FastQCFastQC Report
Wed 25 May 2016
SRR2431435_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431435_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5910354
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA335600.567817088451893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76300.048.8577041
GTATCAA135200.036.369671
ATCAACG157700.030.4615573
TCAACGC157250.030.3992754
TATCAAC171100.028.4330372
GTACATG300050.024.8794751
GTGGTAT36100.024.741771
CAACGCA198700.024.1287735
TACATGG297950.023.9317872
ACGCAGA197850.023.4943687
CGCAGAG197350.023.244098
AACGCAG206200.023.1811966
TAGACCG1903.6422352E-722.2649565
ACATGGG315700.021.9013543
TGGTATC40100.021.5678252
GCAGAGT215650.020.4434059
TACCTGG38450.019.4371992
CATGGGA145450.019.4219884
GTTAGAC7800.018.0783843
CATGGGG145300.017.9539624
Produced by FastQC (version 0.11.5)