##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431443_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7283159
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.260844504424526	33.0	31.0	34.0	30.0	34.0
2	32.40564554474233	34.0	31.0	34.0	31.0	34.0
3	32.51070805951099	34.0	31.0	34.0	31.0	34.0
4	36.05161249397411	37.0	35.0	37.0	35.0	37.0
5	35.96158081953175	37.0	35.0	37.0	35.0	37.0
6	35.998399458257055	37.0	35.0	37.0	35.0	37.0
7	35.93692022925766	37.0	35.0	37.0	35.0	37.0
8	35.92153912883132	37.0	35.0	37.0	35.0	37.0
9	37.61315975663857	39.0	37.0	39.0	35.0	39.0
10-11	37.551253377826846	39.0	37.0	39.0	34.5	39.0
12-13	37.53210131757387	39.0	37.0	39.0	35.0	39.0
14-15	38.87417527202139	40.0	38.0	41.0	35.0	41.0
16-17	38.927616917329416	40.0	38.0	41.0	35.0	41.0
18-19	39.01109710772482	40.5	38.5	41.0	35.0	41.0
20-21	39.03575048958838	41.0	39.0	41.0	35.0	41.0
22-23	38.976374400174436	40.5	39.0	41.0	35.0	41.0
24-25	38.91848145838914	41.0	38.0	41.0	35.0	41.0
26-27	38.7771460845493	40.0	38.0	41.0	34.5	41.0
28-29	38.682004196256045	40.0	38.0	41.0	34.0	41.0
30-31	38.50237273413913	40.0	38.0	41.0	34.0	41.0
32-33	38.29330644847929	40.0	38.0	41.0	34.0	41.0
34-35	38.14210571264475	40.0	38.0	41.0	34.0	41.0
36-37	37.99320418516196	40.0	37.0	41.0	33.0	41.0
38-39	37.88137448324278	40.0	37.0	41.0	33.0	41.0
40-41	37.74781128628388	40.0	37.0	41.0	33.0	41.0
42-43	37.61297316178323	40.0	37.0	41.0	33.0	41.0
44-45	37.38860266540934	40.0	37.0	41.0	32.5	41.0
46-47	37.061650239957686	40.0	37.0	41.0	31.0	41.0
48-49	36.80280081761225	40.0	37.0	41.0	31.0	41.0
50-51	36.667522842766445	40.0	36.0	41.0	30.5	41.0
52-53	36.53355721054559	40.0	36.0	41.0	30.0	41.0
54-55	36.38512361188325	40.0	36.0	41.0	30.0	41.0
56-57	36.169167870700065	39.5	35.0	41.0	30.0	41.0
58-59	36.00727404138781	39.0	35.0	41.0	29.0	41.0
60-61	35.81119312375303	39.0	35.0	41.0	29.0	41.0
62-63	35.595967697533446	39.0	35.0	41.0	28.5	41.0
64-65	35.3616183581877	38.5	35.0	41.0	28.0	41.0
66-67	35.108857365327324	38.0	35.0	40.5	28.0	41.0
68-69	34.82501892379392	37.0	35.0	40.0	28.0	41.0
70-71	34.50300693696238	37.0	34.5	40.0	28.0	41.0
72-73	34.18394593609723	36.0	34.0	39.0	27.0	41.0
74-75	33.87159527891674	36.0	34.0	39.0	27.0	41.0
76-77	33.512774264573935	35.0	34.0	38.5	26.0	40.0
78-79	33.194030021862766	35.0	34.0	37.0	26.0	39.5
80-81	32.883721679013185	35.0	34.0	37.0	26.0	39.0
82-83	32.616894056548816	35.0	34.0	36.5	26.0	39.0
84-85	32.38109665050564	35.0	34.0	36.0	26.0	37.5
86-87	32.130043433076224	35.0	33.0	36.0	25.5	37.0
88-89	31.97113738694981	35.0	33.0	35.5	26.0	37.0
90-91	31.779939652560103	35.0	33.0	35.0	25.0	36.0
92-93	31.58302262795581	35.0	33.0	35.0	25.0	36.0
94-95	31.424049440634207	35.0	33.0	35.0	24.5	36.0
96-97	31.257289659610617	35.0	33.0	35.0	23.5	36.0
98-99	31.092409214188514	35.0	33.0	35.0	21.5	35.5
100	30.98481634137055	35.0	33.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15844100458959787
1101	2	0.22539690249698197
1101	3	0.21022897434374244
1101	4	0.18234135282567365
1101	5	0.18895723748680382
1101	6	0.21440993065937874
1101	7	0.23255745063145383
1101	8	0.2750308379450246
1101	9	0.3691259035713088
1101	10-11	0.32545017987958147
1101	12-13	0.3456864445687913
1101	14-15	0.4292532057883989
1101	16-17	0.3736636336427708
1101	18-19	0.3978237506035782
1101	20-21	0.3351394757259172
1101	22-23	0.3989896644308928
1101	24-25	0.34712269305628496
1101	26-27	0.36257490433626316
1101	28-29	0.3563053930101958
1101	30-31	0.3394444228571416
1101	32-33	0.345082611952094
1101	34-35	0.40108492788215244
1101	36-37	0.4648618178519399
1101	38-39	0.38951059460929827
1101	40-41	0.4172085461074033
1101	42-43	0.42650047309855665
1101	44-45	0.44043202126798064
1101	46-47	0.4138048807612762
1101	48-49	0.34713687549339767
1101	50-51	0.33173566883124295
1101	52-53	0.33469010361160656
1101	54-55	0.30761462832310116
1101	56-57	0.3523375856249302
1101	58-59	0.39028714526353525
1101	60-61	0.4282322452223113
1101	62-63	0.4408119862026396
1101	64-65	0.41238898657220346
1101	66-67	0.43707203369294945
1101	68-69	0.3575666859613733
1101	70-71	0.3694143416076656
1101	72-73	0.39652738153211686
1101	74-75	0.35437477345813306
1101	76-77	0.3763815975110987
1101	78-79	0.363035759309561
1101	80-81	0.3667786175458545
1101	82-83	0.32224908410968567
1101	84-85	0.35502836835348006
1101	86-87	0.33518988937884586
1101	88-89	0.3373191834277378
1101	90-91	0.3818373262461101
1101	92-93	0.4140504943445329
1101	94-95	0.3916844528550989
1101	96-97	0.44497056122465395
1101	98-99	0.4735507068856073
1101	100	0.5016307166107339
1102	1	0.17168619763931048
1102	2	0.2408659311826966
1102	3	0.21505733053738396
1102	4	0.15526108790835735
1102	5	0.1870530913858488
1102	6	0.14897658368983002
1102	7	0.1925120585979414
1102	8	0.2427689681365166
1102	9	0.2886585977907714
1102	10-11	0.2638642173438157
1102	12-13	0.2908826600780543
1102	14-15	0.42461893601793577
1102	16-17	0.39705895236774325
1102	18-19	0.37139246840976625
1102	20-21	0.3521578954551785
1102	22-23	0.3580539747256495
1102	24-25	0.32757250166277885
1102	26-27	0.3354124818701578
1102	28-29	0.3437213554365641
1102	30-31	0.35453243024959136
1102	32-33	0.3753628385639942
1102	34-35	0.3745397083341544
1102	36-37	0.3188565389040221
1102	38-39	0.2988831743789504
1102	40-41	0.38174328263882273
1102	42-43	0.3905401388586611
1102	44-45	0.35727142207255014
1102	46-47	0.3924209619336594
1102	48-49	0.2951737444356226
1102	50-51	0.17467452756660862
1102	52-53	0.19786309473742847
1102	54-55	0.19411369437024462
1102	56-57	0.24903438124361088
1102	58-59	0.25957288129153966
1102	60-61	0.2739326223114702
1102	62-63	0.305254185439658
1102	64-65	0.319159873255785
1102	66-67	0.31282330020033555
1102	68-69	0.2802421684503855
1102	70-71	0.25268402075613494
1102	72-73	0.27018251661925063
1102	74-75	0.27219380541104243
1102	76-77	0.28258502040137046
1102	78-79	0.24660224009824816
1102	80-81	0.22325930034786978
1102	82-83	0.20591892848389648
1102	84-85	0.22376233305535465
1102	86-87	0.18325671391982468
1102	88-89	0.21887184634337586
1102	90-91	0.2207176761708638
1102	92-93	0.25803327995664915
1102	94-95	0.26053323727729705
1102	96-97	0.2830286190437974
1102	98-99	0.3431802780808262
1102	100	0.3253421934562688
1103	1	0.1385542452513775
1103	2	0.16818220688489305
1103	3	0.14205501653355412
1103	4	0.14037555916412003
1103	5	0.12087400267890303
1103	6	0.11794222167177537
1103	7	0.1314494178141672
1103	8	0.14211010084443387
1103	9	0.23716531027843502
1103	10-11	0.22128227242369292
1103	12-13	0.2074216111810685
1103	14-15	0.29486379329468804
1103	16-17	0.28826960873044527
1103	18-19	0.24997086771240618
1103	20-21	0.28436339349848794
1103	22-23	0.22300926186128578
1103	24-25	0.23973257510279922
1103	26-27	0.2672698598562775
1103	28-29	0.27297784976098427
1103	30-31	0.24129484420095082
1103	32-33	0.26827550964689095
1103	34-35	0.2668598540365892
1103	36-37	0.2948128880401697
1103	38-39	0.26399193196998283
1103	40-41	0.31317161819359995
1103	42-43	0.305287510764245
1103	44-45	0.3267279647490611
1103	46-47	0.3410354300507237
1103	48-49	0.2799125026336
1103	50-51	0.25959449642122934
1103	52-53	0.2774600978953856
1103	54-55	0.28500170968496263
1103	56-57	0.3351912249872555
1103	58-59	0.370779634842485
1103	60-61	0.32670780912025776
1103	62-63	0.3550118205074497
1103	64-65	0.31386687001545965
1103	66-67	0.3046279890882815
1103	68-69	0.25677857839521323
1103	70-71	0.2994286483096502
1103	72-73	0.27231241990756416
1103	74-75	0.27950381378409617
1103	76-77	0.29434313348192376
1103	78-79	0.2900957417068142
1103	80-81	0.2812387284007656
1103	82-83	0.2279169793002822
1103	84-85	0.262640790166067
1103	86-87	0.28031784045519004
1103	88-89	0.23701230450761557
1103	90-91	0.23880868441112924
1103	92-93	0.2951505096957412
1103	94-95	0.29785782525814497
1103	96-97	0.32288456924051445
1103	98-99	0.3689137070167128
1103	100	0.3537423379864215
1104	1	0.07113981414786963
1104	2	0.1495871047288304
1104	3	0.1408956224624376
1104	4	0.10401216333590924
1104	5	0.03868362350002741
1104	6	0.08177485971900467
1104	7	0.11688163442335764
1104	8	0.08423878043927857
1104	9	0.14573637237518255
1104	10-11	0.1724231406893395
1104	12-13	0.15916088539429296
1104	14-15	0.28587458907394137
1104	16-17	0.24029968361553244
1104	18-19	0.23746599021160364
1104	20-21	0.23043554279038148
1104	22-23	0.1925329477009754
1104	24-25	0.23279890215564336
1104	26-27	0.21661873295446554
1104	28-29	0.2380190963430735
1104	30-31	0.2187109305876973
1104	32-33	0.16998364659081489
1104	34-35	0.23567131489546256
1104	36-37	0.23849446226579119
1104	38-39	0.28140729691691035
1104	40-41	0.2225411896565177
1104	42-43	0.2232827970522564
1104	44-45	0.1954375405442832
1104	46-47	0.2201244145253014
1104	48-49	0.17456780088578938
1104	50-51	0.15036459863680562
1104	52-53	0.16202313516434685
1104	54-55	0.18230633087321024
1104	56-57	0.2516825381377288
1104	58-59	0.25020361733310637
1104	60-61	0.20202156677617467
1104	62-63	0.1895790783824438
1104	64-65	0.20459457848639317
1104	66-67	0.14359564532989566
1104	68-69	0.16140888992721614
1104	70-71	0.17287143694188245
1104	72-73	0.13863220341703197
1104	74-75	0.1411278617902454
1104	76-77	0.1872592902731327
1104	78-79	0.21741549115845515
1104	80-81	0.20355932105808705
1104	82-83	0.20426259731327434
1104	84-85	0.21706466737041552
1104	86-87	0.21248508869878435
1104	88-89	0.1737279561444609
1104	90-91	0.19110621388258764
1104	92-93	0.20521670088352195
1104	94-95	0.21486853387867555
1104	96-97	0.21559283142931918
1104	98-99	0.26543168414340457
1104	100	0.3062366429148611
1105	1	0.0783673740274935
1105	2	0.14375357164424685
1105	3	0.10841196489832328
1105	4	0.08644089745079953
1105	5	0.07421314258845513
1105	6	0.0746197167003686
1105	7	0.0745919378385409
1105	8	0.0988341140250455
1105	9	0.11715900520739098
1105	10-11	0.12703125095131895
1105	12-13	0.07621653541091433
1105	14-15	0.1799321686076567
1105	16-17	0.17623836552026972
1105	18-19	0.1781459464221058
1105	20-21	0.14554521733118975
1105	22-23	0.14696020682540478
1105	24-25	0.15864991354848001
1105	26-27	0.18047394152230112
1105	28-29	0.16141271427736825
1105	30-31	0.16492266963538782
1105	32-33	0.19197876442746775
1105	34-35	0.21209119089390782
1105	36-37	0.2160160722709179
1105	38-39	0.18653425253197042
1105	40-41	0.2009952678070306
1105	42-43	0.2556051891701401
1105	44-45	0.25577894104426235
1105	46-47	0.2496635550790458
1105	48-49	0.25020306316169894
1105	50-51	0.19984342112496023
1105	52-53	0.18126784267433038
1105	54-55	0.2475112463353426
1105	56-57	0.2953197560509082
1105	58-59	0.25718623835259535
1105	60-61	0.2615352907974824
1105	62-63	0.22670207048260238
1105	64-65	0.22593349792482798
1105	66-67	0.271468096413642
1105	68-69	0.2479272665385963
1105	70-71	0.23160848990703187
1105	72-73	0.25094349623612544
1105	74-75	0.23317180214881006
1105	76-77	0.2389187478298922
1105	78-79	0.20803371401617454
1105	80-81	0.17662179910247033
1105	82-83	0.17257750644437664
1105	84-85	0.13343416962820953
1105	86-87	0.15280109931957497
1105	88-89	0.15173332250839167
1105	90-91	0.1432032554970064
1105	92-93	0.14570379422832858
1105	94-95	0.16661923437031945
1105	96-97	0.18617576717030815
1105	98-99	0.1891280118418308
1105	100	0.2284336412977197
1106	1	0.10621787465397148
1106	2	0.16181157874387964
1106	3	0.09936725553424708
1106	4	0.09515281892117855
1106	5	0.07765602556663254
1106	6	0.10522887010222348
1106	7	0.09155620303634748
1106	8	0.140382577676192
1106	9	0.1387424217312514
1106	10-11	0.12841182813044583
1106	12-13	0.156265000269876
1106	14-15	0.21825029786173644
1106	16-17	0.20568659103705755
1106	18-19	0.19308794404195595
1106	20-21	0.15011569094883015
1106	22-23	0.1479204622007373
1106	24-25	0.19019945150483153
1106	26-27	0.17459316285399495
1106	28-29	0.12467881340106857
1106	30-31	0.17571690956798136
1106	32-33	0.1462793666805382
1106	34-35	0.17338282330458554
1106	36-37	0.1618853042859172
1106	38-39	0.15716528678940733
1106	40-41	0.1778871537011142
1106	42-43	0.2539653628975955
1106	44-45	0.2213684519054553
1106	46-47	0.202570612325502
1106	48-49	0.19330550580953343
1106	50-51	0.17982624479258646
1106	52-53	0.17888336136675065
1106	54-55	0.18966114467264816
1106	56-57	0.2203441187604156
1106	58-59	0.18788902660400453
1106	60-61	0.18469581521419087
1106	62-63	0.1648866719469666
1106	64-65	0.14710768815363906
1106	66-67	0.16234183802610858
1106	68-69	0.19889729055216065
1106	70-71	0.21341612373566932
1106	72-73	0.23604934246372267
1106	74-75	0.22154934915456437
1106	76-77	0.2088591861727167
1106	78-79	0.16394417512606907
1106	80-81	0.16909720108513682
1106	82-83	0.152767329649258
1106	84-85	0.17970003405874024
1106	86-87	0.1780540542670792
1106	88-89	0.18621410526691307
1106	90-91	0.22890908016284683
1106	92-93	0.2553241023430495
1106	94-95	0.2602740664953238
1106	96-97	0.22530098357549377
1106	98-99	0.2634672111979306
1106	100	0.3401756957439055
1107	1	0.0922452404342593
1107	2	0.11549815391514073
1107	3	0.052101633101244715
1107	4	0.08852397729807393
1107	5	0.0712950229684921
1107	6	0.09580394836797268
1107	7	0.028173740657777557
1107	8	0.012560012367778484
1107	9	0.08978582897820075
1107	10-11	0.06886135560863949
1107	12-13	0.01116475180686649
1107	14-15	0.12361740609438954
1107	16-17	0.11534060474922114
1107	18-19	0.07957536317498182
1107	20-21	0.0729363077034435
1107	22-23	0.05608499471808415
1107	24-25	0.07943117517540799
1107	26-27	0.09269268118185181
1107	28-29	0.1212207651569841
1107	30-31	0.11061900793241364
1107	32-33	0.1362353907983973
1107	34-35	0.1334673534004267
1107	36-37	0.13237383719118867
1107	38-39	0.12298292863904692
1107	40-41	0.1292945830578276
1107	42-43	0.1170860922839978
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2216	74-75	-0.764302801893912
2216	76-77	-0.6952920814681889
2216	78-79	-0.6355055661615125
2216	80-81	-0.6635157354667953
2216	82-83	-0.6845521968075694
2216	84-85	-0.6500994090470797
2216	86-87	-0.6922274251226668
2216	88-89	-0.714337435895068
2216	90-91	-0.7600562838122862
2216	92-93	-0.7007988767790785
2216	94-95	-0.8281144853647149
2216	96-97	-1.0332992683045106
2216	98-99	-1.0535352646354035
2216	100	-1.0909094070115906
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	442.0
3	1060.0
4	653.0
5	537.0
6	853.0
7	1848.0
8	3768.0
9	6694.0
10	9130.0
11	11308.0
12	15050.0
13	21274.0
14	25005.0
15	33009.0
16	42079.0
17	59419.0
18	66579.0
19	37376.0
20	25066.0
21	22587.0
22	23004.0
23	24272.0
24	27565.0
25	33170.0
26	41984.0
27	53678.0
28	68822.0
29	87391.0
30	110164.0
31	138037.0
32	174283.0
33	221282.0
34	295143.0
35	441855.0
36	685788.0
37	1308628.0
38	2067300.0
39	1090339.0
40	6717.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.522433608831626	21.41514065633303	14.9226653230138	24.139760411821545
2	13.887325541018672	21.92846538157412	39.604572686110515	24.579636391296688
3	16.08267958707988	27.253172802105464	31.878972181275362	24.7851754295393
4	11.093551240608972	18.64652145560609	38.69533184995022	31.564595453834716
5	11.533307171399752	38.65182649749837	35.38796508947189	14.426901241629988
6	28.217320014202627	39.654570174092065	17.90016089174504	14.227948919960271
7	24.61618646524125	34.54742646700422	22.873357563661592	17.963029504092937
8	24.859693437971078	35.29147173637154	20.771412514816717	19.077422310840667
9	25.06774876121749	16.146496321170524	22.34154163049303	36.444213287118956
10-11	22.889434014911746	27.503408258256446	29.144274510622452	20.462883216209356
12-13	24.275860241414474	25.60860610073184	29.42046164308647	20.69507201476722
14-15	21.589443536794953	27.085554496338744	28.075159419147656	23.249842547718647
16-17	19.454957663288692	30.29265872130486	28.778370759171946	21.474012856234502
18-19	19.29637949686393	27.89325346322935	32.79419685880811	20.016170181098612
20-21	20.84668891616948	27.023726929482113	32.56636989526111	19.5632142590873
22-23	20.347180392464313	27.100850056960173	32.66566746654851	19.886302084027
24-25	20.11842192162113	27.100527508093723	32.70731422327629	20.073736347008854
26-27	19.93662365465315	27.0447274321486	32.76063312636728	20.258015786830963
28-29	19.84098444568567	27.124273869290157	32.88262701554992	20.15211466947425
30-31	20.26943649179894	27.459421369319347	32.46948162512659	19.801660513755127
32-33	19.84565880461468	27.25060772128871	32.814265312005475	20.08946816209114
34-35	20.109097569662705	27.009694602048434	32.586816296861386	20.294391531427475
36-37	20.301904140025563	27.283904228864515	32.14815261323482	20.266039017875098
38-39	20.274088183431203	27.28577935846312	32.202031907615726	20.238100550489957
40-41	20.206883768188575	27.114687134211856	32.27108741843246	20.40734167916711
42-43	20.17083192222882	27.248973346378314	32.068603810802585	20.51159092059028
44-45	20.48263267115721	27.331193419118467	31.704303270641667	20.48187063908266
46-47	20.504223014662728	27.67236021508071	31.13740369606377	20.686013074192793
48-49	20.55651286811147	27.59824772899527	30.768049956612003	21.077189446281263
50-51	20.60115172002823	27.70157160079198	30.494321037152172	21.20295564202762
52-53	20.626064280992974	27.860415214100176	30.31452601273691	21.19899449216994
54-55	20.781145809312157	28.14168145152515	30.145904807594544	20.931267931568147
56-57	20.617999594516206	27.797067513189038	30.21908139726722	21.365851495027538
58-59	20.56124161937816	27.4406945488625	30.575460784010822	21.422603047748517
60-61	20.675889889764818	27.81068821986348	30.184345464443886	21.32907642592782
62-63	20.488615223818318	27.645756708871865	30.483473762930803	21.382154304379018
64-65	20.478648361384916	27.484010522484265	30.601850510588186	21.435490605542633
66-67	20.460944720217938	27.573930569337364	30.306305608657567	21.658819101787124
68-69	20.86001582980101	27.612505858598507	30.15058769286177	21.376890618738713
70-71	20.775206432508217	27.512906193778473	30.14577796280199	21.566109410911324
72-73	20.653242638256284	27.569870436715714	30.254612044031994	21.522274880996008
74-75	20.71741554436045	27.587258687629777	29.98814522795996	21.707180540049812
76-77	20.780460786315388	27.540624885437758	29.76732349245705	21.9115908357898
78-79	20.82743469910701	27.583879831596725	29.95715773990859	21.631527729387674
80-81	20.54401119074841	27.526585647793766	30.054067198038652	21.87533596341917
82-83	20.491540599369966	27.48992493698752	30.198737320098456	21.819797143544058
84-85	20.523887028574034	27.34348182838225	30.009009879837144	22.123621263206573
86-87	20.6324686856349	27.49269925316748	29.837107771504094	22.037724289693525
88-89	20.390115058589274	27.357689156587135	29.938204012846626	22.31399177197697
90-91	20.327381291552197	27.272815271505124	29.85672837844128	22.5430750585014
92-93	20.34242925046266	27.420489474353744	29.793573224843488	22.443508050340107
94-95	20.168322564425683	27.209676460448	29.96799191120227	22.654009063924047
96-97	20.138660240122167	27.29099427910488	29.833014010742094	22.737331470030863
98-99	20.07982707406361	27.20655493979364	29.85800175144766	22.855616234695088
100	20.209920849920763	27.232784384068047	29.652514593321605	22.904780172689584
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7323.0
1	4327.5
2	4388.5
3	7619.0
4	5021.5
5	2689.0
6	6188.0
7	15498.5
8	25123.0
9	26942.5
10	18334.5
11	9401.0
12	7516.0
13	7797.0
14	8377.5
15	9139.5
16	10015.5
17	11118.0
18	12511.0
19	13855.5
20	15638.0
21	18313.5
22	22409.5
23	28986.0
24	37419.5
25	48373.0
26	64253.0
27	83753.5
28	107332.0
29	132365.5
30	151935.0
31	169429.5
32	186779.5
33	202111.5
34	215315.0
35	226344.0
36	235586.5
37	243407.5
38	247873.0
39	249810.5
40	250856.5
41	248474.5
42	242414.5
43	236535.5
44	231170.5
45	226217.5
46	222317.5
47	217265.5
48	212754.0
49	211889.0
50	212565.5
51	206282.0
52	194586.5
53	183398.5
54	174895.0
55	166943.0
56	150680.0
57	131630.0
58	118766.0
59	107996.5
60	92053.5
61	74664.0
62	62117.5
63	50300.5
64	38905.5
65	29976.0
66	23162.0
67	17607.5
68	13315.0
69	10007.5
70	7183.0
71	5251.0
72	4050.5
73	3034.0
74	2286.5
75	1630.0
76	1053.0
77	770.5
78	543.0
79	302.5
80	161.0
81	82.5
82	41.5
83	23.5
84	9.0
85	5.0
86	4.0
87	2.5
88	4.0
89	2.0
90	0.5
91	1.5
92	2.0
93	1.0
94	2.0
95	2.0
96	0.5
97	1.0
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04806980048080785
2	0.0
3	9.611214035008709E-5
4	0.07141132028011471
5	0.0042975857042253235
6	1.3730305764298157E-5
7	0.0
8	0.0
9	0.0
10-11	0.01162956898236054
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.590546766863115E-4
26-27	0.0
28-29	4.8056070175043553E-4
30-31	0.0040161144360572115
32-33	1.3043790476083248E-4
34-35	0.0018810518897088475
36-37	0.004359372080164665
38-39	0.0013936260350762629
40-41	0.0011533456842010453
42-43	0.002093871629055469
44-45	1.3730305764298157E-5
46-47	4.462349373396901E-4
48-49	5.354819248076281E-4
50-51	0.001908512501237444
52-53	0.0010023123207937655
54-55	0.0012151320601403869
56-57	0.006872018035031228
58-59	0.010078044430994847
60-61	0.0012906487418440269
62-63	0.0028764990576204638
64-65	0.0012082669072582377
66-67	1.0984244611438526E-4
68-69	8.306834987400386E-4
70-71	3.8444856140034837E-4
72-73	0.0
74-75	2.196848922287705E-4
76-77	0.0
78-79	6.384592180398643E-4
80-81	0.0
82-83	0.0010709638496152563
84-85	5.904031478648207E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.6774096240381407E-4
94-95	0.0
96-97	4.531000902218392E-4
98-99	5.629425363362244E-4
100	4.531000902218392E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7283159.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.588350749866045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.96280165437736	35.74051651343972
2	18.289139342101823	19.235913492712495
3	6.408139888797612	10.109805243788891
4	2.9052261438057543	6.11124205832551
5	1.4513229688734333	3.816134066922651
6	0.8290290247402012	2.615836148091424
7	0.5052130256441454	1.859782385718277
8	0.3359673934529719	1.4134376901938506
9	0.23534754394827187	1.113888527034411
>10	0.9977835468074332	8.924125621268301
>50	0.04758635229495014	1.7222392154323691
>100	0.02813197172902322	2.9415590582897533
>500	0.0028480918137190553	1.0058691847019263
>1k	0.0012540442442854336	1.0697471702083228
>5k	5.225184224005255E-5	0.23477529641005718
>10k+	1.5675552672015766E-4	2.0851283274620975
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48698	0.6686384301097916	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42281	0.5805310580202904	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29295	0.4022293073651145	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10730	0.14732618085091922	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10660	0.14636505944741834	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10101	0.13868981852517567	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9412	0.12922963785357425	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7676	0.10539382704675265	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.3730305764298157E-5	0.0	2.7460611528596314E-5	0.0
6	0.0	1.3730305764298157E-5	0.0	4.119091729289447E-5	0.0
7	0.0	1.3730305764298157E-5	0.0	4.119091729289447E-5	0.0
8	1.3730305764298157E-5	1.3730305764298157E-5	0.0	4.119091729289447E-5	0.0
9	1.3730305764298157E-5	1.3730305764298157E-5	0.0	6.865152882149078E-5	0.0
10-11	1.3730305764298157E-5	1.3730305764298157E-5	0.0	1.235727518786834E-4	0.0
12-13	1.3730305764298157E-5	1.3730305764298157E-5	0.0	1.5103336340727973E-4	0.0
14-15	1.3730305764298157E-5	1.3730305764298157E-5	0.0	1.5103336340727973E-4	0.0
16-17	1.3730305764298157E-5	1.3730305764298157E-5	0.0	1.578985162894288E-4	0.0
18-19	1.3730305764298157E-5	1.3730305764298157E-5	0.0	1.6476366917157789E-4	0.0
20-21	8.238183458578894E-5	1.3730305764298157E-5	0.0	2.471455037573668E-4	0.0
22-23	8.238183458578894E-5	1.3730305764298157E-5	0.0	4.8742585463258463E-4	0.0
24-25	8.238183458578894E-5	1.3730305764298157E-5	0.0	0.001324974506254772	0.0
26-27	8.238183458578894E-5	1.3730305764298157E-5	0.0	0.0024851853433379666	0.0
28-29	8.238183458578894E-5	1.3730305764298157E-5	0.0	0.0055745041403050515	0.0
30-31	8.238183458578894E-5	1.3730305764298157E-5	0.0	0.014025507338230568	0.0
32-33	8.238183458578894E-5	1.3730305764298157E-5	0.0	0.03194355636063966	0.0
34-35	1.235727518786834E-4	2.0595458646447236E-5	0.0	0.05976802099198987	0.0
36-37	1.235727518786834E-4	2.7460611528596314E-5	0.0	0.10472104206430205	0.0
38-39	1.235727518786834E-4	2.7460611528596314E-5	0.0	0.1625805505550545	0.0
40-41	1.235727518786834E-4	2.7460611528596314E-5	0.0	0.22367354605329914	0.0
42-43	1.235727518786834E-4	2.7460611528596314E-5	0.0	0.28761557999763565	0.0
44-45	1.235727518786834E-4	2.7460611528596314E-5	0.0	0.3685763279368197	0.0
46-47	1.235727518786834E-4	2.7460611528596314E-5	0.0	0.46683726113901947	0.0
48-49	1.235727518786834E-4	2.7460611528596314E-5	0.0	0.5716818759552003	0.0
50-51	1.235727518786834E-4	2.7460611528596314E-5	0.0	0.6766775241347882	0.0
52-53	1.235727518786834E-4	2.7460611528596314E-5	0.0	0.7850165017679829	0.0
54-55	1.235727518786834E-4	2.7460611528596314E-5	0.0	0.9098043857068066	0.0
56-57	1.235727518786834E-4	2.7460611528596314E-5	0.0	1.0628972949787312	0.0
58-59	1.235727518786834E-4	2.7460611528596314E-5	0.0	1.2296243978746035	0.0
60-61	1.235727518786834E-4	2.7460611528596314E-5	0.0	1.4047063918280513	0.0
62-63	1.235727518786834E-4	2.7460611528596314E-5	0.0	1.5823010317363662	0.0
64-65	1.235727518786834E-4	2.7460611528596314E-5	0.0	1.7748136488575905	0.0
66-67	1.235727518786834E-4	2.7460611528596314E-5	0.0	2.001590244013621	0.0
68-69	1.3730305764298156E-4	2.7460611528596314E-5	0.0	2.25934927412679	0.0
70-71	1.3730305764298156E-4	2.7460611528596314E-5	0.0	2.535149926014247	0.0
72-73	1.3730305764298156E-4	2.7460611528596314E-5	0.0	2.8243925472449525	0.0
74-75	1.3730305764298156E-4	2.7460611528596314E-5	0.0	3.1215644200545394	0.0
76-77	1.3730305764298156E-4	2.7460611528596314E-5	0.0	3.4443845040318357	0.0
78-79	1.3730305764298156E-4	2.7460611528596314E-5	0.0	3.8101667147456206	0.0
80-81	1.3730305764298156E-4	2.7460611528596314E-5	0.0	4.216721068426489	1.3730305764298157E-5
82-83	1.3730305764298156E-4	2.7460611528596314E-5	0.0	4.637589265866639	1.3730305764298157E-5
84-85	1.3730305764298156E-4	2.7460611528596314E-5	0.0	5.062281353462145	1.3730305764298157E-5
86-87	1.3730305764298156E-4	2.7460611528596314E-5	0.0	5.513363088736632	1.3730305764298157E-5
88	1.3730305764298156E-4	2.7460611528596314E-5	0.0	5.876708170177254	1.3730305764298157E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18735	0.0	61.237003	1
GTATCAA	35235	0.0	42.550575	1
GTGGTAT	6490	0.0	38.06699	1
TGGTATC	6660	0.0	36.155632	2
ATCAACG	46235	0.0	32.062366	3
TATCAAC	46350	0.0	32.043697	2
TCAACGC	46560	0.0	31.808044	4
CAACGCA	48895	0.0	30.26424	5
AACGCAG	50845	0.0	29.157818	6
CATGGGT	14685	0.0	26.101227	4
ACGCAGA	57085	0.0	25.863514	7
CGCAGAG	57140	0.0	25.74813	8
GTACATG	30240	0.0	25.443092	1
TACATGG	30350	0.0	24.576767	2
GCAGAGT	60665	0.0	23.934334	9
ACATGGG	30940	0.0	23.560888	3
ATGGGTG	8890	0.0	21.467611	5
GAGTACT	44220	0.0	21.462646	12-13
CAGAGTA	60115	0.0	21.135124	10-11
AGAGTAC	58460	0.0	21.045998	10-11
>>END_MODULE