##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431435_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5910354
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26228361279206	33.0	31.0	34.0	30.0	34.0
2	32.36867825514343	34.0	31.0	34.0	30.0	34.0
3	32.47452233825588	34.0	31.0	34.0	30.0	34.0
4	36.03239044564843	37.0	35.0	37.0	35.0	37.0
5	35.94837111279629	37.0	35.0	37.0	35.0	37.0
6	35.99325438036368	37.0	35.0	37.0	35.0	37.0
7	35.93148769092342	37.0	35.0	37.0	35.0	37.0
8	35.91518562170726	37.0	35.0	37.0	35.0	37.0
9	37.58739950263554	39.0	37.0	39.0	35.0	39.0
10-11	37.527231363806635	39.0	37.0	39.0	34.5	39.0
12-13	37.50535991583584	39.0	37.0	39.0	35.0	39.0
14-15	38.82110690831716	40.0	38.0	41.0	34.5	41.0
16-17	38.87228996774135	40.0	38.0	41.0	35.0	41.0
18-19	38.95880576696422	40.0	38.5	41.0	35.0	41.0
20-21	38.98920699166243	40.0	39.0	41.0	35.0	41.0
22-23	38.94976739125947	40.0	38.5	41.0	35.0	41.0
24-25	38.89561767366219	40.5	38.0	41.0	35.0	41.0
26-27	38.7531185103295	40.0	38.0	41.0	34.5	41.0
28-29	38.66820650675069	40.0	38.0	41.0	34.0	41.0
30-31	38.48896834267457	40.0	38.0	41.0	34.0	41.0
32-33	38.28958206225887	40.0	38.0	41.0	34.0	41.0
34-35	38.159492392503054	40.0	37.0	41.0	34.0	41.0
36-37	38.026542064993066	40.0	37.0	41.0	33.0	41.0
38-39	37.933448148791086	40.0	37.0	41.0	33.0	41.0
40-41	37.81421908061682	40.0	37.0	41.0	33.0	41.0
42-43	37.68287550965644	40.0	37.0	41.0	33.0	41.0
44-45	37.46389564821329	40.0	37.0	41.0	33.0	41.0
46-47	37.14768920778688	40.0	36.5	41.0	31.5	41.0
48-49	36.89342211989333	40.0	36.0	41.0	31.0	41.0
50-51	36.737327408815105	40.0	36.0	41.0	31.0	41.0
52-53	36.576944206726026	40.0	35.5	41.0	31.0	41.0
54-55	36.400738686718256	39.0	35.0	41.0	30.5	41.0
56-57	36.14956286882308	39.0	35.0	41.0	30.0	41.0
58-59	35.9512183195795	39.0	35.0	41.0	30.0	41.0
60-61	35.72775750826431	38.5	35.0	41.0	29.0	41.0
62-63	35.491361769531906	38.0	35.0	41.0	29.0	41.0
64-65	35.23714552461663	37.0	35.0	40.0	29.0	41.0
66-67	34.96314721588588	37.0	35.0	40.0	29.0	41.0
68-69	34.671210303139205	36.5	34.5	39.5	28.0	41.0
70-71	34.35115561605954	36.0	34.0	39.0	28.0	41.0
72-73	34.03532114996834	35.5	34.0	39.0	28.0	41.0
74-75	33.730112189557516	35.0	34.0	38.5	27.5	40.5
76-77	33.39892339782016	35.0	34.0	37.0	27.0	39.5
78-79	33.10467799390696	35.0	34.0	37.0	26.5	39.0
80-81	32.822557836637195	35.0	34.0	36.5	26.0	39.0
82-83	32.600562758169815	35.0	33.5	36.0	26.0	38.0
84-85	32.39919004851486	35.0	33.5	36.0	26.0	37.0
86-87	32.18424065631263	35.0	33.0	35.0	26.0	37.0
88-89	32.05612870227401	35.0	33.0	35.0	26.0	36.5
90-91	31.885828158516393	35.0	33.0	35.0	26.0	36.0
92-93	31.710198238548827	35.0	33.0	35.0	25.0	36.0
94-95	31.56644238230062	35.0	33.0	35.0	25.0	36.0
96-97	31.414217743979464	35.0	33.0	35.0	25.0	36.0
98-99	31.261083177082117	35.0	33.0	35.0	24.0	35.0
100	31.15622871320398	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15616144469862547
1101	2	0.21680155009817526
1101	3	0.1830864367722782
1101	4	0.16051463330269655
1101	5	0.15904473546557085
1101	6	0.16767904352894192
1101	7	0.16328463785436753
1101	8	0.2234496445104952
1101	9	0.3307411201865591
1101	10-11	0.30257025494112355
1101	12-13	0.2919612556320246
1101	14-15	0.37252562961989355
1101	16-17	0.3785089824250605
1101	18-19	0.3712346336994159
1101	20-21	0.31820468073554053
1101	22-23	0.3175569520988759
1101	24-25	0.31159385621006663
1101	26-27	0.25057306168958604
1101	28-29	0.302431714376425
1101	30-31	0.30790497989139
1101	32-33	0.2952041553014766
1101	34-35	0.2910169298548837
1101	36-37	0.3292888241098737
1101	38-39	0.3304173176557299
1101	40-41	0.3867276525976493
1101	42-43	0.3521174972498571
1101	44-45	0.3019941121665113
1101	46-47	0.2595083201384085
1101	48-49	0.2556440981447281
1101	50-51	0.1646649002479066
1101	52-53	0.21711415864342598
1101	54-55	0.18599902639277133
1101	56-57	0.19342317050035973
1101	58-59	0.23242506117775008
1101	60-61	0.31218831726964424
1101	62-63	0.3192518058499232
1101	64-65	0.2685559852546433
1101	66-67	0.19920333812113
1101	68-69	0.22996817868628483
1101	70-71	0.28168468363813304
1101	72-73	0.3207566128069601
1101	74-75	0.2600186733455203
1101	76-77	0.27024974086383224
1101	78-79	0.2799064982966257
1101	80-81	0.28688965275286904
1101	82-83	0.2584091950899321
1101	84-85	0.2527222721407867
1101	86-87	0.2693571089556883
1101	88-89	0.24314032997771307
1101	90-91	0.2640271234040483
1101	92-93	0.3041927632299952
1101	94-95	0.2733617109050961
1101	96-97	0.3097543009785184
1101	98-99	0.3655382659634121
1101	100	0.3858547395813723
1102	1	0.15505089082111567
1102	2	0.2381431382959036
1102	3	0.21371827526549225
1102	4	0.1590972886603481
1102	5	0.14407301531472427
1102	6	0.15059796486912802
1102	7	0.1767595124255692
1102	8	0.20045747866841168
1102	9	0.2207766855478681
1102	10-11	0.28496282474341683
1102	12-13	0.269428017038166
1102	14-15	0.40574258066598645
1102	16-17	0.39127147385064376
1102	18-19	0.3455318386800883
1102	20-21	0.3318390475664117
1102	22-23	0.35050575033337594
1102	24-25	0.3133729147468216
1102	26-27	0.35324992016855106
1102	28-29	0.309312131850767
1102	30-31	0.31937265707338014
1102	32-33	0.3344128289400956
1102	34-35	0.3738484089470546
1102	36-37	0.34613038259173123
1102	38-39	0.3858788388214833
1102	40-41	0.4386485422118156
1102	42-43	0.42026724211786615
1102	44-45	0.46237766503134026
1102	46-47	0.4839921861763301
1102	48-49	0.44219274741926284
1102	50-51	0.42628341109917045
1102	52-53	0.4639848211141526
1102	54-55	0.4936162950327443
1102	56-57	0.5612647046825359
1102	58-59	0.5210077765526933
1102	60-61	0.48041930503987373
1102	62-63	0.4961488422552449
1102	64-65	0.5186038892902332
1102	66-67	0.5198656844220082
1102	68-69	0.5272561149496866
1102	70-71	0.5082587026557093
1102	72-73	0.4919553671995658
1102	74-75	0.5214776699795749
1102	76-77	0.5005619434306467
1102	78-79	0.5061170102205921
1102	80-81	0.47770795322161774
1102	82-83	0.41337754256245773
1102	84-85	0.4717131664948653
1102	86-87	0.48721638483112173
1102	88-89	0.42672666541925963
1102	90-91	0.45165043329550514
1102	92-93	0.4839386515340287
1102	94-95	0.5108860142435496
1102	96-97	0.4745752972477888
1102	98-99	0.5480943169786876
1102	100	0.5470334847986535
1103	1	0.14105536035074806
1103	2	0.20735193504646077
1103	3	0.14899440191226887
1103	4	0.19235438383046954
1103	5	0.13965133944109454
1103	6	0.13708801767123902
1103	7	0.15419470043838857
1103	8	0.15124683438131825
1103	9	0.23889566704282572
1103	10-11	0.25208328604681185
1103	12-13	0.27806798319607395
1103	14-15	0.39225764922660744
1103	16-17	0.34421444795602696
1103	18-19	0.3111621863322398
1103	20-21	0.33044163100037593
1103	22-23	0.2954504854342801
1103	24-25	0.3388507566561998
1103	26-27	0.35303544651327456
1103	28-29	0.33461347566315425
1103	30-31	0.37766957284742375
1103	32-33	0.3300870669718421
1103	34-35	0.36168959738276385
1103	36-37	0.4035500316551648
1103	38-39	0.41034719701839606
1103	40-41	0.43230063869962976
1103	42-43	0.4578385414776349
1103	44-45	0.43640305214753283
1103	46-47	0.44640719398032047
1103	48-49	0.38963358550623894
1103	50-51	0.3543359972837976
1103	52-53	0.39811688424951086
1103	54-55	0.37544318982700275
1103	56-57	0.4388366075519414
1103	58-59	0.4417337132256023
1103	60-61	0.4517547259376613
1103	62-63	0.4194930712620888
1103	64-65	0.41555216602473877
1103	66-67	0.4520533505784172
1103	68-69	0.41024722819933146
1103	70-71	0.40737417674916543
1103	72-73	0.3852819599187498
1103	74-75	0.3972912034408793
1103	76-77	0.41712146460269395
1103	78-79	0.4103155227544093
1103	80-81	0.3842425177050899
1103	82-83	0.3606440745449504
1103	84-85	0.3666223493278906
1103	86-87	0.3668909707349144
1103	88-89	0.3918383933406133
1103	90-91	0.41374686203442934
1103	92-93	0.40043586460773284
1103	94-95	0.4559787301884377
1103	96-97	0.44628187656307716
1103	98-99	0.5264258591172855
1103	100	0.502981196668074
1104	1	0.09787268958985607
1104	2	0.14034120426930485
1104	3	0.11243369977835016
1104	4	0.1311572481768266
1104	5	0.08851605408127483
1104	6	0.10221926072168941
1104	7	0.1237313471089152
1104	8	0.1279188160098812
1104	9	0.22528396844882792
1104	10-11	0.22644612026307698
1104	12-13	0.21585561001118947
1104	14-15	0.3064254608497379
1104	16-17	0.24039333942166508
1104	18-19	0.23338414252100392
1104	20-21	0.24250971371251495
1104	22-23	0.23141967229758365
1104	24-25	0.27716586844658764
1104	26-27	0.25434593699870334
1104	28-29	0.2556837094905404
1104	30-31	0.2913732918626266
1104	32-33	0.26397843816462085
1104	34-35	0.3075657436924004
1104	36-37	0.27250918504353905
1104	38-39	0.26360732245436935
1104	40-41	0.2946654393910393
1104	42-43	0.3150499196605665
1104	44-45	0.3667189336491248
1104	46-47	0.42075411451692446
1104	48-49	0.3778442414221388
1104	50-51	0.28080753420751137
1104	52-53	0.2448638478227707
1104	54-55	0.29394410656541226
1104	56-57	0.3226004193831784
1104	58-59	0.3979533301793907
1104	60-61	0.3471332318075966
1104	62-63	0.31803808870762396
1104	64-65	0.3245099217708898
1104	66-67	0.32016275489618096
1104	68-69	0.32190446181662224
1104	70-71	0.34033913014562955
1104	72-73	0.3744102379572638
1104	74-75	0.34018296523337455
1104	76-77	0.32655087972159436
1104	78-79	0.3478373393391223
1104	80-81	0.37534556257126184
1104	82-83	0.34341199656551424
1104	84-85	0.35471566515714414
1104	86-87	0.3723937278541314
1104	88-89	0.3521455062321053
1104	90-91	0.33146165680499706
1104	92-93	0.38789289560141427
1104	94-95	0.3187075295494246
1104	96-97	0.3378409681359571
1104	98-99	0.34892665749382346
1104	100	0.4286385883589503
1105	1	0.08264810022206603
1105	2	0.11641128432128056
1105	3	0.13103581348496363
1105	4	0.0949017685243092
1105	5	0.08103132313502925
1105	6	0.12165301120010241
1105	7	0.14996645998252234
1105	8	0.13990212859852846
1105	9	0.1723338007224129
1105	10-11	0.20659884296291864
1105	12-13	0.1095916596119011
1105	14-15	0.20214433658424014
1105	16-17	0.15831751196598987
1105	18-19	0.23350495217484024
1105	20-21	0.20351852859594288
1105	22-23	0.21355369342178676
1105	24-25	0.20829379240203139
1105	26-27	0.23149226824470048
1105	28-29	0.28916092893071976
1105	30-31	0.20729956219322077
1105	32-33	0.2216338303356764
1105	34-35	0.2684449645927458
1105	36-37	0.2725363621902872
1105	38-39	0.27822603432193915
1105	40-41	0.3165031612707381
1105	42-43	0.33158532392464934
1105	44-45	0.34327771990810163
1105	46-47	0.3503154327532698
1105	48-49	0.3489818904542119
1105	50-51	0.32493887862397486
1105	52-53	0.32992475920597997
1105	54-55	0.31442940356820515
1105	56-57	0.3324448382585885
1105	58-59	0.33718830555915247
1105	60-61	0.30971558740689176
1105	62-63	0.2983438436694641
1105	64-65	0.32413183378768906
1105	66-67	0.2835478011938122
1105	68-69	0.26365077038820317
1105	70-71	0.31220475282391646
1105	72-73	0.2785946541953592
1105	74-75	0.2641757624854293
1105	76-77	0.31731627822862407
1105	78-79	0.26687625733489995
1105	80-81	0.24046220720674683
1105	82-83	0.24082932336347085
1105	84-85	0.2983515693842378
1105	86-87	0.2578468183299094
1105	88-89	0.2459140196582581
1105	90-91	0.27407081968455316
1105	92-93	0.3100367825123236
1105	94-95	0.33018350189627554
1105	96-97	0.3470702628487956
1105	98-99	0.36536032837053867
1105	100	0.41832679090427405
1106	1	0.10015541191194188
1106	2	0.1882863138940678
1106	3	0.1267458566440567
1106	4	0.10351542597411623
1106	5	0.058963209984639775
1106	6	0.0791543882388126
1106	7	0.08430651912421183
1106	8	0.1460051356310501
1106	9	0.18106993651262826
1106	10-11	0.1587178102921314
1106	12-13	0.1573892921553579
1106	14-15	0.2061481649398189
1106	16-17	0.19761649847328044
1106	18-19	0.19170963982331557
1106	20-21	0.1332896063625597
1106	22-23	0.17651020492545655
1106	24-25	0.21281661753970127
1106	26-27	0.249927319658255
1106	28-29	0.24182082713939934
1106	30-31	0.21409630635388055
1106	32-33	0.23598386352849587
1106	34-35	0.23070187950492027
1106	36-37	0.2704970809701379
1106	38-39	0.2511554367331499
1106	40-41	0.2451858566809264
1106	42-43	0.2348301638050927
1106	44-45	0.24425400761916194
1106	46-47	0.20330589155054213
1106	48-49	0.18713939645918742
1106	50-51	0.21446932041585143
1106	52-53	0.1699741524079883
1106	54-55	0.2228171605565521
1106	56-57	0.26443535629866943
1106	58-59	0.22968946286798086
1106	60-61	0.22456451112588383
1106	62-63	0.17838218167157294
1106	64-65	0.17371167655961983
1106	66-67	0.18173762888022083
1106	68-69	0.206260813897309
1106	70-71	0.23118500008030196
1106	72-73	0.254691342859644
1106	74-75	0.2525331319337951
1106	76-77	0.28962561459643155
1106	78-79	0.22508559884918355
1106	80-81	0.22414273967121545
1106	82-83	0.18926811990747439
1106	84-85	0.2067464368054459
1106	86-87	0.21246283745458072
1106	88-89	0.20800847705963577
1106	90-91	0.1766439732049534
1106	92-93	0.19907946935110132
1106	94-95	0.26938457573368524
1106	96-97	0.23958542064530874
1106	98-99	0.2715848609324958
1106	100	0.32780543218805747
1107	1	0.06418959659113455
1107	2	0.10540757803883594
1107	3	0.02268877859729912
1107	4	0.0566713317043579
1107	5	0.07901948384029112
1107	6	0.033474433923430524
1107	7	-0.03674144860262629
1107	8	-0.0046195052679181
1107	9	0.05725395161515934
1107	10-11	0.04890688154725353
1107	12-13	-0.03615966835906903
1107	14-15	0.08706556248510111
1107	16-17	0.040972543973396114
1107	18-19	0.013399152179715657
1107	20-21	0.03854319523304639
1107	22-23	0.06099758327722071
1107	24-25	0.05288219195107047
1107	26-27	0.10459402947272878
1107	28-29	0.11623293508591104
1107	30-31	0.11923581881916334
1107	32-33	0.11752641629265526
1107	34-35	0.08834881663477745
1107	36-37	0.08535850228957287
1107	38-39	0.059916035383501764
1107	40-41	0.055059344746204886
1107	42-43	0.10308086945575212
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2216	76-77	-1.0344897075167694
2216	78-79	-0.9946726031501072
2216	80-81	-1.0332215367478952
2216	82-83	-1.0467997733150582
2216	84-85	-1.0937711893153619
2216	86-87	-1.112590286326764
2216	88-89	-1.0837994194268603
2216	90-91	-1.1143842698584976
2216	92-93	-1.0144741915282758
2216	94-95	-1.1301806225868134
2216	96-97	-1.321659249218314
2216	98-99	-1.3757239418458873
2216	100	-1.480098135407271
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	331.0
3	1010.0
4	822.0
5	517.0
6	689.0
7	1385.0
8	2605.0
9	4257.0
10	5907.0
11	7421.0
12	9528.0
13	12981.0
14	15813.0
15	21818.0
16	28885.0
17	42193.0
18	47481.0
19	27945.0
20	19768.0
21	18898.0
22	19176.0
23	20593.0
24	23093.0
25	28378.0
26	34938.0
27	45135.0
28	57552.0
29	73873.0
30	93620.0
31	118371.0
32	150375.0
33	190557.0
34	254532.0
35	390113.0
36	646529.0
37	1217905.0
38	1568121.0
39	703312.0
40	3927.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.303160294494525	21.378597179842824	15.20849195631265	24.109750569349995
2	14.365755418372572	21.45478934087535	38.923100037662714	25.25635520308936
3	16.57802552404698	27.350775284297978	31.039999675146028	25.031199516509012
4	11.532275132275133	18.690116402116402	38.033726984126986	31.743881481481484
5	12.058272527737302	38.77812731172374	34.79307936323157	14.370520797307385
6	28.534822110486108	38.99757950200614	17.696148149501706	14.771450238006048
7	25.48094411942161	34.30586391271995	22.14718441568813	18.06600755217031
8	25.741656083544235	35.24753339647676	19.44003692503021	19.570773594948797
9	26.15017645305171	16.214071103016842	21.490963147046692	36.14478929688475
10-11	24.095721940561962	27.445908637840443	27.635630604292043	20.82273881730555
12-13	25.09453748455676	25.50764302781187	28.218859648677558	21.17895983895381
14-15	22.37260238557623	26.682581111046815	26.88816947343594	24.056647029941015
16-17	20.2371127008636	29.78688755360508	27.98453358292921	21.991466162602105
18-19	20.174815247953	27.433179129371947	31.76890927345469	20.62309634922037
20-21	21.536518793967332	26.357981264743195	31.72926697791706	20.376232963372416
22-23	21.355641303380473	26.55416240719253	31.62321580061025	20.466980488816745
24-25	20.824344117910307	26.560688815145973	31.87041554243313	20.74455152451059
26-27	20.56759205962959	26.806786869280586	31.87402142071355	20.75159965037627
28-29	20.521418439668313	26.706790701278667	32.32850667884645	20.443284180206568
30-31	20.897373119499274	26.78002912965675	31.776266097790103	20.546331653053876
32-33	20.557059134649062	26.916885689525245	31.87133671790905	20.654718457916648
34-35	21.07725159335382	26.458509774580737	31.746167909718253	20.71807072234719
36-37	20.742057320219505	26.65476161470135	31.60401286205842	20.99916820302073
38-39	20.684579843467322	26.79484473418504	31.628906813439496	20.89166860890814
40-41	20.880612505253556	26.448153760462272	31.57118168940782	21.100052044876357
42-43	20.789045034265147	26.897788970417917	31.569795979278652	20.743370016038284
44-45	21.026290646260893	26.701941491481136	31.198136558002542	21.07363130425543
46-47	21.013079481571744	26.63968958734256	30.908938017970584	21.438292913115113
48-49	20.98755760793602	26.89943020837202	30.37777913322269	21.735233050469276
50-51	21.18503784563738	26.97485228437978	30.050284602610866	21.789825267371977
52-53	21.270418657290737	27.254866346723013	29.90058984200748	21.57412515397877
54-55	21.214945767875797	27.373731417679526	29.895985618976127	21.515337195468547
56-57	21.287078705298615	27.18435004016923	29.870578592608183	21.657992661923966
58-59	21.50932659333586	26.733975267667937	30.085140862343014	21.67155727665319
60-61	21.189521355038384	27.172541075971512	29.993445305695104	21.644492263295
62-63	21.068297633031722	26.932737865358984	30.256442360851732	21.742522140757565
64-65	21.216528784109933	27.055851043355194	30.03501439482556	21.69260577770931
66-67	21.126301235156113	26.915285501051883	30.154884333336827	21.803528930455172
68-69	21.300002241849924	27.061598845066598	30.067733477037322	21.570665436046156
70-71	21.274019477919477	27.023568128600527	29.90750860336392	21.794903790116074
72-73	21.275223108463553	26.952438043474213	29.930508392559908	21.841830455502325
74-75	21.305931638450577	27.035204404484343	29.54998358808461	22.108880368980465
76-77	21.421432624847853	26.71952475266287	29.43876119772183	22.42028142476745
78-79	21.389011085694175	26.764752162319468	29.69742755045412	22.148809201532234
80-81	20.927976564517117	27.162044777690138	29.685167758141052	22.224810899651697
82-83	21.130465613186107	27.10820119143225	29.846827092753536	21.914506102628106
84-85	21.137687150542135	26.822561333654694	29.647072140226694	22.39267937557648
86-87	21.346631690758286	26.924309440686635	29.459056090379697	22.270002778175385
88-89	21.18622674716269	26.93201625486392	29.45685656053766	22.424900437435728
90-91	21.019553143517292	27.05616279498656	29.27828857628494	22.645995485211206
92-93	20.994373761387454	27.050807372770073	29.474143886403887	22.480674979438586
94-95	20.907910084573615	26.870810107144173	29.64313135896767	22.578148449314543
96-97	21.154274348225684	27.22005445383065	29.144846106679694	22.48082509126397
98-99	20.81156560637949	27.154990163810165	29.391215395045645	22.6422288347647
100	20.816961598987806	26.965745958057568	29.513907747085234	22.703384695869392
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2769.0
1	1782.0
2	2472.5
3	4693.5
4	3304.5
5	2142.5
6	6696.0
7	18120.5
8	29626.0
9	31886.5
10	21393.5
11	9875.5
12	7185.5
13	7293.0
14	7605.5
15	8119.5
16	8713.5
17	9288.0
18	9964.0
19	10743.5
20	11974.5
21	13718.5
22	16779.5
23	21811.5
24	28068.0
25	35655.0
26	45637.0
27	57648.5
28	72760.0
29	88211.0
30	98836.5
31	107904.5
32	117863.5
33	127406.0
34	136163.0
35	144714.0
36	150395.5
37	156307.0
38	164410.5
39	168574.5
40	175156.5
41	183324.5
42	189167.0
43	202479.5
44	211083.5
45	210416.0
46	211403.5
47	213345.5
48	213188.5
49	212545.5
50	218060.5
51	210947.5
52	190420.5
53	173652.0
54	164102.5
55	156628.0
56	134456.0
57	109712.5
58	97540.5
59	93645.0
60	80191.5
61	60425.5
62	50582.0
63	42173.0
64	33620.5
65	26607.0
66	20716.5
67	15973.5
68	11937.5
69	8942.5
70	6546.5
71	4471.5
72	3099.0
73	2254.0
74	1587.0
75	1038.5
76	676.0
77	479.0
78	294.5
79	168.0
80	82.0
81	33.0
82	15.5
83	6.0
84	4.5
85	7.0
86	4.5
87	1.0
88	1.0
89	1.0
90	1.5
91	2.0
92	1.0
93	0.5
94	1.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0463593212860008
2	0.0
3	1.0151676194014776E-4
4	0.06943746516706106
5	0.004432898604719785
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.011056867321314424
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.245032700240967E-4
26-27	0.0
28-29	2.453321746886904E-4
30-31	0.0038237980330788988
32-33	2.0303352388029551E-4
34-35	0.0017004057624974748
36-37	0.004365220763426353
38-39	0.0014635333179704633
40-41	0.001184362222635057
42-43	0.002047254699126313
44-45	1.691946032335796E-5
46-47	4.737448890540228E-4
48-49	6.006408414792075E-4
50-51	0.0016158084608806848
52-53	7.190770637427132E-4
54-55	0.0011082246511799462
56-57	0.006057166795762149
58-59	0.009466438050918777
60-61	0.0014550735878087844
62-63	0.0026478955406055204
64-65	0.001192821952796736
66-67	1.3535568258686367E-4
68-69	9.559495082697247E-4
70-71	3.4684893662883815E-4
72-73	0.0
74-75	2.368724445270114E-4
76-77	0.0
78-79	5.752616509941707E-4
80-81	0.0
82-83	0.001226660873443452
84-85	6.513992224492813E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	1.8611406355693752E-4
94-95	0.0
96-97	4.399059684073069E-4
98-99	6.260200319642444E-4
100	2.5379190485036934E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5910354.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.12708056740298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.28978862771638	22.50348212280012
2	17.679445265059094	12.77413486955562
3	7.523686436924519	8.154264782119474
4	3.9390427754314166	5.6922446282582975
5	2.2996586400535275	4.153997648336908
6	1.4512447294560542	3.145754115844669
7	0.965990533208724	2.4428892474406094
8	0.6890073837009026	1.9913460209998437
9	0.5115796635143943	1.6633691748386488
>10	2.341695950699892	14.747692907982504
>50	0.1672760569510057	4.235464669283555
>100	0.12340545972716187	8.680960199376903
>500	0.010991648124835086	2.745799685506446
>1k	0.006811047501124308	4.182040288238626
>5k	1.409182241577567E-4	0.2964468359072719
>10k+	2.3486370692959448E-4	2.590112803510613
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52576	0.889557545960868	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44903	0.7597345268997423	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32982	0.5580376403849922	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11532	0.19511521644896396	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10637	0.1799722994595586	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6499	0.10995957264150337	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.691946032335796E-5	0.0
3	0.0	0.0	0.0	1.691946032335796E-5	0.0
4	0.0	0.0	0.0	1.691946032335796E-5	0.0
5	0.0	0.0	0.0	3.383892064671592E-5	0.0
6	0.0	0.0	0.0	3.383892064671592E-5	0.0
7	0.0	0.0	0.0	3.383892064671592E-5	0.0
8	0.0	0.0	0.0	3.383892064671592E-5	0.0
9	0.0	0.0	0.0	3.383892064671592E-5	0.0
10-11	0.0	0.0	0.0	7.61375714551108E-5	0.0
12-13	0.0	0.0	0.0	1.1843622226350571E-4	0.0
14-15	0.0	0.0	0.0	1.1843622226350571E-4	0.0
16-17	0.0	0.0	0.0	1.3535568258686367E-4	0.0
18-19	0.0	8.45973016167898E-6	0.0	1.6919460323357958E-4	0.0
20-21	0.0	1.691946032335796E-5	0.0	1.9457379371861652E-4	0.0
22-23	0.0	1.691946032335796E-5	0.0	4.6528515889234385E-4	0.0
24-25	0.0	1.691946032335796E-5	0.0	0.0010490065400481936	0.0
26-27	0.0	1.691946032335796E-5	0.0	0.0018188419847609805	0.0
28-29	0.0	3.383892064671592E-5	0.0	0.004534415366659932	0.0
30-31	0.0	5.075838097007387E-5	0.0	0.011572910861176844	0.0
32-33	0.0	5.075838097007387E-5	0.0	0.024753170453072692	0.0
34-35	0.0	5.075838097007387E-5	0.0	0.04554718719047962	0.0
36-37	0.0	5.921811113175285E-5	0.0	0.07851475563054261	0.0
38-39	0.0	6.767784129343183E-5	0.0	0.12143942647090175	0.0
40-41	0.0	6.767784129343183E-5	0.0	0.16535388574017731	0.0
42-43	0.0	6.767784129343183E-5	0.0	0.2093021839300996	0.0
44-45	8.45973016167898E-6	6.767784129343183E-5	0.0	0.2660077552038338	0.0
46-47	1.691946032335796E-5	8.459730161678979E-5	0.0	0.33555519686299673	0.0
48-49	1.691946032335796E-5	8.459730161678979E-5	0.0	0.40910409088863375	0.0
50-51	1.691946032335796E-5	8.459730161678979E-5	0.0	0.48354971631140875	0.0
52-53	1.691946032335796E-5	8.459730161678979E-5	0.0	0.556066523257321	0.0
54-55	1.691946032335796E-5	8.459730161678979E-5	0.0	0.6382105031272238	0.0
56-57	1.691946032335796E-5	8.459730161678979E-5	0.0	0.7369609333045026	0.0
58-59	1.691946032335796E-5	8.459730161678979E-5	0.0	0.850397455042456	0.0
60-61	1.691946032335796E-5	8.459730161678979E-5	0.0	0.9711854822909085	0.0
62-63	1.691946032335796E-5	8.459730161678979E-5	0.0	1.0957465491914697	0.0
64-65	1.691946032335796E-5	8.459730161678979E-5	0.0	1.2236661289662174	0.0
66-67	1.691946032335796E-5	8.459730161678979E-5	0.0	1.3744946580187922	0.0
68-69	1.691946032335796E-5	8.459730161678979E-5	0.0	1.5484605490635586	0.0
70-71	1.691946032335796E-5	1.0151676194014776E-4	0.0	1.7369856357165747	0.0
72-73	1.691946032335796E-5	1.1843622226350571E-4	0.0	1.931982415943275	0.0
74-75	1.691946032335796E-5	1.1843622226350571E-4	0.0	2.133357832711882	0.0
76-77	1.691946032335796E-5	1.1843622226350571E-4	0.0	2.3531331625821394	0.0
78-79	1.691946032335796E-5	1.1843622226350571E-4	0.0	2.603397359955089	0.0
80-81	1.691946032335796E-5	1.1843622226350571E-4	0.0	2.877577214495105	0.0
82-83	1.691946032335796E-5	1.1843622226350571E-4	0.0	3.1642097918331116	0.0
84-85	1.691946032335796E-5	1.1843622226350571E-4	0.0	3.456265056204755	0.0
86-87	1.691946032335796E-5	1.1843622226350571E-4	0.0	3.7685559951231347	0.0
88	1.691946032335796E-5	1.1843622226350571E-4	0.0	4.019843819845647	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16195	0.0	61.79204	1
GTATCAA	31270	0.0	43.046314	1
GTGGTAT	5305	0.0	41.86033	1
TGGTATC	5685	0.0	38.71419	2
ATCAACG	41110	0.0	32.614037	3
TCAACGC	41675	0.0	32.20573	4
TATCAAC	41720	0.0	32.17099	2
CAACGCA	44825	0.0	29.94906	5
AACGCAG	46345	0.0	28.975967	6
ACGCAGA	49950	0.0	26.658865	7
CGCAGAG	50040	0.0	26.516987	8
GCAGAGT	52855	0.0	24.811253	9
GTACATG	30900	0.0	24.49873	1
TACATGG	30735	0.0	23.77785	2
TCGATTA	1090	0.0	23.308443	1
ACATGGG	31725	0.0	22.339136	3
GAGTACT	36555	0.0	21.752943	12-13
CAGAGTA	52385	0.0	21.63769	10-11
AGAGTAC	51755	0.0	21.020132	10-11
AGTACTT	36775	0.0	20.990227	12-13
>>END_MODULE