##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431441_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3522182
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.395586315528274	31.0	30.0	34.0	23.0	34.0
2	29.42441446807689	31.0	30.0	34.0	22.0	34.0
3	29.533622339788234	31.0	30.0	34.0	22.0	34.0
4	32.719186572414486	37.0	35.0	37.0	22.0	37.0
5	32.91890935789235	37.0	35.0	37.0	25.0	37.0
6	32.883184628165154	37.0	35.0	37.0	23.0	37.0
7	32.742731636241395	37.0	35.0	37.0	22.0	37.0
8	32.645411281983726	37.0	35.0	37.0	19.0	37.0
9	33.89290701048384	39.0	35.0	39.0	17.0	39.0
10-11	33.84187742143932	39.0	35.0	39.0	17.0	39.0
12-13	33.80806812368015	39.0	35.0	39.0	17.0	39.0
14-15	34.9460083266566	40.0	35.5	41.0	17.0	41.0
16-17	34.95857241335059	40.0	35.5	41.0	17.0	41.0
18-19	34.95491857036349	40.0	35.0	41.0	17.5	41.0
20-21	35.05998937590391	40.0	35.0	41.0	18.5	41.0
22-23	35.02799145529674	40.0	35.0	41.0	21.0	41.0
24-25	35.078896547651425	40.0	35.0	41.0	23.0	41.0
26-27	35.08020156255412	40.0	35.0	41.0	24.0	41.0
28-29	34.96758188531995	40.0	34.0	41.0	23.5	41.0
30-31	34.95049276272492	40.0	34.0	41.0	24.0	41.0
32-33	34.87841741284238	40.0	34.0	41.0	24.0	41.0
34-35	34.77655512974627	40.0	34.0	41.0	23.5	41.0
36-37	34.64415311304186	40.0	34.0	41.0	22.5	41.0
38-39	34.51692885262601	39.5	34.0	41.0	21.5	41.0
40-41	34.42383769492888	39.0	34.0	41.0	20.5	41.0
42-43	34.211766172219384	39.0	33.0	41.0	18.0	41.0
44-45	34.0912137703276	39.0	33.0	41.0	18.0	41.0
46-47	33.95956483793285	39.0	33.0	41.0	16.0	41.0
48-49	33.829971023643864	39.0	33.0	41.0	15.0	41.0
50-51	33.6703824504242	39.0	33.0	41.0	14.0	41.0
52-53	33.55943418028937	38.0	33.0	40.5	12.0	41.0
54-55	33.479181796965634	38.0	33.0	41.0	9.0	41.0
56-57	33.33143559872829	38.0	32.5	40.0	7.5	41.0
58-59	33.11987327741724	38.0	32.0	40.0	6.0	41.0
60-61	32.88121610410819	37.5	32.0	40.0	2.0	41.0
62-63	32.55769193641896	37.0	31.5	40.0	2.0	41.0
64-65	32.373266060640816	37.0	31.0	40.0	2.0	41.0
66-67	32.09780017046251	36.0	31.0	39.5	2.0	41.0
68-69	31.80320437728658	36.0	31.0	39.0	2.0	41.0
70-71	31.466300009482758	35.0	31.0	39.0	2.0	41.0
72-73	31.13700640682395	35.0	31.0	38.5	2.0	40.5
74-75	30.816986458962084	35.0	30.0	37.5	2.0	40.0
76-77	30.50601970596636	35.0	30.0	37.0	2.0	39.5
78-79	30.17786886083683	35.0	30.0	36.5	2.0	39.0
80-81	29.887897615739334	35.0	30.0	36.0	2.0	39.0
82-83	29.60617878349273	35.0	30.0	36.0	2.0	37.5
84-85	29.328652096910382	35.0	29.0	35.0	2.0	37.0
86-87	29.08188304863292	34.5	29.0	35.0	2.0	37.0
88-89	28.83328714984064	34.0	29.0	35.0	2.0	36.0
90-91	28.63174319214623	34.0	29.0	35.0	2.0	36.0
92-93	28.437688342056145	34.0	29.0	35.0	2.0	36.0
94-95	28.211541879437235	34.0	29.0	35.0	2.0	35.5
96-97	28.11455398954398	34.0	29.0	35.0	2.0	35.0
98-99	27.957916853813913	34.0	28.5	35.0	2.0	35.0
100	27.819210932314117	34.0	28.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4782543605015448
1101	2	0.4961306032009567
1101	3	0.4877270367844595
1101	4	0.4516757682252788
1101	5	0.4096032760010644
1101	6	0.403418107845134
1101	7	0.4844650810306348
1101	8	0.482200412119866
1101	9	0.49942132590088306
1101	10-11	0.5606696450622977
1101	12-13	0.5103111984378828
1101	14-15	0.6529297401141321
1101	16-17	0.6202879807720194
1101	18-19	0.6109484368423708
1101	20-21	0.633283292696575
1101	22-23	0.5181267121626334
1101	24-25	0.5472036696520917
1101	26-27	0.7014568556322658
1101	28-29	0.7861758227398283
1101	30-31	0.8232258227380953
1101	32-33	0.7333635508758007
1101	34-35	0.7550295422958513
1101	36-37	0.7805077637567024
1101	38-39	0.7501178609974559
1101	40-41	0.751324636628425
1101	42-43	0.7538751886694257
1101	44-45	0.694499713134924
1101	46-47	0.7044066415485588
1101	48-49	0.6556286782707588
1101	50-51	0.700345921288438
1101	52-53	0.6688440186739157
1101	54-55	0.7231013410950453
1101	56-57	0.6864501479179594
1101	58-59	0.7162279981651523
1101	60-61	0.6885096088378191
1101	62-63	0.7514861491463805
1101	64-65	0.7214458394069467
1101	66-67	0.7467190722980916
1101	68-69	0.7211010979488037
1101	70-71	0.6818167051425235
1101	72-73	0.6383078523546075
1101	74-75	0.6279430712824912
1101	76-77	0.6709962840108936
1101	78-79	0.5957061631354925
1101	80-81	0.5917194011037097
1101	82-83	0.5552339146759593
1101	84-85	0.5129253153957372
1101	86-87	0.4958252121982909
1101	88-89	0.5234750703303703
1101	90-91	0.5430387163940118
1101	92-93	0.5770297523826002
1101	94-95	0.5463806381586025
1101	96-97	0.6478004143399332
1101	98-99	0.7061825695012232
1101	100	0.677896524351727
1102	1	0.5414349897099768
1102	2	0.5380357263758135
1102	3	0.5365827776217031
1102	4	0.5874806451525956
1102	5	0.5112792408111702
1102	6	0.37351729655657806
1102	7	0.5696949546371926
1102	8	0.5406824135707851
1102	9	0.681496983157011
1102	10-11	0.6834401980463909
1102	12-13	0.7295635280826076
1102	14-15	0.8392022063700466
1102	16-17	0.737790139269606
1102	18-19	0.7349586571368363
1102	20-21	0.7294631989999019
1102	22-23	0.6898963736489065
1102	24-25	0.7366634960526852
1102	26-27	0.7771686489478213
1102	28-29	0.8649908714273664
1102	30-31	0.7946658343863149
1102	32-33	0.9133425917365443
1102	34-35	0.8864800654441396
1102	36-37	0.7984752125913701
1102	38-39	0.7027955474967555
1102	40-41	0.7730575781114268
1102	42-43	0.8407130791148205
1102	44-45	0.737205332381059
1102	46-47	0.7137773948003598
1102	48-49	0.675733359017471
1102	50-51	0.6814334592218003
1102	52-53	0.642286771230907
1102	54-55	0.7345140458330093
1102	56-57	0.7798643035750175
1102	58-59	0.7555118878544249
1102	60-61	0.753017714060114
1102	62-63	0.7440599595459929
1102	64-65	0.726221291797394
1102	66-67	0.690084949133464
1102	68-69	0.7030280232264658
1102	70-71	0.6971868683198323
1102	72-73	0.693821664184707
1102	74-75	0.6768537324724484
1102	76-77	0.6443710885966176
1102	78-79	0.6540027764655569
1102	80-81	0.6594803877286886
1102	82-83	0.6739371046543994
1102	84-85	0.6003702034603968
1102	86-87	0.5590933946666965
1102	88-89	0.5426368452539947
1102	90-91	0.5540532485747853
1102	92-93	0.5152295398536069
1102	94-95	0.5926238944501705
1102	96-97	0.5889476366473012
1102	98-99	0.582311055027791
1102	100	0.494202377073222
1103	1	0.12261357038130072
1103	2	0.15525697255055348
1103	3	0.1859769055955347
1103	4	0.11493031311858459
1103	5	0.2006490165114556
1103	6	0.27105548913897337
1103	7	0.2125050976159173
1103	8	0.263636449071349
1103	9	0.3630960090660693
1103	10-11	0.3917285920636431
1103	12-13	0.26262427403190003
1103	14-15	0.3255273142244093
1103	16-17	0.27550206274570854
1103	18-19	0.24620574233444614
1103	20-21	0.23328053960623407
1103	22-23	0.18931708168575767
1103	24-25	0.33042433484877165
1103	26-27	0.3293691246542423
1103	28-29	0.325674722755096
1103	30-31	0.2572558130689089
1103	32-33	0.3474806473165586
1103	34-35	0.35581302592289177
1103	36-37	0.2858929544666111
1103	38-39	0.3119533963859311
1103	40-41	0.23517089989227458
1103	42-43	0.18003926617976873
1103	44-45	0.13921102658427031
1103	46-47	0.18650239820207304
1103	48-49	0.20663593391522284
1103	50-51	0.09974470475621189
1103	52-53	0.17354610927061032
1103	54-55	0.2636137120647888
1103	56-57	0.19202803409519476
1103	58-59	0.28583016826775776
1103	60-61	0.31005604471209125
1103	62-63	0.2701992170651124
1103	64-65	0.3624181850315793
1103	66-67	0.36394424088317123
1103	68-69	0.36599590904091883
1103	70-71	0.3557926389615673
1103	72-73	0.33468274150100896
1103	74-75	0.30318190186982363
1103	76-77	0.30986508090207465
1103	78-79	0.2954544847932219
1103	80-81	0.24242285740006864
1103	82-83	0.23225666437916814
1103	84-85	0.2339964677642712
1103	86-87	0.19267830479473247
1103	88-89	0.23489499336064057
1103	90-91	0.24349280944679563
1103	92-93	0.22685019666464257
1103	94-95	0.21060810849404987
1103	96-97	0.12474184359427198
1103	98-99	0.19361873743305935
1103	100	0.1816980415547853
1104	1	0.2465195100410611
1104	2	0.31586338765166033
1104	3	0.3333617400751834
1104	4	0.39393290214935917
1104	5	0.21779878585422807
1104	6	0.32462597383899094
1104	7	0.25795928592992823
1104	8	0.3409047954202009
1104	9	0.45244776884032945
1104	10-11	0.45284367052106944
1104	12-13	0.41105424059878004
1104	14-15	0.3955589219552138
1104	16-17	0.44830782305742645
1104	18-19	0.4147630779136904
1104	20-21	0.3522103211743186
1104	22-23	0.35043583472244677
1104	24-25	0.4087330315699518
1104	26-27	0.5557727107578856
1104	28-29	0.51633802558122
1104	30-31	0.5121591144918654
1104	32-33	0.544150954700271
1104	34-35	0.5586154957798328
1104	36-37	0.4931090860759113
1104	38-39	0.5594766664725839
1104	40-41	0.5423516983981855
1104	42-43	0.48118345714614463
1104	44-45	0.4409907689700745
1104	46-47	0.4206507751729518
1104	48-49	0.45462979617738597
1104	50-51	0.43239456370157825
1104	52-53	0.4189006434712681
1104	54-55	0.41886288028447893
1104	56-57	0.4965629172808761
1104	58-59	0.5068345633185203
1104	60-61	0.4759860759610959
1104	62-63	0.5362209183524556
1104	64-65	0.5282751810416073
1104	66-67	0.5116295495978278
1104	68-69	0.5106599675764087
1104	70-71	0.42565522001461176
1104	72-73	0.4371806855620086
1104	74-75	0.4997922493262905
1104	76-77	0.40507057242188793
1104	78-79	0.33199462397644197
1104	80-81	0.4037035141450005
1104	82-83	0.3731094381944473
1104	84-85	0.3420593359265567
1104	86-87	0.33377853731703055
1104	88-89	0.3123538345015149
1104	90-91	0.2895087798190552
1104	92-93	0.2967664678444635
1104	94-95	0.37218416014196976
1104	96-97	0.4104857974828242
1104	98-99	0.41819138307139525
1104	100	0.44560330820305083
1105	1	0.167208204400481
1105	2	0.15873670072237545
1105	3	0.272907683395168
1105	4	0.19912444195369972
1105	5	0.09048860237596301
1105	6	0.15549596735730375
1105	7	0.2551336891392779
1105	8	0.1451101002276971
1105	9	0.3260389038799332
1105	10-11	0.34719254170334324
1105	12-13	0.3300308907385485
1105	14-15	0.4045863932171372
1105	16-17	0.37154871666983524
1105	18-19	0.3402382006616236
1105	20-21	0.3049672533343184
1105	22-23	0.30036377952802695
1105	24-25	0.3559206960742074
1105	26-27	0.4097862544219879
1105	28-29	0.5577749053604464
1105	30-31	0.5455271763412313
1105	32-33	0.5404947459799061
1105	34-35	0.5853613294436499
1105	36-37	0.4930490464299311
1105	38-39	0.4130760851547137
1105	40-41	0.4573226831725705
1105	42-43	0.4868941189300955
1105	44-45	0.4294230225726565
1105	46-47	0.45144378003608665
1105	48-49	0.36598019219904643
1105	50-51	0.3906205704532937
1105	52-53	0.37476133198733663
1105	54-55	0.5032460121611848
1105	56-57	0.49228991167715463
1105	58-59	0.40989788815410577
1105	60-61	0.4379731893806351
1105	62-63	0.4748615430512615
1105	64-65	0.5052241666192145
1105	66-67	0.5082810440630965
1105	68-69	0.4379847952424356
1105	70-71	0.39145946059652204
1105	72-73	0.4316700670401268
1105	74-75	0.4768459042740254
1105	76-77	0.39477750656670096
1105	78-79	0.47222228395181176
1105	80-81	0.4710446058501496
1105	82-83	0.45098977354177805
1105	84-85	0.43377905729688493
1105	86-87	0.4620819654969992
1105	88-89	0.4595087019681863
1105	90-91	0.45961624011841096
1105	92-93	0.4364466470125201
1105	94-95	0.4779469108903527
1105	96-97	0.597881628125613
1105	98-99	0.5681349320895386
1105	100	0.5710726441348513
1106	1	0.2516127730627069
1106	2	0.21379358721619468
1106	3	0.2780022103346447
1106	4	0.31027394634402583
1106	5	0.189798314379793
1106	6	0.3229907386140596
1106	7	0.3473944394361155
1106	8	0.32321014005271564
1106	9	0.3409737293170494
1106	10-11	0.4174628698172853
1106	12-13	0.35154183433865427
1106	14-15	0.48237422027557386
1106	16-17	0.4596724740786229
1106	18-19	0.3779151852386846
1106	20-21	0.4338291138967776
1106	22-23	0.4384724814978256
1106	24-25	0.39619079796844403
1106	26-27	0.3387536546097465
1106	28-29	0.5210157185300943
1106	30-31	0.4805659696941902
1106	32-33	0.5040506403228875
1106	34-35	0.44550665120902266
1106	36-37	0.4701249319784395
1106	38-39	0.5282276822991108
1106	40-41	0.5115624803496033
1106	42-43	0.4917616129546758
1106	44-45	0.431458131279733
1106	46-47	0.45477066980417646
1106	48-49	0.41255805358447617
1106	50-51	0.44507209744740095
1106	52-53	0.4750094861343328
1106	54-55	0.5209886972989182
1106	56-57	0.5080808049736802
1106	58-59	0.5454089262342023
1106	60-61	0.5288321352574243
1106	62-63	0.5907015208492012
1106	64-65	0.6358208218351749
1106	66-67	0.6624083745603997
1106	68-69	0.5772781750705747
1106	70-71	0.559055457779273
1106	72-73	0.5794336375796085
1106	74-75	0.5301168009682833
1106	76-77	0.5876537592860522
1106	78-79	0.5844202353623338
1106	80-81	0.5467029735803379
1106	82-83	0.540190939268193
1106	84-85	0.5755631641500649
1106	86-87	0.5860999335516723
1106	88-89	0.6044174130754918
1106	90-91	0.5819016498418499
1106	92-93	0.6045650379871503
1106	94-95	0.6129756611233148
1106	96-97	0.6282360803992795
1106	98-99	0.6421222865557858
1106	100	0.5623261105267723
1107	1	0.1959640136382994
1107	2	0.24675253238561368
1107	3	0.2989950602817615
1107	4	0.2789483897286331
1107	5	0.25911235275821554
1107	6	0.24210616361690285
1107	7	0.25596337713896844
1107	8	0.2724668800670429
1107	9	0.41632155777907087
1107	10-11	0.365342869006831
1107	12-13	0.28092596069001985
1107	14-15	0.4804903769007396
1107	16-17	0.35035634586208175
1107	18-19	0.39938250862571323
1107	20-21	0.3706093985973169
1107	22-23	0.3956260926185493
1107	24-25	0.4560155644331445
1107	26-27	0.4466791527039433
1107	28-29	0.5166646633837786
1107	30-31	0.438510877007829
1107	32-33	0.48498024098625336
1107	34-35	0.46843985034596614
1107	36-37	0.4366303718220621
1107	38-39	0.45069028482843976
1107	40-41	0.47735747188321653
1107	42-43	0.5309185497746256
1107	44-45	0.5011246463812213
1107	46-47	0.431159078712831
1107	48-49	0.4280509385770088
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2212	98-99	-0.18451877194865673
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2213	100	0.1318029468316908
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2214	3	-0.3929023954580124
2214	4	-0.466725946167422
2214	5	-0.37517244010042106
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2214	14-15	-0.6837556840533807
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2214	80-81	-0.26150740056429456
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2214	84-85	-0.3320873688345394
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2214	88-89	-0.4108458215346893
2214	90-91	-0.4442431881971416
2214	92-93	-0.40749621260058433
2214	94-95	-0.44036865875165176
2214	96-97	-0.34718631393898747
2214	98-99	-0.29024043873192085
2214	100	-0.30996642214761394
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2215	2	-0.8124861680789159
2215	3	-0.8933237741247666
2215	4	-0.8958696995705395
2215	5	-0.7580118625329888
2215	6	-0.7583555256778141
2215	7	-0.6690924122217581
2215	8	-0.9519154577778863
2215	9	-1.1141085594387619
2215	10-11	-0.8590725728715327
2215	12-13	-0.9843669906584154
2215	14-15	-1.1085876107177128
2215	16-17	-0.9242277788145614
2215	18-19	-1.0222634738852463
2215	20-21	-0.8845046337530533
2215	22-23	-1.0283097201078704
2215	24-25	-1.1315360118946955
2215	26-27	-0.9537713363673959
2215	28-29	-1.02607428908577
2215	30-31	-1.0879986897683835
2215	32-33	-1.0113933972466569
2215	34-35	-0.9684846414408455
2215	36-37	-0.9418377441340127
2215	38-39	-1.0649114496096317
2215	40-41	-1.1280257223095802
2215	42-43	-1.1894304279795236
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2215	64-65	-1.0307220544546993
2215	66-67	-0.9743104327591965
2215	68-69	-1.0232018039276412
2215	70-71	-1.0532929358224123
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2215	80-81	-0.9973321153150358
2215	82-83	-1.0280647205078495
2215	84-85	-1.0201980552261851
2215	86-87	-0.9735372839800576
2215	88-89	-0.9491810809646068
2215	90-91	-0.9519478486364434
2215	92-93	-0.9563480601797849
2215	94-95	-1.0460673811351455
2215	96-97	-1.015825767359221
2215	98-99	-1.0313977269700771
2215	100	-1.0750939507677693
2216	1	-0.7651443477027478
2216	2	-0.8162488939912897
2216	3	-0.8962207662375583
2216	4	-0.7436017768581848
2216	5	-0.9931344956719563
2216	6	-0.9589651415945433
2216	7	-1.0062103614418056
2216	8	-1.0724696020463078
2216	9	-1.2534243208241165
2216	10-11	-1.4034187410639163
2216	12-13	-1.47036988972377
2216	14-15	-1.747631233246885
2216	16-17	-1.50956894067307
2216	18-19	-1.4207714630823105
2216	20-21	-1.3114653999132102
2216	22-23	-1.112877588820055
2216	24-25	-1.2387561296760623
2216	26-27	-1.3446945012876128
2216	28-29	-4.753905008091866
2216	30-31	-2.3279306858938114
2216	32-33	-1.8484271469482039
2216	34-35	-1.5662502858033847
2216	36-37	-1.6633980998597409
2216	38-39	-1.6527657947570091
2216	40-41	-1.6635090942782043
2216	42-43	-1.9064513178826559
2216	44-45	-1.7005500416660126
2216	46-47	-1.6325051153229566
2216	48-49	-1.6531934904358891
2216	50-51	-1.6051473359641548
2216	52-53	-1.6267898992018353
2216	54-55	-1.8768531041471377
2216	56-57	-1.7801196035579103
2216	58-59	-1.8386707464070788
2216	60-61	-1.7822572499274187
2216	62-63	-1.8816745808051145
2216	64-65	-1.752036277546729
2216	66-67	-1.868744541375122
2216	68-69	-1.8738587843411345
2216	70-71	-1.9853536038430946
2216	72-73	-1.9933891712307847
2216	74-75	-1.915055902993256
2216	76-77	-1.751409218317722
2216	78-79	-1.7428353226192463
2216	80-81	-1.7348784584164356
2216	82-83	-1.812356101739919
2216	84-85	-1.7247744299297452
2216	86-87	-1.7865519742692726
2216	88-89	-1.71177855603122
2216	90-91	-1.9175060472581045
2216	92-93	-1.828765977246448
2216	94-95	-1.7451341037205879
2216	96-97	-1.91678815049222
2216	98-99	-2.1264098475283397
2216	100	-2.111965876199285
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	149349.0
3	27759.0
4	19326.0
5	17026.0
6	11596.0
7	10269.0
8	10796.0
9	11385.0
10	11871.0
11	12592.0
12	14099.0
13	15365.0
14	16107.0
15	16475.0
16	16129.0
17	15742.0
18	15267.0
19	14318.0
20	14127.0
21	14917.0
22	18511.0
23	28822.0
24	43170.0
25	52530.0
26	55075.0
27	44002.0
28	45019.0
29	53923.0
30	61823.0
31	70116.0
32	87534.0
33	112796.0
34	153332.0
35	228419.0
36	355332.0
37	627130.0
38	759931.0
39	289229.0
40	973.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.789244427078955	20.63800944551338	11.895429216576671	21.67731691083099
2	17.56070857046805	22.786569098341015	37.09631256693815	22.556409764252784
3	17.277873876861673	28.334745478768415	29.249384126652412	25.137996517717504
4	12.711731339460972	20.59901921060395	36.45951553399244	30.229733915942642
5	11.681628260588706	38.38105284379131	31.3264968574264	18.610822038193593
6	27.116574625325157	39.97271839551909	18.608223609207485	14.302483369948268
7	25.248357322207333	34.9313895772126	21.34017407373019	18.48007902684988
8	23.88112166091223	35.80030210794112	21.555515173541703	18.763061057604943
9	23.650529322492766	20.80495065950587	21.532955418226525	34.01156459977484
10-11	21.871556434231213	29.891932547919954	27.350128493537845	20.88638252431099
12-13	23.562049938535463	27.97107113546063	26.864737839085993	21.602141086917914
14-15	21.694282750805595	30.197199198959883	24.810479848923357	23.298038201311158
16-17	20.406476203023168	32.83205120269665	24.567788433964548	22.193684160315637
18-19	20.032194885388595	30.708139834661562	27.214644115766433	22.04502116418341
20-21	22.746252799016922	30.80578893414259	25.67598396988298	20.771974296957506
22-23	21.80954592909733	31.57742385468641	25.804639034316747	20.808391181899516
24-25	21.320089676625813	30.60434902106424	26.64715211976872	21.428409182541223
26-27	21.214732520967626	29.91433504534527	26.88849188964039	21.98244054404671
28-29	21.50347761405306	30.192337535642583	26.564507479635296	21.73967737066906
30-31	22.142048348692985	31.63925703535323	25.482524408020673	20.73617020793311
32-33	20.640558886599315	30.560597273852018	27.057150016219467	21.7416938233292
34-35	20.511182099554997	29.21201596927666	27.472925284489296	22.803876646679043
36-37	22.364865354073494	30.880621994659506	25.771860806462993	20.982651844804007
38-39	21.90488453554564	30.484955535118896	26.502025983583728	21.108133945751735
40-41	20.871346815839654	30.14323412065204	27.060167018929036	21.925252044579267
42-43	20.730838045247722	30.29618159963419	26.839690898226763	22.133289456891326
44-45	21.736090565849164	30.489839110014273	26.576645767758063	21.197424556378504
46-47	21.02434600596143	31.86446010106832	25.88379414498801	21.22739974798224
48-49	20.763983934926543	30.340166899935138	27.223147425058215	21.672701740080104
50-51	20.897932367171705	29.526579287556352	27.40312664765689	22.172361697615052
52-53	20.9077527244975	29.990532560170912	26.83102827412788	22.270686441203708
54-55	21.45125417035831	31.227251219786773	26.32026506308366	21.001229546771256
56-57	20.39162993405876	30.66408296245821	26.46584754725552	22.47843955622751
58-59	20.109034738905624	29.555801905602312	27.54083266221164	22.794330693280422
60-61	21.67257159916986	30.697315814586112	26.072800386024582	21.557312200219446
62-63	21.27108760846805	30.473593242595783	26.290589675793658	21.964729473142512
64-65	20.984475377468257	29.679149851022906	27.306476003245866	22.029898768262967
66-67	20.94534030462243	29.91744899654038	26.29816599069012	22.839044708147068
68-69	22.127466867685815	30.43698241295381	26.153116684092588	21.282434035267787
70-71	22.383803096331594	29.634442753015932	26.603003146075967	21.37875100457651
72-73	21.36275110633932	29.83997988693356	27.702895597313926	21.094373409413194
74-75	21.180364844017618	29.729928092217083	27.315360906714087	21.77434615705121
76-77	21.478450554586118	30.13714031968555	26.39153331292876	21.99287581279957
78-79	21.93213393432469	30.548261469570658	26.459486390032904	21.060118206071742
80-81	21.282200270610254	29.78795382618508	27.01149433446161	21.918351568743052
82-83	20.929271190747606	29.892663924942823	27.166631413227567	22.011433471082007
84-85	21.421783643372404	29.898415227579818	26.808255228511086	21.8715459005367
86-87	21.929765037369837	30.046532004274734	26.522646200042477	21.501056758312956
88-89	21.05944119032007	29.885198126180846	26.954740075662	22.100620607837087
90-91	20.835633693426935	29.540328784570878	26.884692300190217	22.73934522181197
92-93	21.448937782830964	30.26427060860588	26.286959406636484	21.99983220192667
94-95	21.320837589782933	29.792402613663533	26.763689111003476	22.12307068555007
96-97	21.110941706429447	29.755705199512338	27.023671417758194	22.10968167630002
98-99	21.008033668411404	29.7622493533359	26.99381294957901	22.235904028673684
100	21.63543412205422	30.280624427539	26.37448844061766	21.70945300978912
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	29158.0
1	22860.5
2	14388.5
3	10184.0
4	6888.5
5	4890.5
6	3908.5
7	3599.5
8	3586.5
9	3547.0
10	3238.5
11	2899.5
12	2724.5
13	2676.0
14	2700.0
15	2787.0
16	2973.5
17	3416.0
18	4005.0
19	4739.0
20	5778.5
21	7356.0
22	9942.5
23	14108.5
24	18813.0
25	23638.0
26	30456.0
27	38824.0
28	48854.5
29	58062.0
30	64288.0
31	70349.5
32	76870.0
33	83210.5
34	88853.0
35	93786.0
36	96849.5
37	98655.5
38	99428.0
39	99026.5
40	99129.5
41	98559.0
42	96340.5
43	94405.0
44	94068.0
45	94870.5
46	97516.5
47	102287.5
48	107124.5
49	111490.0
50	114396.5
51	111141.5
52	104251.0
53	99461.0
54	96889.5
55	94827.0
56	91933.5
57	88201.0
58	83665.0
59	77838.5
60	68939.5
61	58066.5
62	47209.5
63	36570.5
64	27341.0
65	20150.5
66	14944.0
67	10958.0
68	7907.5
69	5767.5
70	4233.5
71	3165.5
72	2410.5
73	1835.0
74	1462.5
75	1166.0
76	812.5
77	618.5
78	509.0
79	385.0
80	280.0
81	211.0
82	186.5
83	153.0
84	122.0
85	110.5
86	88.0
87	61.5
88	51.0
89	34.5
90	34.0
91	36.0
92	24.0
93	18.5
94	21.0
95	22.5
96	20.0
97	18.5
98	21.0
99	23.5
100	34.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014479660619468274
2	0.007779268646537856
3	0.010050587959395625
4	0.009227234708484683
5	0.010987507175949455
6	0.011214639107235231
7	0.009681498571056237
8	0.007438570749609192
9	0.006444868550233917
10-11	0.006104170653305253
12-13	0.006601021752992889
14-15	0.007410179258198469
16-17	0.005110468453929979
18-19	0.005507949333680088
20-21	0.005295013148099673
22-23	0.005649906790733699
24-25	0.005507949333680088
26-27	0.005820255739198031
28-29	0.016651609712388514
30-31	0.005224034419572867
32-33	0.005096272708224618
34-35	0.004798162048412036
36-37	0.006317106838885668
38-39	0.005365991876626478
40-41	0.005280817402394311
42-43	0.004840749285528119
44-45	0.0042019407287868715
46-47	0.005905430213430198
48-49	0.0037334811205099565
50-51	0.003832851340447484
52-53	0.0031372598008847924
54-55	0.004329702440135121
56-57	0.0030378895809472653
58-59	0.0026546044469025164
60-61	0.0035915236634563464
62-63	0.0033927832235812914
64-65	0.004656204591358425
66-67	0.003321804495054486
68-69	0.0036766981376885124
70-71	0.0049827067425817296
72-73	0.0026546044469025164
74-75	0.0028817363781882934
76-77	0.002725583175429322
78-79	0.0031940427837062364
80-81	0.0040457875260278995
82-83	0.001902229924518381
84-85	0.002810757649661488
86-87	0.0025552342269649893
88-89	0.0020016001444559083
90-91	0.0025126469898489063
92-93	0.0025978214640810723
94-95	0.0021577533472148798
96-97	0.0012492256220717724
98-99	0.001916425670223742
100	0.0030662810723579874
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3522182.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.47375014065632
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.24703503500808	56.44536211691301
2	12.68396818213619	17.62405672555529
3	3.122096971565595	6.5071135475234385
4	1.1295928912355495	3.1390821714544055
5	0.5337107012353374	1.8539441952509153
6	0.30832828599939155	1.285243338169113
7	0.19597153120101293	0.9530414035338703
8	0.1427842041498925	0.7935803298513675
9	0.10432654283069369	0.6523160552692283
>10	0.4875340589741108	5.980822267633689
>50	0.030454452999829195	1.42591920895692
>100	0.013338079037418816	1.6013122082971134
>500	5.727090878255902E-4	0.288862679681106
>1k	2.454467477887723E-4	0.4166397906349083
>5k	0.0	0.0
>10k+	4.0907791298128686E-5	1.0327039612756248
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36337	1.031661623391409	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4990	0.14167354213950328	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3732	0.10595704594481489	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	8.517447423216631E-5	0.0
6	0.0	0.0	0.0	8.517447423216631E-5	0.0
7	0.0	0.0	0.0	8.517447423216631E-5	0.0
8	2.8391491410722105E-5	0.0	0.0	8.517447423216631E-5	0.0
9	2.8391491410722105E-5	0.0	0.0	8.517447423216631E-5	0.0
10-11	2.8391491410722105E-5	0.0	0.0	1.1356596564288842E-4	0.0
12-13	2.8391491410722105E-5	0.0	0.0	1.4195745705361052E-4	0.0
14-15	2.8391491410722105E-5	0.0	0.0	1.9874043987505471E-4	0.0
16-17	2.8391491410722105E-5	0.0	0.0	2.2713193128577684E-4	0.0
18-19	2.8391491410722105E-5	0.0	0.0	2.555234226964989E-4	0.0
20-21	2.8391491410722105E-5	0.0	0.0	3.832851340447484E-4	0.0
22-23	2.8391491410722105E-5	0.0	0.0	7.807660137948578E-4	0.0
24-25	2.8391491410722105E-5	0.0	0.0	0.0017460767217594092	0.0
26-27	2.8391491410722105E-5	0.0	0.0	0.0031372598008847924	0.0
28-29	2.8391491410722105E-5	0.0	0.0	0.006160953636126697	0.0
30-31	2.8391491410722105E-5	0.0	0.0	0.012194145560905143	0.0
32-33	2.8391491410722105E-5	0.0	0.0	0.025126469898489063	0.0
34-35	2.8391491410722105E-5	0.0	0.0	0.042714998827431405	0.0
36-37	2.8391491410722105E-5	1.4195745705361052E-5	0.0	0.0671032899492417	0.0
38-39	2.8391491410722105E-5	2.8391491410722105E-5	0.0	0.10651068002732397	0.0
40-41	2.8391491410722105E-5	2.8391491410722105E-5	0.0	0.1686028717425732	0.0
42-43	2.8391491410722105E-5	2.8391491410722105E-5	0.0	0.2338465190044126	0.0
44-45	2.8391491410722105E-5	2.8391491410722105E-5	0.0	0.29874946836932337	0.0
46-47	2.8391491410722105E-5	2.8391491410722105E-5	0.0	0.37475349087582643	0.0
48-49	2.8391491410722105E-5	2.8391491410722105E-5	0.0	0.4665573783523963	0.0
50-51	2.8391491410722105E-5	2.8391491410722105E-5	0.0	0.5741327393076224	0.0
52-53	2.8391491410722105E-5	2.8391491410722105E-5	0.0	0.6826592152251076	0.0
54-55	2.8391491410722105E-5	2.8391491410722105E-5	0.0	0.8067726199270793	0.0
56-57	2.8391491410722105E-5	2.8391491410722105E-5	0.0	0.9447978554203048	0.0
58-59	2.8391491410722105E-5	2.8391491410722105E-5	0.0	1.092092912859131	0.0
60-61	2.8391491410722105E-5	2.8391491410722105E-5	0.0	1.2499354093570405	0.0
62-63	2.8391491410722105E-5	2.8391491410722105E-5	0.0	1.4126612423775944	0.0
64-65	2.8391491410722105E-5	2.8391491410722105E-5	0.0	1.6053117073450491	0.0
66-67	2.8391491410722105E-5	2.8391491410722105E-5	0.0	1.8356092899231213	0.0
68-69	2.8391491410722105E-5	2.8391491410722105E-5	0.0	2.066701834260694	0.0
70-71	2.8391491410722105E-5	2.8391491410722105E-5	0.0	2.322026516517318	0.0
72-73	2.8391491410722105E-5	2.8391491410722105E-5	0.0	2.64116107571954	0.0
74-75	2.8391491410722105E-5	2.8391491410722105E-5	0.0	2.9515226640758483	1.4195745705361052E-5
76-77	2.8391491410722105E-5	2.8391491410722105E-5	0.0	3.264482073896238	2.8391491410722105E-5
78-79	2.8391491410722105E-5	2.8391491410722105E-5	0.0	3.6045553580138674	2.8391491410722105E-5
80-81	2.8391491410722105E-5	2.8391491410722105E-5	0.0	3.9801180063948998	2.8391491410722105E-5
82-83	2.8391491410722105E-5	2.8391491410722105E-5	0.0	4.389736248723093	2.8391491410722105E-5
84-85	2.8391491410722105E-5	2.8391491410722105E-5	0.0	4.795649401422187	2.8391491410722105E-5
86-87	2.8391491410722105E-5	2.8391491410722105E-5	0.0	5.228605449689994	2.8391491410722105E-5
88	2.8391491410722105E-5	2.8391491410722105E-5	0.0	5.5775652706191785	2.8391491410722105E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGC	360	0.0	52.229218	3
TACCTGG	4505	0.0	51.024208	2
GTACCTG	4895	0.0	47.63319	1
TATAACG	410	0.0	47.006966	2
GGTATCA	5690	0.0	36.929718	1
TCTGTCG	1500	0.0	36.033554	8
GTCTCGC	1350	0.0	35.517887	1
ACCTGGG	7110	0.0	31.601982	3
GTATCAA	8760	0.0	29.890404	1
TCGCTCT	1565	0.0	29.735191	4
CTGTCGC	1875	0.0	29.57885	9
GTACACG	355	0.0	29.132318	1
TAACGCA	700	0.0	28.874887	4
TCTCGCT	1685	0.0	28.455256	2
GTATAAC	1065	0.0	27.808119	1
CTCGCTC	1705	0.0	27.018282	3
GTACATG	14490	0.0	26.08365	1
TCAACGC	9650	0.0	25.816565	4
CGCTCTG	1605	0.0	25.479689	5
ATCAACG	9935	0.0	25.076334	3
>>END_MODULE