##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431433_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4859399
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.03747171203682	31.0	31.0	34.0	26.0	34.0
2	30.035764916608	31.0	31.0	34.0	25.0	34.0
3	30.146733989120875	31.0	31.0	34.0	26.0	34.0
4	33.461718208362804	37.0	35.0	37.0	27.0	37.0
5	33.61997749104365	37.0	35.0	37.0	28.0	37.0
6	33.715327553880634	37.0	35.0	37.0	28.0	37.0
7	33.611116312943224	37.0	35.0	37.0	28.0	37.0
8	33.539013569373495	37.0	35.0	37.0	28.0	37.0
9	34.88917168563437	39.0	35.0	39.0	27.0	39.0
10-11	34.83369507216839	39.0	35.0	39.0	27.0	39.0
12-13	34.79870648613131	39.0	35.0	39.0	27.0	39.0
14-15	35.984382842405	40.0	36.0	41.0	27.0	41.0
16-17	35.996105691259345	40.0	36.0	41.0	27.0	41.0
18-19	35.98616423553612	40.0	36.0	41.0	27.0	41.0
20-21	36.08496307876756	40.0	36.0	41.0	27.0	41.0
22-23	36.05352215366551	40.0	36.0	41.0	27.0	41.0
24-25	36.08203102894	40.0	36.0	41.0	27.0	41.0
26-27	36.06691527079789	40.0	36.0	41.0	27.0	41.0
28-29	35.9538725879476	40.0	36.0	41.0	27.0	41.0
30-31	35.93601328065466	40.0	36.0	41.0	27.0	41.0
32-33	35.882425995478044	40.0	36.0	41.0	27.0	41.0
34-35	35.78174502649402	40.0	35.5	41.0	27.0	41.0
36-37	35.662940005543895	40.0	35.0	41.0	26.5	41.0
38-39	35.565025839615146	40.0	35.0	41.0	26.0	41.0
40-41	35.48407231017663	40.0	35.0	41.0	26.0	41.0
42-43	35.27870668780234	40.0	35.0	41.0	25.0	41.0
44-45	35.157729793334525	39.5	35.0	41.0	25.0	41.0
46-47	35.031694660183284	39.0	35.0	41.0	25.0	41.0
48-49	34.90359651471303	39.0	34.5	41.0	24.0	41.0
50-51	34.7452443193078	39.0	34.0	41.0	24.0	41.0
52-53	34.660982561835326	39.0	34.0	41.0	24.0	41.0
54-55	34.59504323477039	39.0	34.0	41.0	24.0	41.0
56-57	34.44272645650213	39.0	34.0	41.0	23.5	41.0
58-59	34.2396269579839	38.0	34.0	40.5	23.0	41.0
60-61	34.03088550662335	38.0	33.0	40.0	22.5	41.0
62-63	33.766222736597676	38.0	33.0	40.0	21.5	41.0
64-65	33.63176310486132	37.0	33.0	40.0	21.5	41.0
66-67	33.38575202406717	37.0	33.0	40.0	20.0	41.0
68-69	33.129459733600804	36.5	33.0	40.0	20.0	41.0
70-71	32.840426768824706	36.0	32.5	39.0	18.5	41.0
72-73	32.53256945972126	35.5	32.0	39.0	18.0	41.0
74-75	32.200647960786924	35.0	32.0	39.0	14.5	40.5
76-77	31.86892298821315	35.0	32.0	37.5	12.5	40.0
78-79	31.523987225580775	35.0	32.0	37.0	8.0	39.0
80-81	31.211070957540223	35.0	31.0	37.0	7.0	39.0
82-83	30.891311662203492	35.0	31.0	36.0	2.0	38.5
84-85	30.587053460726317	35.0	31.0	36.0	2.0	37.0
86-87	30.31338011140884	35.0	31.0	35.5	2.0	37.0
88-89	30.029907710809503	35.0	31.0	35.0	2.0	36.5
90-91	29.79196017861468	35.0	31.0	35.0	2.0	36.0
92-93	29.583476372283897	35.0	30.0	35.0	2.0	36.0
94-95	29.34788252621363	34.0	30.0	35.0	2.0	36.0
96-97	29.234296051836864	34.0	30.0	35.0	2.0	35.5
98-99	29.064739590225045	34.0	30.0	35.0	2.0	35.0
100	28.92453634698447	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.29226991155900706
1101	2	0.34010784117253934
1101	3	0.3860663437616232
1101	4	0.42768530996775667
1101	5	0.2744396098217692
1101	6	0.33730532906277233
1101	7	0.4099889342981484
1101	8	0.43706104604953566
1101	9	0.5345227843819416
1101	10-11	0.6554030114672074
1101	12-13	0.5429448383498681
1101	14-15	0.7222682336894479
1101	16-17	0.6682853865365246
1101	18-19	0.6115539912799477
1101	20-21	0.5841822609211107
1101	22-23	0.5811449737331529
1101	24-25	0.5551955145723397
1101	26-27	0.6360027183823078
1101	28-29	0.7677703261690283
1101	30-31	0.7166266579531921
1101	32-33	0.6702519916740215
1101	34-35	0.6828494117652468
1101	36-37	0.6845266073991212
1101	38-39	0.6897325116726094
1101	40-41	0.6622827584271462
1101	42-43	0.7247958393819047
1101	44-45	0.7075830425796426
1101	46-47	0.6485958754298409
1101	48-49	0.6566868367188619
1101	50-51	0.6370564672288097
1101	52-53	0.6418975956934361
1101	54-55	0.6563570021772307
1101	56-57	0.5840838990930166
1101	58-59	0.656091532398456
1101	60-61	0.6407341218434723
1101	62-63	0.6461568477551936
1101	64-65	0.6148182940594396
1101	66-67	0.6403142549483647
1101	68-69	0.6457220645455024
1101	70-71	0.6586218306472063
1101	72-73	0.6604156205326461
1101	74-75	0.58751277986552
1101	76-77	0.6189713522561462
1101	78-79	0.6005604321636504
1101	80-81	0.6471465002101056
1101	82-83	0.6319062476618491
1101	84-85	0.6122973941865801
1101	86-87	0.6610466196346181
1101	88-89	0.6456867502101993
1101	90-91	0.660625880547002
1101	92-93	0.5900843276375731
1101	94-95	0.6006072168660488
1101	96-97	0.6243641605508259
1101	98-99	0.614555565373422
1101	100	0.6349380465231746
1102	1	0.22933281376774417
1102	2	0.2894439478298061
1102	3	0.2220489312746352
1102	4	0.26585794226247117
1102	5	0.28317104107853197
1102	6	0.34422991663541325
1102	7	0.39056868004415435
1102	8	0.3251341955834093
1102	9	0.5020566042930028
1102	10-11	0.517452885906657
1102	12-13	0.48919435384145515
1102	14-15	0.5617296472391331
1102	16-17	0.47840092093318276
1102	18-19	0.48831196135991206
1102	20-21	0.5552188930947821
1102	22-23	0.507813651860701
1102	24-25	0.5801295747016795
1102	26-27	0.5882957615312563
1102	28-29	0.5967893206788943
1102	30-31	0.5917155605188285
1102	32-33	0.6090018954398602
1102	34-35	0.622670685456093
1102	36-37	0.5231404260463393
1102	38-39	0.48050384461700446
1102	40-41	0.4952945250282639
1102	42-43	0.560638269903059
1102	44-45	0.43551727225910497
1102	46-47	0.40207345542879835
1102	48-49	0.32581382709722817
1102	50-51	0.2983205591365561
1102	52-53	0.3271666263606505
1102	54-55	0.35522644546093574
1102	56-57	0.3913215981419711
1102	58-59	0.3952970854545228
1102	60-61	0.37816573996138914
1102	62-63	0.38120094636483515
1102	64-65	0.42873350920343256
1102	66-67	0.4650781864287481
1102	68-69	0.4480015792850125
1102	70-71	0.4030158624825759
1102	72-73	0.41319900177168023
1102	74-75	0.4759674616964631
1102	76-77	0.42155863831035845
1102	78-79	0.4032534534179426
1102	80-81	0.3659366153929433
1102	82-83	0.31560444753786854
1102	84-85	0.3095791629193094
1102	86-87	0.32143988693685444
1102	88-89	0.38599865124988497
1102	90-91	0.3641084224461295
1102	92-93	0.3648286340240041
1102	94-95	0.3741106476973819
1102	96-97	0.333485490357873
1102	98-99	0.40015526329828077
1102	100	0.33328548900852084
1103	1	0.30379523933216035
1103	2	0.3174812271753389
1103	3	0.3606014474776167
1103	4	0.33402984432109406
1103	5	0.35112934803358087
1103	6	0.37528867404108723
1103	7	0.5053438098677177
1103	8	0.47591422871825984
1103	9	0.5114699447264783
1103	10-11	0.6437630504005725
1103	12-13	0.5400910294256818
1103	14-15	0.7037252766954225
1103	16-17	0.5894708885496982
1103	18-19	0.5802725203754164
1103	20-21	0.5696602596975779
1103	22-23	0.5472745766080536
1103	24-25	0.5977615422465234
1103	26-27	0.599421041674141
1103	28-29	0.6890421761808483
1103	30-31	0.5820821387804997
1103	32-33	0.6690367295517348
1103	34-35	0.5945942641335265
1103	36-37	0.6430317879546692
1103	38-39	0.6740904300738606
1103	40-41	0.6081324320325763
1103	42-43	0.6137473502123783
1103	44-45	0.526340691016344
1103	46-47	0.48426903841563274
1103	48-49	0.48197557703065996
1103	50-51	0.38351761571126275
1103	52-53	0.44458070891830204
1103	54-55	0.509245886596986
1103	56-57	0.4702529521692469
1103	58-59	0.4824393602154018
1103	60-61	0.48758262532864194
1103	62-63	0.3983633386248613
1103	64-65	0.3711674453167859
1103	66-67	0.39044622055362055
1103	68-69	0.4290647614359102
1103	70-71	0.41655850488905344
1103	72-73	0.46853156472411683
1103	74-75	0.3785923374471807
1103	76-77	0.3405281312011539
1103	78-79	0.34931741455519116
1103	80-81	0.3797120867374808
1103	82-83	0.4037985144233218
1103	84-85	0.42669494443785183
1103	86-87	0.28631058868860393
1103	88-89	0.42387944745742345
1103	90-91	0.43519972516947547
1103	92-93	0.4109840249113965
1103	94-95	0.41357182932399184
1103	96-97	0.43660093485082285
1103	98-99	0.42964073157440197
1103	100	0.44457675810636843
1104	1	0.27616765318826353
1104	2	0.3187025458262305
1104	3	0.304055307439981
1104	4	0.2804550133808448
1104	5	0.31355107572515095
1104	6	0.3386987575813549
1104	7	0.4177964218711736
1104	8	0.3276268535598774
1104	9	0.47644740166922617
1104	10-11	0.5078322417582086
1104	12-13	0.4195530953332849
1104	14-15	0.5593939393564611
1104	16-17	0.5907883312240969
1104	18-19	0.5173805413950561
1104	20-21	0.4792825865284769
1104	22-23	0.5171654468036806
1104	24-25	0.5631474384527948
1104	26-27	0.6694246483400974
1104	28-29	0.6940657362802796
1104	30-31	0.6417546656644788
1104	32-33	0.7194768240207097
1104	34-35	0.7028808597180145
1104	36-37	0.7085908004064976
1104	38-39	0.7633580681449885
1104	40-41	0.7161828717033671
1104	42-43	0.7042584830421603
1104	44-45	0.6345385718360745
1104	46-47	0.5917867244816009
1104	48-49	0.6243102896434962
1104	50-51	0.569018556108432
1104	52-53	0.534217208204133
1104	54-55	0.5931011283230703
1104	56-57	0.5830151327167812
1104	58-59	0.6154148976537925
1104	60-61	0.6243659017408874
1104	62-63	0.6319560208151742
1104	64-65	0.6572094641428308
1104	66-67	0.7161787084502294
1104	68-69	0.6820751267305525
1104	70-71	0.5927669947752747
1104	72-73	0.6317121079817127
1104	74-75	0.6957101928369269
1104	76-77	0.6229010788959073
1104	78-79	0.6614175483827331
1104	80-81	0.6327824184596089
1104	82-83	0.5550324908162452
1104	84-85	0.5383021486513009
1104	86-87	0.5919496628830174
1104	88-89	0.5417161642179096
1104	90-91	0.5004082497149014
1104	92-93	0.5442528945935301
1104	94-95	0.5119423359521562
1104	96-97	0.5930346504957065
1104	98-99	0.656073114379403
1104	100	0.6460864198934466
1105	1	0.2124633312403148
1105	2	0.24184603063762822
1105	3	0.32840913986147413
1105	4	0.29061480067210965
1105	5	0.2250221498770415
1105	6	0.3664778680384515
1105	7	0.3966067069472672
1105	8	0.36517499411893084
1105	9	0.46075370086541767
1105	10-11	0.4885130465309331
1105	12-13	0.41795530873809383
1105	14-15	0.49343125122803144
1105	16-17	0.4313912250434413
1105	18-19	0.4607096180056658
1105	20-21	0.4351402226799337
1105	22-23	0.4344903310348869
1105	24-25	0.47855653649903473
1105	26-27	0.47493363818438183
1105	28-29	0.5850433161800481
1105	30-31	0.5340894171871895
1105	32-33	0.5465992271344646
1105	34-35	0.5048817970856163
1105	36-37	0.4200502297291706
1105	38-39	0.4811876666952699
1105	40-41	0.4331404228983544
1105	42-43	0.4115101664436409
1105	44-45	0.3918086308222257
1105	46-47	0.42343424078065084
1105	48-49	0.38446184666415917
1105	50-51	0.44429693497476563
1105	52-53	0.42106295137367766
1105	54-55	0.500463360317859
1105	56-57	0.5076257636328592
1105	58-59	0.4990343647088409
1105	60-61	0.47373040039234837
1105	62-63	0.4280662222917968
1105	64-65	0.511072016455735
1105	66-67	0.5356453439569577
1105	68-69	0.5883353054173526
1105	70-71	0.5970380736324614
1105	72-73	0.532465523012057
1105	74-75	0.5229222256049653
1105	76-77	0.4530324388325013
1105	78-79	0.5561653349518174
1105	80-81	0.5380972702719795
1105	82-83	0.5133505563284757
1105	84-85	0.5100055448744776
1105	86-87	0.5024784814565812
1105	88-89	0.529011506996838
1105	90-91	0.5745838847334745
1105	92-93	0.49247562988502125
1105	94-95	0.46848366670501207
1105	96-97	0.5679095915338479
1105	98-99	0.6010889448030703
1105	100	0.5971157276461874
1106	1	0.17376161288414949
1106	2	0.2594759778471918
1106	3	0.2521977995086857
1106	4	0.2996759132022504
1106	5	0.2655427462800546
1106	6	0.3026611955294811
1106	7	0.41988266165073895
1106	8	0.37514008000307086
1106	9	0.5069483586407983
1106	10-11	0.5125278049605342
1106	12-13	0.3668560494340056
1106	14-15	0.47540938166262947
1106	16-17	0.4131317004324728
1106	18-19	0.4276052771961645
1106	20-21	0.43212310544154064
1106	22-23	0.4619406448107455
1106	24-25	0.4654680091771368
1106	26-27	0.43504605336444513
1106	28-29	0.5694643590034616
1106	30-31	0.5328636368260007
1106	32-33	0.5050418367735716
1106	34-35	0.47907005230810995
1106	36-37	0.4965897133961903
1106	38-39	0.5420573953569985
1106	40-41	0.5597331528266878
1106	42-43	0.5355710725359373
1106	44-45	0.4690906329358171
1106	46-47	0.49315775413694496
1106	48-49	0.438122096164129
1106	50-51	0.5025865871295068
1106	52-53	0.5240752646272355
1106	54-55	0.6066967715274743
1106	56-57	0.4367178155279703
1106	58-59	0.5005373077154687
1106	60-61	0.6066480610208203
1106	62-63	0.5978071619434928
1106	64-65	0.5610837816719396
1106	66-67	0.5399403637279789
1106	68-69	0.5395133438816586
1106	70-71	0.5481695233110244
1106	72-73	0.4983640989933136
1106	74-75	0.5686603016715281
1106	76-77	0.5290670881197457
1106	78-79	0.4879399470740573
1106	80-81	0.4049181184749955
1106	82-83	0.39583141806932787
1106	84-85	0.3896812509393257
1106	86-87	0.4554439969713613
1106	88-89	0.43381019642798435
1106	90-91	0.4212868323525498
1106	92-93	0.40808597451473005
1106	94-95	0.4497263115101333
1106	96-97	0.5330753518409672
1106	98-99	0.5080038350239846
1106	100	0.5092131172463397
1107	1	0.09840544623400405
1107	2	0.09115477360862911
1107	3	0.07036571375098433
1107	4	0.10566646864214846
1107	5	0.09896405045083867
1107	6	0.11111910060305519
1107	7	0.1831680163988878
1107	8	0.2167517976886444
1107	9	0.29722135368616165
1107	10-11	0.23141154722646462
1107	12-13	0.1443345437685224
1107	14-15	0.23269544884713866
1107	16-17	0.16930088963979983
1107	18-19	0.250792241896832
1107	20-21	0.19458089471140738
1107	22-23	0.3285499686576756
1107	24-25	0.2803012452890741
1107	26-27	0.323135305413075
1107	28-29	0.35160181612283026
1107	30-31	0.2885364628879046
1107	32-33	0.2891796716603494
1107	34-35	0.3170441190249136
1107	36-37	0.23568379678272322
1107	38-39	0.23887863060771508
1107	40-41	0.23547953859880266
1107	42-43	0.24820235186513884
1107	44-45	0.20189341792114135
1107	46-47	0.18460504181001625
1107	48-49	0.24050510397601244
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2216	3	-0.7190000673143615
2216	4	-0.48891750299526393
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2216	6	-0.7718712263545342
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2216	12-13	-1.2318684588138638
2216	14-15	-1.4371981914557495
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2216	70-71	-1.7365272810139736
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2216	80-81	-1.4298477006366817
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2216	84-85	-1.5178736346684936
2216	86-87	-1.5205217017765236
2216	88-89	-1.4506612781216717
2216	90-91	-1.6312142844145079
2216	92-93	-1.5290189359798845
2216	94-95	-1.447162065648122
2216	96-97	-1.5513872452711936
2216	98-99	-1.8569654483132823
2216	100	-1.834946893704661
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	147198.0
3	28242.0
4	19842.0
5	16628.0
6	12448.0
7	11606.0
8	12752.0
9	13201.0
10	13669.0
11	14219.0
12	15604.0
13	16752.0
14	17644.0
15	17321.0
16	17108.0
17	17048.0
18	16592.0
19	15936.0
20	16169.0
21	17642.0
22	21443.0
23	30885.0
24	44752.0
25	56404.0
26	62178.0
27	55162.0
28	58727.0
29	70954.0
30	83184.0
31	96221.0
32	120622.0
33	157106.0
34	215200.0
35	317626.0
36	501087.0
37	857274.0
38	1159390.0
39	492261.0
40	1302.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11535987832374	21.790402764471438	13.947100052585053	22.147137304619765
2	15.37707194003416	22.683402232501816	39.30656573025949	22.632960097204528
3	17.478129493648925	28.423086429738497	30.70216329031367	23.396620786298907
4	12.230151125248403	19.835744521112915	38.534098261870305	29.400006091768375
5	12.289325784885708	39.534925839548016	34.44198756761785	13.733760807948425
6	27.62424446489756	40.166127165345905	18.74626451590596	13.463363853850574
7	24.60774501517115	35.364824868545156	22.8877236028865	17.139706513397186
8	23.706755683244772	37.32600400903886	21.099954311774802	17.867285995941568
9	24.412743407852975	19.3217971231985	21.69438763781681	34.57107183113172
10-11	22.29709205408409	29.55556584552695	28.869142433784035	19.278199666604927
12-13	22.918225605032728	27.62980039826502	28.517736705497093	20.93423729120516
14-15	20.917408422204907	29.603740421559927	25.88682152148029	23.592029634754876
16-17	20.190228352225486	32.53580865828662	26.604752673301558	20.669210316186337
18-19	20.231274482323748	31.272969703555255	28.449514911965508	20.046240902155493
20-21	21.713012001925446	30.451117142817747	27.82141149755605	20.014459357700762
22-23	21.414230926231518	30.54265339909154	27.7056961715967	20.33741950308024
24-25	21.15733463013599	30.31888112492554	27.86556154439565	20.658222700542826
26-27	20.857685844400972	30.36932673828283	27.917771166812116	20.85521625050408
28-29	20.85500631459701	30.31937906188305	28.094769998830937	20.730844624689006
30-31	21.09265493028906	30.611114689707247	27.729419853772384	20.566810526231304
32-33	20.75717587145155	30.465739007564803	28.048556243959204	20.72852887702444
34-35	21.064468676311943	30.015356544723932	28.002943303439316	20.91723147552481
36-37	21.24360899516791	30.187800127822666	27.686141118819823	20.882449758189598
38-39	20.98650743899824	30.270715573718874	27.929912029374854	20.812864957908033
40-41	20.899523628470337	29.947866154983583	28.108276640199293	21.044333576346784
42-43	20.80452867651417	30.12948924589559	28.07743012795606	20.988551949634175
44-45	21.079012391665408	30.136379627606928	27.966297287811237	20.818310692916427
46-47	20.979268370234223	30.29342694743488	27.8410371309731	20.886267551357793
48-49	20.90951054169293	30.003021055397884	27.997404526520565	21.09006387638862
50-51	20.886677052442682	29.914481983696938	27.9824086631366	21.21643230072378
52-53	20.804729373276547	30.034417873463354	27.944691447659036	21.216161305601062
54-55	20.939584553318106	30.237347683904247	27.83161231232557	20.991455450452076
56-57	20.826512593840153	29.996683651761412	27.76171394803798	21.415089806360456
58-59	20.828537093646986	29.68439430939859	28.03550652767064	21.451562069283785
60-61	21.076777707247622	29.999869320846027	27.721112987151564	21.202239984754783
62-63	20.850782899884386	29.937920358772008	27.83170708517913	21.37958965616448
64-65	20.84354767676851	29.730417477846053	27.982612292609044	21.443422552776394
66-67	20.702184097364228	29.74982602726744	27.795754417075848	21.75223545829249
68-69	21.010553216307233	29.962569168630544	27.644866331174523	21.382011283887703
70-71	21.02076606125613	29.610624646736383	27.80381854497526	21.564790747032227
72-73	20.736367026338232	29.69334967056631	28.15399625621084	21.416287046884623
74-75	20.798230266242076	29.790439371246087	27.888391282462234	21.522939080049603
76-77	20.86812297043658	29.64771116128559	27.718005941432917	21.76615992684491
78-79	20.853850100334682	29.66604633608116	27.98465067663915	21.495452886945007
80-81	20.706835498055547	29.678690370671596	27.97876278989555	21.635711341377306
82-83	20.60797969751654	29.52840806312505	28.17324569120658	21.690366548151825
84-85	20.69830411856919	29.509221802234798	27.988603232273967	21.803870846922045
86-87	20.939187140307823	29.504731825818105	27.856142781749504	21.69993825212457
88-89	20.733015969868134	29.453312020282723	27.991601683984808	21.822070325864335
90-91	20.702663696816845	29.365170419777165	27.858507036175418	22.073658847230572
92-93	20.761563441497504	29.371604435224974	27.8841104031873	21.982721720090222
94-95	20.700838833862164	29.24103043120051	28.04600369332613	22.012127041611194
96-97	20.777186531661567	29.313263320143907	27.87249662890249	22.03705351929204
98-99	20.643188024637244	29.247712123737735	27.952662003453167	22.15643784817186
100	20.73611177993695	29.290311985647204	27.805662670586607	22.167913563829238
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24958.0
1	18849.0
2	11697.5
3	8981.5
4	6059.5
5	4285.0
6	3657.0
7	3701.0
8	4082.5
9	4284.5
10	3615.0
11	2752.0
12	2567.5
13	2620.0
14	2688.5
15	2814.0
16	3057.0
17	3573.0
18	4246.5
19	5287.0
20	7082.0
21	9403.0
22	13019.0
23	19259.5
24	26402.5
25	33620.5
26	44346.0
27	58872.0
28	77258.0
29	94789.0
30	104540.0
31	112902.0
32	122790.0
33	133448.0
34	143682.0
35	149794.5
36	153999.5
37	157947.0
38	160811.0
39	162503.5
40	164876.0
41	164740.0
42	160738.0
43	158703.0
44	157099.5
45	154330.5
46	153187.0
47	150522.5
48	146059.0
49	141366.0
50	137884.0
51	131855.5
52	122212.0
53	114900.0
54	110585.5
55	106526.0
56	98299.0
57	88129.5
58	79641.0
59	73359.5
60	65005.0
61	54896.0
62	47147.5
63	39041.0
64	30653.0
65	24014.5
66	18863.5
67	14866.0
68	11835.0
69	9218.0
70	7005.5
71	5417.0
72	4374.0
73	3499.5
74	2710.0
75	2033.0
76	1490.5
77	1163.0
78	892.0
79	615.5
80	418.5
81	299.5
82	224.0
83	162.0
84	123.0
85	103.5
86	88.0
87	71.0
88	50.5
89	46.0
90	41.5
91	31.5
92	22.5
93	23.5
94	21.0
95	14.0
96	14.5
97	15.5
98	21.5
99	27.0
100	37.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012429520605325886
2	0.007531795598591513
3	0.009425033836488832
4	0.00788163309907254
5	0.00977487133696986
6	0.012161997810840394
7	0.010412810308435261
8	0.007799318393077004
9	0.006626333832640621
10-11	0.006153024273166291
12-13	0.006646912509139505
14-15	0.0077067143488320266
16-17	0.004918303683233256
18-19	0.005782608096186381
20-21	0.005391613242707586
22-23	0.005854633463932474
24-25	0.005525374639950332
26-27	0.0061838922879146165
28-29	0.016771621346590392
30-31	0.005391613242707586
32-33	0.005154958462970421
34-35	0.0047948316242399525
36-37	0.006657201847388946
38-39	0.005700293390190844
40-41	0.005587110669446983
42-43	0.004753674271242184
44-45	0.004228918020520644
46-47	0.006214760302662943
48-49	0.003498375004810265
50-51	0.0038893698582890598
52-53	0.0030970908130820295
54-55	0.0045170194915050196
56-57	0.002850146695095422
58-59	0.0025414665476121636
60-61	0.003488085666560823
62-63	0.003230852210324775
64-65	0.004506730153255577
66-67	0.0033028775780708686
68-69	0.003488085666560823
70-71	0.005031486403977117
72-73	0.0031073801513314715
74-75	0.0028192786803470965
76-77	0.0027472533126010027
78-79	0.0033337455928191947
80-81	0.003951105887785712
82-83	0.0018932382378973203
84-85	0.0026032025771088154
86-87	0.00252088787111328
88-89	0.0018829488996478783
90-91	0.002438573165117744
92-93	0.002448862503367186
94-95	0.001924106252645646
96-97	0.0015742687521646194
98-99	0.0018315022084006685
100	0.0028192786803470965
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4859399.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.31064601765572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.07879600660114	41.714248593418446
2	15.147208797002577	17.058982254470656
3	4.92036860143131	8.312074037747589
4	2.048657419420016	4.614448910256183
5	1.0326882076258144	2.9075670053112295
6	0.630220544073755	2.1292875582234974
7	0.42062147111919895	1.657982673733317
8	0.2902641211965329	1.3075968144259107
9	0.21004826338835783	1.064515806565662
>10	1.1115340563281717	11.205857435199672
>50	0.07287304660767399	2.8118934474780795
>100	0.034302619168287085	3.4112069578870563
>500	0.0017943262537520005	0.6432100363674386
>1k	5.859009725616105E-4	0.49129900373725427
>5k	0.0	0.0
>10k+	3.661881078487315E-5	0.6698294651780432
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32484	0.6684777273897451	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.0578676498883915E-5	0.0	0.0	0.0	0.0
3	2.0578676498883915E-5	0.0	0.0	0.0	0.0
4	2.0578676498883915E-5	0.0	0.0	0.0	0.0
5	2.0578676498883915E-5	0.0	0.0	0.0	0.0
6	2.0578676498883915E-5	0.0	0.0	0.0	0.0
7	2.0578676498883915E-5	0.0	0.0	0.0	0.0
8	2.0578676498883915E-5	0.0	0.0	0.0	0.0
9	2.0578676498883915E-5	0.0	0.0	2.0578676498883915E-5	0.0
10-11	2.0578676498883915E-5	0.0	0.0	2.0578676498883915E-5	0.0
12-13	2.0578676498883915E-5	0.0	0.0	2.0578676498883915E-5	0.0
14-15	2.0578676498883915E-5	0.0	0.0	2.0578676498883915E-5	0.0
16-17	2.0578676498883915E-5	0.0	0.0	4.115735299776783E-5	0.0
18-19	2.0578676498883915E-5	2.0578676498883915E-5	0.0	7.20253677460937E-5	0.0
20-21	2.0578676498883915E-5	4.115735299776783E-5	0.0	1.9549742673939723E-4	0.0
22-23	2.0578676498883915E-5	4.115735299776783E-5	0.0	5.864922802181916E-4	0.0
24-25	2.0578676498883915E-5	4.115735299776783E-5	0.0	0.0012655886046813608	0.0
26-27	2.0578676498883915E-5	5.144669124720979E-5	0.0	0.002613491915358257	0.0
28-29	2.0578676498883915E-5	6.173602949665175E-5	0.0	0.005751740081438054	0.0
30-31	2.0578676498883915E-5	6.173602949665175E-5	0.0	0.013798002592501666	0.0
32-33	2.0578676498883915E-5	6.173602949665175E-5	0.0	0.031032644160316945	0.0
34-35	2.0578676498883915E-5	6.173602949665175E-5	0.0	0.0573836394171378	0.0
36-37	2.0578676498883915E-5	6.173602949665175E-5	0.0	0.09824260160567182	0.0
38-39	2.0578676498883915E-5	6.173602949665175E-5	0.0	0.15195294726775882	0.0
40-41	2.0578676498883915E-5	6.173602949665175E-5	0.0	0.21066391131907464	0.0
42-43	4.115735299776783E-5	6.173602949665175E-5	1.0289338249441957E-5	0.27120637757879107	0.0
44-45	4.115735299776783E-5	6.173602949665175E-5	2.0578676498883915E-5	0.3487365412883363	0.0
46-47	4.115735299776783E-5	6.173602949665175E-5	2.0578676498883915E-5	0.44315150906521567	0.0
48-49	4.115735299776783E-5	6.173602949665175E-5	2.0578676498883915E-5	0.5457876581033992	0.0
50-51	4.115735299776783E-5	8.231470599553566E-5	2.0578676498883915E-5	0.6468186703746698	0.0
52-53	4.115735299776783E-5	8.231470599553566E-5	2.0578676498883915E-5	0.7484876216174058	0.0
54-55	4.115735299776783E-5	8.231470599553566E-5	2.0578676498883915E-5	0.8658478136905408	0.0
56-57	4.115735299776783E-5	8.231470599553566E-5	2.0578676498883915E-5	1.0106599602131867	0.0
58-59	4.115735299776783E-5	8.231470599553566E-5	2.0578676498883915E-5	1.1725318295534077	0.0
60-61	4.115735299776783E-5	8.231470599553566E-5	2.0578676498883915E-5	1.3400937029455702	0.0
62-63	4.115735299776783E-5	8.231470599553566E-5	2.0578676498883915E-5	1.5089211649424137	0.0
64-65	4.115735299776783E-5	8.231470599553566E-5	2.0578676498883915E-5	1.6983273034381412	0.0
66-67	4.115735299776783E-5	8.231470599553566E-5	2.0578676498883915E-5	1.9190850555799184	0.0
68-69	4.115735299776783E-5	8.231470599553566E-5	2.0578676498883915E-5	2.165957148198779	0.0
70-71	4.115735299776783E-5	8.231470599553566E-5	2.0578676498883915E-5	2.4344677191562165	0.0
72-73	4.115735299776783E-5	8.231470599553566E-5	2.0578676498883915E-5	2.7221679059488633	0.0
74-75	4.115735299776783E-5	8.231470599553566E-5	2.0578676498883915E-5	3.013664858555554	0.0
76-77	4.115735299776783E-5	8.231470599553566E-5	2.0578676498883915E-5	3.336698632896784	0.0
78-79	6.173602949665175E-5	8.231470599553566E-5	2.0578676498883915E-5	3.699572724939854	0.0
80-81	6.173602949665175E-5	1.0289338249441958E-4	2.0578676498883915E-5	4.094755339086171	0.0
82-83	6.173602949665175E-5	1.0289338249441958E-4	2.0578676498883915E-5	4.50871599553772	0.0
84-85	6.173602949665175E-5	1.0289338249441958E-4	2.0578676498883915E-5	4.920711388383625	0.0
86-87	6.173602949665175E-5	1.0289338249441958E-4	2.0578676498883915E-5	5.360477705164774	0.0
88	6.173602949665175E-5	1.0289338249441958E-4	2.0578676498883915E-5	5.714492676975074	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6530	0.0	41.680267	1
TACCTGG	3835	0.0	35.66658	2
GTATCAA	10970	0.0	32.995087	1
ATCAACG	11870	0.0	29.81797	3
TCAACGC	11975	0.0	29.478016	4
GTACCTG	4780	0.0	29.109112	1
TATCAAC	13075	0.0	27.285625	2
CAACGCA	13250	0.0	27.067148	5
ACCTGGG	5030	0.0	26.071743	3
AACGCAG	14060	0.0	25.77366	6
GTACATG	19560	0.0	23.936224	1
CGCAGAG	15010	0.0	23.296003	8
ACGCAGA	15490	0.0	22.968788	7
TACATGG	20245	0.0	22.613876	2
GTGGTAT	3645	0.0	22.568653	1
ACATGGG	20560	0.0	21.535831	3
TATAACG	320	2.0008883E-11	20.564203	2
ATAACGC	395	0.0	20.229525	3
GCAGAGT	16390	0.0	19.929436	9
TGGTATC	4050	0.0	19.033676	2
>>END_MODULE