##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431434_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5043176
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27244656938405	33.0	31.0	34.0	30.0	34.0
2	32.393639246379664	34.0	31.0	34.0	31.0	34.0
3	32.49293520590993	34.0	31.0	34.0	31.0	34.0
4	36.04629542970541	37.0	35.0	37.0	35.0	37.0
5	35.96106203709726	37.0	35.0	37.0	35.0	37.0
6	35.99925602437829	37.0	35.0	37.0	35.0	37.0
7	35.942215381735636	37.0	35.0	37.0	35.0	37.0
8	35.924946303678475	37.0	35.0	37.0	35.0	37.0
9	37.61622318951391	39.0	37.0	39.0	35.0	39.0
10-11	37.548333431155285	39.0	37.0	39.0	34.5	39.0
12-13	37.530822442048425	39.0	37.0	39.0	35.0	39.0
14-15	38.870735425454114	40.0	38.0	41.0	35.0	41.0
16-17	38.92034860571989	40.0	38.0	41.0	35.0	41.0
18-19	38.99727165976361	40.5	38.5	41.0	35.0	41.0
20-21	39.00750380315896	41.0	39.0	41.0	35.0	41.0
22-23	38.93100875321424	40.0	38.5	41.0	35.0	41.0
24-25	38.863409486403015	40.5	38.0	41.0	35.0	41.0
26-27	38.71008844426608	40.0	38.0	41.0	34.5	41.0
28-29	38.603542589035165	40.0	38.0	41.0	34.0	41.0
30-31	38.40077661378464	40.0	38.0	41.0	34.0	41.0
32-33	38.16253121049117	40.0	37.5	41.0	34.0	41.0
34-35	37.987649548617775	40.0	37.0	41.0	33.0	41.0
36-37	37.81822268348358	40.0	37.0	41.0	33.0	41.0
38-39	37.69474652480897	40.0	37.0	41.0	33.0	41.0
40-41	37.55902589955218	40.0	37.0	41.0	33.0	41.0
42-43	37.42183298778389	40.0	37.0	41.0	33.0	41.0
44-45	37.19024192294697	40.0	37.0	41.0	32.0	41.0
46-47	36.85002238668649	40.0	36.0	41.0	31.0	41.0
48-49	36.57469350663153	40.0	36.0	41.0	30.0	41.0
50-51	36.43766061307399	40.0	36.0	41.0	30.0	41.0
52-53	36.297400983031324	40.0	35.5	41.0	30.0	41.0
54-55	36.139127506158815	40.0	35.0	41.0	29.5	41.0
56-57	35.923317667279505	39.0	35.0	41.0	29.0	41.0
58-59	35.758341668028244	39.0	35.0	41.0	28.5	41.0
60-61	35.54826026297714	39.0	35.0	41.0	28.0	41.0
62-63	35.31415788384145	38.5	35.0	41.0	28.0	41.0
64-65	35.064738272072994	38.0	35.0	41.0	28.0	41.0
66-67	34.80292399075503	37.0	35.0	40.0	28.0	41.0
68-69	34.50997625305958	37.0	34.5	40.0	27.0	41.0
70-71	34.17543081978499	36.5	34.0	39.5	26.0	41.0
72-73	33.85444529399727	36.0	34.0	39.0	26.0	41.0
74-75	33.55023659693812	35.5	34.0	39.0	26.0	41.0
76-77	33.202001575991005	35.0	34.0	37.5	26.0	40.0
78-79	32.89381364045197	35.0	34.0	37.0	25.5	39.0
80-81	32.58892273440388	35.0	33.5	37.0	25.0	39.0
82-83	32.33411564458587	35.0	33.0	36.0	25.0	39.0
84-85	32.10513097302176	35.0	33.0	36.0	25.0	37.0
86-87	31.862617624290728	35.0	33.0	36.0	24.5	37.0
88-89	31.712596090241547	35.0	33.0	35.0	24.0	37.0
90-91	31.52821495819301	35.0	33.0	35.0	24.0	36.0
92-93	31.330877308267645	35.0	33.0	35.0	23.0	36.0
94-95	31.17158354179985	35.0	33.0	35.0	21.0	36.0
96-97	31.003982411083808	35.0	33.0	35.0	20.0	36.0
98-99	30.8392743977208	35.0	33.0	35.0	18.5	35.5
100	30.72975065712559	35.0	33.0	35.0	15.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17048430673134618
1101	2	0.24760962254902097
1101	3	0.19331081366146208
1101	4	0.15197440627984093
1101	5	0.15822944042656673
1101	6	0.19704966981117877
1101	7	0.20310490966316053
1101	8	0.26107817873212014
1101	9	0.32887496005362493
1101	10-11	0.33802241220686824
1101	12-13	0.34218700212332465
1101	14-15	0.46260903029822487
1101	16-17	0.4155302243929526
1101	18-19	0.388229167862022
1101	20-21	0.3693796620401173
1101	22-23	0.35116118034632393
1101	24-25	0.3703958302991808
1101	26-27	0.35991739827196767
1101	28-29	0.39420722987837564
1101	30-31	0.44762708227487735
1101	32-33	0.3894835081027779
1101	34-35	0.44749436132889286
1101	36-37	0.44269670934756533
1101	38-39	0.37250676113832526
1101	40-41	0.45816384014482736
1101	42-43	0.45227517353154667
1101	44-45	0.46707489291151205
1101	46-47	0.5417770733271396
1101	48-49	0.5126524768654974
1101	50-51	0.48072093360267587
1101	52-53	0.5054019507845382
1101	54-55	0.4523510861397426
1101	56-57	0.49513260519340463
1101	58-59	0.453679645340479
1101	60-61	0.4964194701727891
1101	62-63	0.4934593845228079
1101	64-65	0.43597572377306903
1101	66-67	0.4150242661826411
1101	68-69	0.42376337644208917
1101	70-71	0.48275757087389337
1101	72-73	0.4775910901540854
1101	74-75	0.45061914018243954
1101	76-77	0.41679189889332235
1101	78-79	0.49641739258866835
1101	80-81	0.4958193610205157
1101	82-83	0.4381453261744639
1101	84-85	0.39793934723392965
1101	86-87	0.40971526809713055
1101	88-89	0.3586910222900421
1101	90-91	0.39509779056172434
1101	92-93	0.4406898176596208
1101	94-95	0.4170929258052034
1101	96-97	0.43897849561303914
1101	98-99	0.4702923185081005
1101	100	0.48026416678193584
1102	1	0.1707883206434957
1102	2	0.2370028293504518
1102	3	0.22150060300268848
1102	4	0.16713954304454148
1102	5	0.158781251221086
1102	6	0.19289102871849195
1102	7	0.1947707400685701
1102	8	0.2109437586037899
1102	9	0.3199890202105067
1102	10-11	0.3242442122100684
1102	12-13	0.378408933901035
1102	14-15	0.44728582495036306
1102	16-17	0.4393209348582161
1102	18-19	0.3907162798089203
1102	20-21	0.35537618846424834
1102	22-23	0.3475619625597659
1102	24-25	0.3580516713864341
1102	26-27	0.39112280654079257
1102	28-29	0.31225422431485583
1102	30-31	0.3115697571229248
1102	32-33	0.3107344170934354
1102	34-35	0.3432319731573017
1102	36-37	0.3606587903223897
1102	38-39	0.3697334856560204
1102	40-41	0.34977805549153373
1102	42-43	0.32611444796896905
1102	44-45	0.3661622632580688
1102	46-47	0.3071985013613343
1102	48-49	0.2808835704174868
1102	50-51	0.29125806877230076
1102	52-53	0.2174941669863628
1102	54-55	0.20407500071824103
1102	56-57	0.22976937080650828
1102	58-59	0.23881230881332982
1102	60-61	0.2516807901600018
1102	62-63	0.2277417172720746
1102	64-65	0.19660750344448275
1102	66-67	0.17275750350304975
1102	68-69	0.1718660364827187
1102	70-71	0.21609270850425588
1102	72-73	0.15923361471837438
1102	74-75	0.19777400705066128
1102	76-77	0.1472116067634417
1102	78-79	0.16195198451039516
1102	80-81	0.17170039013477378
1102	82-83	0.13665296980512665
1102	84-85	0.20977589049276446
1102	86-87	0.20321093950747837
1102	88-89	0.20810136180400463
1102	90-91	0.19585822066558833
1102	92-93	0.23722241492086127
1102	94-95	0.2527581508588206
1102	96-97	0.25780228046460607
1102	98-99	0.32619500526855205
1102	100	0.3454623789465856
1103	1	0.1478844032859783
1103	2	0.18884456988594422
1103	3	0.1739298512429741
1103	4	0.17116497928397933
1103	5	0.15265851017028353
1103	6	0.13099746231391407
1103	7	0.14268278913306176
1103	8	0.17470258121534243
1103	9	0.23978155364702047
1103	10-11	0.25668091479412425
1103	12-13	0.23444121278921415
1103	14-15	0.3335477861034022
1103	16-17	0.3738237232744126
1103	18-19	0.3643594350191677
1103	20-21	0.31580884057778746
1103	22-23	0.3180487213990446
1103	24-25	0.352372072420124
1103	26-27	0.40339996064150085
1103	28-29	0.33108701936208007
1103	30-31	0.3117723357987785
1103	32-33	0.356108583748572
1103	34-35	0.31824850739836563
1103	36-37	0.3116122376441979
1103	38-39	0.3312605944251956
1103	40-41	0.35922866094079353
1103	42-43	0.40039031832949235
1103	44-45	0.4024628453413541
1103	46-47	0.4220945476755631
1103	48-49	0.3219832580740629
1103	50-51	0.31495091041596623
1103	52-53	0.3419329196626748
1103	54-55	0.35652911384139685
1103	56-57	0.38026489183826584
1103	58-59	0.35987818070190514
1103	60-61	0.37154135339608985
1103	62-63	0.3867399981206958
1103	64-65	0.3644345214639202
1103	66-67	0.3299728950388783
1103	68-69	0.2998140880225222
1103	70-71	0.2846828838264983
1103	72-73	0.3705538214262276
1103	74-75	0.37876889505606925
1103	76-77	0.33153109939377856
1103	78-79	0.39649218843001677
1103	80-81	0.3362879909646921
1103	82-83	0.2720152630059829
1103	84-85	0.27290177932270154
1103	86-87	0.25535535050855174
1103	88-89	0.2591727755045632
1103	90-91	0.2788443142550072
1103	92-93	0.2958065331702393
1103	94-95	0.34908718692428664
1103	96-97	0.2602528127610064
1103	98-99	0.27162912418170393
1103	100	0.29575778533674324
1104	1	0.08828934377908837
1104	2	0.13555881668929715
1104	3	0.10369042269284279
1104	4	0.061171907130287195
1104	5	0.032564181914871426
1104	6	0.09034597928831545
1104	7	0.10546759492393676
1104	8	0.10320389273068287
1104	9	0.16059403519791005
1104	10-11	0.1822314836666905
1104	12-13	0.17111081669285255
1104	14-15	0.2606490246213653
1104	16-17	0.22230073373727777
1104	18-19	0.22363800031304493
1104	20-21	0.21589678985937866
1104	22-23	0.20324420410606336
1104	24-25	0.2173066179466474
1104	26-27	0.22517248461919337
1104	28-29	0.25154838844686367
1104	30-31	0.24867055710485886
1104	32-33	0.19798655646614094
1104	34-35	0.26487965094156607
1104	36-37	0.2818370117949911
1104	38-39	0.3110873185494114
1104	40-41	0.3189197368960137
1104	42-43	0.31755894839451315
1104	44-45	0.3022508409624294
1104	46-47	0.3426720706113926
1104	48-49	0.32813220322240255
1104	50-51	0.31326328964924954
1104	52-53	0.36556803991857834
1104	54-55	0.33978291533639293
1104	56-57	0.3803659793495626
1104	58-59	0.3841777360770564
1104	60-61	0.334327923283702
1104	62-63	0.37818983123332117
1104	64-65	0.37262466958053153
1104	66-67	0.3352104696663716
1104	68-69	0.334505012360772
1104	70-71	0.3369841852187747
1104	72-73	0.3269397047922311
1104	74-75	0.3055617503917816
1104	76-77	0.3220976254272827
1104	78-79	0.34235759955819844
1104	80-81	0.33165829546838665
1104	82-83	0.33246814077795506
1104	84-85	0.3103977891265828
1104	86-87	0.2361395405247677
1104	88-89	0.2343275039532422
1104	90-91	0.28862395168106403
1104	92-93	0.3401029642823765
1104	94-95	0.34362051811145733
1104	96-97	0.40429599395876537
1104	98-99	0.3943108483644693
1104	100	0.43974137820716663
1105	1	0.028098838492077505
1105	2	0.09551260265457273
1105	3	0.08089370072593027
1105	4	0.040559615095574486
1105	5	0.055625642829788546
1105	6	0.07451943126513072
1105	7	0.07339290710702073
1105	8	0.08997350634859913
1105	9	0.12606293172616034
1105	10-11	0.07370692846203752
1105	12-13	-0.0062246992231962395
1105	14-15	0.11037786736633137
1105	16-17	0.11116414256296991
1105	18-19	0.123178326834541
1105	20-21	0.11969266893928676
1105	22-23	0.10674836807310584
1105	24-25	0.12424687438758042
1105	26-27	0.1393921725007985
1105	28-29	0.1020500691240187
1105	30-31	0.1153733198755873
1105	32-33	0.08101201184623363
1105	34-35	0.1517442195477514
1105	36-37	0.13378233893942593
1105	38-39	0.14456837364568997
1105	40-41	0.13702397027640245
1105	42-43	0.15858421094738873
1105	44-45	0.1880489011917632
1105	46-47	0.1404828515064125
1105	48-49	0.10955570069079101
1105	50-51	0.14114661909847825
1105	52-53	0.15213974060016966
1105	54-55	0.12163816118015802
1105	56-57	0.12783674380347065
1105	58-59	0.107325859890814
1105	60-61	0.14224533221963753
1105	62-63	0.15512899305914374
1105	64-65	0.17499148470537307
1105	66-67	0.18774559344851127
1105	68-69	0.18273364302172723
1105	70-71	0.12503198531485538
1105	72-73	0.12670660515101417
1105	74-75	0.07375191457997232
1105	76-77	0.15041241597566568
1105	78-79	0.09098650423990762
1105	80-81	0.07535108093171772
1105	82-83	0.11298460524601239
1105	84-85	0.08262676985679462
1105	86-87	0.050943961568826523
1105	88-89	0.020125773616211262
1105	90-91	-0.007072170338165762
1105	92-93	0.012759806818561259
1105	94-95	0.06289422152354973
1105	96-97	0.03316270887053818
1105	98-99	0.09170156283306596
1105	100	0.18925470446967552
1106	1	0.05196193641494773
1106	2	0.14706842792178065
1106	3	0.09575954841289303
1106	4	0.09209648869456544
1106	5	0.01395486665300183
1106	6	0.03013610715626669
1106	7	0.00996465738146668
1106	8	0.06840900345971335
1106	9	0.11128488017393323
1106	10-11	0.0983374875713352
1106	12-13	0.07761593482440077
1106	14-15	0.10900969435669339
1106	16-17	0.13223342461772347
1106	18-19	0.13948865150446466
1106	20-21	0.10979221257660754
1106	22-23	0.07739186328732472
1106	24-25	0.13138707814188422
1106	26-27	0.1336820420999416
1106	28-29	0.13213096887689346
1106	30-31	0.09883497842649547
1106	32-33	0.09892410511187677
1106	34-35	0.13899129298882684
1106	36-37	0.16455745204272176
1106	38-39	0.16553329493967084
1106	40-41	0.1632409992110766
1106	42-43	0.1549640625608859
1106	44-45	0.17895759787722199
1106	46-47	0.13258056645241112
1106	48-49	0.11803117424831555
1106	50-51	0.09206108410753444
1106	52-53	0.07068442174395528
1106	54-55	0.0749505728121278
1106	56-57	0.09248520066569199
1106	58-59	0.07838130071765192
1106	60-61	0.09004378491719933
1106	62-63	0.013922409784875356
1106	64-65	0.05202821488290965
1106	66-67	0.12750990413709928
1106	68-69	0.10599705446450969
1106	70-71	0.14244321564666507
1106	72-73	0.1599466648111516
1106	74-75	0.1580037166431083
1106	76-77	0.13731936303154413
1106	78-79	0.12667576863705676
1106	80-81	0.08654060336450442
1106	82-83	0.06561842780364202
1106	84-85	0.11422296217731542
1106	86-87	0.09300484734737324
1106	88-89	0.04775628415281474
1106	90-91	0.06607042783645056
1106	92-93	0.1175667184340945
1106	94-95	0.13730167175706498
1106	96-97	0.07449388308805283
1106	98-99	0.16756873456892052
1106	100	0.2377476471975548
1107	1	0.054886378428804505
1107	2	0.12250954084311161
1107	3	0.057573295453408946
1107	4	0.09192912498981087
1107	5	0.02254257496801415
1107	6	0.04800762103597833
1107	7	0.008565446162720036
1107	8	-0.03225935928342949
1107	9	0.00334179733941653
1107	10-11	0.026441965150986846
1107	12-13	0.009969018393825024
1107	14-15	0.1386984187929059
1107	16-17	0.12422278431675693
1107	18-19	0.024459605363801984
1107	20-21	0.028653736318588585
1107	22-23	0.02310803389391225
1107	24-25	0.09097514466130008
1107	26-27	0.09422716100352346
1107	28-29	0.08553936316994992
1107	30-31	0.09758941401750576
1107	32-33	0.07616381951135054
1107	34-35	0.097909676074309
1107	36-37	0.1055621769678936
1107	38-39	0.04976039741037397
1107	40-41	0.07427750037866332
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2216	74-75	-0.7510468839957269
2216	76-77	-0.6332997911987022
2216	78-79	-0.5782785766223668
2216	80-81	-0.6326893986348097
2216	82-83	-0.7117351353754664
2216	84-85	-0.6989403182557652
2216	86-87	-0.7075136204937422
2216	88-89	-0.6915749242718476
2216	90-91	-0.7133235512939784
2216	92-93	-0.6992176397310956
2216	94-95	-0.7967893957529881
2216	96-97	-0.9684124246413326
2216	98-99	-1.0555789840622474
2216	100	-1.0967517413196148
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	239.0
3	811.0
4	521.0
5	457.0
6	847.0
7	1821.0
8	3483.0
9	5376.0
10	7283.0
11	9790.0
12	13226.0
13	18826.0
14	19886.0
15	24503.0
16	30367.0
17	42868.0
18	48109.0
19	27819.0
20	18546.0
21	16869.0
22	17002.0
23	17814.0
24	20091.0
25	24712.0
26	30553.0
27	39083.0
28	49002.0
29	61844.0
30	77892.0
31	97486.0
32	121445.0
33	154209.0
34	209643.0
35	319238.0
36	483581.0
37	918078.0
38	1394462.0
39	710815.0
40	4579.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.64258444700671	20.968139987573096	14.268324327927445	24.12095123749275
2	14.284093991564045	21.844032411321752	39.534947818596855	24.336925778517347
3	16.052476496934865	27.500727716603755	31.51205233531595	24.93474345114543
4	11.074534772787521	18.612818888788354	38.54691004681242	31.765736291611702
5	11.308424447041682	38.02958417724807	35.05508464661471	15.606906729095535
6	27.800635948457874	39.31062489193317	18.384446626490924	14.504292533118019
7	24.398890699035686	34.154489155246615	23.30089213622527	18.14572800949243
8	24.765207480365547	34.360886869702746	21.498059952696476	19.375845697235235
9	24.98185667127223	15.845431529655121	22.899736197983174	36.27297560108948
10-11	22.71900404911932	27.02040503885116	29.54754492642454	20.713045985604982
12-13	24.370942041285097	25.240651922518666	29.609040017639675	20.779366018556562
14-15	21.833533075189127	26.710935727803275	28.511675975615365	22.943855221392234
16-17	19.52164667661807	29.77650393323572	28.920822910007505	21.781026480138706
18-19	19.251658082129197	27.18285064808367	33.24995994587538	20.315531323911756
20-21	20.882753249143	26.5272221314505	32.95106099806947	19.63896362133703
22-23	20.41459588164284	26.792402248107145	32.95901233667038	19.833989533579633
24-25	20.09976909250081	26.621522879076448	33.14604279670721	20.132665231715528
26-27	19.916050917120483	26.392198091044218	33.304826165099136	20.38692482673617
28-29	19.962699977524014	26.529623635813298	33.28264091535457	20.22503547130812
30-31	20.502625791041424	27.065174962345996	32.670002210013116	19.762197036599463
32-33	19.73301919238717	26.715946503108214	33.25973546606535	20.291298838439264
34-35	19.966500627245715	26.262723004862405	33.12151831038951	20.649258057502365
36-37	20.62105146579921	26.87587566874545	32.291148511535404	20.211924353919937
38-39	20.432803321925665	26.893205943409004	32.44256160745605	20.23142912720928
40-41	20.12242022350842	26.64560299068771	32.614763754429056	20.617213031374817
42-43	20.14469175544121	26.7273248780925	32.41147670994953	20.716506656516756
44-45	20.548311222927772	26.918751199640862	31.997356427774875	20.535581149656487
46-47	20.53972573512528	27.40280189738406	31.27794630106477	20.77952606642589
48-49	20.523412686806516	27.068356548805532	31.26697041186044	21.14126035252751
50-51	20.64627530078978	26.989389558819845	30.972506287553177	21.3918288528372
52-53	20.67585216679445	27.30614988173514	30.714628684430966	21.303369267039443
54-55	20.83751725122543	27.91144172655023	30.37924935357477	20.871791668649568
56-57	20.48736542630792	27.406660771874108	30.552340344355567	21.55363345746241
58-59	20.328951902087827	26.85903782361868	31.045718226360673	21.766292047932822
60-61	20.779901660141938	27.444313771446783	30.41831420728783	21.357470361123454
62-63	20.60706462720163	27.283127715554134	30.672442663469134	21.437364993775102
64-65	20.490755673991927	27.014821478090795	30.995204258950388	21.49921858896689
66-67	20.450343553620616	27.153406625829934	30.571969387520394	21.824280433029053
68-69	21.0497772566735	27.280383943603354	30.337082884827726	21.332755914895422
70-71	21.07968175521627	27.018594683256257	30.368368099220287	21.53335546230718
72-73	20.71740109803822	27.096912739115194	30.681350403000014	21.504335759846573
74-75	20.693861959093343	27.10980377499088	30.429306837006802	21.76702742890897
76-77	20.771741854735986	27.183118336540307	30.035090982349217	22.01004882637449
78-79	20.97139364991614	27.28049118546241	30.147064947157986	21.601050217463463
80-81	20.670278015282435	27.087622958231083	30.28606378202942	21.956035244457066
82-83	20.50267186266572	27.05229966185555	30.48022341764835	21.964805057830375
84-85	20.614880991271605	26.973996450821247	30.248078651815728	22.163043906091414
86-87	20.74869090430316	27.117395863241732	30.034426718401264	22.099486514053844
88-89	20.318733671004143	27.028027576273367	30.182141174529704	22.47109757819279
90-91	20.263728650358427	26.831861509493226	30.140728778848885	22.76368106129947
92-93	20.418542430220207	27.107868793200147	29.965189543042236	22.508399233537407
94-95	20.245952153960122	26.775042155974727	30.26408358542315	22.714922104641996
96-97	20.143850369917097	26.859425554270867	30.17363925017603	22.823084825636005
98-99	20.07555188898064	26.786884364980544	30.100642325892522	23.036921420146292
100	20.275220243811372	26.89412453816168	29.84975097959395	22.980904238433
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7252.0
1	4319.5
2	7400.0
3	12506.0
4	7478.5
5	3417.0
6	6022.5
7	14076.5
8	22207.0
9	23175.0
10	15404.0
11	7636.0
12	5931.5
13	6106.5
14	6698.0
15	7257.0
16	7774.5
17	8404.5
18	9092.0
19	10108.5
20	11485.5
21	13262.0
22	16173.0
23	21236.5
24	27521.0
25	34615.5
26	44739.5
27	57135.5
28	71906.5
29	87570.5
30	99544.5
31	110279.0
32	121420.5
33	131399.0
34	140631.0
35	147422.5
36	152632.5
37	158151.0
38	161366.5
39	162340.0
40	163225.5
41	163168.5
42	160680.5
43	158593.5
44	155855.5
45	152299.5
46	150473.5
47	149043.0
48	148239.0
49	148660.0
50	149212.0
51	144919.0
52	136567.0
53	128430.0
54	121744.5
55	116491.5
56	107731.0
57	97341.0
58	89947.0
59	82905.5
60	72272.5
61	60655.5
62	51032.5
63	41986.0
64	33284.5
65	25346.5
66	19427.0
67	14689.5
68	10769.5
69	7908.5
70	5655.5
71	4097.5
72	3114.0
73	2362.5
74	1800.0
75	1274.0
76	830.5
77	587.5
78	418.5
79	293.0
80	189.5
81	84.5
82	43.5
83	18.0
84	13.0
85	10.0
86	3.5
87	1.5
88	1.0
89	1.0
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	1.0
96	2.5
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04957193641467202
2	0.0
3	7.931509826347523E-5
4	0.0691627656857504
5	0.00416404265883245
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.011857607190389549
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.146920115419331E-4
26-27	0.0
28-29	3.0734600577096656E-4
30-31	0.004352416017208204
32-33	8.922948554640965E-5
34-35	0.00191347674560634
36-37	0.004848135381354924
38-39	0.0015268156415718984
40-41	0.0010905826011227845
42-43	0.0021910795895285036
44-45	0.0
46-47	4.362330404491138E-4
48-49	4.65976202297917E-4
50-51	0.0015367300288548326
52-53	9.220380173128997E-4
54-55	0.0011104113756886533
56-57	0.006087433791721724
58-59	0.009785500248256258
60-61	0.001050925051991047
62-63	0.0024091961097530605
64-65	0.001100496988405719
66-67	7.931509826347523E-5
68-69	7.83236595351818E-4
70-71	2.7760284392216333E-4
72-73	0.0
74-75	2.2803090750749132E-4
76-77	0.0
78-79	5.948632369760644E-4
80-81	0.0
82-83	0.0012888703467814728
84-85	7.435790462200804E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	1.487158092440161E-4
94-95	0.0
96-97	3.767467167515074E-4
98-99	3.8666110403444176E-4
100	2.974316184880322E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5043176.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.80399863930656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.12017645338517	40.9671440512464
2	17.1100306882843	19.438363198715898
3	5.324843145716838	9.074171484114606
4	2.1330120098195215	4.846544452136505
5	1.034079344140405	2.9369920828743297
6	0.5873148200704865	2.0017098144077092
7	0.3473451561693709	1.381141564489027
8	0.25931512850045996	1.1784108965193387
9	0.17571599303950902	0.8983233926568603
>10	0.8299322942810685	8.270583835543706
>50	0.0477374837234772	1.8622484700057613
>100	0.02790900044697951	2.9505650770169134
>500	0.0016440381139257282	0.61326032146212
>1k	6.646089577924143E-4	0.5585971177815897
>5k	6.995883766106104E-5	0.3067485613132594
>10k+	2.098765129831831E-4	2.715195679716084
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38911	0.7715574471325213	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32592	0.6462594206507962	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24782	0.4913966912913608	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19068	0.37809507342198645	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10888	0.21589569747317958	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10409	0.20639771445612842	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8068	0.15997855319742957	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7370	0.14613806855045314	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.9828774565868808E-5	0.0
5	0.0	0.0	0.0	1.9828774565868808E-5	0.0
6	0.0	0.0	0.0	1.9828774565868808E-5	0.0
7	0.0	0.0	0.0	3.9657549131737616E-5	0.0
8	0.0	0.0	0.0	3.9657549131737616E-5	0.0
9	0.0	0.0	0.0	7.931509826347523E-5	0.0
10-11	0.0	0.0	0.0	1.0905826011227846E-4	0.0
12-13	0.0	0.0	0.0	1.3880142196108166E-4	0.0
14-15	0.0	0.0	0.0	1.883733583757537E-4	0.0
16-17	0.0	0.0	0.0	2.181165202245569E-4	0.0
18-19	0.0	0.0	0.0	2.676884566392289E-4	0.0
20-21	0.0	0.0	0.0	3.1726039305390093E-4	0.0
22-23	0.0	0.0	0.0	5.254625259955235E-4	0.0
24-25	0.0	0.0	0.0	0.001447500543308423	0.0
26-27	0.0	0.0	0.0	0.0027363708900898956	0.0
28-29	0.0	0.0	0.0	0.006384865410209757	0.0
30-31	0.0	0.0	0.0	0.014990553571796818	0.0
32-33	0.0	0.0	0.0	0.032985166490322765	0.0
34-35	0.0	0.0	0.0	0.061260999021251684	0.0
36-37	0.0	0.0	0.0	0.10435884054016754	0.0
38-39	0.0	0.0	0.0	0.16328995854992967	0.0
40-41	0.0	0.0	0.0	0.23097548053052283	0.0
42-43	0.0	0.0	0.0	0.3004158490601954	0.0
44-45	0.0	0.0	0.0	0.3840932777281618	0.0
46-47	0.0	0.0	0.0	0.4854282301470343	0.0
48-49	0.0	0.0	0.0	0.5908082525773442	0.0
50-51	0.0	0.0	0.0	0.7063306931980958	0.0
52-53	0.0	0.0	0.0	0.8170545703739072	0.0
54-55	0.0	0.0	0.0	0.9487176334912761	0.0
56-57	0.0	3.9657549131737616E-5	0.0	1.10337216071777	0.0
58-59	0.0	3.9657549131737616E-5	0.0	1.2728090393831188	0.0
60-61	0.0	3.9657549131737616E-5	0.0	1.4497907667707808	0.0
62-63	0.0	3.9657549131737616E-5	0.0	1.6317990885108908	0.0
64-65	0.0	3.9657549131737616E-5	0.0	1.8345879659960311	0.0
66-67	0.0	3.9657549131737616E-5	0.0	2.0724936032373247	0.0
68-69	0.0	3.9657549131737616E-5	0.0	2.3323695226976016	0.0
70-71	0.0	3.9657549131737616E-5	0.0	2.6142553819259926	0.0
72-73	0.0	3.9657549131737616E-5	0.0	2.9215815589223935	0.0
74-75	0.0	3.9657549131737616E-5	0.0	3.232199312496728	0.0
76-77	0.0	3.9657549131737616E-5	0.0	3.558699914498324	0.0
78-79	0.0	3.9657549131737616E-5	0.0	3.9335529832787914	0.0
80-81	0.0	3.9657549131737616E-5	0.0	4.345664319468526	0.0
82-83	0.0	3.9657549131737616E-5	0.0	4.774402083131741	0.0
84-85	0.0	3.9657549131737616E-5	0.0	5.203625651771819	0.0
86-87	0.0	3.9657549131737616E-5	0.0	5.6654675545727535	0.0
88	0.0	3.9657549131737616E-5	0.0	6.036910074127891	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13470	0.0	61.54902	1
GTATCAA	25290	0.0	42.81053	1
GTGGTAT	4615	0.0	36.602062	1
TGGTATC	4595	0.0	35.925423	2
ATCAACG	33365	0.0	32.37801	3
TATAACG	495	0.0	32.30383	2
TCAACGC	33705	0.0	32.135117	4
TATCAAC	33665	0.0	31.977715	2
CAACGCA	35090	0.0	30.840187	5
TACCTGG	4385	0.0	30.138176	2
AACGCAG	36585	0.0	29.681854	6
ATAACGC	545	0.0	29.340176	3
GTACCTG	5185	0.0	26.407486	1
ACGCAGA	41095	0.0	26.275688	7
CGCAGAG	41170	0.0	26.18215	8
GCAGAGT	43405	0.0	24.498245	9
GTACATG	19600	0.0	23.622284	1
TCTGTCG	1370	0.0	23.332993	8
TACATGG	19525	0.0	23.244303	2
ACCTGGG	5565	0.0	23.071602	3
>>END_MODULE