##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431441_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3522182
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24097136377393	33.0	31.0	34.0	30.0	34.0
2	32.33881099840951	34.0	31.0	34.0	30.0	34.0
3	32.45131057963501	34.0	31.0	34.0	30.0	34.0
4	36.006902539391774	37.0	35.0	37.0	35.0	37.0
5	35.93711852482353	37.0	35.0	37.0	35.0	37.0
6	35.98084795163907	37.0	35.0	37.0	35.0	37.0
7	35.91962198432676	37.0	35.0	37.0	35.0	37.0
8	35.90045829545435	37.0	35.0	37.0	35.0	37.0
9	37.583452246363194	39.0	37.0	39.0	35.0	39.0
10-11	37.51184237498233	39.0	37.0	39.0	34.5	39.0
12-13	37.49594668872875	39.0	37.0	39.0	34.0	39.0
14-15	38.79699288679574	40.0	38.0	41.0	34.0	41.0
16-17	38.8554384753542	40.0	38.0	41.0	35.0	41.0
18-19	38.95032340747866	40.0	38.5	41.0	35.0	41.0
20-21	38.934661099284476	40.0	38.5	41.0	35.0	41.0
22-23	38.80532649931207	40.0	38.0	41.0	34.5	41.0
24-25	38.71827094681649	40.0	38.0	41.0	34.0	41.0
26-27	38.54966026741378	40.0	38.0	41.0	34.0	41.0
28-29	38.41750497276972	40.0	38.0	41.0	34.0	41.0
30-31	38.162560594540544	40.0	37.5	41.0	34.0	41.0
32-33	37.849583581995475	40.0	37.0	41.0	33.0	41.0
34-35	37.62278070809515	40.0	36.5	41.0	33.0	41.0
36-37	37.41092325723089	40.0	36.0	41.0	33.0	41.0
38-39	37.25978654708928	40.0	36.0	41.0	32.5	41.0
40-41	37.11220189643806	40.0	36.0	41.0	32.0	41.0
42-43	36.95445238207452	40.0	36.0	41.0	31.0	41.0
44-45	36.680587204181954	40.0	36.0	41.0	30.5	41.0
46-47	36.28417427038126	40.0	35.0	41.0	29.5	41.0
48-49	35.95136225214938	40.0	35.0	41.0	28.0	41.0
50-51	35.78786857124362	40.0	35.0	41.0	27.0	41.0
52-53	35.63305062032569	40.0	35.0	41.0	27.0	41.0
54-55	35.45439801236847	39.0	35.0	41.0	26.5	41.0
56-57	35.239370935403116	39.0	35.0	41.0	26.0	41.0
58-59	35.07521772015188	39.0	35.0	41.0	26.0	41.0
60-61	34.834510539205525	39.0	35.0	41.0	26.0	41.0
62-63	34.5426420326945	38.0	35.0	41.0	25.0	41.0
64-65	34.2558757611049	37.5	34.0	40.0	24.0	41.0
66-67	33.98120142570713	37.0	34.0	40.0	23.5	41.0
68-69	33.65713370291484	36.5	34.0	39.5	23.0	41.0
70-71	33.290707720384695	36.0	34.0	39.0	22.0	41.0
72-73	32.95214074684386	35.5	33.5	39.0	22.0	41.0
74-75	32.66147845284542	35.0	33.0	38.5	21.0	40.5
76-77	32.32917819124622	35.0	33.0	37.0	20.0	39.5
78-79	32.02719635157979	35.0	33.0	37.0	19.5	39.0
80-81	31.745602725810308	35.0	33.0	36.0	19.0	39.0
82-83	31.510738655753734	35.0	33.0	36.0	18.0	38.0
84-85	31.289885644750896	35.0	33.0	36.0	17.5	37.0
86-87	31.05355274088619	35.0	33.0	35.0	14.0	37.0
88-89	30.91907516420219	35.0	33.0	35.0	11.0	36.5
90-91	30.744002439396944	35.0	33.0	35.0	4.5	36.0
92-93	30.54304419817034	35.0	33.0	35.0	2.0	36.0
94-95	30.368143241888127	35.0	32.5	35.0	2.0	36.0
96-97	30.19277013510375	35.0	32.0	35.0	2.0	35.5
98-99	30.028261032507693	35.0	32.0	35.0	2.0	35.0
100	29.916073899645163	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1756851377608868
1101	2	0.24148620571759238
1101	3	0.2372004425325045
1101	4	0.20633813503057752
1101	5	0.16326712874189298
1101	6	0.2480236797064279
1101	7	0.2502532319224784
1101	8	0.31062279381636415
1101	9	0.3926502023415992
1101	10-11	0.37321817603875473
1101	12-13	0.3760066423355255
1101	14-15	0.4782657368084102
1101	16-17	0.491637836680475
1101	18-19	0.4523608530659118
1101	20-21	0.3959609659240968
1101	22-23	0.4135910653284327
1101	24-25	0.4384877801397451
1101	26-27	0.4441982837049494
1101	28-29	0.5022299038256577
1101	30-31	0.5116289657360724
1101	32-33	0.4667617805240809
1101	34-35	0.4863960459976795
1101	36-37	0.41607614873473153
1101	38-39	0.4015596825635086
1101	40-41	0.44198358185031594
1101	42-43	0.3631169191944821
1101	44-45	0.36945662648181354
1101	46-47	0.36439877313349456
1101	48-49	0.2820875986252318
1101	50-51	0.29023585640072014
1101	52-53	0.2982461983216993
1101	54-55	0.3048604431338262
1101	56-57	0.287060872731395
1101	58-59	0.3304976413649072
1101	60-61	0.36330747101296623
1101	62-63	0.31024073242159744
1101	64-65	0.30698741581175426
1101	66-67	0.34104657909647784
1101	68-69	0.30501532485962457
1101	70-71	0.2874280010310315
1101	72-73	0.3240239538380436
1101	74-75	0.2542512345866399
1101	76-77	0.31042686047823054
1101	78-79	0.3222054500382683
1101	80-81	0.3519517640579899
1101	82-83	0.28725797437868295
1101	84-85	0.2513122079433643
1101	86-87	0.28800599977583374
1101	88-89	0.2395170326333016
1101	90-91	0.3182015208045499
1101	92-93	0.322147344152377
1101	94-95	0.3167520413970486
1101	96-97	0.33110527990438143
1101	98-99	0.4200933285683419
1101	100	0.473793059664704
1102	1	0.19151062897874027
1102	2	0.2662870875085588
1102	3	0.17158479815072525
1102	4	0.19816100539811288
1102	5	0.1990926965927926
1102	6	0.09585445349588184
1102	7	0.15793876053726308
1102	8	0.1590861734360587
1102	9	0.2154674376254988
1102	10-11	0.2616861153249488
1102	12-13	0.2626279805529421
1102	14-15	0.44796866878891706
1102	16-17	0.37538460532018547
1102	18-19	0.29523053090056095
1102	20-21	0.3136503006470335
1102	22-23	0.30350091931692447
1102	24-25	0.26439236943769373
1102	26-27	0.2798998337361098
1102	28-29	0.32126964061667707
1102	30-31	0.31070707014093557
1102	32-33	0.37208592555468556
1102	34-35	0.37998684083567014
1102	36-37	0.3661572520463068
1102	38-39	0.37649345883587415
1102	40-41	0.4200785991693792
1102	42-43	0.4597573262692478
1102	44-45	0.4694660140304805
1102	46-47	0.5000291182887437
1102	48-49	0.508314749204068
1102	50-51	0.4706663297886209
1102	52-53	0.4711417908995301
1102	54-55	0.4468166550088881
1102	56-57	0.5086743943776781
1102	58-59	0.4021254678472488
1102	60-61	0.4280522446389412
1102	62-63	0.47699399443510515
1102	64-65	0.46690303005119205
1102	66-67	0.415716993119311
1102	68-69	0.4140499144641012
1102	70-71	0.39834725278592487
1102	72-73	0.3610791628419108
1102	74-75	0.3671238627937399
1102	76-77	0.36705664110334624
1102	78-79	0.3935577807047679
1102	80-81	0.34363356575209636
1102	82-83	0.33636571022966777
1102	84-85	0.3089361746744004
1102	86-87	0.3277052405977514
1102	88-89	0.3230586162808322
1102	90-91	0.36487768996945036
1102	92-93	0.3629578280645447
1102	94-95	0.3986165745995933
1102	96-97	0.33932379138401103
1102	98-99	0.3979349459344128
1102	100	0.35018880295420374
1103	1	0.08051968954526245
1103	2	0.10070013168282088
1103	3	0.08981575321874402
1103	4	0.05737554952835211
1103	5	0.08082859148381516
1103	6	0.05307689360569867
1103	7	0.09654281698171019
1103	8	0.12846824199861828
1103	9	0.19006663562128523
1103	10-11	0.19365974670182595
1103	12-13	0.17474994002051147
1103	14-15	0.19275245648458394
1103	16-17	0.2287805502097484
1103	18-19	0.1896228046065147
1103	20-21	0.20602989329506727
1103	22-23	0.11880166422569971
1103	24-25	0.1507621831571342
1103	26-27	0.11928955282120057
1103	28-29	0.1759417286823961
1103	30-31	0.16228626605568053
1103	32-33	0.12795013111428943
1103	34-35	0.17571171950785214
1103	36-37	0.19041584504383735
1103	38-39	0.1839183176313739
1103	40-41	0.1746619925329682
1103	42-43	0.18377222978061525
1103	44-45	0.20245902584176179
1103	46-47	0.24537418887098994
1103	48-49	0.22514002914515885
1103	50-51	0.3028924384140268
1103	52-53	0.29012013932047154
1103	54-55	0.21809021021403652
1103	56-57	0.2628007240478354
1103	58-59	0.27131045374681406
1103	60-61	0.28747981279995116
1103	62-63	0.24080128664370903
1103	64-65	0.2649773428381721
1103	66-67	0.29855285906240425
1103	68-69	0.2190053620096606
1103	70-71	0.2574066353814288
1103	72-73	0.22814658148758582
1103	74-75	0.19535670881479206
1103	76-77	0.1962892410003647
1103	78-79	0.26739261012546933
1103	80-81	0.2434808387776961
1103	82-83	0.16604086650843186
1103	84-85	0.22730803409154632
1103	86-87	0.25543915182234045
1103	88-89	0.2356089583198866
1103	90-91	0.21344303356929473
1103	92-93	0.2475697558094616
1103	94-95	0.29698401845796596
1103	96-97	0.2862632155204423
1103	98-99	0.33139382587657096
1103	100	0.35468473557090974
1104	1	0.11292261358973121
1104	2	0.1625673877848044
1104	3	0.1066322310797645
1104	4	0.11186888536537509
1104	5	0.0422659853039562
1104	6	0.07696082547217031
1104	7	0.06849668998031433
1104	8	0.07899213541455197
1104	9	0.1675342425781352
1104	10-11	0.170623587095875
1104	12-13	0.2200331770661137
1104	14-15	0.2652749607032021
1104	16-17	0.1953340813693032
1104	18-19	0.2119711115460987
1104	20-21	0.16885565109569
1104	22-23	0.1586775951918611
1104	24-25	0.13698842358954266
1104	26-27	0.15231507202020111
1104	28-29	0.12051327988341143
1104	30-31	0.07739251298328043
1104	32-33	0.16306656924540164
1104	34-35	0.22550115738611964
1104	36-37	0.23332929592455542
1104	38-39	0.2696975808139399
1104	40-41	0.2512022285409543
1104	42-43	0.2357622485757318
1104	44-45	0.22479314636574088
1104	46-47	0.2960962992508982
1104	48-49	0.20461577779504836
1104	50-51	0.2100133206432062
1104	52-53	0.08468919450598378
1104	54-55	0.0895990622992926
1104	56-57	0.08310927442075666
1104	58-59	0.2076442014160662
1104	60-61	0.17961745920575112
1104	62-63	0.1723464940479218
1104	64-65	0.16827955944278727
1104	66-67	0.07411402207706175
1104	68-69	0.14091579084654882
1104	70-71	0.16141583151205197
1104	72-73	0.16688840872330246
1104	74-75	0.10258636017738354
1104	76-77	0.19704979160630387
1104	78-79	0.1853308227497905
1104	80-81	0.16169041868360523
1104	82-83	0.18161802703802365
1104	84-85	0.2151858709183223
1104	86-87	0.20498721825556032
1104	88-89	0.1709837638171834
1104	90-91	0.15284023352402443
1104	92-93	0.15826934490737443
1104	94-95	0.20418287637015808
1104	96-97	0.2559137900167059
1104	98-99	0.17785809888685833
1104	100	0.21422814965345438
1105	1	0.08805690467732319
1105	2	0.1425672140660339
1105	3	0.1600218767247057
1105	4	0.11006327682432016
1105	5	0.0633995216291936
1105	6	0.0994419775567863
1105	7	0.12808066704455712
1105	8	0.16318349090936835
1105	9	0.21547704277693214
1105	10-11	0.15108675867799093
1105	12-13	0.09497162309530438
1105	14-15	0.1810870148202568
1105	16-17	0.2087791714939229
1105	18-19	0.17304700143003515
1105	20-21	0.15016413974922926
1105	22-23	0.18146963149663975
1105	24-25	0.14134017408427724
1105	26-27	0.16626945148951222
1105	28-29	0.1666804913641542
1105	30-31	0.1157921333620493
1105	32-33	0.1667384736092572
1105	34-35	0.12855783443095703
1105	36-37	0.16914142851982206
1105	38-39	0.17092941072558432
1105	40-41	0.15490137932282977
1105	42-43	0.25646333563619095
1105	44-45	0.16778561679983994
1105	46-47	0.17846533720167912
1105	48-49	0.10837209195813102
1105	50-51	0.06494953575764129
1105	52-53	0.10701158058513727
1105	54-55	0.13013841413474125
1105	56-57	0.17712341695747114
1105	58-59	0.1905918181202324
1105	60-61	0.2576447316815873
1105	62-63	0.14827737713891764
1105	64-65	0.16601001510941416
1105	66-67	0.16452799543606744
1105	68-69	0.22027883115669766
1105	70-71	0.19999355172897992
1105	72-73	0.1884586229939842
1105	74-75	0.18833537495194008
1105	76-77	0.24634870302199374
1105	78-79	0.1799583030329046
1105	80-81	0.2046598713803256
1105	82-83	0.11780769319397066
1105	84-85	0.18305105996968152
1105	86-87	0.14589845398194967
1105	88-89	0.16045000603098458
1105	90-91	0.139013514494863
1105	92-93	0.18302632720200052
1105	94-95	0.2285804208095925
1105	96-97	0.18507586513620566
1105	98-99	0.18927155558490227
1105	100	0.27296962876670605
1106	1	0.07602682159628671
1106	2	0.13580313394079013
1106	3	0.16567722153861553
1106	4	0.13555254532367655
1106	5	0.08462806023584335
1106	6	0.06904170839969481
1106	7	0.10245459476499974
1106	8	0.11716293581795867
1106	9	0.1332518718128668
1106	10-11	0.10629102435366633
1106	12-13	0.14773840469785426
1106	14-15	0.18839496482393514
1106	16-17	0.18566533928994744
1106	18-19	0.06929157317358658
1106	20-21	0.06554815546395076
1106	22-23	0.1104743283648304
1106	24-25	0.11437025694352343
1106	26-27	0.16556554595412365
1106	28-29	0.16941579072416602
1106	30-31	0.09999390271065067
1106	32-33	0.1641090761217896
1106	34-35	0.223093959463327
1106	36-37	0.20944938155454906
1106	38-39	0.21930241050166188
1106	40-41	0.23417105635279967
1106	42-43	0.25053218390360854
1106	44-45	0.29671809709265773
1106	46-47	0.20370206762311938
1106	48-49	0.1998616067856105
1106	50-51	0.23560206798045868
1106	52-53	0.18777591561416074
1106	54-55	0.2260391393167538
1106	56-57	0.2789939767026439
1106	58-59	0.1971190883514211
1106	60-61	0.2693591421652286
1106	62-63	0.1714590567729246
1106	64-65	0.16975128984859822
1106	66-67	0.17189534659477346
1106	68-69	0.2110783795748432
1106	70-71	0.25037707914921015
1106	72-73	0.28652702828477317
1106	74-75	0.28479207572381426
1106	76-77	0.30503759865804625
1106	78-79	0.2722025304197899
1106	80-81	0.23002653027716136
1106	82-83	0.21769034726567327
1106	84-85	0.20107027282416823
1106	86-87	0.2263190422522534
1106	88-89	0.19797752665634505
1106	90-91	0.2200286318115907
1106	92-93	0.21879986885559077
1106	94-95	0.21995390932373127
1106	96-97	0.21268341135248647
1106	98-99	0.26373142091911816
1106	100	0.2584943767960226
1107	1	0.10631179060561635
1107	2	0.17559242756905746
1107	3	0.0952165285729194
1107	4	0.12475354486625889
1107	5	0.09797102157298099
1107	6	0.09358756953578506
1107	7	0.04883485409250454
1107	8	0.06836872373168035
1107	9	0.06962410470145386
1107	10-11	0.08977559352304354
1107	12-13	0.062327457969679756
1107	14-15	0.16927484323387176
1107	16-17	0.11918183594516307
1107	18-19	0.10398045183403326
1107	20-21	0.09481576922321011
1107	22-23	0.1436723448585795
1107	24-25	0.1507550438956642
1107	26-27	0.17108614797069777
1107	28-29	0.18581471970181695
1107	30-31	0.1467235494664365
1107	32-33	0.18750987564416732
1107	34-35	0.21701979018472173
1107	36-37	0.188514523004514
1107	38-39	0.17047224016946672
1107	40-41	0.14330609032322883
1107	42-43	0.11227480462361683
1107	44-45	0.149884327141379
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2216	76-77	-0.791851058840372
2216	78-79	-0.7113019875758795
2216	80-81	-0.8230093486392356
2216	82-83	-0.9207801477954796
2216	84-85	-0.9310318927732268
2216	86-87	-0.9426300696633376
2216	88-89	-0.8896688550239134
2216	90-91	-0.8877583016143618
2216	92-93	-0.8074085068548662
2216	94-95	-0.9067037768378192
2216	96-97	-1.0602536653627794
2216	98-99	-1.1770224713951407
2216	100	-1.1735678898798554
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	121.0
3	441.0
4	293.0
5	363.0
6	917.0
7	2131.0
8	3681.0
9	5101.0
10	7136.0
11	9993.0
12	14277.0
13	19920.0
14	18747.0
15	20884.0
16	26004.0
17	37124.0
18	40882.0
19	23874.0
20	15965.0
21	14452.0
22	14430.0
23	14968.0
24	16756.0
25	19765.0
26	23720.0
27	30054.0
28	36872.0
29	46124.0
30	57450.0
31	70960.0
32	88069.0
33	111735.0
34	150264.0
35	230391.0
36	334581.0
37	651918.0
38	942665.0
39	416242.0
40	2912.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.09785006131198	19.805545716412837	13.053870750306986	24.042733471968194
2	15.502009833676967	21.61614590046738	38.97882051523743	23.903023750618225
3	15.79446013233857	27.307166190919368	31.229511966743306	25.668861709998758
4	11.208301169983576	18.64206218945262	37.08999364451262	33.05964299605119
5	10.63321796360601	35.8477403528099	33.602090851530804	19.91695083205329
6	26.79938174688304	38.30514720704382	19.372366334278013	15.523104711795133
7	24.350360089285562	32.6814173713908	23.630067952195542	19.33815458712809
8	24.70803609807784	31.483750697720904	23.44716996452767	20.361043239673588
9	24.23594237890035	15.363771661998161	24.48090416679206	35.91938179230942
10-11	22.171127522621354	26.075740612070646	29.658599067574578	22.09453279773342
12-13	25.05221195270432	24.113745399868606	29.749016376780073	21.085026270647003
14-15	22.403541327506645	25.751295645710528	29.364396842639024	22.480766184143803
16-17	19.365722725287903	28.793742061029214	29.140856435016705	22.69967877866618
18-19	18.80279042934181	25.112998135814674	34.640629019170504	21.443582415673013
20-21	21.18169929890051	25.290544327351625	33.72303021252167	19.804726161226192
22-23	20.37606233862986	26.11447960383649	33.74656675890116	19.762891298632496
24-25	19.90865269683791	25.243472617102793	34.53127652845042	20.31659815760888
26-27	19.785817427946654	24.586875408482584	34.75281799747997	20.874489166090793
28-29	20.013852253887197	24.926859730385	34.35102020188972	20.708267813838084
30-31	20.948311071380353	26.287573921992095	33.31039208663273	19.453722919994814
32-33	19.24664447394011	25.28581061418016	34.80163535315536	20.66590955872437
34-35	19.172908048227157	24.218145672631866	34.7708704706124	21.838075808528576
36-37	21.223424492623593	25.991456655801752	32.798477586853075	19.986641264721584
38-39	20.814091337485454	25.554070818683723	33.36316845277531	20.26866939105551
40-41	19.778882091269303	25.46989791456804	33.752982554963616	20.998237439199034
42-43	19.73665475476111	25.604691397037538	33.35865908654381	21.299994761657548
44-45	20.78225940624306	25.88155580830292	32.745425988776276	20.59075879667774
46-47	20.245218320820914	27.52259794572518	31.446247232705428	20.785936500748473
48-49	20.272182294877734	26.158042440700118	32.248308318627636	21.321466945794505
50-51	20.2977068937419	25.51243256196123	32.20129796753207	21.988562576764803
52-53	20.46158659639153	26.150474311756778	31.43737812862349	21.950560963228206
54-55	20.950057010859318	27.495531120888007	30.834627055854742	20.719784812397933
56-57	19.78985289873271	26.723645332295654	31.17098241988165	22.31551934908999
58-59	19.433783220121022	25.62503389554748	32.24898341751734	22.692199466814163
60-61	21.044867884613556	26.82561785419872	30.69014072310516	21.43937353808257
62-63	20.729024069168247	26.50229118970517	31.025742328003197	21.742942413123387
64-65	20.3198541567029	25.842240923939208	32.05587006636455	21.782034852993345
66-67	20.31182703197483	26.29250318765922	30.943745870806605	22.451923909559344
68-69	21.524930915834716	26.59857479217967	30.63276921814583	21.243725073839776
70-71	21.86530648111042	25.857054173901204	30.884272506708584	21.393366838279793
72-73	20.881146970826606	26.121620063926283	31.887875186461116	21.10935777878599
74-75	20.65158941698475	26.01421793143549	31.482619247441633	21.85157340413813
76-77	20.834386184473146	26.48189389418264	30.53263573546171	22.151084185882503
78-79	21.33304165650482	26.9546014764223	30.515751258530628	21.196605608542253
80-81	20.740509718123594	26.15309203215507	30.99780193073498	22.108596318986358
82-83	20.307954210883306	26.282960364800218	31.179859897954287	22.229225526362185
84-85	20.837488570583716	26.355733546005773	30.743053661792626	22.06372422161789
86-87	21.141042683200357	26.56855892171387	30.37063388547213	21.919764509613643
88-89	20.13878044916475	26.548031873423916	30.77025832282375	22.54292935458758
90-91	19.86862403314537	26.25005270171341	30.614265619190835	23.267057645950388
92-93	20.530002886005345	26.874402268261083	30.055964086907235	22.53963075882634
94-95	20.35678451596198	26.40682679089269	30.59522477827665	22.64116391486868
96-97	19.96992188320696	26.457130591948925	30.79933574889514	22.773611775948975
98-99	20.039691609256266	26.33934338494473	30.65597624181786	22.964988763981143
100	20.41920239398211	26.867111563484393	29.979174740536006	22.734511301997493
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2587.0
1	1754.5
2	10399.0
3	17864.5
4	10242.0
5	4366.0
6	5891.0
7	12814.0
8	19778.0
9	20327.5
10	13842.0
11	7223.5
12	5882.0
13	6102.0
14	6460.0
15	6822.0
16	7236.0
17	7810.5
18	8449.0
19	9175.0
20	10157.5
21	11349.5
22	13506.5
23	17390.0
24	21664.5
25	26078.0
26	32232.5
27	40048.5
28	49572.5
29	58341.5
30	63647.0
31	69011.0
32	75639.5
33	81728.5
34	87323.5
35	91469.5
36	94489.0
37	96575.0
38	96982.5
39	96666.0
40	96446.5
41	95812.0
42	93259.0
43	91472.5
44	91494.5
45	92534.0
46	96048.5
47	101252.5
48	106424.5
49	110817.0
50	113971.5
51	111051.5
52	103436.5
53	97712.0
54	94440.0
55	92229.5
56	89230.5
57	85167.0
58	81164.5
59	75347.5
60	65634.5
61	55035.5
62	44529.5
63	34144.0
64	25171.0
65	17856.0
66	12811.0
67	9096.5
68	6275.5
69	4304.5
70	2921.5
71	2068.0
72	1556.0
73	1164.5
74	876.5
75	652.0
76	394.5
77	280.0
78	227.0
79	123.5
80	53.0
81	30.0
82	19.0
83	11.0
84	8.0
85	4.5
86	3.0
87	3.0
88	4.0
89	2.5
90	0.5
91	0.5
92	0.5
93	0.5
94	1.0
95	2.0
96	1.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.047215050216030854
2	0.0
3	1.135659656428884E-4
4	0.06782727298021511
5	0.004883336522644202
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.01132820507287812
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.678298282144421E-4
26-27	0.0
28-29	3.2650215122330417E-4
30-31	0.0040173960346171775
32-33	1.9874043987505474E-4
34-35	0.001660902247527243
36-37	0.0040315917803225385
38-39	0.0013202043505985777
40-41	9.653107079645515E-4
42-43	0.0021435576015095188
44-45	0.0
46-47	4.116766254554705E-4
48-49	5.820255739198031E-4
50-51	0.0017034894846433264
52-53	7.381787766787747E-4
54-55	0.0013060086048932167
56-57	0.006260323856064224
58-59	0.009454366639770461
60-61	0.0014905532990629104
62-63	0.002938519361009738
64-65	0.0012918128591878557
66-67	1.4195745705361052E-4
68-69	8.23353250910941E-4
70-71	4.400681168661926E-4
72-73	0.0
74-75	2.8391491410722104E-4
76-77	0.0
78-79	5.394383368037199E-4
80-81	0.0
82-83	9.937021993752736E-4
84-85	5.820255739198031E-4
86-87	0.0
88-89	0.0
90-91	1.419574570536105E-5
92-93	3.548936426340263E-4
94-95	0.0
96-97	5.678298282144421E-4
98-99	7.665702680894968E-4
100	4.8265535398227575E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3522182.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.294122876496594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.25202615703445	49.52893358918165
2	14.434892441600661	18.272877118153684
3	3.7817054894588815	7.180791957975965
4	1.342667281348409	3.3993179155167135
5	0.632621874876305	2.0020623341390253
6	0.37703453828520306	1.4318442236944082
7	0.2350096706535828	1.0412311680059005
8	0.17283006234350226	0.875130176217776
9	0.12403270874087455	0.7065487356974665
>10	0.5875682348560812	6.666604083639145
>50	0.038125156629117786	1.6609149119960396
>100	0.01942012879332759	2.173453950273731
>500	0.0010780462902951764	0.47505075376819567
>1k	6.737789245191457E-4	0.6453514259096154
>5k	8.983718993588596E-5	0.3439846205595149
>10k+	2.2459297483971486E-4	3.595903035271253
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32039	0.9096349933081255	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30537	0.8669909732092208	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27490	0.7804820988807506	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21875	0.6210638746095459	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14538	0.41275550212907797	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6066	0.17222278689744028	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6033	0.17128586768088644	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3869	0.10984668026808382	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.8391491410722105E-5	0.0
8	0.0	0.0	0.0	2.8391491410722105E-5	0.0
9	0.0	0.0	0.0	2.8391491410722105E-5	0.0
10-11	0.0	0.0	0.0	2.8391491410722105E-5	0.0
12-13	0.0	0.0	0.0	2.8391491410722105E-5	0.0
14-15	0.0	0.0	0.0	9.937021993752736E-5	0.0
16-17	0.0	0.0	0.0	1.4195745705361052E-4	0.0
18-19	0.0	0.0	0.0	1.4195745705361052E-4	0.0
20-21	0.0	0.0	0.0	2.1293618558041578E-4	0.0
22-23	0.0	0.0	0.0	6.246128110358863E-4	0.0
24-25	0.0	0.0	0.0	0.0016183150104111597	0.0
26-27	0.0	0.0	0.0	0.0030804768180633484	0.0
28-29	0.0	0.0	0.0	0.006146757890421336	0.0
30-31	0.0	0.0	0.0	0.012336103017958754	0.0
32-33	2.8391491410722105E-5	0.0	0.0	0.025566538015355255	0.0
34-35	5.678298282144421E-5	0.0	0.0	0.04349576484122626	0.0
36-37	5.678298282144421E-5	0.0	0.0	0.06839510280842956	0.0
38-39	5.678298282144421E-5	0.0	0.0	0.10859745464601206	0.0
40-41	5.678298282144421E-5	0.0	0.0	0.17205243794897596	0.0
42-43	5.678298282144421E-5	0.0	0.0	0.23907055342398548	0.0
44-45	5.678298282144421E-5	0.0	0.0	0.3061454518818164	0.0
46-47	5.678298282144421E-5	0.0	0.0	0.38413687878707004	0.0
48-49	5.678298282144421E-5	0.0	0.0	0.4782972600507299	0.0
50-51	5.678298282144421E-5	0.0	0.0	0.5881013530816976	0.0
52-53	5.678298282144421E-5	0.0	0.0	0.6992114547175585	0.0
54-55	5.678298282144421E-5	0.0	0.0	0.8254826127667452	0.0
56-57	5.678298282144421E-5	0.0	0.0	0.9674684613117664	0.0
58-59	5.678298282144421E-5	0.0	0.0	1.1184828041253971	0.0
60-61	5.678298282144421E-5	0.0	2.8391491410722105E-5	1.280186543455165	0.0
62-63	5.678298282144421E-5	0.0	2.8391491410722105E-5	1.4466884448333448	0.0
64-65	5.678298282144421E-5	0.0	2.8391491410722105E-5	1.6447900761516583	0.0
66-67	5.678298282144421E-5	0.0	2.8391491410722105E-5	1.8802691059121872	0.0
68-69	5.678298282144421E-5	0.0	2.8391491410722105E-5	2.1154358292671986	0.0
70-71	5.678298282144421E-5	0.0	2.8391491410722105E-5	2.37499084374402	0.0
72-73	5.678298282144421E-5	0.0	2.8391491410722105E-5	2.7014646034759133	0.0
74-75	5.678298282144421E-5	0.0	2.8391491410722105E-5	3.0185549752965635	0.0
76-77	5.678298282144421E-5	0.0	2.8391491410722105E-5	3.3377747089730176	0.0
78-79	5.678298282144421E-5	0.0	2.8391491410722105E-5	3.685840197922765	0.0
80-81	5.678298282144421E-5	0.0	2.8391491410722105E-5	4.069664770304317	0.0
82-83	5.678298282144421E-5	0.0	2.8391491410722105E-5	4.488538638832406	0.0
84-85	5.678298282144421E-5	0.0	2.8391491410722105E-5	4.904289443305315	0.0
86-87	5.678298282144421E-5	0.0	2.8391491410722105E-5	5.348133628529133	0.0
88	5.678298282144421E-5	0.0	2.8391491410722105E-5	5.706774948029375	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	605	0.0	62.970417	2
GGTATCA	11440	0.0	58.977222	1
ATAACGC	665	0.0	57.28887	3
TACCTGG	4225	0.0	46.643833	2
TAACGCA	875	0.0	43.539547	4
GTACCTG	4770	0.0	42.216923	1
GTATCAA	20435	0.0	39.855076	1
GTCTCGC	1430	0.0	39.81168	1
GTATAAC	1125	0.0	38.476585	1
TCTGTCG	1450	0.0	36.30866	8
TCTCGCT	1680	0.0	32.475483	2
TCGCTCT	1645	0.0	32.308697	4
ACCTGGG	6165	0.0	31.43193	3
CTCGCTC	1690	0.0	31.170103	3
TATCACG	410	0.0	30.973253	2
CGCTCTG	1560	0.0	30.133812	5
ATCAACG	27850	0.0	29.115145	3
TATCAAC	27865	0.0	29.082594	2
TCAACGC	28015	0.0	28.994034	4
CTGTCGC	1855	0.0	28.888243	9
>>END_MODULE