##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431442_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1529124
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.43841245052723	31.0	29.0	34.0	15.0	34.0
2	28.62477470761037	31.0	28.0	34.0	16.0	34.0
3	28.76429642069577	31.0	28.0	34.0	16.0	34.0
4	31.750051663566854	35.0	32.0	37.0	16.0	37.0
5	32.15750586610373	37.0	33.0	37.0	18.0	37.0
6	31.948196483738403	37.0	33.0	37.0	16.0	37.0
7	31.91683539072044	37.0	32.0	37.0	15.0	37.0
8	31.72852038160411	37.0	32.0	37.0	13.0	37.0
9	32.8477252335324	39.0	33.0	39.0	11.0	39.0
10-11	32.88748819585592	38.5	33.0	39.0	10.5	39.0
12-13	32.830026538070165	39.0	33.0	39.0	10.0	39.0
14-15	33.852379205348946	39.5	33.0	41.0	10.0	41.0
16-17	33.82692574310521	39.5	33.0	41.0	9.0	41.0
18-19	33.69153449949121	39.0	33.0	41.0	9.0	41.0
20-21	33.72548890737441	39.0	33.0	41.0	10.0	41.0
22-23	33.65037825578567	39.0	32.5	41.0	10.0	41.0
24-25	33.67589973082627	39.0	32.5	41.0	10.0	41.0
26-27	33.663805551413745	39.0	32.0	41.0	10.0	41.0
28-29	33.57370298288431	39.0	32.0	41.0	9.0	41.0
30-31	33.54441202937106	39.0	32.0	41.0	8.5	41.0
32-33	33.48414451672984	39.0	32.0	41.0	8.0	41.0
34-35	33.37918638383807	39.0	32.0	41.0	4.5	41.0
36-37	33.236677012459424	39.0	32.0	41.0	2.0	41.0
38-39	33.115318966937934	39.0	31.0	41.0	2.0	41.0
40-41	33.022695674124535	39.0	31.0	41.0	2.0	41.0
42-43	32.789336247420096	38.0	31.0	41.0	2.0	41.0
44-45	32.63933860170921	38.0	31.0	40.0	2.0	41.0
46-47	32.4940129119679	38.0	30.0	40.0	2.0	41.0
48-49	32.36013495308425	38.0	30.0	40.0	2.0	41.0
50-51	32.191332423008205	38.0	30.0	40.0	2.0	41.0
52-53	32.08246682414245	38.0	30.0	40.0	2.0	41.0
54-55	32.02320544311645	38.0	30.0	40.0	2.0	41.0
56-57	31.884159165639936	37.0	30.0	40.0	2.0	41.0
58-59	31.680174073521833	37.0	30.0	40.0	2.0	41.0
60-61	31.458434698559437	36.5	30.0	40.0	2.0	41.0
62-63	31.177576507856784	36.0	30.0	40.0	2.0	41.0
64-65	31.027231931484955	36.0	30.0	40.0	2.0	41.0
66-67	30.795336741820805	35.5	29.5	39.0	2.0	41.0
68-69	30.532895958731928	35.0	29.0	39.0	2.0	41.0
70-71	30.234807968483917	35.0	29.0	39.0	2.0	41.0
72-73	29.938143669185756	35.0	29.0	38.0	2.0	40.0
74-75	29.635072433628665	35.0	29.0	37.0	2.0	40.0
76-77	29.3351634661414	35.0	29.0	37.0	2.0	39.0
78-79	29.01484444688593	35.0	28.0	36.5	2.0	39.0
80-81	28.734266155001166	34.0	28.0	36.0	2.0	39.0
82-83	28.449495266570928	34.0	28.0	36.0	2.0	37.5
84-85	28.17161427065431	34.0	28.0	35.0	2.0	37.0
86-87	27.910857458257148	34.0	27.0	35.0	2.0	37.0
88-89	27.645759598305958	34.0	27.0	35.0	2.0	36.0
90-91	27.43535252863731	34.0	27.0	35.0	2.0	36.0
92-93	27.227824231389995	34.0	26.0	35.0	2.0	36.0
94-95	26.989811486838214	34.0	26.0	35.0	2.0	35.5
96-97	26.880783049641494	34.0	26.0	35.0	2.0	35.0
98-99	26.714536231201656	34.0	25.5	35.0	2.0	35.0
100	26.56822860670554	34.0	25.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5147177841152768
1101	2	0.6615244017680517
1101	3	0.4478789797108398
1101	4	0.542955123648909
1101	5	0.49896136703266336
1101	6	0.48281768559607485
1101	7	0.5763214954722748
1101	8	0.7194574583447242
1101	9	0.7551221531423167
1101	10-11	0.8132661841921802
1101	12-13	0.8807333420054917
1101	14-15	0.9316606196475732
1101	16-17	0.9448670264008925
1101	18-19	0.8823126567559143
1101	20-21	0.8934204101445928
1101	22-23	0.7202614432244658
1101	24-25	0.7746019848551526
1101	26-27	0.8077716956153864
1101	28-29	0.8564425731795211
1101	30-31	0.8396866715487477
1101	32-33	0.7386272313323659
1101	34-35	0.6986731947056342
1101	36-37	0.7237899234251657
1101	38-39	0.6918259151362705
1101	40-41	0.5723122513265437
1101	42-43	0.7038552662562481
1101	44-45	0.6179794299146195
1101	46-47	0.5237777284877723
1101	48-49	0.5068027820209124
1101	50-51	0.4080335050456725
1101	52-53	0.33983484362122596
1101	54-55	0.42925168891818544
1101	56-57	0.5558045194436687
1101	58-59	0.6846664055511624
1101	60-61	0.6536861686429027
1101	62-63	0.6878454636561848
1101	64-65	0.6555127951711128
1101	66-67	0.713074065854677
1101	68-69	0.6650372482060654
1101	70-71	0.7793036302355212
1101	72-73	0.6617988023160244
1101	74-75	0.6318374485346041
1101	76-77	0.6439212970270809
1101	78-79	0.5917607665910012
1101	80-81	0.6537060551436298
1101	82-83	0.7517144917280874
1101	84-85	0.6164661861371705
1101	86-87	0.6083886544599402
1101	88-89	0.6621089930660169
1101	90-91	0.6587789838344698
1101	92-93	0.5625418236525093
1101	94-95	0.5439038072507039
1101	96-97	0.6414419533593367
1101	98-99	0.6240178567433823
1101	100	0.5521648336203917
1102	1	0.2924452987057009
1102	2	0.38935304899322887
1102	3	0.24338401265613996
1102	4	0.35301018615995616
1102	5	0.33804077666509613
1102	6	0.42345481696529674
1102	7	0.4644565775415508
1102	8	0.3127991580666638
1102	9	0.5933716914682492
1102	10-11	0.5605624406351026
1102	12-13	0.5414266905996641
1102	14-15	0.5198803384387887
1102	16-17	0.5496200910454121
1102	18-19	0.46778696923588114
1102	20-21	0.5428822627117427
1102	22-23	0.5560756138057386
1102	24-25	0.6612028412891036
1102	26-27	0.660361257847697
1102	28-29	0.6627347699360016
1102	30-31	0.6584774830715219
1102	32-33	0.5970724196437871
1102	34-35	0.5312665383087349
1102	36-37	0.5127931346295327
1102	38-39	0.37967703487037596
1102	40-41	0.5560357552952908
1102	42-43	0.5690610295052139
1102	44-45	0.5151924454436099
1102	46-47	0.4436030452304536
1102	48-49	0.49952068245496406
1102	50-51	0.37220300774042414
1102	52-53	0.21551445408081094
1102	54-55	0.33633052923013906
1102	56-57	0.3361277082135814
1102	58-59	0.34158684280281904
1102	60-61	0.34375482547800473
1102	62-63	0.36861444389577613
1102	64-65	0.36610300201294166
1102	66-67	0.4376073372648932
1102	68-69	0.47758573138573013
1102	70-71	0.3743457836373629
1102	72-73	0.4273104590047758
1102	74-75	0.49504234215288534
1102	76-77	0.5201942316330523
1102	78-79	0.4824071399011203
1102	80-81	0.4311772486271579
1102	82-83	0.42538377913003345
1102	84-85	0.44060378827436253
1102	86-87	0.43033906774600084
1102	88-89	0.42527992202277076
1102	90-91	0.29909512165908936
1102	92-93	0.44748219727224736
1102	94-95	0.5268302202525277
1102	96-97	0.4643068323766215
1102	98-99	0.36095838094119514
1102	100	0.39707808626756247
1103	1	0.47531086534422684
1103	2	0.4304119702913063
1103	3	0.3948745001154492
1103	4	0.3644138492672795
1103	5	0.34738284097419125
1103	6	0.3496508175667614
1103	7	0.40262181442740186
1103	8	0.5276090897956713
1103	9	0.6103362053609871
1103	10-11	0.5687684082777977
1103	12-13	0.6486379754965341
1103	14-15	0.7458041337442012
1103	16-17	0.665577237017132
1103	18-19	0.446907789651128
1103	20-21	0.4956489613010504
1103	22-23	0.4927095540954909
1103	24-25	0.6796000580585329
1103	26-27	0.6380196735126304
1103	28-29	0.6724637836543437
1103	30-31	0.6062262151212252
1103	32-33	0.6286976958557418
1103	34-35	0.5833219595297692
1103	36-37	0.6976381042562707
1103	38-39	0.7059573764171745
1103	40-41	0.6882649902307136
1103	42-43	0.7351001753370809
1103	44-45	0.600943095326798
1103	46-47	0.5756667540992879
1103	48-49	0.49517977425004744
1103	50-51	0.46437256253615544
1103	52-53	0.49246153049858066
1103	54-55	0.6188866188464601
1103	56-57	0.630299086786863
1103	58-59	0.6650317898643934
1103	60-61	0.7408321611126816
1103	62-63	0.648274188307024
1103	64-65	0.6151013372641785
1103	66-67	0.5969529389846322
1103	68-69	0.6138915484770529
1103	70-71	0.6248242622578921
1103	72-73	0.6219279638452555
1103	74-75	0.607440316732621
1103	76-77	0.5887385242427108
1103	78-79	0.6565603632041395
1103	80-81	0.5084894724538742
1103	82-83	0.4539409961799805
1103	84-85	0.46968718226620965
1103	86-87	0.35995872494099856
1103	88-89	0.4971580436845322
1103	90-91	0.49203024224766523
1103	92-93	0.4968333041517603
1103	94-95	0.5399248275267219
1103	96-97	0.47334373056045465
1103	98-99	0.47921082170297424
1103	100	0.6267663877927347
1104	1	0.3331054219609513
1104	2	0.4282461208755386
1104	3	0.3736642433127173
1104	4	0.4109249024337345
1104	5	0.29041683586121536
1104	6	0.17515056036342003
1104	7	0.4014502985660009
1104	8	0.3270114134150681
1104	9	0.4600852103796953
1104	10-11	0.4990611218198495
1104	12-13	0.37853032478842863
1104	14-15	0.5231298209335193
1104	16-17	0.5138720117733726
1104	18-19	0.4569494823022069
1104	20-21	0.4486494111328412
1104	22-23	0.468238664510757
1104	24-25	0.437765342244802
1104	26-27	0.5443083698594222
1104	28-29	0.5345736960776577
1104	30-31	0.46983450390486325
1104	32-33	0.46105503171148143
1104	34-35	0.4469210802750325
1104	36-37	0.4648426756793711
1104	38-39	0.5115864921937501
1104	40-41	0.5856146498473649
1104	42-43	0.39775572555790006
1104	44-45	0.39648378138705453
1104	46-47	0.30023018108014554
1104	48-49	0.33968306687130934
1104	50-51	0.4068579699089696
1104	52-53	0.4293790486468154
1104	54-55	0.2714231460054819
1104	56-57	0.371725525934707
1104	58-59	0.41967565043766086
1104	60-61	0.38552254150854637
1104	62-63	0.3854273250417428
1104	64-65	0.4118683481109642
1104	66-67	0.4489394436321277
1104	68-69	0.391389833954225
1104	70-71	0.2280340522878852
1104	72-73	0.17520572690766656
1104	74-75	0.3668096035250059
1104	76-77	0.24217611013078866
1104	78-79	0.3875361354668776
1104	80-81	0.2924044879269303
1104	82-83	0.3285295057180271
1104	84-85	0.23967544917013228
1104	86-87	0.22940685487044377
1104	88-89	0.347841092439964
1104	90-91	0.29876360838663274
1104	92-93	0.2913239508592085
1104	94-95	0.22222559060647384
1104	96-97	0.18205661570028298
1104	98-99	0.30767353499190975
1104	100	0.21612117911920947
1105	1	0.29325849802964754
1105	2	0.46642536359227194
1105	3	0.29341495219307134
1105	4	0.31835430833726264
1105	5	0.2943364079864992
1105	6	0.3289606559162621
1105	7	0.46920530194750043
1105	8	0.36004204272243
1105	9	0.3712503920996042
1105	10-11	0.36269825770961717
1105	12-13	0.3099492033336233
1105	14-15	0.455270852938213
1105	16-17	0.4862348002027801
1105	18-19	0.4439788107487246
1105	20-21	0.47876739021520365
1105	22-23	0.36876653832145934
1105	24-25	0.4665565899881372
1105	26-27	0.4605432986103608
1105	28-29	0.5156735663970053
1105	30-31	0.5647379789872389
1105	32-33	0.512839255617088
1105	34-35	0.5463091273755367
1105	36-37	0.4976323619263354
1105	38-39	0.42765799281917793
1105	40-41	0.42332492238137576
1105	42-43	0.431712962412071
1105	44-45	0.3912771524410985
1105	46-47	0.407195192402348
1105	48-49	0.30516314688448887
1105	50-51	0.26372031979498445
1105	52-53	0.38572636343486266
1105	54-55	0.3974335671789717
1105	56-57	0.3005609272661047
1105	58-59	0.3067373556079147
1105	60-61	0.3308059749162702
1105	62-63	0.3272823394857767
1105	64-65	0.3566671925109759
1105	66-67	0.36102833694860337
1105	68-69	0.3992775336372354
1105	70-71	0.2950199878159232
1105	72-73	0.4392148379532621
1105	74-75	0.5302302960934355
1105	76-77	0.47111265735108887
1105	78-79	0.47157970059785725
1105	80-81	0.4103792263598791
1105	82-83	0.30829656320283405
1105	84-85	0.3274918582806343
1105	86-87	0.39203618076851754
1105	88-89	0.43785803761016595
1105	90-91	0.3638651490900813
1105	92-93	0.4315222114742667
1105	94-95	0.4672193131516593
1105	96-97	0.5865797424693433
1105	98-99	0.6134984300088853
1105	100	0.5409226569033159
1106	1	0.29899971692202953
1106	2	0.34106710522944894
1106	3	0.4004548935738619
1106	4	0.4655670221195649
1106	5	0.3021670115292352
1106	6	0.510649236184122
1106	7	0.48295344923864647
1106	8	0.4266739264837476
1106	9	0.692909759176807
1106	10-11	0.659798779935258
1106	12-13	0.6623888459077563
1106	14-15	0.5975560193640277
1106	16-17	0.7061416539554912
1106	18-19	0.6247677819719897
1106	20-21	0.6589234645972155
1106	22-23	0.6602645282922666
1106	24-25	0.7503164959778772
1106	26-27	0.6709618912228095
1106	28-29	0.7543003350688124
1106	30-31	0.7152895550273826
1106	32-33	0.6665744130876092
1106	34-35	0.6187664061434646
1106	36-37	0.6903575617704405
1106	38-39	0.7362102674839832
1106	40-41	0.744690414698546
1106	42-43	0.6285537067662474
1106	44-45	0.5235204236361497
1106	46-47	0.6374171429371955
1106	48-49	0.635750373548511
1106	50-51	0.6068808436448165
1106	52-53	0.7148719350852346
1106	54-55	0.8767496047311596
1106	56-57	0.8195183523224827
1106	58-59	0.7498537704322601
1106	60-61	0.9102613665817962
1106	62-63	0.9225366978246079
1106	64-65	0.9275203971549786
1106	66-67	1.00819377119538
1106	68-69	0.9912725705838703
1106	70-71	0.9684577329046924
1106	72-73	0.9517633829116541
1106	74-75	1.0536863226026085
1106	76-77	1.095791295566972
1106	78-79	0.9904875284944197
1106	80-81	0.9598896143227691
1106	82-83	0.9731193279839907
1106	84-85	1.0659270057761
1106	86-87	0.9662465105497695
1106	88-89	1.1161261752451601
1106	90-91	1.1817945316341785
1106	92-93	1.1813946494333152
1106	94-95	1.1986829540763786
1106	96-97	1.1766994505450938
1106	98-99	1.173045707134083
1106	100	1.03438565853455
1107	1	0.15977444121681827
1107	2	0.22475566522023982
1107	3	0.1941533662754047
1107	4	0.14463620078119277
1107	5	0.33264505554690516
1107	6	0.171716801351387
1107	7	0.1866549331667251
1107	8	0.17010543887862895
1107	9	0.19629112320794206
1107	10-11	0.22687430505647654
1107	12-13	0.14789554743054367
1107	14-15	0.16872984559410042
1107	16-17	0.31881664744448557
1107	18-19	0.23306478262326635
1107	20-21	0.3573087170698628
1107	22-23	0.48799076893394755
1107	24-25	0.5447563333789134
1107	26-27	0.5357485572647107
1107	28-29	0.5871344913133285
1107	30-31	0.5274863664690201
1107	32-33	0.5587086643909842
1107	34-35	0.5365299547558848
1107	36-37	0.49758612185566164
1107	38-39	0.6148408568235197
1107	40-41	0.5017167612961728
1107	42-43	0.5872609461689322
1107	44-45	0.5085400256675072
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2216	88-89	-1.5946977567109677
2216	90-91	-1.80442671044651
2216	92-93	-1.6464502576286755
2216	94-95	-1.6224551938262834
2216	96-97	-1.6823756112535584
2216	98-99	-1.9000019238113985
2216	100	-1.7937718566100216
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83114.0
3	17585.0
4	13973.0
5	13138.0
6	8212.0
7	6040.0
8	5676.0
9	5841.0
10	5943.0
11	6192.0
12	6856.0
13	7340.0
14	7388.0
15	7071.0
16	7232.0
17	7092.0
18	6834.0
19	6607.0
20	6486.0
21	7016.0
22	8778.0
23	15086.0
24	25003.0
25	25250.0
26	28942.0
27	22122.0
28	22369.0
29	24214.0
30	27632.0
31	31605.0
32	39693.0
33	50813.0
34	67967.0
35	108220.0
36	153852.0
37	248364.0
38	287336.0
39	105985.0
40	257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.16768480123484	23.316088270304917	12.133569662642092	21.382657265818146
2	15.801927514165973	25.83916715063806	36.804585432722746	21.554319902473225
3	16.850622764861463	32.04465960491515	28.190737926000725	22.913979704222662
4	11.963215462010762	22.78203179569552	35.891346830502314	29.363405911791407
5	11.257532730671278	41.45202662665779	31.41850131524388	15.871939327427054
6	25.69043989198091	42.72306733403576	18.010855539183083	13.57563723480025
7	23.632422901496255	37.964366671942386	20.978366507130723	17.42484391943064
8	22.910009378519398	39.15943875382106	20.278268649453963	17.65228321820558
9	23.188007709367064	23.767910642497394	20.451012361278153	32.593069286857386
10-11	21.112112820120423	33.117709746585064	26.37478021534237	19.39539721795215
12-13	22.215696744938604	31.36131302485629	26.0467368780409	20.376253352164202
14-15	20.345891136637725	33.22930264558846	24.05901496106043	22.36579125671338
16-17	19.291574303668284	35.814040788938875	24.280467854079596	20.613917053313248
18-19	19.15132743449646	34.145358074919955	26.553660307960747	20.149654182622836
20-21	21.384908071512797	33.566340040900705	25.457745857119598	19.591006030466904
22-23	20.68769881547831	34.21336432413201	25.41591020865161	19.683026651738068
24-25	20.21366798894349	33.55449396996641	26.11604099134465	20.11579704974545
26-27	20.161172180337047	33.05148039826088	26.297931785006096	20.48941563639598
28-29	20.190509641251648	33.18159015082095	26.30448446888905	20.323415739038353
30-31	20.638769861823967	34.0674425661712	25.5298666373677	19.763920934637138
32-33	19.655443032179797	33.41306615480703	26.52670095777485	20.40478985523833
34-35	19.74007033698643	32.48947479427821	26.79724734192572	20.973207526809638
36-37	20.7304232380342	33.381882155201446	25.855583060014588	20.032111546749768
38-39	20.49976325385988	33.144394217639025	26.208549805632725	20.147292722868368
40-41	19.89633537608428	32.827239905680955	26.657098731218515	20.61932598701625
42-43	19.816508301500072	32.93334929083928	26.56581392109275	20.6843284865679
44-45	20.451126885281496	33.14344816039951	26.234695065405255	20.17072988891374
46-47	20.04736917315189	33.84364902863111	25.95811722742206	20.15086457079494
48-49	19.759706203671275	32.97174489474598	26.73351049793455	20.535038403648194
50-51	19.85211335077087	32.48637331094945	26.806376266982063	20.855137071297612
52-53	19.94066401628682	32.76025790519715	26.42079695818883	20.878281120327202
54-55	20.307913357594323	33.436021258722235	26.050927150650416	20.20513823303303
56-57	19.680595384851824	33.08993228020496	26.135851598494536	21.093620736448685
58-59	19.577678576099867	32.394695951071746	26.79779425229024	21.22983122053815
60-61	20.425731450029623	33.1212240514922	25.915579633846626	20.537464864631552
62-63	20.107122887216867	32.957945437957996	26.140273431496613	20.794658243328527
64-65	19.979680439430936	32.40662534085295	26.717671575864653	20.89602264385147
66-67	19.86979854711162	32.61847553973208	26.1156294062097	21.39609650694661
68-69	20.688372007853058	32.876568093619326	26.07750643194693	20.35755346658069
70-71	20.685874420399326	32.44759886728534	26.282675090087437	20.58385162222789
72-73	20.13889933444205	32.57630503834626	26.938027968213834	20.346767658997848
74-75	20.052240187143866	32.425244802304114	26.64982005649108	20.87269495406094
76-77	20.151102202442946	32.619796971743135	26.09532747257778	21.13377335323614
78-79	20.51966975053406	32.76802849282889	26.346024243886788	20.366277512750262
80-81	19.975540808101616	32.41761781204681	26.656170141801184	20.950671238050393
82-83	19.749520635560078	32.58445446626399	26.718289355624325	20.947735542551605
84-85	20.02450466664465	32.40890944423717	26.530503470513473	21.036082418604714
86-87	20.525119522961063	32.57155600404029	26.28177564638534	20.621548826613314
88-89	20.017134177943667	32.37764512959357	26.603198270886775	21.002022421575987
90-91	19.823112097018907	32.14336631125156	26.49787064843202	21.53565094329751
92-93	20.113544174615534	32.60595390807578	26.200308482819757	21.08019343448893
94-95	20.059420399325226	32.310643255578675	26.522484238323802	21.1074521067723
96-97	19.887156765770992	32.42410221615479	26.546575219285696	21.14216579878851
98-99	19.897051124887476	32.39377715839	26.56319025105151	21.14598146567101
100	20.215330405233956	32.68246776518352	26.190314251856655	20.911887577725867
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	35794.0
1	27434.5
2	15942.0
3	10044.5
4	5672.0
5	3439.0
6	2595.5
7	2509.0
8	2764.0
9	2763.5
10	2266.0
11	1711.5
12	1541.0
13	1479.0
14	1446.5
15	1521.0
16	1624.0
17	1759.5
18	1985.5
19	2280.0
20	2634.0
21	3320.5
22	4426.5
23	6201.0
24	8267.5
25	10559.5
26	13668.0
27	17418.0
28	21847.0
29	26267.5
30	29747.5
31	32576.0
32	35501.5
33	38099.5
34	40363.5
35	42684.5
36	43799.5
37	44194.5
38	44695.0
39	44541.0
40	44292.5
41	43907.0
42	42562.0
43	41514.5
44	41019.5
45	40932.0
46	41520.5
47	42062.0
48	42832.5
49	43978.0
50	45129.5
51	44148.5
52	41700.5
53	39599.5
54	38346.5
55	37557.0
56	34972.5
57	31870.0
58	29677.0
59	27459.5
60	24191.0
61	20530.0
62	16762.5
63	12897.0
64	9818.5
65	7394.5
66	5551.0
67	4203.5
68	3085.5
69	2224.0
70	1650.5
71	1277.0
72	1033.0
73	840.0
74	658.5
75	506.5
76	384.0
77	317.0
78	251.0
79	188.0
80	142.0
81	96.0
82	75.0
83	64.5
84	54.0
85	49.5
86	44.5
87	33.5
88	26.5
89	21.0
90	25.0
91	26.0
92	15.5
93	9.5
94	13.5
95	13.0
96	9.5
97	10.0
98	6.5
99	14.0
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012033033292264067
2	0.007062867367198475
3	0.008893981129064745
4	0.007324455047465084
5	0.00850159960866483
6	0.008828584208998094
7	0.006932073527065169
8	0.006408898166531949
9	0.00490476900499894
10-11	0.004610482864699004
12-13	0.004872070544965614
14-15	0.0057549289658654235
16-17	0.003760322903832521
18-19	0.004283498264365741
20-21	0.0035968306036658895
22-23	0.004446990564532373
24-25	0.004545085944632351
26-27	0.004970165925065593
28-29	0.014714307014996821
30-31	0.003923815203999152
32-33	0.0038584182839325
34-35	0.0036949259837658686
36-37	0.005166356685265551
38-39	0.003923815203999152
40-41	0.0038257198238991733
42-43	0.00412000596419911
44-45	0.0027466706427994067
46-47	0.004610482864699004
48-49	0.00245238450249947
50-51	0.0031063537031659955
52-53	0.0022888922023328387
54-55	0.003760322903832521
56-57	0.0018965106819329237
58-59	0.001831113761866271
60-61	0.0024850829625327965
62-63	0.002550479882599449
64-65	0.003629529063699216
66-67	0.0020927014421328813
68-69	0.0024850829625327965
70-71	0.0035314336835992376
72-73	0.002027304522066229
74-75	0.0021580983621995336
76-77	0.0017330183817662922
78-79	0.00245238450249947
80-81	0.0030409567830993432
82-83	0.0010463507210664406
84-85	0.0017984153018329447
86-87	0.0016022245416329873
88-89	0.0010790491810997668
90-91	0.0013079384013330508
92-93	0.0017984153018329447
94-95	0.0012752399412997244
96-97	7.520645807665042E-4
98-99	0.0010136522610331145
100	0.0020927014421328813
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1529124.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.37213253479977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.06742485193062	64.01017928016705
2	10.124449270835497	15.05953766024869
3	2.129024459119477	4.750202677303925
4	0.6883306543740177	2.0477047461947966
5	0.29944794842249844	1.1135291253675972
6	0.16993438460690025	0.7583029544522415
7	0.1095926626536097	0.5705448022201105
8	0.0719687246802998	0.4281974024227017
9	0.048388391559317775	0.3238873083175831
>10	0.2515252353993022	3.3967494400571465
>50	0.025646198470152252	1.3411266292200237
>100	0.01259402107205519	1.6652108259116247
>500	7.925669413712952E-4	0.4099944821953961
>1k	7.045039478855957E-4	0.9838557686164922
>5k	8.806299348569947E-5	0.3771818247545186
>10k+	8.806299348569947E-5	2.7637950725500833
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42199	2.7596846298926705	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5759	0.376620862663852	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4862	0.3179598253640647	No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2086	0.1364179752590372	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1661	0.10862428423070987	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1590	0.10398110290597755	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	6.539692006665254E-5	0.0
20-21	0.0	0.0	0.0	2.6158768026661016E-4	0.0
22-23	0.0	0.0	0.0	7.193661207331779E-4	6.539692006665254E-5
24-25	0.0	0.0	0.0	0.002517781422566123	6.539692006665254E-5
26-27	0.0	0.0	0.0	0.005231753605332203	6.539692006665254E-5
28-29	0.0	0.0	0.0	0.008730488828898115	1.3079384013330508E-4
30-31	0.0	0.0	0.0	0.016937802297263008	1.9619076019995762E-4
32-33	0.0	0.0	0.0	0.0334832230741261	1.9619076019995762E-4
34-35	0.0	0.0	0.0	0.05895532344008726	1.9619076019995762E-4
36-37	0.0	0.0	0.0	0.0914902911732469	1.9619076019995762E-4
38-39	0.0	0.0	0.0	0.13527352915787078	1.9619076019995762E-4
40-41	0.0	3.269846003332627E-5	0.0	0.19197265885565853	1.9619076019995762E-4
42-43	0.0	6.539692006665254E-5	0.0	0.2522686191571122	1.9619076019995762E-4
44-45	0.0	6.539692006665254E-5	0.0	0.3160633146821318	1.9619076019995762E-4
46-47	0.0	6.539692006665254E-5	0.0	0.39055040663804896	1.9619076019995762E-4
48-49	6.539692006665254E-5	6.539692006665254E-5	0.0	0.47321211360229776	1.9619076019995762E-4
50-51	6.539692006665254E-5	6.539692006665254E-5	0.0	0.5609747803317455	2.6158768026661016E-4
52-53	6.539692006665254E-5	6.539692006665254E-5	0.0	0.6481815732406266	2.6158768026661016E-4
54-55	6.539692006665254E-5	6.539692006665254E-5	0.0	0.7446747287989725	2.6158768026661016E-4
56-57	6.539692006665254E-5	6.539692006665254E-5	0.0	0.8608523572973807	2.6158768026661016E-4
58-59	6.539692006665254E-5	6.539692006665254E-5	0.0	0.9844198377633208	2.6158768026661016E-4
60-61	6.539692006665254E-5	6.539692006665254E-5	0.0	1.1137422471951262	2.6158768026661016E-4
62-63	6.539692006665254E-5	6.539692006665254E-5	0.0	1.2391408414229323	2.6158768026661016E-4
64-65	6.539692006665254E-5	6.539692006665254E-5	0.0	1.3854991485321007	2.6158768026661016E-4
66-67	6.539692006665254E-5	6.539692006665254E-5	0.0	1.5531768515829978	2.6158768026661016E-4
68-69	6.539692006665254E-5	6.539692006665254E-5	0.0	1.7338685417271589	2.6158768026661016E-4
70-71	6.539692006665254E-5	6.539692006665254E-5	0.0	1.9329694648700824	2.6158768026661016E-4
72-73	6.539692006665254E-5	6.539692006665254E-5	0.0	2.1581637591196006	2.6158768026661016E-4
74-75	6.539692006665254E-5	6.539692006665254E-5	0.0	2.379270745864953	2.6158768026661016E-4
76-77	6.539692006665254E-5	6.539692006665254E-5	0.0	2.613489815083669	2.6158768026661016E-4
78-79	6.539692006665254E-5	6.539692006665254E-5	0.0	2.8746524153698454	2.6158768026661016E-4
80-81	6.539692006665254E-5	6.539692006665254E-5	0.0	3.1588347315194842	2.6158768026661016E-4
82-83	6.539692006665254E-5	6.539692006665254E-5	0.0	3.4611319945275856	2.6158768026661016E-4
84-85	6.539692006665254E-5	6.539692006665254E-5	0.0	3.7676473588799864	2.6158768026661016E-4
86-87	6.539692006665254E-5	6.539692006665254E-5	0.0	4.101465937360214	2.6158768026661016E-4
88	6.539692006665254E-5	6.539692006665254E-5	0.0	4.372634266416589	2.6158768026661016E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	1970	0.0	50.348667	2
GTACCTG	2105	0.0	47.574066	1
ACCTGGG	2600	0.0	37.787197	3
GGTATCA	2695	0.0	32.448673	1
TCTGTCG	465	0.0	32.3443	8
ATAACGC	180	7.2759576E-12	31.338663	3
TATCACG	205	1.8189894E-12	29.80995	2
TATAACG	175	1.70985E-10	29.547882	2
GTATCAA	4305	0.0	29.377977	1
GTACACG	225	3.6379788E-12	27.164616	1
TAACCGC	70	0.008836631	26.85732	6
TCAACGC	4500	0.0	25.27903	4
TCGCTCT	615	0.0	25.222977	4
GTACATG	6535	0.0	25.180552	1
ATCAACG	4515	0.0	25.091755	3
CTGTCGC	600	0.0	25.066832	9
GAACAAA	2075	0.0	24.470816	1
TATCAAC	4960	0.0	24.356966	2
GTCTCGC	585	0.0	24.110607	1
TACATGG	6585	0.0	23.271992	2
>>END_MODULE