FastQCFastQC Report
Wed 25 May 2016
SRR2431443_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431443_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7283159
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA366480.5031882456499989No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99000.046.0112151
GTATCAA169050.034.536761
TCAACGC183250.030.884234
ATCAACG186350.030.471153
TATCAAC200200.028.6448822
CAACGCA208150.027.1896955
GTGGTAT50050.026.110541
AACGCAG220700.025.5986846
GTACATG314900.025.168661
CATGGGT154400.025.0861594
TACATGG314700.024.3020042
CGCAGAG232700.023.6314328
ACGCAGA237650.023.4953637
ACATGGG323150.022.9537733
TGGTATC54600.022.4697952
GCAGAGT256000.020.3790329
ATGGGTG91400.020.005885
ATGGGTA68500.019.5572835
CAGAGTA249550.018.39116710-11
TACCTGG50900.017.2693162
Produced by FastQC (version 0.11.5)