FastQCFastQC Report
Wed 25 May 2016
SRR2431441_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431441_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3522182
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA363371.031661623391409No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49900.14167354213950328No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37320.10595704594481489No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC3600.052.2292183
TACCTGG45050.051.0242082
GTACCTG48950.047.633191
TATAACG4100.047.0069662
GGTATCA56900.036.9297181
TCTGTCG15000.036.0335548
GTCTCGC13500.035.5178871
ACCTGGG71100.031.6019823
GTATCAA87600.029.8904041
TCGCTCT15650.029.7351914
CTGTCGC18750.029.578859
GTACACG3550.029.1323181
TAACGCA7000.028.8748874
TCTCGCT16850.028.4552562
GTATAAC10650.027.8081191
CTCGCTC17050.027.0182823
GTACATG144900.026.083651
TCAACGC96500.025.8165654
CGCTCTG16050.025.4796895
ATCAACG99350.025.0763343
Produced by FastQC (version 0.11.5)