FastQCFastQC Report
Wed 25 May 2016
SRR2431436_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431436_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4323732
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA598831.3849840831947955No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68500.15842795066854282No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA63650.14721078919785038No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA63630.14716453286188874No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55770.12898579282897277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85950.042.0068441
GTATCAA136000.033.080931
TCAACGC159900.027.3691124
ATCAACG160900.027.1113663
TATCAAC174450.025.4908712
GTACATG281850.025.436451
TACATGG280800.024.2904072
CATGGGT65750.023.807184
ACATGGG290550.022.4234183
CAACGCA201500.021.8117735
ACGCAGA200200.021.4109027
CGCAGAG199000.021.2565928
AACGCAG206250.021.1018856
ATGCGGG13800.020.0944988
GTCGGGA14550.019.707492
GCAGAGT205550.019.298759
CATGGGA130050.019.229184
GTGGTAT48500.019.0954191
AATGCGG14550.019.0586997
CATGGGG122950.018.6956184
Produced by FastQC (version 0.11.5)