FastQCFastQC Report
Wed 25 May 2016
SRR2431432_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431432_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2321354
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA429631.850773298686887No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60570.26092530480056036No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37220.16033745822481188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG25200.050.1750872
GTACCTG29750.043.454851
GGTATCA44700.037.2295881
TCTGTCG6700.033.6717268
GTATCAA70550.032.450731
ACCTGGG38550.032.189633
GTCTCGC8150.029.4174541
TCGCTCT7600.027.8314174
GTACATG121350.027.3112681
TATAACG1755.5551936E-926.8595262
TACATGG121250.026.7875292
ATCAACG80000.026.6748643
TATCAAC84750.026.5663682
TCAACGC80600.026.534614
CGCTCTG7100.025.1566185
CAACGCA88050.024.2889695
TCTCGCT8950.024.1585672
CTGTCGC9200.024.010939
ACATGGG131050.023.4213833
CATGGGT55150.023.3529324
Produced by FastQC (version 0.11.5)