##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431436_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4323732
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.253340632583146	33.0	31.0	34.0	30.0	34.0
2	32.38332232432538	34.0	31.0	34.0	31.0	34.0
3	32.47675572861593	34.0	31.0	34.0	30.0	34.0
4	36.035228825468366	37.0	35.0	37.0	35.0	37.0
5	35.954066764545075	37.0	35.0	37.0	35.0	37.0
6	36.001661758869425	37.0	35.0	37.0	35.0	37.0
7	35.93773295847199	37.0	35.0	37.0	35.0	37.0
8	35.925703767023485	37.0	35.0	37.0	35.0	37.0
9	37.593125799656406	39.0	37.0	39.0	35.0	39.0
10-11	37.53692909273748	39.0	37.0	39.0	35.0	39.0
12-13	37.497204613977	39.0	37.0	39.0	35.0	39.0
14-15	38.77208624401327	40.0	38.0	41.0	34.0	41.0
16-17	38.83716104513415	40.0	38.0	41.0	35.0	41.0
18-19	38.96077578351294	40.5	38.5	41.0	35.0	41.0
20-21	38.99938444380919	41.0	39.0	41.0	35.0	41.0
22-23	38.93939055427117	41.0	38.5	41.0	35.0	41.0
24-25	38.86123307365027	41.0	38.0	41.0	35.0	41.0
26-27	38.688658894677104	40.0	38.0	41.0	34.5	41.0
28-29	38.574877096915344	40.0	38.0	41.0	34.0	41.0
30-31	38.31378910626283	40.0	37.5	41.0	34.0	41.0
32-33	38.02174406276799	40.0	37.0	41.0	33.5	41.0
34-35	37.838723815444624	40.0	36.0	41.0	33.0	41.0
36-37	37.66096430121016	40.0	36.0	41.0	33.0	41.0
38-39	37.52845238789084	40.0	36.0	41.0	33.0	41.0
40-41	37.34536298734519	40.0	36.0	41.0	33.0	41.0
42-43	37.134987205497474	40.0	35.0	41.0	32.5	41.0
44-45	36.791908240381225	40.0	35.0	41.0	31.0	41.0
46-47	36.297935440957026	40.0	35.0	41.0	30.0	41.0
48-49	35.889297023959855	40.0	35.0	41.0	28.5	41.0
50-51	35.67256700924109	39.0	35.0	41.0	27.5	41.0
52-53	35.4893470501872	39.0	35.0	41.0	27.0	41.0
54-55	35.30377842567486	39.0	35.0	41.0	27.0	41.0
56-57	35.037451673693	38.5	35.0	41.0	26.0	41.0
58-59	34.83036437040964	38.0	35.0	41.0	26.0	41.0
60-61	34.60872563331863	37.5	35.0	41.0	26.0	41.0
62-63	34.38023193851978	37.0	34.5	40.0	26.0	41.0
64-65	34.14203054213351	36.5	34.0	40.0	26.0	41.0
66-67	33.89271687051834	36.0	34.0	40.0	25.5	41.0
68-69	33.62696149992645	36.0	34.0	39.0	25.0	41.0
70-71	33.32942305397282	35.0	34.0	39.0	24.0	41.0
72-73	33.0389322233663	35.0	34.0	39.0	23.5	41.0
74-75	32.761034911507004	35.0	33.5	37.5	23.0	40.0
76-77	32.45340529894082	35.0	33.0	37.0	22.0	39.5
78-79	32.17388681814691	35.0	33.0	37.0	21.5	39.0
80-81	31.905244936550183	35.0	33.0	36.0	20.5	39.0
82-83	31.692506959265746	35.0	33.0	36.0	20.0	38.0
84-85	31.493227262929338	35.0	33.0	35.5	20.0	37.0
86-87	31.277800751757972	35.0	33.0	35.0	19.5	37.0
88-89	31.151469031845636	35.0	33.0	35.0	19.5	36.0
90-91	30.974624236654815	35.0	33.0	35.0	17.5	36.0
92-93	30.798056285634726	35.0	33.0	35.0	13.5	36.0
94-95	30.647757423448077	35.0	33.0	35.0	4.5	36.0
96-97	30.49917790926912	35.0	33.0	35.0	2.0	35.5
98-99	30.342859131879592	35.0	33.0	35.0	2.0	35.0
100	30.233304931943053	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17464372461991928
1101	2	0.2442623607608141
1101	3	0.18451205876972665
1101	4	0.17681757907046602
1101	5	0.22462948851073605
1101	6	0.23290938992333565
1101	7	0.24575200140109388
1101	8	0.2467863816984348
1101	9	0.37827606828702187
1101	10-11	0.33578468671360895
1101	12-13	0.34422007583619774
1101	14-15	0.4503578636458556
1101	16-17	0.4508508044844035
1101	18-19	0.4062294078067268
1101	20-21	0.36534621309188964
1101	22-23	0.3713439027551857
1101	24-25	0.41450272258144594
1101	26-27	0.4347717349528182
1101	28-29	0.43857063227927284
1101	30-31	0.4288727882896026
1101	32-33	0.4468330211272118
1101	34-35	0.5703730942515364
1101	36-37	0.5260248805175394
1101	38-39	0.462294562920178
1101	40-41	0.47887211913879923
1101	42-43	0.4332489871983469
1101	44-45	0.5136967996621493
1101	46-47	0.5289123198112691
1101	48-49	0.5017961165734448
1101	50-51	0.4702083089849296
1101	52-53	0.48824689636074936
1101	54-55	0.4887371662312461
1101	56-57	0.48795556192419554
1101	58-59	0.5500775680876515
1101	60-61	0.5688116028105341
1101	62-63	0.5353730697830983
1101	64-65	0.4930955914485011
1101	66-67	0.4849374954523711
1101	68-69	0.47666128438196864
1101	70-71	0.4752923643430833
1101	72-73	0.42528162155694815
1101	74-75	0.38827276248888865
1101	76-77	0.3734806371998758
1101	78-79	0.4022981101250451
1101	80-81	0.3945338723796077
1101	82-83	0.3988267664679448
1101	84-85	0.37054846852143797
1101	86-87	0.2878783923466486
1101	88-89	0.3202624252577486
1101	90-91	0.39013957668834465
1101	92-93	0.37645323283975074
1101	94-95	0.369048735785352
1101	96-97	0.4063484081507234
1101	98-99	0.4041088062535998
1101	100	0.42007300812365855
1102	1	0.1620081151329842
1102	2	0.21857846151994664
1102	3	0.1950266567564185
1102	4	0.14825168591701754
1102	5	0.16425552095422802
1102	6	0.13827487167307595
1102	7	0.18678642905977938
1102	8	0.22187925676811915
1102	9	0.2934080412354447
1102	10-11	0.2892621397346389
1102	12-13	0.29884373454279967
1102	14-15	0.3967472854730971
1102	16-17	0.41753204518397524
1102	18-19	0.3686066409297055
1102	20-21	0.36010775983073984
1102	22-23	0.35941818586353946
1102	24-25	0.3759516176878037
1102	26-27	0.3955435593011316
1102	28-29	0.3641712856566741
1102	30-31	0.4039235699720649
1102	32-33	0.4157598752556382
1102	34-35	0.47042666565259594
1102	36-37	0.40553195429750133
1102	38-39	0.41936461776153067
1102	40-41	0.5416541035652358
1102	42-43	0.5357306757495408
1102	44-45	0.5151204365352129
1102	46-47	0.536112847645839
1102	48-49	0.47197050314899514
1102	50-51	0.4771401819656802
1102	52-53	0.46872277283311803
1102	54-55	0.4572311737836259
1102	56-57	0.504582587441476
1102	58-59	0.4991307309902737
1102	60-61	0.4381075086481232
1102	62-63	0.4734855285673518
1102	64-65	0.502050725730264
1102	66-67	0.4261985396016783
1102	68-69	0.4953905857153771
1102	70-71	0.46937972908575176
1102	72-73	0.4274292646904243
1102	74-75	0.44045403631291435
1102	76-77	0.41593869622922597
1102	78-79	0.4554968609033523
1102	80-81	0.47256813430590583
1102	82-83	0.3902225754852431
1102	84-85	0.3829538299314059
1102	86-87	0.41889139144491594
1102	88-89	0.379011120362744
1102	90-91	0.3363353689343178
1102	92-93	0.328711573328043
1102	94-95	0.38201237106051167
1102	96-97	0.38047888532576124
1102	98-99	0.45189773141188994
1102	100	0.4551524265152018
1103	1	0.1552261990378625
1103	2	0.23104618217949024
1103	3	0.23841622261081596
1103	4	0.19989932567645496
1103	5	0.16519281874472114
1103	6	0.16799915808184807
1103	7	0.21091007848137622
1103	8	0.2213798529144313
1103	9	0.3045533120477515
1103	10-11	0.30583167098843944
1103	12-13	0.31510419249842414
1103	14-15	0.4034343704382053
1103	16-17	0.3817416138815659
1103	18-19	0.36045540074168514
1103	20-21	0.350739246139689
1103	22-23	0.2994008534863468
1103	24-25	0.3319552261626697
1103	26-27	0.38021618887439956
1103	28-29	0.3561971972371154
1103	30-31	0.37217337251747296
1103	32-33	0.36246000855653193
1103	34-35	0.35621236007656876
1103	36-37	0.35890786500381466
1103	38-39	0.3730360754653006
1103	40-41	0.46483718589480105
1103	42-43	0.41324633377920605
1103	44-45	0.4522259049512627
1103	46-47	0.4632402366886055
1103	48-49	0.46216425591206445
1103	50-51	0.4268952008781781
1103	52-53	0.40998330162806695
1103	54-55	0.4116018556925667
1103	56-57	0.4208105582167576
1103	58-59	0.4228673223763977
1103	60-61	0.4177520567122315
1103	62-63	0.4814640277371325
1103	64-65	0.4111385740278237
1103	66-67	0.41528687816081344
1103	68-69	0.3532148102079091
1103	70-71	0.35873687480319916
1103	72-73	0.36305956437390563
1103	74-75	0.3806871944512551
1103	76-77	0.315767216952878
1103	78-79	0.3771631797890507
1103	80-81	0.3721040485322682
1103	82-83	0.31791125254406793
1103	84-85	0.35022301577267356
1103	86-87	0.34715158144079084
1103	88-89	0.3635834655148038
1103	90-91	0.39069644877365306
1103	92-93	0.3744147648025624
1103	94-95	0.34210813994275924
1103	96-97	0.43296193454529686
1103	98-99	0.43297275260621504
1103	100	0.41446761289933676
1104	1	0.10769468213015188
1104	2	0.18122671324828588
1104	3	0.15591728428630347
1104	4	0.12709973684826537
1104	5	0.09380409980118287
1104	6	0.13607035701075176
1104	7	0.11017238496280868
1104	8	0.10744146236679342
1104	9	0.16500102847319909
1104	10-11	0.18961938163265302
1104	12-13	0.233200555669562
1104	14-15	0.35530920612043815
1104	16-17	0.28148769481229863
1104	18-19	0.2664667931975586
1104	20-21	0.2277648334891964
1104	22-23	0.17771070153943214
1104	24-25	0.18311811735944872
1104	26-27	0.2468262858139738
1104	28-29	0.22633010574917733
1104	30-31	0.14152741771141564
1104	32-33	0.13628366651808932
1104	34-35	0.043797830390801096
1104	36-37	0.05816229699590281
1104	38-39	0.1047767744443604
1104	40-41	0.1059075944312724
1104	42-43	0.10140230732957178
1104	44-45	0.2153287830054893
1104	46-47	0.14944044288404967
1104	48-49	0.09255750290837739
1104	50-51	0.060591878205755734
1104	52-53	0.045697885226609
1104	54-55	0.026890930853198824
1104	56-57	0.054741646823096346
1104	58-59	0.02096810390968784
1104	60-61	0.036818672625813065
1104	62-63	-0.005618371330889715
1104	64-65	-0.008771604696512725
1104	66-67	-0.014289216692112916
1104	68-69	-0.017060297567489613
1104	70-71	0.04014470012842963
1104	72-73	0.049566857419598875
1104	74-75	0.06510940190739234
1104	76-77	0.07997372174995121
1104	78-79	0.06540994570658398
1104	80-81	0.11675974358410812
1104	82-83	0.09043659658670222
1104	84-85	0.07632945641466549
1104	86-87	0.0726620433237386
1104	88-89	0.09056823862804819
1104	90-91	0.11327614450578238
1104	92-93	0.15340194397214546
1104	94-95	0.131896836630375
1104	96-97	0.1766675723280109
1104	98-99	0.22954051719758795
1104	100	0.2750499744964614
1105	1	0.09533578200799298
1105	2	0.17093113321634235
1105	3	0.14709087063105386
1105	4	0.12704230785623594
1105	5	0.06037114828767187
1105	6	0.13650954799315684
1105	7	0.14910184921141223
1105	8	0.16887248070577954
1105	9	0.1904461452777042
1105	10-11	0.19015709954381066
1105	12-13	0.15232615249797732
1105	14-15	0.3237101856015627
1105	16-17	0.21916417612890626
1105	18-19	0.24209752709204935
1105	20-21	0.2083920279826117
1105	22-23	0.2272668174215866
1105	24-25	0.21530490500516208
1105	26-27	0.20413695752624506
1105	28-29	0.19954615670992126
1105	30-31	0.23644253054543185
1105	32-33	0.17596368042174504
1105	34-35	0.21808680025524296
1105	36-37	0.23801227406327996
1105	38-39	0.2288448450990188
1105	40-41	0.2460665947926728
1105	42-43	0.2609330697198118
1105	44-45	0.1901342014359173
1105	46-47	0.15202493610575374
1105	48-49	0.1785170119991193
1105	50-51	0.1310040717787686
1105	52-53	0.12423718635751868
1105	54-55	0.12916594366220124
1105	56-57	0.14292580017782086
1105	58-59	0.16161677191789892
1105	60-61	0.12228319610485983
1105	62-63	0.15020173391050662
1105	64-65	0.09000924116565301
1105	66-67	0.1290262158433677
1105	68-69	0.08875405345988696
1105	70-71	0.10193554228109747
1105	72-73	0.13764468919253403
1105	74-75	0.13225454209334941
1105	76-77	0.19890678813401763
1105	78-79	0.14527660081704852
1105	80-81	0.15880100933216923
1105	82-83	0.15065768362248022
1105	84-85	0.15544439267213406
1105	86-87	0.14930997189595274
1105	88-89	0.15591584382022106
1105	90-91	0.14505390144752894
1105	92-93	0.18919527986061624
1105	94-95	0.17047090241669238
1105	96-97	0.1745561642573783
1105	98-99	0.2312504629209009
1105	100	0.2940346314259976
1106	1	0.1425348063833809
1106	2	0.18012158233574382
1106	3	0.14392470969189475
1106	4	0.1402524218818897
1106	5	0.09300064502049565
1106	6	0.10378599461316895
1106	7	0.12186775066787447
1106	8	0.15490291936018963
1106	9	0.18906880917032254
1106	10-11	0.19649058711256373
1106	12-13	0.21728993486036075
1106	14-15	0.27516424235363957
1106	16-17	0.240370707987843
1106	18-19	0.20850742858319649
1106	20-21	0.1776560860817895
1106	22-23	0.1772636154156757
1106	24-25	0.24435321857530568
1106	26-27	0.21536284786056115
1106	28-29	0.26033053814830254
1106	30-31	0.23839262136364425
1106	32-33	0.2146518532027173
1106	34-35	0.21544753139377093
1106	36-37	0.23481748085275456
1106	38-39	0.24086063641594535
1106	40-41	0.3133288272749155
1106	42-43	0.31021867997806396
1106	44-45	0.24848738975590123
1106	46-47	0.2791536052347965
1106	48-49	0.24729894053341184
1106	50-51	0.27790062862793263
1106	52-53	0.28419759951026435
1106	54-55	0.30914137090778127
1106	56-57	0.34956395551350283
1106	58-59	0.3260095043917204
1106	60-61	0.3406448458803055
1106	62-63	0.2627629153552533
1106	64-65	0.2963100384031989
1106	66-67	0.33116615392043514
1106	68-69	0.32236559338722515
1106	70-71	0.3723536484843848
1106	72-73	0.37012587367389926
1106	74-75	0.3247749112587712
1106	76-77	0.317955510424369
1106	78-79	0.307336896009204
1106	80-81	0.2367083997439714
1106	82-83	0.227498722278888
1106	84-85	0.25607367608360576
1106	86-87	0.30482323960237423
1106	88-89	0.3060598320887138
1106	90-91	0.35467855568370865
1106	92-93	0.3981421215353045
1106	94-95	0.41437760171978155
1106	96-97	0.3668232871752828
1106	98-99	0.427293439304524
1106	100	0.4975428350817346
1107	1	0.10041883742115232
1107	2	0.12276465763725497
1107	3	0.08478419987903862
1107	4	0.09862791020445627
1107	5	0.10181406042663355
1107	6	0.04206717418897199
1107	7	-0.008365315730451073
1107	8	0.02656934291686497
1107	9	0.05667776119499024
1107	10-11	0.06762402061994521
1107	12-13	0.013115938032328245
1107	14-15	0.11769200496014776
1107	16-17	0.1262678302096063
1107	18-19	0.10001388034016401
1107	20-21	0.02164103416441776
1107	22-23	0.09019520553831484
1107	24-25	0.09340358329809106
1107	26-27	0.09197543322950708
1107	28-29	0.07596531083668623
1107	30-31	0.07339543971735907
1107	32-33	0.08115735009534575
1107	34-35	0.07969625747264786
1107	36-37	0.11247220715042516
1107	38-39	-0.003471615697208108
1107	40-41	-0.009313871035068644
1107	42-43	0.0159739061763986
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2216	74-75	-1.0078096937695022
2216	76-77	-0.9455650355948606
2216	78-79	-0.9020030674124868
2216	80-81	-0.993671282988629
2216	82-83	-1.0142949910270325
2216	84-85	-0.974612030108549
2216	86-87	-1.0395309570908893
2216	88-89	-1.0019399478550888
2216	90-91	-0.9987382374194844
2216	92-93	-1.001425295621285
2216	94-95	-1.0542062525187283
2216	96-97	-1.228970166177799
2216	98-99	-1.3282242387377643
2216	100	-1.3680230258560755
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	470.0
3	1467.0
4	897.0
5	643.0
6	806.0
7	1669.0
8	3012.0
9	4825.0
10	6469.0
11	8035.0
12	10495.0
13	14959.0
14	17719.0
15	25096.0
16	34222.0
17	50961.0
18	56562.0
19	30179.0
20	18273.0
21	16296.0
22	16140.0
23	16932.0
24	18843.0
25	22218.0
26	27725.0
27	35017.0
28	44241.0
29	55627.0
30	70000.0
31	87768.0
32	111518.0
33	139817.0
34	188948.0
35	307209.0
36	485395.0
37	875051.0
38	1021772.0
39	493642.0
40	2814.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.63793695092257	20.847235138363985	15.508340388844063	23.006487521869385
2	14.741940527303726	21.55501312292251	38.722728420725424	24.980317929048333
3	16.88907915701353	27.667683614329597	31.48536573688925	23.957871491767627
4	11.416521997337052	18.704708625053897	37.54173153738926	32.33703784021979
5	11.894744681746712	38.399183440476314	34.27484546106794	15.431226416709032
6	28.08009377084426	38.885226928958595	17.404848404110155	15.629830896086991
7	24.654788964718442	35.25967844445493	21.481002985383924	18.604529605442703
8	26.39488293909058	34.024911812295485	19.150793804981436	20.429411443632493
9	26.53062215696995	15.596549462362608	21.60464617140933	36.268182209258114
10-11	24.58305952115773	27.157333459165496	26.693616492710643	21.565990526966132
12-13	26.09124478575453	25.582899217620337	27.821879339422516	20.50397665720262
14-15	22.961980529783066	26.209302519212567	27.80268295999845	23.026033991005917
16-17	19.803898576507518	29.343273357368126	28.77803249600114	22.074795570123218
18-19	19.67612701249754	25.878014641055458	34.06191688106478	20.38394146538222
20-21	21.30174811944866	24.642299754008807	34.32685004528495	19.72910208125758
22-23	20.973004339769442	25.032425691509097	34.32173872016119	19.67283124856027
24-25	20.38441781554578	24.952303659799334	34.45319028324537	20.21008824140951
26-27	20.18329304406471	25.274230687748457	34.57267934275297	19.96979692543386
28-29	19.95114158128971	25.250343020791714	34.92151140771801	19.877003990200564
30-31	20.412794251063787	25.300592737619787	34.370363003365846	19.916250007950584
32-33	19.911689603863707	25.227983314855827	34.68030413117019	20.18002295011028
34-35	20.42182726670229	25.041449205540996	34.4565045561457	20.080218971611007
36-37	20.041597640834382	25.338178874481404	34.2008702314353	20.41935325324891
38-39	20.293965237207377	25.141751535644048	34.040895076629845	20.52338815051873
40-41	20.373609418721735	24.992885122452797	34.03036968568373	20.60313577314174
42-43	20.396445087316838	25.405892543812225	34.009397845639924	20.188264523231005
44-45	20.729164064747767	25.196959478524573	33.319421740292874	20.754454716434783
46-47	20.754416313661856	25.42519295045798	32.823902416291	20.99648831958917
48-49	20.75959748466075	25.688268711761726	31.850787436995674	21.701346366581856
50-51	20.997428687566746	25.954620701059923	31.43587012646093	21.612080484912404
52-53	21.195965349548786	26.222949789132933	31.03012226462294	21.55096259669535
54-55	21.191259721022597	26.178558166896348	31.028161520148274	21.60202059193278
56-57	21.20818217679524	26.070340944849747	30.981210580712883	21.740266297642137
58-59	21.495783327533463	25.578359121644244	31.33557625775335	21.59028129306894
60-61	21.10823959001648	26.04403973831587	31.098257366515515	21.74946330515213
62-63	21.071564287635624	25.616722364553983	31.623513428437217	21.688199919373176
64-65	21.26105985664786	25.727951955835852	31.389449078026782	21.6215391094895
66-67	21.24797657206387	25.673556632969657	31.26796627076594	21.81050052420053
68-69	21.51864963897844	25.787201387329333	31.284861610522274	21.40928736316995
70-71	21.34472153910308	25.988288306832573	31.045723895179904	21.621266258884436
72-73	21.500523159159727	25.789595654864826	31.08298571696858	21.626895469006868
74-75	21.444021904736026	25.761539969320417	30.72967795689941	22.06476016904415
76-77	21.461563760196054	25.528073895421826	30.59433378387005	22.41602856051208
78-79	21.438456289232064	25.569710189960638	31.044034737285315	21.947798783521982
80-81	20.62087798226162	26.00215508269245	31.171948215106767	22.20501871993916
82-83	20.981023040386447	26.064446564803838	31.048232985224082	21.906297409585633
84-85	21.04250747621834	25.603058484495953	30.909346371518904	22.445087667766803
86-87	21.150596423482817	25.866129753894292	30.579223062301626	22.404050760321265
88-89	21.067135983451333	25.725461245054042	30.568545876571445	22.638856894923183
90-91	20.866071255110167	25.812966668609434	30.387151655097956	22.93381042118244
92-93	20.819398099669904	25.893740867967356	30.770464570748935	22.516396461613805
94-95	20.633679423239	25.759112729466118	30.901024855379568	22.706182991915316
96-97	20.673132019501292	26.320187496234432	30.295994403884528	22.710686080379748
98-99	20.348751621149212	26.13437657380847	30.761898208886702	22.754973596155615
100	20.68726498814215	26.143101839666695	30.46466027617897	22.704972896012183
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4734.0
1	2973.5
2	3968.0
3	7495.0
4	5203.5
5	3140.0
6	9052.0
7	24326.5
8	39609.0
9	41566.5
10	26452.0
11	10813.5
12	7015.5
13	6772.5
14	7177.5
15	7647.0
16	8064.5
17	8727.0
18	9364.5
19	9868.5
20	10609.0
21	11715.5
22	13665.0
23	16877.0
24	20916.5
25	26344.0
26	33571.0
27	41661.5
28	51041.5
29	61633.0
30	70581.5
31	77710.5
32	84023.5
33	91022.0
34	97732.5
35	103433.5
36	108281.0
37	112129.5
38	115811.5
39	117926.5
40	119898.0
41	121171.0
42	120672.0
43	123541.5
44	126899.5
45	129684.0
46	133393.5
47	134354.0
48	135817.0
49	144930.5
50	156622.0
51	151967.0
52	138920.0
53	130849.0
54	127167.0
55	124212.0
56	107730.5
57	88863.0
58	82030.5
59	81354.5
60	70828.0
61	54618.5
62	46353.5
63	37763.0
64	28765.5
65	22516.5
66	17744.0
67	13886.5
68	10993.5
69	8241.5
70	5882.5
71	4401.5
72	3434.0
73	2641.0
74	2191.0
75	1787.0
76	1170.5
77	882.0
78	650.0
79	329.0
80	141.0
81	54.0
82	30.5
83	15.5
84	7.5
85	8.0
86	7.0
87	2.0
88	2.5
89	2.5
90	1.0
91	1.0
92	2.5
93	3.0
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.047435872528639615
2	0.0
3	2.0815351182728255E-4
4	0.06843624905521434
5	0.005226965963662873
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.011864750174155106
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.782041995202292E-4
26-27	0.0
28-29	3.35358435721733E-4
30-31	0.003457661113130971
32-33	1.2720492389445043E-4
34-35	0.0015380231707238098
36-37	0.003920224472747155
38-39	0.0013298696588965274
40-41	0.0010523316431268173
42-43	0.0018849456904359476
44-45	0.0
46-47	4.278711076449697E-4
48-49	4.856915275969926E-4
50-51	0.0014917668347621915
52-53	8.441781312995347E-4
54-55	0.0011217161470692448
56-57	0.006048015926981598
58-59	0.008291448221120087
60-61	0.001063895727117222
62-63	0.0025209703099081998
64-65	0.001006075307165199
66-67	1.1564083990404586E-4
68-69	6.70716871443466E-4
70-71	4.3943519163537425E-4
72-73	0.0
74-75	2.1971759581768712E-4
76-77	0.0
78-79	4.7412744360658806E-4
80-81	0.0
82-83	9.598189712035806E-4
84-85	5.088196955778017E-4
86-87	1.1564083990404585E-5
88-89	0.0
90-91	0.0
92-93	2.1971759581768712E-4
94-95	0.0
96-97	4.278711076449697E-4
98-99	5.666401155298247E-4
100	3.2379435173132844E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4323732.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.68670564190769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.84504715645646	23.684227932371822
2	17.21068758034485	12.972282334705856
3	7.449077500365449	8.421935731800906
4	3.9565178857504164	5.964324997088756
5	2.351921340686819	4.431808362969252
6	1.5188128559945588	3.434343181740724
7	1.0261095605629675	2.706948227569866
8	0.7232257484395173	2.1804796715270776
9	0.5162621363847504	1.7510597251195037
>10	2.2318101674034354	13.934537426398045
>50	0.0912507593832674	2.361133456336425
>100	0.059716233473862096	4.550154352362449
>500	0.010395665626719989	2.7544107083115983
>1k	0.008611793810556153	5.905607046705043
>5k	2.460512517301755E-4	0.701770127804163
>10k+	3.075640646627194E-4	4.244976717188542
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63149	1.4605206798201182	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53715	1.2423295430891645	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40134	0.9282258937417952	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13624	0.3150981605705442	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12492	0.2889170744162681	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9378	0.2168959593240284	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8434	0.19506296875014456	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6843	0.15826605349267717	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	5617	0.12991091954820513	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4966	0.11485448219269835	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	4863	0.112472280890675	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	4560	0.10546444599248983	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	4543	0.10507126713681605	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	2.312816798080917E-5	0.0
14-15	0.0	0.0	0.0	2.312816798080917E-5	0.0
16-17	0.0	0.0	0.0	8.09485879328321E-5	0.0
18-19	0.0	0.0	0.0	1.8502534384647336E-4	0.0
20-21	0.0	0.0	0.0	3.5848660370254215E-4	0.0
22-23	0.0	0.0	0.0	9.019985512515576E-4	0.0
24-25	0.0	0.0	0.0	0.0018386893544743291	0.0
26-27	0.0	0.0	0.0	0.003330456189236521	0.0
28-29	0.0	0.0	0.0	0.006603091958521019	0.0
30-31	0.0	0.0	0.0	0.015426488043199717	0.0
32-33	0.0	0.0	0.0	0.030425104978754464	0.0
34-35	0.0	0.0	0.0	0.052581889904369655	0.0
36-37	0.0	0.0	0.0	0.08781765382313242	0.0
38-39	0.0	0.0	0.0	0.13602831997912915	0.0
40-41	0.0	0.0	0.0	0.18692185362089975	0.0
42-43	0.0	0.0	0.0	0.23727187531512128	0.0
44-45	0.0	0.0	0.0	0.299116596495805	0.0
46-47	0.0	0.0	0.0	0.37546267900045605	0.0
48-49	0.0	0.0	0.0	0.45602965216160485	0.0
50-51	0.0	0.0	0.0	0.5403433885356447	0.0
52-53	0.0	0.0	0.0	0.6223674362795844	0.0
54-55	0.0	0.0	0.0	0.7131801878562316	0.0
56-57	0.0	0.0	0.0	0.8240681892402212	0.0
58-59	0.0	0.0	0.0	0.9491337575964468	0.0
60-61	0.0	0.0	0.0	1.083681874824804	0.0
62-63	0.0	0.0	0.0	1.2207393982790793	0.0
64-65	0.0	0.0	0.0	1.3610811215866292	0.0
66-67	0.0	0.0	0.0	1.5257999339459523	0.0
68-69	0.0	0.0	0.0	1.7166767042915705	0.0
70-71	0.0	0.0	0.0	1.9238357048956782	0.0
72-73	0.0	0.0	0.0	2.138291642497731	0.0
74-75	0.0	0.0	0.0	2.3578126488875815	0.0
76-77	0.0	0.0	0.0	2.5930376813364013	0.0
78-79	0.0	0.0	0.0	2.863590990375907	0.0
80-81	0.0	0.0	0.0	3.162036870000268	0.0
82-83	0.0	0.0	0.0	3.47364267720571	0.0
84-85	0.0	0.0	0.0	3.784022691508169	0.0
86-87	0.0	0.0	0.0	4.119485203985816	0.0
88	0.0	0.0	0.0	4.386580851912191	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18605	0.0	58.12238	1
GTATCAA	34290	0.0	42.55566	1
GTGGTAT	6900	0.0	33.349	1
TATCAAC	46385	0.0	31.38086	2
ATCAACG	46375	0.0	31.34706	3
TGGTATC	7350	0.0	31.033884	2
TCAACGC	47095	0.0	30.887434	4
CAACGCA	50695	0.0	28.642666	5
AACGCAG	51690	0.0	28.1449	6
ACGCAGA	55585	0.0	25.961296	7
CGCAGAG	55790	0.0	25.815348	8
GCAGAGT	58400	0.0	24.27527	9
GAGTACT	42455	0.0	22.70899	12-13
GTACATG	26625	0.0	22.28684	1
AGTACTT	42965	0.0	22.144104	12-13
TCGATTA	1135	0.0	21.973692	1
CATGGGT	6190	0.0	21.95755	4
GCGTATC	130	3.1358624E-4	21.718557	1
CGATTAT	1160	0.0	21.488178	2
TACATGG	27015	0.0	21.482853	2
>>END_MODULE