FastQCFastQC Report
Wed 25 May 2016
SRR2431433_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431433_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4859399
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346250.7125366737738555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301390.6202207309998623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211080.4343747035384417No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170330.35051659680548974No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90340.1859077634909173No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81610.16794257890739162No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74400.15310535315169632No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67960.13985268548641508No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127250.058.12041
GTATCAA231750.040.3986781
GTGGTAT44200.035.8708341
TATAACG4600.035.7776222
TACCTGG39250.032.8255652
ATAACGC5200.032.5537073
TGGTATC50900.030.9476952
ATCAACG303600.030.7129973
TCAACGC306500.030.3763774
TATCAAC309800.030.2653032
CAACGCA319250.029.1695485
AACGCAG333550.028.0866626
GTACCTG48750.026.249931
ACGCAGA375950.024.756487
CGCAGAG374100.024.7406888
ACCTGGG49650.024.3396973
GCAGAGT394800.023.0863049
GTACATG187500.022.2564681
TACATGG188900.021.3578052
AGAGTAC380150.021.1752910-11
Produced by FastQC (version 0.11.5)