FastQCFastQC Report
Wed 25 May 2016
SRR2431439_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431439_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10640979
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT531960.4999164080673404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT445800.4189464146109113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336510.3162396993735257No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107420.10094935813706614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA233200.060.349841
GTATCAA459350.044.0569041
ATCAACG616550.032.45663
TATCAAC625550.032.1550332
TCAACGC624800.032.0506134
GTGGTAT88550.031.0853331
TGGTATC85650.030.9687352
CAACGCA658400.030.4156825
AACGCAG677850.029.5834436
ACGCAGA754600.026.3440367
CGCAGAG751900.026.2698388
GCAGAGT803000.024.1707869
GTACATG431000.022.5440031
AGAGTAC770250.022.16845110-11
TACATGG431300.021.9500772
GAGTACT539900.021.60903212-13
AGTACTT565800.021.58751912-13
CATGGGT144500.021.3179364
ACATGGG434400.021.0464173
CAGAGTA801450.020.38167210-11
Produced by FastQC (version 0.11.5)