FastQCFastQC Report
Wed 25 May 2016
SRR2431442_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431442_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529124
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280901.83699948467227No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198381.297344100282253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166991.0920631681930308No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138250.9041124199214714No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124630.8150418147906907No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56940.37237006285951957No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41750.27303214127827435No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35340.2311127155155501No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17690.11568715159790835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62600.053.9981041
TATAACG2250.050.1782842
TACCTGG15250.047.5048522
GTACCTG18150.040.724091
ATAACGC2850.039.6144373
TATCACG1800.039.2017862
GTATCAA110700.038.1055951
GCGTATC906.111022E-736.61711
TAACGCA3250.036.1862644
ACCTGGG20250.034.381423
CGCGAGT550.00269496834.2393651
TCTGTCG5250.032.2289858
CTGTCGC5900.030.2715769
TATCAAC141950.029.6602442
ATCAACG143100.029.3232673
TCAACGC144250.029.0894934
CAACGCA150600.027.9631755
CGTATCA1205.8042515E-627.441252
AACGCAG154750.027.3955086
GTATAAC5700.027.2563381
Produced by FastQC (version 0.11.5)