FastQCFastQC Report
Wed 25 May 2016
SRR2431442_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431442_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529124
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA421992.7596846298926705No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57590.376620862663852No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48620.3179598253640647No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20860.1364179752590372No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16610.10862428423070987No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15900.10398110290597755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG19700.050.3486672
GTACCTG21050.047.5740661
ACCTGGG26000.037.7871973
GGTATCA26950.032.4486731
TCTGTCG4650.032.34438
ATAACGC1807.2759576E-1231.3386633
TATCACG2051.8189894E-1229.809952
TATAACG1751.70985E-1029.5478822
GTATCAA43050.029.3779771
GTACACG2253.6379788E-1227.1646161
TAACCGC700.00883663126.857326
TCAACGC45000.025.279034
TCGCTCT6150.025.2229774
GTACATG65350.025.1805521
ATCAACG45150.025.0917553
CTGTCGC6000.025.0668329
GAACAAA20750.024.4708161
TATCAAC49600.024.3569662
GTCTCGC5850.024.1106071
TACATGG65850.023.2719922
Produced by FastQC (version 0.11.5)