##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431435_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5910354
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.933066445766194	31.0	30.0	34.0	26.0	34.0
2	29.995005882896354	31.0	31.0	34.0	25.0	34.0
3	30.070926377675516	31.0	31.0	34.0	25.0	34.0
4	33.39186248404072	37.0	35.0	37.0	27.0	37.0
5	33.48593366827097	37.0	35.0	37.0	28.0	37.0
6	33.56047556542298	37.0	35.0	37.0	28.0	37.0
7	33.43584631309732	37.0	35.0	37.0	27.0	37.0
8	33.38691269592312	37.0	35.0	37.0	27.0	37.0
9	34.680010199050685	39.0	35.0	39.0	25.0	39.0
10-11	34.635497129275166	39.0	35.0	39.0	26.0	39.0
12-13	34.5850894887176	39.0	35.0	39.0	25.5	39.0
14-15	35.73288858839928	40.0	36.0	41.0	25.5	41.0
16-17	35.74224496536079	40.0	36.0	41.0	25.5	41.0
18-19	35.726834890092874	40.0	36.0	41.0	25.5	41.0
20-21	35.8108266442247	40.0	36.0	41.0	26.0	41.0
22-23	35.76418696748114	40.0	36.0	41.0	26.0	41.0
24-25	35.779772412955296	40.0	36.0	41.0	27.0	41.0
26-27	35.75276861589002	40.0	36.0	41.0	27.0	41.0
28-29	35.64106828457314	40.0	36.0	41.0	26.0	41.0
30-31	35.61444483697592	40.0	35.0	41.0	26.0	41.0
32-33	35.56045779998965	40.0	35.0	41.0	26.0	41.0
34-35	35.4589253875487	40.0	35.0	41.0	25.0	41.0
36-37	35.33450407539041	40.0	35.0	41.0	25.0	41.0
38-39	35.23966643960751	40.0	35.0	41.0	25.0	41.0
40-41	35.150714576487296	40.0	35.0	41.0	25.0	41.0
42-43	34.92023007420537	39.0	35.0	41.0	24.0	41.0
44-45	34.78175385095376	39.0	34.0	41.0	24.0	41.0
46-47	34.639834855915566	39.0	34.0	41.0	23.0	41.0
48-49	34.49817752033127	39.0	34.0	41.0	23.0	41.0
50-51	34.31327184463063	39.0	34.0	40.5	23.0	41.0
52-53	34.191022229802144	38.0	33.0	40.0	22.0	41.0
54-55	34.090928225280585	38.0	33.0	40.0	22.0	41.0
56-57	33.907110809267934	38.0	33.0	40.0	21.5	41.0
58-59	33.67319622479465	38.0	33.0	40.0	20.5	41.0
60-61	33.44016458235835	37.0	33.0	40.0	19.5	41.0
62-63	33.16652005954296	37.0	32.5	40.0	18.0	41.0
64-65	33.015000962717295	36.5	32.5	40.0	18.0	41.0
66-67	32.75589846225793	36.0	32.0	39.5	15.5	41.0
68-69	32.493380768732294	35.5	32.0	39.0	13.0	41.0
70-71	32.22123260298791	35.0	32.0	39.0	12.0	41.0
72-73	31.92221582666622	35.0	32.0	38.5	9.0	40.5
74-75	31.593980326728314	35.0	31.0	37.5	7.0	40.0
76-77	31.294289563704645	35.0	31.0	37.0	4.5	39.0
78-79	30.97641359553083	35.0	31.0	36.5	2.0	39.0
80-81	30.702822622807368	35.0	31.0	36.0	2.0	39.0
82-83	30.424832505802527	35.0	31.0	36.0	2.0	37.5
84-85	30.148779582407414	35.0	31.0	35.0	2.0	37.0
86-87	29.907019613376796	35.0	30.0	35.0	2.0	37.0
88-89	29.65771364963926	35.0	30.0	35.0	2.0	36.0
90-91	29.443853193903443	34.0	30.0	35.0	2.0	36.0
92-93	29.26904488292918	34.0	30.0	35.0	2.0	36.0
94-95	29.047002006986382	34.0	30.0	35.0	2.0	35.5
96-97	28.96664751383758	34.0	30.0	35.0	2.0	35.0
98-99	28.816711063330555	34.0	30.0	35.0	2.0	35.0
100	28.678502844330474	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3468224270332847
1101	2	0.4463837630410161
1101	3	0.37678265295959434
1101	4	0.41513374863061614
1101	5	0.23436819059790537
1101	6	0.2710422005099318
1101	7	0.31484261360206744
1101	8	0.37867548861557765
1101	9	0.4410469178206853
1101	10-11	0.532538137932832
1101	12-13	0.516927704839631
1101	14-15	0.6210678025227523
1101	16-17	0.535647932196774
1101	18-19	0.48723329046570996
1101	20-21	0.48871429390653987
1101	22-23	0.4447741456786076
1101	24-25	0.47145412493716776
1101	26-27	0.5686220635376316
1101	28-29	0.6126503495339293
1101	30-31	0.5978648267583893
1101	32-33	0.5733441766765637
1101	34-35	0.535760916665339
1101	36-37	0.5727676943067266
1101	38-39	0.5398498553307292
1101	40-41	0.4916488698067951
1101	42-43	0.5022457413881938
1101	44-45	0.4728021752884075
1101	46-47	0.3958186163728712
1101	48-49	0.3848975607843528
1101	50-51	0.3477492724899207
1101	52-53	0.31713654112292033
1101	54-55	0.37920175027839065
1101	56-57	0.45315448186956075
1101	58-59	0.3863858411293819
1101	60-61	0.43170399430429285
1101	62-63	0.46018247683672797
1101	64-65	0.4506271917317832
1101	66-67	0.3950861437103157
1101	68-69	0.2778961277032366
1101	70-71	0.3706898868912418
1101	72-73	0.32736435775914785
1101	74-75	0.3325901030534588
1101	76-77	0.2910554354896462
1101	78-79	0.29233012023014737
1101	80-81	0.30139809693364583
1101	82-83	0.2870947789032954
1101	84-85	0.2894382958166659
1101	86-87	0.2995580372880795
1101	88-89	0.2971339405757796
1101	90-91	0.2569773574772185
1101	92-93	0.2684147093305498
1101	94-95	0.27055984307749625
1101	96-97	0.3129706294602137
1101	98-99	0.25797317585622537
1101	100	0.21357934688699487
1102	1	0.2453054097925147
1102	2	0.396243205500987
1102	3	0.3885090192529539
1102	4	0.36587405444939236
1102	5	0.22175692075816755
1102	6	0.26102664871545755
1102	7	0.22455985024259206
1102	8	0.38675865968268397
1102	9	0.4742766261981899
1102	10-11	0.4918509605805994
1102	12-13	0.4852180950778404
1102	14-15	0.5456273562049816
1102	16-17	0.5148878089652058
1102	18-19	0.4949397140419052
1102	20-21	0.5193718446804212
1102	22-23	0.47177846662993517
1102	24-25	0.5028350961806964
1102	26-27	0.533946355926588
1102	28-29	0.5951938188781298
1102	30-31	0.5649135180450173
1102	32-33	0.5815261127637186
1102	34-35	0.5187065896098062
1102	36-37	0.5332910142401062
1102	38-39	0.47046892917335725
1102	40-41	0.4569485038770509
1102	42-43	0.5272275678633065
1102	44-45	0.4476011613184312
1102	46-47	0.46861381575173056
1102	48-49	0.481712934166481
1102	50-51	0.4568085001758604
1102	52-53	0.442809986228923
1102	54-55	0.4506534921408516
1102	56-57	0.44608716912419766
1102	58-59	0.45389584571704233
1102	60-61	0.45051785232332264
1102	62-63	0.4870371425068143
1102	64-65	0.4469963732395499
1102	66-67	0.5003692525351084
1102	68-69	0.41732043171504785
1102	70-71	0.4872198971410171
1102	72-73	0.4726595300570473
1102	74-75	0.49337052383606306
1102	76-77	0.43877720885093296
1102	78-79	0.41569290008999005
1102	80-81	0.4424417330087742
1102	82-83	0.4174929818395725
1102	84-85	0.4595300095383408
1102	86-87	0.46336661525037925
1102	88-89	0.448368927838942
1102	90-91	0.4004429542553751
1102	92-93	0.4093257950489786
1102	94-95	0.46531292028049975
1102	96-97	0.46199606595417464
1102	98-99	0.41084501730052736
1102	100	0.47225452254000544
1103	1	0.2851727857063473
1103	2	0.38475111549805874
1103	3	0.38318078367809605
1103	4	0.37949280418384745
1103	5	0.4068441837658838
1103	6	0.4427970000669461
1103	7	0.47454298684578333
1103	8	0.4082829015183833
1103	9	0.5334744153033171
1103	10-11	0.5893672622793247
1103	12-13	0.5265951631213426
1103	14-15	0.64481291000655
1103	16-17	0.5413243771504384
1103	18-19	0.5175715953059381
1103	20-21	0.5507343751165621
1103	22-23	0.5391676676365762
1103	24-25	0.5554558900904851
1103	26-27	0.5493611944205128
1103	28-29	0.6130177496430491
1103	30-31	0.5221031288830886
1103	32-33	0.5655241120723886
1103	34-35	0.512708913833535
1103	36-37	0.5618731516505662
1103	38-39	0.5668305561242732
1103	40-41	0.5676215786582262
1103	42-43	0.5729250602310145
1103	44-45	0.4801208147017917
1103	46-47	0.4982999274082758
1103	48-49	0.4336226115500281
1103	50-51	0.32330003755470216
1103	52-53	0.39695155321746256
1103	54-55	0.4456281358406571
1103	56-57	0.3780330400045955
1103	58-59	0.42647068179977055
1103	60-61	0.4403236424999619
1103	62-63	0.40418595381704137
1103	64-65	0.4359231174908942
1103	66-67	0.4392951036206796
1103	68-69	0.3689825420501265
1103	70-71	0.4297871530574966
1103	72-73	0.3583700377360337
1103	74-75	0.34588432289141835
1103	76-77	0.31406422286822533
1103	78-79	0.30697961605324053
1103	80-81	0.2844665704188003
1103	82-83	0.2769853519620611
1103	84-85	0.2678538234800243
1103	86-87	0.20592426340754244
1103	88-89	0.2949896333573463
1103	90-91	0.3322933738262215
1103	92-93	0.28208503605877056
1103	94-95	0.3138995868598684
1103	96-97	0.2747466443234927
1103	98-99	0.19416769788245247
1103	100	0.19679626266747974
1104	1	0.15710339935661466
1104	2	0.23446508626449614
1104	3	0.23295448770116067
1104	4	0.23367123523669875
1104	5	0.14946780316269326
1104	6	0.2675054227930431
1104	7	0.2825884959101046
1104	8	0.26622589472729885
1104	9	0.3542495701568882
1104	10-11	0.3427036601524165
1104	12-13	0.3006732163440802
1104	14-15	0.27200939823233483
1104	16-17	0.3644563030876071
1104	18-19	0.26283288121852877
1104	20-21	0.19125015756533514
1104	22-23	0.2422447574499813
1104	24-25	0.2246216327281303
1104	26-27	0.3771056779727928
1104	28-29	0.382185359117571
1104	30-31	0.32592743258055634
1104	32-33	0.2972631888122024
1104	34-35	0.3093276483589591
1104	36-37	0.32711287835890346
1104	38-39	0.2885575809960699
1104	40-41	0.29742217590241893
1104	42-43	0.28499497387930717
1104	44-45	0.30503570792866697
1104	46-47	0.2756827720721944
1104	48-49	0.256712927184644
1104	50-51	0.18517703736227986
1104	52-53	0.1814053048746871
1104	54-55	0.18510192368359668
1104	56-57	0.2754893822569926
1104	58-59	0.24543455613138576
1104	60-61	0.2639728009983884
1104	62-63	0.2956602851967105
1104	64-65	0.2600721519385658
1104	66-67	0.24845002744158506
1104	68-69	0.3073578013361029
1104	70-71	0.24382010642877106
1104	72-73	0.23769583843782627
1104	74-75	0.28417799781612985
1104	76-77	0.24459499082526293
1104	78-79	0.300709667686192
1104	80-81	0.3053013328765104
1104	82-83	0.2245090646565302
1104	84-85	0.22984874607036332
1104	86-87	0.2635323223324235
1104	88-89	0.31765560123051984
1104	90-91	0.2873135549955599
1104	92-93	0.2329158047006814
1104	94-95	0.223267390838906
1104	96-97	0.344477516591418
1104	98-99	0.3104722147660617
1104	100	0.3045363064066926
1105	1	0.08208285568503015
1105	2	0.16924950234178837
1105	3	0.1736618332497386
1105	4	0.20752002577326323
1105	5	0.15126020835626264
1105	6	0.25799354203800107
1105	7	0.24037796468532946
1105	8	0.22471079291752005
1105	9	0.32561772240036646
1105	10-11	0.39146599439594354
1105	12-13	0.2706828328729358
1105	14-15	0.35402439873085
1105	16-17	0.4172051347919208
1105	18-19	0.38529780822617
1105	20-21	0.3871901290373643
1105	22-23	0.3668436332166536
1105	24-25	0.3373140035080411
1105	26-27	0.3612557127080862
1105	28-29	0.47318506000814153
1105	30-31	0.4318116064756907
1105	32-33	0.4212984001024509
1105	34-35	0.4239442294738893
1105	36-37	0.3869290949141302
1105	38-39	0.4153742486276002
1105	40-41	0.3773703262483252
1105	42-43	0.434125566501919
1105	44-45	0.3619887155865058
1105	46-47	0.3464730280198154
1105	48-49	0.28378098342365377
1105	50-51	0.3006894994509608
1105	52-53	0.30028097907698736
1105	54-55	0.3187709912662413
1105	56-57	0.3561596969329699
1105	58-59	0.3297035511880395
1105	60-61	0.22555496925226493
1105	62-63	0.25600977285121473
1105	64-65	0.25748674494068524
1105	66-67	0.2981253489842146
1105	68-69	0.34572594232277964
1105	70-71	0.32997739054734865
1105	72-73	0.31621451508767606
1105	74-75	0.3420269065491617
1105	76-77	0.3230200148665858
1105	78-79	0.3469702370232959
1105	80-81	0.377420816639372
1105	82-83	0.30038524621916807
1105	84-85	0.3058258971656542
1105	86-87	0.2801704047515443
1105	88-89	0.3148874721438979
1105	90-91	0.3067677687397996
1105	92-93	0.31715376313412236
1105	94-95	0.36832123053554966
1105	96-97	0.3617549052053235
1105	98-99	0.4431964744702306
1105	100	0.44638308197492194
1106	1	0.2427926000374221
1106	2	0.26429195557983576
1106	3	0.21262892393673027
1106	4	0.271883417764748
1106	5	0.2457655484404242
1106	6	0.3067704114693228
1106	7	0.3637608143731157
1106	8	0.3765396001394947
1106	9	0.4188981364829374
1106	10-11	0.4982232844045029
1106	12-13	0.40689834808375736
1106	14-15	0.4091861711251781
1106	16-17	0.37039297453871
1106	18-19	0.43481449972426134
1106	20-21	0.44989360117128285
1106	22-23	0.4942676458938351
1106	24-25	0.4488677698531234
1106	26-27	0.4848153444796992
1106	28-29	0.5714117232369134
1106	30-31	0.5714737889676442
1106	32-33	0.5151501377898384
1106	34-35	0.4793726009695689
1106	36-37	0.5076200462044866
1106	38-39	0.4842261191899837
1106	40-41	0.5219380009112129
1106	42-43	0.45662306683151144
1106	44-45	0.37382735944741796
1106	46-47	0.3779626980581341
1106	48-49	0.386140402647122
1106	50-51	0.3868534526805121
1106	52-53	0.4412975078194066
1106	54-55	0.4991423982776624
1106	56-57	0.49689377879978736
1106	58-59	0.4471577517686143
1106	60-61	0.44180004623355984
1106	62-63	0.45090931740757156
1106	64-65	0.5144869823008094
1106	66-67	0.5155980937292668
1106	68-69	0.45192458046422956
1106	70-71	0.49554952371739347
1106	72-73	0.44808396779953696
1106	74-75	0.43607957716163526
1106	76-77	0.4249654474473026
1106	78-79	0.44548801965198237
1106	80-81	0.37647250397685283
1106	82-83	0.3630973714322714
1106	84-85	0.39862533616092577
1106	86-87	0.36894971389747866
1106	88-89	0.42808257529184246
1106	90-91	0.4429132037317096
1106	92-93	0.4239672171361022
1106	94-95	0.5082303984826773
1106	96-97	0.5193257596641594
1106	98-99	0.5107085955868946
1106	100	0.4524413133736864
1107	1	0.08108267581313555
1107	2	0.04148448068709598
1107	3	0.10948983809418422
1107	4	0.11538937312623432
1107	5	0.08990511839439819
1107	6	0.14183112136544196
1107	7	0.20399673378952343
1107	8	0.16210366403959853
1107	9	0.2684391541460869
1107	10-11	0.24183354159023907
1107	12-13	0.18489098477465404
1107	14-15	0.2850371734746062
1107	16-17	0.21752627426980808
1107	18-19	0.2749016542980627
1107	20-21	0.25789681057754166
1107	22-23	0.32607222275034076
1107	24-25	0.24808460579860991
1107	26-27	0.2597128265070978
1107	28-29	0.31627046383590596
1107	30-31	0.26755330352573736
1107	32-33	0.254091753020127
1107	34-35	0.2757378709160676
1107	36-37	0.24194835579613994
1107	38-39	0.2724711162651161
1107	40-41	0.31383878637406326
1107	42-43	0.31563920864284967
1107	44-45	0.2914020510237805
1107	46-47	0.20080048278644824
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2216	2	-0.9601472080566822
2216	3	-0.9198068294069692
2216	4	-0.772345659651954
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2216	12-13	-1.4098728532151554
2216	14-15	-1.6310451819214578
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2216	56-57	-1.6354168455302869
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2216	60-61	-1.6965405002017562
2216	62-63	-1.8488332169386439
2216	64-65	-1.736940236520514
2216	66-67	-1.8381941034056837
2216	68-69	-1.8321867606950448
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2216	80-81	-1.5802746779677044
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2216	84-85	-1.6143410108159486
2216	86-87	-1.7000128322828303
2216	88-89	-1.6802280400703147
2216	90-91	-1.822769865720062
2216	92-93	-1.729340552911708
2216	94-95	-1.6634863533821154
2216	96-97	-1.7938732491417966
2216	98-99	-2.0174188173142547
2216	100	-1.974712712561871
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	196532.0
3	40837.0
4	27489.0
5	22999.0
6	17000.0
7	15365.0
8	16321.0
9	17462.0
10	17761.0
11	18249.0
12	19225.0
13	20667.0
14	21149.0
15	21268.0
16	21145.0
17	20706.0
18	20338.0
19	20124.0
20	20356.0
21	22807.0
22	28086.0
23	40417.0
24	57072.0
25	69674.0
26	76545.0
27	70881.0
28	76027.0
29	89747.0
30	105646.0
31	123380.0
32	154183.0
33	200884.0
34	275966.0
35	410805.0
36	686457.0
37	1106043.0
38	1254825.0
39	484636.0
40	1280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.6008950868706	21.549856047640496	14.408434826487234	22.440814039001662
2	15.601259300846404	21.87920160353411	38.75874669582429	23.760792399795193
3	18.401456291951476	27.98141867310659	29.241985013377768	24.375140021564167
4	12.572171345205177	19.116569603448045	37.78358799667135	30.52767105467543
5	12.750359487509058	40.48277759559457	33.71600266810969	13.050860248786677
6	28.860234238847276	39.30420777415223	18.13906808386139	13.696489903139108
7	26.189854937093383	34.80883670811708	21.84267185947471	17.15863649531483
8	24.717896324191813	37.60053496819904	19.629877676110056	18.051691031499097
9	25.61704909844308	19.004091917637783	20.880353668385634	34.4985053155335
10-11	23.64162101074338	29.309562980639313	27.36960290979628	19.679213098821023
12-13	23.86209139411328	27.287572824306427	27.26672659956281	21.58360918201749
14-15	21.78698724366149	28.89614358856868	24.75207853011704	24.564790637652795
16-17	21.06207892635601	31.86989734927389	25.950048400679453	21.117975323690647
18-19	21.16283449410466	30.84311913674552	27.390517473160354	20.603528895989463
20-21	22.777643777150463	29.521233102067335	26.823807524375397	20.877315596406806
22-23	22.618561859172388	29.778749842744933	26.60328466174488	20.999403636337792
24-25	21.819583445854253	29.845277809926614	26.9914172763101	21.34372146790903
26-27	21.620382535916782	29.997194576429266	27.074545586525574	21.307877301128382
28-29	21.497295227290763	29.902803497293874	27.5093330607718	21.09056821464356
30-31	21.857552126141147	29.977955214830693	26.95125367289708	21.213238986131085
32-33	21.61660677686255	29.977270808344	27.04612217113949	21.360000243653957
34-35	22.14394215951733	29.4404401995077	27.121338703284533	21.294278937690432
36-37	21.765833792869945	29.64333472815878	27.093909091109065	21.49692238786221
38-39	21.6066431139292	29.75505327876931	27.172938001094586	21.465365606206905
40-41	21.896499562436844	29.24717140631377	27.240209989705587	21.616119041543797
42-43	21.688891483335198	29.723911432371246	27.341118364280565	21.246078720012996
44-45	21.783786646506755	29.514436270648837	27.124341315911344	21.577435766933064
46-47	21.736133289960318	29.24651510645777	27.298764926571145	21.718586677010766
48-49	21.504842997148202	29.40923564898683	27.197855073106243	21.88806628075873
50-51	21.551047404451644	29.403972972074378	27.18381106136866	21.86116856210532
52-53	21.718653159298476	29.47123062889869	27.136749497517577	21.673366714285255
54-55	21.59651767562022	29.440918309757635	27.19799434034303	21.76456967427912
56-57	21.60931919048014	29.31162724591678	27.19965753946164	21.879396024141435
58-59	21.99402980528976	28.952178799590705	27.312266055631863	21.741525339487673
60-61	21.70672330161154	29.401246875704302	27.204426229397242	21.68760359328692
62-63	21.44044565990379	29.377925154949352	27.339891309147962	21.841737875998895
64-65	21.923323177799006	29.202736410585445	27.04399163598179	21.829948775633756
66-67	21.511390780525545	29.15985670420518	27.24863698525546	22.08011553001382
68-69	21.591171833235098	29.34561405898366	27.45007997711048	21.613134130670765
70-71	21.715196608892438	29.143421992267232	27.250636226066277	21.89074517277405
72-73	21.747068107616425	29.13421698440114	27.32436394449167	21.794350963490768
74-75	21.69541743197618	29.0753064558579	27.150165464209657	22.07911064795627
76-77	21.676016484420586	28.73175829561385	26.963624582743705	22.628600637221854
78-79	21.71522898450087	28.74751824745886	27.357085401389952	22.180167366650323
80-81	21.326437689463543	29.177470191746075	27.288096934946026	22.207995183844357
82-83	21.4589182283183	29.16661379241382	27.482522309973746	21.891945669294135
84-85	21.489978667359708	28.69780711015818	27.412552756323315	22.3996614661588
86-87	21.898100697988518	28.891445675038778	27.133907115625156	22.076546511347548
88-89	21.629432608363693	28.946114354771098	27.23837707304136	22.18607596382385
90-91	21.52848101105191	28.83817318564969	27.10983941155301	22.52350639174539
92-93	21.504702459559354	28.821225321287503	27.291616788250156	22.38245543090299
94-95	21.55710588128799	28.611453620017162	27.53812776242539	22.293312736269456
96-97	21.83061517183082	28.98793804112854	26.9849434260721	22.196503360968535
98-99	21.48772894547608	28.967877839346894	27.20759697136331	22.336796243813716
100	21.46108554035824	28.62445329508521	27.325119146097215	22.589342018459334
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27486.0
1	21187.0
2	13330.0
3	10148.5
4	7207.0
5	5294.5
6	4383.0
7	4074.0
8	4191.0
9	4186.5
10	3715.5
11	3250.0
12	3134.0
13	3164.5
14	3261.5
15	3434.0
16	3674.5
17	4200.0
18	4986.5
19	5944.5
20	7484.5
21	9830.5
22	13329.5
23	18779.5
24	25945.5
25	34239.0
26	44652.0
27	57445.5
28	72889.5
29	88345.5
30	99435.5
31	109264.0
32	119056.0
33	128850.0
34	137901.0
35	146256.5
36	152127.5
37	157850.5
38	165794.5
39	170096.5
40	176728.5
41	184591.0
42	189929.5
43	201016.0
44	207591.5
45	208849.5
46	214551.0
47	216316.0
48	217662.0
49	218045.0
50	217391.5
51	208405.0
52	192583.0
53	177727.0
54	168543.0
55	165507.5
56	144621.5
57	118361.5
58	102730.5
59	93257.5
60	80183.0
61	63338.0
62	53149.0
63	44512.0
64	36008.0
65	28758.0
66	22649.5
67	17667.0
68	13560.5
69	10637.5
70	7923.5
71	5549.0
72	4130.0
73	3177.5
74	2319.5
75	1583.5
76	1148.5
77	896.5
78	647.0
79	454.5
80	320.5
81	213.0
82	167.5
83	133.5
84	97.5
85	90.5
86	85.5
87	63.0
88	51.0
89	38.0
90	28.5
91	30.0
92	29.5
93	30.5
94	23.5
95	16.0
96	21.0
97	19.0
98	18.0
99	25.0
100	42.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012858789845752047
2	0.006903139811930047
3	0.009305703177846878
4	0.00778295174874466
5	0.009339542098493593
6	0.01260499794090168
7	0.01086229352759581
8	0.00795214635197824
9	0.00695389819290012
10-11	0.00654783114513953
12-13	0.00695389819290012
14-15	0.007926767161493204
16-17	0.005295791081211041
18-19	0.005871052732205211
20-21	0.005896431922690248
22-23	0.006251740589480766
24-25	0.005786455430588421
26-27	0.006310958700612518
28-29	0.016665668418507588
30-31	0.005744156779780026
32-33	0.0056341802876782
34-35	0.005185814589109214
36-37	0.007021576034193552
38-39	0.005997948684630396
40-41	0.005921811113175286
42-43	0.00497432133506724
44-45	0.004652851588923439
46-47	0.006454774113361061
48-49	0.0037645799219471455
50-51	0.0041368080490610205
52-53	0.003333133683701518
54-55	0.004712069700055192
56-57	0.0031131806994978643
58-59	0.00273249284222231
60-61	0.0037561201917854664
62-63	0.003333133683701518
64-65	0.004669771049246796
66-67	0.0034092712551566286
68-69	0.0038153383029172192
70-71	0.00521965350975593
72-73	0.0030116639375577167
74-75	0.002766331762869026
76-77	0.002834009604162458
78-79	0.003654603429845319
80-81	0.004162187239546058
82-83	0.0017765433339525856
84-85	0.0027409525723839892
86-87	0.0026478955406055204
88-89	0.0018019225244376225
90-91	0.0025886774294737675
92-93	0.002520999588180336
94-95	0.002140311730904782
96-97	0.0013281776353835997
98-99	0.0017596238736292276
100	0.0029270666359409265
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5910354.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.34667525827149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.4415794886959	28.982733407709798
2	15.010900792043994	12.713234823496345
3	6.361683389789978	8.08188421810128
4	3.22734389187753	5.466691349444153
5	1.897562991447779	4.017774189047665
6	1.2077324613334102	3.0686072603375307
7	0.8039279456665731	2.383057295233419
8	0.5482410833554962	1.8572949696078516
9	0.41281796455851655	1.5733321457346114
>10	1.8484708774618073	13.65552885950252
>50	0.13168257158617677	3.918970559475083
>100	0.09496534810351892	7.742857351792637
>500	0.008352679827663375	2.483589689136227
>1k	0.004618043550287939	3.2882445400498255
>5k	8.031380086890953E-5	0.19550777520214638
>10k+	4.015690043445477E-5	0.570691566128906
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33560	0.567817088451893	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.691946032335796E-5	0.0
3	0.0	0.0	0.0	5.075838097007387E-5	0.0
4	0.0	0.0	0.0	5.075838097007387E-5	0.0
5	0.0	0.0	0.0	5.075838097007387E-5	0.0
6	0.0	0.0	0.0	5.075838097007387E-5	0.0
7	0.0	0.0	0.0	5.075838097007387E-5	0.0
8	0.0	0.0	0.0	5.075838097007387E-5	0.0
9	0.0	0.0	0.0	1.0151676194014774E-4	0.0
10-11	0.0	0.0	0.0	1.3535568258686367E-4	0.0
12-13	0.0	1.691946032335796E-5	0.0	1.6919460323357958E-4	0.0
14-15	0.0	1.691946032335796E-5	0.0	1.6919460323357958E-4	0.0
16-17	0.0	1.691946032335796E-5	0.0	1.9457379371861652E-4	0.0
18-19	0.0	1.691946032335796E-5	0.0	2.537919048503694E-4	0.0
20-21	0.0	1.691946032335796E-5	0.0	3.2992947630548016E-4	0.0
22-23	0.0	1.691946032335796E-5	0.0	6.260200319642444E-4	0.0
24-25	0.0	1.691946032335796E-5	0.0	0.001209741413120094	0.0
26-27	0.0	1.691946032335796E-5	0.0	0.00198803658799456	0.0
28-29	0.0	1.691946032335796E-5	0.0	0.004695150239731834	0.0
30-31	0.0	1.691946032335796E-5	0.0	0.011572910861176844	0.0
32-33	0.0	1.691946032335796E-5	0.0	0.02452475773870736	0.0
34-35	0.0	1.691946032335796E-5	0.0	0.04508190203158728	0.0
36-37	0.0	1.691946032335796E-5	0.0	0.07778721883663821	0.0
38-39	0.0	1.691946032335796E-5	0.0	0.11979823881953602	0.0
40-41	0.0	3.383892064671592E-5	0.0	0.16263831235827836	0.0
42-43	0.0	3.383892064671592E-5	0.0	0.20552068454782912	0.0
44-45	0.0	3.383892064671592E-5	0.0	0.26088961845601805	0.0
46-47	0.0	5.075838097007388E-5	0.0	0.32888046976543195	0.0
48-49	0.0	1.0151676194014774E-4	0.0	0.4011096458858471	0.0
50-51	0.0	1.0151676194014774E-4	0.0	0.47348263741901075	0.0
52-53	0.0	1.0997649210182673E-4	0.0	0.5445274513167908	0.0
54-55	0.0	1.1843622226350571E-4	0.0	0.6250894616464597	0.0
56-57	0.0	1.1843622226350571E-4	0.0	0.7214204089974983	0.0
58-59	0.0	1.522751429102216E-4	0.0	0.832319011686948	0.0
60-61	0.0	1.522751429102216E-4	0.0	0.9498415830929924	0.0
62-63	0.0	1.522751429102216E-4	0.0	1.071703996071978	0.0
64-65	0.0	1.522751429102216E-4	0.0	1.196485015956743	0.0
66-67	0.0	1.522751429102216E-4	0.0	1.3442849616114365	0.0
68-69	0.0	1.8611406355693755E-4	0.0	1.5151292122265434	0.0
70-71	0.0	1.8611406355693755E-4	0.0	1.700955644957984	0.0
72-73	0.0	1.8611406355693755E-4	0.0	1.8920440298499888	0.0
74-75	0.0	1.8611406355693755E-4	0.0	2.0893249372203426	8.45973016167898E-6
76-77	0.0	1.8611406355693755E-4	0.0	2.3049042409304077	1.691946032335796E-5
78-79	0.0	1.8611406355693755E-4	0.0	2.5496696136982657	1.691946032335796E-5
80-81	0.0	1.8611406355693755E-4	0.0	2.8177669222520345	1.691946032335796E-5
82-83	0.0	1.8611406355693755E-4	0.0	3.0986553428102614	1.691946032335796E-5
84-85	0.0	1.8611406355693755E-4	0.0	3.3838413062906216	1.691946032335796E-5
86-87	0.0	1.8611406355693755E-4	0.0	3.687926306952172	1.691946032335796E-5
88	0.0	1.8611406355693755E-4	0.0	3.932624001878737	1.691946032335796E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7630	0.0	48.857704	1
GTATCAA	13520	0.0	36.36967	1
ATCAACG	15770	0.0	30.461557	3
TCAACGC	15725	0.0	30.399275	4
TATCAAC	17110	0.0	28.433037	2
GTACATG	30005	0.0	24.879475	1
GTGGTAT	3610	0.0	24.74177	1
CAACGCA	19870	0.0	24.128773	5
TACATGG	29795	0.0	23.931787	2
ACGCAGA	19785	0.0	23.494368	7
CGCAGAG	19735	0.0	23.24409	8
AACGCAG	20620	0.0	23.181196	6
TAGACCG	190	3.6422352E-7	22.264956	5
ACATGGG	31570	0.0	21.901354	3
TGGTATC	4010	0.0	21.567825	2
GCAGAGT	21565	0.0	20.443405	9
TACCTGG	3845	0.0	19.437199	2
CATGGGA	14545	0.0	19.421988	4
GTTAGAC	780	0.0	18.078384	3
CATGGGG	14530	0.0	17.953962	4
>>END_MODULE