##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431433_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4859399
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26566412842411	33.0	31.0	34.0	30.0	34.0
2	32.36752219770387	34.0	31.0	34.0	30.0	34.0
3	32.4809989877349	34.0	31.0	34.0	31.0	34.0
4	36.03600095402744	37.0	35.0	37.0	35.0	37.0
5	35.953277967090166	37.0	35.0	37.0	35.0	37.0
6	35.99284253052692	37.0	35.0	37.0	35.0	37.0
7	35.93120198608923	37.0	35.0	37.0	35.0	37.0
8	35.913464813241305	37.0	35.0	37.0	35.0	37.0
9	37.59159393990903	39.0	37.0	39.0	35.0	39.0
10-11	37.5320507741801	39.0	37.0	39.0	34.5	39.0
12-13	37.51377032427261	39.0	37.0	39.0	35.0	39.0
14-15	38.84244810932381	40.0	38.0	41.0	34.5	41.0
16-17	38.89254833776769	40.0	38.0	41.0	35.0	41.0
18-19	38.972771015510354	40.0	38.5	41.0	35.0	41.0
20-21	38.98834897072663	40.0	39.0	41.0	35.0	41.0
22-23	38.92092129088391	40.0	38.0	41.0	35.0	41.0
24-25	38.858852298401516	40.0	38.0	41.0	35.0	41.0
26-27	38.70550668920169	40.0	38.0	41.0	34.5	41.0
28-29	38.608733405098036	40.0	38.0	41.0	34.0	41.0
30-31	38.41756830422857	40.0	38.0	41.0	34.0	41.0
32-33	38.194440711701176	40.0	37.5	41.0	34.0	41.0
34-35	38.02502974133221	40.0	37.0	41.0	33.0	41.0
36-37	37.87121493830821	40.0	37.0	41.0	33.0	41.0
38-39	37.763310339406175	40.0	37.0	41.0	33.0	41.0
40-41	37.632128685049324	40.0	37.0	41.0	33.0	41.0
42-43	37.502160452352236	40.0	37.0	41.0	33.0	41.0
44-45	37.28945709129874	40.0	37.0	41.0	32.5	41.0
46-47	36.96833826569912	40.0	36.5	41.0	31.0	41.0
48-49	36.7177194134501	40.0	36.0	41.0	31.0	41.0
50-51	36.58781600358398	40.0	36.0	41.0	30.0	41.0
52-53	36.46242848138216	40.0	36.0	41.0	30.0	41.0
54-55	36.31468603421946	40.0	35.0	41.0	30.0	41.0
56-57	36.101998210066725	39.5	35.0	41.0	29.5	41.0
58-59	35.94337643399935	39.0	35.0	41.0	29.0	41.0
60-61	35.746217176239284	39.0	35.0	41.0	29.0	41.0
62-63	35.52575658018615	39.0	35.0	41.0	28.0	41.0
64-65	35.291625775121574	38.0	35.0	41.0	28.0	41.0
66-67	35.035632801504875	37.5	35.0	40.0	28.0	41.0
68-69	34.74819139156921	37.0	35.0	40.0	28.0	41.0
70-71	34.423799321685664	36.5	34.0	39.5	27.5	41.0
72-73	34.10004930650889	36.0	34.0	39.0	26.5	41.0
74-75	33.79062081545475	36.0	34.0	39.0	26.0	41.0
76-77	33.436174926158564	35.0	34.0	38.5	26.0	40.0
78-79	33.120808252213905	35.0	34.0	37.0	26.0	39.0
80-81	32.81163277598732	35.0	34.0	37.0	26.0	39.0
82-83	32.54794193685268	35.0	34.0	36.5	26.0	39.0
84-85	32.313503274787685	35.0	33.5	36.0	26.0	37.0
86-87	32.063981471782824	35.0	33.0	36.0	25.5	37.0
88-89	31.903617916536593	35.0	33.0	35.0	25.0	37.0
90-91	31.715330949362254	35.0	33.0	35.0	25.0	36.0
92-93	31.516669962684688	35.0	33.0	35.0	24.5	36.0
94-95	31.35824954896686	35.0	33.0	35.0	24.0	36.0
96-97	31.18964660033062	35.0	33.0	35.0	23.5	36.0
98-99	31.023577915705214	35.0	33.0	35.0	20.5	35.5
100	30.917643313504406	35.0	33.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.13344725038624716
1101	2	0.1814673768081505
1101	3	0.19473829428287104
1101	4	0.17785028777372958
1101	5	0.1543487751315098
1101	6	0.14944386925187558
1101	7	0.14996309711462175
1101	8	0.26568153606849165
1101	9	0.31019273804520964
1101	10-11	0.28684099288678766
1101	12-13	0.3003227658866763
1101	14-15	0.3883075949980608
1101	16-17	0.3460554142077612
1101	18-19	0.2973987703771144
1101	20-21	0.29059801376579486
1101	22-23	0.2749025241545553
1101	24-25	0.27261685557746773
1101	26-27	0.2675463309386146
1101	28-29	0.33136235466922415
1101	30-31	0.32172840601931085
1101	32-33	0.3400647832734478
1101	34-35	0.3711507817869162
1101	36-37	0.298774446867597
1101	38-39	0.2501884379674948
1101	40-41	0.3808593328922143
1101	42-43	0.3559432000195528
1101	44-45	0.3525332764987823
1101	46-47	0.3775718932823011
1101	48-49	0.39600808101514673
1101	50-51	0.32078595855912084
1101	52-53	0.3140824050291755
1101	54-55	0.34387063916918237
1101	56-57	0.3082918947084252
1101	58-59	0.30373403691454826
1101	60-61	0.3142207586212251
1101	62-63	0.32499848033761225
1101	64-65	0.32526763299109973
1101	66-67	0.2869162826160121
1101	68-69	0.2633755752239395
1101	70-71	0.3142251229932782
1101	72-73	0.3679314407585039
1101	74-75	0.29193473212143317
1101	76-77	0.3197523487776337
1101	78-79	0.30392202192720674
1101	80-81	0.37847347813524124
1101	82-83	0.3196631467058637
1101	84-85	0.33542076851856706
1101	86-87	0.3586646846508117
1101	88-89	0.39646038175874665
1101	90-91	0.44563371921132955
1101	92-93	0.41486405703253837
1101	94-95	0.4080004489079414
1101	96-97	0.4900578240191038
1101	98-99	0.5106401956769595
1101	100	0.5256945995315334
1102	1	0.12908184626729025
1102	2	0.23034538660111537
1102	3	0.21707445478676846
1102	4	0.1704725429862819
1102	5	0.14879643108982066
1102	6	0.13014861928113675
1102	7	0.1500017242836762
1102	8	0.21157946866942012
1102	9	0.25174484711979517
1102	10-11	0.28760118714406957
1102	12-13	0.23288304407350324
1102	14-15	0.36370070877065075
1102	16-17	0.3462159305657977
1102	18-19	0.36540123397167434
1102	20-21	0.3311951461880085
1102	22-23	0.29663911801256404
1102	24-25	0.2946928612077855
1102	26-27	0.30265540151463455
1102	28-29	0.32151518564465675
1102	30-31	0.29684443215583656
1102	32-33	0.3171135251536228
1102	34-35	0.33955631633041605
1102	36-37	0.3429044077108614
1102	38-39	0.3479481956665609
1102	40-41	0.4085610652851486
1102	42-43	0.3709990069335447
1102	44-45	0.25132760150903266
1102	46-47	0.280421359021382
1102	48-49	0.25883499347165895
1102	50-51	0.17000709598683983
1102	52-53	0.14408367776769637
1102	54-55	0.1855022721505506
1102	56-57	0.22728179868786924
1102	58-59	0.22076127574393922
1102	60-61	0.21382834574485088
1102	62-63	0.20268089914601717
1102	64-65	0.25640037472352617
1102	66-67	0.2815326431801708
1102	68-69	0.30387718550517917
1102	70-71	0.268892134990395
1102	72-73	0.2504057200925658
1102	74-75	0.2680057794116806
1102	76-77	0.258865049773533
1102	78-79	0.270013197176624
1102	80-81	0.2862093030099899
1102	82-83	0.25473114253875906
1102	84-85	0.24494855137691474
1102	86-87	0.215541210768869
1102	88-89	0.190707425645531
1102	90-91	0.19783502283378596
1102	92-93	0.1618075265266441
1102	94-95	0.22005515174352297
1102	96-97	0.24668816868648236
1102	98-99	0.27440205917975646
1102	100	0.2500356347129653
1103	1	0.13257778031816514
1103	2	0.2125281770008698
1103	3	0.17629872566389082
1103	4	0.1752687356246625
1103	5	0.14098287045944602
1103	6	0.14082991573534542
1103	7	0.15700200518114116
1103	8	0.16536432124448908
1103	9	0.2736854183601807
1103	10-11	0.26079895116425433
1103	12-13	0.2886776364825394
1103	14-15	0.37756461079998616
1103	16-17	0.36986407456819137
1103	18-19	0.3730212360806533
1103	20-21	0.2991535045189053
1103	22-23	0.3040582405412735
1103	24-25	0.2941395753945457
1103	26-27	0.32431144686615454
1103	28-29	0.32849433822310203
1103	30-31	0.37922146822900515
1103	32-33	0.4378244603366923
1103	34-35	0.3717407507574251
1103	36-37	0.45224323389337684
1103	38-39	0.39687707979975784
1103	40-41	0.3966238219155471
1103	42-43	0.4052148104281059
1103	44-45	0.49882643238730395
1103	46-47	0.4780846183131189
1103	48-49	0.4661996511755504
1103	50-51	0.3934969959439272
1103	52-53	0.3967068003174674
1103	54-55	0.385015222654161
1103	56-57	0.45909240315172894
1103	58-59	0.44293926121715543
1103	60-61	0.46876341373243946
1103	62-63	0.4798657567816065
1103	64-65	0.44737703480252833
1103	66-67	0.41917946733693157
1103	68-69	0.4154565446916081
1103	70-71	0.5168784263718749
1103	72-73	0.46895059799079064
1103	74-75	0.46848517390616706
1103	76-77	0.4032585186697588
1103	78-79	0.441254080692282
1103	80-81	0.48855168221797385
1103	82-83	0.44067303336790076
1103	84-85	0.45796920870781577
1103	86-87	0.4234258242021909
1103	88-89	0.4466369053532908
1103	90-91	0.43386968560719197
1103	92-93	0.40861419624246764
1103	94-95	0.42084665453727865
1103	96-97	0.4332230652842348
1103	98-99	0.49633800017150875
1103	100	0.48005694670066035
1104	1	0.11258855092096098
1104	2	0.15465388858163465
1104	3	0.12993005504384314
1104	4	0.09103991018562141
1104	5	0.1361972333354231
1104	6	0.12315171371050582
1104	7	0.17674601601482465
1104	8	0.17240427442706618
1104	9	0.26048275996361525
1104	10-11	0.2764258322195232
1104	12-13	0.25195532473263427
1104	14-15	0.3894758674315568
1104	16-17	0.2813069709770204
1104	18-19	0.2985555712573742
1104	20-21	0.2557527948793208
1104	22-23	0.2880817706109866
1104	24-25	0.26071807291550897
1104	26-27	0.287049292597203
1104	28-29	0.2927586875960486
1104	30-31	0.2932838734496599
1104	32-33	0.26848979916498905
1104	34-35	0.26369500258380896
1104	36-37	0.2716614522830696
1104	38-39	0.30221831212627137
1104	40-41	0.28405246336523504
1104	42-43	0.27027932254432585
1104	44-45	0.28817746543741407
1104	46-47	0.38132792470919696
1104	48-49	0.3250617766920314
1104	50-51	0.3327052783089002
1104	52-53	0.3388478987689112
1104	54-55	0.3839307531156422
1104	56-57	0.37594459579734263
1104	58-59	0.38510467485106403
1104	60-61	0.34184097201359975
1104	62-63	0.321248066534622
1104	64-65	0.2850724168006593
1104	66-67	0.28831130082549805
1104	68-69	0.3029882234062242
1104	70-71	0.3227060594844815
1104	72-73	0.3454754456581952
1104	74-75	0.34024123870479883
1104	76-77	0.3294612785225368
1104	78-79	0.3191112928885076
1104	80-81	0.27703810540751306
1104	82-83	0.2903648319715586
1104	84-85	0.30909608876072525
1104	86-87	0.29745040355522434
1104	88-89	0.2510845562741544
1104	90-91	0.33977455895050923
1104	92-93	0.3412823352125507
1104	94-95	0.3342729588773423
1104	96-97	0.35844166476816497
1104	98-99	0.36407608698764093
1104	100	0.3943519530442039
1105	1	0.13615480666694424
1105	2	0.16589029563733249
1105	3	0.1342140313372937
1105	4	0.11631372627022785
1105	5	0.12562785960160738
1105	6	0.15674255949767968
1105	7	0.16488284374172935
1105	8	0.20469766823776325
1105	9	0.2173824186322122
1105	10-11	0.17861572054117403
1105	12-13	0.12516384072611686
1105	14-15	0.21926419571143896
1105	16-17	0.24547560464490203
1105	18-19	0.1804354602137579
1105	20-21	0.122785205403936
1105	22-23	0.1854966667022211
1105	24-25	0.24814576246053832
1105	26-27	0.26092967569100267
1105	28-29	0.2966354060113048
1105	30-31	0.2641972038423077
1105	32-33	0.2603915139960691
1105	34-35	0.2538261854692152
1105	36-37	0.2609295909262741
1105	38-39	0.2782900148058616
1105	40-41	0.24436987238291152
1105	42-43	0.33444834363714904
1105	44-45	0.3326843399415651
1105	46-47	0.24548896444691337
1105	48-49	0.2440306374029646
1105	50-51	0.237685459215065
1105	52-53	0.26969843982489294
1105	54-55	0.29116953105134513
1105	56-57	0.36811681553464837
1105	58-59	0.2604631884497337
1105	60-61	0.3369206653223955
1105	62-63	0.3122903905607046
1105	64-65	0.34570318433842573
1105	66-67	0.2997088706313349
1105	68-69	0.3276157022884547
1105	70-71	0.29823712371748456
1105	72-73	0.2932200223013055
1105	74-75	0.28713198499401926
1105	76-77	0.3234503806807112
1105	78-79	0.26087662812088297
1105	80-81	0.23318567793335632
1105	82-83	0.26180886426456595
1105	84-85	0.20263179735551518
1105	86-87	0.20518357986837543
1105	88-89	0.1726539114998964
1105	90-91	0.15672340412510977
1105	92-93	0.19959858805786723
1105	94-95	0.24565127185735136
1105	96-97	0.2620940320288554
1105	98-99	0.2761175088556165
1105	100	0.3205898389390107
1106	1	0.08425546042291643
1106	2	0.12729903217229577
1106	3	0.0951036550357216
1106	4	0.11618322736663345
1106	5	0.07352617717761945
1106	6	0.12112371086676887
1106	7	0.11941526510602785
1106	8	0.16247799352267833
1106	9	0.18077386786422522
1106	10-11	0.1729191870447906
1106	12-13	0.1790729571492875
1106	14-15	0.2596540029983174
1106	16-17	0.15104572736495214
1106	18-19	0.1910792468078668
1106	20-21	0.17485763440431157
1106	22-23	0.19346538055262386
1106	24-25	0.16625191555059615
1106	26-27	0.2440192911164445
1106	28-29	0.24610443122107029
1106	30-31	0.2193029787660734
1106	32-33	0.23645822789901416
1106	34-35	0.2604007569926523
1106	36-37	0.2592710940004537
1106	38-39	0.2772051326307974
1106	40-41	0.2608758844838448
1106	42-43	0.3521619978157915
1106	44-45	0.3757029891152044
1106	46-47	0.3161756545145167
1106	48-49	0.27801888820649623
1106	50-51	0.33082926931638923
1106	52-53	0.32208775189310046
1106	54-55	0.2940173963388659
1106	56-57	0.306783772233274
1106	58-59	0.3373980673492696
1106	60-61	0.2964450034936732
1106	62-63	0.3405900113225684
1106	64-65	0.3661728708845118
1106	66-67	0.34541079588969126
1106	68-69	0.36044295784253677
1106	70-71	0.3715984802312491
1106	72-73	0.3798270949201026
1106	74-75	0.40397197755064695
1106	76-77	0.4238101803783394
1106	78-79	0.39860193994361737
1106	80-81	0.3792279586494445
1106	82-83	0.35569111048947377
1106	84-85	0.32576450702081416
1106	86-87	0.3452982598670715
1106	88-89	0.31382126008218236
1106	90-91	0.2918328368876857
1106	92-93	0.3576333987806706
1106	94-95	0.3712380574203529
1106	96-97	0.40293054799408523
1106	98-99	0.46430959078191947
1106	100	0.5479864711347453
1107	1	0.09366381781487831
1107	2	0.12859408482456303
1107	3	0.06710387540004348
1107	4	0.11429000549966872
1107	5	0.08334513177001668
1107	6	0.05934095598666289
1107	7	0.023628571026243606
1107	8	0.022250673480790795
1107	9	0.05952763640335945
1107	10-11	0.029804382291139575
1107	12-13	0.018975108643388694
1107	14-15	0.10018635710954271
1107	16-17	0.08809606281155169
1107	18-19	0.0832165127949196
1107	20-21	0.06916859957194532
1107	22-23	0.10165010694389309
1107	24-25	0.10867657334409841
1107	26-27	0.11518668986363423
1107	28-29	0.1323896564864313
1107	30-31	0.10860335389136822
1107	32-33	0.05977524690187863
1107	34-35	0.10590124915838572
1107	36-37	0.11314385646401348
1107	38-39	0.06445867733899036
1107	40-41	0.10632024558376685
1107	42-43	0.10941558187475664
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2216	64-65	-1.0806874686369738
2216	66-67	-0.9705531937894776
2216	68-69	-0.8419371349898697
2216	70-71	-0.9063761599429725
2216	72-73	-0.7965627930629964
2216	74-75	-0.7471643546987963
2216	76-77	-0.7659630926219023
2216	78-79	-0.6971234524030763
2216	80-81	-0.7602612161012132
2216	82-83	-0.7396597285134163
2216	84-85	-0.759358147009003
2216	86-87	-0.791156548427665
2216	88-89	-0.8281474169811815
2216	90-91	-0.8330672089169795
2216	92-93	-0.7667911857273104
2216	94-95	-0.8568147827724069
2216	96-97	-1.103372205834006
2216	98-99	-1.1793625741991
2216	100	-1.1616137404333813
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	223.0
3	610.0
4	477.0
5	419.0
6	728.0
7	1623.0
8	2907.0
9	4801.0
10	6404.0
11	8390.0
12	11739.0
13	16656.0
14	17666.0
15	21742.0
16	27379.0
17	38525.0
18	42555.0
19	24644.0
20	16890.0
21	15769.0
22	15767.0
23	16410.0
24	18849.0
25	22752.0
26	28662.0
27	36906.0
28	46008.0
29	59233.0
30	74385.0
31	93345.0
32	118265.0
33	150086.0
34	202909.0
35	307641.0
36	467799.0
37	871618.0
38	1364617.0
39	699992.0
40	4008.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.05999489405157	21.375311915801262	14.59539476393224	23.969298426214927
2	13.869225391864305	22.182290443735944	39.67326000602132	24.275224158378432
3	15.947461761692919	27.963610277330876	32.087828235372555	24.001099725603645
4	11.145468949138204	18.92655826787074	38.62567366565756	31.302299117333504
5	11.497204981662549	38.251260860626516	35.44996574525624	14.80156841245469
6	27.499861608400543	39.64661061995526	18.52107637179001	14.332451399854179
7	23.99339918372622	34.697850495503666	23.613146399379843	17.69560392139028
8	24.617406391201875	35.06069371953198	21.45401108244044	18.8678888068257
9	24.882212800389514	16.28540895695126	22.898284335161613	35.93409390749762
10-11	22.687308172240723	27.55389082727583	29.547993959888867	20.210807040594577
12-13	24.04049348489391	25.57518944215118	29.882337301382332	20.501979771572575
14-15	21.449823321772918	27.08510867290379	28.492875353515938	22.97219265180735
16-17	19.427628807595344	30.293077394961802	29.132193096306764	21.147100701136086
18-19	19.31704928942859	27.78382676540864	33.05037310169426	19.8487508434685
20-21	20.6435199085319	26.98550787864919	32.98132135270226	19.389650860116653
22-23	20.28045031906209	27.17173461162584	32.93783860926012	19.609976460051953
24-25	20.147681908101948	26.972722556433837	33.039844934408066	19.839750601056146
26-27	19.852146325090818	27.01448265515962	33.0884436532172	20.044927366532363
28-29	19.895743922939978	27.003470195072172	33.13943689535766	19.96134898663019
30-31	20.179027190862044	27.2679013814191	32.79137579267065	19.761695635048195
32-33	19.860620437637007	27.14996782005158	33.04242248388044	19.946989258430968
34-35	20.147014911397786	26.837315968977443	32.82332877781032	20.192340341814454
36-37	20.413259452479075	27.070325724016936	32.34272278730443	20.17369203619956
38-39	20.252150454765182	27.13399670447785	32.484682530748636	20.12917031000833
40-41	20.148149763975123	26.995469240212582	32.48169838719851	20.374682608613785
42-43	20.105317660016393	27.16557470532272	32.32294411189762	20.40616352276326
44-45	20.452385161210266	27.265789452563986	31.924328502351834	20.35749688387391
46-47	20.48108670798818	27.541585350457215	31.422167898804236	20.555160042750366
48-49	20.516929930596113	27.464017029325948	31.140194370395246	20.878858669682685
50-51	20.539027040664767	27.522439052548297	30.869176498527214	21.06935740825972
52-53	20.500806175292453	27.767084794000862	30.708950655075302	21.023158375631386
54-55	20.605253726809785	28.036524634310695	30.60370928172397	20.754512357155548
56-57	20.47755620236122	27.779855211661932	30.515962546919766	21.226626039057084
58-59	20.419358105062724	27.413177315316183	30.87297480965958	21.294489769961512
60-61	20.688817114240702	27.681076359056622	30.586416482056737	21.043690044645935
62-63	20.477483341630194	27.589849419737828	30.756858696236	21.175808542395984
64-65	20.439961072894892	27.48072320124198	30.887565160611352	21.191750565251777
66-67	20.403398036874556	27.56898288140455	30.636176033472484	21.391443048248405
68-69	20.76191665842656	27.71063535378656	30.386016604849587	21.14143138293729
70-71	20.775169566542036	27.443104571770586	30.43165731892915	21.350068542758223
72-73	20.564024481216713	27.559087039364332	30.66693021091703	21.20995826850193
74-75	20.510177990722074	27.65639461392471	30.390250201543097	21.44317719381012
76-77	20.57122701799132	27.568995672098545	30.173000817590818	21.686776492319318
78-79	20.564411367867415	27.59575405297422	30.415434037811746	21.424400541346618
80-81	20.474640999843807	27.5550639081088	30.3521999325431	21.618095159504293
82-83	20.276955506168385	27.429310565341584	30.547225943294933	21.746507985195098
84-85	20.37597105052956	27.42535107735818	30.30976176200854	21.88891611010372
86-87	20.47824226823111	27.46710035541432	30.16244395654689	21.89221341980768
88-89	20.218024903902727	27.380371523309776	30.30761622990827	22.09398734287923
90-91	20.188432767097332	27.364299576964147	30.11074003184344	22.33652762409508
92-93	20.240793622326425	27.374199989525433	30.15265600750876	22.232350380639378
94-95	20.12279707840414	27.191531298417765	30.326250221478006	22.359421401700086
96-97	20.094312443151182	27.28896484736736	30.131241022517273	22.48548168696418
98-99	19.995563213608012	27.171694784491745	30.1829577601884	22.649784241711842
100	20.034296506946248	27.22876362800493	30.02444547017967	22.712494394869147
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5516.0
1	3346.0
2	6509.5
3	10817.0
4	6299.5
5	2976.0
6	5577.5
7	12482.0
8	19445.5
9	20831.5
10	14268.0
11	7379.0
12	5947.5
13	6134.5
14	6612.5
15	7140.0
16	7683.0
17	8408.0
18	9191.0
19	10011.5
20	11385.5
21	13376.5
22	16541.5
23	22193.0
24	28998.0
25	35441.0
26	44973.0
27	58935.0
28	76806.0
29	94288.5
30	103837.5
31	111509.0
32	121015.0
33	131312.5
34	141244.0
35	147252.0
36	151689.0
37	155797.0
38	158291.5
39	159698.5
40	162760.0
41	162463.0
42	158185.5
43	157074.0
44	155950.5
45	153900.0
46	152631.5
47	150124.0
48	145659.0
49	140344.5
50	137204.0
51	131418.0
52	121132.0
53	112938.5
54	108316.0
55	103669.0
56	95172.0
57	85143.5
58	77342.5
59	71586.5
60	63021.0
61	52266.0
62	44414.5
63	36551.0
64	28450.5
65	22253.0
66	17354.5
67	13397.5
68	10361.5
69	7951.0
70	5770.5
71	4199.5
72	3295.5
73	2595.5
74	1994.5
75	1454.0
76	926.5
77	673.5
78	484.5
79	269.5
80	140.5
81	65.5
82	38.5
83	19.5
84	8.5
85	5.0
86	6.0
87	3.5
88	1.0
89	2.0
90	2.0
91	0.5
92	0.5
93	0.5
94	1.5
95	2.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0477219508009118
2	0.0
3	1.0289338249441958E-4
4	0.06828004862329683
5	0.004486151476756693
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.011277114721388386
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	7.40832353959821E-4
26-27	0.0
28-29	3.704161769799105E-4
30-31	0.003971684564284595
32-33	1.3376139724274546E-4
34-35	0.001975552943892856
36-37	0.004609623535749997
38-39	0.0016051367669129455
40-41	0.001224431251683593
42-43	0.0020578676498883915
44-45	0.0
46-47	5.247562507215399E-4
48-49	3.4983750048102655E-4
50-51	0.0014302180166724321
52-53	6.996750009620531E-4
54-55	9.980658101958698E-4
56-57	0.006297075008658478
58-59	0.009733713983972091
60-61	0.0011112485309397314
62-63	0.002675227944854909
64-65	0.001234720589933035
66-67	1.0289338249441958E-4
68-69	7.30543015710379E-4
70-71	4.8359889772377196E-4
72-73	0.0
74-75	3.3954816223158457E-4
76-77	0.0
78-79	7.30543015710379E-4
80-81	0.0
82-83	0.0012861672811802448
84-85	7.30543015710379E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	1.9549742673939717E-4
94-95	0.0
96-97	3.9099485347879434E-4
98-99	5.350455889709818E-4
100	2.46944117986607E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4859399.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.22420783641737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.73475500087838	36.233317914230575
2	16.83143110512843	17.24353450226878
3	5.6185625760986335	8.634192514199789
4	2.3339317630383856	4.782152228235772
5	1.2356904431613651	3.1648632040986233
6	0.7446343657050953	2.288598330660997
7	0.49553757929724707	1.7768463966874137
8	0.33187595959890454	1.3600066504323793
9	0.24260926746922212	1.1184720785895967
>10	1.2946388769231663	11.992899299676269
>50	0.0878472492422898	3.097203255662699
>100	0.04502594135167317	4.160485293510477
>500	0.0022127100860003792	0.7364081564646029
>1k	9.253137578053565E-4	0.6426201350050244
>5k	1.6092413179206563E-4	0.6477309259316184
>10k+	1.6092413179206563E-4	2.120669114345493
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34625	0.7125366737738555	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30139	0.6202207309998623	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21108	0.4343747035384417	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17033	0.35051659680548974	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9034	0.1859077634909173	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8161	0.16794257890739162	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7440	0.15310535315169632	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6796	0.13985268548641508	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.0578676498883915E-5	0.0	0.0	0.0
6	0.0	2.0578676498883915E-5	0.0	0.0	0.0
7	0.0	2.0578676498883915E-5	0.0	0.0	0.0
8	0.0	2.0578676498883915E-5	0.0	0.0	0.0
9	0.0	2.0578676498883915E-5	0.0	0.0	0.0
10-11	2.0578676498883915E-5	2.0578676498883915E-5	0.0	0.0	0.0
12-13	2.0578676498883915E-5	2.0578676498883915E-5	0.0	0.0	0.0
14-15	2.0578676498883915E-5	2.0578676498883915E-5	0.0	0.0	0.0
16-17	2.0578676498883915E-5	2.0578676498883915E-5	0.0	0.0	0.0
18-19	2.0578676498883915E-5	2.0578676498883915E-5	0.0	2.0578676498883915E-5	0.0
20-21	2.0578676498883915E-5	2.0578676498883915E-5	0.0	1.3376139724274546E-4	0.0
22-23	2.0578676498883915E-5	2.0578676498883915E-5	0.0	5.144669124720979E-4	0.0
24-25	2.0578676498883915E-5	2.0578676498883915E-5	0.0	0.0011935632369352671	0.0
26-27	2.0578676498883915E-5	2.0578676498883915E-5	0.0	0.0025517558858616056	0.0
28-29	2.0578676498883915E-5	2.0578676498883915E-5	0.0	0.005700293390190844	0.0
30-31	2.0578676498883915E-5	2.0578676498883915E-5	0.0	0.013900895974996084	0.0
32-33	2.0578676498883915E-5	3.086801474832587E-5	0.0	0.03141334967554629	0.0
34-35	2.0578676498883915E-5	4.115735299776783E-5	0.0	0.0583405478743359	0.0
36-37	2.0578676498883915E-5	4.115735299776783E-5	0.0	0.09991976374033085	0.0
38-39	2.0578676498883915E-5	4.115735299776783E-5	0.0	0.15455614984486765	0.0
40-41	2.0578676498883915E-5	4.115735299776783E-5	0.0	0.21466646389810756	0.0
42-43	2.0578676498883915E-5	4.115735299776783E-5	0.0	0.27664943751274595	0.0
44-45	2.0578676498883915E-5	4.115735299776783E-5	0.0	0.3561757328426828	0.0
46-47	2.0578676498883915E-5	4.115735299776783E-5	0.0	0.4526382789312011	0.0
48-49	2.0578676498883915E-5	4.115735299776783E-5	0.0	0.5580319706202352	0.0
50-51	2.0578676498883915E-5	6.173602949665175E-5	0.0	0.6612649012768863	0.0
52-53	2.0578676498883915E-5	6.173602949665175E-5	0.0	0.7652077962727489	0.0
54-55	2.0578676498883915E-5	6.173602949665175E-5	0.0	0.885130033569995	0.0
56-57	2.0578676498883915E-5	6.173602949665175E-5	0.0	1.0328437734789837	0.0
58-59	2.0578676498883915E-5	6.173602949665175E-5	0.0	1.198656459368741	0.0
60-61	2.0578676498883915E-5	6.173602949665175E-5	0.0	1.369871047839455	0.0
62-63	2.0578676498883915E-5	6.173602949665175E-5	0.0	1.5423718035913494	0.0
64-65	2.0578676498883915E-5	6.173602949665175E-5	0.0	1.7355438398863727	0.0
66-67	2.0578676498883915E-5	6.173602949665175E-5	0.0	1.9604687740191742	0.0
68-69	4.115735299776783E-5	6.173602949665175E-5	0.0	2.211291972525821	0.0
70-71	4.115735299776783E-5	6.173602949665175E-5	0.0	2.484473903048505	0.0
72-73	4.115735299776783E-5	6.173602949665175E-5	0.0	2.776526479920665	0.0
74-75	4.115735299776783E-5	6.173602949665175E-5	0.0	3.0740735634180276	0.0
76-77	5.144669124720979E-5	6.173602949665175E-5	0.0	3.401480306515271	0.0
78-79	6.173602949665175E-5	6.173602949665175E-5	0.0	3.7704559761402594	0.0
80-81	6.173602949665175E-5	6.173602949665175E-5	0.0	4.172594182943199	0.0
82-83	6.173602949665175E-5	6.173602949665175E-5	0.0	4.5945084978615665	0.0
84-85	6.173602949665175E-5	6.173602949665175E-5	0.0	5.015702559102474	0.0
86-87	6.173602949665175E-5	6.173602949665175E-5	0.0	5.46622123435429	0.0
88	6.173602949665175E-5	6.173602949665175E-5	0.0	5.827078616100469	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12725	0.0	58.1204	1
GTATCAA	23175	0.0	40.398678	1
GTGGTAT	4420	0.0	35.870834	1
TATAACG	460	0.0	35.777622	2
TACCTGG	3925	0.0	32.825565	2
ATAACGC	520	0.0	32.553707	3
TGGTATC	5090	0.0	30.947695	2
ATCAACG	30360	0.0	30.712997	3
TCAACGC	30650	0.0	30.376377	4
TATCAAC	30980	0.0	30.265303	2
CAACGCA	31925	0.0	29.169548	5
AACGCAG	33355	0.0	28.086662	6
GTACCTG	4875	0.0	26.24993	1
ACGCAGA	37595	0.0	24.75648	7
CGCAGAG	37410	0.0	24.740688	8
ACCTGGG	4965	0.0	24.339697	3
GCAGAGT	39480	0.0	23.086304	9
GTACATG	18750	0.0	22.256468	1
TACATGG	18890	0.0	21.357805	2
AGAGTAC	38015	0.0	21.17529	10-11
>>END_MODULE