##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431439_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10640979 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.280708664118215 33.0 31.0 34.0 30.0 34.0 2 32.41774774670639 34.0 31.0 34.0 31.0 34.0 3 32.522024900152516 34.0 31.0 34.0 31.0 34.0 4 36.057278658288865 37.0 35.0 37.0 35.0 37.0 5 35.96625272918968 37.0 35.0 37.0 35.0 37.0 6 36.003229214154075 37.0 35.0 37.0 35.0 37.0 7 35.94007938555278 37.0 35.0 37.0 35.0 37.0 8 35.92508978732126 37.0 35.0 37.0 35.0 37.0 9 37.61791166019593 39.0 37.0 39.0 35.0 39.0 10-11 37.55315906553335 39.0 37.0 39.0 34.5 39.0 12-13 37.53580539910848 39.0 37.0 39.0 35.0 39.0 14-15 38.87860830286387 40.0 38.0 41.0 35.0 41.0 16-17 38.94215621513773 40.0 38.0 41.0 35.0 41.0 18-19 39.02522324308694 40.5 38.5 41.0 35.0 41.0 20-21 39.05698517025549 41.0 39.0 41.0 35.0 41.0 22-23 39.01193203181775 40.5 39.0 41.0 35.0 41.0 24-25 38.96586037807236 41.0 38.5 41.0 35.0 41.0 26-27 38.84129359713989 40.0 38.0 41.0 35.0 41.0 28-29 38.756895441669414 40.0 38.0 41.0 34.5 41.0 30-31 38.59718006209767 40.0 38.0 41.0 34.0 41.0 32-33 38.42234534999082 40.0 38.0 41.0 34.0 41.0 34-35 38.29317001753316 40.0 38.0 41.0 34.0 41.0 36-37 38.15933684297281 40.0 38.0 41.0 34.0 41.0 38-39 38.05864136185214 40.0 38.0 41.0 33.0 41.0 40-41 37.94134026577818 40.0 38.0 41.0 33.0 41.0 42-43 37.82079101932256 40.0 37.5 41.0 33.0 41.0 44-45 37.60989369493164 40.0 37.0 41.0 33.0 41.0 46-47 37.30349862545542 40.0 37.0 41.0 32.0 41.0 48-49 37.06951413962945 40.0 37.0 41.0 31.0 41.0 50-51 36.944641982659675 40.0 37.0 41.0 31.0 41.0 52-53 36.82104508429158 40.0 37.0 41.0 31.0 41.0 54-55 36.68214621041918 40.0 36.0 41.0 31.0 41.0 56-57 36.474730050684244 40.0 36.0 41.0 30.0 41.0 58-59 36.3246552784288 39.5 35.0 41.0 30.0 41.0 60-61 36.14147128755728 39.0 35.0 41.0 30.0 41.0 62-63 35.943289428538485 39.0 35.0 41.0 29.5 41.0 64-65 35.721902373832336 39.0 35.0 41.0 29.0 41.0 66-67 35.47841627166072 38.0 35.0 41.0 29.0 41.0 68-69 35.206624362288466 37.5 35.0 40.0 29.0 41.0 70-71 34.89221433479006 37.0 35.0 40.0 28.0 41.0 72-73 34.57720187212098 36.5 34.5 39.5 28.0 41.0 74-75 34.266953397802965 36.0 34.0 39.0 28.0 41.0 76-77 33.905340805578135 36.0 34.0 39.0 28.0 40.5 78-79 33.58312336675037 35.0 34.0 37.5 27.0 39.5 80-81 33.260688607692956 35.0 34.0 37.0 27.0 39.0 82-83 32.983372112659936 35.0 34.0 37.0 26.5 39.0 84-85 32.73572154404214 35.0 34.0 36.0 26.5 38.0 86-87 32.473157310055775 35.0 34.0 36.0 26.0 37.0 88-89 32.302259406770744 35.0 34.0 36.0 26.0 37.0 90-91 32.103688955687254 35.0 34.0 35.0 26.0 36.5 92-93 31.901035421646824 35.0 33.5 35.0 26.0 36.0 94-95 31.737465368553025 35.0 33.0 35.0 26.0 36.0 96-97 31.57025345130368 35.0 33.0 35.0 25.0 36.0 98-99 31.40616305135082 35.0 33.0 35.0 25.0 36.0 100 31.298413614010517 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16040566850291782 1101 2 0.23010170709647326 1101 3 0.20485020823344513 1101 4 0.15426656272853023 1101 5 0.1843351680541474 1101 6 0.17842651189251768 1101 7 0.1900652716274891 1101 8 0.2519913983428026 1101 9 0.31990795458489174 1101 10-11 0.29564733444589564 1101 12-13 0.3348766945469137 1101 14-15 0.4313684205042989 1101 16-17 0.4086492642001929 1101 18-19 0.4003614377895772 1101 20-21 0.345806643284412 1101 22-23 0.36699960940428156 1101 24-25 0.32414294089615936 1101 26-27 0.352848705084142 1101 28-29 0.3786014027904372 1101 30-31 0.4247987588230444 1101 32-33 0.43988384848796613 1101 34-35 0.4541847855284047 1101 36-37 0.4748868190611759 1101 38-39 0.42715389659264247 1101 40-41 0.4666585637091174 1101 42-43 0.43994692000447344 1101 44-45 0.5174935318284284 1101 46-47 0.5238543307352117 1101 48-49 0.4925529041707719 1101 50-51 0.3995709540793797 1101 52-53 0.4253893178238144 1101 54-55 0.41450851681604206 1101 56-57 0.43116003141908976 1101 58-59 0.45298948301749675 1101 60-61 0.47552227516976586 1101 62-63 0.4883841048759763 1101 64-65 0.4554056678794609 1101 66-67 0.41979098575169616 1101 68-69 0.40583288951003027 1101 70-71 0.4185880101354442 1101 72-73 0.47907330121901737 1101 74-75 0.39751438347224877 1101 76-77 0.4122636495134273 1101 78-79 0.3829922295602799 1101 80-81 0.42699744871318046 1101 82-83 0.40745820177328085 1101 84-85 0.39388702163014955 1101 86-87 0.40467434140325764 1101 88-89 0.3959201350985637 1101 90-91 0.39741332623435 1101 92-93 0.4042759249138399 1101 94-95 0.44927035609984856 1101 96-97 0.48310672939723887 1101 98-99 0.4998807236765792 1101 100 0.5218636624462363 1102 1 0.19587381456330633 1102 2 0.2533965381637415 1102 3 0.2525856652095868 1102 4 0.198231598306009 1102 5 0.16860370695847848 1102 6 0.16176300652369946 1102 7 0.1803461306949572 1102 8 0.21440096766163919 1102 9 0.29958530550159423 1102 10-11 0.3028822104457447 1102 12-13 0.3178044216580673 1102 14-15 0.4217471627198961 1102 16-17 0.4029017202944516 1102 18-19 0.36278686043168307 1102 20-21 0.36238967606028183 1102 22-23 0.3414440522875921 1102 24-25 0.3375148678219446 1102 26-27 0.35889276701934847 1102 28-29 0.3589837348353626 1102 30-31 0.3159577444726267 1102 32-33 0.3662241451066919 1102 34-35 0.40750380872014347 1102 36-37 0.4097106692034842 1102 38-39 0.43025084446495043 1102 40-41 0.47123188450966325 1102 42-43 0.4577100886833705 1102 44-45 0.4500981274656439 1102 46-47 0.46036013183384483 1102 48-49 0.42984775350331716 1102 50-51 0.37697030889361116 1102 52-53 0.37481537834557344 1102 54-55 0.37282633637290985 1102 56-57 0.4494196175279299 1102 58-59 0.3717946974260826 1102 60-61 0.38668631655420427 1102 62-63 0.4044628755198403 1102 64-65 0.3889130716390241 1102 66-67 0.35111272672290994 1102 68-69 0.3883496127756416 1102 70-71 0.39815271347222136 1102 72-73 0.3446998657687388 1102 74-75 0.39048205299626204 1102 76-77 0.39738274145755526 1102 78-79 0.404152271317507 1102 80-81 0.3626637613144865 1102 82-83 0.3336086331850652 1102 84-85 0.3389778539132351 1102 86-87 0.37019219243259727 1102 88-89 0.33813963079896325 1102 90-91 0.3250683715017928 1102 92-93 0.3567862646994939 1102 94-95 0.39796239838723935 1102 96-97 0.4231826778217176 1102 98-99 0.4878523132099204 1102 100 0.49023600151158675 1103 1 0.14112482044058794 1103 2 0.1725170250376138 1103 3 0.15561751930352585 1103 4 0.12933470225954835 1103 5 0.10241581861404825 1103 6 0.1286404927598852 1103 7 0.14373352359964287 1103 8 0.14710186730356867 1103 9 0.23464342160471574 1103 10-11 0.24953645359298804 1103 12-13 0.2384514553614281 1103 14-15 0.32278875198973367 1103 16-17 0.308039093247956 1103 18-19 0.2898761869373274 1103 20-21 0.2808967422809374 1103 22-23 0.256300014763859 1103 24-25 0.2962435159924226 1103 26-27 0.2947424759572499 1103 28-29 0.2701142675982098 1103 30-31 0.27107864362922385 1103 32-33 0.3201152509325169 1103 34-35 0.26719113930710137 1103 36-37 0.2735473702212019 1103 38-39 0.2763906098090345 1103 40-41 0.29036237824574584 1103 42-43 0.3559964635649493 1103 44-45 0.36720165621049006 1103 46-47 0.34869168019313435 1103 48-49 0.30628099259702424 1103 50-51 0.2782212667379511 1103 52-53 0.29316916445439745 1103 54-55 0.3037051865444056 1103 56-57 0.3779669438569542 1103 58-59 0.3742478796958224 1103 60-61 0.3651336117869093 1103 62-63 0.3722374019013017 1103 64-65 0.3523050677003212 1103 66-67 0.3525661484068294 1103 68-69 0.3247965562671382 1103 70-71 0.36415274799589525 1103 72-73 0.34457923304484694 1103 74-75 0.3239683912527198 1103 76-77 0.3352988674409474 1103 78-79 0.3984659969548616 1103 80-81 0.36264820064375414 1103 82-83 0.28576947748403825 1103 84-85 0.2847804380352983 1103 86-87 0.29456047798441176 1103 88-89 0.2748101776264491 1103 90-91 0.26034408682357224 1103 92-93 0.25823600886155873 1103 94-95 0.32799952134430654 1103 96-97 0.34038740096061204 1103 98-99 0.38594820746940783 1103 100 0.37898886378689056 1104 1 0.12341539165452531 1104 2 0.17066763309109945 1104 3 0.1348593154314699 1104 4 0.12383807867708896 1104 5 0.10890130901243111 1104 6 0.12260844664098158 1104 7 0.13954877469404892 1104 8 0.13068496746036118 1104 9 0.2043104715290056 1104 10-11 0.2009096485297377 1104 12-13 0.2130105583156734 1104 14-15 0.3081844815252168 1104 16-17 0.2578513104529705 1104 18-19 0.2570130893521636 1104 20-21 0.2485577513088586 1104 22-23 0.22754627314485987 1104 24-25 0.2294973559460658 1104 26-27 0.24174032745050056 1104 28-29 0.2613251227186666 1104 30-31 0.2590690668232014 1104 32-33 0.2103614425700684 1104 34-35 0.21648072057630685 1104 36-37 0.23432570997808 1104 38-39 0.18424449820512478 1104 40-41 0.2526870129637615 1104 42-43 0.26392229731872874 1104 44-45 0.2754641004989864 1104 46-47 0.3381640987667751 1104 48-49 0.31927532748706966 1104 50-51 0.251049705478394 1104 52-53 0.24158374293171647 1104 54-55 0.2798325682484304 1104 56-57 0.27612787763595037 1104 58-59 0.3318289299641535 1104 60-61 0.30076663008196647 1104 62-63 0.26049665399094835 1104 64-65 0.23199364903815223 1104 66-67 0.2493936121187872 1104 68-69 0.2536931787655732 1104 70-71 0.2458413631462193 1104 72-73 0.2144674414101928 1104 74-75 0.22297109139001492 1104 76-77 0.2299918700187007 1104 78-79 0.2558307708197347 1104 80-81 0.26566247852860414 1104 82-83 0.27311720379907456 1104 84-85 0.27398371393968546 1104 86-87 0.24913304186882357 1104 88-89 0.20742649207376473 1104 90-91 0.2542743202328026 1104 92-93 0.28408216914095874 1104 94-95 0.22911110505050303 1104 96-97 0.23101411834181462 1104 98-99 0.26481622289464113 1104 100 0.30927478099240346 1105 1 0.0899489065285266 1105 2 0.15607169479110183 1105 3 0.12650446501685053 1105 4 0.13602272242000168 1105 5 0.10448845379364968 1105 6 0.15833406371006475 1105 7 0.1425492272783302 1105 8 0.19403212926513902 1105 9 0.24301564195278047 1105 10-11 0.20454767336623547 1105 12-13 0.15923052898070011 1105 14-15 0.26687727463691147 1105 16-17 0.26450621322339174 1105 18-19 0.2502431647757035 1105 20-21 0.21665637007129845 1105 22-23 0.22304625331023686 1105 24-25 0.22721182430908016 1105 26-27 0.27591760183862135 1105 28-29 0.2890637170813477 1105 30-31 0.2632724988340982 1105 32-33 0.2717563577837083 1105 34-35 0.292951128395174 1105 36-37 0.3118620228647657 1105 38-39 0.31021588463770655 1105 40-41 0.2888175098318584 1105 42-43 0.3279646785229602 1105 44-45 0.3598310014930277 1105 46-47 0.35360498569509957 1105 48-49 0.386309327580598 1105 50-51 0.391862250795441 1105 52-53 0.3570581902966481 1105 54-55 0.3618021349651954 1105 56-57 0.39709595808670883 1105 58-59 0.38085796493810875 1105 60-61 0.38459087351782273 1105 62-63 0.3962864832775779 1105 64-65 0.3992251969131644 1105 66-67 0.4137719588946709 1105 68-69 0.39796026280848196 1105 70-71 0.41860120717129945 1105 72-73 0.3999643646355011 1105 74-75 0.41461995442249844 1105 76-77 0.42060097419965103 1105 78-79 0.4064367470868717 1105 80-81 0.36371899592609935 1105 82-83 0.3678237229772634 1105 84-85 0.3688212810463014 1105 86-87 0.3486980742765766 1105 88-89 0.38853696611112554 1105 90-91 0.39737464680069223 1105 92-93 0.4280005820466215 1105 94-95 0.4501686884481195 1105 96-97 0.42273978509251364 1105 98-99 0.4971595993914093 1105 100 0.5333631392343499 1106 1 0.09703380650493187 1106 2 0.13290776636036838 1106 3 0.12397616423354663 1106 4 0.12781720243999928 1106 5 0.08382221509804566 1106 6 0.10578089042261496 1106 7 0.09159111236159134 1106 8 0.09256394357863229 1106 9 0.15674699248094726 1106 10-11 0.13617746364541716 1106 12-13 0.15911055898922655 1106 14-15 0.20059988983723542 1106 16-17 0.19093012786640884 1106 18-19 0.16582948605127257 1106 20-21 0.1515595635561624 1106 22-23 0.16578623886245936 1106 24-25 0.16078502159093233 1106 26-27 0.19959041428559487 1106 28-29 0.16925822711232996 1106 30-31 0.18988771403340365 1106 32-33 0.21039150370167903 1106 34-35 0.19153737308147356 1106 36-37 0.19558860315756021 1106 38-39 0.22102273132799155 1106 40-41 0.23789185750428032 1106 42-43 0.2787294892735872 1106 44-45 0.2295994698899122 1106 46-47 0.21093512648680957 1106 48-49 0.21485222709664953 1106 50-51 0.2519800848360845 1106 52-53 0.21476648315962876 1106 54-55 0.1857400095798738 1106 56-57 0.20364108146581117 1106 58-59 0.1934631223448875 1106 60-61 0.20415641322895794 1106 62-63 0.17556645125434045 1106 64-65 0.15433459411245565 1106 66-67 0.18491349391338474 1106 68-69 0.22456965007192053 1106 70-71 0.22226920232375136 1106 72-73 0.24750545142921965 1106 74-75 0.21917176550185502 1106 76-77 0.2557929854723042 1106 78-79 0.20627937226866067 1106 80-81 0.1896671467370581 1106 82-83 0.1669519821389116 1106 84-85 0.17583668350401638 1106 86-87 0.1677434727667233 1106 88-89 0.16234497906673795 1106 90-91 0.2349614218579248 1106 92-93 0.24345117365506042 1106 94-95 0.24838834035061197 1106 96-97 0.25245284617698616 1106 98-99 0.22549865883612696 1106 100 0.2722300236923765 1107 1 0.07331787530993239 1107 2 0.12178498768791712 1107 3 0.06896512996135584 1107 4 0.10792547959941601 1107 5 0.08096136404999044 1107 6 0.09583429858006554 1107 7 -0.008410617564926781 1107 8 0.008062550275276692 1107 9 0.06794298613354499 1107 10-11 0.052935035708117084 1107 12-13 0.0407815493045689 1107 14-15 0.1571242392256451 1107 16-17 0.16169132666073693 1107 18-19 0.08619726866728428 1107 20-21 0.09517377507205538 1107 22-23 0.12371816170834649 1107 24-25 0.12201139961553054 1107 26-27 0.12223643170656828 1107 28-29 0.12036120334064293 1107 30-31 0.15140858955741265 1107 32-33 0.1425984420492341 1107 34-35 0.11304410492372341 1107 36-37 0.19273411096272497 1107 38-39 0.13517707844465576 1107 40-41 0.1476392148325516 1107 42-43 0.11552861985535401 1107 44-45 0.13020029852550152 1107 46-47 0.15480058534897978 1107 48-49 0.17585018993900547 1107 50-51 0.13488809799581958 1107 52-53 0.1755199177444311 1107 54-55 0.1476978149446211 1107 56-57 0.16173877832142836 1107 58-59 0.14425465607337884 1107 60-61 0.13445931189797022 1107 62-63 0.14348578002820034 1107 64-65 0.17494033556421584 1107 66-67 0.15506996875208046 1107 68-69 0.128465672269499 1107 70-71 0.13905227945813436 1107 72-73 0.19271756016331665 1107 74-75 0.21627313550175842 1107 76-77 0.1929876749370436 1107 78-79 0.17483146166961205 1107 80-81 0.189163157365698 1107 82-83 0.20215481646070543 1107 84-85 0.1690202058114494 1107 86-87 0.13110074131449068 1107 88-89 0.13454044656054975 1107 90-91 0.17247326385374606 1107 92-93 0.20755480040898533 1107 94-95 0.23020545532167347 1107 96-97 0.24430535601774395 1107 98-99 0.23639321737098484 1107 100 0.27819237902657434 1108 1 0.005943873528998722 1108 2 0.08505154933418169 1108 3 0.07317644343010699 1108 4 0.06907276260204753 1108 5 0.03750320196571977 1108 6 0.0339291901825689 1108 7 0.039410660977360124 1108 8 0.05204842520120678 1108 9 0.06537827453334444 1108 10-11 0.03692731490109935 1108 12-13 0.04548390228373478 1108 14-15 0.10389165433647207 1108 16-17 0.0959824092112882 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-0.23179733599252472 2215 3 -0.27457925312202747 2215 4 -0.18842735116814424 2215 5 -0.15325856010706218 2215 6 -0.2509702095031088 2215 7 -0.2963231155889616 2215 8 -0.27422891985752074 2215 9 -0.33103138330083226 2215 10-11 -0.3249159270316113 2215 12-13 -0.32106589983458633 2215 14-15 -0.4258504198040356 2215 16-17 -0.43072758134186273 2215 18-19 -0.4065169288116408 2215 20-21 -0.4564887633210972 2215 22-23 -0.4051759018640908 2215 24-25 -0.4532947204702751 2215 26-27 -0.3908406129680202 2215 28-29 -0.41935730810973837 2215 30-31 -0.5070530803897739 2215 32-33 -0.49654987154147534 2215 34-35 -0.47260638923677334 2215 36-37 -0.4545855761213673 2215 38-39 -0.49858960674931296 2215 40-41 -0.5178476112368884 2215 42-43 -0.4781323556155286 2215 44-45 -0.45150793877181883 2215 46-47 -0.471476433276905 2215 48-49 -0.5443328606405302 2215 50-51 -0.49537815971043386 2215 52-53 -0.5157373582579581 2215 54-55 -0.49606282975445026 2215 56-57 -0.48156038361567965 2215 58-59 -0.5085558153784362 2215 60-61 -0.5322796208020293 2215 62-63 -0.4859153037338899 2215 64-65 -0.4677301304738819 2215 66-67 -0.5145109764292002 2215 68-69 -0.6043880228221283 2215 70-71 -0.46108472589268956 2215 72-73 -0.5099107668894334 2215 74-75 -0.5457215918664673 2215 76-77 -0.5383874746411621 2215 78-79 -0.5037897225988388 2215 80-81 -0.5170861999154468 2215 82-83 -0.525470227945334 2215 84-85 -0.4827513982533418 2215 86-87 -0.4669718616947449 2215 88-89 -0.47202738588126536 2215 90-91 -0.465074095496 2215 92-93 -0.4336249828216019 2215 94-95 -0.5413121876827418 2215 96-97 -0.5620359366184182 2215 98-99 -0.5873958206286822 2215 100 -0.567863281913624 2216 1 -0.22715317169735272 2216 2 -0.2778573100476791 2216 3 -0.28958021719915195 2216 4 -0.1525248448054981 2216 5 -0.2549124975196264 2216 6 -0.3270088687975914 2216 7 -0.31664946593789267 2216 8 -0.3716858839568502 2216 9 -0.7434383852099558 2216 10-11 -0.5769170974040563 2216 12-13 -0.5193111060122959 2216 14-15 -0.7014668295068134 2216 16-17 -0.5167501133242425 2216 18-19 -0.5404743220211046 2216 20-21 -0.7639973432110452 2216 22-23 -0.6877187084743142 2216 24-25 -0.5773210290050841 2216 26-27 -0.6955284412927796 2216 28-29 -0.6527038091242972 2216 30-31 -0.6754032642582501 2216 32-33 -0.6655661953693652 2216 34-35 -0.7457333043406393 2216 36-37 -0.7286994808755693 2216 38-39 -0.7880968546401732 2216 40-41 -0.9793895323366542 2216 42-43 -1.0041673525402572 2216 44-45 -0.9963097558358385 2216 46-47 -1.0525799913492406 2216 48-49 -0.7725831171550865 2216 50-51 -0.8901731195363638 2216 52-53 -0.9217304967908859 2216 54-55 -0.8817945147339543 2216 56-57 -1.168080612980141 2216 58-59 -1.2090898126761118 2216 60-61 -1.0064437580052044 2216 62-63 -1.1646431765449563 2216 64-65 -1.1702943063590823 2216 66-67 -1.0671309121829466 2216 68-69 -0.9295551554043442 2216 70-71 -1.009946231092627 2216 72-73 -0.9091846380733983 2216 74-75 -0.88881831232586 2216 76-77 -0.830652179691711 2216 78-79 -0.8046876094527917 2216 80-81 -0.8692721308121989 2216 82-83 -0.8901290553235839 2216 84-85 -0.8766961002035103 2216 86-87 -0.9157725933625471 2216 88-89 -0.9000209180404717 2216 90-91 -0.9054623869742429 2216 92-93 -0.8662013256376468 2216 94-95 -0.9212461117264148 2216 96-97 -1.1401204597511168 2216 98-99 -1.1552744244203375 2216 100 -1.179962621636431 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 279.0 3 776.0 4 440.0 5 490.0 6 838.0 7 1870.0 8 3734.0 9 6258.0 10 9434.0 11 13155.0 12 18015.0 13 25932.0 14 31863.0 15 43654.0 16 57075.0 17 82330.0 18 90272.0 19 50296.0 20 32964.0 21 30160.0 22 29818.0 23 31976.0 24 36856.0 25 45149.0 26 58173.0 27 75621.0 28 95996.0 29 124086.0 30 157613.0 31 198780.0 32 250843.0 33 318310.0 34 419366.0 35 605701.0 36 971822.0 37 1873780.0 38 3078948.0 39 1755454.0 40 12852.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.89406899617457 22.14908851822263 15.746588236864822 24.210254248737982 2 13.085450126346457 21.822794688345876 40.45377779619714 24.63797738911053 3 15.437707012474903 27.48007347078014 32.06365212177655 25.018567394968404 4 10.634383082114685 18.808826838895772 39.45654239792549 31.100247681064054 5 11.484693609993103 39.07271660674756 35.71920494399771 13.723384839261618 6 28.085529356418178 40.335587574751116 17.96930695655982 13.609576112270883 7 24.55029748672561 34.65674539908405 23.077369102974455 17.715588011215885 8 24.650955518284547 36.30984517495994 20.488284019731644 18.550915287023873 9 24.701458390247737 16.5663704439225 22.42619781506946 36.305973350760304 10-11 22.773361476854387 28.054482300287347 29.221229748934867 19.950926473923396 12-13 23.932854298462576 25.88268898942475 29.673143796261602 20.51131291585107 14-15 21.117511837961526 27.42717093981672 28.024587775241354 23.430729446980394 16-17 19.31104741396445 30.741696793124017 28.791984271372023 21.15527152153951 18-19 19.2362751585169 28.629344160908506 32.44285605675944 19.691524623815155 20-21 20.69120237902922 27.60783100878218 32.23817094272999 19.46279566945861 22-23 20.217246928125693 27.650890956555784 32.34242826717354 19.789433848144988 24-25 19.94321353270778 27.734676588682618 32.33014114246091 19.99196873614869 26-27 19.730073708443555 27.81032177584412 32.396384768732275 20.06321974698005 28-29 19.688007033209924 27.807267117723093 32.58412669175529 19.920599157311685 30-31 20.076303103319603 28.037210720327106 32.161319633653754 19.725166542699537 32-33 19.69013333754348 27.891923137790897 32.485421433621994 19.93252209104363 34-35 19.950444517778525 27.77284002270318 32.25558077139027 20.021134688128022 36-37 19.98174251256935 28.01639029352386 31.94416537616514 20.05770181774165 38-39 19.978732857007596 28.070603531539252 31.920045691935144 20.030617919518008 40-41 19.98647383001402 27.848831494163907 31.99738949524164 20.16730518058043 42-43 19.98284335976185 27.966535905551265 31.873637435157466 20.176983299529414 44-45 20.2268532084175 28.012392281987612 31.49900319312755 20.26175131646734 46-47 20.311879826618465 28.212832614982595 30.98882705268447 20.486460505714472 48-49 20.335700873556043 28.308832737058783 30.603359301767895 20.752107087617283 50-51 20.42978456687476 28.370427672417524 30.32788968932327 20.871898071384447 52-53 20.49967397020092 28.565482510710915 30.148431544573047 20.786411974515122 54-55 20.59274482187498 28.75893406826078 29.987423959452936 20.660897150411305 56-57 20.495940142494554 28.491321679535233 30.0507742400816 20.96196393788862 58-59 20.470594814213662 28.22736182036824 30.38885643810717 20.913186927310928 60-61 20.43709343041822 28.51804741346094 30.141849436981598 20.90300971913924 62-63 20.3447370102775 28.300862159703478 30.355925162958897 20.998475667060124 64-65 20.38836589216058 28.2547687018939 30.34932067333796 21.00754473260756 66-67 20.277244526872202 28.302736569771497 30.23161192814469 21.188406975211606 68-69 20.628058303676557 28.38153270205256 30.10297900357554 20.887429990695342 70-71 20.545123653743673 28.32680673475994 29.996865409538167 21.13120420195822 72-73 20.48028193646468 28.28820073792083 30.134501722068997 21.0970156035455 74-75 20.556790459800066 28.319752623614185 29.869299275976367 21.254157640609378 76-77 20.60786418242156 28.21792994798693 29.73840564857801 21.435800221013498 78-79 20.610584365770595 28.28761720907429 29.93016299998844 21.171635425166674 80-81 20.335126119504608 28.295897398162328 30.007896829793573 21.361079652539487 82-83 20.353930155904273 28.310897717679023 30.094208111912796 21.24096401450391 84-85 20.367804888179947 28.136780737856764 29.93508499058845 21.56032938337484 86-87 20.480197357780707 28.26917523284277 29.797469762885537 21.453157646490986 88-89 20.306806356821117 28.142321303331208 29.8490674589246 21.70180488092308 90-91 20.175751686005583 28.163541155376777 29.77432809518748 21.88637906343016 92-93 20.21591492226188 28.224997381577893 29.790740968111823 21.768346728048403 94-95 20.035731674688957 28.021096555119602 29.97310679778618 21.970064972405265 96-97 20.054187922636853 28.190448919323984 29.759428027544953 21.99593513049421 98-99 20.015659369846396 28.08884312422989 29.809315713354874 22.086181792568844 100 20.051284947691176 28.102707853132138 29.68649665222214 22.15951054695455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2276.0 1 1414.0 2 1503.0 3 2728.5 4 2015.5 5 1496.5 6 4195.0 7 12680.5 8 21574.0 9 23105.5 10 16707.5 11 10244.5 12 9378.0 13 10148.0 14 11227.0 15 12452.0 16 13702.0 17 15410.5 18 17599.5 19 20127.0 20 24147.5 21 29992.5 22 38629.5 23 51822.5 24 69100.5 25 89928.0 26 115242.0 27 145423.0 28 179677.5 29 213166.5 30 241545.5 31 268704.0 32 295664.0 33 320043.5 34 340264.5 35 356636.5 36 369076.5 37 376131.5 38 380520.0 39 383365.5 40 383427.0 41 379036.5 42 368562.5 43 359395.5 44 351101.0 45 340438.0 46 329921.5 47 318203.0 48 307256.0 49 302195.0 50 298329.5 51 283639.0 52 263404.0 53 245166.0 54 232026.0 55 219073.0 56 193574.0 57 165834.5 58 147753.0 59 135574.0 60 114820.5 61 89155.5 62 72481.0 63 58791.0 64 46200.5 65 35653.5 66 27173.5 67 20575.5 68 15289.5 69 11431.0 70 8251.0 71 5777.5 72 4392.0 73 3196.5 74 2150.5 75 1430.5 76 922.5 77 594.5 78 375.0 79 217.5 80 122.5 81 61.5 82 26.0 83 12.0 84 8.0 85 7.0 86 7.0 87 3.5 88 1.5 89 3.5 90 3.5 91 0.5 92 1.0 93 1.0 94 0.5 95 2.0 96 2.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04751442512949231 2 0.0 3 1.3156684173514486E-4 4 0.07091452769524308 5 0.00489616603885789 6 9.397631552510348E-6 7 0.0 8 0.0 9 0.0 10-11 0.011559086809587726 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.96749603584407E-4 26-27 0.0 28-29 3.1951947278535176E-4 30-31 0.00366037748970278 32-33 1.409644732876552E-4 34-35 0.0016774772321230967 36-37 0.0038812218311867737 38-39 0.0014237411802053175 40-41 0.0011747039440637933 42-43 0.00188892394205458 44-45 9.397631552510348E-6 46-47 5.027732880593036E-4 48-49 4.463874987442415E-4 50-51 0.0014989222326254003 52-53 7.56509339977083E-4 54-55 0.0010337394707761382 56-57 0.006037978272487898 58-59 0.008777387870044664 60-61 0.0011183181547487313 62-63 0.0024339865721001797 64-65 0.0010478359181049037 66-67 1.0807276285386899E-4 68-69 6.76629471780745E-4 70-71 3.336159201141173E-4 72-73 0.0 74-75 2.631336834702897E-4 76-77 0.0 78-79 5.450626300456001E-4 80-81 0.0 82-83 0.0011512098651825177 84-85 6.155448666894278E-4 86-87 0.0 88-89 0.0 90-91 0.0 92-93 2.678324992465449E-4 94-95 0.0 96-97 4.416886829679863E-4 98-99 6.155448666894278E-4 100 3.7590526210041386E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0640979E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.00561925729644 #Duplication Level Percentage of deduplicated Percentage of total 1 62.99952520456523 28.983321699516786 2 18.399913579345103 16.929988369970186 3 7.788375414764058 10.749271018935703 4 3.801262935995079 6.995178213210496 5 2.1097677113792455 4.853058502552562 6 1.3087856586843574 3.6126896821705485 7 0.860076869756899 2.7697858301430265 8 0.5962211196576661 2.1943617459303653 9 0.41661190905668294 1.7249839979505448 >10 1.619342463118675 12.06450094148209 >50 0.06193377814708818 1.9424535581308622 >100 0.032732696222491066 2.894224207830049 >500 0.003259989370112931 1.0639850933461097 >1k 0.002088302612383206 1.8034969006574868 >5k 2.0473465018500816E-5 0.07961833671892792 >10k+ 8.189386007400327E-5 1.3390819014543076 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53196 0.4999164080673404 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44580 0.4189464146109113 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33651 0.3162396993735257 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10742 0.10094935813706614 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 9.397631552510346E-6 0.0 4 0.0 0.0 0.0 1.8795263105020693E-5 0.0 5 0.0 0.0 0.0 1.8795263105020693E-5 0.0 6 0.0 0.0 0.0 1.8795263105020693E-5 0.0 7 0.0 0.0 0.0 1.8795263105020693E-5 0.0 8 0.0 0.0 0.0 1.8795263105020693E-5 0.0 9 0.0 0.0 0.0 4.698815776255173E-5 0.0 10-11 0.0 0.0 0.0 4.698815776255173E-5 0.0 12-13 0.0 0.0 0.0 6.578342086757243E-5 0.0 14-15 0.0 0.0 0.0 8.457868397259312E-5 0.0 16-17 0.0 0.0 0.0 1.0807276285386899E-4 0.0 18-19 0.0 0.0 0.0 1.1277157863012417E-4 0.0 20-21 0.0 0.0 0.0 1.409644732876552E-4 0.0 22-23 0.0 0.0 0.0 2.7723013079905524E-4 0.0 24-25 0.0 0.0 0.0 4.7927920917802775E-4 0.0 26-27 0.0 0.0 0.0 9.632572341323106E-4 0.0 28-29 0.0 0.0 0.0 0.0025514569665065594 0.0 30-31 0.0 0.0 0.0 0.007071717743264036 0.0 32-33 0.0 0.0 0.0 0.016582120874404506 0.0 34-35 0.0 0.0 0.0 0.031134353333466782 0.0 36-37 0.0 0.0 0.0 0.05572795510638636 0.0 38-39 0.0 0.0 0.0 0.09134967750617683 0.0 40-41 0.0 0.0 0.0 0.130354547264871 0.0 42-43 0.0 0.0 0.0 0.17024749320527743 0.0 44-45 0.0 0.0 0.0 0.2210698846412534 0.0 46-47 0.0 0.0 0.0 0.28351714630768465 0.0 48-49 0.0 0.0 0.0 0.3520634708516951 0.0 50-51 0.0 0.0 0.0 0.4251347549882393 0.0 52-53 0.0 0.0 0.0 0.49799929123062825 0.0 54-55 0.0 0.0 0.0 0.5800547111313724 0.0 56-57 0.0 0.0 0.0 0.6809523822948997 0.0 58-59 0.0 0.0 0.0 0.7929815480323756 0.0 60-61 0.0 0.0 0.0 0.9133229188780468 0.0 62-63 0.0 0.0 0.0 1.0379261156327817 0.0 64-65 0.0 0.0 0.0 1.1679235528986571 0.0 66-67 0.0 0.0 0.0 1.3203766307592564 0.0 68-69 0.0 4.698815776255173E-6 0.0 1.496135834870081 0.0 70-71 0.0 9.397631552510346E-6 0.0 1.6902392157714061 0.0 72-73 0.0 9.397631552510346E-6 0.0 1.8945202316440997 0.0 74-75 4.698815776255173E-6 9.397631552510346E-6 0.0 2.102015237507752 0.0 76-77 9.397631552510346E-6 9.397631552510346E-6 0.0 2.3302743102866756 0.0 78-79 9.397631552510346E-6 9.397631552510346E-6 0.0 2.593619440466897 0.0 80-81 9.397631552510346E-6 9.397631552510346E-6 0.0 2.884758065963667 0.0 82-83 9.397631552510346E-6 9.397631552510346E-6 0.0 3.188198191162674 0.0 84-85 9.397631552510346E-6 9.397631552510346E-6 0.0 3.4925404889907217 0.0 86-87 9.397631552510346E-6 9.397631552510346E-6 0.0 3.818694689652146 0.0 88 9.397631552510346E-6 9.397631552510346E-6 0.0 4.084539589825335 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 23320 0.0 60.34984 1 GTATCAA 45935 0.0 44.056904 1 ATCAACG 61655 0.0 32.4566 3 TATCAAC 62555 0.0 32.155033 2 TCAACGC 62480 0.0 32.050613 4 GTGGTAT 8855 0.0 31.085333 1 TGGTATC 8565 0.0 30.968735 2 CAACGCA 65840 0.0 30.415682 5 AACGCAG 67785 0.0 29.583443 6 ACGCAGA 75460 0.0 26.344036 7 CGCAGAG 75190 0.0 26.269838 8 GCAGAGT 80300 0.0 24.170786 9 GTACATG 43100 0.0 22.544003 1 AGAGTAC 77025 0.0 22.168451 10-11 TACATGG 43130 0.0 21.950077 2 GAGTACT 53990 0.0 21.609032 12-13 AGTACTT 56580 0.0 21.587519 12-13 CATGGGT 14450 0.0 21.317936 4 ACATGGG 43440 0.0 21.046417 3 CAGAGTA 80145 0.0 20.381672 10-11 >>END_MODULE