##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431440_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12859956
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27452706681112	33.0	31.0	34.0	30.0	34.0
2	32.39237793659636	34.0	31.0	34.0	31.0	34.0
3	32.48725703260571	34.0	31.0	34.0	31.0	34.0
4	36.041370048233446	37.0	35.0	37.0	35.0	37.0
5	35.96364303268223	37.0	35.0	37.0	35.0	37.0
6	36.0128008214025	37.0	35.0	37.0	35.0	37.0
7	35.95011880289481	37.0	35.0	37.0	35.0	37.0
8	35.933047826913246	37.0	35.0	37.0	35.0	37.0
9	37.61169322818834	39.0	37.0	39.0	35.0	39.0
10-11	37.547263108831785	39.0	37.0	39.0	35.0	39.0
12-13	37.53106872993966	39.0	37.0	39.0	35.0	39.0
14-15	38.85001223954421	40.0	38.0	41.0	35.0	41.0
16-17	38.91899233558809	40.0	38.0	41.0	35.0	41.0
18-19	39.012608013588846	40.5	38.5	41.0	35.0	41.0
20-21	39.03228195337527	41.0	39.0	41.0	35.0	41.0
22-23	38.98813129687224	40.5	39.0	41.0	35.0	41.0
24-25	38.94469942198869	41.0	38.0	41.0	35.0	41.0
26-27	38.81671966840322	40.0	38.0	41.0	35.0	41.0
28-29	38.734017519189024	40.0	38.0	41.0	34.5	41.0
30-31	38.55217673373066	40.0	38.0	41.0	34.0	41.0
32-33	38.362666248624805	40.0	38.0	41.0	34.0	41.0
34-35	38.23430185142158	40.0	38.0	41.0	34.0	41.0
36-37	38.10891981278941	40.0	38.0	41.0	33.5	41.0
38-39	38.01873894436342	40.0	37.0	41.0	33.0	41.0
40-41	37.90610434436945	40.0	37.0	41.0	33.0	41.0
42-43	37.77522298676605	40.0	37.0	41.0	33.0	41.0
44-45	37.54866956776524	40.0	37.0	41.0	33.0	41.0
46-47	37.21324944657664	40.0	37.0	41.0	31.5	41.0
48-49	36.95306204780172	40.0	37.0	41.0	31.0	41.0
50-51	36.812744849204776	40.0	36.5	41.0	31.0	41.0
52-53	36.668824255697295	40.0	36.0	41.0	31.0	41.0
54-55	36.50431832737219	40.0	36.0	41.0	30.5	41.0
56-57	36.27697427580623	39.5	35.0	41.0	30.0	41.0
58-59	36.09595596594576	39.0	35.0	41.0	30.0	41.0
60-61	35.88185507788674	39.0	35.0	41.0	29.5	41.0
62-63	35.65081509610142	39.0	35.0	41.0	29.0	41.0
64-65	35.401901141807954	38.0	35.0	41.0	29.0	41.0
66-67	35.1242296241138	37.5	35.0	40.0	28.5	41.0
68-69	34.827478880954175	37.0	35.0	40.0	28.0	41.0
70-71	34.49817312749748	36.5	34.5	39.5	28.0	41.0
72-73	34.17146563331943	36.0	34.0	39.0	28.0	41.0
74-75	33.86085018486844	35.5	34.0	39.0	27.5	41.0
76-77	33.51397162634149	35.0	34.0	37.5	27.0	40.0
78-79	33.20745004881821	35.0	34.0	37.0	26.5	39.0
80-81	32.91020035371817	35.0	34.0	37.0	26.0	39.0
82-83	32.66709376766141	35.0	34.0	36.0	26.0	39.0
84-85	32.446226021302095	35.0	34.0	36.0	26.0	37.0
86-87	32.216005482444885	35.0	33.5	36.0	26.0	37.0
88-89	32.07393610055898	35.0	34.0	35.0	26.0	37.0
90-91	31.8934916262544	35.0	33.0	35.0	26.0	36.0
92-93	31.7122002983525	35.0	33.0	35.0	25.0	36.0
94-95	31.56088345092316	35.0	33.0	35.0	25.0	36.0
96-97	31.403587228447748	35.0	33.0	35.0	24.5	36.0
98-99	31.249772238723054	35.0	33.0	35.0	24.0	35.5
100	31.147408980248454	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17856873047629307
1101	2	0.2420647459212546
1101	3	0.22294341736948553
1101	4	0.17316672177317116
1101	5	0.1573860802152538
1101	6	0.1678736306854205
1101	7	0.19763280905596048
1101	8	0.2399069824233635
1101	9	0.33066836014473466
1101	10-11	0.30182168789261254
1101	12-13	0.32956300445488296
1101	14-15	0.4345942312323601
1101	16-17	0.4023878970856316
1101	18-19	0.39235561664715846
1101	20-21	0.3591748777809869
1101	22-23	0.35285016646028566
1101	24-25	0.3865994317206045
1101	26-27	0.3700528661882103
1101	28-29	0.38015508143556076
1101	30-31	0.3878830820850112
1101	32-33	0.4248151236460558
1101	34-35	0.444812022474693
1101	36-37	0.45526640719050704
1101	38-39	0.4475107188019365
1101	40-41	0.48314277784587034
1101	42-43	0.5025520527981584
1101	44-45	0.5564883880770424
1101	46-47	0.5525660661483016
1101	48-49	0.5573076717703174
1101	50-51	0.547649212158035
1101	52-53	0.5649836187584754
1101	54-55	0.5612983994484253
1101	56-57	0.5699208603118393
1101	58-59	0.5780719036437318
1101	60-61	0.5931428538304147
1101	62-63	0.5498252915707766
1101	64-65	0.5304928778227875
1101	66-67	0.5257113577746537
1101	68-69	0.5170282372306829
1101	70-71	0.5345789463312158
1101	72-73	0.5586488135812857
1101	74-75	0.5041863640082198
1101	76-77	0.5319848894860897
1101	78-79	0.5375507777695745
1101	80-81	0.5777459375572747
1101	82-83	0.5478996713244868
1101	84-85	0.5461815218450425
1101	86-87	0.5411219979978057
1101	88-89	0.5714411588611483
1101	90-91	0.5921040902477657
1101	92-93	0.6024380095387087
1101	94-95	0.6173123955514939
1101	96-97	0.6596282343038276
1101	98-99	0.7196769644509402
1101	100	0.75676627552129
1102	1	0.1360987330561798
1102	2	0.23117233853359664
1102	3	0.20152351946310176
1102	4	0.18924304061179953
1102	5	0.16365368104254685
1102	6	0.16311771653418106
1102	7	0.20593011603283884
1102	8	0.23098221736810842
1102	9	0.2667619322503114
1102	10-11	0.26489897016085706
1102	12-13	0.2894333968285068
1102	14-15	0.38210571994439846
1102	16-17	0.3625547256932151
1102	18-19	0.36882591944258536
1102	20-21	0.3440928900558262
1102	22-23	0.3295231695640837
1102	24-25	0.3419310191934315
1102	26-27	0.3522437442463513
1102	28-29	0.3618816397450857
1102	30-31	0.3532317207468978
1102	32-33	0.36624796680801097
1102	34-35	0.4133151361517804
1102	36-37	0.42674600995552225
1102	38-39	0.4389270557745846
1102	40-41	0.47149675300016725
1102	42-43	0.5065238110701316
1102	44-45	0.49752246676777645
1102	46-47	0.46191516424840984
1102	48-49	0.46311661002224724
1102	50-51	0.4462142789214454
1102	52-53	0.4448726337153559
1102	54-55	0.4158797251773194
1102	56-57	0.48577504522049253
1102	58-59	0.4466073983912935
1102	60-61	0.4651211046889969
1102	62-63	0.4514144118986181
1102	64-65	0.44218995030631447
1102	66-67	0.4346769628370595
1102	68-69	0.46070081210006464
1102	70-71	0.4842394865937223
1102	72-73	0.444235818973425
1102	74-75	0.4724743853607265
1102	76-77	0.4762488311682418
1102	78-79	0.43615179945974347
1102	80-81	0.44791421455770575
1102	82-83	0.45400622691980175
1102	84-85	0.4438020122848272
1102	86-87	0.48869821061660446
1102	88-89	0.4541704055594664
1102	90-91	0.4425387660765878
1102	92-93	0.4605001995700988
1102	94-95	0.5143643559364506
1102	96-97	0.5015987125436538
1102	98-99	0.5085440402100403
1102	100	0.4987813504964542
1103	1	0.12189997228614402
1103	2	0.19257316251247403
1103	3	0.14847001432178075
1103	4	0.13643791879783862
1103	5	0.11603754926394316
1103	6	0.13854496737276634
1103	7	0.12118183252772496
1103	8	0.1234269451247556
1103	9	0.2453534780827269
1103	10-11	0.230755721632093
1103	12-13	0.22498191938131384
1103	14-15	0.2640240405648413
1103	16-17	0.30500618613037744
1103	18-19	0.2900850398426442
1103	20-21	0.30369420879024034
1103	22-23	0.2660815797832825
1103	24-25	0.29204912664435057
1103	26-27	0.31539415586241404
1103	28-29	0.262085131522646
1103	30-31	0.2845117532523673
1103	32-33	0.3108800038493911
1103	34-35	0.3373332491071963
1103	36-37	0.3371307842391218
1103	38-39	0.3430612968555664
1103	40-41	0.34334945326560984
1103	42-43	0.3848763054585831
1103	44-45	0.39953167755199104
1103	46-47	0.38542403979352713
1103	48-49	0.346189756618017
1103	50-51	0.3017419560313357
1103	52-53	0.35351737733554245
1103	54-55	0.35945719656455566
1103	56-57	0.40507228404851503
1103	58-59	0.39732517856658944
1103	60-61	0.3866804613396866
1103	62-63	0.3669242171216993
1103	64-65	0.3676576682712209
1103	66-67	0.3608926249595754
1103	68-69	0.3484048312620516
1103	70-71	0.4003498017851115
1103	72-73	0.36903410349317056
1103	74-75	0.3554224871974583
1103	76-77	0.3501604814244814
1103	78-79	0.34255266593487477
1103	80-81	0.31957194506481557
1103	82-83	0.32308660213270457
1103	84-85	0.38068749811481695
1103	86-87	0.373464872562721
1103	88-89	0.36493264428103345
1103	90-91	0.38703826351262904
1103	92-93	0.390686707919329
1103	94-95	0.4155364323070856
1103	96-97	0.4003420134357576
1103	98-99	0.4363196232366704
1103	100	0.47234853928890175
1104	1	0.07986606061587764
1104	2	0.1513335674232721
1104	3	0.10947927655951162
1104	4	0.10268982905080293
1104	5	0.09296855006505922
1104	6	0.09594108545804403
1104	7	0.11698849975766734
1104	8	0.12455239004172824
1104	9	0.2070156050230807
1104	10-11	0.2265500025744842
1104	12-13	0.2156355741093563
1104	14-15	0.3186098137243789
1104	16-17	0.27141431744044553
1104	18-19	0.26942458657409674
1104	20-21	0.27175937773245806
1104	22-23	0.2451492228644554
1104	24-25	0.2662802811032847
1104	26-27	0.2522527203473288
1104	28-29	0.2893786434891794
1104	30-31	0.27541355810100043
1104	32-33	0.28443085615705144
1104	34-35	0.29536523198414955
1104	36-37	0.2908669742160157
1104	38-39	0.27225522995505
1104	40-41	0.320594794676488
1104	42-43	0.32251558017443216
1104	44-45	0.348197132474084
1104	46-47	0.3878303577834856
1104	48-49	0.36901132182001106
1104	50-51	0.347235734861016
1104	52-53	0.3489774281353206
1104	54-55	0.3757522296085227
1104	56-57	0.39772023995635664
1104	58-59	0.40152763667889246
1104	60-61	0.35902465081131396
1104	62-63	0.34545027386292304
1104	64-65	0.3503218241254018
1104	66-67	0.3131613714005823
1104	68-69	0.3374784770232182
1104	70-71	0.3702861279472245
1104	72-73	0.37359594402229845
1104	74-75	0.35773174460211976
1104	76-77	0.37049042057627446
1104	78-79	0.38674389254746444
1104	80-81	0.3458799214651904
1104	82-83	0.3583450679382878
1104	84-85	0.36884939278121465
1104	86-87	0.38808557351759987
1104	88-89	0.32682291454909773
1104	90-91	0.3228928505013826
1104	92-93	0.3771998331809172
1104	94-95	0.36330703414081
1104	96-97	0.37733465531648136
1104	98-99	0.3945623112012768
1104	100	0.45077378731205897
1105	1	0.09196270895593273
1105	2	0.1437528527917351
1105	3	0.12938994776054358
1105	4	0.0949634363208105
1105	5	0.066072235736506
1105	6	0.10897828446638869
1105	7	0.12586131459745076
1105	8	0.14903642605502654
1105	9	0.180980252296024
1105	10-11	0.15678471837228614
1105	12-13	0.11037102906137619
1105	14-15	0.2500490528244015
1105	16-17	0.23221751543226787
1105	18-19	0.20148325510646004
1105	20-21	0.18728490452608781
1105	22-23	0.1809458098377661
1105	24-25	0.22441650976125516
1105	26-27	0.21791604971851797
1105	28-29	0.2204952546772816
1105	30-31	0.21112248764846697
1105	32-33	0.21400957151145406
1105	34-35	0.2671807110583728
1105	36-37	0.2589574071027698
1105	38-39	0.2655546802200206
1105	40-41	0.2434865817930998
1105	42-43	0.2712238166482166
1105	44-45	0.3011893441575211
1105	46-47	0.2583001219122991
1105	48-49	0.25554123924485594
1105	50-51	0.2742375115950182
1105	52-53	0.29298603243429966
1105	54-55	0.3053001427194033
1105	56-57	0.3417704498023397
1105	58-59	0.32037275824099254
1105	60-61	0.33847387705499443
1105	62-63	0.30446877185070775
1105	64-65	0.30570640798656257
1105	66-67	0.2921420064026563
1105	68-69	0.2981259169267503
1105	70-71	0.3125457994197518
1105	72-73	0.28939125452443193
1105	74-75	0.26354138536697747
1105	76-77	0.3349604789490783
1105	78-79	0.2917316115414934
1105	80-81	0.2786134798451414
1105	82-83	0.26573060989251474
1105	84-85	0.25268051370689903
1105	86-87	0.2731332493174534
1105	88-89	0.2927163054267581
1105	90-91	0.3117673626444102
1105	92-93	0.30052093697271687
1105	94-95	0.3167488098226485
1105	96-97	0.273168384254614
1105	98-99	0.32340646333202017
1105	100	0.3615941356164072
1106	1	0.0843399888294627
1106	2	0.14094452140366798
1106	3	0.10734253379704484
1106	4	0.10303989284769699
1106	5	0.08266598473035458
1106	6	0.11671673780457326
1106	7	0.07985457698031695
1106	8	0.12772794431261758
1106	9	0.1390793504687977
1106	10-11	0.12614748030249245
1106	12-13	0.135607133980578
1106	14-15	0.1995736344303367
1106	16-17	0.15524723678932162
1106	18-19	0.1388581201113155
1106	20-21	0.12381592469375136
1106	22-23	0.13584679937433464
1106	24-25	0.13320567949600104
1106	26-27	0.17074583047332226
1106	28-29	0.13256902627632172
1106	30-31	0.15974681106715138
1106	32-33	0.16784329634686657
1106	34-35	0.1838606822355331
1106	36-37	0.1853493834647395
1106	38-39	0.18811532584309987
1106	40-41	0.2011711523274684
1106	42-43	0.1936372793852783
1106	44-45	0.2175502216932017
1106	46-47	0.2017802682071732
1106	48-49	0.19914755516822424
1106	50-51	0.19220318096098765
1106	52-53	0.20209693207218038
1106	54-55	0.22748273384784312
1106	56-57	0.25240036473465466
1106	58-59	0.2555852037848325
1106	60-61	0.22511766324901572
1106	62-63	0.19221997738269891
1106	64-65	0.19059261840180852
1106	66-67	0.1968870633140014
1106	68-69	0.20091613812451925
1106	70-71	0.1694829439364156
1106	72-73	0.21564700504571022
1106	74-75	0.20420749190620313
1106	76-77	0.22719850644755724
1106	78-79	0.20931905551947239
1106	80-81	0.21950159141042747
1106	82-83	0.16104618143542382
1106	84-85	0.16860733407180817
1106	86-87	0.1681001520723484
1106	88-89	0.12927902485248666
1106	90-91	0.1738985533472075
1106	92-93	0.20425707005091454
1106	94-95	0.23646707918909726
1106	96-97	0.242030007415174
1106	98-99	0.26763220933790066
1106	100	0.2929134757894154
1107	1	0.04430233378042914
1107	2	0.11154824310226275
1107	3	0.050266159053705906
1107	4	0.09799490801202637
1107	5	0.07550611484711567
1107	6	0.07646212129805718
1107	7	0.01747462747439954
1107	8	0.027202847109570882
1107	9	0.039407580174462
1107	10-11	0.07340765494697621
1107	12-13	0.04137251535974684
1107	14-15	0.15726390999719086
1107	16-17	0.12073741995685339
1107	18-19	0.09007488510965089
1107	20-21	0.07171917128333405
1107	22-23	0.09231825120520654
1107	24-25	0.12408453588594881
1107	26-27	0.132498568254114
1107	28-29	0.1327405026759223
1107	30-31	0.15412932134779567
1107	32-33	0.15877835684199226
1107	34-35	0.16754863171240686
1107	36-37	0.18531677260654078
1107	38-39	0.1353618181057641
1107	40-41	0.1685847522690338
1107	42-43	0.17674615927092674
1107	44-45	0.1540878327317543
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2216	78-79	-0.8366549063688211
2216	80-81	-0.8841416465199003
2216	82-83	-0.9425672293555039
2216	84-85	-0.9452033261106934
2216	86-87	-0.9568315504066831
2216	88-89	-0.9292904475131039
2216	90-91	-0.9830642622583952
2216	92-93	-0.9059340601373123
2216	94-95	-0.9816333917668558
2216	96-97	-1.1588263045695975
2216	98-99	-1.2617005684240858
2216	100	-1.2864760822407817
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	367.0
3	838.0
4	664.0
5	629.0
6	1131.0
7	2514.0
8	5344.0
9	9209.0
10	12510.0
11	16129.0
12	21702.0
13	31752.0
14	37125.0
15	50724.0
16	69575.0
17	106182.0
18	115559.0
19	64392.0
20	44302.0
21	40720.0
22	42130.0
23	43261.0
24	48677.0
25	58597.0
26	72671.0
27	93554.0
28	118680.0
29	150315.0
30	192420.0
31	243026.0
32	307082.0
33	390524.0
34	523815.0
35	777733.0
36	1278791.0
37	2491155.0
38	3609768.0
39	1772965.0
40	13424.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.67481001929876	22.046023572715473	15.812451755039064	23.4667146529467
2	13.591244013587605	22.030907415235323	39.83028402274471	24.54756454843236
3	15.342107094631505	28.79658411959314	31.688822688450603	24.172486097324754
4	10.685128285737742	18.944564173217167	39.279165779886945	31.091141761158152
5	11.368375392690433	40.04012328446053	34.98609495989149	13.60540636295755
6	27.427716504451222	40.62866471927779	18.109760104137223	13.833858672133767
7	24.771212280975146	35.059560079365745	23.02951114296192	17.13971649669719
8	25.777973112816248	35.54910296738185	19.879858064833194	18.79306585496871
9	26.16954521461815	15.962410757859512	21.637803426388082	36.23024060113425
10-11	23.345302210287002	28.267809237454312	28.421843671594836	19.96504488066385
12-13	24.557852297472866	26.29728282118539	28.917260681140743	20.227604200200997
14-15	21.784849030587665	27.17914820237332	27.703201317329544	23.33280144970947
16-17	19.61138514004247	30.425368484931052	28.414692865201097	21.548553509825382
18-19	19.808252065559167	28.13800451572307	32.29022712052825	19.76351629818951
20-21	21.39181113838959	26.92499492222213	32.11407566246727	19.56911827692101
22-23	20.7959381820591	27.14405088166709	32.13886579394206	19.92114514233175
24-25	20.17849457691559	27.39727912839666	32.180987755996135	20.243238538691614
26-27	19.946164668059517	27.590269360175107	32.2016926029918	20.26187336877358
28-29	20.05253222085337	27.377338438498477	32.63339491226888	19.936734428379282
30-31	20.35418858148588	27.421987520616163	32.210147573196956	20.013676324701006
32-33	20.008391174779632	27.563967155522	32.175878432398534	20.251763237299834
34-35	20.41682572251272	27.2008514325213	32.14735540122548	20.234967443740498
36-37	20.305656217862797	27.385994129048406	31.81445002803199	20.4938996250568
38-39	20.185344496092974	27.56827234176502	31.953451314069607	20.2929318480724
40-41	20.315792170783748	27.277259006415992	31.830371478415497	20.57657734438476
42-43	20.239016071966105	27.551900231739086	31.84155006162264	20.367533634672167
44-45	20.37954722157065	27.532344690644123	31.391557411808886	20.69655067597634
46-47	20.563840265955392	27.419728969919753	31.003539607342233	21.012891156782622
48-49	20.53799907495335	27.810995613337962	30.475084860743777	21.17592045096491
50-51	20.723535529116397	27.879351983013983	30.078736722482784	21.31837576538683
52-53	20.810327460649717	28.111244372553163	29.860004982173006	21.218423184624115
54-55	20.735356990166736	28.376112048829295	29.833360057394266	21.055170903609707
56-57	20.825897326925972	28.031065780709714	29.88033437629893	21.26270251606539
58-59	20.937829590643076	27.57591443152268	30.158847427985634	21.32740854984861
60-61	20.804485880843313	27.91029155900409	30.147345426534304	21.137877133618286
62-63	20.58120737094474	27.815888439065922	30.282834019991405	21.320070169997933
64-65	20.780789189772612	27.90600834132852	30.077718027512358	21.23548444138651
66-67	20.61499577525311	27.720688786932318	30.200229518920068	21.4640859188945
68-69	20.912363458898163	27.900928812310923	30.0518341407032	21.134873588087714
70-71	20.870903113697548	27.82703618265876	29.932308590029464	21.369752113614226
72-73	20.731925521362594	27.80395205084683	29.93870274517269	21.525419682617887
74-75	20.748090502528473	27.958029780571085	29.6845868810463	21.609292835854134
76-77	20.87353176013977	27.764099659438955	29.501768901853165	21.860599678568107
78-79	20.861920783490827	27.792862804150815	29.671301429076042	21.673914983282312
80-81	20.636155364761745	28.146126627493906	29.55503113696501	21.66268687077934
82-83	20.646893518323576	28.023625692549896	29.666837703361715	21.662643085764813
84-85	20.624649053797324	27.82914642185147	29.52964868948591	22.016555834865294
86-87	20.808205751567336	27.854291564228195	29.374922798138766	21.962579886065704
88-89	20.57496930782656	27.95538336212037	29.402250676440882	22.06739665361219
90-91	20.505358669398195	27.962884474989046	29.34091801484072	22.190838840772038
92-93	20.50028435232503	27.999479623775454	29.38905599499344	22.111180028906073
94-95	20.49642704842847	27.763835272842304	29.53821925984817	22.20151841888106
96-97	20.713605582622886	27.963142029431786	29.192871175953478	22.130381211991846
98-99	20.35153423411118	27.94694400661654	29.265478296832427	22.436043462439848
100	20.247898956113016	27.73757404499578	29.567080409616953	22.447446589274254
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2590.0
1	1684.5
2	2693.5
3	5238.0
4	3964.0
5	2764.5
6	7349.5
7	23705.0
8	41133.5
9	43054.0
10	29970.0
11	17464.0
12	15412.5
13	16260.5
14	17485.5
15	18842.5
16	20292.5
17	22215.5
18	24176.0
19	26403.5
20	29914.0
21	35503.5
22	45819.5
23	61174.0
24	79228.5
25	98943.5
26	124572.5
27	152965.0
28	187113.0
29	224212.0
30	249760.5
31	271485.5
32	298571.5
33	323940.5
34	342271.5
35	360911.5
36	373308.5
37	385299.0
38	400133.5
39	403774.0
40	415404.5
41	434292.0
42	448570.5
43	475839.5
44	491833.0
45	482864.0
46	475384.5
47	472883.0
48	459624.5
49	440337.5
50	437727.5
51	411975.0
52	354503.0
53	310836.5
54	287055.5
55	269851.5
56	235884.5
57	196785.5
58	174251.5
59	160509.0
60	136072.5
61	107391.5
62	90489.0
63	74698.5
64	57767.5
65	44308.0
66	34463.5
67	25894.5
68	18788.0
69	13610.5
70	9394.0
71	6414.0
72	4500.5
73	3190.0
74	2310.0
75	1569.0
76	947.5
77	552.5
78	354.5
79	239.5
80	119.5
81	41.5
82	27.0
83	18.0
84	7.0
85	6.5
86	7.5
87	7.5
88	8.0
89	8.0
90	4.0
91	3.0
92	5.5
93	3.0
94	4.0
95	4.0
96	1.5
97	2.0
98	0.5
99	1.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.048849311770584596
2	0.0
3	1.6329760381761802E-4
4	0.07115887488262013
5	0.0045878850596378406
6	7.776076372267525E-6
7	0.0
8	0.0
9	0.0
10-11	0.011411392076302594
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.40437307872593E-4
26-27	0.0
28-29	3.032669785184335E-4
30-31	0.0039424707207396355
32-33	1.2830526014241417E-4
34-35	0.0016874085727820532
36-37	0.004210745355582865
38-39	0.001345261212402282
40-41	0.0010692105011867847
42-43	0.0018273779474828687
44-45	1.1664114558401289E-5
46-47	4.276842004747139E-4
48-49	4.976688878251216E-4
50-51	0.0015591033126396388
52-53	8.20376057274224E-4
54-55	0.0010419942338838486
56-57	0.006150876410463612
58-59	0.009346843799465565
60-61	0.0010925387303035875
62-63	0.002558329126476016
64-65	0.0010419942338838486
66-67	1.0886506921174535E-4
68-69	7.153990262486124E-4
70-71	4.0824400954404507E-4
72-73	0.0
74-75	2.177301384234907E-4
76-77	0.0
78-79	5.715416133616631E-4
80-81	0.0
82-83	0.0011080908830481224
84-85	6.143100334091345E-4
86-87	3.888038186133763E-6
88-89	0.0
90-91	3.888038186133763E-6
92-93	2.29394252981892E-4
94-95	0.0
96-97	3.965798949856438E-4
98-99	5.482133842448606E-4
100	3.032669785184335E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2859956E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.1288187232009
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.66512409444954	16.79716946398105
2	17.81172055207411	10.376687582193572
3	7.983869184196972	6.976820345286702
4	4.489387487725095	5.2308221723260235
5	2.889977894547181	4.209082110216132
6	1.8949128688741594	3.3117944072217567
7	1.3387870073529924	2.7298098832313356
8	0.9866631887977912	2.299226653387696
9	0.7611089442022011	1.9953184017845467
>10	3.75580692007836	18.878591784355038
>50	0.21906357185072486	4.426677376909457
>100	0.16984874412541423	10.19644546975308
>500	0.022297227057358843	4.430288881246763
>1k	0.01111103388804934	5.764643479246117
>5k	1.8741378838460057E-4	0.3633839056876348
>10k+	1.3386699170328612E-4	2.0132380831731203
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93499	0.7270553647306414	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	72217	0.5615649073760439	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62737	0.48784770336694777	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14976	0.11645451975107846	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14719	0.11445606812340572	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.776076372267525E-6	0.0
8	0.0	0.0	0.0	7.776076372267525E-6	0.0
9	0.0	0.0	0.0	1.555215274453505E-5	0.0
10-11	0.0	0.0	0.0	3.11043054890701E-5	0.0
12-13	0.0	0.0	0.0	3.11043054890701E-5	0.0
14-15	0.0	0.0	0.0	3.11043054890701E-5	0.0
16-17	0.0	0.0	0.0	5.0544496419738916E-5	0.0
18-19	0.0	0.0	0.0	5.4432534605872676E-5	0.0
20-21	0.0	0.0	0.0	8.942487828107654E-5	0.0
22-23	0.0	0.0	0.0	2.449464057264271E-4	0.0
24-25	0.0	3.888038186133763E-6	0.0	4.782286968944529E-4	0.0
26-27	0.0	7.776076372267525E-6	0.0	9.253530882998356E-4	7.776076372267525E-6
28-29	0.0	7.776076372267525E-6	0.0	0.0024533520954504043	7.776076372267525E-6
30-31	0.0	7.776076372267525E-6	0.0	0.006920707971318097	7.776076372267525E-6
32-33	0.0	1.1664114558401287E-5	0.0	0.014510158510651204	7.776076372267525E-6
34-35	0.0	1.555215274453505E-5	0.0	0.0262325936418445	7.776076372267525E-6
36-37	0.0	1.555215274453505E-5	0.0	0.04679642760830597	7.776076372267525E-6
38-39	0.0	1.555215274453505E-5	0.0	0.08102671579902762	7.776076372267525E-6
40-41	0.0	1.555215274453505E-5	0.0	0.122515971283261	7.776076372267525E-6
42-43	0.0	1.555215274453505E-5	0.0	0.16279215885342063	7.776076372267525E-6
44-45	0.0	1.555215274453505E-5	0.0	0.2121663557791333	7.776076372267525E-6
46-47	0.0	1.555215274453505E-5	0.0	0.2738150892584702	7.776076372267525E-6
48-49	0.0	1.555215274453505E-5	0.0	0.34476012204085305	7.776076372267525E-6
50-51	0.0	1.555215274453505E-5	0.0	0.4230535469950286	7.776076372267525E-6
52-53	0.0	1.555215274453505E-5	0.0	0.5023150934575515	7.776076372267525E-6
54-55	0.0	1.555215274453505E-5	0.0	0.5883651545930639	7.776076372267525E-6
56-57	0.0	1.555215274453505E-5	0.0	0.6905389100864731	7.776076372267525E-6
58-59	0.0	1.555215274453505E-5	0.0	0.8092679321764398	7.776076372267525E-6
60-61	0.0	1.555215274453505E-5	0.0	0.9431369749632115	7.776076372267525E-6
62-63	0.0	1.555215274453505E-5	0.0	1.0824881515924316	7.776076372267525E-6
64-65	0.0	1.555215274453505E-5	0.0	1.2216915827705788	7.776076372267525E-6
66-67	0.0	1.555215274453505E-5	0.0	1.3817232345118442	7.776076372267525E-6
68-69	0.0	1.555215274453505E-5	0.0	1.5735007180428924	7.776076372267525E-6
70-71	0.0	1.555215274453505E-5	0.0	1.7886530871489763	7.776076372267525E-6
72-73	0.0	1.555215274453505E-5	0.0	2.0149524617347057	7.776076372267525E-6
74-75	0.0	1.555215274453505E-5	0.0	2.2373949024397906	7.776076372267525E-6
76-77	0.0	1.555215274453505E-5	0.0	2.4721624241949196	7.776076372267525E-6
78-79	0.0	1.555215274453505E-5	0.0	2.7504448693292574	7.776076372267525E-6
80-81	0.0	1.555215274453505E-5	0.0	3.0656247968500048	7.776076372267525E-6
82-83	0.0	1.555215274453505E-5	0.0	3.3976438177548975	7.776076372267525E-6
84-85	0.0	1.555215274453505E-5	0.0	3.726641832989164	7.776076372267525E-6
86-87	0.0	1.555215274453505E-5	0.0	4.066522467106419	7.776076372267525E-6
88	0.0	1.555215274453505E-5	0.0	4.34230879172526	7.776076372267525E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	33895	0.0	60.282715	1
GTATCAA	67960	0.0	44.583187	1
ATCAACG	93780	0.0	32.02257	3
TATCAAC	95245	0.0	31.628765	2
TCAACGC	95510	0.0	31.44746	4
CAACGCA	99965	0.0	30.066866	5
GTGGTAT	11870	0.0	29.449417	1
AACGCAG	102850	0.0	29.24096	6
TGGTATC	12365	0.0	27.610863	2
ACGCAGA	113495	0.0	26.254015	7
CGCAGAG	114005	0.0	26.04998	8
GCAGAGT	121600	0.0	24.144604	9
GTACATG	79995	0.0	24.119347	1
TACATGG	79010	0.0	23.509996	2
CATGGGT	24815	0.0	22.816536	4
ACATGGG	81100	0.0	22.202509	3
AGTACTT	76320	0.0	22.176884	12-13
AGAGTAC	118595	0.0	22.156294	10-11
GAGTACT	76055	0.0	20.63831	12-13
CAGAGTA	120255	0.0	20.398312	10-11
>>END_MODULE