##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431437_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3886813 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.218905823357076 33.0 31.0 34.0 30.0 34.0 2 32.34883232097865 34.0 31.0 34.0 30.0 34.0 3 32.44192864436751 34.0 31.0 34.0 30.0 34.0 4 36.00185859211647 37.0 35.0 37.0 35.0 37.0 5 35.93496239721335 37.0 35.0 37.0 35.0 37.0 6 35.986634551237735 37.0 35.0 37.0 35.0 37.0 7 35.921671816987335 37.0 35.0 37.0 35.0 37.0 8 35.90850653221547 37.0 35.0 37.0 35.0 37.0 9 37.57738640886505 39.0 37.0 39.0 35.0 39.0 10-11 37.51690536694201 39.0 37.0 39.0 34.5 39.0 12-13 37.494634936128904 39.0 37.0 39.0 34.5 39.0 14-15 38.768162630926675 40.0 38.0 41.0 34.0 41.0 16-17 38.826549026155874 40.0 38.0 41.0 34.5 41.0 18-19 38.94429253478364 40.0 38.5 41.0 35.0 41.0 20-21 38.95520764698482 40.0 38.5 41.0 35.0 41.0 22-23 38.8542759839488 40.0 38.0 41.0 35.0 41.0 24-25 38.77012001863738 40.0 38.0 41.0 35.0 41.0 26-27 38.592191726229174 40.0 38.0 41.0 34.0 41.0 28-29 38.468725534261615 40.0 38.0 41.0 34.0 41.0 30-31 38.20598881397176 40.0 37.5 41.0 34.0 41.0 32-33 37.89619168712258 40.0 36.5 41.0 33.5 41.0 34-35 37.68848012497642 40.0 36.0 41.0 33.0 41.0 36-37 37.50500500024056 40.0 36.0 41.0 33.0 41.0 38-39 37.37781249059319 40.0 36.0 41.0 33.0 41.0 40-41 37.21952355310122 40.0 36.0 41.0 33.0 41.0 42-43 37.03357249242502 40.0 35.0 41.0 31.5 41.0 44-45 36.736139608465855 40.0 35.0 41.0 31.0 41.0 46-47 36.292167130242696 40.0 35.0 41.0 30.0 41.0 48-49 35.91594218708232 40.0 35.0 41.0 28.0 41.0 50-51 35.73197720085839 40.0 35.0 41.0 27.0 41.0 52-53 35.57997593401072 39.5 35.0 41.0 27.0 41.0 54-55 35.41190237348697 39.0 35.0 41.0 27.0 41.0 56-57 35.17396244686843 39.0 35.0 41.0 26.0 41.0 58-59 34.98619563122795 39.0 35.0 41.0 26.0 41.0 60-61 34.766746046182305 38.0 35.0 41.0 26.0 41.0 62-63 34.53476807348334 38.0 35.0 41.0 26.0 41.0 64-65 34.28855285808707 37.0 34.5 40.0 25.0 41.0 66-67 34.01950531193551 37.0 34.0 40.0 24.5 41.0 68-69 33.72986351543025 36.0 34.0 40.0 23.0 41.0 70-71 33.40843899616472 36.0 34.0 39.0 23.0 41.0 72-73 33.09216818509149 35.0 34.0 39.0 22.5 41.0 74-75 32.794798463419774 35.0 34.0 38.5 22.0 40.5 76-77 32.45699844577035 35.0 33.0 37.0 20.5 40.0 78-79 32.15833241784465 35.0 33.0 37.0 20.0 39.0 80-81 31.8657481077685 35.0 33.0 36.5 20.0 39.0 82-83 31.62512397689315 35.0 33.0 36.0 19.0 38.5 84-85 31.404511228093554 35.0 33.0 36.0 18.5 37.0 86-87 31.168885408173743 35.0 33.0 35.5 17.5 37.0 88-89 31.016869733635243 35.0 33.0 35.0 14.0 36.5 90-91 30.82653539030563 35.0 33.0 35.0 8.5 36.0 92-93 30.633327613137034 35.0 33.0 35.0 2.0 36.0 94-95 30.46969728155175 35.0 33.0 35.0 2.0 36.0 96-97 30.30323969792218 35.0 33.0 35.0 2.0 36.0 98-99 30.134213300202504 35.0 32.5 35.0 2.0 35.0 100 30.021432211943306 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1799138815704424 1101 2 0.22639547021447015 1101 3 0.2523650541684219 1101 4 0.17952064660978095 1101 5 0.18311002316782776 1101 6 0.18717224985164904 1101 7 0.2075693131559717 1101 8 0.25943718234019997 1101 9 0.3405302567707551 1101 10-11 0.2997072233215974 1101 12-13 0.3390548175821735 1101 14-15 0.4518884132470191 1101 16-17 0.4234902129747411 1101 18-19 0.4182110222004667 1101 20-21 0.33858755239972993 1101 22-23 0.3619170970734942 1101 24-25 0.40774130794061136 1101 26-27 0.40202023861556313 1101 28-29 0.3836187533081059 1101 30-31 0.40074550287254596 1101 32-33 0.4055859384496827 1101 34-35 0.4225002484268572 1101 36-37 0.45626363227013655 1101 38-39 0.4085338024818981 1101 40-41 0.47114110822948874 1101 42-43 0.44417608003238485 1101 44-45 0.4744119831218043 1101 46-47 0.5660329883221706 1101 48-49 0.5214829630806506 1101 50-51 0.46301542403281815 1101 52-53 0.42841033039730547 1101 54-55 0.29293812903398475 1101 56-57 0.4106918860657274 1101 58-59 0.40192442235296966 1101 60-61 0.44190104537315733 1101 62-63 0.428328032259202 1101 64-65 0.38138966204680713 1101 66-67 0.30353724342955957 1101 68-69 0.30852645927983957 1101 70-71 0.337263522820642 1101 72-73 0.37583059846065225 1101 74-75 0.3700634597088879 1101 76-77 0.3611826313379396 1101 78-79 0.36527707876145854 1101 80-81 0.3579872261097634 1101 82-83 0.33165079516352947 1101 84-85 0.34331554711511103 1101 86-87 0.3013366799396522 1101 88-89 0.2933290619588824 1101 90-91 0.32867601036095095 1101 92-93 0.36284135824988084 1101 94-95 0.32928041646009376 1101 96-97 0.3821885856471532 1101 98-99 0.44956367685743714 1101 100 0.4833318852393873 1102 1 0.11765936509186758 1102 2 0.20326514270465879 1102 3 0.22297686881803713 1102 4 0.16932536562551803 1102 5 0.14067282531144798 1102 6 0.16976971229115634 1102 7 0.18727506566417418 1102 8 0.2458733240585218 1102 9 0.24154766607369993 1102 10-11 0.29863131189204495 1102 12-13 0.2911459610908622 1102 14-15 0.39709221062055633 1102 16-17 0.3719244354149325 1102 18-19 0.36773685483788654 1102 20-21 0.3548601169565728 1102 22-23 0.3457492114690055 1102 24-25 0.298387428625972 1102 26-27 0.3676151514890904 1102 28-29 0.35262328168760604 1102 30-31 0.3374936764339438 1102 32-33 0.33340814020355225 1102 34-35 0.4047251534316274 1102 36-37 0.41115232385580214 1102 38-39 0.39732359078563206 1102 40-41 0.4960904170470357 1102 42-43 0.5759709249076437 1102 44-45 0.6020088760389299 1102 46-47 0.6208603270941424 1102 48-49 0.5847963545200301 1102 50-51 0.42475920316933014 1102 52-53 0.4495640123841227 1102 54-55 0.4748946573780586 1102 56-57 0.49473979778213106 1102 58-59 0.4516180795833975 1102 60-61 0.4449106728846104 1102 62-63 0.409439493325209 1102 64-65 0.4297708192695495 1102 66-67 0.42365735606952626 1102 68-69 0.40642625015564704 1102 70-71 0.419057146851479 1102 72-73 0.39846224023869325 1102 74-75 0.41370798659261254 1102 76-77 0.4008324445702698 1102 78-79 0.4316463944698583 1102 80-81 0.4049988234552302 1102 82-83 0.3790258781778775 1102 84-85 0.37913815147086183 1102 86-87 0.4156972975774522 1102 88-89 0.42289960210350586 1102 90-91 0.4553957250240863 1102 92-93 0.474863766680091 1102 94-95 0.47146618494445036 1102 96-97 0.44068070255939773 1102 98-99 0.5117280445848813 1102 100 0.510593125895312 1103 1 0.1530280420867669 1103 2 0.19649239611006664 1103 3 0.18536925035049023 1103 4 0.17628823553591388 1103 5 0.10007139151638711 1103 6 0.14152227584409616 1103 7 0.12446597176960239 1103 8 0.134822515845606 1103 9 0.2528161206185757 1103 10-11 0.26661702892703687 1103 12-13 0.2379290008705297 1103 14-15 0.3174531850984721 1103 16-17 0.33686157810288364 1103 18-19 0.28402600904743736 1103 20-21 0.28080489509115125 1103 22-23 0.30293667644212263 1103 24-25 0.2922254754646474 1103 26-27 0.30228764119213025 1103 28-29 0.3191888141099284 1103 30-31 0.3362525726975534 1103 32-33 0.2930853202998449 1103 34-35 0.40185404362567567 1103 36-37 0.3901927615412504 1103 38-39 0.38657615509387 1103 40-41 0.4602446341562043 1103 42-43 0.5191378452029838 1103 44-45 0.4984334368413741 1103 46-47 0.5765816098781755 1103 48-49 0.5333507164044917 1103 50-51 0.5255715249003998 1103 52-53 0.551023729232405 1103 54-55 0.5164266772804211 1103 56-57 0.5585210644269907 1103 58-59 0.5803990577048737 1103 60-61 0.562228435622913 1103 62-63 0.6072674258330935 1103 64-65 0.6318903634207302 1103 66-67 0.5792127756716496 1103 68-69 0.5530737745796799 1103 70-71 0.6263374680157696 1103 72-73 0.59076392270768 1103 74-75 0.5831040995934842 1103 76-77 0.5987234141508395 1103 78-79 0.6001337343355999 1103 80-81 0.5757910573120455 1103 82-83 0.5796029702669898 1103 84-85 0.5307619490005564 1103 86-87 0.5184659438166648 1103 88-89 0.4975746658581137 1103 90-91 0.5011910178330261 1103 92-93 0.5268446534408575 1103 94-95 0.5267644259789996 1103 96-97 0.563593402029511 1103 98-99 0.6586805427593276 1103 100 0.6566170772638671 1104 1 0.08300469978772895 1104 2 0.16855841539288718 1104 3 0.12031023317462086 1104 4 0.13352622762616306 1104 5 0.09755942097587678 1104 6 0.15812057064122342 1104 7 0.14255629723019325 1104 8 0.1974365794423747 1104 9 0.26559389499694674 1104 10-11 0.3673429100325407 1104 12-13 0.3543819999815341 1104 14-15 0.4440028430642968 1104 16-17 0.37780138026299426 1104 18-19 0.36438759177828217 1104 20-21 0.3176839210258464 1104 22-23 0.3576398976560782 1104 24-25 0.3649310987996017 1104 26-27 0.3399082680804497 1104 28-29 0.3765263846634923 1104 30-31 0.3834976365341163 1104 32-33 0.3743931533879845 1104 34-35 0.37283926871670303 1104 36-37 0.38186539417436904 1104 38-39 0.34475816849901264 1104 40-41 0.41245716999934956 1104 42-43 0.32660046066519755 1104 44-45 0.36246498201649047 1104 46-47 0.4111024983366036 1104 48-49 0.41314336883966263 1104 50-51 0.32958655476519993 1104 52-53 0.25840413236564785 1104 54-55 0.2803927352291211 1104 56-57 0.35290421263324134 1104 58-59 0.4269356065702894 1104 60-61 0.3954611706696127 1104 62-63 0.32150551436949115 1104 64-65 0.29675412813642055 1104 66-67 0.34820640235862754 1104 68-69 0.3088221544446341 1104 70-71 0.2506147556470353 1104 72-73 0.3192481126780393 1104 74-75 0.33845333517761844 1104 76-77 0.31424891308483893 1104 78-79 0.30062201117509346 1104 80-81 0.21391026125544244 1104 82-83 0.204670883216032 1104 84-85 0.2267251531922092 1104 86-87 0.2790445566063582 1104 88-89 0.2777156899413846 1104 90-91 0.2577044254502887 1104 92-93 0.30326426936373707 1104 94-95 0.2971393159473479 1104 96-97 0.29884850500690163 1104 98-99 0.31086952068364937 1104 100 0.365672554778655 1105 1 0.07108423958810306 1105 2 0.14647527701995244 1105 3 0.1291777110302661 1105 4 0.09297924710193683 1105 5 0.05197846973196363 1105 6 0.10286693623636722 1105 7 0.1174291719483449 1105 8 0.12684655376865805 1105 9 0.19838085812924078 1105 10-11 0.22811945121317478 1105 12-13 0.13089615160853185 1105 14-15 0.26926887949825584 1105 16-17 0.24158162816067374 1105 18-19 0.21520214408073457 1105 20-21 0.17579428357062454 1105 22-23 0.2500183274112189 1105 24-25 0.27663445588173374 1105 26-27 0.24793004409302455 1105 28-29 0.26773767095121315 1105 30-31 0.2794943234812237 1105 32-33 0.3270981305308922 1105 34-35 0.29044385893919866 1105 36-37 0.2535302547797187 1105 38-39 0.25818501962788787 1105 40-41 0.1898372461183797 1105 42-43 0.21306459112587817 1105 44-45 0.19232536574751435 1105 46-47 0.16134502636813153 1105 48-49 0.20955429841853146 1105 50-51 0.16359814674967765 1105 52-53 0.19513772876991453 1105 54-55 0.2553956594571787 1105 56-57 0.2857823399453565 1105 58-59 0.24551188596667117 1105 60-61 0.25102776081530465 1105 62-63 0.324781759995993 1105 64-65 0.29792571238824195 1105 66-67 0.3346070979221025 1105 68-69 0.27117850752332373 1105 70-71 0.2572490313461273 1105 72-73 0.2862612402345306 1105 74-75 0.23206193337883008 1105 76-77 0.24464546744606253 1105 78-79 0.22540774830929422 1105 80-81 0.2513759209685489 1105 82-83 0.2392725501213917 1105 84-85 0.20121259059743224 1105 86-87 0.18188037889971653 1105 88-89 0.2055148124253101 1105 90-91 0.17239539140601323 1105 92-93 0.18530636065550254 1105 94-95 0.21739474132624537 1105 96-97 0.2178066662420477 1105 98-99 0.2316165636692169 1105 100 0.22936973723474452 1106 1 0.16350434935632308 1106 2 0.23051301052165485 1106 3 0.15320361132182114 1106 4 0.153877350547603 1106 5 0.15057121445956057 1106 6 0.13675802564169715 1106 7 0.13918124630245643 1106 8 0.19307021906103472 1106 9 0.22429750753785527 1106 10-11 0.20595994563451114 1106 12-13 0.22495162594689333 1106 14-15 0.3002505996380336 1106 16-17 0.27644770741669333 1106 18-19 0.2656486307348729 1106 20-21 0.23370961784141286 1106 22-23 0.21750477697708703 1106 24-25 0.2303507064785677 1106 26-27 0.3082691606527277 1106 28-29 0.21591768568787018 1106 30-31 0.3037309819604488 1106 32-33 0.275671056441233 1106 34-35 0.32229916706716466 1106 36-37 0.31183377243950616 1106 38-39 0.3283175527600548 1106 40-41 0.2542468449971338 1106 42-43 0.2530813041954474 1106 44-45 0.26663841091755813 1106 46-47 0.3327967208524498 1106 48-49 0.26373572094076536 1106 50-51 0.18849092915085208 1106 52-53 0.170889131582193 1106 54-55 0.1921416321632421 1106 56-57 0.23252233039045933 1106 58-59 0.2145640459456004 1106 60-61 0.1758244611638986 1106 62-63 0.15138040847807588 1106 64-65 0.2074924786825676 1106 66-67 0.24444678505666673 1106 68-69 0.3413828833419714 1106 70-71 0.2610563009169269 1106 72-73 0.3426592923199081 1106 74-75 0.3559527873769639 1106 76-77 0.36515361254926404 1106 78-79 0.35113622347022755 1106 80-81 0.29771016086746016 1106 82-83 0.29513369702134895 1106 84-85 0.27408792626663825 1106 86-87 0.26773619111895286 1106 88-89 0.20890390453099528 1106 90-91 0.32870132604400837 1106 92-93 0.2784910808706762 1106 94-95 0.3381966965023011 1106 96-97 0.33099350708609876 1106 98-99 0.37641978415196675 1106 100 0.4517104923295783 1107 1 0.1280297711615006 1107 2 0.1912646621464944 1107 3 0.14134364818785627 1107 4 0.14756899577549376 1107 5 0.130184957145147 1107 6 0.14113377745164257 1107 7 0.06767564826466099 1107 8 0.06146074005621216 1107 9 0.12615635707695816 1107 10-11 0.19044130750766186 1107 12-13 0.1300980707864099 1107 14-15 0.25166584350641585 1107 16-17 0.22043388603232472 1107 18-19 0.1763479291701202 1107 20-21 0.17747005334595656 1107 22-23 0.18706485719859955 1107 24-25 0.20442582681262422 1107 26-27 0.2659212472911179 1107 28-29 0.2963491200869939 1107 30-31 0.2506189112681625 1107 32-33 0.2900686283063507 1107 34-35 0.28928979614864403 1107 36-37 0.2980138111983024 1107 38-39 0.24486642623254795 1107 40-41 0.24331689367154752 1107 42-43 0.34598464522907335 1107 44-45 0.35376866985148325 1107 46-47 0.4323282638715469 1107 48-49 0.33572251292302013 1107 50-51 0.2889711624863338 1107 52-53 0.3319788108348476 1107 54-55 0.30753745465267457 1107 56-57 0.37829367627836064 1107 58-59 0.3776240409575422 1107 60-61 0.3542538441291754 1107 62-63 0.36551046853036695 1107 64-65 0.39138594785787006 1107 66-67 0.3927518260835683 1107 68-69 0.38121560218203854 1107 70-71 0.39046331699146464 1107 72-73 0.4339854557152165 1107 74-75 0.408344935003349 1107 76-77 0.4293792176172957 1107 78-79 0.4174751683017419 1107 80-81 0.40084060765381224 1107 82-83 0.4378284258040779 1107 84-85 0.41764933782105373 1107 86-87 0.3814157255580284 1107 88-89 0.39077564757734606 1107 90-91 0.41727783516961736 1107 92-93 0.44622728483571805 1107 94-95 0.4802179316533284 1107 96-97 0.4674494363027364 1107 98-99 0.5173838157496 1107 100 0.5393126948992446 1108 1 0.048378111408545976 1108 2 0.09185241700837565 1108 3 0.08027251650314327 1108 4 0.07616316231266751 1108 5 0.04157364984574485 1108 6 0.05836531912667198 1108 7 0.11118304844876548 1108 8 0.08669578704189718 1108 9 0.1592265191113711 1108 10-11 0.07139963786853087 1108 12-13 0.07934819746166255 1108 14-15 0.17251031054035337 1108 16-17 0.08079951306028477 1108 18-19 0.101373218421827 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-0.3039111444629299 2215 14-15 -0.4608199919127003 2215 16-17 -0.44501984661550864 2215 18-19 -0.4627280556857869 2215 20-21 -0.44618320023894853 2215 22-23 -0.47857274804221817 2215 24-25 -0.5354956353140352 2215 26-27 -0.41071588947042414 2215 28-29 -0.45042587667005307 2215 30-31 -0.5274790881159603 2215 32-33 -0.44304267008191545 2215 34-35 -0.40379763851754547 2215 36-37 -0.4072774271029971 2215 38-39 -0.428942199824931 2215 40-41 -0.4090766903611467 2215 42-43 -0.3786416076215957 2215 44-45 -0.30816273617654844 2215 46-47 -0.44637316164998 2215 48-49 -0.48251427384148116 2215 50-51 -0.2991088331118732 2215 52-53 -0.3087771714287939 2215 54-55 -0.28276815942918887 2215 56-57 -0.2531801066092143 2215 58-59 -0.3468774909896766 2215 60-61 -0.4120118315945831 2215 62-63 -0.24763664016975895 2215 64-65 -0.22943742735384376 2215 66-67 -0.27304478384341735 2215 68-69 -0.3510766859631218 2215 70-71 -0.27626393917075376 2215 72-73 -0.32530125433881096 2215 74-75 -0.3321266851785225 2215 76-77 -0.3087948104933531 2215 78-79 -0.28250797517632265 2215 80-81 -0.25259702079530655 2215 82-83 -0.15896830374135362 2215 84-85 -0.1692575921899504 2215 86-87 -0.2307507249292442 2215 88-89 -0.27094958233046285 2215 90-91 -0.28842891153164274 2215 92-93 -0.2834382632529504 2215 94-95 -0.3952307295721873 2215 96-97 -0.5236838201184959 2215 98-99 -0.4860070962784526 2215 100 -0.48279220469122563 2216 1 -0.2871898955348655 2216 2 -0.33712607913818005 2216 3 -0.34453575194228137 2216 4 -0.20917804859691103 2216 5 -0.3323545543881963 2216 6 -0.37999577441593146 2216 7 -0.3877374103968947 2216 8 -0.4254910281827904 2216 9 -0.7891602050275566 2216 10-11 -0.6245896396372217 2216 12-13 -0.5595649541633847 2216 14-15 -0.711657435317079 2216 16-17 -0.5365261072678607 2216 18-19 -0.6443627516924835 2216 20-21 -0.7782884092719513 2216 22-23 -0.745106354556917 2216 24-25 -0.6623238868791148 2216 26-27 -0.7690607847978299 2216 28-29 -0.7340872937377156 2216 30-31 -0.7850302296855389 2216 32-33 -0.7609793143152146 2216 34-35 -0.9352512944373501 2216 36-37 -0.9997087102942217 2216 38-39 -1.0215030558460256 2216 40-41 -1.1190687701611566 2216 42-43 -1.1313636362557347 2216 44-45 -1.12193182345883 2216 46-47 -1.265817501072675 2216 48-49 -0.9925280283295379 2216 50-51 -1.0120525700938288 2216 52-53 -1.1741093585727924 2216 54-55 -1.0778166193434942 2216 56-57 -1.3541557700339908 2216 58-59 -1.40345228859357 2216 60-61 -1.2668169959711406 2216 62-63 -1.4049133185078873 2216 64-65 -1.4709223211818951 2216 66-67 -1.3503037313901274 2216 68-69 -1.243412898668673 2216 70-71 -1.3311969858271198 2216 72-73 -1.1611571620064822 2216 74-75 -1.075010792733373 2216 76-77 -1.113266169550851 2216 78-79 -1.0237792007613749 2216 80-81 -1.0134535117837586 2216 82-83 -1.0731770474606606 2216 84-85 -1.152806757814382 2216 86-87 -1.232227741766259 2216 88-89 -1.1775606538453793 2216 90-91 -1.281417827788598 2216 92-93 -1.2334962820665574 2216 94-95 -1.2730946455860916 2216 96-97 -1.4406750309865828 2216 98-99 -1.4959734289471598 2216 100 -1.5607857583484197 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 451.0 3 1238.0 4 852.0 5 584.0 6 981.0 7 2164.0 8 3620.0 9 5482.0 10 7135.0 11 9091.0 12 12028.0 13 16798.0 14 17821.0 15 22923.0 16 30115.0 17 43805.0 18 49050.0 19 27731.0 20 17884.0 21 16020.0 22 15584.0 23 16308.0 24 17930.0 25 20934.0 26 25403.0 27 31851.0 28 39534.0 29 49517.0 30 61483.0 31 77090.0 32 96087.0 33 123044.0 34 167753.0 35 269480.0 36 388681.0 37 719389.0 38 996449.0 39 481493.0 40 3030.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.504362251562725 21.013401423668 14.868772096004282 22.613464228764993 2 14.417647568843678 22.290781676401718 39.607462463462994 23.684108291291604 3 16.59043013970079 28.734768667443483 31.624569987653107 23.050231205202625 4 11.108682432310662 18.882522832584392 38.205091368956126 31.803703366148817 5 11.213716107632244 38.42051827773178 34.96499034384952 15.400775270786452 6 26.861724502825325 39.32828772570227 18.52561468740585 15.28437308406656 7 23.791857236249854 34.997361591617604 23.34691686993946 17.863864302193083 8 25.301525954554542 33.71348197096181 21.18571179009641 19.799280284387237 9 25.73036058076373 15.577955512652656 23.021020049073623 35.67066385750999 10-11 23.05877179484936 27.278060619284812 28.85887389568732 20.804293690178508 12-13 25.014143978627217 25.808393663394664 29.427824286890058 19.74963807108806 14-15 22.094026648567862 26.43314715680945 29.46087192772073 22.01195426690196 16-17 18.97473585685753 29.501303510099408 30.061595451080358 21.462365181962703 18-19 18.734256060170633 26.32843411813226 35.26055922937378 19.676750592323327 20-21 20.46651073771751 25.258560162271763 35.39231241636786 18.882616683642873 22-23 19.97721526608046 25.665808980262234 35.39559273883256 18.961383014824744 24-25 19.534842795224392 25.59671616204977 35.519201110118146 19.349239932607688 26-27 19.29993802120143 25.56406752781778 35.65401782900284 19.481976621977953 28-29 19.30629761552991 25.621485457239658 35.779889685503214 19.292327241727218 30-31 19.747880133265305 25.793099154214534 35.31462432104702 19.14439639147314 32-33 19.207036104157392 25.65084220736285 35.72231470022975 19.419806988250006 34-35 19.55942962217602 25.46458397064699 35.370978942084626 19.605007465092363 36-37 19.84257762574847 25.710944630728942 34.85238999119428 19.594087752328306 38-39 19.731807814467818 25.695855036257658 34.97758418633491 19.594752962939616 40-41 19.622412382486274 25.679752906004456 34.83783491733517 19.8599997941741 42-43 19.670806552120002 25.779972017695023 34.699254542750715 19.849966887434256 44-45 19.89120905626397 25.806724661917702 34.26149319011401 20.04057309170432 46-47 20.055552103702748 26.216646257854947 33.507461083361214 20.220340555081087 48-49 20.20323436272084 26.15876042810095 32.96523213800784 20.67277307117036 50-51 20.219244729372402 26.298137069442934 32.5921833201368 20.890434881047867 52-53 20.350194897908384 26.699090262025482 32.16330696488107 20.787407875185064 54-55 20.39123720258904 26.939755285394245 32.033493084557946 20.635514427458773 56-57 20.277652382109597 26.526374750697045 32.12844999075667 21.067522876436687 58-59 20.390032714570932 26.001966553664236 32.49697124233676 21.11102948942807 60-61 20.35382565434102 26.482277200134916 32.252539574603404 20.911357570920657 62-63 20.201326697914112 26.253889046476882 32.57730977360717 20.967474482001844 64-65 20.15443135318724 26.250615709602986 32.56330343751523 21.03164949969454 66-67 20.172192576382024 26.22664184083044 32.29942232848471 21.30174325430282 68-69 20.546824267235568 26.34517241485772 32.167730784390606 20.940272533516108 70-71 20.524914692100424 26.31536766849259 31.986480920180416 21.173236719226573 72-73 20.43734802780581 26.23618630482094 32.13266241519723 21.19380325217601 74-75 20.32735353208945 26.380535488384165 31.885838427274628 21.40627255225176 76-77 20.438197052443737 26.333592586008127 31.59522981939188 21.63298054215626 78-79 20.510819653994908 26.42106569877348 31.810265147933674 21.257849499297944 80-81 20.121562318537066 26.459209640391755 31.841858612698886 21.577369428372293 82-83 20.11517748349278 26.479809528906163 31.862382885182193 21.542630102418865 84-85 20.113455915875182 26.265000787280833 31.643132286850147 21.978411009993838 86-87 20.267015675824897 26.436131607051845 31.380658138171295 21.91619457895196 88-89 20.06228496199843 26.341002255575457 31.47245571114432 22.124257071281793 90-91 19.926865532249685 26.31092620097751 31.34785491352427 22.414353353248536 92-93 19.963082736837855 26.469811473424343 31.38744357664963 22.17966221308817 94-95 19.840265019181523 26.263766741543783 31.406347565473308 22.48962067380139 96-97 19.806038111868276 26.513133122937166 31.173284309216758 22.507544455977797 98-99 19.6882671418146 26.42979507894626 31.26579147134158 22.61614630789756 100 19.77727150518615 26.388888531553633 31.101949035130488 22.73189092812973 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5255.0 1 3281.5 2 10826.5 3 18483.0 4 10515.0 5 4651.5 6 8841.5 7 20432.0 8 31870.5 9 33405.0 10 21577.0 11 9541.5 12 6867.5 13 6825.5 14 7334.5 15 7854.5 16 8186.0 17 8714.5 18 9487.0 19 10189.5 20 11141.0 21 12540.5 22 14606.0 23 17936.0 24 22298.5 25 27829.0 26 34810.5 27 43137.0 28 53910.0 29 64439.5 30 71895.0 31 79470.5 32 87154.5 33 93331.5 34 99522.5 35 105495.5 36 109836.5 37 113137.0 38 115664.0 39 116708.0 40 118681.5 41 119737.0 42 118605.0 43 119913.0 44 119992.0 45 117946.5 46 117114.5 47 117422.5 48 119359.0 49 122281.5 50 123902.0 51 117034.5 52 106149.0 53 98370.0 54 92502.0 55 89058.0 56 81209.0 57 69510.5 58 64168.0 59 64097.5 60 57469.5 61 46367.0 62 37175.0 63 28309.0 64 21458.5 65 16436.5 66 12493.0 67 9279.0 68 6799.0 69 4874.5 70 3395.0 71 2474.0 72 1915.5 73 1390.0 74 1056.0 75 838.5 76 524.0 77 412.0 78 329.5 79 190.5 80 105.5 81 42.0 82 17.5 83 6.5 84 4.5 85 4.0 86 2.0 87 2.0 88 2.0 89 1.0 90 1.0 91 1.5 92 1.5 93 1.0 94 2.0 95 2.5 96 1.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04574441837052619 2 0.0 3 1.543681159860276E-4 4 0.0687195396331133 5 0.004399491305601788 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011809160872931114 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.016963769545898E-4 26-27 0.0 28-29 4.2451231896157595E-4 30-31 0.0033446425130305986 32-33 7.71840579930138E-5 34-35 0.001440769082536258 36-37 0.003486146619351124 38-39 0.0012349449278882209 40-41 8.490246379231519E-4 42-43 0.0018266893725013271 44-45 1.2864009665502301E-5 46-47 2.958722223065529E-4 48-49 5.016963769545898E-4 50-51 0.0015565451695257787 52-53 8.876166669196587E-4 54-55 0.0010419847829056866 56-57 0.005827396378472543 58-59 0.008194374156924967 60-61 0.0010291207732401842 62-63 0.00245702584611094 64-65 9.648007249126727E-4 66-67 5.1456038662009205E-5 68-69 6.432004832751152E-4 70-71 2.5728019331004606E-4 72-73 0.0 74-75 1.543681159860276E-4 76-77 0.0 78-79 5.274243962855944E-4 80-81 0.0 82-83 7.461125605991335E-4 84-85 4.3737632862707825E-4 86-87 0.0 88-89 0.0 90-91 0.0 92-93 3.473282609685622E-4 94-95 0.0 96-97 4.759683576235852E-4 98-99 6.046084542786082E-4 100 4.631043479580829E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3886813.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.39747682189682 #Duplication Level Percentage of deduplicated Percentage of total 1 71.53138362205644 36.05001248130799 2 16.764153397388323 16.897420645672014 3 5.640099719142754 8.527403846060512 4 2.3301244450246963 4.697295708410692 5 1.1416778859003978 2.8768842396368726 6 0.6363327780636111 1.9241739860084432 7 0.3946865680794945 1.3923845016690224 8 0.27826261234070715 1.1218986844672998 9 0.18516055331449277 0.8398462224597065 >10 0.9481359866489671 8.78071077643534 >50 0.08253481919086682 2.8969137402874012 >100 0.06121935051722072 6.028920041192795 >500 0.003982003801981824 1.37791996653421 >1k 0.0018378478881465026 1.445972054857592 >5k 1.0210266045258338E-4 0.4305163679281877 >10k+ 3.063079813577502E-4 4.711726737071945 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 51383 1.3219828172850097 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43567 1.1208926181938776 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32569 0.837935861591489 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28916 0.7439514069753291 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15140 0.3895222126714097 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11557 0.2973387194084202 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9695 0.24943314741408965 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7038 0.1810738000516104 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4025 0.10355527780729354 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.5728019331004606E-5 0.0 5 0.0 0.0 0.0 2.5728019331004606E-5 0.0 6 0.0 0.0 0.0 2.5728019331004606E-5 0.0 7 0.0 0.0 0.0 2.5728019331004606E-5 0.0 8 0.0 0.0 0.0 2.5728019331004606E-5 0.0 9 0.0 0.0 0.0 5.145603866200921E-5 0.0 10-11 0.0 0.0 0.0 6.432004832751152E-5 0.0 12-13 0.0 0.0 0.0 1.1577608698952073E-4 0.0 14-15 0.0 0.0 0.0 1.2864009665502303E-4 0.0 16-17 0.0 0.0 0.0 1.4150410632052533E-4 0.0 18-19 0.0 0.0 0.0 1.6723212565152994E-4 0.0 20-21 0.0 0.0 0.0 3.601922706340645E-4 0.0 22-23 0.0 0.0 0.0 0.001067712802236691 0.0 24-25 0.0 0.0 0.0 0.002495617875107447 0.0 26-27 0.0 0.0 0.0 0.00457958744091882 0.0 28-29 0.0 0.0 0.0 0.00917203889150314 0.0 30-31 0.0 0.0 0.0 0.020428047348817657 0.0 32-33 0.0 0.0 0.0 0.039466781653761066 0.0 34-35 0.0 0.0 0.0 0.0698258444643465 0.0 36-37 0.0 0.0 0.0 0.11482415027427355 0.0 38-39 0.0 0.0 0.0 0.17588960415641297 0.0 40-41 0.0 0.0 0.0 0.24507224813748435 0.0 42-43 0.0 0.0 0.0 0.315772845259085 0.0 44-45 0.0 0.0 0.0 0.402463406394905 0.0 46-47 0.0 0.0 0.0 0.5092732786475707 0.0 48-49 0.0 0.0 0.0 0.6247277653954538 0.0 50-51 0.0 0.0 0.0 0.7463183847537815 0.0 52-53 0.0 0.0 0.0 0.8668026992808762 0.0 54-55 0.0 0.0 0.0 1.0039201783054652 0.0 56-57 0.0 0.0 0.0 1.1713581281116432 0.0 58-59 0.0 0.0 0.0 1.359815869711252 0.0 60-61 0.0 0.0 0.0 1.5605716045510807 0.0 62-63 0.0 0.0 0.0 1.7651736782808949 0.0 64-65 0.0 0.0 0.0 1.986293140421214 0.0 66-67 0.0 0.0 0.0 2.2456058472584095 0.0 68-69 0.0 0.0 0.0 2.540127348550085 0.0 70-71 0.0 0.0 0.0 2.861122981733364 0.0 72-73 0.0 0.0 0.0 3.198237219027517 0.0 74-75 0.0 0.0 0.0 3.5457584401410616 0.0 76-77 0.0 0.0 0.0 3.913772028651752 0.0 78-79 0.0 0.0 0.0 4.336869306550122 0.0 80-81 0.0 0.0 0.0 4.801002775281445 0.0 82-83 0.0 0.0 0.0 5.290568391121466 0.0 84-85 0.0 0.0 0.0 5.77534859536592 0.0 86-87 0.0 0.0 0.0 6.286332787299003 0.0 88 0.0 0.0 0.0 6.697569448285781 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17405 0.0 53.00073 1 TATAACG 385 0.0 50.07904 2 GTATCAA 30700 0.0 39.83944 1 TACCTGG 3035 0.0 36.101902 2 GCGGTAT 255 0.0 33.20554 1 ATCAACG 40960 0.0 29.792728 3 TATCAAC 41275 0.0 29.519787 2 TCAACGC 41425 0.0 29.49236 4 GCGTATC 165 2.9413059E-9 28.50981 1 CAACGCA 43190 0.0 28.329895 5 CGGTATC 300 0.0 28.215263 2 TAACGCA 685 0.0 28.146614 4 ACCTGGG 3870 0.0 27.947937 3 AACGCAG 44590 0.0 27.566212 6 GTGGTAT 7365 0.0 27.40077 1 TGGTATC 7500 0.0 26.585049 2 GTACCTG 4225 0.0 26.053581 1 ACGCAGA 49305 0.0 24.739405 7 CGCAGAG 49305 0.0 24.634537 8 CATGGGT 6985 0.0 23.967154 4 >>END_MODULE