##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431443_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7283159
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.33799014960404	31.0	31.0	34.0	26.0	34.0
2	30.330853960486102	31.0	31.0	34.0	26.0	34.0
3	30.43417190260435	31.0	31.0	34.0	26.0	34.0
4	33.75182170264304	37.0	35.0	37.0	28.0	37.0
5	33.913206480869086	37.0	35.0	37.0	30.0	37.0
6	34.00903385467762	37.0	35.0	37.0	30.0	37.0
7	33.90313186901453	37.0	35.0	37.0	29.0	37.0
8	33.84358572976369	37.0	35.0	37.0	29.0	37.0
9	35.21641090081928	39.0	37.0	39.0	28.0	39.0
10-11	35.164290934744116	39.0	36.0	39.0	27.5	39.0
12-13	35.13741640131707	39.0	35.0	39.0	27.0	39.0
14-15	36.3590215454585	40.0	37.0	41.0	27.0	41.0
16-17	36.381272398419426	40.0	37.0	41.0	27.0	41.0
18-19	36.386554241092355	40.0	37.0	41.0	27.0	41.0
20-21	36.49326858029599	40.0	37.0	41.0	28.0	41.0
22-23	36.462596587552184	40.0	37.0	41.0	28.0	41.0
24-25	36.50006171772441	40.0	37.0	41.0	28.0	41.0
26-27	36.49489816163563	40.0	37.0	41.0	29.0	41.0
28-29	36.386700194242636	40.0	36.0	41.0	28.5	41.0
30-31	36.36845426551858	40.0	36.0	41.0	29.0	41.0
32-33	36.31176094054791	40.0	36.0	41.0	29.0	41.0
34-35	36.21150355223605	40.0	36.0	41.0	28.5	41.0
36-37	36.09251617327042	40.0	36.0	41.0	28.0	41.0
38-39	35.992343089035955	40.0	36.0	41.0	27.0	41.0
40-41	35.90836105596486	40.0	35.5	41.0	27.0	41.0
42-43	35.700903344276846	40.0	35.0	41.0	27.0	41.0
44-45	35.58231716484564	40.0	35.0	41.0	26.0	41.0
46-47	35.450453024573534	40.0	35.0	41.0	26.0	41.0
48-49	35.32559326248404	39.0	35.0	41.0	26.0	41.0
50-51	35.16790722267632	39.0	35.0	41.0	26.0	41.0
52-53	35.0828899245506	39.0	35.0	41.0	26.0	41.0
54-55	35.02078919326078	39.0	35.0	41.0	26.0	41.0
56-57	34.87334548099252	39.0	34.0	41.0	25.5	41.0
58-59	34.67661237383393	39.0	34.0	41.0	25.0	41.0
60-61	34.474640605814045	38.0	34.0	40.5	25.0	41.0
62-63	34.21359364803102	38.0	34.0	40.0	23.5	41.0
64-65	34.07396501984921	38.0	34.0	40.0	23.5	41.0
66-67	33.8190982786453	37.0	33.5	40.0	23.0	41.0
68-69	33.554593480658596	37.0	33.0	40.0	22.5	41.0
70-71	33.2556196425205	36.0	33.0	39.5	22.0	41.0
72-73	32.941773068527	36.0	33.0	39.0	21.5	41.0
74-75	32.594214406138875	35.0	33.0	39.0	20.0	40.5
76-77	32.255145191804814	35.0	32.0	38.0	20.0	40.0
78-79	31.9070651210553	35.0	32.0	37.0	18.0	39.0
80-81	31.592307335319745	35.0	32.0	37.0	17.5	39.0
82-83	31.272394643588036	35.0	32.0	36.0	14.5	39.0
84-85	30.969799643259194	35.0	32.0	36.0	9.5	37.0
86-87	30.6984867418108	35.0	31.0	36.0	7.0	37.0
88-89	30.421452833859593	35.0	31.0	35.0	2.0	37.0
90-91	30.193418474044023	35.0	31.0	35.0	2.0	36.0
92-93	29.99881438809725	35.0	31.0	35.0	2.0	36.0
94-95	29.771604395839773	35.0	31.0	35.0	2.0	36.0
96-97	29.669700743866777	35.0	31.0	35.0	2.0	36.0
98-99	29.51671788299555	35.0	31.0	35.0	2.0	35.0
100	29.384544261631525	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.27518289372329363
1101	2	0.3274557961792439
1101	3	0.32000377147135595
1101	4	0.3397677626966811
1101	5	0.2556300192097183
1101	6	0.2777539217264291
1101	7	0.37649090670806373
1101	8	0.40152207301852627
1101	9	0.5075667210802521
1101	10-11	0.5857234782841445
1101	12-13	0.48082819961185663
1101	14-15	0.6004388132275977
1101	16-17	0.6061158826546489
1101	18-19	0.5950721612722276
1101	20-21	0.5152794905087248
1101	22-23	0.515762114204982
1101	24-25	0.5354743502899595
1101	26-27	0.5976014470025532
1101	28-29	0.6201389987131236
1101	30-31	0.621121750515762
1101	32-33	0.6307522975439781
1101	34-35	0.586248630631033
1101	36-37	0.6009394066563374
1101	38-39	0.5862072527810227
1101	40-41	0.5605925132434919
1101	42-43	0.5768501443596037
1101	44-45	0.4680905658590291
1101	46-47	0.44311963632130613
1101	48-49	0.42430251644326944
1101	50-51	0.41517962144894227
1101	52-53	0.3471490030187496
1101	54-55	0.3733066374358671
1101	56-57	0.3856198810927509
1101	58-59	0.3626610433833548
1101	60-61	0.3876854573400479
1101	62-63	0.40962588976460523
1101	64-65	0.37183560802006355
1101	66-67	0.43938869192896135
1101	68-69	0.4169673466249506
1101	70-71	0.40297153197027313
1101	72-73	0.3839764590197987
1101	74-75	0.39073989108529616
1101	76-77	0.375506368982343
1101	78-79	0.32068275430201965
1101	80-81	0.3232897687001106
1101	82-83	0.3382498854205096
1101	84-85	0.30646244735185135
1101	86-87	0.31899923399008756
1101	88-89	0.3874402912878807
1101	90-91	0.34047914174034943
1101	92-93	0.3202369025147149
1101	94-95	0.33407523445727705
1101	96-97	0.36528247631279953
1101	98-99	0.34118511224998116
1101	100	0.34955226891512226
1102	1	0.12390840701516836
1102	2	0.22980337172085186
1102	3	0.18488417030162552
1102	4	0.17873969832540837
1102	5	0.17608691638493212
1102	6	0.23129231421758334
1102	7	0.23833021362106876
1102	8	0.25084107259200294
1102	9	0.3570954020450756
1102	10-11	0.35755723808794215
1102	12-13	0.33126672680180747
1102	14-15	0.4448152706307482
1102	16-17	0.34537280694238603
1102	18-19	0.3011331215201807
1102	20-21	0.3585965479407349
1102	22-23	0.3167246956997687
1102	24-25	0.3495771183904708
1102	26-27	0.3743472317835739
1102	28-29	0.4338863292630961
1102	30-31	0.40559243623631147
1102	32-33	0.4778260458218284
1102	34-35	0.4808246909518914
1102	36-37	0.3781354224096134
1102	38-39	0.3355285377315127
1102	40-41	0.4052253491933797
1102	42-43	0.3791459956963692
1102	44-45	0.27080140714745227
1102	46-47	0.2987774371486296
1102	48-49	0.2698149836721484
1102	50-51	0.261711046055666
1102	52-53	0.2203200700989143
1102	54-55	0.25161862306638483
1102	56-57	0.3165910822808655
1102	58-59	0.31357214929581545
1102	60-61	0.3032602471121706
1102	62-63	0.2785275527800337
1102	64-65	0.24601618482309107
1102	66-67	0.3072321103366278
1102	68-69	0.3012159061793156
1102	70-71	0.3170375146657207
1102	72-73	0.27280281691252384
1102	74-75	0.3029483565858655
1102	76-77	0.254511402018224
1102	78-79	0.24542385101818098
1102	80-81	0.26193915904751464
1102	82-83	0.24053444247086375
1102	84-85	0.22468687047793878
1102	86-87	0.22463137600045613
1102	88-89	0.2174996465459742
1102	90-91	0.23150288866003876
1102	92-93	0.22190649424946685
1102	94-95	0.21164792402252175
1102	96-97	0.25016995181690405
1102	98-99	0.31421834106631863
1102	100	0.33422939125964035
1103	1	0.1879481478497027
1103	2	0.20908799419147783
1103	3	0.2665866604324627
1103	4	0.3023348496801148
1103	5	0.22261664522432056
1103	6	0.26765350713664304
1103	7	0.30559593757864434
1103	8	0.3219583919620561
1103	9	0.3583351544695219
1103	10-11	0.44272933317770224
1103	12-13	0.32099043050367015
1103	14-15	0.45862295342810455
1103	16-17	0.4133975354751769
1103	18-19	0.31181884932339443
1103	20-21	0.34561459910033676
1103	22-23	0.30615406931358535
1103	24-25	0.3830418629736343
1103	26-27	0.39567646454345606
1103	28-29	0.39857419269142724
1103	30-31	0.33428653383089824
1103	32-33	0.42497327002152474
1103	34-35	0.3940108859272442
1103	36-37	0.40221844707503607
1103	38-39	0.46740213533067987
1103	40-41	0.3781913359743996
1103	42-43	0.4521008873465746
1103	44-45	0.4168812072067922
1103	46-47	0.3460387010212571
1103	48-49	0.3344879152557567
1103	50-51	0.28962092692636077
1103	52-53	0.32063437389161464
1103	54-55	0.38278360145072554
1103	56-57	0.3427390852480059
1103	58-59	0.41663014987802427
1103	60-61	0.4159350897971734
1103	62-63	0.3493155083056365
1103	64-65	0.4018687457199377
1103	66-67	0.4565511083392906
1103	68-69	0.40701956464366873
1103	70-71	0.41885723483005677
1103	72-73	0.40222534313164005
1103	74-75	0.3758600424690073
1103	76-77	0.3678958658321818
1103	78-79	0.3860050797453738
1103	80-81	0.41419343786909124
1103	82-83	0.39434641763938316
1103	84-85	0.39080323461069355
1103	86-87	0.33062567830937084
1103	88-89	0.3626998704467219
1103	90-91	0.4047681761629143
1103	92-93	0.3470279834627483
1103	94-95	0.3681824133311622
1103	96-97	0.37385401711690136
1103	98-99	0.3882368096964761
1103	100	0.4280503131891322
1104	1	0.09818180829068623
1104	2	0.14324944216880198
1104	3	0.09826169537377183
1104	4	0.09457680814385583
1104	5	0.11988005370519517
1104	6	0.13533273778150345
1104	7	0.18801989663162288
1104	8	0.1755286126841895
1104	9	0.2762789099959093
1104	10-11	0.3379712956697958
1104	12-13	0.23978968273313228
1104	14-15	0.30123364729024615
1104	16-17	0.3716848098810388
1104	18-19	0.26778562811841056
1104	20-21	0.22134919054658297
1104	22-23	0.22172664533530906
1104	24-25	0.2692367183753035
1104	26-27	0.36444164064343454
1104	28-29	0.40841470047519834
1104	30-31	0.3464580109203297
1104	32-33	0.3320563487604602
1104	34-35	0.34280667194965986
1104	36-37	0.345695088446746
1104	38-39	0.32478267981998243
1104	40-41	0.3131777659477706
1104	42-43	0.2287047039096919
1104	44-45	0.23904316550207483
1104	46-47	0.2110225162330579
1104	48-49	0.1839027204459498
1104	50-51	0.18678911084444394
1104	52-53	0.1800299693733436
1104	54-55	0.21490584210364005
1104	56-57	0.281809061431332
1104	58-59	0.29478480112322103
1104	60-61	0.3259792715773031
1104	62-63	0.3482870949294181
1104	64-65	0.3944605521411546
1104	66-67	0.3836861948324142
1104	68-69	0.3500466290739226
1104	70-71	0.30750466059911474
1104	72-73	0.3512434754411444
1104	74-75	0.3844465908024617
1104	76-77	0.3226031187376748
1104	78-79	0.35698476022810155
1104	80-81	0.36154692561862234
1104	82-83	0.36815241642978336
1104	84-85	0.3251261734122721
1104	86-87	0.3135950522735591
1104	88-89	0.34502400438476855
1104	90-91	0.30715939667978276
1104	92-93	0.2871230587936999
1104	94-95	0.26758394329627677
1104	96-97	0.3065352317212593
1104	98-99	0.3595767752101864
1104	100	0.35592697664350226
1105	1	0.14241215414494235
1105	2	0.1780663868080623
1105	3	0.17852383179466358
1105	4	0.17400428937864376
1105	5	0.12077486526055026
1105	6	0.23891721692294254
1105	7	0.2517046132889931
1105	8	0.2684281887732425
1105	9	0.3387022352793707
1105	10-11	0.39053660366666776
1105	12-13	0.2634346496324653
1105	14-15	0.3880522104921056
1105	16-17	0.35817903421214226
1105	18-19	0.3360584029146807
1105	20-21	0.28316834900420673
1105	22-23	0.27496907008387694
1105	24-25	0.30598637850203403
1105	26-27	0.37126959289432193
1105	28-29	0.44173148954836705
1105	30-31	0.40856045781240624
1105	32-33	0.4407260756340179
1105	34-35	0.4255735424307119
1105	36-37	0.38897213220408844
1105	38-39	0.35620757728940333
1105	40-41	0.322880661360621
1105	42-43	0.36712617092906186
1105	44-45	0.27417915390116576
1105	46-47	0.27234964249995386
1105	48-49	0.24815337873662457
1105	50-51	0.28539553715453536
1105	52-53	0.26577135064744795
1105	54-55	0.3073359435298926
1105	56-57	0.33572707925453926
1105	58-59	0.34077268402592864
1105	60-61	0.27307487658192287
1105	62-63	0.3688103007530401
1105	64-65	0.4168074972264151
1105	66-67	0.40552229497966863
1105	68-69	0.34705614410395214
1105	70-71	0.33377561160281033
1105	72-73	0.36023800761720537
1105	74-75	0.3919633516942582
1105	76-77	0.36617019419993824
1105	78-79	0.38595125585794676
1105	80-81	0.38619901639672705
1105	82-83	0.33935238362852616
1105	84-85	0.3476107780549462
1105	86-87	0.3450931217791755
1105	88-89	0.38531569836360546
1105	90-91	0.3782621683860512
1105	92-93	0.44024766732419707
1105	94-95	0.45811826174531234
1105	96-97	0.5222062457687642
1105	98-99	0.5199003802283215
1105	100	0.4731677067788844
1106	1	0.13820193855207918
1106	2	0.15333907374286326
1106	3	0.1580097430990044
1106	4	0.17250339122011837
1106	5	0.16786622625721037
1106	6	0.2427101667694629
1106	7	0.24660906945660344
1106	8	0.26559656886515626
1106	9	0.3946853566267663
1106	10-11	0.38977491465738723
1106	12-13	0.3393411895326679
1106	14-15	0.4542332365325805
1106	16-17	0.36381060450464275
1106	18-19	0.37019413916365806
1106	20-21	0.36852599803275865
1106	22-23	0.32742337779114195
1106	24-25	0.3423165961095762
1106	26-27	0.3879887029683289
1106	28-29	0.4810544900376712
1106	30-31	0.4541410432665387
1106	32-33	0.4276093351934378
1106	34-35	0.3458184375962077
1106	36-37	0.3981546306453083
1106	38-39	0.3311077948643586
1106	40-41	0.3631421207873444
1106	42-43	0.33531736238803234
1106	44-45	0.3069731863657168
1106	46-47	0.30487433357446037
1106	48-49	0.2684054545625827
1106	50-51	0.3174296471915312
1106	52-53	0.3240308823423632
1106	54-55	0.35373901247523065
1106	56-57	0.3077732314473849
1106	58-59	0.37548934198132855
1106	60-61	0.36493867851151407
1106	62-63	0.42436893099181106
1106	64-65	0.4206647532270722
1106	66-67	0.4378971446773008
1106	68-69	0.47316233052102774
1106	70-71	0.41580222247885246
1106	72-73	0.3942217533631265
1106	74-75	0.4213931849890358
1106	76-77	0.39020229502744996
1106	78-79	0.36614688600181466
1106	80-81	0.3405075188377076
1106	82-83	0.30198682447450054
1106	84-85	0.35313367993030553
1106	86-87	0.3483922411093836
1106	88-89	0.35392192047767423
1106	90-91	0.3167957707521616
1106	92-93	0.2729121281105371
1106	94-95	0.3218886636474707
1106	96-97	0.41662719754162225
1106	98-99	0.4030337042722678
1106	100	0.373270208923131
1107	1	0.06277953948308479
1107	2	0.07568604356746178
1107	3	0.14559684803395356
1107	4	0.14374006871783962
1107	5	0.08031442828072244
1107	6	0.16364779552386466
1107	7	0.24616601492079582
1107	8	0.2186180519446026
1107	9	0.327837789092996
1107	10-11	0.3137269646016989
1107	12-13	0.29629897282520545
1107	14-15	0.3281362913511572
1107	16-17	0.2729449955731198
1107	18-19	0.3432312500030221
1107	20-21	0.3236418030642696
1107	22-23	0.39233554700248163
1107	24-25	0.36083140432189253
1107	26-27	0.3737997688222805
1107	28-29	0.41086477188994763
1107	30-31	0.3865407758855284
1107	32-33	0.42649682000178757
1107	34-35	0.38642309514759177
1107	36-37	0.32471350604400584
1107	38-39	0.3909807943607433
1107	40-41	0.3701123202607164
1107	42-43	0.3977595629255788
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2216	100	-1.5151655285840562
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	184244.0
3	37018.0
4	24451.0
5	20684.0
6	15929.0
7	16107.0
8	18104.0
9	19085.0
10	19171.0
11	19875.0
12	21791.0
13	23790.0
14	25657.0
15	26491.0
16	26993.0
17	26361.0
18	26121.0
19	24446.0
20	23399.0
21	25248.0
22	29891.0
23	43232.0
24	63972.0
25	80478.0
26	88301.0
27	77476.0
28	83089.0
29	100011.0
30	117348.0
31	136535.0
32	171664.0
33	223158.0
34	306032.0
35	459279.0
36	742554.0
37	1307007.0
38	1815065.0
39	810764.0
40	2338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.95560268506365	21.57707528444553	14.218129425429227	22.249192605061587
2	15.363913676019369	22.05843682823291	39.764162764081064	22.813486731666657
3	17.91542909558786	27.563361579477125	30.38469407792201	24.13651524701301
4	12.12152378608861	19.13139073822532	38.78164232792841	29.965443147757664
5	12.322113938808046	39.76290555357969	34.73676694134981	13.178213566262444
6	28.481237062777847	39.79068649085548	18.447994735716083	13.28008171065059
7	25.26907655669341	34.81369552676746	22.586295987027366	17.330931929511767
8	23.981125966310373	37.12636616767807	20.864298702193913	18.02820916381764
9	24.549160344482647	18.689717493085315	21.719299011670003	35.04182315076203
10-11	22.57000377262243	29.066940190374975	28.874210332454197	19.488845704548396
12-13	23.166133519143706	27.171993438965952	28.543745524921516	21.118127516968823
14-15	21.082841918163144	29.08890594165688	26.032919318155056	23.79533282202492
16-17	20.278342044723995	32.06547425015811	26.760572373105845	20.895611332012045
18-19	20.231891974241304	30.872832350496438	28.671265345090713	20.224010330171545
20-21	21.950820643430475	29.9146968576541	27.92281640906512	20.2116660898503
22-23	21.46551499231744	30.00037760565063	27.96822757400041	20.565879828031523
24-25	21.143543618425355	29.98474614252752	28.083904592057834	20.787805646989295
26-27	20.920437946382105	29.892630745828686	28.170045853759547	21.016885454029662
28-29	20.85541328093356	29.945713145715946	28.316036377815852	20.88283719553464
30-31	21.21966080865606	30.34339647901466	27.836071820274245	20.60087089205504
32-33	20.749082991539844	30.101693933137796	28.294322041246723	20.854901034075635
34-35	21.06940504323436	29.67145289388095	28.275377081623688	20.983764981261004
36-37	21.196961117039944	30.008152223166316	27.878680858519587	20.916205801274145
38-39	21.05043967133657	29.995594395785837	28.064529572163448	20.889436360714146
40-41	20.97448431401492	29.684213778401773	28.30739656243671	21.033905345146593
42-43	20.869010426070957	29.781199568682432	28.295830134586954	21.053959870659654
44-45	21.150359837585984	29.796643328336675	28.090351480989156	20.962645353088185
46-47	21.027349318884525	29.9666848489696	28.04318714601579	20.962778686130086
48-49	20.930563853142708	29.725341817491284	28.107107505286006	21.236986824080002
50-51	20.937799675073954	29.663280555735582	28.099113977861528	21.299805791328936
52-53	20.93454033872091	29.68827921956664	28.078082057106702	21.299098384605752
54-55	21.09311038500384	29.943922161507828	27.894710066775612	21.068257386712723
56-57	20.929695624172254	29.71048764762812	27.918810685802125	21.441006042397497
58-59	20.973056692815543	29.453908102832944	28.132058625747504	21.440976578604012
60-61	21.132186250563734	29.81773973712017	27.794908065245078	21.255165947071017
62-63	20.97193534527916	29.74620939118896	28.010747867651673	21.271107395880204
64-65	21.06023923673606	29.45037971513264	28.106258997191752	21.38312205093955
66-67	20.903480976267492	29.579273761944435	27.87473606993327	21.642509191854803
68-69	21.31850399541776	29.74214068821846	27.81216232999316	21.12719298637062
70-71	21.251354990246544	29.560465158908972	27.884811909263213	21.30336794158127
72-73	21.09704971582571	29.630091134180997	28.078747017431482	21.194112132561806
74-75	21.14502011369671	29.647455074147267	27.88710928127217	21.320415530883853
76-77	21.15673011781719	29.589935600519457	27.65627604532102	21.59705823634233
78-79	21.2744070050431	29.618383384312168	27.896135680110373	21.21107393053436
80-81	21.032081592407735	29.56817859299823	27.999260183140372	21.400479631453667
82-83	21.030493963948942	29.585798166601368	28.114371055514454	21.26933681393524
84-85	21.096830112642646	29.33291454914621	28.011973108562938	21.558282229648203
86-87	21.381883414721553	29.474350868015147	27.78983783457218	21.353927882691117
88-89	21.176996629290777	29.36925900554947	27.890178231965596	21.56356613319416
90-91	21.128759140724682	29.220825628458613	27.852693894237916	21.797721336578793
92-93	21.23864126530886	29.39400012069208	27.768549662216646	21.598808951782416
94-95	21.162346290742416	29.203596993146054	27.929442803833982	21.70461391227755
96-97	21.2050358551558	29.28023453900445	27.801129553773674	21.713600052066077
98-99	21.08375297352405	29.350661298018338	27.826081882184944	21.73950384627267
100	21.209377991140148	29.414303264040605	27.607429774156607	21.76888897066264
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	30126.0
1	22373.5
2	13327.5
3	10328.5
4	7241.0
5	5246.0
6	4516.0
7	4669.5
8	5251.0
9	5392.5
10	4381.5
11	3303.0
12	3065.0
13	3107.5
14	3217.0
15	3434.0
16	3869.5
17	4544.0
18	5565.5
19	7142.0
20	9242.0
21	12266.0
22	17029.0
23	24259.0
24	33621.5
25	45805.5
26	62623.0
27	83188.0
28	108035.5
29	133685.0
30	153867.0
31	171877.0
32	188903.5
33	204284.0
34	218062.5
35	230106.5
36	239938.0
37	247327.5
38	251927.0
39	253246.5
40	253627.5
41	251151.5
42	244405.0
43	236708.5
44	229619.0
45	225542.0
46	223506.5
47	217421.5
48	213405.0
49	214127.5
50	213268.0
51	205661.0
52	195696.0
53	186049.0
54	177958.5
55	172118.0
56	156376.0
57	136730.0
58	122313.0
59	109533.0
60	94103.5
61	77838.5
62	64870.0
63	52972.5
64	42019.5
65	32736.0
66	25466.5
67	19788.5
68	15185.0
69	11575.0
70	8673.5
71	6609.5
72	5194.5
73	4066.5
74	3143.5
75	2338.5
76	1662.0
77	1223.0
78	989.0
79	765.5
80	510.0
81	338.5
82	249.5
83	209.5
84	171.5
85	138.5
86	120.5
87	94.5
88	73.0
89	60.5
90	54.5
91	46.5
92	37.0
93	26.0
94	22.5
95	25.0
96	29.0
97	27.5
98	28.5
99	35.5
100	56.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015062145423435078
2	0.008155801623993104
3	0.010283999017459319
4	0.00915811394478687
5	0.011382423478603172
6	0.012041478155289484
7	0.010325189934752215
8	0.007702701533771266
9	0.006453243709220134
10-11	0.005855975408473164
12-13	0.0063914573332807915
14-15	0.007352578736781663
16-17	0.004901719157854442
18-19	0.005691211739301586
20-21	0.005045887368379572
22-23	0.005890301172883909
24-25	0.005643155669126543
26-27	0.006055064842055488
28-29	0.01638711992968985
30-31	0.005444066235544219
32-33	0.0051419995087296595
34-35	0.0048399327819151
36-37	0.006631737684156009
38-39	0.005347954095194132
40-41	0.005272437413490492
42-43	0.00491544946361874
44-45	0.003981788671646465
46-47	0.005993278466116145
48-49	0.0034600370526031354
50-51	0.0037209128621248006
52-53	0.003171700631552874
54-55	0.0046065175839220315
56-57	0.0028215778345632714
58-59	0.0024920504962201153
60-61	0.00342571128819239
62-63	0.0031030491027313834
64-65	0.00454473120798269
66-67	0.002972611197970551
68-69	0.003480632511249583
70-71	0.0049085843107365915
72-73	0.002972611197970551
74-75	0.0025607020250416066
76-77	0.0024851853433379666
78-79	0.0032197567017279174
80-81	0.0037826992380641422
82-83	0.001750613984948015
84-85	0.002354747438577134
86-87	0.0022517701453448977
88-89	0.0016750973032443753
90-91	0.002217444380934152
92-93	0.0022311746866984505
94-95	0.0018055352080052077
96-97	0.0014554124110156047
98-99	0.0018398609724159531
100	0.002636218706745246
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7283159.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.569400122139115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.44873513813408	41.13260821387981
2	16.39871527867874	18.88128402734585
3	5.885880762044235	10.16539873983977
4	2.4802452098805197	5.711449155545221
5	1.2729359057181104	3.664107824306172
6	0.7167637645379913	2.4758195972242993
7	0.4396926203838738	1.7718988275541736
8	0.28247804627165696	1.3009673337226517
9	0.18790199942422225	0.9735664849742705
>10	0.8249365838883218	7.975132483035807
>50	0.037962510109285794	1.5067620261508041
>100	0.02088765564349576	2.3758733489699733
>500	0.001933651772906283	0.746121376670202
>1k	9.070049622526669E-4	0.815431080353283
>5k	0.0	0.0
>10k+	2.3868550322434008E-5	0.5035794804276743
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36648	0.5031882456499989	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.3730305764298157E-5	0.0
5	0.0	0.0	0.0	2.7460611528596314E-5	0.0
6	0.0	0.0	0.0	2.7460611528596314E-5	0.0
7	0.0	0.0	0.0	2.7460611528596314E-5	0.0
8	0.0	0.0	0.0	2.7460611528596314E-5	0.0
9	0.0	0.0	0.0	2.7460611528596314E-5	0.0
10-11	0.0	0.0	0.0	5.492122305719263E-5	0.0
12-13	0.0	0.0	0.0	6.865152882149078E-5	0.0
14-15	0.0	0.0	0.0	8.238183458578894E-5	6.8651528821490785E-6
16-17	0.0	0.0	0.0	9.61121403500871E-5	1.3730305764298157E-5
18-19	0.0	0.0	0.0	1.304379047608325E-4	1.3730305764298157E-5
20-21	0.0	0.0	0.0	2.3341519799306866E-4	1.3730305764298157E-5
22-23	0.0	6.8651528821490785E-6	0.0	4.531000902218392E-4	1.3730305764298157E-5
24-25	0.0	1.3730305764298157E-5	0.0	0.0012700532831975795	1.3730305764298157E-5
26-27	0.0	1.3730305764298157E-5	0.0	0.00238220805010573	1.3730305764298157E-5
28-29	0.0	1.3730305764298157E-5	0.0	0.005450931388426368	1.3730305764298157E-5
30-31	0.0	1.3730305764298157E-5	0.0	0.013888204280587586	1.3730305764298157E-5
32-33	0.0	1.3730305764298157E-5	0.0	0.03164835478670725	1.3730305764298157E-5
34-35	0.0	1.3730305764298157E-5	0.0	0.059362976971943086	1.3730305764298157E-5
36-37	0.0	1.3730305764298157E-5	0.0	0.10375305550791902	1.3730305764298157E-5
38-39	0.0	2.0595458646447236E-5	0.0	0.16104275630945308	1.3730305764298157E-5
40-41	0.0	2.7460611528596314E-5	0.0	0.22108538341672893	1.3730305764298157E-5
42-43	6.8651528821490785E-6	2.7460611528596314E-5	0.0	0.28404570049891814	1.3730305764298157E-5
44-45	1.3730305764298157E-5	2.7460611528596314E-5	0.0	0.363523575415558	1.3730305764298157E-5
46-47	1.3730305764298157E-5	2.7460611528596314E-5	0.0	0.46013000677315985	2.7460611528596314E-5
48-49	2.7460611528596314E-5	2.7460611528596314E-5	0.0	0.5638967925868431	2.7460611528596314E-5
50-51	4.119091729289447E-5	2.7460611528596314E-5	0.0	0.667498814731355	2.7460611528596314E-5
52-53	4.119091729289447E-5	2.7460611528596314E-5	0.0	0.7740391223094265	2.7460611528596314E-5
54-55	4.119091729289447E-5	2.7460611528596314E-5	0.0	0.8970352013460093	2.7460611528596314E-5
56-57	4.119091729289447E-5	2.7460611528596314E-5	0.0	1.0480342389888784	2.7460611528596314E-5
58-59	4.119091729289447E-5	2.7460611528596314E-5	0.0	1.2116857533935481	2.7460611528596314E-5
60-61	4.119091729289447E-5	2.7460611528596314E-5	0.0	1.3838843831364933	2.7460611528596314E-5
62-63	4.805607017504355E-5	2.7460611528596314E-5	0.0	1.558485816388191	2.7460611528596314E-5
64-65	5.492122305719263E-5	2.7460611528596314E-5	0.0	1.7478816541009197	2.7460611528596314E-5
66-67	5.492122305719263E-5	2.7460611528596314E-5	0.0	1.9719259184098548	2.7460611528596314E-5
68-69	5.492122305719263E-5	2.7460611528596314E-5	0.0	2.2270075388989863	2.7460611528596314E-5
70-71	5.492122305719263E-5	4.119091729289447E-5	0.0	2.4997943886711798	2.7460611528596314E-5
72-73	5.492122305719263E-5	4.119091729289447E-5	0.0	2.7861467805385	2.7460611528596314E-5
74-75	5.492122305719263E-5	4.119091729289447E-5	0.0	3.0795496844157872	2.7460611528596314E-5
76-77	5.492122305719263E-5	4.119091729289447E-5	0.0	3.3984634964031404	2.7460611528596314E-5
78-79	5.492122305719263E-5	4.119091729289447E-5	0.0	3.7599687168713465	2.7460611528596314E-5
80-81	5.492122305719263E-5	4.119091729289447E-5	0.0	4.160392489028456	2.7460611528596314E-5
82-83	5.492122305719263E-5	4.119091729289447E-5	0.0	4.575404711060132	2.7460611528596314E-5
84-85	5.492122305719263E-5	4.119091729289447E-5	0.0	4.993547442806069	2.7460611528596314E-5
86-87	5.492122305719263E-5	4.119091729289447E-5	0.0	5.436947072005431	2.7460611528596314E-5
88	5.492122305719263E-5	4.119091729289447E-5	0.0	5.793488786939843	2.7460611528596314E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9900	0.0	46.011215	1
GTATCAA	16905	0.0	34.53676	1
TCAACGC	18325	0.0	30.88423	4
ATCAACG	18635	0.0	30.47115	3
TATCAAC	20020	0.0	28.644882	2
CAACGCA	20815	0.0	27.189695	5
GTGGTAT	5005	0.0	26.11054	1
AACGCAG	22070	0.0	25.598684	6
GTACATG	31490	0.0	25.16866	1
CATGGGT	15440	0.0	25.086159	4
TACATGG	31470	0.0	24.302004	2
CGCAGAG	23270	0.0	23.631432	8
ACGCAGA	23765	0.0	23.495363	7
ACATGGG	32315	0.0	22.953773	3
TGGTATC	5460	0.0	22.469795	2
GCAGAGT	25600	0.0	20.379032	9
ATGGGTG	9140	0.0	20.00588	5
ATGGGTA	6850	0.0	19.557283	5
CAGAGTA	24955	0.0	18.391167	10-11
TACCTGG	5090	0.0	17.269316	2
>>END_MODULE