901424430884
2216	98-99	-1.054120555707705
2216	100	-1.0743271551858093
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	205.0
3	721.0
4	533.0
5	432.0
6	644.0
7	1349.0
8	2755.0
9	4520.0
10	6550.0
11	8117.0
12	10529.0
13	14360.0
14	17798.0
15	24241.0
16	31460.0
17	44788.0
18	50234.0
19	29428.0
20	20464.0
21	19433.0
22	20383.0
23	21740.0
24	25290.0
25	31064.0
26	40001.0
27	52008.0
28	67775.0
29	85905.0
30	109938.0
31	140364.0
32	177190.0
33	222714.0
34	294618.0
35	437201.0
36	738026.0
37	1413718.0
38	1948785.0
39	1019784.0
40	6511.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.228279720828326	21.8481114445257	15.248968235956662	24.674640598689315
2	13.674348631170485	21.41654727191869	39.497836331924496	25.411267764986327
3	16.29403099602888	26.950176151989204	31.23076750737934	25.525025344602582
4	11.358290616398987	18.57056540490217	38.695447104846984	31.375696873851854
5	12.058685721471356	38.60401561300539	35.51575037675603	13.821548288767223
6	28.757041863028554	39.2432986780509	17.868408877816325	14.131250581104226
7	25.347976413049444	33.95812072853387	22.577383479500885	18.1165193789158
8	25.09300468131964	36.00782236301903	19.903267850121598	18.99590510553973
9	25.32204376176911	16.585792827801594	22.021105705519343	36.071057704909954
10-11	23.867949167697724	27.346498644747353	28.36616258690457	20.419389600650355
12-13	24.54486236651406	25.235200185505274	28.888105370579265	21.3318320774014
14-15	21.813077393561308	26.992186318538092	26.965273491453427	24.22946279644717
16-17	20.248555780964875	30.22533821666254	27.990999465664164	21.535106536708422
18-19	20.17318726286747	28.12578064001559	31.26149606193367	20.43953603518327
20-21	21.401564584623898	27.098262344334078	31.120427759923018	20.379745311119002
22-23	21.17492693489504	27.27147341147108	31.08124173151697	20.47235792211691
24-25	20.87295536189998	27.19468266320523	31.094693095121574	20.837668879773215
26-27	20.651905125703347	27.48838631696981	31.159207715495853	20.70050084183099
28-29	20.58924141871063	27.415513848174523	31.547030023573296	20.44821470954155
30-31	20.958979773833082	27.52247361604163	31.011213125419346	20.507333484705942
32-33	20.527717774966227	27.50491541523085	31.297901362915837	20.669465446887088
34-35	20.97212389182262	27.314268967000565	31.022126300295067	20.69148084088175
36-37	20.68783332544494	27.500513212311382	30.924416499306705	20.88723696293697
38-39	20.717582852281915	27.505837379532938	30.936998309172825	20.83958145901232
40-41	20.875170053476836	27.21011058805754	30.947047775023844	20.967671583441778
42-43	20.847579107058927	27.53588891772681	30.917578240987766	20.698953734226492
44-45	21.024052817480737	27.396120603648356	30.62024133331933	20.959585245551576
46-47	21.06590748491031	27.431319692080784	30.25819260346688	21.244580219542023
48-49	20.965784961166626	27.54405283567769	29.86516349839488	21.624998704760795
50-51	21.152281618367994	27.623221898696993	29.649836602176638	21.574659880758375
52-53	21.21354205465618	27.81868723671557	29.482598652723034	21.485172055905217
54-55	21.246237399976433	27.94479790637953	29.429961151743306	21.37900354190073
56-57	21.301870022768064	27.775812639517234	29.4225938340683	21.499723503646408
58-59	21.505408040761015	27.37190378690988	29.640001226729083	21.482686945600026
60-61	21.134329904774706	27.847772557108218	29.473589876615296	21.544307661501776
62-63	21.092053684726718	27.484814597926068	29.809072397309322	21.6140593200379
64-65	21.202540419391003	27.587846415415925	29.62247556465044	21.58713760054263
66-67	21.107926080944264	27.537963123927188	29.65641231334545	21.6976984817831
68-69	21.32079426777573	27.60571592522097	29.653606003539025	21.419883803464277
70-71	21.282281085756424	27.69440764685912	29.448712544264747	21.574598723119706
72-73	21.34929320923001	27.55069049184662	29.529056331543625	21.57095996737975
74-75	21.345706909377324	27.557946877871615	29.182052246969764	21.914293965781297
76-77	21.403111862143597	27.27480600920581	29.06577623762598	22.256305891024613
78-79	21.34909371724706	27.35245293903902	29.406024055052587	21.89242928866133
80-81	20.807767081103666	27.61671233352414	29.48611763005813	22.089402955314068
82-83	21.05689207533632	27.72510323642996	29.545422243842346	21.672582444391374
84-85	21.113868610992302	27.334885427618595	29.45213377490983	22.099112186479278
86-87	21.23753916502464	27.53272526960436	29.179602653531937	22.050132911839068
88-89	21.114142032514952	27.516566371344364	29.171096127801484	22.1981954683392
90-91	21.006721765615882	27.481413066247562	29.05106659930525	22.460798568831304
92-93	21.001346904409274	27.575252805374063	29.29533193093219	22.128068359284477
94-95	20.913934123224344	27.41032231541049	29.329933616893527	22.34580994447164
96-97	20.981462419837047	27.824307534487975	28.883433799427493	22.310796246247484
98-99	20.630379574373226	27.7529624744024	29.26831522367344	22.348342727550932
100	20.862722957520013	27.560819771503418	29.141974901530492	22.434482369446076
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2086.0
1	1328.0
2	1638.0
3	2950.0
4	2088.5
5	1459.0
6	4426.0
7	12128.0
8	20331.0
9	22078.5
10	15054.0
11	7631.0
12	6006.0
13	6300.5
14	6796.0
15	7313.0
16	7913.0
17	8753.0
18	9896.0
19	11019.5
20	12808.0
21	15402.5
22	19303.0
23	26123.0
24	35060.0
25	46129.5
26	60560.5
27	76430.0
28	95032.0
29	117000.0
30	135590.0
31	151133.0
32	165337.0
33	177135.0
34	188918.0
35	201122.5
36	211082.0
37	219477.5
38	225411.0
39	229823.0
40	236358.5
41	238882.5
42	236804.5
43	238283.5
44	241771.5
45	244129.5
46	244433.0
47	240920.5
48	235819.0
49	239951.0
50	247078.5
51	235286.0
52	214522.0
53	197081.0
54	186990.5
55	179643.5
56	153750.5
57	123707.0
58	108821.0
59	105103.0
60	90219.5
61	66650.5
62	54923.5
63	46014.5
64	37467.5
65	30399.5
66	24107.5
67	19004.0
68	15083.0
69	11828.0
70	8721.0
71	6042.5
72	4555.0
73	3620.0
74	2662.0
75	1854.0
76	1328.5
77	1055.5
78	764.5
79	470.0
80	255.5
81	97.5
82	44.0
83	19.0
84	8.0
85	3.0
86	1.5
87	1.5
88	2.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.5
94	2.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04643232810236844
2	0.0
3	2.2404018384737488E-4
4	0.07088071316471323
5	0.004900879021661325
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.011776112163477641
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	4.340778562042888E-4
26-27	0.0
28-29	2.940527412996795E-4
30-31	0.0036056467087936892
32-33	1.750313936307616E-4
34-35	0.0016803013788553116
36-37	0.003948708240309982
38-39	0.001414253660536554
40-41	0.0010151820830584175
42-43	0.0018273277495051514
44-45	1.400251149046093E-5
46-47	3.850690659876756E-4
48-49	5.320954366375153E-4
50-51	0.0015402762639507024
52-53	8.401506894276558E-4
54-55	0.0010011795715679564
56-57	0.006063087475369582
58-59	0.00889159479644269
60-61	0.0012742285456319444
62-63	0.002492447045302045
64-65	0.0010921958962559525
66-67	1.0501883617845698E-4
68-69	7.701381319753512E-4
70-71	4.1307408896859745E-4
72-73	0.0
74-75	1.8903390512122254E-4
76-77	0.0
78-79	5.741029711088981E-4
80-81	0.0
82-83	0.0011272021749821049
84-85	5.951067383445894E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.1003767235691395E-4
94-95	0.0
96-97	4.0607283322336693E-4
98-99	5.250941808922848E-4
100	3.500627872615232E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7141576.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.27772818162872
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.14172613993972	21.455274650970587
2	18.106016825964755	13.136903137286907
3	8.291560880811966	9.023969755065671
4	4.410261275531272	6.399770390547466
5	2.6003489184566013	4.716737562058039
6	1.7621041614595478	3.835508147828777
7	1.1683745890709982	2.967018302964788
8	0.8662104469437705	2.5139317713850584
9	0.6448590357619743	2.1054618733356083
>10	2.7947927762123426	17.038829179240405
>50	0.12631089473126572	3.1637196836266694
>100	0.07346177744939497	5.154651998776506
>500	0.006889493280945226	1.7523142806161591
>1k	0.006618339508561659	4.492745066419406
>5k	3.483336578210669E-4	0.8311052536252358
>10k+	1.1611121927368896E-4	1.41205894625258
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40605	0.568571979070166	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35108	0.4916001734071023	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24855	0.3480324230954064	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8827	0.12360016892629862	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8268	0.11577276500313097	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	7381	0.10335253731109213	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4002511490460928E-5	0.0
2	0.0	0.0	0.0	1.4002511490460928E-5	0.0
3	0.0	0.0	0.0	1.4002511490460928E-5	0.0
4	0.0	0.0	0.0	1.4002511490460928E-5	0.0
5	0.0	0.0	0.0	2.8005022980921857E-5	0.0
6	0.0	0.0	0.0	2.8005022980921857E-5	0.0
7	0.0	0.0	0.0	2.8005022980921857E-5	0.0
8	0.0	0.0	0.0	2.8005022980921857E-5	0.0
9	0.0	0.0	0.0	2.8005022980921857E-5	0.0
10-11	0.0	0.0	0.0	5.6010045961843714E-5	0.0
12-13	0.0	0.0	0.0	8.401506894276557E-5	0.0
14-15	0.0	0.0	0.0	8.401506894276557E-5	0.0
16-17	0.0	0.0	0.0	1.1202009192368744E-4	0.0
18-19	0.0	0.0	0.0	1.96035160866453E-4	0.0
20-21	0.0	0.0	0.0	2.590464625735272E-4	0.0
22-23	0.0	0.0	0.0	4.4808036769474976E-4	0.0
24-25	0.0	0.0	0.0	8.261481779371948E-4	0.0
26-27	0.0	0.0	0.0	0.0012742285456319447	0.0
28-29	0.0	0.0	0.0	0.003213576387060783	0.0
30-31	0.0	0.0	0.0	0.008268483035117178	0.0
32-33	0.0	0.0	0.0	0.01825927498356105	0.0
34-35	0.0	0.0	0.0	0.035321335234687695	0.0
36-37	0.0	0.0	0.0	0.06246520375894621	0.0
38-39	0.0	0.0	0.0	0.09957185920866768	0.0
40-41	0.0	0.0	0.0	0.13589437401492332	0.0
42-43	0.0	0.0	0.0	0.17214687626372666	0.0
44-45	0.0	0.0	0.0	0.21927232868487292	0.0
46-47	0.0	0.0	0.0	0.2790630527491411	0.0
48-49	0.0	0.0	0.0	0.3419553331085463	0.0
50-51	0.0	0.0	0.0	0.404161490404919	0.0
52-53	0.0	0.0	0.0	0.4659475723565779	0.0
54-55	1.4002511490460928E-5	0.0	0.0	0.5368352867770363	0.0
56-57	1.4002511490460928E-5	0.0	0.0	0.623769879365563	0.0
58-59	1.4002511490460928E-5	0.0	0.0	0.7209333065978714	0.0
60-61	1.4002511490460928E-5	0.0	0.0	0.8239147773544663	0.0
62-63	1.4002511490460928E-5	0.0	0.0	0.9300748182193959	0.0
64-65	1.4002511490460928E-5	0.0	0.0	1.0408556878761774	0.0
66-67	1.4002511490460928E-5	0.0	0.0	1.1740335746619515	0.0
68-69	1.4002511490460928E-5	0.0	0.0	1.3268079762786253	0.0
70-71	1.4002511490460928E-5	0.0	0.0	1.4889990668726343	0.0
72-73	1.4002511490460928E-5	0.0	0.0	1.660263784912462	0.0
74-75	1.4002511490460928E-5	0.0	0.0	1.8382497084677107	0.0
76-77	1.4002511490460928E-5	0.0	0.0	2.0332766885068505	0.0
78-79	1.4002511490460928E-5	0.0	0.0	2.2558046011132555	0.0
80-81	1.4002511490460928E-5	0.0	0.0	2.5007645371273792	0.0
82-83	1.4002511490460928E-5	0.0	0.0	2.7559463065295393	0.0
84-85	1.4002511490460928E-5	0.0	0.0	3.01525181556564	0.0
86-87	1.4002511490460928E-5	0.0	0.0	3.295071003935266	0.0
88	1.4002511490460928E-5	0.0	0.0	3.524040071827283	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15470	0.0	59.195934	1
GTATCAA	29320	0.0	41.633736	1
GTGGTAT	5275	0.0	37.11165	1
TGGTATC	5595	0.0	34.383453	2
ATCAACG	37395	0.0	32.338123	3
TCAACGC	37940	0.0	31.922962	4
TATCAAC	38025	0.0	31.839457	2
CAACGCA	42320	0.0	28.6147	5
AACGCAG	43750	0.0	27.698378	6
ACGCAGA	46235	0.0	26.067333	7
CGCAGAG	46705	0.0	25.70437	8
GCAGAGT	49765	0.0	23.84047	9
GTACATG	28360	0.0	23.42976	1
TACATGG	28690	0.0	22.427551	2
GAGTACT	33895	0.0	21.80978	12-13
ACATGGG	29100	0.0	21.49735	3
AGAGTAC	47960	0.0	20.758514	10-11
CAGAGTA	49630	0.0	20.722994	10-11
AGTACTT	34750	0.0	20.49554	12-13
GTACTTT	36245	0.0	20.298466	14-15
>>END_MODULE