FastQCFastQC Report
Wed 25 May 2016
SRR2431437_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431437_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3886813
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA703301.809451599549554No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61900.1592564396589185No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51990.13375997250189292No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87050.033.320141
TACCTGG31800.032.9640272
GTATCAA132050.029.0141371
GTACATG225500.026.5382541
GTACCTG41150.026.5038281
TACATGG223550.025.6956182
ATCAACG148750.025.0598663
TCAACGC148600.024.9266744
ACCTGGG41300.024.3571383
TATCAAC159450.023.7024942
CATGGGT73100.023.535354
ACATGGG232000.023.402193
CAACGCA165000.022.4776025
AACGCAG173800.021.4449216
CGCAGAG177250.020.3646098
CATGGGA105250.020.2317314
ACGCAGA180650.020.1894637
TCTGTCG7850.019.1593768
GCAGAGT186650.018.1051439
CATGGGG77200.017.9622884
Produced by FastQC (version 0.11.5)