FastQCFastQC Report
Wed 25 May 2016
SRR2431436_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431436_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4323732
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT631491.4605206798201182No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT537151.2423295430891645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401340.9282258937417952No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136240.3150981605705442No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124920.2889170744162681No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93780.2168959593240284No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84340.19506296875014456No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68430.15826605349267717No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA56170.12991091954820513No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49660.11485448219269835No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG48630.112472280890675No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT45600.10546444599248983No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG45430.10507126713681605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA186050.058.122381
GTATCAA342900.042.555661
GTGGTAT69000.033.3491
TATCAAC463850.031.380862
ATCAACG463750.031.347063
TGGTATC73500.031.0338842
TCAACGC470950.030.8874344
CAACGCA506950.028.6426665
AACGCAG516900.028.14496
ACGCAGA555850.025.9612967
CGCAGAG557900.025.8153488
GCAGAGT584000.024.275279
GAGTACT424550.022.7089912-13
GTACATG266250.022.286841
AGTACTT429650.022.14410412-13
TCGATTA11350.021.9736921
CATGGGT61900.021.957554
GCGTATC1303.1358624E-421.7185571
CGATTAT11600.021.4881782
TACATGG270150.021.4828532
Produced by FastQC (version 0.11.5)