##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431434_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5043176 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.102803868038713 31.0 31.0 34.0 26.0 34.0 2 30.099381818124133 31.0 31.0 34.0 26.0 34.0 3 30.202547957874167 31.0 31.0 34.0 26.0 34.0 4 33.48839005420394 37.0 35.0 37.0 27.0 37.0 5 33.66597695579135 37.0 35.0 37.0 28.0 37.0 6 33.74981301465584 37.0 35.0 37.0 28.0 37.0 7 33.64393925573884 37.0 35.0 37.0 28.0 37.0 8 33.57212637433236 37.0 35.0 37.0 28.0 37.0 9 34.92184964395452 39.0 35.0 39.0 27.0 39.0 10-11 34.871127638615036 39.0 35.0 39.0 27.0 39.0 12-13 34.841862350233264 39.0 35.0 39.0 27.0 39.0 14-15 36.043583349064164 40.0 36.5 41.0 27.0 41.0 16-17 36.05899278549866 40.0 36.5 41.0 27.0 41.0 18-19 36.05351478909322 40.0 36.5 41.0 27.0 41.0 20-21 36.15492945318585 40.0 37.0 41.0 27.0 41.0 22-23 36.12135765239999 40.0 36.0 41.0 27.0 41.0 24-25 36.1593094311997 40.0 36.0 41.0 27.0 41.0 26-27 36.150419200123096 40.0 36.0 41.0 27.0 41.0 28-29 36.04151669503503 40.0 36.0 41.0 27.0 41.0 30-31 36.0214487854479 40.0 36.0 41.0 27.0 41.0 32-33 35.961278963891004 40.0 36.0 41.0 27.0 41.0 34-35 35.860801605972114 40.0 36.0 41.0 27.0 41.0 36-37 35.737467718755006 40.0 35.0 41.0 27.0 41.0 38-39 35.62849214463267 40.0 35.0 41.0 26.0 41.0 40-41 35.54517550051793 40.0 35.0 41.0 26.0 41.0 42-43 35.335894682239925 40.0 35.0 41.0 25.0 41.0 44-45 35.21274797865472 40.0 35.0 41.0 25.0 41.0 46-47 35.07977086264687 39.0 35.0 41.0 25.0 41.0 48-49 34.95100448606196 39.0 35.0 41.0 24.5 41.0 50-51 34.79653069811563 39.0 34.0 41.0 24.0 41.0 52-53 34.704992052627155 39.0 34.0 41.0 24.0 41.0 54-55 34.63446030834538 39.0 34.0 41.0 24.0 41.0 56-57 34.47954245499265 39.0 34.0 41.0 23.5 41.0 58-59 34.27987601463839 38.0 34.0 41.0 23.0 41.0 60-61 34.06608018439174 38.0 33.5 40.0 22.5 41.0 62-63 33.790377432792354 38.0 33.0 40.0 22.0 41.0 64-65 33.634245959292315 37.0 33.0 40.0 21.5 41.0 66-67 33.36993920101143 37.0 33.0 40.0 20.0 41.0 68-69 33.093563262515524 36.0 33.0 40.0 19.5 41.0 70-71 32.7826754410316 36.0 32.5 39.0 18.0 41.0 72-73 32.45821769852965 35.5 32.0 39.0 16.5 41.0 74-75 32.114872651678226 35.0 32.0 38.5 12.5 40.5 76-77 31.78428196834693 35.0 32.0 37.5 9.0 40.0 78-79 31.4394707819041 35.0 32.0 37.0 7.0 39.0 80-81 31.129628135127547 35.0 31.0 36.5 2.0 39.0 82-83 30.821982318285144 35.0 31.0 36.0 2.0 38.5 84-85 30.524208752579725 35.0 31.0 36.0 2.0 37.0 86-87 30.260925654785794 35.0 31.0 35.5 2.0 37.0 88-89 29.987497660204603 35.0 31.0 35.0 2.0 36.5 90-91 29.76387002952108 35.0 31.0 35.0 2.0 36.0 92-93 29.56622632246029 35.0 30.0 35.0 2.0 36.0 94-95 29.334303819656505 34.5 30.0 35.0 2.0 36.0 96-97 29.228156820225983 35.0 30.0 35.0 2.0 35.5 98-99 29.066691009792244 35.0 30.0 35.0 2.0 35.0 100 28.93364796310896 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.40754051246937806 1101 2 0.4531378446854042 1101 3 0.3743649590479059 1101 4 0.45658537044162273 1101 5 0.3429335582402544 1101 6 0.4388825897283226 1101 7 0.48685095919707067 1101 8 0.45322123287905924 1101 9 0.5976675390219768 1101 10-11 0.6755923960997023 1101 12-13 0.6275275971841552 1101 14-15 0.7574464075490539 1101 16-17 0.6738061288280051 1101 18-19 0.6986212471250397 1101 20-21 0.5951106351127322 1101 22-23 0.638548461641065 1101 24-25 0.6216089792482435 1101 26-27 0.6920529598044141 1101 28-29 0.7382850424594878 1101 30-31 0.6979316300374308 1101 32-33 0.6664340927141907 1101 34-35 0.6415001556491404 1101 36-37 0.6603849961864157 1101 38-39 0.6732461713297724 1101 40-41 0.6470989236986497 1101 42-43 0.6662720931032382 1101 44-45 0.6170145038754313 1101 46-47 0.6076094863840424 1101 48-49 0.6052104036658434 1101 50-51 0.5622969697122358 1101 52-53 0.5586700163633651 1101 54-55 0.5659372049350466 1101 56-57 0.48041193425444106 1101 58-59 0.4757070162728283 1101 60-61 0.4739076420134154 1101 62-63 0.476682639351786 1101 64-65 0.5167331853806303 1101 66-67 0.5265126349878528 1101 68-69 0.5038821856152182 1101 70-71 0.5323116854064338 1101 72-73 0.45869859193104645 1101 74-75 0.4922840491291822 1101 76-77 0.5570493622182795 1101 78-79 0.5416167844129767 1101 80-81 0.5490221819987546 1101 82-83 0.5021379376377268 1101 84-85 0.46198087448259884 1101 86-87 0.4597903608836731 1101 88-89 0.5217163663227637 1101 90-91 0.518693869737497 1101 92-93 0.4748995855712437 1101 94-95 0.46918544216609703 1101 96-97 0.5521709881846419 1101 98-99 0.5681583749324552 1101 100 0.5087891929641017 1102 1 0.24442064774705585 1102 2 0.33934936627746737 1102 3 0.3885041603606254 1102 4 0.354096535289699 1102 5 0.3236959000880475 1102 6 0.3709731273257191 1102 7 0.4466195347897184 1102 8 0.3983950931157807 1102 9 0.530181782325144 1102 10-11 0.5555593267816477 1102 12-13 0.5004255658092518 1102 14-15 0.585550553600612 1102 16-17 0.6070851609300973 1102 18-19 0.5512117108273387 1102 20-21 0.5304923118479863 1102 22-23 0.5202462199946112 1102 24-25 0.5817576735960728 1102 26-27 0.5907479056846299 1102 28-29 0.6685656978207177 1102 30-31 0.6287429818633896 1102 32-33 0.6638961158095924 1102 34-35 0.6696729967621309 1102 36-37 0.5903510551293536 1102 38-39 0.5558172803987134 1102 40-41 0.5867702253813718 1102 42-43 0.6170446160517713 1102 44-45 0.5135731823515215 1102 46-47 0.47888233076100306 1102 48-49 0.4554473443565783 1102 50-51 0.4529901725198755 1102 52-53 0.40391924147861147 1102 54-55 0.518355196087505 1102 56-57 0.5752922819931641 1102 58-59 0.575602194724901 1102 60-61 0.5896682272057348 1102 62-63 0.572104306607379 1102 64-65 0.6291216407989921 1102 66-67 0.6512458777819745 1102 68-69 0.640418582036709 1102 70-71 0.6403402597948613 1102 72-73 0.6674248818969843 1102 74-75 0.6872657314597745 1102 76-77 0.6111183763767443 1102 78-79 0.527750670550482 1102 80-81 0.5569339332230925 1102 82-83 0.528026974587096 1102 84-85 0.5176781957502534 1102 86-87 0.48320449023230694 1102 88-89 0.48745351592675945 1102 90-91 0.4025189145560333 1102 92-93 0.37679914542413684 1102 94-95 0.37752713304379526 1102 96-97 0.4279081499524402 1102 98-99 0.467070687921634 1102 100 0.4362178325099535 1103 1 0.17776156817868127 1103 2 0.2255463387731318 1103 3 0.22384852776148634 1103 4 0.2059751043724134 1103 5 0.19379745020786032 1103 6 0.28792282309134976 1103 7 0.3287978269512237 1103 8 0.3027415195511196 1103 9 0.36422495842946034 1103 10-11 0.46975798049321327 1103 12-13 0.376614747938568 1103 14-15 0.5069377958278665 1103 16-17 0.4292162480253836 1103 18-19 0.43760687952387656 1103 20-21 0.43254085399256326 1103 22-23 0.43224351219625845 1103 24-25 0.4619460365078609 1103 26-27 0.47507600372317427 1103 28-29 0.5079896668823736 1103 30-31 0.41235702798530127 1103 32-33 0.45473296329698343 1103 34-35 0.46721666581117205 1103 36-37 0.4646918767238546 1103 38-39 0.41632211630722793 1103 40-41 0.38726327056096466 1103 42-43 0.3563534845778733 1103 44-45 0.34869020270657813 1103 46-47 0.33910363530936394 1103 48-49 0.3176557517044998 1103 50-51 0.22242466661228377 1103 52-53 0.29975877182855726 1103 54-55 0.35488101965895424 1103 56-57 0.3098505574669943 1103 58-59 0.3144679211665178 1103 60-61 0.34003634525751636 1103 62-63 0.24773936859764234 1103 64-65 0.27315644824721375 1103 66-67 0.3028230921264452 1103 68-69 0.24124202840796016 1103 70-71 0.31694963953618327 1103 72-73 0.2810761477389505 1103 74-75 0.3126264780901238 1103 76-77 0.319939065550777 1103 78-79 0.29165180246597444 1103 80-81 0.2588000383499427 1103 82-83 0.24563171938346073 1103 84-85 0.2516199505499692 1103 86-87 0.21875532243181084 1103 88-89 0.2469494233647893 1103 90-91 0.23170188649176282 1103 92-93 0.20894257278115447 1103 94-95 0.23930178369750976 1103 96-97 0.27347930344231486 1103 98-99 0.26047004240374605 1103 100 0.29403673320086554 1104 1 0.1989136412135828 1104 2 0.24704667311237571 1104 3 0.22102871923978995 1104 4 0.2678470311013328 1104 5 0.2092446822981131 1104 6 0.254759577926869 1104 7 0.25659159263714315 1104 8 0.2444572888176424 1104 9 0.36389445252046215 1104 10-11 0.3521079751513412 1104 12-13 0.29233546460963566 1104 14-15 0.3747568302448414 1104 16-17 0.39081553312564665 1104 18-19 0.3134928613550869 1104 20-21 0.34452786467170426 1104 22-23 0.35883472650623816 1104 24-25 0.4066379514102749 1104 26-27 0.4662888471255826 1104 28-29 0.4187591361310794 1104 30-31 0.423804490151916 1104 32-33 0.43447276989781614 1104 34-35 0.4348864271294559 1104 36-37 0.37515651959076735 1104 38-39 0.42549950064036324 1104 40-41 0.4425517694168519 1104 42-43 0.3911474703086384 1104 44-45 0.39191769608377314 1104 46-47 0.38869527456642317 1104 48-49 0.4003790730977883 1104 50-51 0.37688381877099175 1104 52-53 0.3369902434060279 1104 54-55 0.3102990528013123 1104 56-57 0.33586184928815044 1104 58-59 0.3882445257955922 1104 60-61 0.2731380968701771 1104 62-63 0.3447308761024104 1104 64-65 0.3490382305746138 1104 66-67 0.38805498769740865 1104 68-69 0.35687085566352295 1104 70-71 0.3910247007274563 1104 72-73 0.3447983926327325 1104 74-75 0.39222898837881814 1104 76-77 0.3307282526332571 1104 78-79 0.33907014128338986 1104 80-81 0.3554854798373732 1104 82-83 0.305811000053847 1104 84-85 0.3659286517654934 1104 86-87 0.3565977893935184 1104 88-89 0.37970848821882086 1104 90-91 0.3212197742992551 1104 92-93 0.2946780941335341 1104 94-95 0.3567677413028143 1104 96-97 0.39379199826773004 1104 98-99 0.42617138261760346 1104 100 0.4219187380523799 1105 1 -0.022426171391124683 1105 2 0.04880146012596498 1105 3 0.11616429490188196 1105 4 0.07489264934369544 1105 5 0.03516332959848256 1105 6 0.13627451634652488 1105 7 0.20164758536946437 1105 8 0.16450949969737394 1105 9 0.1496013405408121 1105 10-11 0.25400895240576915 1105 12-13 0.16084121007867225 1105 14-15 0.2590525284519245 1105 16-17 0.22854089993940363 1105 18-19 0.17129787300201116 1105 20-21 0.19058827334011852 1105 22-23 0.2300235763778673 1105 24-25 0.17706857366282946 1105 26-27 0.27344052589930357 1105 28-29 0.29754466307297633 1105 30-31 0.3180267312705283 1105 32-33 0.3035704712097882 1105 34-35 0.28410483561246025 1105 36-37 0.23244567579523334 1105 38-39 0.15249129703202158 1105 40-41 0.18779866369204967 1105 42-43 0.2185105054342955 1105 44-45 0.17412950733298516 1105 46-47 0.133561907780539 1105 48-49 0.03664282663272189 1105 50-51 0.0943287216918236 1105 52-53 0.03760203798950812 1105 54-55 0.1433311878171324 1105 56-57 0.09248222271833839 1105 58-59 -0.008186276458680197 1105 60-61 0.08152913164249753 1105 62-63 0.05145057111958096 1105 64-65 0.136812738304549 1105 66-67 0.16201679085654774 1105 68-69 0.09953724948572784 1105 70-71 0.15045078990934257 1105 72-73 0.11782935593220856 1105 74-75 0.1395648442075128 1105 76-77 0.0747234750997805 1105 78-79 0.07128321098783985 1105 80-81 0.06359916886859551 1105 82-83 0.0531799853189483 1105 84-85 -0.008998653447410021 1105 86-87 0.009595948712764368 1105 88-89 0.050032888694637734 1105 90-91 0.037869580013413895 1105 92-93 0.0648932962801787 1105 94-95 0.04523132833615051 1105 96-97 0.0282802920207601 1105 98-99 0.0029608939140288726 1105 100 0.010809695742000969 1106 1 0.097637131520802 1106 2 0.060232173279047174 1106 3 0.10067814175381073 1106 4 0.12579923530518755 1106 5 0.07685663800675258 1106 6 0.14147381972081519 1106 7 0.20379107691269382 1106 8 0.18538407506155608 1106 9 0.2673622870189547 1106 10-11 0.30564694074983834 1106 12-13 0.2225655547370451 1106 14-15 0.25726971536540333 1106 16-17 0.17344991945422805 1106 18-19 0.23446400017905944 1106 20-21 0.2576317436567592 1106 22-23 0.24960982710171464 1106 24-25 0.28116689542388684 1106 26-27 0.27797090591172235 1106 28-29 0.4182899819114638 1106 30-31 0.3571062646587606 1106 32-33 0.31288233179668623 1106 34-35 0.29515128018366 1106 36-37 0.29554053529628277 1106 38-39 0.33624980055332543 1106 40-41 0.3225805681473588 1106 42-43 0.30899792172551344 1106 44-45 0.2157781852859486 1106 46-47 0.20968264849709328 1106 48-49 0.2079730684570933 1106 50-51 0.20875502449514727 1106 52-53 0.24091835569050346 1106 54-55 0.2974967789585534 1106 56-57 0.24950549214801754 1106 58-59 0.27710696498220244 1106 60-61 0.33181354229317606 1106 62-63 0.2878084532648799 1106 64-65 0.3447846472305045 1106 66-67 0.31601650065008613 1106 68-69 0.27402463489007545 1106 70-71 0.2825359334615385 1106 72-73 0.2663309932803912 1106 74-75 0.29963140253863685 1106 76-77 0.36199945345162376 1106 78-79 0.3113223823500171 1106 80-81 0.29981728246077566 1106 82-83 0.21764066333802745 1106 84-85 0.29142859816900213 1106 86-87 0.3300192266273001 1106 88-89 0.3455714869677742 1106 90-91 0.35633810546982403 1106 92-93 0.3187080428553273 1106 94-95 0.3845606403714221 1106 96-97 0.3818511859619065 1106 98-99 0.4367375805483036 1106 100 0.397778601009378 1107 1 0.024965039400104416 1107 2 0.008998339453146542 1107 3 0.12777338823243412 1107 4 0.03774619537834667 1107 5 0.019385151694478964 1107 6 0.05791292222015443 1107 7 0.13630087600748908 1107 8 0.1358657675702375 1107 9 0.17109924579094127 1107 10-11 0.14625751773151308 1107 12-13 0.08239462710159273 1107 14-15 0.14988811955907266 1107 16-17 0.14377370728826122 1107 18-19 0.21354985037357466 1107 20-21 0.2339097718235621 1107 22-23 0.2118297788847201 1107 24-25 0.246943462222589 1107 26-27 0.29267485460658804 1107 28-29 0.3346796156599865 1107 30-31 0.27078604666423445 1107 32-33 0.26685005006910245 1107 34-35 0.2500078708377984 1107 36-37 0.31989896226456693 1107 38-39 0.3292739241224254 1107 40-41 0.29322180537893416 1107 42-43 0.3258114233567113 1107 44-45 0.28205724591288117 1107 46-47 0.2571547645555299 1107 48-49 0.2495922293837225 1107 50-51 0.2415582314911262 1107 52-53 0.22407514291302988 1107 54-55 0.3103748242785045 1107 56-57 0.32433466272905775 1107 58-59 0.3229923880044723 1107 60-61 0.2773924472280811 1107 62-63 0.26142990575709035 1107 64-65 0.37644739992186516 1107 66-67 0.2959638861580203 1107 68-69 0.3268045298471449 1107 70-71 0.33535047237136695 1107 72-73 0.35696331945422344 1107 74-75 0.39324314382090364 1107 76-77 0.40965140152065516 1107 78-79 0.36453111492632573 1107 80-81 0.3922012033687352 1107 82-83 0.4066197326048453 1107 84-85 0.3916455343805687 1107 86-87 0.36715091414777845 1107 88-89 0.36992020659498337 1107 90-91 0.3285601181283724 1107 92-93 0.32809270276979774 1107 94-95 0.405835711580135 1107 96-97 0.3713402282818876 1107 98-99 0.3939568975731973 1107 100 0.40340265105944084 1108 1 0.1031535551507261 1108 2 0.17076088616588336 1108 3 0.14425985157029686 1108 4 0.16218014730154096 1108 5 0.07123420481241283 1108 6 0.13855266727905757 1108 7 0.20713234133195613 1108 8 0.2263508822890543 1108 9 0.2942690849804279 1108 10-11 0.3362248106643264 1108 12-13 0.24083257319841067 1108 14-15 0.297779110953833 1108 16-17 0.250895795682176 1108 18-19 0.30198620329338155 1108 20-21 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-0.8244499350007999 2215 44-45 -0.5363697556516982 2215 46-47 -0.410568670629722 2215 48-49 -0.409420686254073 2215 50-51 -0.334583698764753 2215 52-53 -0.33512201556201404 2215 54-55 -0.42984194229391903 2215 56-57 -0.45244160627484575 2215 58-59 -0.43475905368882195 2215 60-61 -0.4525863842352038 2215 62-63 -0.44729271575699414 2215 64-65 -0.4158983478021341 2215 66-67 -0.411999976265065 2215 68-69 -0.5427151019168974 2215 70-71 -0.4206831173693857 2215 72-73 -0.46070009063685546 2215 74-75 -0.316550120552229 2215 76-77 -0.3131904737090885 2215 78-79 -0.3032459266885006 2215 80-81 -0.39766104491553733 2215 82-83 -0.4348164706864637 2215 84-85 -0.5004908781348938 2215 86-87 -0.4228614231348722 2215 88-89 -0.4539982724327274 2215 90-91 -0.4355254139615141 2215 92-93 -0.3887679960605439 2215 94-95 -0.4385792578322345 2215 96-97 -0.4285942809291896 2215 98-99 -0.44222845689211354 2215 100 -0.5290318171063966 2216 1 -0.628506748370004 2216 2 -0.6064340719400256 2216 3 -0.7104786266817626 2216 4 -0.5457058303669626 2216 5 -0.8290770867400141 2216 6 -0.8523295911418671 2216 7 -0.8338956550395125 2216 8 -0.862013832983969 2216 9 -1.0641832355150314 2216 10-11 -1.2208327094498657 2216 12-13 -1.26892580967381 2216 14-15 -1.540900574418508 2216 16-17 -1.4088502162865097 2216 18-19 -1.3815494520669134 2216 20-21 -1.3442086743385744 2216 22-23 -1.1639287238160563 2216 24-25 -1.30307679857993 2216 26-27 -1.3717808637128641 2216 28-29 -4.754917491215345 2216 30-31 -2.24903967701389 2216 32-33 -1.7336978776052732 2216 34-35 -1.5550844045999739 2216 36-37 -1.592921703127189 2216 38-39 -1.584852289722889 2216 40-41 -1.5255515136491766 2216 42-43 -1.668070610424735 2216 44-45 -1.4997541709843532 2216 46-47 -1.4191106451397886 2216 48-49 -1.5026346801970973 2216 50-51 -1.462527419011053 2216 52-53 -1.5608718227412268 2216 54-55 -1.772366250121685 2216 56-57 -1.6073307844473703 2216 58-59 -1.6589807707136757 2216 60-61 -1.5367492201115738 2216 62-63 -1.5906999530868902 2216 64-65 -1.6283600196685342 2216 66-67 -1.782167430489352 2216 68-69 -1.7069882102455907 2216 70-71 -1.7432368738636512 2216 72-73 -1.7875237778954656 2216 74-75 -1.7178721815344922 2216 76-77 -1.6107186181715605 2216 78-79 -1.6139606035291258 2216 80-81 -1.534566998182143 2216 82-83 -1.624307382438829 2216 84-85 -1.6093189053673562 2216 86-87 -1.5879442079472312 2216 88-89 -1.5440583494387639 2216 90-91 -1.7167409375232339 2216 92-93 -1.597281045204177 2216 94-95 -1.5445128602245255 2216 96-97 -1.662880802643535 2216 98-99 -1.918003619847937 2216 100 -1.9581535199787758 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 149761.0 3 29234.0 4 19817.0 5 17398.0 6 12708.0 7 12223.0 8 13462.0 9 13924.0 10 14286.0 11 14943.0 12 16149.0 13 17756.0 14 19032.0 15 19697.0 16 19498.0 17 19312.0 18 18788.0 19 17451.0 20 16882.0 21 17937.0 22 22278.0 23 32042.0 24 48286.0 25 59852.0 26 63238.0 27 55208.0 28 59021.0 29 72076.0 30 82664.0 31 96969.0 32 120934.0 33 158208.0 34 216051.0 35 326460.0 36 518333.0 37 904534.0 38 1203880.0 39 521333.0 40 1551.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.817685584323485 21.452800522931398 13.490511555736775 22.239002337008344 2 15.97392378294726 22.417463783692885 38.96280129357835 22.6458111397815 3 17.745206623124616 28.038297437748323 30.017976712571897 24.198519226555167 4 12.220399782576406 19.733174211943584 38.23436732490648 29.81205868057353 5 12.134891978971888 39.53206124718225 33.94076012234206 14.392286651503802 6 28.027454390590467 39.98748647756242 18.525347815438433 13.45971131640868 7 25.158771158332893 34.96975291687778 22.37669393085163 17.494781993937693 8 23.843404302574267 36.96493710333258 20.959368762759542 18.232289831333613 9 24.40265755221863 19.21577461571227 21.50331678444431 34.87825104762479 10-11 22.38793480218003 29.261890257686694 28.620340647425234 19.729834292708038 12-13 23.210468432967353 27.497106798211174 28.040866128241614 21.251558640579862 14-15 21.27111352247036 29.452869329116627 25.692665918272766 23.583351230140245 16-17 20.32800964359814 32.25989013135417 26.172120935225962 21.239979289821733 18-19 20.215112848034696 30.902084281863885 28.242735115275213 20.6400677548262 20-21 22.11478771960874 30.196577675522157 27.356485605802593 20.33214899906651 22-23 21.61335093437623 30.42576042315477 27.353093458642608 20.607795183826394 24-25 21.203874396515577 30.289407794302548 27.56980282085621 20.936914988325668 26-27 20.991558378463058 29.981419310828954 27.77109066860736 21.255931642100627 28-29 21.015623969664134 30.104741867802275 27.814350793087783 21.065283369445805 30-31 21.47704048169821 30.72002991924204 27.146625801360464 20.656303797699284 32-33 20.746856905636328 30.34226892928413 27.825691481751498 21.085182683328043 34-35 21.02460850644199 29.68120126965846 27.92659665579402 21.367593568105534 36-37 21.532083727745324 30.257754328930826 27.268211423136286 20.941950520187564 38-39 21.24629888023745 30.285205961372025 27.565480930167258 20.903014228223267 40-41 20.965855702460477 29.89241422580411 27.849395978291486 21.29233409344393 42-43 20.888370363224322 29.911645619546018 27.827232598188694 21.372751419040974 44-45 21.273594095545832 30.05833365143276 27.655118087596154 21.012954165425253 46-47 21.099789763075954 30.42061067461027 27.37041041214131 21.109189150172462 48-49 20.852135380090605 29.93625919008749 27.82356895987439 21.38803646994751 50-51 20.96003864378326 29.73390062967673 27.79122164662612 21.514839079913887 52-53 20.975483863803586 29.84374161899439 27.68808129167699 21.492693225525034 54-55 21.145633982736648 30.395273627253328 27.408462568538926 21.050629821471098 56-57 20.81452642990219 30.077531784720236 27.470973047247305 21.63696873813027 58-59 20.78355978531636 29.528914901373643 27.873249682211064 21.81427563109893 60-61 21.257103787401004 30.142277512982606 27.277314192068147 21.32330450754824 62-63 21.093638846732066 30.05522397989216 27.438859165119396 21.41227800825638 64-65 21.01261733851166 29.711419416624206 27.764698440384443 21.511264804479687 66-67 20.892695515592496 29.802448672357635 27.43618727553746 21.86866853651241 68-69 21.512807819403296 30.057107945905543 27.282872761981547 21.14721147270961 70-71 21.480566448698056 29.75591759390576 27.43615415488779 21.327361802508385 72-73 21.106335658354972 29.835046768954925 27.855147956027675 21.203469616662424 74-75 21.154666973095683 29.85514586097251 27.60896035197828 21.381226813953532 76-77 21.232901091472748 29.875143972936215 27.254219347682263 21.637735587908782 78-79 21.46112360642296 30.010930988037384 27.412558292406157 21.115387113133497 80-81 21.177649630367142 29.798152956081296 27.53365720287838 21.490540210673178 82-83 21.055680561401005 29.801882672306057 27.737536305812053 21.404900460480885 84-85 21.174871298021078 29.663560475157276 27.519657762392015 21.641910464429632 86-87 21.534389525237135 29.810921139162783 27.316173164497826 21.338516171102256 88-89 21.15861353202641 29.63398616086017 27.506896568134444 21.700503738978973 90-91 21.086591792709694 29.49828209221676 27.469144187062877 21.94598192801067 92-93 21.32252242069659 29.780723083886194 27.27661470904774 21.620139786369467 94-95 21.20322604184265 29.529777810561953 27.524972814254134 21.742023333341265 96-97 21.20112601164024 29.55811290633289 27.44806552385983 21.79269555816704 98-99 21.104152402073257 29.60789247684104 27.42777678962523 21.86017833146047 100 21.340881956296588 29.74044023851491 27.19282956169881 21.72584824348969 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 30923.0 1 23148.5 2 14255.0 3 11247.0 4 7689.0 5 5342.0 6 4452.0 7 4401.5 8 4810.5 9 4955.5 10 3975.5 11 2896.0 12 2633.5 13 2669.5 14 2774.5 15 2892.0 16 3136.0 17 3626.5 18 4464.0 19 5549.5 20 6918.0 21 9146.5 22 12814.0 23 18168.5 24 24758.5 25 32697.0 26 43433.5 27 56748.5 28 72457.5 29 88144.5 30 100422.5 31 112037.5 32 123557.5 33 133762.0 34 143256.0 35 151039.5 36 156184.5 37 160987.0 38 164340.0 39 165001.5 40 165667.5 41 164836.5 42 161471.5 43 158958.5 44 156192.0 45 152618.0 46 150795.5 47 149353.0 48 148941.5 49 149576.0 50 149195.0 51 144771.5 52 137551.0 53 129768.0 54 123850.5 55 119948.5 56 111159.5 57 99838.0 58 91222.0 59 83945.0 60 74002.5 61 62573.0 62 53210.5 63 44225.0 64 35487.0 65 27693.0 66 21507.5 67 16606.5 68 12505.5 69 9427.5 70 7035.0 71 5345.0 72 4185.5 73 3216.5 74 2486.5 75 1882.5 76 1342.5 77 1013.0 78 783.5 79 635.0 80 485.5 81 293.5 82 208.0 83 175.0 84 157.0 85 142.5 86 112.5 87 80.0 88 70.0 89 61.5 90 47.0 91 38.0 92 29.0 93 25.0 94 19.5 95 18.5 96 24.5 97 23.5 98 19.5 99 22.0 100 30.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01649754043880285 2 0.0083280853176649 3 0.010727367040135027 4 0.009616955664446373 5 0.011758463317560204 6 0.01251195675106322 7 0.010727367040135027 8 0.007554763109596016 9 0.006741783352395395 10-11 0.006107262566287594 12-13 0.006345207861078018 14-15 0.007475448011332541 16-17 0.004858049768637859 18-19 0.005710687074970217 20-21 0.005125738225277087 22-23 0.005809830947799561 24-25 0.005442998618330988 26-27 0.006067605017155856 28-29 0.016408310953256438 30-31 0.0053041971963699065 32-33 0.005066251901579481 34-35 0.004858049768637859 36-37 0.00657323876858551 38-39 0.005482656167462726 40-41 0.005343854745501644 42-43 0.004867964155920793 44-45 0.004035155624154302 46-47 0.00582965972236543 48-49 0.0034601211617441077 50-51 0.0037972103293638765 52-53 0.002984230572163256 54-55 0.004739077121242645 56-57 0.0029247442484656495 58-59 0.0025281687571482732 60-61 0.0035592650345734516 62-63 0.0033213197397830253 64-65 0.0046498476356962365 66-67 0.0031230319941243376 68-69 0.0036187513582710577 70-71 0.004917536092335465 72-73 0.002785942826504568 74-75 0.0027264565028069614 76-77 0.0025281687571482732 78-79 0.003440292387178239 80-81 0.003945926138607893 82-83 0.0015565588034207015 84-85 0.0025777406935629456 86-87 0.0024091961097530605 88-89 0.0017449321617964552 90-91 0.0024389392716018636 92-93 0.0024488536588847983 94-95 0.0018242472600599306 96-97 0.0013186135086302759 98-99 0.001883733583757537 100 0.0027165421155240267 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5043176.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.22789922554246 #Duplication Level Percentage of deduplicated Percentage of total 1 75.57794800966762 47.030569324188846 2 15.107595161988069 18.802278185609726 3 4.723409249654352 8.817835043654584 4 1.8105356625821925 4.506633230216617 5 0.881492271431952 2.7426706117381014 6 0.48249153694845126 1.8014660843043193 7 0.3147609388905946 1.3710838389794735 8 0.2071150926993879 1.0310669693269119 9 0.13970445594066094 0.7824163325071217 >10 0.6943149722370014 7.474780577763754 >50 0.03763301212294995 1.6008902632498145 >100 0.021498027589603307 2.408526273377108 >500 0.001118219929092977 0.473671137995734 >1k 3.514392914637613E-4 0.40812143806618995 >5k 0.0 0.0 >10k+ 3.194902649626326E-5 0.7479906890218023 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 37623 0.7460179854916822 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.9828774565868808E-5 0.0 5 0.0 0.0 0.0 3.9657549131737616E-5 0.0 6 0.0 0.0 0.0 3.9657549131737616E-5 0.0 7 0.0 0.0 0.0 5.948632369760643E-5 0.0 8 0.0 0.0 0.0 5.948632369760643E-5 0.0 9 0.0 0.0 0.0 5.948632369760643E-5 0.0 10-11 0.0 0.0 0.0 7.931509826347523E-5 0.0 12-13 0.0 0.0 0.0 7.931509826347523E-5 0.0 14-15 0.0 0.0 0.0 1.5863019652695047E-4 0.0 16-17 0.0 0.0 0.0 2.181165202245569E-4 0.0 18-19 0.0 0.0 0.0 2.676884566392289E-4 0.0 20-21 0.0 0.0 0.0 3.370891676197698E-4 0.0 22-23 0.0 0.0 0.0 5.552056878443267E-4 0.0 24-25 0.0 0.0 0.0 0.0014673293178742918 0.0 26-27 0.0 0.0 0.0 0.002825600375636305 0.0 28-29 0.0 0.0 0.0 0.006593067543151379 0.0 30-31 0.0 0.0 0.0 0.015139269381040836 0.0 32-33 0.0 0.0 0.0 0.03290585139205929 0.0 34-35 0.0 0.0 0.0 0.060913995466348986 0.0 36-37 0.0 0.0 0.0 0.10350620323383519 0.0 38-39 0.0 0.0 0.0 0.16184245800662123 0.0 40-41 0.0 0.0 0.0 0.22843739738609162 0.0 42-43 0.0 0.0 0.0 0.296846669638339 0.0 44-45 0.0 0.0 0.0 0.37900719705201646 0.0 46-47 0.0 0.0 0.0 0.4786963611819219 0.0 48-49 0.0 0.0 0.0 0.5823215370631523 0.0 50-51 0.0 0.0 0.0 0.6962576757186345 0.0 52-53 0.0 0.0 0.0 0.8053754221546106 1.9828774565868808E-5 54-55 0.0 0.0 0.0 0.9348077481333191 1.9828774565868808E-5 56-57 0.0 0.0 0.0 1.0865970174350448 1.9828774565868808E-5 58-59 0.0 0.0 0.0 1.253297525210304 1.9828774565868808E-5 60-61 0.0 0.0 0.0 1.4263234120720751 1.9828774565868808E-5 62-63 0.0 0.0 0.0 1.604831955101309 1.9828774565868808E-5 64-65 0.0 0.0 0.0 1.8037244783842563 1.9828774565868808E-5 66-67 0.0 0.0 0.0 2.0383087958857673 1.9828774565868808E-5 68-69 0.0 0.0 0.0 2.2950914265137685 1.9828774565868808E-5 70-71 0.0 0.0 0.0 2.5732098185746444 1.9828774565868808E-5 72-73 0.0 0.0 0.0 2.8764215248486273 1.9828774565868808E-5 74-75 0.0 0.0 0.0 3.183420527064691 1.9828774565868808E-5 76-77 0.0 0.0 0.0 3.504914363488405 1.9828774565868808E-5 78-79 0.0 0.0 0.0 3.874314519263258 1.9828774565868808E-5 80-81 0.0 0.0 0.0 4.279366811707543 1.9828774565868808E-5 82-83 0.0 0.0 0.0 4.701769281896963 1.9828774565868808E-5 84-85 0.0 0.0 0.0 5.124102351375403 1.9828774565868808E-5 86-87 0.0 0.0 0.0 5.5765850725812465 1.9828774565868808E-5 88 0.0 0.0 0.0 5.940542229737768 1.9828774565868808E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6535 0.0 44.240723 1 GTATCAA 11225 0.0 35.430454 1 TACCTGG 4280 0.0 32.288895 2 ATCAACG 12595 0.0 30.19256 3 TCAACGC 12565 0.0 30.18983 4 TATCAAC 14040 0.0 27.95561 2 GTACCTG 4970 0.0 27.903479 1 CAACGCA 13950 0.0 27.25961 5 AACGCAG 14795 0.0 25.795975 6 GTACATG 20415 0.0 25.422157 1 TACATGG 20305 0.0 24.515606 2 TCTGTCG 1250 0.0 24.064243 8 CATGGGT 7890 0.0 23.770895 4 ACGCAGA 15950 0.0 23.57414 7 GTCTCGC 1400 0.0 23.505133 1 CGCAGAG 15840 0.0 23.470566 8 ACATGGG 21195 0.0 22.465994 3 ACCTGGG 6085 0.0 22.247515 3 GTGGTAT 3610 0.0 21.616907 1 CTGTCGC 1560 0.0 21.391243 9 >>END_MODULE