FastQCFastQC Report
Wed 25 May 2016
SRR2431443_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431443_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7283159
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT486980.6686384301097916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT422810.5805310580202904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292950.4022293073651145No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107300.14732618085091922No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106600.14636505944741834No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101010.13868981852517567No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94120.12922963785357425No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76760.10539382704675265No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA187350.061.2370031
GTATCAA352350.042.5505751
GTGGTAT64900.038.066991
TGGTATC66600.036.1556322
ATCAACG462350.032.0623663
TATCAAC463500.032.0436972
TCAACGC465600.031.8080444
CAACGCA488950.030.264245
AACGCAG508450.029.1578186
CATGGGT146850.026.1012274
ACGCAGA570850.025.8635147
CGCAGAG571400.025.748138
GTACATG302400.025.4430921
TACATGG303500.024.5767672
GCAGAGT606650.023.9343349
ACATGGG309400.023.5608883
ATGGGTG88900.021.4676115
GAGTACT442200.021.46264612-13
CAGAGTA601150.021.13512410-11
AGAGTAC584600.021.04599810-11
Produced by FastQC (version 0.11.5)