##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431438_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4134399
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26830259972489	33.0	31.0	34.0	30.0	34.0
2	32.36995776169644	34.0	31.0	34.0	31.0	34.0
3	32.469177745060406	34.0	31.0	34.0	30.0	34.0
4	36.02930679888419	37.0	35.0	37.0	35.0	37.0
5	35.951424620603866	37.0	35.0	37.0	35.0	37.0
6	35.99881554731413	37.0	35.0	37.0	35.0	37.0
7	35.93957477253647	37.0	35.0	37.0	35.0	37.0
8	35.92495813780915	37.0	35.0	37.0	35.0	37.0
9	37.61283683553523	39.0	37.0	39.0	35.0	39.0
10-11	37.544776036371914	39.0	37.0	39.0	35.0	39.0
12-13	37.525983824976734	39.0	37.0	39.0	35.0	39.0
14-15	38.8468695208179	40.0	38.0	41.0	35.0	41.0
16-17	38.89947692518308	40.0	38.0	41.0	35.0	41.0
18-19	38.99626862332349	40.5	38.5	41.0	35.0	41.0
20-21	38.995409489988745	41.0	38.5	41.0	35.0	41.0
22-23	38.89095307443718	40.0	38.0	41.0	35.0	41.0
24-25	38.81539880403415	40.0	38.0	41.0	35.0	41.0
26-27	38.651707660533006	40.0	38.0	41.0	34.5	41.0
28-29	38.53182808432375	40.0	38.0	41.0	34.0	41.0
30-31	38.2931429695102	40.0	37.5	41.0	34.0	41.0
32-33	38.002664958074924	40.0	37.0	41.0	33.5	41.0
34-35	37.79070936307792	40.0	37.0	41.0	33.0	41.0
36-37	37.598498959582756	40.0	37.0	41.0	33.0	41.0
38-39	37.46758041495269	40.0	36.0	41.0	33.0	41.0
40-41	37.31395070964365	40.0	36.0	41.0	33.0	41.0
42-43	37.151456596230794	40.0	36.0	41.0	32.0	41.0
44-45	36.88224370700554	40.0	36.0	41.0	31.0	41.0
46-47	36.48030789964878	40.0	36.0	41.0	30.0	41.0
48-49	36.152625931846444	40.0	35.0	41.0	29.0	41.0
50-51	35.9921405747244	40.0	35.0	41.0	28.5	41.0
52-53	35.84590480986475	40.0	35.0	41.0	28.0	41.0
54-55	35.68035849950621	39.5	35.0	41.0	28.0	41.0
56-57	35.46191756044833	39.0	35.0	41.0	27.0	41.0
58-59	35.29423006826386	39.0	35.0	41.0	27.0	41.0
60-61	35.07376235336744	39.0	35.0	41.0	26.0	41.0
62-63	34.81990223972093	38.0	35.0	41.0	26.0	41.0
64-65	34.56023753391968	37.5	35.0	40.5	26.0	41.0
66-67	34.2901326166149	37.0	34.5	40.0	26.0	41.0
68-69	33.98901654146104	37.0	34.0	40.0	25.0	41.0
70-71	33.64449863208655	36.0	34.0	39.0	24.5	41.0
72-73	33.315019667913035	36.0	34.0	39.0	24.0	41.0
74-75	33.00947840302786	35.0	34.0	39.0	23.5	40.5
76-77	32.666238914047725	35.0	33.5	37.5	22.5	40.0
78-79	32.35881031317974	35.0	33.0	37.0	22.0	39.0
80-81	32.0635071748034	35.0	33.0	36.5	21.0	39.0
82-83	31.817309722646506	35.0	33.0	36.0	20.0	38.5
84-85	31.59550831934702	35.0	33.0	36.0	20.0	37.0
86-87	31.355732719556094	35.0	33.0	35.5	19.5	37.0
88-89	31.20946551119038	35.0	33.0	35.0	19.5	36.5
90-91	31.027719022764856	35.0	33.0	35.0	17.5	36.0
92-93	30.833310960069404	35.0	33.0	35.0	13.0	36.0
94-95	30.669728780410406	35.0	33.0	35.0	4.5	36.0
96-97	30.499849796790294	35.0	33.0	35.0	2.0	36.0
98-99	30.335551188939434	35.0	33.0	35.0	2.0	35.0
100	30.223161334936467	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19454013975271067
1101	2	0.26051660393061127
1101	3	0.2447065426770081
1101	4	0.15530398952176938
1101	5	0.20572615285630036
1101	6	0.23169528021549723
1101	7	0.22059421497014853
1101	8	0.2733657238644298
1101	9	0.3500248095566363
1101	10-11	0.3350449204306898
1101	12-13	0.3193443868012267
1101	14-15	0.41531274781051764
1101	16-17	0.40354249704030565
1101	18-19	0.4127081151466072
1101	20-21	0.3390073480321618
1101	22-23	0.4258981869766245
1101	24-25	0.416100408639295
1101	26-27	0.43471449014551666
1101	28-29	0.5191854182318494
1101	30-31	0.46494543927052945
1101	32-33	0.5209402926440845
1101	34-35	0.6191992720608752
1101	36-37	0.6176702105447731
1101	38-39	0.5868469555041003
1101	40-41	0.6097723023242239
1101	42-43	0.6791523957718866
1101	44-45	0.7275455653730418
1101	46-47	0.7125885542142285
1101	48-49	0.653550457784803
1101	50-51	0.6878068392909498
1101	52-53	0.6590425830479134
1101	54-55	0.6646481086691907
1101	56-57	0.6909564159860082
1101	58-59	0.7203562311588598
1101	60-61	0.7797863959026969
1101	62-63	0.739143557536508
1101	64-65	0.715502938685745
1101	66-67	0.7042396046240214
1101	68-69	0.6595471308646879
1101	70-71	0.6789635608634867
1101	72-73	0.6539247239298192
1101	74-75	0.5434970274029354
1101	76-77	0.6091888941298507
1101	78-79	0.5977296400502752
1101	80-81	0.6307295299989732
1101	82-83	0.5838245741621648
1101	84-85	0.5157796346529899
1101	86-87	0.5246987694882641
1101	88-89	0.47819151506945445
1101	90-91	0.5342301453955614
1101	92-93	0.5444302928027405
1101	94-95	0.5447382492686401
1101	96-97	0.6064690988656309
1101	98-99	0.6846096338401821
1101	100	0.753425959512505
1102	1	0.1250390540051285
1102	2	0.22219692335814045
1102	3	0.18810054840881207
1102	4	0.14550357287716764
1102	5	0.128159198422388
1102	6	0.1099289837034263
1102	7	0.14023491785194153
1102	8	0.22614085347738921
1102	9	0.22125191028694502
1102	10-11	0.28107532136284163
1102	12-13	0.2777553260758623
1102	14-15	0.3620684516632977
1102	16-17	0.39748298100713697
1102	18-19	0.35310049961305623
1102	20-21	0.2786575107471805
1102	22-23	0.24838985996946406
1102	24-25	0.21571929358535158
1102	26-27	0.21444271487467148
1102	28-29	0.20593069125764174
1102	30-31	0.19289321451783792
1102	32-33	0.2126802987642975
1102	34-35	0.2148833518824489
1102	36-37	0.25953203224677424
1102	38-39	0.24184220351944674
1102	40-41	0.32095130943896066
1102	42-43	0.29565877490669834
1102	44-45	0.31390920842396497
1102	46-47	0.31161005731258484
1102	48-49	0.25345578589242734
1102	50-51	0.23670262812039766
1102	52-53	0.3229941451709166
1102	54-55	0.2695113898042507
1102	56-57	0.34235604465445135
1102	58-59	0.34575823729083055
1102	60-61	0.3145972580863088
1102	62-63	0.29650280757623193
1102	64-65	0.3622554058926397
1102	66-67	0.3717422822129066
1102	68-69	0.39486305692793167
1102	70-71	0.341286869421765
1102	72-73	0.3165812598948392
1102	74-75	0.3394813061311055
1102	76-77	0.33738346362652294
1102	78-79	0.33468007228962904
1102	80-81	0.27511887790309686
1102	82-83	0.29525037552626543
1102	84-85	0.2032774278489704
1102	86-87	0.1538045283640308
1102	88-89	0.19764088804287283
1102	90-91	0.16337194873695537
1102	92-93	0.16414904298664013
1102	94-95	0.1630906199646347
1102	96-97	0.18007336888428682
1102	98-99	0.27909505127922785
1102	100	0.2976098539625376
1103	1	0.17143135476327842
1103	2	0.22762217785028582
1103	3	0.17223105467827082
1103	4	0.19789934556877142
1103	5	0.15767397851574572
1103	6	0.17732435490491838
1103	7	0.18744059453960915
1103	8	0.19155128629327578
1103	9	0.2849441327545179
1103	10-11	0.25952121770383485
1103	12-13	0.2738361971755552
1103	14-15	0.394434528426288
1103	16-17	0.3629250192194391
1103	18-19	0.3458080771651808
1103	20-21	0.34125394006628085
1103	22-23	0.3223391388645567
1103	24-25	0.29887135891735284
1103	26-27	0.3985850149158736
1103	28-29	0.3624402558033921
1103	30-31	0.29367105379370173
1103	32-33	0.29190021066588656
1103	34-35	0.30817322152243065
1103	36-37	0.35512049246531774
1103	38-39	0.31403526194677767
1103	40-41	0.32348093606272244
1103	42-43	0.29488351663863455
1103	44-45	0.27013507719597385
1103	46-47	0.29235968234950604
1103	48-49	0.3138144456037537
1103	50-51	0.3438319571635233
1103	52-53	0.35295062412183853
1103	54-55	0.30523875125909683
1103	56-57	0.30148226119336385
1103	58-59	0.3480524066986277
1103	60-61	0.36426176291996626
1103	62-63	0.33513493796874627
1103	64-65	0.2520229526829638
1103	66-67	0.2685520370840777
1103	68-69	0.2230548076446368
1103	70-71	0.35535007591290935
1103	72-73	0.3794276645987651
1103	74-75	0.36529042817740276
1103	76-77	0.36126208743316823
1103	78-79	0.3455943895222475
1103	80-81	0.2934771323006089
1103	82-83	0.25535074327207496
1103	84-85	0.25420680574005416
1103	86-87	0.3012705283659436
1103	88-89	0.3019532143625554
1103	90-91	0.3131815277172265
1103	92-93	0.29992277893807184
1103	94-95	0.4039191999419458
1103	96-97	0.4265682169059417
1103	98-99	0.4877757452453082
1103	100	0.4366598566869371
1104	1	0.09416320348499596
1104	2	0.15692607400221448
1104	3	0.1467461017143492
1104	4	0.12470366648436482
1104	5	0.073356773102347
1104	6	0.14388170031117653
1104	7	0.12750222283499824
1104	8	0.10948359456882883
1104	9	0.25623895110015127
1104	10-11	0.21672757887200333
1104	12-13	0.18798100055332156
1104	14-15	0.27271491752136967
1104	16-17	0.21670899886776596
1104	18-19	0.23397341020878315
1104	20-21	0.22846303474754848
1104	22-23	0.20733284542741615
1104	24-25	0.20000839634394652
1104	26-27	0.2644612069992007
1104	28-29	0.2137411884341418
1104	30-31	0.18138740875511417
1104	32-33	0.16183968617811928
1104	34-35	0.28165788437566874
1104	36-37	0.2354017206853456
1104	38-39	0.2573562434224499
1104	40-41	0.22861404601467683
1104	42-43	0.21215163324232833
1104	44-45	0.2508360701604886
1104	46-47	0.21494569554665333
1104	48-49	0.23240369278384065
1104	50-51	0.22986639249368324
1104	52-53	0.23434869182280949
1104	54-55	0.2162887034659562
1104	56-57	0.2224510806508917
1104	58-59	0.23712488467159432
1104	60-61	0.13140730486638574
1104	62-63	0.17150395414675046
1104	64-65	0.1658853893585075
1104	66-67	0.11401151578637325
1104	68-69	0.14378286990169897
1104	70-71	0.15067495943519305
1104	72-73	0.18664145190717818
1104	74-75	0.1643525962404908
1104	76-77	0.16273590176462704
1104	78-79	0.22678296494004968
1104	80-81	0.22164994349633105
1104	82-83	0.236497044591907
1104	84-85	0.24431018801464077
1104	86-87	0.2365813340068037
1104	88-89	0.17842798224510759
1104	90-91	0.20187449235955413
1104	92-93	0.2437137166197516
1104	94-95	0.1683271545228635
1104	96-97	0.1588685173034321
1104	98-99	0.1551455450689332
1104	100	0.20675013774756223
1105	1	0.12829320200420113
1105	2	0.1299550114308019
1105	3	0.1307380214242002
1105	4	0.10306529704094203
1105	5	0.08733245929712297
1105	6	0.10452520613159066
1105	7	0.15001404158542186
1105	8	0.12614377814347222
1105	9	0.13963407640432024
1105	10-11	0.13027154086634596
1105	12-13	0.09558804340140625
1105	14-15	0.19812503755434818
1105	16-17	0.17373734287241405
1105	18-19	0.1788646600236845
1105	20-21	0.16470452292658422
1105	22-23	0.127493760560057
1105	24-25	0.18788732570550337
1105	26-27	0.21540739770377826
1105	28-29	0.2348059423676503
1105	30-31	0.21476628949348253
1105	32-33	0.23325121186331899
1105	34-35	0.2649747705465657
1105	36-37	0.25316631372029974
1105	38-39	0.3054124485150709
1105	40-41	0.34100823532243396
1105	42-43	0.3024756460258047
1105	44-45	0.270109363431267
1105	46-47	0.27534362662991185
1105	48-49	0.3222912295551339
1105	50-51	0.2668600482724415
1105	52-53	0.24355438780852268
1105	54-55	0.29578563025933136
1105	56-57	0.3596782935033289
1105	58-59	0.300710839421086
1105	60-61	0.2889089790512287
1105	62-63	0.2896748547145407
1105	64-65	0.2912224640989791
1105	66-67	0.33380924884063745
1105	68-69	0.34642327488867153
1105	70-71	0.3284146828134453
1105	72-73	0.32853400460255955
1105	74-75	0.3330017998147383
1105	76-77	0.31323680350379135
1105	78-79	0.2578617753958028
1105	80-81	0.23621499248420008
1105	82-83	0.21380482275522894
1105	84-85	0.20162339208395608
1105	86-87	0.17775777209290666
1105	88-89	0.2019137566467677
1105	90-91	0.188975229663928
1105	92-93	0.22867426000600233
1105	94-95	0.19857721585438526
1105	96-97	0.17145634294067236
1105	98-99	0.2541911768278311
1105	100	0.2668275149242767
1106	1	0.11122842012814971
1106	2	0.17636579592808488
1106	3	0.11363036368220492
1106	4	0.1287086222609375
1106	5	0.10431523405840437
1106	6	0.11589835722688946
1106	7	0.09368882158916847
1106	8	0.12431003914328898
1106	9	0.17098307471924556
1106	10-11	0.1318513012108511
1106	12-13	0.10779006549464043
1106	14-15	0.19701391604152008
1106	16-17	0.1594762494442179
1106	18-19	0.16065758269733976
1106	20-21	0.15300961560585336
1106	22-23	0.1344167150629545
1106	24-25	0.1726370916669282
1106	26-27	0.20380367180974446
1106	28-29	0.1543186740658271
1106	30-31	0.2046552615960877
1106	32-33	0.18309721448455463
1106	34-35	0.2218107563170264
1106	36-37	0.2515235501928643
1106	38-39	0.17731480762385132
1106	40-41	0.22523338042635288
1106	42-43	0.30490449830065813
1106	44-45	0.21092437934589015
1106	46-47	0.2363826642633171
1106	48-49	0.23025247276801508
1106	50-51	0.24472945475063312
1106	52-53	0.25923384858366205
1106	54-55	0.2947030614519903
1106	56-57	0.36775796023240304
1106	58-59	0.3581561680112344
1106	60-61	0.2520585765048935
1106	62-63	0.14382903932416724
1106	64-65	0.2112527018821666
1106	66-67	0.1982942703721875
1106	68-69	0.24266424099988626
1106	70-71	0.20909723289770454
1106	72-73	0.3048688669878814
1106	74-75	0.2990894238551789
1106	76-77	0.27071545918625617
1106	78-79	0.25584370761823294
1106	80-81	0.263363747399012
1106	82-83	0.21632008206997
1106	84-85	0.27974041136873495
1106	86-87	0.30257334970009353
1106	88-89	0.26355315001879376
1106	90-91	0.27340183516851724
1106	92-93	0.3159246812754297
1106	94-95	0.36737715968512674
1106	96-97	0.3513086177955671
1106	98-99	0.3492717797111844
1106	100	0.3891802578563137
1107	1	0.09524311797809304
1107	2	0.11192102045098551
1107	3	0.0671312657461911
1107	4	0.10152038054089729
1107	5	0.079408999058316
1107	6	0.07601011385168022
1107	7	0.02839421907663109
1107	8	-0.05059225656818711
1107	9	0.07305831844203681
1107	10-11	0.009410597435426382
1107	12-13	-0.06824403086896069
1107	14-15	0.0955633971478278
1107	16-17	0.06451244786519794
1107	18-19	0.030823727987574046
1107	20-21	0.017900292071090007
1107	22-23	0.048461503781368265
1107	24-25	0.06531080932917632
1107	26-27	0.020281749172077923
1107	28-29	0.05696594221883089
1107	30-31	0.07944606550888977
1107	32-33	0.010690772589057929
1107	34-35	0.045508854534411114
1107	36-37	0.04944115616469702
1107	38-39	-0.022521596037371694
1107	40-41	0.07733944328945341
1107	42-43	0.08440497002219871
1107	44-45	0.0606783227724037
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2216	70-71	-1.1614650627387846
2216	72-73	-0.9853572577161458
2216	74-75	-1.000734795335262
2216	76-77	-0.9566429239460064
2216	78-79	-0.9695896557823751
2216	80-81	-1.0098746109277492
2216	82-83	-0.9988584611705313
2216	84-85	-1.0399398795321524
2216	86-87	-1.0477730816901385
2216	88-89	-1.0224818031890095
2216	90-91	-1.0968487567809717
2216	92-93	-1.044678599816013
2216	94-95	-1.0868081486923664
2216	96-97	-1.3087552563728835
2216	98-99	-1.3957803118436694
2216	100	-1.3837027052736914
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	253.0
3	890.0
4	645.0
5	505.0
6	938.0
7	2086.0
8	3350.0
9	4936.0
10	6893.0
11	9618.0
12	13867.0
13	19719.0
14	19619.0
15	23324.0
16	29830.0
17	42389.0
18	47707.0
19	27052.0
20	17548.0
21	15923.0
22	15784.0
23	16283.0
24	17753.0
25	21374.0
26	26232.0
27	32680.0
28	41299.0
29	51410.0
30	64636.0
31	79949.0
32	99818.0
33	127301.0
34	173292.0
35	271139.0
36	396621.0
37	750410.0
38	1126162.0
39	531795.0
40	3369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.20555929565283	20.378647340984426	13.906128021674602	23.509665341688134
2	14.92422477849864	21.759825309555268	39.41487021451002	23.901079697436074
3	16.410216346095705	27.563047933989843	31.370861122573224	24.65587459734123
4	11.212266585081778	18.58063591677954	37.908678818555266	32.29841867958341
5	11.086739717955684	37.19263804393285	34.6775280086247	17.04309422948677
6	27.27025137148108	38.76904478740441	18.85471141029204	15.105992430822473
7	24.290229365864302	33.81388685513904	23.401660071995952	18.494223707000703
8	24.90862638076296	33.01589904602821	22.187602115809334	19.887872457399492
9	24.95184910793564	15.616804280380292	23.547122568479722	35.88422404320434
10-11	22.540174522805458	26.615780106974018	29.561182040885836	21.28286332933469
12-13	24.67800035748848	24.892783691172525	29.815603186823523	20.61361276451547
14-15	22.05367696731738	26.243657179677143	29.269840670917347	22.432825182088134
16-17	19.170513054013412	29.237768294738846	29.61846449750012	21.973254153747618
18-19	18.78213737958044	26.19339594461009	34.621452839941185	20.403013835868283
20-21	20.71724330428679	25.66888198260497	34.2245511379042	19.38932357520404
22-23	20.094359542946872	26.077768497912274	34.35522309288484	19.472648866256016
24-25	19.713710329894766	25.732594907239996	34.69746000548087	19.85623475738436
26-27	19.504576118560397	25.50283608330981	34.811323241902876	20.181264556226918
28-29	19.56136633655703	25.65107643605432	34.7305426878587	20.057014539529952
30-31	20.25106645858348	26.42562945002762	33.98328690807799	19.340017183310902
32-33	19.282252471191356	25.850339560165125	34.858325618576366	20.00908235006715
34-35	19.498149462120722	25.294022248057335	34.57669225681448	20.631136033007458
36-37	20.472458467043698	26.175601022386907	33.482324592604165	19.86961591796523
38-39	20.227099950729823	25.978153260962372	33.83431531025335	19.960431478054456
40-41	19.731684902277998	25.88362321527503	33.98411191296927	20.400579969477707
42-43	19.725902084874548	26.037185509462184	33.686341339113234	20.550571066550035
44-45	20.38187658978688	26.242172833605665	33.093423384320594	20.28252719228686
46-47	20.191941625132852	27.06426256693262	32.26161733482779	20.482178473106742
48-49	20.229175976310824	26.510786690378417	32.27520950856568	20.984827824745082
50-51	20.263258782851416	26.305016405298552	32.051019087713016	21.380705724137012
52-53	20.36632993179961	26.68660606640374	31.61787657365631	21.329187428140337
54-55	20.66308343642286	27.42950133775515	31.246012826920982	20.661402398901014
56-57	20.07908756136688	26.857333740994527	31.420409796253256	21.643168901385337
58-59	19.912023158929788	26.147094520678003	32.09734781391338	21.84353450647883
60-61	20.662197690110993	26.8597794407809	31.216735284794762	21.261287584313344
62-63	20.363191481590686	26.618033603327095	31.574007937995685	21.44476697708653
64-65	20.209316784683853	26.31596387877143	32.04390623883215	21.43081309771257
66-67	20.165361598338233	26.526475222245388	31.401530671726015	21.906632507690365
68-69	20.9284269342641	26.69381631225753	31.14318882238271	21.23456793109566
70-71	21.003907959180363	26.284490333866025	31.201499715918917	21.5101019910347
72-73	20.505688976801707	26.43742173892747	31.656257656796065	21.400631627474755
74-75	20.46913318067885	26.426020180481235	31.37579391983003	21.729052719009886
76-77	20.56938626388019	26.553859460589074	30.906293756359755	21.970460519170985
78-79	20.80164126161102	26.793361181923142	30.966513604637985	21.43848395182785
80-81	20.353841029857062	26.43465229166319	31.212529318045984	21.998977360433766
82-83	20.113200122050344	26.459249612908604	31.413027822084654	22.014522442956398
84-85	20.327473724883614	26.40051615992976	30.99634709215595	22.275663023030674
86-87	20.56925323366226	26.52060190610534	30.755751924282105	22.154392935950305
88-89	20.025292672526284	26.41721323945754	31.015305973129347	22.542188114886834
90-91	19.832797947174427	26.216216673814017	30.885444293112496	23.065541085899063
92-93	20.062475926027826	26.549414454481525	30.643704002462275	22.74440561702838
94-95	19.909858724327282	26.213471413862088	30.98879667879177	22.88787318301886
96-97	19.766848003614566	26.28773400248574	30.95068927430431	22.994728719595383
98-99	19.756703191341664	26.160852888490492	30.87201351401687	23.210430406150966
100	19.912741619799593	26.348784874569102	30.528798941947393	23.20967456368391
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5134.0
1	3207.5
2	10174.0
3	17467.5
4	10072.5
5	4299.5
6	7280.0
7	16223.0
8	24700.0
9	25395.5
10	16862.5
11	8281.0
12	6366.5
13	6459.0
14	6854.0
15	7290.0
16	7752.5
17	8465.0
18	9208.0
19	9848.5
20	10748.5
21	12076.5
22	14362.5
23	18168.5
24	22620.0
25	28038.5
26	35499.0
27	44921.5
28	57549.5
29	69205.5
30	76963.0
31	85223.5
32	94286.5
33	101953.5
34	109277.5
35	116215.0
36	120239.0
37	122978.0
38	125286.0
39	126285.5
40	128300.5
41	127802.0
42	123850.0
43	121913.0
44	121542.0
45	120767.5
46	120787.0
47	122054.5
48	123524.5
49	125375.5
50	127340.5
51	124249.0
52	115721.0
53	108594.0
54	104602.5
55	101446.5
56	94892.0
57	86298.5
58	80295.0
59	74309.0
60	63957.5
61	52429.5
62	42600.5
63	32817.0
64	24558.5
65	18248.5
66	13119.0
67	9420.0
68	6682.0
69	4678.0
70	3273.0
71	2301.0
72	1739.0
73	1294.0
74	983.5
75	694.0
76	413.5
77	308.0
78	247.0
79	148.0
80	71.5
81	29.0
82	16.0
83	7.0
84	3.0
85	2.0
86	2.0
87	2.5
88	1.5
89	1.0
90	1.5
91	2.0
92	1.0
93	0.5
94	3.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04849556126537376
2	0.0
3	1.2093656175903681E-4
4	0.07050601550551847
5	0.005466332591508464
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.012045281551200066
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	4.8374624703614725E-4
26-27	0.0
28-29	3.628096852771104E-4
30-31	0.003749033414530141
32-33	1.5721753028674783E-4
34-35	0.0014633323972843453
36-37	0.0036764714774747183
38-39	0.0012577402422939827
40-41	0.0010521480873036202
42-43	0.001777767457857841
44-45	1.209365617590368E-5
46-47	4.111843099807251E-4
48-49	5.200272155638583E-4
50-51	0.001547987990515671
52-53	9.312115255445835E-4
54-55	0.0011488973367108496
56-57	0.005974266150896418
58-59	0.008622776853419323
60-61	0.0010763353996554276
62-63	0.0025638551092915804
64-65	0.0010521480873036202
66-67	1.2093656175903681E-4
68-69	7.135257143783171E-4
70-71	3.8699699762891776E-4
72-73	0.0
74-75	1.9349849881445888E-4
76-77	0.0
78-79	4.474652785084362E-4
80-81	0.0
82-83	9.553988378963907E-4
84-85	5.200272155638583E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	1.5721753028674783E-4
94-95	0.0
96-97	3.5071602910120674E-4
98-99	6.04682808795184E-4
100	2.6606043586988097E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4134399.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.55356270761256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.19950766983638	44.78398595597193
2	16.14431168359127	19.229025564399894
3	4.505887287783994	8.05024923439435
4	1.688595240698727	4.02247450218911
5	0.7764314227256476	2.3119628710726343
6	0.4193955730357303	1.4985900334847078
7	0.25958280563867775	1.08213566253947
8	0.17876176635126625	0.8516720049698976
9	0.12751538413023042	0.6834595882486484
>10	0.6188060995970763	6.641453765387934
>50	0.04744361953373551	1.9559146850081346
>100	0.03079111825308321	3.3937657526729956
>500	0.00199378243287691	0.8114815183316718
>1k	6.510309275644542E-4	0.6210178749043305
>5k	1.2206829891832942E-4	0.5775214223737499
>10k+	2.0344716486388236E-4	3.4852895640505372
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40928	0.9899383199347717	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34627	0.8375340648060334	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27531	0.6659008963576084	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25899	0.6264272025994588	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14845	0.35906065186258024	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9035	0.2185323670985795	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7985	0.19313568912918178	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6813	0.16478815905286354	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	3.628096852771104E-5	0.0
12-13	0.0	0.0	0.0	6.04682808795184E-5	0.0
14-15	0.0	0.0	0.0	1.209365617590368E-4	0.0
16-17	0.0	0.0	0.0	1.9349849881445888E-4	0.0
18-19	0.0	0.0	0.0	2.0559215499036255E-4	0.0
20-21	0.0	0.0	0.0	3.3862237292530306E-4	0.0
22-23	0.0	0.0	0.0	9.07024213192776E-4	0.0
24-25	0.0	0.0	0.0	0.002491293172236158	0.0
26-27	0.0	0.0	0.0	0.00443837181655665	0.0
28-29	0.0	0.0	0.0	0.00916699138133499	0.0
30-31	0.0	0.0	0.0	0.019773127847602517	0.0
32-33	0.0	0.0	0.0	0.04115471196660023	0.0
34-35	0.0	0.0	0.0	0.0739406138594751	0.0
36-37	0.0	0.0	0.0	0.12073096960404645	0.0
38-39	0.0	0.0	0.0	0.18695583082329498	0.0
40-41	0.0	0.0	0.0	0.2671367712695364	0.0
42-43	0.0	0.0	0.0	0.34851498367719225	0.0
44-45	0.0	0.0	0.0	0.4455544808326434	0.0
46-47	0.0	0.0	0.0	0.5624638550851042	0.0
48-49	0.0	0.0	0.0	0.69007611505324	0.0
50-51	0.0	0.0	0.0	0.8246059463539925	0.0
52-53	0.0	0.0	0.0	0.959885584337651	0.0
54-55	0.0	0.0	0.0	1.115724437820346	0.0
56-57	0.0	0.0	0.0	1.2953877939695708	0.0
58-59	0.0	0.0	0.0	1.496239235738979	0.0
60-61	0.0	0.0	0.0	1.7017225478237585	0.0
62-63	0.0	0.0	0.0	1.9115716697880392	0.0
64-65	0.0	0.0	0.0	2.148099397276363	0.0
66-67	0.0	0.0	0.0	2.4264590814771383	0.0
68-69	0.0	0.0	0.0	2.727832993380658	0.0
70-71	0.0	0.0	0.0	3.0547608007838623	0.0
72-73	0.0	0.0	0.0	3.417425362186862	0.0
74-75	0.0	0.0	0.0	3.789075510128558	0.0
76-77	0.0	0.0	0.0	4.1750566406387	0.0
78-79	0.0	0.0	0.0	4.607320193334026	0.0
80-81	0.0	0.0	0.0	5.076469397365857	0.0
82-83	0.0	0.0	0.0	5.573192137478748	0.0
84-85	0.0	2.418731235180736E-5	0.0	6.069745566405178	0.0
86-87	0.0	2.418731235180736E-5	0.0	6.6004635740285345	0.0
88	0.0	2.418731235180736E-5	0.0	7.028760407498163	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13180	0.0	56.059734	1
TACCTGG	4435	0.0	47.088436	2
GTATCAA	24320	0.0	42.28192	1
TATAACG	510	0.0	40.57957	2
ATAACGC	500	0.0	39.509743	3
GTACCTG	5510	0.0	38.093426	1
GTGGTAT	5000	0.0	33.601994	1
TCTGTCG	1345	0.0	32.504135	8
ACCTGGG	6370	0.0	32.4153	3
TATCAAC	32620	0.0	31.31849	2
ATCAACG	32605	0.0	31.246342	3
TCAACGC	32970	0.0	30.88616	4
TGGTATC	5340	0.0	30.388046	2
CAACGCA	34250	0.0	29.785755	5
AACGCAG	35560	0.0	28.845026	6
TAACGCA	765	0.0	27.053047	4
GTATAAC	1120	0.0	26.472157	1
CTGTCGC	1665	0.0	25.692425	9
ACGCAGA	39955	0.0	25.566221	7
CGCAGAG	40060	0.0	25.393599	8
>>END_MODULE