##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431440_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12859956
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.08154732411215	31.0	31.0	34.0	26.0	34.0
2	30.106412728006223	31.0	31.0	34.0	26.0	34.0
3	30.195837995091118	31.0	31.0	34.0	26.0	34.0
4	33.53264249115627	37.0	35.0	37.0	27.0	37.0
5	33.66800111913291	37.0	35.0	37.0	28.0	37.0
6	33.6779380116075	37.0	35.0	37.0	28.0	37.0
7	33.546376597244965	37.0	35.0	37.0	28.0	37.0
8	33.49183356459385	37.0	35.0	37.0	27.0	37.0
9	34.81251413301881	39.0	35.0	39.0	27.0	39.0
10-11	34.762715984409276	39.0	35.0	39.0	27.0	39.0
12-13	34.728839818736546	39.0	35.0	39.0	27.0	39.0
14-15	35.884262862174644	40.0	36.0	41.0	26.5	41.0
16-17	35.9094721241659	40.0	36.0	41.0	27.0	41.0
18-19	35.932228228463615	40.0	36.0	41.0	27.0	41.0
20-21	36.042648007504845	40.0	36.0	41.0	27.0	41.0
22-23	36.01522322471399	40.0	36.0	41.0	27.0	41.0
24-25	36.05992353317538	40.0	36.0	41.0	27.0	41.0
26-27	36.058678777750096	40.0	36.0	41.0	27.0	41.0
28-29	35.96022171459995	40.0	36.0	41.0	27.0	41.0
30-31	35.93803291395398	40.0	36.0	41.0	27.0	41.0
32-33	35.89065118107713	40.0	36.0	41.0	27.0	41.0
34-35	35.79922100822118	40.0	36.0	41.0	27.0	41.0
36-37	35.6841376828972	40.0	35.0	41.0	27.0	41.0
38-39	35.59870632527825	40.0	35.0	41.0	26.0	41.0
40-41	35.52349973048119	40.0	35.0	41.0	26.0	41.0
42-43	35.3026591615088	40.0	35.0	41.0	25.0	41.0
44-45	35.183467307353155	39.5	35.0	41.0	25.0	41.0
46-47	35.05494917712005	39.0	35.0	41.0	25.0	41.0
48-49	34.92423026175206	39.0	34.5	41.0	24.0	41.0
50-51	34.760170097005	39.0	34.0	41.0	24.0	41.0
52-53	34.67446988154548	39.0	34.0	41.0	24.0	41.0
54-55	34.593568749379855	39.0	34.0	41.0	24.0	41.0
56-57	34.430049916189446	38.5	34.0	41.0	23.5	41.0
58-59	34.22139333136132	38.0	33.5	40.0	23.0	41.0
60-61	34.0050580266371	38.0	33.0	40.0	23.0	41.0
62-63	33.734800958883525	37.5	33.0	40.0	22.0	41.0
64-65	33.59562559934108	37.0	33.0	40.0	22.0	41.0
66-67	33.338506640302654	37.0	33.0	40.0	21.0	41.0
68-69	33.080432312521125	36.0	33.0	39.5	20.0	41.0
70-71	32.797473529458415	36.0	32.5	39.0	19.5	41.0
72-73	32.48796749382346	35.0	32.0	39.0	18.5	41.0
74-75	32.15090354897015	35.0	32.0	38.5	17.5	40.0
76-77	31.836769970286056	35.0	32.0	37.0	15.5	40.0
78-79	31.508202710802433	35.0	32.0	37.0	12.5	39.0
80-81	31.20995351772588	35.0	31.0	36.5	9.0	39.0
82-83	30.913011794130554	35.0	31.0	36.0	7.0	38.5
84-85	30.62346076456249	35.0	31.0	36.0	2.0	37.0
86-87	30.36932276440137	35.0	31.0	35.0	2.0	37.0
88-89	30.10750841604746	35.0	31.0	35.0	2.0	36.5
90-91	29.89087727049766	35.0	31.0	35.0	2.0	36.0
92-93	29.711295435225438	35.0	30.5	35.0	2.0	36.0
94-95	29.48451398278501	34.0	30.0	35.0	2.0	36.0
96-97	29.38904635443543	34.5	30.0	35.0	2.0	35.5
98-99	29.24134176664368	34.0	30.0	35.0	2.0	35.0
100	29.10666583929214	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4050227139033993
1101	2	0.4661949152651417
1101	3	0.45728265210223284
1101	4	0.5024892085629133
1101	5	0.40535992161782985
1101	6	0.44398933213750524
1101	7	0.515260530284408
1101	8	0.5659399353034829
1101	9	0.6676399995496922
1101	10-11	0.7116761450785418
1101	12-13	0.6348283401826649
1101	14-15	0.7803116176287475
1101	16-17	0.7260614830181353
1101	18-19	0.6917032733887396
1101	20-21	0.7016614752779802
1101	22-23	0.6767707848651909
1101	24-25	0.635824234290574
1101	26-27	0.6941617018855197
1101	28-29	0.7638239533074866
1101	30-31	0.7230797964830344
1101	32-33	0.7262748453532382
1101	34-35	0.7033714773393527
1101	36-37	0.7081149134381803
1101	38-39	0.69463535850808
1101	40-41	0.6695428001805794
1101	42-43	0.7037158268388666
1101	44-45	0.6431500947144855
1101	46-47	0.6383166672767757
1101	48-49	0.6317310232349342
1101	50-51	0.5763045341616859
1101	52-53	0.5442639258165087
1101	54-55	0.6581702165027039
1101	56-57	0.6350012494479884
1101	58-59	0.6530381615387739
1101	60-61	0.6399747328344034
1101	62-63	0.6649112733618523
1101	64-65	0.6450704473626772
1101	66-67	0.6663045226739399
1101	68-69	0.6067333754354607
1101	70-71	0.6338638381247392
1101	72-73	0.5994210226468013
1101	74-75	0.6153698472136071
1101	76-77	0.6280619655036261
1101	78-79	0.6029827778900234
1101	80-81	0.5943159957830915
1101	82-83	0.585661393178956
1101	84-85	0.617425485865823
1101	86-87	0.6355991533203174
1101	88-89	0.6287090965624209
1101	90-91	0.619048087383522
1101	92-93	0.5862084717027969
1101	94-95	0.6186194470543747
1101	96-97	0.6311255145497867
1101	98-99	0.6560687198212989
1101	100	0.6365791929048363
1102	1	0.30034528113840153
1102	2	0.4083355512109641
1102	3	0.3955508615224588
1102	4	0.41126985218733836
1102	5	0.3868743559367189
1102	6	0.37840599562392185
1102	7	0.41218708856311537
1102	8	0.4123717275892673
1102	9	0.6264773731782469
1102	10-11	0.5753926615454361
1102	12-13	0.5986191044180131
1102	14-15	0.6785166920233507
1102	16-17	0.6037670356835037
1102	18-19	0.5834821596764783
1102	20-21	0.5838230707142955
1102	22-23	0.5619120244483256
1102	24-25	0.5743456279254673
1102	26-27	0.6192632784807515
1102	28-29	0.6532488648203199
1102	30-31	0.6466679969859896
1102	32-33	0.641267685844376
1102	34-35	0.6388392259508464
1102	36-37	0.5544264474455431
1102	38-39	0.5443340638687175
1102	40-41	0.5992218781998702
1102	42-43	0.6130895533672884
1102	44-45	0.5050502723734951
1102	46-47	0.5033430074097609
1102	48-49	0.4796990150334537
1102	50-51	0.46427433221289505
1102	52-53	0.46153978560102615
1102	54-55	0.4925973045763996
1102	56-57	0.5225863407493208
1102	58-59	0.5267123225032435
1102	60-61	0.5164371656177167
1102	62-63	0.5153703093871158
1102	64-65	0.4970219536953877
1102	66-67	0.503172814231803
1102	68-69	0.49114227531333654
1102	70-71	0.4827896059226333
1102	72-73	0.4898563651133472
1102	74-75	0.5283167320385616
1102	76-77	0.508709749156111
1102	78-79	0.45294363322687303
1102	80-81	0.4481029550381521
1102	82-83	0.45691879829040616
1102	84-85	0.45072517462319084
1102	86-87	0.440968525328028
1102	88-89	0.474990615274816
1102	90-91	0.4325251812646691
1102	92-93	0.40127334119575053
1102	94-95	0.4031938302903022
1102	96-97	0.43805391097262003
1102	98-99	0.48026325240090983
1102	100	0.5213910185528619
1103	1	0.22282971307742727
1103	2	0.26516086130774497
1103	3	0.24607313414349719
1103	4	0.25500366742271297
1103	5	0.24180386044432112
1103	6	0.25602592606903585
1103	7	0.31109275137239933
1103	8	0.3093734371722121
1103	9	0.3597781113515808
1103	10-11	0.4808634854691789
1103	12-13	0.4095132858663675
1103	14-15	0.4618253964069794
1103	16-17	0.4053701771080469
1103	18-19	0.35081527280168245
1103	20-21	0.36846413857711013
1103	22-23	0.32512551410873414
1103	24-25	0.4072464606413604
1103	26-27	0.3834378953549731
1103	28-29	0.46542830072650787
1103	30-31	0.3530762121860036
1103	32-33	0.4475170296130102
1103	34-35	0.41957760443080616
1103	36-37	0.43890573730036664
1103	38-39	0.41158170346695755
1103	40-41	0.41644882576824216
1103	42-43	0.3736753234039796
1103	44-45	0.31186191262574425
1103	46-47	0.32012065904566356
1103	48-49	0.31488327422268725
1103	50-51	0.23417159638764673
1103	52-53	0.25346636571212144
1103	54-55	0.32049858918787066
1103	56-57	0.3125706071633445
1103	58-59	0.3340445987880116
1103	60-61	0.3530000370069075
1103	62-63	0.3018795926974107
1103	64-65	0.341446398625493
1103	66-67	0.3689505416923282
1103	68-69	0.28742110589102055
1103	70-71	0.31251778993889445
1103	72-73	0.28491328212312084
1103	74-75	0.2708692375870001
1103	76-77	0.2691261206798785
1103	78-79	0.3027826830960585
1103	80-81	0.30283838755501336
1103	82-83	0.27938799959505545
1103	84-85	0.257162183521082
1103	86-87	0.2128786665796092
1103	88-89	0.2504027130069524
1103	90-91	0.2864359775032739
1103	92-93	0.22455334335869281
1103	94-95	0.24626976777603815
1103	96-97	0.21021921131915278
1103	98-99	0.22688180212558606
1103	100	0.2705220105077437
1104	1	0.2341222270830272
1104	2	0.3017410150488615
1104	3	0.30532710685782405
1104	4	0.34825720795569737
1104	5	0.24189257602488823
1104	6	0.29305963039846006
1104	7	0.3397179546709168
1104	8	0.2968765901754793
1104	9	0.40133548388588025
1104	10-11	0.42961365098378224
1104	12-13	0.39160126367480785
1104	14-15	0.45650277705835407
1104	16-17	0.5042075755619564
1104	18-19	0.386718333686602
1104	20-21	0.3590937898999442
1104	22-23	0.4127073619863282
1104	24-25	0.44204509127997227
1104	26-27	0.5071746186136821
1104	28-29	0.5508295882588357
1104	30-31	0.49132708333651465
1104	32-33	0.4962781807163168
1104	34-35	0.46695488907722194
1104	36-37	0.42164857323176363
1104	38-39	0.48776401383983625
1104	40-41	0.4797799433246013
1104	42-43	0.44169043432301436
1104	44-45	0.43890546475873293
1104	46-47	0.44820379942600397
1104	48-49	0.4165712763173559
1104	50-51	0.3479900791112769
1104	52-53	0.38125779247863534
1104	54-55	0.38502414382727324
1104	56-57	0.423421360238045
1104	58-59	0.44498552760970256
1104	60-61	0.40547947541361395
1104	62-63	0.43822812663041333
1104	64-65	0.4038407370526542
1104	66-67	0.41623421096449675
1104	68-69	0.4144027070429175
1104	70-71	0.37463648356278156
1104	72-73	0.4106015569087518
1104	74-75	0.44941300226633274
1104	76-77	0.38752318280044307
1104	78-79	0.4188317710661913
1104	80-81	0.43351798863095325
1104	82-83	0.37552157184586576
1104	84-85	0.3567828314437236
1104	86-87	0.3976486549228788
1104	88-89	0.38196584599555905
1104	90-91	0.3608205521799768
1104	92-93	0.3595986815404544
1104	94-95	0.3822842488524145
1104	96-97	0.4101384431760131
1104	98-99	0.4483265897972437
1104	100	0.43011009415033286
1105	1	0.09687955980001917
1105	2	0.1488175083359664
1105	3	0.18105476891865635
1105	4	0.16379742811550102
1105	5	0.0945266483715983
1105	6	0.21488678989633314
1105	7	0.22398616584641218
1105	8	0.1786769698179782
1105	9	0.32282040684821567
1105	10-11	0.34325785808200493
1105	12-13	0.26623574087518165
1105	14-15	0.3421352915212097
1105	16-17	0.33334480264660016
1105	18-19	0.31473177237381833
1105	20-21	0.2792086326189249
1105	22-23	0.2801720881700547
1105	24-25	0.3310378903159261
1105	26-27	0.3351919651052384
1105	28-29	0.42864973802313244
1105	30-31	0.39006856043966565
1105	32-33	0.40953754946980325
1105	34-35	0.3709424577229967
1105	36-37	0.3627431037831528
1105	38-39	0.3356501421189364
1105	40-41	0.33591896433901525
1105	42-43	0.401423518430299
1105	44-45	0.3401192879638444
1105	46-47	0.28765401205114927
1105	48-49	0.2671188972177063
1105	50-51	0.30722209928833877
1105	52-53	0.31452007675931526
1105	54-55	0.3819477107307847
1105	56-57	0.3480979756445137
1105	58-59	0.3473011403782209
1105	60-61	0.33754530611584244
1105	62-63	0.3723412242856199
1105	64-65	0.4139751091582866
1105	66-67	0.3966231362770003
1105	68-69	0.3924734789818274
1105	70-71	0.37843780795115833
1105	72-73	0.33668288563494997
1105	74-75	0.3538119767036605
1105	76-77	0.3211028898642567
1105	78-79	0.36089253810887456
1105	80-81	0.3936690524955466
1105	82-83	0.3217913678861848
1105	84-85	0.2520085879647702
1105	86-87	0.3030101919640522
1105	88-89	0.3399269918081238
1105	90-91	0.33233444767524745
1105	92-93	0.3170541683339927
1105	94-95	0.30134557022902797
1105	96-97	0.3691268185621617
1105	98-99	0.37351911480541133
1105	100	0.3268414471387828
1106	1	0.08913582334047021
1106	2	0.13764701589457928
1106	3	0.08900240948725369
1106	4	0.10417551180773188
1106	5	0.11011374548643005
1106	6	0.09482369459002626
1106	7	0.21974536140793788
1106	8	0.15712849818100239
1106	9	0.2477670732876618
1106	10-11	0.252199998555632
1106	12-13	0.2143350494719769
1106	14-15	0.2693228810547481
1106	16-17	0.2169025358973684
1106	18-19	0.21759864387298222
1106	20-21	0.2375316673099661
1106	22-23	0.25369752051220473
1106	24-25	0.2250178707621302
1106	26-27	0.28152579973631475
1106	28-29	0.36361860623694753
1106	30-31	0.2892172352431217
1106	32-33	0.30678722564148586
1106	34-35	0.2593094211177345
1106	36-37	0.2820445020202982
1106	38-39	0.24812344380949725
1106	40-41	0.2549725950473274
1106	42-43	0.20509978107882176
1106	44-45	0.1779949504815761
1106	46-47	0.20164122608140644
1106	48-49	0.17062600407044215
1106	50-51	0.21151647600814982
1106	52-53	0.2499698149973355
1106	54-55	0.3076174865223251
1106	56-57	0.27833838532730937
1106	58-59	0.2513126303246409
1106	60-61	0.2641828045476018
1106	62-63	0.2963987988943657
1106	64-65	0.32445363113445325
1106	66-67	0.31292006128417427
1106	68-69	0.3023866738535048
1106	70-71	0.3050964547654189
1106	72-73	0.26179496093424603
1106	74-75	0.2887464516384384
1106	76-77	0.31768180648163735
1106	78-79	0.2764319565106561
1106	80-81	0.215208363189479
1106	82-83	0.17503157669379377
1106	84-85	0.24969727535954434
1106	86-87	0.27951499873545416
1106	88-89	0.29496248235489375
1106	90-91	0.26634866062825324
1106	92-93	0.24382230081725353
1106	94-95	0.3000581472097217
1106	96-97	0.3418138508085313
1106	98-99	0.37618084884898906
1106	100	0.32577262982522015
1107	1	0.14672790335968955
1107	2	0.15795788074361283
1107	3	0.21354225609938737
1107	4	0.224078094383529
1107	5	0.1583900376080294
1107	6	0.21787180711892518
1107	7	0.25269151594687855
1107	8	0.3137774778524758
1107	9	0.3903015630984399
1107	10-11	0.3950524442338832
1107	12-13	0.3208464368433681
1107	14-15	0.41686594496908924
1107	16-17	0.37393906017197764
1107	18-19	0.3740961248401504
1107	20-21	0.3670059054125616
1107	22-23	0.4127489988219608
1107	24-25	0.4337654612450592
1107	26-27	0.4264097884514797
1107	28-29	0.4660411307779029
1107	30-31	0.4375734863533154
1107	32-33	0.4891748072225113
1107	34-35	0.4786242908070122
1107	36-37	0.46533140599873946
1107	38-39	0.4875588585436148
1107	40-41	0.5041572668777405
1107	42-43	0.4895417389689598
1107	44-45	0.49416022662124703
1107	46-47	0.4723329432183405
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2216	54-55	-1.681900593867681
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2216	84-85	-1.477768625724437
2216	86-87	-1.5442739504711263
2216	88-89	-1.5170091133000234
2216	90-91	-1.6946848728182289
2216	92-93	-1.6046669665030606
2216	94-95	-1.5207382782422592
2216	96-97	-1.6049716956119333
2216	98-99	-1.8832365732971397
2216	100	-1.9102024106124276
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	391546.0
3	77125.0
4	48841.0
5	39787.0
6	29959.0
7	29384.0
8	32420.0
9	35054.0
10	35501.0
11	36587.0
12	39170.0
13	41164.0
14	43199.0
15	43856.0
16	44795.0
17	45247.0
18	44689.0
19	42484.0
20	42497.0
21	46585.0
22	57379.0
23	82873.0
24	118028.0
25	154167.0
26	170617.0
27	148910.0
28	154674.0
29	188765.0
30	221498.0
31	253972.0
32	318994.0
33	416692.0
34	573300.0
35	839015.0
36	1392416.0
37	2366865.0
38	2964824.0
39	1242647.0
40	4430.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.09733411322944	21.586004426929186	14.857285157405945	21.459376302435434
2	15.149889116046044	21.590871533024615	40.33553623669884	22.9237031142305
3	17.059780678427202	29.211364518507498	30.161238506086136	23.567616296979164
4	11.75118667492973	19.11894932267938	39.61712998905266	29.512734013338225
5	12.188851114760205	41.13135222005204	34.48146551003434	12.19833115515342
6	27.835623921371518	40.36588540218596	18.745720191163045	13.05277048527948
7	25.68975392608878	35.38911703393894	22.82984803478779	16.091281005184495
8	25.000114706536742	37.30324232218601	20.265837648178486	17.430805323098763
9	25.97164208632422	18.39261762294215	21.23084307277299	34.40489721796063
10-11	23.175972473935744	29.559832600842128	28.331879756607876	18.932315168614252
12-13	23.67616343939185	27.542660238233594	28.22080302791407	20.560373294460483
14-15	21.589122832366908	28.88221223294467	25.826344075025396	23.702320859663022
16-17	20.64164016009509	31.986586291211395	26.737381099532964	20.634392449160554
18-19	20.86110133964158	30.941313882486078	28.375226400099507	19.822358377772836
20-21	22.790602077177226	29.41059936782915	27.753009483806977	20.045789071186643
22-23	22.262722593106236	29.617817021455128	27.708945422211894	20.410514963226746
24-25	21.405110375266926	29.906428133080453	27.845456545798292	20.84300494585433
26-27	21.16227133063429	30.05234105000784	27.929882932140863	20.855504687217007
28-29	21.239213116396265	29.847853387122473	28.358352139597713	20.554581356883546
30-31	21.503786961362298	29.889864356526076	27.89284487119852	20.713503810913107
32-33	21.21012676793082	29.932239598143166	27.935067377602184	20.922566256323822
34-35	21.651011535970667	29.455261641886594	28.05608271582742	20.837644106315327
36-37	21.49646997598617	29.70712024299465	27.798596451362634	20.997813329656545
38-39	21.30079355411139	29.82979038271783	28.014650397243653	20.85476566592713
40-41	21.41787757155683	29.429905550001806	28.081099229850103	21.071117648591258
42-43	21.22837348581059	29.78199467419348	28.15764545942838	20.83198638056755
44-45	21.277004669753914	29.647353870918824	27.973610641284356	21.102030818042902
46-47	21.361348841737815	29.329944136024533	28.097637064887632	21.21106995735002
48-49	21.109697364030463	29.507934363875638	28.108526940085977	21.273841332007926
50-51	21.1902419260926	29.467796034846877	28.015842882280108	21.32611915678042
52-53	21.314084182516737	29.555680616471342	27.893401234308207	21.23683396670371
54-55	21.178942723829948	29.71870922949545	27.94737236248833	21.154975684186272
56-57	21.308445568937902	29.37711859118209	27.956626058743954	21.35780978113605
58-59	21.496403538963076	29.06944671469206	28.121616823434287	21.31253292291058
60-61	21.329775275787437	29.442924896249462	28.09025439733003	21.13704543063308
62-63	21.042372299116888	29.446300468965507	28.150928717965883	21.36039851395172
64-65	21.42807817810965	29.365934530044125	27.888210581864353	21.31777670998187
66-67	21.051535315638517	29.219128964305146	28.081640670655787	21.647695049400546
68-69	21.3299576548418	29.422137722254536	28.168953242479972	21.078951380423696
70-71	21.297052454675438	29.25110069809414	28.109764464430615	21.342082382799806
72-73	21.146308276519168	29.181920740959487	28.176554492510135	21.495216490011213
74-75	21.16355750609848	29.20001460392851	28.1145025758476	21.521925314125408
76-77	21.168653662012403	28.992286720506442	27.948854143746814	21.89020547373434
78-79	21.226400513644826	28.96615602999797	28.201890443458804	21.605553012898394
80-81	21.01347852224187	29.346556205703312	28.12774592749754	21.512219344557277
82-83	21.05612085247321	29.257218202087266	28.27260050613886	21.414060439300663
84-85	21.033079497960333	28.923425696240578	28.205859576378906	21.83763522942018
86-87	21.3673114407811	28.991867861458147	28.01126147528452	21.62955922247623
88-89	21.09768358877154	29.118287648812295	28.105709476781275	21.678319285634885
90-91	21.035707900818927	29.058735227271228	28.043174156938584	21.86238271497126
92-93	21.085800931350608	29.031352753941725	28.102074527333816	21.780771787373858
94-95	21.158199724946726	28.73815664537183	28.319954512124536	21.78368911755691
96-97	21.39430226914969	28.94790497887556	27.991898910017778	21.665893841956972
98-99	21.027336304710527	28.95971043166775	28.057227802513335	21.955725461108386
100	20.906066624467126	28.692216051273995	28.33664345691992	22.065073867338956
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21306.0
1	18230.5
2	14075.5
3	11553.0
4	8973.5
5	7139.5
6	6173.0
7	5941.0
8	6118.0
9	6180.0
10	5767.0
11	5391.0
12	5421.5
13	5619.5
14	5992.0
15	6546.5
16	7490.5
17	8770.5
18	10833.5
19	14040.0
20	18682.5
21	25474.0
22	36769.5
23	53481.0
24	73811.5
25	95719.5
26	122588.0
27	152556.0
28	187279.0
29	224589.0
30	250859.5
31	273178.5
32	301813.0
33	327062.0
34	344622.0
35	362810.5
36	376621.0
37	389272.5
38	403095.5
39	407832.5
40	420835.0
41	439023.0
42	451713.5
43	479001.5
44	494561.5
45	487659.5
46	484747.0
47	480252.5
48	467760.0
49	450510.0
50	440709.0
51	411576.0
52	359198.5
53	317717.0
54	294816.5
55	282338.0
56	249518.5
57	209396.5
58	182687.0
59	163165.0
60	139538.0
61	113670.0
62	96245.0
63	79220.0
64	62255.0
65	48971.5
66	38825.5
67	29669.0
68	21915.5
69	16431.5
70	11945.0
71	8643.5
72	6506.5
73	4853.0
74	3673.0
75	2681.0
76	1870.0
77	1391.0
78	1036.5
79	775.0
80	582.0
81	428.5
82	357.0
83	310.5
84	253.5
85	222.5
86	196.5
87	153.5
88	119.5
89	100.0
90	82.0
91	76.5
92	72.5
93	67.0
94	61.0
95	62.5
96	57.5
97	55.0
98	67.5
99	79.5
100	133.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014253547990366374
2	0.007908269670596072
3	0.010132227513064586
4	0.008763638071545501
5	0.010583239942656102
6	0.012542811188467519
7	0.011049804524992154
8	0.008203760572742238
9	0.006998468735040774
10-11	0.006772962520245014
12-13	0.007297847675373072
14-15	0.008219312725486774
16-17	0.005625991255335555
18-19	0.006279181670606027
20-21	0.006018683112135065
22-23	0.0063336142052119
24-25	0.006240301288744689
26-27	0.006477471618098848
28-29	0.016290879999900466
30-31	0.0059681386157153255
32-33	0.005828169241014511
34-35	0.0054432534605872674
36-37	0.007056789307832779
38-39	0.006084779761299339
40-41	0.005995354883018262
42-43	0.005311060162258721
44-45	0.00471230228159412
46-47	0.006718529985639142
48-49	0.00398912717897324
50-51	0.0043040582720500755
52-53	0.003460353985659049
54-55	0.0048911520381562735
56-57	0.0034020334128670427
58-59	0.0028771482577389846
60-61	0.0039269185679951
62-63	0.0037441807732468135
64-65	0.005077777871090694
66-67	0.0037558448878052147
68-69	0.004016343446276177
70-71	0.005680423789941427
72-73	0.003215407579932622
74-75	0.0031298707398376793
76-77	0.0029587970596477936
78-79	0.003783061155108151
80-81	0.00444402764675089
82-83	0.0018973626348332763
84-85	0.0029121406014141885
86-87	0.0028149396467608443
88-89	0.002002339665858888
90-91	0.002838267875877647
92-93	0.002737178883038169
94-95	0.002064548276837028
96-97	0.0015940956563148428
98-99	0.0020412200477202256
100	0.0032348477708632906
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2859956E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.21163213462361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.10945843235557	22.926102988046722
2	14.856572227182111	10.462483120900048
3	6.659053498918249	7.034284265060629
4	3.763401138141794	5.300619858050907
5	2.3069648211441525	4.061599831482283
6	1.586013568314278	3.350767579680246
7	1.0863432181988735	2.6776342439813323
8	0.8255992917365528	2.325655884098664
9	0.6089223817446947	1.9297035814079737
>10	2.874369872090253	17.43013587727923
>50	0.17023465593723366	4.152217936598018
>100	0.12907679556424015	9.37939831907103
>500	0.01614965491745574	3.894534562589589
>1k	0.0077290753266686125	4.644300876843697
>5k	8.909474252283259E-5	0.2047474848189877
>10k+	2.2273685630708148E-5	0.22581359009071836
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28792	0.2238887909103266	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.776076372267525E-6	0.0
8	0.0	0.0	0.0	7.776076372267525E-6	0.0
9	0.0	0.0	0.0	7.776076372267525E-6	0.0
10-11	0.0	0.0	0.0	2.3328229116802578E-5	0.0
12-13	0.0	7.776076372267525E-6	0.0	2.3328229116802578E-5	0.0
14-15	0.0	7.776076372267525E-6	0.0	3.11043054890701E-5	0.0
16-17	0.0	7.776076372267525E-6	0.0	3.888038186133763E-5	0.0
18-19	0.0	7.776076372267525E-6	0.0	6.22086109781402E-5	0.0
20-21	0.0	1.555215274453505E-5	0.0	9.720095465334407E-5	0.0
22-23	0.0	1.555215274453505E-5	0.0	2.682746348432296E-4	0.0
24-25	0.0	1.555215274453505E-5	0.0	5.132210405696567E-4	0.0
26-27	0.0	1.9440190930668814E-5	0.0	9.52569355602772E-4	0.0
28-29	0.0	2.3328229116802578E-5	0.0	0.002414471713589067	0.0
30-31	0.0	2.3328229116802578E-5	0.0	0.00678851467298955	0.0
32-33	0.0	3.11043054890701E-5	0.0	0.014210779570318903	0.0
34-35	0.0	3.888038186133763E-5	0.0	0.025657163990296702	0.0
36-37	0.0	3.888038186133763E-5	0.0	0.04587107452000613	0.0
38-39	0.0	3.888038186133763E-5	0.0	0.07940540387540984	0.0
40-41	0.0	3.888038186133763E-5	0.0	0.11989543354580684	0.0
42-43	0.0	3.888038186133763E-5	0.0	0.15920738764580533	0.0
44-45	0.0	3.888038186133763E-5	0.0	0.20757847071949548	0.0
46-47	0.0	3.888038186133763E-5	0.0	0.2680063602083864	0.0
48-49	7.776076372267525E-6	3.888038186133763E-5	0.0	0.3377344370385093	0.0
50-51	7.776076372267525E-6	3.888038186133763E-5	0.0	0.4145309672910234	0.0
52-53	7.776076372267525E-6	3.888038186133763E-5	0.0	0.49241614823565494	0.0
54-55	7.776076372267525E-6	3.888038186133763E-5	0.0	0.5768099051038744	0.0
56-57	7.776076372267525E-6	3.888038186133763E-5	0.0	0.6769346644731911	0.0
58-59	7.776076372267525E-6	3.888038186133763E-5	0.0	0.7929926043292839	0.0
60-61	7.776076372267525E-6	3.888038186133763E-5	0.0	0.9239222902473383	0.0
62-63	7.776076372267525E-6	3.888038186133763E-5	0.0	1.0603107817787247	0.0
64-65	7.776076372267525E-6	4.2768420047471396E-5	0.0	1.1972319345416111	0.0
66-67	7.776076372267525E-6	4.6656458233605156E-5	0.0	1.3547246973473315	0.0
68-69	7.776076372267525E-6	4.6656458233605156E-5	0.0	1.5437338976898523	0.0
70-71	7.776076372267525E-6	4.6656458233605156E-5	0.0	1.7555503300322335	0.0
72-73	7.776076372267525E-6	4.6656458233605156E-5	0.0	1.9783426941740703	0.0
74-75	7.776076372267525E-6	4.6656458233605156E-5	0.0	2.1972470201297734	0.0
76-77	7.776076372267525E-6	4.6656458233605156E-5	0.0	2.4282859132644	0.0
78-79	7.776076372267525E-6	4.6656458233605156E-5	3.888038186133763E-6	2.7016772063605816	0.0
80-81	7.776076372267525E-6	5.4432534605872676E-5	7.776076372267525E-6	3.011071733060362	0.0
82-83	7.776076372267525E-6	5.4432534605872676E-5	7.776076372267525E-6	3.3370059742039553	0.0
84-85	7.776076372267525E-6	5.4432534605872676E-5	7.776076372267525E-6	3.659285459452583	0.0
86-87	7.776076372267525E-6	5.4432534605872676E-5	7.776076372267525E-6	3.99142112150306	0.0
88	7.776076372267525E-6	5.4432534605872676E-5	7.776076372267525E-6	4.261577566828378	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20555	0.0	46.5401	1
GTATCAA	35375	0.0	35.91947	1
ATCAACG	42440	0.0	28.919441	3
TCAACGC	42785	0.0	28.57616	4
TATCAAC	46385	0.0	27.017555	2
GTACATG	82080	0.0	26.992336	1
TACATGG	81080	0.0	26.193647	2
CAACGCA	47875	0.0	25.567238	5
CATGGGT	25745	0.0	25.013973	4
AACGCAG	49295	0.0	24.781334	6
ACATGGG	84765	0.0	24.311535	3
ACGCAGA	52285	0.0	22.797823	7
CGCAGAG	52495	0.0	22.517544	8
ATGGGTG	16180	0.0	20.161953	5
CATGGGA	34170	0.0	20.139612	4
CATGGGG	29400	0.0	19.92167	4
GCAGAGT	57590	0.0	19.709291	9
TACCTGG	8095	0.0	19.685493	2
GTGGTAT	10465	0.0	19.31566	1
TAGCGTG	2245	0.0	19.051361	9
>>END_MODULE