FastQCFastQC Report
Wed 25 May 2016
SRR2431437_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431437_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3886813
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT513831.3219828172850097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT435671.1208926181938776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325690.837935861591489No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289160.7439514069753291No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151400.3895222126714097No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115570.2973387194084202No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96950.24943314741408965No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70380.1810738000516104No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40250.10355527780729354No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA174050.053.000731
TATAACG3850.050.079042
GTATCAA307000.039.839441
TACCTGG30350.036.1019022
GCGGTAT2550.033.205541
ATCAACG409600.029.7927283
TATCAAC412750.029.5197872
TCAACGC414250.029.492364
GCGTATC1652.9413059E-928.509811
CAACGCA431900.028.3298955
CGGTATC3000.028.2152632
TAACGCA6850.028.1466144
ACCTGGG38700.027.9479373
AACGCAG445900.027.5662126
GTGGTAT73650.027.400771
TGGTATC75000.026.5850492
GTACCTG42250.026.0535811
ACGCAGA493050.024.7394057
CGCAGAG493050.024.6345378
CATGGGT69850.023.9671544
Produced by FastQC (version 0.11.5)