##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431436_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4323732
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.530604579562286	31.0	30.0	34.0	23.0	34.0
2	29.613957803120083	31.0	30.0	34.0	23.0	34.0
3	29.71611908416155	31.0	30.0	34.0	23.0	34.0
4	32.86104272882778	37.0	35.0	37.0	22.0	37.0
5	33.1086554393288	37.0	35.0	37.0	26.0	37.0
6	33.03485414914708	37.0	35.0	37.0	25.0	37.0
7	32.956146218128225	37.0	35.0	37.0	24.0	37.0
8	32.838263102338445	37.0	35.0	37.0	22.0	37.0
9	34.016348145537236	39.0	35.0	39.0	17.0	39.0
10-11	34.05325318498001	39.0	35.0	39.0	17.5	39.0
12-13	33.99929112165139	39.0	35.0	39.0	17.5	39.0
14-15	35.13118655365319	40.0	36.0	41.0	17.5	41.0
16-17	35.17802664457464	40.0	36.0	41.0	19.0	41.0
18-19	35.157068130032116	40.0	35.0	41.0	20.0	41.0
20-21	35.25286396104106	40.0	35.0	41.0	21.5	41.0
22-23	35.23864591977486	40.0	35.0	41.0	23.5	41.0
24-25	35.28491486058803	40.0	35.0	41.0	24.5	41.0
26-27	35.28428658390483	40.0	35.0	41.0	25.0	41.0
28-29	35.1878405044531	40.0	35.0	41.0	25.0	41.0
30-31	35.16347162590096	40.0	35.0	41.0	25.0	41.0
32-33	35.09955693368599	40.0	35.0	41.0	25.0	41.0
34-35	34.977779034408236	40.0	34.5	41.0	24.5	41.0
36-37	34.834486966352216	40.0	34.0	41.0	24.0	41.0
38-39	34.7107573966194	40.0	34.0	41.0	23.5	41.0
40-41	34.594739567577264	39.0	34.0	41.0	23.0	41.0
42-43	34.349137735641335	39.0	33.0	41.0	22.0	41.0
44-45	34.1927737426834	39.0	33.0	41.0	21.0	41.0
46-47	34.03734412771189	39.0	33.0	41.0	20.5	41.0
48-49	33.88164715111853	38.0	33.0	40.5	19.5	41.0
50-51	33.68219688454326	38.0	33.0	40.0	18.0	41.0
52-53	33.536836811347236	38.0	33.0	40.0	18.0	41.0
54-55	33.43959593240284	38.0	33.0	40.0	17.0	41.0
56-57	33.24661750543281	37.5	32.5	40.0	14.5	41.0
58-59	33.010608659371115	37.0	32.0	40.0	11.5	41.0
60-61	32.78208501359474	36.5	32.0	40.0	8.5	41.0
62-63	32.504116004414705	36.0	31.5	40.0	7.5	41.0
64-65	32.344620457512164	36.0	31.0	40.0	4.0	41.0
66-67	32.09093660291619	35.0	31.0	39.0	2.0	41.0
68-69	31.832131246802533	35.0	31.0	39.0	2.0	41.0
70-71	31.564114057022962	35.0	31.0	39.0	2.0	41.0
72-73	31.274982353207832	35.0	31.0	38.0	2.0	40.5
74-75	30.95714547062584	35.0	30.5	37.0	2.0	40.0
76-77	30.67427167086212	35.0	30.0	37.0	2.0	39.0
78-79	30.374539171252984	35.0	30.0	36.0	2.0	39.0
80-81	30.11811879182151	35.0	30.0	36.0	2.0	39.0
82-83	29.856935860039428	35.0	30.0	36.0	2.0	37.5
84-85	29.60052369110759	35.0	30.0	35.0	2.0	37.0
86-87	29.37315714757529	35.0	29.5	35.0	2.0	37.0
88-89	29.14120683243087	34.5	29.0	35.0	2.0	36.0
90-91	28.93909289474926	34.0	29.0	35.0	2.0	36.0
92-93	28.776482446183067	34.0	29.0	35.0	2.0	36.0
94-95	28.558831467815303	34.0	29.0	35.0	2.0	35.5
96-97	28.496892268068418	34.0	29.0	35.0	2.0	35.0
98-99	28.363945776472733	34.0	29.0	35.0	2.0	35.0
100	28.233121756852643	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3912040542223636
1101	2	0.49598135183384073
1101	3	0.47028114734817095
1101	4	0.40756494150563327
1101	5	0.3952204326909907
1101	6	0.3664040563239368
1101	7	0.34953394623047984
1101	8	0.5101553041009979
1101	9	0.539700319472395
1101	10-11	0.6484072288390905
1101	12-13	0.5099759852197963
1101	14-15	0.638150181499185
1101	16-17	0.5707771279829075
1101	18-19	0.570165801967093
1101	20-21	0.5475300898428515
1101	22-23	0.5163511061814816
1101	24-25	0.5645202343872029
1101	26-27	0.6336005961502735
1101	28-29	0.6400899145450154
1101	30-31	0.7063037545156803
1101	32-33	0.663083476465836
1101	34-35	0.6874992698144666
1101	36-37	0.7140241084158916
1101	38-39	0.7633777649902882
1101	40-41	0.7530400571072988
1101	42-43	0.7765291965604249
1101	44-45	0.6943351868277077
1101	46-47	0.5551425716335245
1101	48-49	0.6822488043855586
1101	50-51	0.6264607225967183
1101	52-53	0.5966126951943735
1101	54-55	0.7261419832352516
1101	56-57	0.6684397633626133
1101	58-59	0.6762747210507811
1101	60-61	0.662417922724984
1101	62-63	0.5834816723471974
1101	64-65	0.5818173429413562
1101	66-67	0.6357804684765185
1101	68-69	0.60161342787368
1101	70-71	0.6458818144629284
1101	72-73	0.6442144347373251
1101	74-75	0.6677156996080171
1101	76-77	0.6149934485863255
1101	78-79	0.641848660412581
1101	80-81	0.6386356300901781
1101	82-83	0.5717999940820739
1101	84-85	0.5360033689466768
1101	86-87	0.4969668958010125
1101	88-89	0.5103811747434044
1101	90-91	0.4915847878224149
1101	92-93	0.4314679221007651
1101	94-95	0.44904440237750975
1101	96-97	0.45445210737775454
1101	98-99	0.47814385529611414
1101	100	0.434831865421927
1102	1	0.45981148832172636
1102	2	0.5723620314423066
1102	3	0.47452691198308017
1102	4	0.4983308848850214
1102	5	0.47593045844443793
1102	6	0.4433887897952147
1102	7	0.5065686921481856
1102	8	0.5162652830620118
1102	9	0.6537152384693101
1102	10-11	0.7064483698703157
1102	12-13	0.6477753187246549
1102	14-15	0.7593416378511506
1102	16-17	0.6480800104280107
1102	18-19	0.6364202707441464
1102	20-21	0.6604792482942514
1102	22-23	0.6508903756251598
1102	24-25	0.6639389549727284
1102	26-27	0.7360425023237269
1102	28-29	0.7832689755224322
1102	30-31	0.7500028806679637
1102	32-33	0.7882076932262052
1102	34-35	0.8331594195810865
1102	36-37	0.7143439906468529
1102	38-39	0.6846859658385753
1102	40-41	0.7218324324927181
1102	42-43	0.7332072051481262
1102	44-45	0.6519492643090317
1102	46-47	0.6018161045661756
1102	48-49	0.5874308360006069
1102	50-51	0.6063634412022552
1102	52-53	0.6513788056267344
1102	54-55	0.7014601542186938
1102	56-57	0.6770457701384913
1102	58-59	0.697494602819539
1102	60-61	0.6762559999738897
1102	62-63	0.6116270718205925
1102	64-65	0.567834167310572
1102	66-67	0.6462741298176624
1102	68-69	0.7043923837772041
1102	70-71	0.6567699981393211
1102	72-73	0.6988120340606621
1102	74-75	0.6450519596331183
1102	76-77	0.5915291088934609
1102	78-79	0.5247151991740431
1102	80-81	0.5583841746494116
1102	82-83	0.5209349502136114
1102	84-85	0.5121348226882994
1102	86-87	0.5296171956049704
1102	88-89	0.5369418544795259
1102	90-91	0.49267098979716195
1102	92-93	0.4606632627094456
1102	94-95	0.4798793216869619
1102	96-97	0.4982682421417408
1102	98-99	0.5078867125710289
1102	100	0.540554442157898
1103	1	0.26285983533025714
1103	2	0.3841421134672913
1103	3	0.35660210955395044
1103	4	0.32473629292459805
1103	5	0.36154687362081717
1103	6	0.38442641531246835
1103	7	0.37721550220540223
1103	8	0.4818159336850911
1103	9	0.5561180868836928
1103	10-11	0.5461214750026571
1103	12-13	0.5500422358363934
1103	14-15	0.6553603396459948
1103	16-17	0.5908778995338366
1103	18-19	0.5568914768052551
1103	20-21	0.6128346049579392
1103	22-23	0.5771118099359072
1103	24-25	0.5986285752905403
1103	26-27	0.5600736302445242
1103	28-29	0.6193010998985287
1103	30-31	0.5690948103797453
1103	32-33	0.6750395106782392
1103	34-35	0.6125496518062832
1103	36-37	0.6381139299779193
1103	38-39	0.6352339173498436
1103	40-41	0.6361808756662981
1103	42-43	0.6353811222594956
1103	44-45	0.5513334487273696
1103	46-47	0.5069199894961756
1103	48-49	0.49831750853651613
1103	50-51	0.4021731188801354
1103	52-53	0.39344742128898247
1103	54-55	0.4883625891185659
1103	56-57	0.4880571385932626
1103	58-59	0.48041753856571034
1103	60-61	0.580523686855102
1103	62-63	0.4924474777796277
1103	64-65	0.5484156255537869
1103	66-67	0.5439842874974445
1103	68-69	0.46955280062464055
1103	70-71	0.47174307572185015
1103	72-73	0.47390257339704434
1103	74-75	0.46398213510452635
1103	76-77	0.4711727801538821
1103	78-79	0.43262091294543836
1103	80-81	0.33576183500677814
1103	82-83	0.40300170052949014
1103	84-85	0.4098697741307511
1103	86-87	0.31957943477124573
1103	88-89	0.4010007813541563
1103	90-91	0.46009167822598585
1103	92-93	0.4552617109487045
1103	94-95	0.40912214663936197
1103	96-97	0.4569806660917166
1103	98-99	0.48335569695061054
1103	100	0.5333149842385652
1104	1	0.190544776360543
1104	2	0.3808542601940097
1104	3	0.23642760795273077
1104	4	0.3045906766069635
1104	5	0.2338959833099068
1104	6	0.3013466194365293
1104	7	0.27649575616667477
1104	8	0.2744573158692276
1104	9	0.3118275930906904
1104	10-11	0.3687014286158501
1104	12-13	0.35843258367652453
1104	14-15	0.35092798417940685
1104	16-17	0.3740443278168186
1104	18-19	0.21376369056645927
1104	20-21	0.20433647064925964
1104	22-23	0.2843164935977711
1104	24-25	0.26628713326497433
1104	26-27	0.35463501506685446
1104	28-29	0.41844227000805034
1104	30-31	0.30301655796102267
1104	32-33	0.3687009974000617
1104	34-35	0.34402838009631154
1104	36-37	0.30064372859026633
1104	38-39	0.32955956502006956
1104	40-41	0.3306288214756421
1104	42-43	0.22433908246302536
1104	44-45	0.306818858898005
1104	46-47	0.253635372760435
1104	48-49	0.2022763553038729
1104	50-51	0.2262676157738568
1104	52-53	0.2540955034835477
1104	54-55	0.26238949121865573
1104	56-57	0.3279049252092392
1104	58-59	0.3688893200349028
1104	60-61	0.27702228201546575
1104	62-63	0.32124895523492825
1104	64-65	0.39532693069738656
1104	66-67	0.3918883956495307
1104	68-69	0.34731312758239596
1104	70-71	0.4005493825860924
1104	72-73	0.38865261723030997
1104	74-75	0.40327363841070607
1104	76-77	0.33616468646564
1104	78-79	0.3873351493422952
1104	80-81	0.3206158421567551
1104	82-83	0.3158721821635275
1104	84-85	0.2920983072321697
1104	86-87	0.2717178040580208
1104	88-89	0.28798060367202183
1104	90-91	0.2635071748094404
1104	92-93	0.27260469934893194
1104	94-95	0.30024558359685827
1104	96-97	0.3086885329045401
1104	98-99	0.2541780361501118
1104	100	0.23113594919250247
1105	1	0.2774020534724997
1105	2	0.307649921328057
1105	3	0.32719357668867843
1105	4	0.32404696033314195
1105	5	0.2896501307002026
1105	6	0.40358377789023336
1105	7	0.4196675156459051
1105	8	0.40908917805231937
1105	9	0.45044526367472315
1105	10-11	0.5586786966417563
1105	12-13	0.4354456163112559
1105	14-15	0.5221178628576482
1105	16-17	0.5077256823504968
1105	18-19	0.5193418233764291
1105	20-21	0.5213900550921196
1105	22-23	0.45600595924739906
1105	24-25	0.5359283199903331
1105	26-27	0.568465731747736
1105	28-29	0.6144160777611773
1105	30-31	0.6411624986578417
1105	32-33	0.5766992015120067
1105	34-35	0.5670200896279098
1105	36-37	0.568252296100745
1105	38-39	0.5372478939578471
1105	40-41	0.4833814667357217
1105	42-43	0.5143157646327623
1105	44-45	0.48660074589952274
1105	46-47	0.43876807534541484
1105	48-49	0.38258096179546897
1105	50-51	0.39937191152532137
1105	52-53	0.4179534800776068
1105	54-55	0.5542538497728984
1105	56-57	0.47175763563514295
1105	58-59	0.44964205118703404
1105	60-61	0.47086231607256934
1105	62-63	0.5008323551685407
1105	64-65	0.5761835620600593
1105	66-67	0.5614726135188377
1105	68-69	0.530574019673395
1105	70-71	0.6159761299078035
1105	72-73	0.5752181530512281
1105	74-75	0.5427426611143424
1105	76-77	0.5584102339775363
1105	78-79	0.5699103005466206
1105	80-81	0.5948903969418886
1105	82-83	0.5588181657460289
1105	84-85	0.5673992669576258
1105	86-87	0.6236186043752774
1105	88-89	0.6452808607220781
1105	90-91	0.5885778741982648
1105	92-93	0.5990231445354084
1105	94-95	0.6612313788977566
1105	96-97	0.6822344435240595
1105	98-99	0.7220744249954798
1105	100	0.6464137406947366
1106	1	0.2441812602653286
1106	2	0.2632835247154368
1106	3	0.2698966131884113
1106	4	0.3273197819473168
1106	5	0.29729793423707207
1106	6	0.2564132473604701
1106	7	0.37635869168257585
1106	8	0.3853587078295533
1106	9	0.5017556782226578
1106	10-11	0.43919047262869526
1106	12-13	0.39268783607435864
1106	14-15	0.4897621576662914
1106	16-17	0.4048855213132754
1106	18-19	0.38560922649717355
1106	20-21	0.48713343664788056
1106	22-23	0.46981633099264286
1106	24-25	0.42721435412277486
1106	26-27	0.4668922026541793
1106	28-29	0.5912571601309793
1106	30-31	0.5350048919193924
1106	32-33	0.5566802167861127
1106	34-35	0.44637320574795325
1106	36-37	0.48207116281504625
1106	38-39	0.49715896197727005
1106	40-41	0.5294843413939105
1106	42-43	0.5470253297274965
1106	44-45	0.48721663115653513
1106	46-47	0.46363611372373725
1106	48-49	0.471096754460234
1106	50-51	0.5318790763323094
1106	52-53	0.6165642174999348
1106	54-55	0.5895573310855298
1106	56-57	0.6163668164236071
1106	58-59	0.6892962753468268
1106	60-61	0.6918504849703311
1106	62-63	0.6952978973956405
1106	64-65	0.6814586682817776
1106	66-67	0.7078380162346463
1106	68-69	0.7060461661925217
1106	70-71	0.6663187299701256
1106	72-73	0.6517265327990778
1106	74-75	0.6957311161891049
1106	76-77	0.6609133647670546
1106	78-79	0.6053576353447916
1106	80-81	0.5541742870781867
1106	82-83	0.5807106335716945
1106	84-85	0.6380107466333378
1106	86-87	0.6304574187486196
1106	88-89	0.6574311435732163
1106	90-91	0.6771787986927791
1106	92-93	0.6552663603081328
1106	94-95	0.6651174993932791
1106	96-97	0.6760402923903968
1106	98-99	0.6763603809538026
1106	100	0.6694396239037772
1107	1	0.17245560327208054
1107	2	0.15883887250886985
1107	3	0.27187855529445315
1107	4	0.2070532343137188
1107	5	0.13338204354658956
1107	6	0.18249917580742903
1107	7	0.20825397221345554
1107	8	0.2727262385404998
1107	9	0.2496911719061501
1107	10-11	0.22886165274262282
1107	12-13	0.30577390307396257
1107	14-15	0.3687224117425387
1107	16-17	0.28088587292039335
1107	18-19	0.34409657372507496
1107	20-21	0.3104810831556222
1107	22-23	0.2935024441701657
1107	24-25	0.3206665468911396
1107	26-27	0.3183511374370269
1107	28-29	0.45715996600716835
1107	30-31	0.40011790291365656
1107	32-33	0.3924530485829507
1107	34-35	0.39802893812699836
1107	36-37	0.34704913629499146
1107	38-39	0.3420077821247389
1107	40-41	0.35651479166924105
1107	42-43	0.34689020256563907
1107	44-45	0.38746364789262344
1107	46-47	0.3506511054592991
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2216	3	-0.7934331994123305
2216	4	-0.6597963107741407
2216	5	-0.9177028645286143
2216	6	-0.859212645941092
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2216	9	-1.1719386272360026
2216	10-11	-1.3077430355002235
2216	12-13	-1.3195049348213175
2216	14-15	-1.453800559266469
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2216	56-57	-1.593030284981495
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2216	64-65	-1.5780831146598544
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2216	80-81	-1.5006097842346833
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2216	88-89	-1.4830432237110713
2216	90-91	-1.673997837719078
2216	92-93	-1.588662406201081
2216	94-95	-1.537391028825553
2216	96-97	-1.6249133476323117
2216	98-99	-1.9324980658558992
2216	100	-1.8539293115572875
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	158264.0
3	35155.0
4	25059.0
5	21277.0
6	14817.0
7	13727.0
8	14826.0
9	15230.0
10	14797.0
11	15135.0
12	16560.0
13	17941.0
14	18809.0
15	18934.0
16	18566.0
17	17638.0
18	16586.0
19	15263.0
20	14802.0
21	16645.0
22	21477.0
23	38035.0
24	59433.0
25	63858.0
26	69432.0
27	54614.0
28	56691.0
29	64296.0
30	72658.0
31	84145.0
32	105623.0
33	138316.0
34	190992.0
35	303331.0
36	500493.0
37	790868.0
38	853503.0
39	354949.0
40	987.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.65793834598014	22.408521493412586	14.048926092713272	21.884614067894
2	15.380117473066173	23.15767731406073	37.57204442641326	23.890160786459834
3	18.357772753294665	29.495949251435544	28.30636496637387	23.839913028895918
4	12.310509946767688	20.281779209189988	36.25542812758916	31.15228271645317
5	12.653247687492916	41.866312918813016	32.09433687312496	13.386102520569112
6	28.19069023049936	39.84160729870311	17.845849084666632	14.121853386130901
7	25.4958526667376	36.780207528577975	20.479823096459615	17.244116708224812
8	24.28903279408591	38.6685991093524	18.952942044529607	18.089426052032085
9	25.192440244066038	20.80774016866277	20.111532074145007	33.88828751312618
10-11	23.39222811811277	30.946598250538432	25.78213968052556	19.87903395082324
12-13	23.527619203580127	29.041016805434026	25.958042967343236	21.473321023642608
14-15	21.596187609383545	30.410397984075644	23.814153127769462	24.17926127877135
16-17	20.80414691704709	33.34492116458398	24.909662331522533	20.9412695868464
18-19	20.948685395308903	32.018320019558374	26.40720976938184	20.625784815750876
20-21	23.04323580303538	30.300114930644938	25.75001555461033	20.90663371170935
22-23	22.713599953370636	30.879451531367497	25.57981392663647	20.827134588625395
24-25	21.710344703165173	30.887826651418127	25.9370850775424	21.464743567874304
26-27	21.62739903840705	31.047050145821014	26.148613688463218	21.17693712730872
28-29	21.2473558633993	31.08057733404873	26.51907809110253	21.152988711449446
30-31	21.697452598708285	31.1836089617067	25.910963714568652	21.207974725016363
32-33	21.296258282530346	30.878290822271165	26.304801742576732	21.520649152621758
34-35	21.824616621859274	30.497486356846316	26.401955542788137	21.275941478506272
36-37	21.377911308881476	30.82258595942726	26.284223336591943	21.51527939509932
38-39	21.447198996916597	30.659997793438553	26.19342245775325	21.699380751891596
40-41	21.706795840522283	30.32804279797584	26.37023154173072	21.594929819771156
42-43	21.541023389168537	30.684836834627156	26.619319962521082	21.15481981368323
44-45	21.624279293615224	30.469562413728024	26.231179756569624	21.674978536087128
46-47	21.641643463767625	30.50194358492076	26.33836279974298	21.51805015156863
48-49	21.325219960442336	30.532247546380454	26.23742023792191	21.9051122552553
50-51	21.348463517073842	30.695959309297955	26.272492259242014	21.68308491438619
52-53	21.61097072460446	30.616749385549635	26.111258044405105	21.661021845440796
54-55	21.494040509547304	30.45557281513876	26.217560027422078	21.83282664789186
56-57	21.419947654683742	30.51831160913277	26.161209326680847	21.900531409502648
58-59	21.894518578824844	30.229426601181192	26.3273703310085	21.548684488985465
60-61	21.460194619373933	30.693680623697905	26.084755239741252	21.761369517186907
62-63	21.213586748298084	30.56618235742344	26.471485843735305	21.748745050543178
64-65	21.847876518961197	30.393020584644802	26.06017928934658	21.698923607047416
66-67	21.373041280909394	30.45043990697996	26.069433822792178	22.10708498931847
68-69	21.62113591088457	30.687528174113883	26.41201315304676	21.279322761954784
70-71	21.61859604487105	30.71391233953972	26.105932693419682	21.56155892216954
72-73	21.827229423662484	30.644614030685112	26.24569368777603	21.282462857876375
74-75	21.708352510980408	30.504104209214063	26.048822627387764	21.73872065241777
76-77	21.612557300280354	30.275514330693294	25.64766273140848	22.46426563761787
78-79	21.807734647154557	30.19756930121313	26.280807429690938	21.713888621941376
80-81	21.168697965894435	30.72918262819655	26.298202181935594	21.80391722397342
82-83	21.396505215624877	30.90843763002635	26.257995861360435	21.437061292988343
84-85	21.471235060645565	30.156174054270213	26.386704337386934	21.985886547697287
86-87	21.92233497659882	30.57113315006812	25.90561837669547	21.600913496637585
88-89	21.73221899577787	30.425832833176635	25.951286472409272	21.890661698636222
90-91	21.582415833779912	30.27665262672434	25.845270291054923	22.295661248440833
92-93	21.52144939150929	30.340577862352212	26.2141857233803	21.923787022758194
94-95	21.567707746081858	30.20213882408042	26.34033264814638	21.88982078169134
96-97	21.634595758769535	30.774980864077534	25.73088006509278	21.859543312060147
98-99	21.3084959910039	30.753407247717263	26.08287532920673	21.85522143207211
100	21.467500537745078	30.644692745182844	25.798857898839167	22.08894881823291
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	53191.0
1	40615.5
2	24804.5
3	17744.5
4	11088.0
5	6930.0
6	5083.5
7	4612.5
8	4735.5
9	4748.0
10	4010.5
11	3132.5
12	2884.5
13	2815.0
14	2839.5
15	2928.5
16	3118.0
17	3518.0
18	4067.0
19	4852.0
20	5962.5
21	7273.5
22	9376.5
23	13195.5
24	18183.5
25	24089.5
26	32024.0
27	41145.0
28	51084.0
29	61853.5
30	71236.0
31	78817.5
32	85842.0
33	93124.5
34	99715.5
35	105850.5
36	110555.5
37	113988.5
38	117448.0
39	119706.0
40	122002.5
41	123191.0
42	121996.0
43	122306.5
44	122618.0
45	125192.5
46	131939.0
47	133384.0
48	136839.5
49	147142.0
50	153756.5
51	148091.5
52	140794.0
53	135234.5
54	132466.5
55	133970.5
56	118676.0
57	98687.5
58	88206.5
59	82391.0
60	72044.5
61	58371.0
62	49871.5
63	40196.5
64	30747.0
65	24362.5
66	19383.5
67	15310.5
68	12148.5
69	9409.5
70	7035.0
71	5403.0
72	4375.0
73	3465.5
74	2867.0
75	2340.5
76	1622.5
77	1216.5
78	905.0
79	562.5
80	358.0
81	232.5
82	174.0
83	138.5
84	101.5
85	84.5
86	78.5
87	67.5
88	52.0
89	36.0
90	30.0
91	25.5
92	26.5
93	26.5
94	18.0
95	18.5
96	22.5
97	16.5
98	15.5
99	25.5
100	41.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01480202750771787
2	0.008279884137129682
3	0.010569572767229791
4	0.009667574215978234
5	0.011610340326366205
6	0.012789876893387474
7	0.010916495286941929
8	0.00834926864107211
9	0.007215988410012461
10-11	0.006649348294482637
12-13	0.007146603906070034
14-15	0.00822206371717766
16-17	0.0053426068035669185
18-19	0.00612896451491443
20-21	0.005897682835106339
22-23	0.006383374362703331
24-25	0.00597863142303917
26-27	0.006499015202607377
28-29	0.016733229534115435
30-31	0.00572422157525027
32-33	0.0057704779112118885
34-35	0.005238530047653278
36-37	0.007204424326022057
38-39	0.006082708178952812
40-41	0.005932375087077553
42-43	0.0054351194754901555
44-45	0.004533120924238598
46-47	0.00642963069866495
48-49	0.0039433526407279635
50-51	0.0043712237483729335
52-53	0.0035270456170733984
54-55	0.004810658940008308
56-57	0.003145430845390047
58-59	0.002937277333562765
60-61	0.003804583632843109
62-63	0.0035039174490925896
64-65	0.0050419406198163996
66-67	0.003411404777169353
68-69	0.003954916724718368
70-71	0.005365734971547728
72-73	0.003168559013370856
74-75	0.0028216364936587187
76-77	0.0029488414175531693
78-79	0.0037120709609198724
80-81	0.004290275160440102
82-83	0.002058406950292016
84-85	0.00292571324957236
86-87	0.0029025850815915513
88-89	0.002012150614330398
90-91	0.002659739317793055
92-93	0.002879456913610742
94-95	0.0021856118741864668
96-97	0.0013298696588965274
98-99	0.001873381606445543
100	0.0028216364936587187
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4323732.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.91559105310882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.44386617925753	31.19112294454346
2	14.474751136155097	13.002840052541293
3	6.105564164659789	8.22705069505125
4	3.215742951713608	5.77747981404342
5	1.9128167223477044	4.295764683025875
6	1.2328413318118092	3.3224278255817556
7	0.7979761228190105	2.5089098441878814
8	0.5722666153144991	2.0562954613450226
9	0.4061495877450925	1.6418203910592584
>10	1.7133024845179603	12.663139108053773
>50	0.06749021477811831	2.0820063889798837
>100	0.04399459404390248	4.06113474447567
>500	0.006722368651462609	2.1532674866616337
>1k	0.006256973558103154	5.041640483723397
>5k	2.0684210109431464E-4	0.5842522452321876
>10k+	5.171052527357866E-5	1.3908478314942987
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	59883	1.3849840831947955	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6850	0.15842795066854282	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	6365	0.14721078919785038	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	6363	0.14716453286188874	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5577	0.12898579282897277	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	2.312816798080917E-5	0.0
14-15	0.0	0.0	0.0	2.312816798080917E-5	0.0
16-17	0.0	0.0	0.0	8.09485879328321E-5	0.0
18-19	0.0	0.0	0.0	1.8502534384647336E-4	0.0
20-21	0.0	0.0	0.0	3.35358435721733E-4	0.0
22-23	0.0	0.0	0.0	8.673062992803439E-4	0.0
24-25	0.0	0.0	0.0	0.0018386893544743291	0.0
26-27	0.0	0.0	0.0	0.0033073280212557116	0.0
28-29	0.0	0.0	0.0	0.0065337074545785915	0.0
30-31	0.0	0.0	0.0	0.015125821859449197	0.0
32-33	0.0	0.0	0.0	0.029661875435387765	0.0
34-35	0.0	0.0	0.0	0.05156425051321405	0.0
36-37	0.0	0.0	0.0	0.08615242572851417	0.0
38-39	0.0	0.0	0.0	0.13347265741724973	0.0
40-41	0.0	0.0	0.0	0.18310570590406622	0.0
42-43	0.0	0.0	0.0	0.23253060087905542	0.0
44-45	0.0	0.0	0.0	0.2932304777446891	0.0
46-47	0.0	0.0	0.0	0.3679575884906835	0.0
48-49	0.0	0.0	0.0	0.4468940258091852	0.0
50-51	0.0	0.0	0.0	0.5297622516844245	0.0
52-53	0.0	0.0	0.0	0.6103292248455732	0.0
54-55	0.0	0.0	0.0	0.699557696915535	0.0
56-57	0.0	0.0	0.0	0.8084682399371654	0.0
58-59	0.0	0.0	0.0	0.9310012738994924	0.0
60-61	0.0	0.0	0.0	1.062866523642076	0.0
62-63	0.0	0.0	0.0	1.1975765380462988	0.0
64-65	0.0	0.0	0.0	1.334923163600334	0.0
66-67	0.0	0.0	0.0	1.4973985436655186	0.0
68-69	0.0	2.312816798080917E-5	0.0	1.6855693183573819	0.0
70-71	0.0	2.312816798080917E-5	0.0	1.889340042352301	0.0
72-73	0.0	2.312816798080917E-5	0.0	2.099725422389732	0.0
74-75	0.0	2.312816798080917E-5	0.0	2.3147711282752956	0.0
76-77	0.0	2.312816798080917E-5	0.0	2.5455092961358385	0.0
78-79	0.0	3.469225197121376E-5	0.0	2.81224645745851	0.0
80-81	0.0	4.625633596161834E-5	0.0	3.105349730279305	0.0
82-83	0.0	4.625633596161834E-5	0.0	3.411774827857046	0.0
84-85	0.0	4.625633596161834E-5	0.0	3.715586442452955	0.0
86-87	0.0	4.625633596161834E-5	0.0	4.042988788389289	0.0
88	0.0	4.625633596161834E-5	0.0	4.305007803443877	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8595	0.0	42.006844	1
GTATCAA	13600	0.0	33.08093	1
TCAACGC	15990	0.0	27.369112	4
ATCAACG	16090	0.0	27.111366	3
TATCAAC	17445	0.0	25.490871	2
GTACATG	28185	0.0	25.43645	1
TACATGG	28080	0.0	24.290407	2
CATGGGT	6575	0.0	23.80718	4
ACATGGG	29055	0.0	22.423418	3
CAACGCA	20150	0.0	21.811773	5
ACGCAGA	20020	0.0	21.410902	7
CGCAGAG	19900	0.0	21.256592	8
AACGCAG	20625	0.0	21.101885	6
ATGCGGG	1380	0.0	20.094498	8
GTCGGGA	1455	0.0	19.70749	2
GCAGAGT	20555	0.0	19.29875	9
CATGGGA	13005	0.0	19.22918	4
GTGGTAT	4850	0.0	19.095419	1
AATGCGG	1455	0.0	19.058699	7
CATGGGG	12295	0.0	18.695618	4
>>END_MODULE