FastQCFastQC Report
Wed 25 May 2016
SRR2431434_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431434_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5043176
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA376230.7460179854916822No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65350.044.2407231
GTATCAA112250.035.4304541
TACCTGG42800.032.2888952
ATCAACG125950.030.192563
TCAACGC125650.030.189834
TATCAAC140400.027.955612
GTACCTG49700.027.9034791
CAACGCA139500.027.259615
AACGCAG147950.025.7959756
GTACATG204150.025.4221571
TACATGG203050.024.5156062
TCTGTCG12500.024.0642438
CATGGGT78900.023.7708954
ACGCAGA159500.023.574147
GTCTCGC14000.023.5051331
CGCAGAG158400.023.4705668
ACATGGG211950.022.4659943
ACCTGGG60850.022.2475153
GTGGTAT36100.021.6169071
CTGTCGC15600.021.3912439
Produced by FastQC (version 0.11.5)