FastQCFastQC Report
Wed 25 May 2016
SRR2431435_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431435_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5910354
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT525760.889557545960868No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT449030.7597345268997423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329820.5580376403849922No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115320.19511521644896396No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106370.1799722994595586No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64990.10995957264150337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161950.061.792041
GTATCAA312700.043.0463141
GTGGTAT53050.041.860331
TGGTATC56850.038.714192
ATCAACG411100.032.6140373
TCAACGC416750.032.205734
TATCAAC417200.032.170992
CAACGCA448250.029.949065
AACGCAG463450.028.9759676
ACGCAGA499500.026.6588657
CGCAGAG500400.026.5169878
GCAGAGT528550.024.8112539
GTACATG309000.024.498731
TACATGG307350.023.777852
TCGATTA10900.023.3084431
ACATGGG317250.022.3391363
GAGTACT365550.021.75294312-13
CAGAGTA523850.021.6376910-11
AGAGTAC517550.021.02013210-11
AGTACTT367750.020.99022712-13
Produced by FastQC (version 0.11.5)