FastQCFastQC Report
Wed 25 May 2016
SRR2431440_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431440_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12859956
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT934990.7270553647306414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT722170.5615649073760439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT627370.48784770336694777No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149760.11645451975107846No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147190.11445606812340572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA338950.060.2827151
GTATCAA679600.044.5831871
ATCAACG937800.032.022573
TATCAAC952450.031.6287652
TCAACGC955100.031.447464
CAACGCA999650.030.0668665
GTGGTAT118700.029.4494171
AACGCAG1028500.029.240966
TGGTATC123650.027.6108632
ACGCAGA1134950.026.2540157
CGCAGAG1140050.026.049988
GCAGAGT1216000.024.1446049
GTACATG799950.024.1193471
TACATGG790100.023.5099962
CATGGGT248150.022.8165364
ACATGGG811000.022.2025093
AGTACTT763200.022.17688412-13
AGAGTAC1185950.022.15629410-11
GAGTACT760550.020.6383112-13
CAGAGTA1202550.020.39831210-11
Produced by FastQC (version 0.11.5)