FastQCFastQC Report
Wed 25 May 2016
SRR2431439_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431439_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10640979
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA185360.1741944984573318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126850.046.4742771
GTATCAA221200.034.706171
ATCAACG241350.030.559093
TCAACGC242650.030.2597624
TATCAAC269700.027.974412
CAACGCA277800.026.4986825
AACGCAG290300.025.4194646
GTACATG446800.024.8000181
TACATGG438800.024.0393682
CGCAGAG305700.023.2155488
ACGCAGA312950.023.0540477
ACATGGG453100.022.3572313
CATGGGT149650.021.7367274
GCAGAGT338350.019.8779169
GTGGTAT70400.019.5659331
TGGTATC67300.018.5794732
AGAGTAC306500.017.33554510-11
CAGAGTA337150.016.93754810-11
CATGGGA166450.016.6339864
CATGGGG200100.016.5617714
Produced by FastQC (version 0.11.5)