FastQCFastQC Report
Wed 25 May 2016
SRR2431438_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431438_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4134399
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA496531.2009726202042907No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68900.16665058210395273No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG47400.041.4523162
GGTATCA71050.039.567671
GTACCTG56100.036.4527051
TCTGTCG13550.030.5243878
GTATCAA107100.030.199681
ACCTGGG65650.029.2846093
GTCTCGC12000.028.9903761
ATCAACG117600.026.0610033
TCAACGC116600.026.0426274
TATCAAC125800.025.1469482
CTGTCGC16250.024.5849389
GTACATG181450.024.4319481
CAACGCA126200.024.0612815
TCGCTCT14800.023.8204754
TACATGG182350.023.2257562
CATGGGT86450.022.6736724
AACGCAG135900.022.5495016
CGCTCTG13000.022.0562695
TCTCGCT16050.021.9652962
TATAACG2859.640644E-1121.4412122
Produced by FastQC (version 0.11.5)