##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431438_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4134399
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.9527650814544	31.0	30.0	34.0	25.0	34.0
2	29.87935537910105	31.0	30.0	34.0	25.0	34.0
3	30.000172697410193	31.0	31.0	34.0	25.0	34.0
4	33.233340565339724	37.0	35.0	37.0	25.0	37.0
5	33.353477978298656	37.0	35.0	37.0	27.0	37.0
6	33.357407690936455	37.0	35.0	37.0	27.0	37.0
7	33.19105195217007	37.0	35.0	37.0	26.0	37.0
8	33.10695968144342	37.0	35.0	37.0	25.0	37.0
9	34.390857776426515	39.0	35.0	39.0	22.0	39.0
10-11	34.35708394376063	39.0	35.0	39.0	22.5	39.0
12-13	34.33962348578355	39.0	35.0	39.0	22.5	39.0
14-15	35.52279908155938	40.0	36.0	41.0	23.0	41.0
16-17	35.54209475186116	40.0	36.0	41.0	23.5	41.0
18-19	35.52871953094029	40.0	36.0	41.0	24.0	41.0
20-21	35.62948472075385	40.0	36.0	41.0	25.0	41.0
22-23	35.60761455292534	40.0	36.0	41.0	25.0	41.0
24-25	35.659979721357324	40.0	36.0	41.0	25.5	41.0
26-27	35.6699555364637	40.0	36.0	41.0	26.0	41.0
28-29	35.569792126981454	40.0	35.0	41.0	25.5	41.0
30-31	35.55579976678593	40.0	35.0	41.0	26.0	41.0
32-33	35.490587507398295	40.0	35.0	41.0	26.0	41.0
34-35	35.39092175186768	40.0	35.0	41.0	25.0	41.0
36-37	35.26316993110728	40.0	35.0	41.0	25.0	41.0
38-39	35.14993872144416	40.0	35.0	41.0	25.0	41.0
40-41	35.058133842427885	40.0	35.0	41.0	24.5	41.0
42-43	34.84466327512173	39.5	34.5	41.0	24.0	41.0
44-45	34.72071382079959	39.0	34.0	41.0	24.0	41.0
46-47	34.58453308449427	39.0	34.0	41.0	23.0	41.0
48-49	34.45820420331952	39.0	34.0	41.0	23.0	41.0
50-51	34.3038286822341	39.0	33.0	41.0	22.0	41.0
52-53	34.209590680531804	39.0	33.0	41.0	22.0	41.0
54-55	34.136110230289816	39.0	33.0	41.0	22.0	41.0
56-57	33.985034100482316	38.5	33.0	41.0	20.5	41.0
58-59	33.777415532463124	38.0	33.0	40.0	19.0	41.0
60-61	33.554385655569284	38.0	33.0	40.0	18.0	41.0
62-63	33.256699220370365	37.0	32.5	40.0	15.0	41.0
64-65	33.087501472402636	37.0	32.5	40.0	13.0	41.0
66-67	32.81275803327158	36.5	32.0	40.0	8.5	41.0
68-69	32.52130660345071	36.0	32.0	39.5	7.5	41.0
70-71	32.19843621769452	35.5	32.0	39.0	4.0	41.0
72-73	31.866777734804984	35.0	31.0	39.0	2.0	41.0
74-75	31.52563202051858	35.0	31.0	38.0	2.0	40.0
76-77	31.198893116024845	35.0	31.0	37.0	2.0	39.5
78-79	30.85607206754839	35.0	31.0	37.0	2.0	39.0
80-81	30.555665890012065	35.0	31.0	36.0	2.0	39.0
82-83	30.25921494272807	35.0	30.5	36.0	2.0	38.0
84-85	29.965488454307383	35.0	30.0	36.0	2.0	37.0
86-87	29.708042934414408	35.0	30.0	35.0	2.0	37.0
88-89	29.43912791194077	35.0	30.0	35.0	2.0	36.5
90-91	29.212584223245024	34.5	30.0	35.0	2.0	36.0
92-93	29.010821282609633	34.0	30.0	35.0	2.0	36.0
94-95	28.777050424983173	34.0	29.0	35.0	2.0	36.0
96-97	28.670946369714194	34.0	29.0	35.0	2.0	35.0
98-99	28.503430728383982	34.0	29.0	35.0	2.0	35.0
100	28.360697165416305	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.35536090289044253
1101	2	0.47885041044694887
1101	3	0.5145696716397836
1101	4	0.4844582286557255
1101	5	0.3597167681684468
1101	6	0.5346975738055875
1101	7	0.46315043263036415
1101	8	0.482164213332986
1101	9	0.6005920642483531
1101	10-11	0.653524925413727
1101	12-13	0.5790976209078735
1101	14-15	0.7459791766948598
1101	16-17	0.6725163513400858
1101	18-19	0.6437501369420247
1101	20-21	0.5910185049041203
1101	22-23	0.5507120912454653
1101	24-25	0.533933693108672
1101	26-27	0.5721839419322379
1101	28-29	0.6525686790752729
1101	30-31	0.6973694814598446
1101	32-33	0.6478075007264792
1101	34-35	0.651987320903352
1101	36-37	0.5787769401806457
1101	38-39	0.6187755207466381
1101	40-41	0.6338252938297941
1101	42-43	0.7017487549249068
1101	44-45	0.5929181067043316
1101	46-47	0.5901187277432953
1101	48-49	0.5861179816477033
1101	50-51	0.5734241314590705
1101	52-53	0.5344061100983097
1101	54-55	0.5935762140655072
1101	56-57	0.5674317357910112
1101	58-59	0.5871469609081785
1101	60-61	0.5988484221788397
1101	62-63	0.6314091817356982
1101	64-65	0.551549347221922
1101	66-67	0.6195785484789056
1101	68-69	0.5518136889562157
1101	70-71	0.6298325958760529
1101	72-73	0.5628892298728481
1101	74-75	0.5748815911417431
1101	76-77	0.5380960709898446
1101	78-79	0.5056792209282115
1101	80-81	0.507777100171058
1101	82-83	0.4648125633603968
1101	84-85	0.4919735616649099
1101	86-87	0.5346672479684536
1101	88-89	0.48269474147583225
1101	90-91	0.4906800106121665
1101	92-93	0.4948071201798072
1101	94-95	0.47275324172758104
1101	96-97	0.5656025077690288
1101	98-99	0.6113161588577505
1101	100	0.6195675887111243
1102	1	0.2014591262660872
1102	2	0.2807117760761244
1102	3	0.2548406083251038
1102	4	0.25410908283600264
1102	5	0.18514238710341857
1102	6	0.26272516179821537
1102	7	0.3528867342622206
1102	8	0.277628789714889
1102	9	0.3599003635969069
1102	10-11	0.4094086327267803
1102	12-13	0.37365299403429475
1102	14-15	0.42974526429614457
1102	16-17	0.3942019523852309
1102	18-19	0.3791322671258044
1102	20-21	0.35371277721785077
1102	22-23	0.30921527492806433
1102	24-25	0.32929344629970814
1102	26-27	0.4155711065715906
1102	28-29	0.44775335914753356
1102	30-31	0.45023832668344
1102	32-33	0.5264958058421527
1102	34-35	0.5103406625931157
1102	36-37	0.46208445390696795
1102	38-39	0.4649355346347903
1102	40-41	0.4448327587121881
1102	42-43	0.43781609775632546
1102	44-45	0.4046758849528942
1102	46-47	0.34615360113159
1102	48-49	0.35227325826323863
1102	50-51	0.3267144762501317
1102	52-53	0.3381520863589813
1102	54-55	0.36172349910808066
1102	56-57	0.39946158355095207
1102	58-59	0.38563007738623867
1102	60-61	0.3971520519300924
1102	62-63	0.4107125966913472
1102	64-65	0.4287616682501181
1102	66-67	0.3764100781177504
1102	68-69	0.3735657148023108
1102	70-71	0.39078386081611427
1102	72-73	0.33578702836515717
1102	74-75	0.3161121197742851
1102	76-77	0.301940088197032
1102	78-79	0.23291456627832474
1102	80-81	0.26215025013368987
1102	82-83	0.2917862553140935
1102	84-85	0.2372620350861645
1102	86-87	0.22287526425761328
1102	88-89	0.3058330080459868
1102	90-91	0.1912181765520593
1102	92-93	0.18957486141290758
1102	94-95	0.30818009647279965
1102	96-97	0.28284360552162013
1102	98-99	0.27629609165321156
1102	100	0.27569897299515134
1103	1	0.39157460724645077
1103	2	0.41166171599600077
1103	3	0.41200486461726626
1103	4	0.3571812707563069
1103	5	0.2649562295261987
1103	6	0.37298223017484844
1103	7	0.3753979019005911
1103	8	0.2898955072369702
1103	9	0.48769394606440386
1103	10-11	0.5560439305894249
1103	12-13	0.44723041662078344
1103	14-15	0.6089924321180575
1103	16-17	0.5130360920445156
1103	18-19	0.44732022280512496
1103	20-21	0.42351123396638
1103	22-23	0.47679085066008753
1103	24-25	0.5561562647793394
1103	26-27	0.5264226431793659
1103	28-29	0.5882285919878996
1103	30-31	0.52546036504868
1103	32-33	0.6370663591241836
1103	34-35	0.5469074490376613
1103	36-37	0.5382471859706399
1103	38-39	0.5208521723846147
1103	40-41	0.4873956086418758
1103	42-43	0.4787299107365044
1103	44-45	0.41623425922388435
1103	46-47	0.4384850923901027
1103	48-49	0.4244014330223109
1103	50-51	0.3499879373720276
1103	52-53	0.38238440621658754
1103	54-55	0.4559453395960702
1103	56-57	0.41446393035619167
1103	58-59	0.37728816459180337
1103	60-61	0.45208802620796007
1103	62-63	0.41064042406589607
1103	64-65	0.4312475611306468
1103	66-67	0.4688313071936605
1103	68-69	0.4419507113083867
1103	70-71	0.4609770564192246
1103	72-73	0.45394825388501303
1103	74-75	0.4113468863316889
1103	76-77	0.4493672535908786
1103	78-79	0.39699478830235435
1103	80-81	0.3944163845391877
1103	82-83	0.4394638740571821
1103	84-85	0.4810937251778675
1103	86-87	0.3857890738920382
1103	88-89	0.4353703839066796
1103	90-91	0.405513458660991
1103	92-93	0.3855634495347289
1103	94-95	0.4413006520012672
1103	96-97	0.42436725328861513
1103	98-99	0.36464351059762024
1103	100	0.41594747146996625
1104	1	0.1642039925700871
1104	2	0.21234318716928158
1104	3	0.15569871346150777
1104	4	0.11668696143358659
1104	5	0.028305886883508435
1104	6	0.11564133161765966
1104	7	0.08916798859861075
1104	8	0.1409680499358288
1104	9	0.233127765573947
1104	10-11	0.2208388824322043
1104	12-13	0.15531265780101933
1104	14-15	0.17984269339105197
1104	16-17	0.22436234290126578
1104	18-19	0.12534358654036026
1104	20-21	0.11733621053213028
1104	22-23	0.1144570719509801
1104	24-25	0.22887262551984833
1104	26-27	0.34980776107872913
1104	28-29	0.41983398481048795
1104	30-31	0.3254831635881885
1104	32-33	0.433971710221698
1104	34-35	0.39506128745641433
1104	36-37	0.33021299798584636
1104	38-39	0.3445374277109181
1104	40-41	0.2971059082649603
1104	42-43	0.3068531372759651
1104	44-45	0.333564934557657
1104	46-47	0.3662889082125744
1104	48-49	0.2783092876248503
1104	50-51	0.2848187600727812
1104	52-53	0.28051148741406706
1104	54-55	0.2538574961587585
1104	56-57	0.28192940313837767
1104	58-59	0.30430194889507334
1104	60-61	0.20660505547445496
1104	62-63	0.25739436280978367
1104	64-65	0.2947405690006235
1104	66-67	0.3483339176119884
1104	68-69	0.32991883265093946
1104	70-71	0.2922081659943814
1104	72-73	0.29565591361637544
1104	74-75	0.3953284417642138
1104	76-77	0.3124673695195739
1104	78-79	0.35691625614687794
1104	80-81	0.361699324527887
1104	82-83	0.342486486509749
1104	84-85	0.4134599072864731
1104	86-87	0.3282233395479004
1104	88-89	0.32396478604171364
1104	90-91	0.37356166673624003
1104	92-93	0.41915520484312196
1104	94-95	0.4158393933369595
1104	96-97	0.37554480218845043
1104	98-99	0.42225146124875224
1104	100	0.40335007922990584
1105	1	0.3504845726889485
1105	2	0.38685590544872994
1105	3	0.4610553866628351
1105	4	0.3766955478469427
1105	5	0.35358281805296343
1105	6	0.4074652869373665
1105	7	0.37461357593359423
1105	8	0.437243126793625
1105	9	0.4783335742770589
1105	10-11	0.5346396356496186
1105	12-13	0.5036176147142442
1105	14-15	0.6052593886054254
1105	16-17	0.5949062485369225
1105	18-19	0.593397874332183
1105	20-21	0.5233400970721362
1105	22-23	0.5160425194721938
1105	24-25	0.5249380701236745
1105	26-27	0.5692835588858287
1105	28-29	0.6328637013151877
1105	30-31	0.662165686203501
1105	32-33	0.6626370699559487
1105	34-35	0.6007577428258486
1105	36-37	0.6007816019919474
1105	38-39	0.5625784822595037
1105	40-41	0.5558386884726758
1105	42-43	0.5104610332400199
1105	44-45	0.5479644160576385
1105	46-47	0.46842349388774096
1105	48-49	0.46949136158072236
1105	50-51	0.4901568128024252
1105	52-53	0.4795764855685931
1105	54-55	0.5503757718478255
1105	56-57	0.5207193818988856
1105	58-59	0.5952606600711192
1105	60-61	0.4854697702884039
1105	62-63	0.6012483086921705
1105	64-65	0.6385926362597587
1105	66-67	0.6506143176040311
1105	68-69	0.5821386194161491
1105	70-71	0.5643688479567288
1105	72-73	0.5651492820762805
1105	74-75	0.5609102127092065
1105	76-77	0.5257894605819686
1105	78-79	0.5989770837577773
1105	80-81	0.579134357315386
1105	82-83	0.49850896589462224
1105	84-85	0.5112965068457704
1105	86-87	0.45278600710451755
1105	88-89	0.5077083130795188
1105	90-91	0.4786638417399409
1105	92-93	0.4733666361551272
1105	94-95	0.5010799897224842
1105	96-97	0.5420096918248589
1105	98-99	0.5935653537782386
1105	100	0.6074886310102876
1106	1	0.10235369832085084
1106	2	0.17229720857548614
1106	3	0.19947721778060412
1106	4	0.21568768415100692
1106	5	0.07532312885618353
1106	6	0.18598073348295685
1106	7	0.1946384776373975
1106	8	0.1318001385891634
1106	9	0.28034199966555917
1106	10-11	0.27394257517405407
1106	12-13	0.19736607005968665
1106	14-15	0.39426303960711095
1106	16-17	0.3015868326971898
1106	18-19	0.35433646261153484
1106	20-21	0.32196703959225914
1106	22-23	0.3013340379144296
1106	24-25	0.26627283889356335
1106	26-27	0.3264994360623774
1106	28-29	0.4602144783232447
1106	30-31	0.3631148530261967
1106	32-33	0.36120962191251493
1106	34-35	0.33431709500707996
1106	36-37	0.315268027754918
1106	38-39	0.330148881061227
1106	40-41	0.4233517010171397
1106	42-43	0.33201561103444277
1106	44-45	0.3573246482238943
1106	46-47	0.30204800240674246
1106	48-49	0.2766735372032585
1106	50-51	0.3343006060995606
1106	52-53	0.33461149773859944
1106	54-55	0.3487754161091914
1106	56-57	0.37026384922710065
1106	58-59	0.4420001283354864
1106	60-61	0.4749706128272493
1106	62-63	0.44001080635178624
1106	64-65	0.5298179685945286
1106	66-67	0.5073987212737592
1106	68-69	0.491269212937965
1106	70-71	0.42585059294830785
1106	72-73	0.447839415010197
1106	74-75	0.4401355450439759
1106	76-77	0.4360343585411499
1106	78-79	0.4576567317637661
1106	80-81	0.3931406295490518
1106	82-83	0.36186433005863705
1106	84-85	0.40174885659300585
1106	86-87	0.4454027455430136
1106	88-89	0.46310365103121853
1106	90-91	0.4411234934905508
1106	92-93	0.5157137621614929
1106	94-95	0.519728370175585
1106	96-97	0.554673893285262
1106	98-99	0.5352718694120142
1106	100	0.5296217521486533
1107	1	0.06877215670176895
1107	2	0.09489674181943997
1107	3	0.13769275314316687
1107	4	0.15702565194152385
1107	5	0.029242058958637074
1107	6	0.05560828864127387
1107	7	0.21989070796991683
1107	8	0.19046495147295417
1107	9	0.3121836336427535
1107	10-11	0.31079968492792887
1107	12-13	0.25493470140516195
1107	14-15	0.3789433117159575
1107	16-17	0.2706721563643626
1107	18-19	0.2952723581376304
1107	20-21	0.2854069985442109
1107	22-23	0.31920973515195783
1107	24-25	0.2931356755123957
1107	26-27	0.32644018121689555
1107	28-29	0.4111353118634682
1107	30-31	0.3495030550223177
1107	32-33	0.3355692888936588
1107	34-35	0.3140093008431961
1107	36-37	0.2992367120656283
1107	38-39	0.3659190781911761
1107	40-41	0.2910918403818883
1107	42-43	0.37539618272784026
1107	44-45	0.33240590018939997
1107	46-47	0.30102656804223926
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2216	90-91	-1.7809898225541012
2216	92-93	-1.653870914803214
2216	94-95	-1.6099899182447572
2216	96-97	-1.6878639351057672
2216	98-99	-2.054060693447383
2216	100	-2.044923517893068
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	138671.0
3	29989.0
4	20561.0
5	18081.0
6	12299.0
7	11381.0
8	12393.0
9	12806.0
10	13173.0
11	13445.0
12	14864.0
13	16375.0
14	17649.0
15	18082.0
16	17822.0
17	17485.0
18	16383.0
19	15048.0
20	14359.0
21	15571.0
22	19373.0
23	29652.0
24	46438.0
25	55738.0
26	59285.0
27	47929.0
28	49816.0
29	59998.0
30	68880.0
31	79622.0
32	99362.0
33	129973.0
34	177364.0
35	274756.0
36	422394.0
37	737576.0
38	946067.0
39	382588.0
40	1151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.84128379533996	21.393516723221545	12.928694406014113	21.836505075424377
2	16.49466032486763	23.146994003221536	37.92181667724144	22.4365289946694
3	17.629664821537382	28.641271888584928	29.58980848085377	24.139254809023917
4	12.41024122659015	20.318229548787773	37.220056037776516	30.051473186845563
5	11.849643375936312	39.83659518058591	32.68749523755384	15.626266205923939
6	27.520914436773126	40.22248417764771	18.46053586145853	13.796065524120635
7	25.222282105963906	35.556549496654036	21.615428976162185	17.605739421219873
8	23.669759345767265	37.41840958437025	20.686617333452023	18.225213736410467
9	24.15333773895072	20.749756417091344	21.10886583733983	33.98804000661811
10-11	22.033291329429012	30.27610703509158	27.726386591453917	19.964215044025487
12-13	23.056023780689948	28.52143163565657	27.19900689640492	21.223537687248566
14-15	21.213096477360647	30.428692184597107	25.105613144033363	23.252598194008886
16-17	20.124716388739724	33.18771799970006	25.42805344711648	21.259512164443738
18-19	19.98662119339874	31.66015605866308	27.57137167599486	20.781851071943315
20-21	22.18501597179791	31.08558638969639	26.485089360310504	20.244308278195195
22-23	21.529366346591804	31.393976542141683	26.62402092625377	20.452636185012736
24-25	21.09650675978362	31.03234372384593	27.0482294330451	20.82292008332535
26-27	20.920939516599155	30.70929623335138	27.151071350994137	21.218692899055327
28-29	20.928298929044026	30.857599384569234	27.16432533644274	21.049776349943997
30-31	21.45094683626974	31.709614704308308	26.3892989208818	20.450139538540153
32-33	20.518411192573115	31.081353693728126	27.393684826700404	21.00655028699836
34-35	20.74715850749713	30.24324273985936	27.43812985540311	21.571468897240397
36-37	21.55543944819979	31.06469235783803	26.589346424437693	20.79052176952448
38-39	21.236357989386214	30.93055030289778	27.04053710671636	20.79255460099965
40-41	20.734485183329824	30.62875393580463	27.365588809918144	21.271172070947404
42-43	20.644604328380034	30.7241860204667	27.317210101786827	21.313999549366446
44-45	21.258936406744503	30.873174863795082	26.982772049425623	20.885116680034795
46-47	20.88062923132316	31.549565464118327	26.694957226120604	20.874848078437907
48-49	20.610529060522456	30.706353341392866	27.408076078906028	21.275041519178643
50-51	20.701642143760747	30.331053182817598	27.391001468229874	21.576303205191778
52-53	20.689087167400537	30.586415632138536	27.18426452959326	21.540232670867667
54-55	21.03027312451396	31.19718590922779	26.866209745790897	20.906331220467347
56-57	20.493568616182152	30.902340879548397	26.89451166327791	21.70957884099154
58-59	20.440981472460244	30.187253977598992	27.506084496494914	21.86568005344585
60-61	21.16172315188434	30.957663782297974	26.61537627974006	21.265236786077626
62-63	20.84448660174641	30.86423261864935	26.87273621355643	21.41854456604781
64-65	20.72647345030745	30.45520876998668	27.328623665428132	21.48969411427774
66-67	20.622558728686705	30.572369389376945	26.85519688059088	21.94987500134547
68-69	21.420654964236483	30.93061096341505	26.697524406535567	20.951209665812904
70-71	21.41036691803053	30.484972894863414	26.91754192095433	21.187118266151725
72-73	20.898498064710882	30.642815435678305	27.4419933308597	21.016693168751114
74-75	20.91155489962996	30.5949662534666	27.175383649351947	21.318095197551497
76-77	21.055856226303284	30.712615875818617	26.71169673038199	21.519831167496115
78-79	21.337396627222578	30.889725182218726	26.82385928180176	20.949018908756937
80-81	20.929498188291866	30.544746046412623	27.1150906337834	21.41066513151211
82-83	20.75185072846356	30.63835437912613	27.27313186842425	21.33666302398606
84-85	20.96585390513631	30.465634943162407	27.002903407957785	21.5656077437435
86-87	21.435379466890776	30.52751334659719	26.792836551154387	21.244270635357648
88-89	20.993890288353377	30.38763694968422	27.03241730591939	21.586055456043006
90-91	20.8770652951085	30.190969349893425	26.957077853534695	21.974887501463375
92-93	21.13541423149352	30.593365702466098	26.678520379020476	21.592699687019905
94-95	21.044654674644146	30.295171811264964	26.959338258628296	21.70083525546259
96-97	21.04218821429349	30.341470081004662	26.92172613216652	21.694615572535334
98-99	20.958850531674816	30.350030742854766	26.9460033563167	21.745115369153716
100	21.212632094281375	30.665489834584786	26.502958326550583	21.618919744583252
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	42407.0
1	31290.0
2	17922.0
3	13086.0
4	8709.5
5	6063.0
6	4797.0
7	4452.0
8	4598.5
9	4475.0
10	3727.5
11	2977.5
12	2736.5
13	2725.5
14	2769.5
15	2897.5
16	3183.0
17	3562.5
18	4254.5
19	5167.5
20	6373.0
21	8124.0
22	10609.0
23	14809.5
24	20107.0
25	26086.5
26	34026.5
27	44430.0
28	57611.0
29	69829.0
30	78404.5
31	86919.0
32	95850.5
33	103925.0
34	111933.0
35	118711.5
36	123037.0
37	126544.5
38	128905.5
39	129354.0
40	130560.5
41	129665.5
42	125844.0
43	123745.0
44	122304.5
45	121539.0
46	122420.0
47	122798.0
48	123707.0
49	125656.0
50	127515.5
51	124347.5
52	117168.5
53	111639.5
54	107670.5
55	104729.0
56	97743.0
57	88444.0
58	81761.5
59	75554.5
60	65843.0
61	54486.5
62	44565.0
63	34950.5
64	26399.5
65	19736.5
66	14679.5
67	10845.0
68	8055.0
69	5914.5
70	4276.5
71	3124.0
72	2420.0
73	1899.5
74	1483.0
75	1150.5
76	793.0
77	605.5
78	496.0
79	360.5
80	262.5
81	187.5
82	134.5
83	104.0
84	93.5
85	79.0
86	64.5
87	54.5
88	42.0
89	36.5
90	33.5
91	26.5
92	23.0
93	17.5
94	19.5
95	21.0
96	19.0
97	16.5
98	17.0
99	16.5
100	26.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01586687690278563
2	0.008368810073725346
3	0.010932665183016927
4	0.00972329956542656
5	0.011948532301792837
6	0.012867650171161517
7	0.011198725618886808
8	0.008102749637855466
9	0.006845009395561484
10-11	0.00627660755529401
12-13	0.006978039613496424
14-15	0.007872970170513296
16-17	0.005297021405045812
18-19	0.006010547119424129
20-21	0.005732393027378345
22-23	0.006337075836173529
24-25	0.005877516901489188
26-27	0.006603136272043409
28-29	0.01714880445743142
30-31	0.005720299371202441
32-33	0.005538894528563885
34-35	0.00522445946799039
36-37	0.007111069831431364
38-39	0.006010547119424129
40-41	0.005756580339730152
42-43	0.005309115061221716
44-45	0.004523027409787976
46-47	0.006663604552922928
48-49	0.003990906538048214
50-51	0.004087655787455444
52-53	0.003555534915715682
54-55	0.004934211719768702
56-57	0.003410411041604838
58-59	0.0029266647945686906
60-61	0.0038941572886409853
62-63	0.0035676285718915855
64-65	0.005030960969175931
66-67	0.003736939758354237
68-69	0.003821595351585563
70-71	0.005309115061221716
72-73	0.003216912542790379
74-75	0.002962945763096402
76-77	0.0029992267316241127
78-79	0.003640190508947008
80-81	0.004329528910973517
82-83	0.0019591723004963963
84-85	0.00289038382604098
86-87	0.0025517614531156765
88-89	0.001922891331968685
90-91	0.0029750394192723053
92-93	0.0026726980148747133
94-95	0.002297794673421699
96-97	0.001668924552274708
98-99	0.002539667796939773
100	0.003289474479845801
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4134399.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.5090040894646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.46487520143705	51.40155830450269
2	14.137162236480243	18.522228375260173
3	3.9421191275705274	7.747328941475226
4	1.4201430840999203	3.7212863641570606
5	0.6451382183436633	2.113118109187247
6	0.35440702753079867	1.393011084951005
7	0.21480222128008938	0.9850035714584418
8	0.14061999250967427	0.7369500531501386
9	0.10559293715608387	0.6225559336778829
>10	0.513509897270937	6.016799282076116
>50	0.03650495234473186	1.644291711826902
>100	0.023348638190014537	2.749540487469204
>500	0.0013693591449582057	0.5983831583592386
>1k	3.3308725212691156E-4	0.3770775453970677
>5k	3.700969468075937E-5	0.16704586174920824
>10k+	3.700969468075937E-5	1.2038212153023855
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49653	1.2009726202042907	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6890	0.16665058210395273	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.256193705542208E-5	0.0
5	0.0	0.0	0.0	7.256193705542208E-5	0.0
6	0.0	0.0	0.0	7.256193705542208E-5	0.0
7	0.0	0.0	0.0	7.256193705542208E-5	0.0
8	0.0	0.0	0.0	9.674924940722944E-5	0.0
9	0.0	0.0	0.0	9.674924940722944E-5	0.0
10-11	0.0	0.0	0.0	9.674924940722944E-5	0.0
12-13	0.0	0.0	0.0	9.674924940722944E-5	0.0
14-15	0.0	0.0	0.0	2.0559215499036255E-4	0.0
16-17	0.0	0.0	0.0	3.02341404397592E-4	0.0
18-19	0.0	0.0	0.0	3.3862237292530306E-4	0.0
20-21	0.0	0.0	0.0	4.8374624703614725E-4	0.0
22-23	0.0	0.0	0.0	0.0010279607749518129	0.0
24-25	0.0	0.0	0.0	0.002491293172236158	0.0
26-27	0.0	0.0	0.0	0.004329528910973517	0.0
28-29	0.0	0.0	0.0	0.008997680194872338	0.0
30-31	0.0	0.0	0.0	0.0193861308499736	0.0
32-33	0.0	0.0	0.0	0.0404169989398701	0.0
34-35	0.0	0.0	0.0	0.07287637211599557	0.0
36-37	0.0	0.0	0.0	0.11909832602029945	0.0
38-39	0.0	0.0	0.0	0.18458547421281787	0.0
40-41	0.0	0.0	0.0	0.26309749010678457	0.0
42-43	0.0	0.0	0.0	0.3430365574295079	0.0
44-45	0.0	0.0	0.0	0.43793547744182404	0.0
46-47	0.0	0.0	0.0	0.5525228697085114	0.0
48-49	0.0	0.0	0.0	0.6780066461896881	0.0
50-51	0.0	0.0	0.0	0.810105652599084	0.0
52-53	0.0	0.0	0.0	0.9431358705340244	1.209365617590368E-5
54-55	0.0	0.0	0.0	1.0957699051301049	2.418731235180736E-5
56-57	0.0	0.0	0.0	1.2715632913030406	2.418731235180736E-5
58-59	0.0	0.0	0.0	1.4678554246941333	2.418731235180736E-5
60-61	0.0	0.0	0.0	1.6694566731464477	2.418731235180736E-5
62-63	0.0	0.0	0.0	1.8756172299770777	2.418731235180736E-5
64-65	0.0	0.0	0.0	2.107682398336493	2.418731235180736E-5
66-67	0.0	0.0	0.0	2.3818697711565813	2.418731235180736E-5
68-69	0.0	0.0	0.0	2.678224815747101	2.418731235180736E-5
70-71	0.0	0.0	0.0	3.0006295957405174	2.418731235180736E-5
72-73	0.0	0.0	0.0	3.357900386489064	2.418731235180736E-5
74-75	0.0	0.0	0.0	3.723273926875466	2.418731235180736E-5
76-77	0.0	0.0	0.0	4.103196135641481	2.418731235180736E-5
78-79	0.0	0.0	0.0	4.528989582282698	2.418731235180736E-5
80-81	0.0	0.0	0.0	4.990326284424895	2.418731235180736E-5
82-83	0.0	0.0	0.0	5.4788737129628755	2.418731235180736E-5
84-85	0.0	2.418731235180736E-5	0.0	5.966647147505599	2.418731235180736E-5
86-87	0.0	2.418731235180736E-5	0.0	6.485392435514811	2.418731235180736E-5
88	0.0	2.418731235180736E-5	0.0	6.903083132518173	2.418731235180736E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	4740	0.0	41.452316	2
GGTATCA	7105	0.0	39.56767	1
GTACCTG	5610	0.0	36.452705	1
TCTGTCG	1355	0.0	30.524387	8
GTATCAA	10710	0.0	30.19968	1
ACCTGGG	6565	0.0	29.284609	3
GTCTCGC	1200	0.0	28.990376	1
ATCAACG	11760	0.0	26.061003	3
TCAACGC	11660	0.0	26.042627	4
TATCAAC	12580	0.0	25.146948	2
CTGTCGC	1625	0.0	24.584938	9
GTACATG	18145	0.0	24.431948	1
CAACGCA	12620	0.0	24.061281	5
TCGCTCT	1480	0.0	23.820475	4
TACATGG	18235	0.0	23.225756	2
CATGGGT	8645	0.0	22.673672	4
AACGCAG	13590	0.0	22.549501	6
CGCTCTG	1300	0.0	22.056269	5
TCTCGCT	1605	0.0	21.965296	2
TATAACG	285	9.640644E-11	21.441212	2
>>END_MODULE