##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431432_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2321354 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.10214900441725 31.0 30.0 34.0 20.0 34.0 2 29.164990776934495 31.0 30.0 34.0 18.0 34.0 3 29.20053554951119 31.0 30.0 34.0 18.0 34.0 4 32.3012939861822 37.0 33.0 37.0 18.0 37.0 5 32.59979735964441 37.0 35.0 37.0 20.0 37.0 6 32.451528719876414 37.0 35.0 37.0 17.0 37.0 7 32.38680744082979 37.0 35.0 37.0 17.0 37.0 8 32.2438818034647 37.0 33.0 37.0 17.0 37.0 9 33.400414585625455 39.0 34.0 39.0 13.0 39.0 10-11 33.39192062046547 39.0 34.0 39.0 13.0 39.0 12-13 33.365106528345095 39.0 34.0 39.0 12.5 39.0 14-15 34.4738458675411 40.0 34.0 41.0 12.5 41.0 16-17 34.486647447998024 40.0 34.0 41.0 13.0 41.0 18-19 34.45004144133122 40.0 34.0 41.0 13.0 41.0 20-21 34.545564355975 40.0 34.0 41.0 16.5 41.0 22-23 34.52183962463286 40.0 34.0 41.0 17.5 41.0 24-25 34.57364581188392 40.0 34.0 41.0 18.0 41.0 26-27 34.58350772867904 40.0 34.0 41.0 19.0 41.0 28-29 34.491035835120364 40.0 34.0 41.0 18.5 41.0 30-31 34.46439082535451 40.0 34.0 41.0 19.0 41.0 32-33 34.38783162757598 40.0 34.0 41.0 18.5 41.0 34-35 34.27960255092502 39.5 33.0 41.0 17.5 41.0 36-37 34.14254740983064 39.0 33.0 41.0 16.0 41.0 38-39 34.01919720128856 39.0 33.0 41.0 15.5 41.0 40-41 33.92300312662351 39.0 33.0 41.0 14.5 41.0 42-43 33.69566016212951 39.0 33.0 41.0 9.0 41.0 44-45 33.55618940497658 39.0 33.0 41.0 9.0 41.0 46-47 33.40948752322998 38.5 32.5 41.0 8.0 41.0 48-49 33.26808664253707 38.0 32.0 40.5 6.5 41.0 50-51 33.105430279052655 38.0 32.0 40.0 2.0 41.0 52-53 32.996879838232346 38.0 32.0 40.0 2.0 41.0 54-55 32.92368613317917 38.0 32.0 40.0 2.0 41.0 56-57 32.77585258431071 38.0 31.5 40.0 2.0 41.0 58-59 32.572700243047805 37.5 31.0 40.0 2.0 41.0 60-61 32.35526830461877 37.0 31.0 40.0 2.0 41.0 62-63 32.06533428335359 36.5 31.0 40.0 2.0 41.0 64-65 31.90645394885916 36.0 31.0 40.0 2.0 41.0 66-67 31.657502690240264 36.0 31.0 39.5 2.0 41.0 68-69 31.381338649770782 35.0 31.0 39.0 2.0 41.0 70-71 31.073731106931557 35.0 30.0 39.0 2.0 41.0 72-73 30.763740041372408 35.0 30.0 38.5 2.0 40.5 74-75 30.44071111084307 35.0 30.0 37.5 2.0 40.0 76-77 30.131552964347534 35.0 30.0 37.0 2.0 39.0 78-79 29.80175837033042 35.0 30.0 36.5 2.0 39.0 80-81 29.511795055816563 35.0 29.0 36.0 2.0 39.0 82-83 29.225924826631353 35.0 29.0 36.0 2.0 37.5 84-85 28.94163686365802 34.5 29.0 35.0 2.0 37.0 86-87 28.69279954716084 34.0 29.0 35.0 2.0 37.0 88-89 28.437707045112464 34.0 29.0 35.0 2.0 36.0 90-91 28.224578629541206 34.0 28.0 35.0 2.0 36.0 92-93 28.031349376269194 34.0 28.0 35.0 2.0 36.0 94-95 27.804138231394262 34.0 28.0 35.0 2.0 35.5 96-97 27.712612768237847 34.0 28.0 35.0 2.0 35.0 98-99 27.55676450037349 34.0 27.5 35.0 2.0 35.0 100 27.421440676432805 34.0 27.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4138786389751097 1101 2 0.5973584179552667 1101 3 0.5333959339402412 1101 4 0.4956490652371528 1101 5 0.3218826087644544 1101 6 0.4231116649996949 1101 7 0.5584535301152087 1101 8 0.5232798614913747 1101 9 0.6110749523790489 1101 10-11 0.6953842288010748 1101 12-13 0.637444503471869 1101 14-15 0.6224132651705929 1101 16-17 0.5220031426510445 1101 18-19 0.5567987712737406 1101 20-21 0.5386428092809155 1101 22-23 0.5529733680011191 1101 24-25 0.48204247181825366 1101 26-27 0.5696984576994168 1101 28-29 0.6277689994257543 1101 30-31 0.6368168535188516 1101 32-33 0.6351354403044311 1101 34-35 0.5522565222292428 1101 36-37 0.5690445247595406 1101 38-39 0.6054888048561153 1101 40-41 0.5122636358913297 1101 42-43 0.5043254215258415 1101 44-45 0.46498392388353693 1101 46-47 0.4170038112604644 1101 48-49 0.4421119145228971 1101 50-51 0.3808464190922294 1101 52-53 0.38644038701027483 1101 54-55 0.4085836678890118 1101 56-57 0.4051613067375541 1101 58-59 0.409599612986419 1101 60-61 0.45146234956750675 1101 62-63 0.5152094546515329 1101 64-65 0.4807301764500984 1101 66-67 0.5702369660721693 1101 68-69 0.4944268729660486 1101 70-71 0.47330727096873915 1101 72-73 0.48826021948054077 1101 74-75 0.46133676313177574 1101 76-77 0.5170629712219075 1101 78-79 0.47528234585988116 1101 80-81 0.5105038858053419 1101 82-83 0.5892208521544795 1101 84-85 0.5256986716235623 1101 86-87 0.4736238711450049 1101 88-89 0.5558834256066838 1101 90-91 0.4598334006330589 1101 92-93 0.39312483300037826 1101 94-95 0.37438010038633607 1101 96-97 0.5139665912054916 1101 98-99 0.44394679951322047 1101 100 0.39665532999309505 1102 1 0.25785899716962746 1102 2 0.37324010662293006 1102 3 0.2919869097709622 1102 4 0.3128001240966114 1102 5 0.1919251528966157 1102 6 0.22969929468872152 1102 7 0.23653404578202242 1102 8 0.15236664943832068 1102 9 0.33221187691383847 1102 10-11 0.36466810588244414 1102 12-13 0.2528101724283047 1102 14-15 0.31833986988966956 1102 16-17 0.223035882078932 1102 18-19 0.2575894013827664 1102 20-21 0.2545795901121011 1102 22-23 0.2906943469905414 1102 24-25 0.2793051323711566 1102 26-27 0.3176306937019078 1102 28-29 0.3243775309300432 1102 30-31 0.31192437088304104 1102 32-33 0.43698400425196127 1102 34-35 0.36237570827912435 1102 36-37 0.2851911625929233 1102 38-39 0.28612694369785885 1102 40-41 0.313491740473296 1102 42-43 0.4031407924882302 1102 44-45 0.2349792031857021 1102 46-47 0.155945171631366 1102 48-49 0.1420349139247179 1102 50-51 0.05247647142487466 1102 52-53 0.19178275909150955 1102 54-55 0.23974655047669557 1102 56-57 0.21641597681516345 1102 58-59 0.19548038759739228 1102 60-61 0.170485518011958 1102 62-63 0.1472481250345936 1102 64-65 0.07453162829609639 1102 66-67 0.19106021874867807 1102 68-69 0.15652050823891628 1102 70-71 0.19507984093746344 1102 72-73 0.2410168524659504 1102 74-75 0.205589631707781 1102 76-77 0.17954768391306786 1102 78-79 0.05232112616114648 1102 80-81 0.12814037818801438 1102 82-83 0.1473836387083587 1102 84-85 0.07979101333266314 1102 86-87 0.1673296941489184 1102 88-89 0.27031606252138474 1102 90-91 0.21607183752670522 1102 92-93 0.230217079832709 1102 94-95 0.28938353099149694 1102 96-97 0.2698949430455784 1102 98-99 0.28869456036359153 1102 100 0.30798488908223476 1103 1 0.19288119995481523 1103 2 0.22679434615015737 1103 3 0.20198905867124495 1103 4 0.08324281266167333 1103 5 0.003055134549768468 1103 6 0.10376146399789121 1103 7 0.15381148731418648 1103 8 0.2635468413362503 1103 9 0.29707716843538634 1103 10-11 0.28195522501913217 1103 12-13 0.247726691995382 1103 14-15 0.32006904538420144 1103 16-17 0.17892248094760532 1103 18-19 0.18405603526831982 1103 20-21 0.22134211699223982 1103 22-23 0.24607360334263717 1103 24-25 0.2848668752992367 1103 26-27 0.31688164711118105 1103 28-29 0.3854298084999854 1103 30-31 0.2585843415915221 1103 32-33 0.4164868378545634 1103 34-35 0.3267174649994402 1103 36-37 0.3332220580385439 1103 38-39 0.4013910847080169 1103 40-41 0.3031488832441198 1103 42-43 0.306741182387114 1103 44-45 0.23314333800220055 1103 46-47 0.19321627031989408 1103 48-49 0.21744394142081092 1103 50-51 0.12509070114797538 1103 52-53 0.2206465214571267 1103 54-55 0.1932047787254021 1103 56-57 0.1572092892410879 1103 58-59 0.1794182077136668 1103 60-61 0.2735524783490746 1103 62-63 0.22599540680791108 1103 64-65 0.2660361738433359 1103 66-67 0.2923523536225723 1103 68-69 0.2568500283128152 1103 70-71 0.3161120702874385 1103 72-73 0.32790309406579254 1103 74-75 0.19834280539556914 1103 76-77 0.18688297769304185 1103 78-79 0.2218275796599265 1103 80-81 0.2337727062589927 1103 82-83 0.2918173543873266 1103 84-85 0.31841269962645313 1103 86-87 0.2450846673990732 1103 88-89 0.36692990174134366 1103 90-91 0.3135988649518069 1103 92-93 0.21525101848368422 1103 94-95 0.31383326711862836 1103 96-97 0.2552324752318782 1103 98-99 0.21866986878176675 1103 100 0.23171328017151183 1104 1 -0.003064510269073395 1104 2 0.09423843268121246 1104 3 0.0671349729832933 1104 4 0.15072127729074936 1104 5 0.12547532357842783 1104 6 0.017649779514698594 1104 7 0.09663373292352873 1104 8 0.08530585159589776 1104 9 0.12495846006341083 1104 10-11 0.13662036199368544 1104 12-13 0.1275688798088197 1104 14-15 0.19660170530413978 1104 16-17 0.1460606896233827 1104 18-19 0.11264843749061271 1104 20-21 0.056149723200938695 1104 22-23 0.062179124479719405 1104 24-25 0.10809440452424468 1104 26-27 0.13468299694212504 1104 28-29 0.21867350197236135 1104 30-31 0.15113679544637648 1104 32-33 0.2225711070764973 1104 34-35 0.19092107055691798 1104 36-37 0.20368912774540604 1104 38-39 0.20500601803049534 1104 40-41 0.23121798249434278 1104 42-43 0.18250396914987022 1104 44-45 0.13931602362212203 1104 46-47 0.10022733090965374 1104 48-49 0.062442327903319494 1104 50-51 0.007406040611705578 1104 52-53 0.06045239785878209 1104 54-55 0.09126554146855881 1104 56-57 0.17078591242515273 1104 58-59 0.14335245760246806 1104 60-61 0.05679311922077801 1104 62-63 0.10345638439601146 1104 64-65 0.11362254790611459 1104 66-67 0.09153447468580822 1104 68-69 0.1560944006254381 1104 70-71 0.16879919470853366 1104 72-73 0.16742297085701452 1104 74-75 0.1799027352148279 1104 76-77 0.12913267897426195 1104 78-79 0.20975181847214586 1104 80-81 0.22633453595876318 1104 82-83 0.14926544563973465 1104 84-85 0.19657214132032763 1104 86-87 0.21112630196498472 1104 88-89 0.2827188245803214 1104 90-91 0.1961998406211265 1104 92-93 0.19111348719452792 1104 94-95 0.15921486400671725 1104 96-97 0.17340351680829968 1104 98-99 0.2182483191653759 1104 100 0.20401674356501331 1105 1 0.27722165072841776 1105 2 0.37043317415882626 1105 3 0.3745561276840128 1105 4 0.3839981328534634 1105 5 0.3002333095456322 1105 6 0.48700650014320956 1105 7 0.4705297978183296 1105 8 0.5245869414254827 1105 9 0.5948655170704669 1105 10-11 0.5649935516514688 1105 12-13 0.5954125668781245 1105 14-15 0.677664383450093 1105 16-17 0.6489021078383246 1105 18-19 0.5989438785200605 1105 20-21 0.6242602442475231 1105 22-23 0.5979410280485311 1105 24-25 0.6104907514599276 1105 26-27 0.5920471953945068 1105 28-29 0.6352170258720591 1105 30-31 0.658990354816062 1105 32-33 0.5796361960542171 1105 34-35 0.5819812188606548 1105 36-37 0.5373403007813948 1105 38-39 0.42109051650058404 1105 40-41 0.4743343409691576 1105 42-43 0.5017192082652073 1105 44-45 0.30990916548780945 1105 46-47 0.3161624573339097 1105 48-49 0.36288779936394633 1105 50-51 0.38937915747127505 1105 52-53 0.39525783315693275 1105 54-55 0.45038460482145126 1105 56-57 0.5156310718139139 1105 58-59 0.3294953425343152 1105 60-61 0.40406398667827403 1105 62-63 0.473800230667905 1105 64-65 0.46030040747168854 1105 66-67 0.5717786079399438 1105 68-69 0.4495308693971971 1105 70-71 0.4513668244899627 1105 72-73 0.471879521077863 1105 74-75 0.46991430393623546 1105 76-77 0.4438162120676914 1105 78-79 0.517746276482157 1105 80-81 0.5565714659482524 1105 82-83 0.43214204153693814 1105 84-85 0.4200738709738019 1105 86-87 0.40519138607092486 1105 88-89 0.5160135143127462 1105 90-91 0.4088852333399835 1105 92-93 0.3874819195657899 1105 94-95 0.41524511876217574 1105 96-97 0.4288639045944862 1105 98-99 0.45570202427068196 1105 100 0.5267571890608345 1106 1 0.3294934736799888 1106 2 0.30694924264945556 1106 3 0.3388729189155164 1106 4 0.26891935382652576 1106 5 0.2938115490971569 1106 6 0.24301160156390722 1106 7 0.18936804822047293 1106 8 0.3786669066872008 1106 9 0.4327030796329723 1106 10-11 0.47702880156540317 1106 12-13 0.3785551656082333 1106 14-15 0.45150451132171554 1106 16-17 0.44874404222262854 1106 18-19 0.5124257645043571 1106 20-21 0.45634139370699245 1106 22-23 0.5071029942513618 1106 24-25 0.5585459578898977 1106 26-27 0.5751370263679334 1106 28-29 0.6679804810134371 1106 30-31 0.5767277392736005 1106 32-33 0.5809094293146941 1106 34-35 0.4863053909577175 1106 36-37 0.4995195183145853 1106 38-39 0.38605601927197597 1106 40-41 0.5014878799482076 1106 42-43 0.3675754990101936 1106 44-45 0.396282098149463 1106 46-47 0.4048279058522013 1106 48-49 0.4201737050341805 1106 50-51 0.4349904197758363 1106 52-53 0.5650327642880981 1106 54-55 0.5798354891333446 1106 56-57 0.6384467735699033 1106 58-59 0.6655814773033555 1106 60-61 0.6360896353558658 1106 62-63 0.6715437279352159 1106 64-65 0.7248067874728683 1106 66-67 0.7044657505671914 1106 68-69 0.6713506211230786 1106 70-71 0.6448576242104807 1106 72-73 0.5618493525537573 1106 74-75 0.6041051196814493 1106 76-77 0.6434996904041377 1106 78-79 0.5956497592729235 1106 80-81 0.7011202774387684 1106 82-83 0.6770064580241737 1106 84-85 0.673207513529416 1106 86-87 0.7158001241466216 1106 88-89 0.6930283734821643 1106 90-91 0.7109125693635647 1106 92-93 0.7171457421887197 1106 94-95 0.686834659797281 1106 96-97 0.6664034740462554 1106 98-99 0.82260666783327 1106 100 0.818398307883264 1107 1 0.17517317026906554 1107 2 0.19915590726020582 1107 3 0.21489271527389775 1107 4 0.24065441721802472 1107 5 0.32909941491022465 1107 6 0.3269656253587172 1107 7 0.33221696615102303 1107 8 0.410416092372202 1107 9 0.32122299180210234 1107 10-11 0.3795794340244427 1107 12-13 0.3153860276505043 1107 14-15 0.3738199850935615 1107 16-17 0.3622975229642975 1107 18-19 0.33818824592501073 1107 20-21 0.2388509596747781 1107 22-23 0.27447113213366237 1107 24-25 0.23070492170797507 1107 26-27 0.15317965597339622 1107 28-29 0.16259577756175503 1107 30-31 0.16244440265345617 1107 32-33 0.19219181165428267 1107 34-35 0.13369227032791997 1107 36-37 0.05446634132796646 1107 38-39 0.10388677145947156 1107 40-41 0.12563865627728177 1107 42-43 0.11217549199537302 1107 44-45 0.03377824330598855 1107 46-47 0.13576611850191256 1107 48-49 0.14913387010751222 1107 50-51 0.21019158304516594 1107 52-53 0.11736714499177481 1107 54-55 0.20339190815911934 1107 56-57 0.21559426881504606 1107 58-59 0.13783702728529335 1107 60-61 0.17475363263993415 1107 62-63 0.24520540238657418 1107 64-65 0.23824422584501193 1107 66-67 0.26998692209548025 1107 68-69 0.26836007326544475 1107 70-71 0.18627204403833986 1107 72-73 0.2562926205080629 1107 74-75 0.28244217329719135 1107 76-77 0.2489453764868621 1107 78-79 0.32254079988874906 1107 80-81 0.28916796301060543 1107 82-83 0.24818581181343546 1107 84-85 0.278704487229124 1107 86-87 0.36906092465698137 1107 88-89 0.37165181053340035 1107 90-91 0.3390623735441345 1107 92-93 0.36186773352475043 1107 94-95 0.44980546357681206 1107 96-97 0.4461728749936569 1107 98-99 0.41600290735901524 1107 100 0.4128188107533859 1108 1 0.07017823934901557 1108 2 0.14041860026506825 1108 3 0.14479719743624742 1108 4 0.14195801322301804 1108 5 0.1294378631888904 1108 6 0.17909978286743922 1108 7 0.15736412553926726 1108 8 0.284093919296005 1108 9 0.27668452332993354 1108 10-11 0.22925206987913072 1108 12-13 0.07325416978093813 1108 14-15 0.23139293254617144 1108 16-17 0.2296604993471334 1108 18-19 0.222155047034569 1108 20-21 0.2452289547397939 1108 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40-41 -0.5800058407372717 2215 42-43 -0.6461136176484601 2215 44-45 -0.38810269668262265 2215 46-47 -0.3523615977911376 2215 48-49 -0.3862033317310889 2215 50-51 -0.39684029767884255 2215 52-53 -0.4285194766345555 2215 54-55 -0.5790810413135716 2215 56-57 -0.4891436266836351 2215 58-59 -0.42244009795397375 2215 60-61 -0.43124307989288013 2215 62-63 -0.3967011860008789 2215 64-65 -0.40422744890128115 2215 66-67 -0.36865623257428837 2215 68-69 -0.4781161481462064 2215 70-71 -0.4204429549459725 2215 72-73 -0.335763342381167 2215 74-75 -0.31054950653670943 2215 76-77 -0.3341061547870616 2215 78-79 -0.2164907440138606 2215 80-81 -0.29078918048984903 2215 82-83 -0.2732085581872674 2215 84-85 -0.26276417687594744 2215 86-87 -0.25239615115451386 2215 88-89 -0.1857636386142616 2215 90-91 -0.19225933422787023 2215 92-93 -0.11760020724719666 2215 94-95 -0.1740970259229293 2215 96-97 -0.2915882692278764 2215 98-99 -0.24766571713495722 2215 100 -0.3285322834856004 2216 1 -0.5538773254100668 2216 2 -0.5698494677919435 2216 3 -0.6040661670050049 2216 4 -0.48232763970584713 2216 5 -0.7379834926746796 2216 6 -0.6589512782992024 2216 7 -0.6592365825187265 2216 8 -0.7652290079163713 2216 9 -0.8945059835389628 2216 10-11 -1.1266569011052212 2216 12-13 -0.9229110273892829 2216 14-15 -1.2162218200937076 2216 16-17 -1.0894595207779147 2216 18-19 -1.0312597681892868 2216 20-21 -0.9731621460633733 2216 22-23 -0.8940166582023963 2216 24-25 -0.9243166808892127 2216 26-27 -0.9633834728544244 2216 28-29 -4.261635385203352 2216 30-31 -1.9313829242314924 2216 32-33 -1.3983779564112027 2216 34-35 -1.1491826129177483 2216 36-37 -1.2727137169987017 2216 38-39 -1.2965642606800927 2216 40-41 -1.1780818800159807 2216 42-43 -1.2841813900146732 2216 44-45 -1.1162572444362837 2216 46-47 -1.1085795940982237 2216 48-49 -1.0570162357288453 2216 50-51 -0.9287274652007511 2216 52-53 -1.0638360531538034 2216 54-55 -1.3079475225185817 2216 56-57 -1.0849859679602787 2216 58-59 -1.1561650218793424 2216 60-61 -1.0975227789120048 2216 62-63 -1.1932037925257575 2216 64-65 -1.1283899481194588 2216 66-67 -1.259352420820587 2216 68-69 -1.2902320698389644 2216 70-71 -1.3099159664679796 2216 72-73 -1.3093766629878054 2216 74-75 -1.2245102550186147 2216 76-77 -1.2011296039931345 2216 78-79 -1.2193178105380298 2216 80-81 -1.0875884712757795 2216 82-83 -1.2132530472295322 2216 84-85 -1.2264096133498903 2216 86-87 -1.3163162449187062 2216 88-89 -1.2748374452827278 2216 90-91 -1.340903315833991 2216 92-93 -1.3073573938863419 2216 94-95 -1.3015942997453713 2216 96-97 -1.3058424387158993 2216 98-99 -1.5499214421078307 2216 100 -1.508279435615826 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 111729.0 3 22200.0 4 16152.0 5 14159.0 6 9052.0 7 7506.0 8 7832.0 9 7934.0 10 8177.0 11 8227.0 12 9146.0 13 9710.0 14 10040.0 15 10091.0 16 10243.0 17 10327.0 18 9956.0 19 9277.0 20 9015.0 21 9795.0 22 12220.0 23 20511.0 24 32018.0 25 37135.0 26 40484.0 27 31443.0 28 30746.0 29 35066.0 30 40569.0 31 45486.0 32 56078.0 33 73375.0 34 99577.0 35 158984.0 36 235262.0 37 396578.0 38 479100.0 39 185692.0 40 462.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.20491074717653 21.991856064930886 12.3740247161665 21.429208471726085 2 16.376750343681916 24.199256673417523 37.410686859783375 22.013306123117182 3 17.29789858384791 30.94885334806167 28.393868726221367 23.35937934186905 4 12.091420201193374 21.51157831247442 36.330008831829055 30.066992654503157 5 11.500220583927016 41.04674828271966 31.862314270056768 15.590716863296558 6 26.39480512989976 41.59222148165988 18.190920609945717 13.822052778494646 7 24.656078273401132 36.99279865753017 20.907287293281833 17.44383577578686 8 23.938139604215475 37.7738822193367 20.210265375704065 18.077712800743765 9 24.307455028358362 21.91596102852638 20.465925809446468 33.310658133668795 10-11 21.866036417401705 31.5553003233675 26.723441946787823 19.85522131244297 12-13 22.88514089582769 30.012411774513254 26.304065642390107 20.79838168726895 14-15 21.069264625040283 31.657997135909866 24.485170320305915 22.787567918743935 16-17 19.83064567547867 34.31338929221946 24.73167220109356 21.124292831208308 18-19 19.707556877283704 32.608127637236464 27.075889727031687 20.608425758448153 20-21 22.098182696249182 31.976593277611045 25.915194789277248 20.01002923686253 22-23 21.367728488595763 32.43549992913145 26.045332606122955 20.151438976149826 24-25 20.755179898746707 32.07286204578348 26.52086187001297 20.651096185456844 26-27 20.559045144947206 31.736436913824374 26.754638784416613 20.949879156811807 28-29 20.63628878434326 31.840075348683705 26.725150079084237 20.7984857878888 30-31 21.17661402246198 32.72082272554655 25.896283040127965 20.206280211863504 32-33 20.19131331238313 32.00685329709375 26.971653034817706 20.83018035570542 34-35 20.46069669723747 31.121453836737395 27.0922209774285 21.32562848859664 36-37 21.23065801417468 32.061351527519825 26.176696259361076 20.531294198944416 38-39 21.003392000634154 31.85387363801384 26.574764351057738 20.56797001029426 40-41 20.51593008117332 31.492702500338186 26.953962724549225 21.037404693939273 42-43 20.340164481761825 31.70463504262826 26.90620920804918 21.048991267560734 44-45 20.9783835084483 31.74376420848022 26.62957293891145 20.64827934416003 46-47 20.543151381756246 32.454044673981436 26.358454666336662 20.644349277925656 48-49 20.392459690491037 31.63828226726246 26.924651832035007 21.04460621021149 50-51 20.49294720392204 31.279627182424928 26.88310247636772 21.34432313728531 52-53 20.49349964545087 31.4953787989099 26.654641577971944 21.35647997766728 54-55 20.824515227670297 32.122029873534096 26.35346478584416 20.699990112951454 56-57 20.3100145027837 31.79517235868079 26.28902563824203 21.60578750029348 58-59 20.29112436435822 31.025220611422338 27.03594919346084 21.6477058307586 60-61 20.96908784256792 31.85770197587081 26.184563953550988 20.98864622801028 62-63 20.621972013499555 31.73948050672436 26.44066029599416 21.197887183781923 64-65 20.630975682940576 31.233886509762815 26.87057575712537 21.264562050171236 66-67 20.440962527727365 31.345543352907445 26.3517748742818 21.86171924508339 68-69 21.281516039682785 31.705796661595425 26.313958570318647 20.698728728403147 70-71 21.286514386473872 31.285025948577594 26.470414011908794 20.958045653039743 72-73 20.824436491465388 31.40509610527801 27.007699407547335 20.762767995709268 74-75 20.795617930212998 31.315471760700735 26.783872242762875 21.10503806632339 76-77 20.929265767260947 31.440435605713844 26.18741393463545 21.44288469238976 78-79 21.229097451026345 31.568492466121672 26.503113842209018 20.699296240642965 80-81 20.676898951964407 31.343189384156577 26.81543006508354 21.164481598795476 82-83 20.590350616260682 31.49034904386489 26.82539535522898 21.093904984645448 84-85 20.766803717961533 31.23537439817479 26.61844454247499 21.379377341388686 86-87 21.23365391864385 31.444227848919237 26.339016273384242 20.983101959052675 88-89 20.784454367066658 31.26345423567448 26.62007629362059 21.332015103638273 90-91 20.623826620244124 31.03398288707208 26.55840340225203 21.78378709043177 92-93 20.85846054629973 31.500688308110448 26.299013277852247 21.341837867737574 94-95 20.823851367287833 31.11258059895511 26.618747960457373 21.44482007329968 96-97 20.770692805884945 31.302658752758134 26.550414980480884 21.376233460876037 98-99 20.6162748319693 31.314753530561728 26.5744181498142 21.494553487654773 100 20.811231710022682 31.61115502835714 26.268079964330106 21.309533297290077 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 36920.0 1 28006.0 2 17207.0 3 12566.5 4 7973.5 5 5261.5 6 4011.0 7 3891.0 8 4241.5 9 4182.0 10 3321.5 11 2388.0 12 2131.5 13 2055.0 14 2050.0 15 2129.0 16 2206.0 17 2348.0 18 2666.5 19 3075.0 20 3712.5 21 4667.0 22 6029.0 23 8232.0 24 10839.5 25 13884.5 26 17969.0 27 23130.5 28 29689.5 29 35810.5 30 40451.5 31 44989.5 32 49804.0 33 54850.0 34 58969.0 35 62613.0 36 65792.0 37 67924.5 38 69662.5 39 70446.5 40 71308.5 41 70794.5 42 68788.0 43 68250.5 44 67484.5 45 66428.5 46 67033.0 47 67740.0 48 68881.5 49 71331.5 50 73462.0 51 71095.5 52 66048.5 53 61728.5 54 59088.0 55 58603.0 56 54888.0 57 49372.0 58 45988.5 59 42699.5 60 37361.5 61 31343.5 62 25924.0 63 20167.5 64 15184.5 65 11748.0 66 9110.0 67 6896.5 68 4993.5 69 3732.5 70 2880.5 71 2220.5 72 1790.5 73 1448.0 74 1232.5 75 1009.0 76 760.5 77 632.5 78 526.5 79 385.5 80 255.0 81 176.5 82 144.0 83 113.0 84 94.5 85 88.0 86 69.0 87 65.5 88 53.5 89 36.0 90 33.0 91 28.5 92 24.0 93 16.5 94 13.0 95 13.5 96 15.0 97 15.5 98 18.5 99 23.0 100 36.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015637425399142053 2 0.007194077249743038 3 0.008831052911361213 4 0.008787974604476525 5 0.010424950266094702 6 0.01270810053098321 7 0.011157281483134412 8 0.008271034921860258 9 0.007280233863512416 10-11 0.006720215874011461 12-13 0.0073663904772817935 14-15 0.008529504763168392 16-17 0.005427866667470795 18-19 0.006483285186145672 20-21 0.006052502117298784 22-23 0.006677137567126772 24-25 0.0062678936517222275 26-27 0.007064842329088971 28-29 0.01770518412960712 30-31 0.006138658731068161 32-33 0.005837110582875339 34-35 0.005621719048451894 36-37 0.007452547091051171 38-39 0.006784833334338494 40-41 0.006375589418933949 42-43 0.005341710053701417 44-45 0.005040161905508596 46-47 0.006870989948107871 48-49 0.004157056614372474 50-51 0.004803231217642807 52-53 0.004135517460930129 54-55 0.005837110582875339 56-57 0.0032524121697940084 58-59 0.0033601079370057306 60-61 0.004264752381584196 62-63 0.0039632042333913745 64-65 0.005320170900259073 66-67 0.003920125926506686 68-69 0.004113978307487785 70-71 0.005535562434682517 72-73 0.0030800989422552524 74-75 0.0032954904766786973 76-77 0.003467803704217453 78-79 0.004566300529777018 80-81 0.004587839683219363 82-83 0.002390846032100231 84-85 0.0035324211645444857 86-87 0.0034031862438904195 88-89 0.0025416201061966423 90-91 0.0029293248681588418 92-93 0.00355396031798683 94-95 0.002649315873408364 96-97 0.0017231322753875542 98-99 0.00224007195800382 100 0.0029724031750435306 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2321354.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.07135143135962 #Duplication Level Percentage of deduplicated Percentage of total 1 82.28846261448265 56.01486857376747 2 11.967446413571361 16.29280501108361 3 3.060684270271987 6.250347437463567 4 1.0402717010523415 2.8325080218572882 5 0.4693139655484821 1.5973417940247863 6 0.27618173009467356 1.128003816491726 7 0.16999977002535915 0.8100479862052578 8 0.12111148373050146 0.6595377897113887 9 0.08236540871807321 0.5046052214371981 >10 0.4614194350333243 5.707933962277443 >50 0.036989268960465956 1.7338064262724686 >100 0.023531278550765936 2.864543248617185 >500 0.0014606051165076566 0.6903146264007215 >1k 6.350457028289803E-4 0.7942789746058132 >5k 6.350457028289803E-5 0.26183453516857574 >10k+ 6.350457028289803E-5 1.857222574615737 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42963 1.850773298686887 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6057 0.26092530480056036 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3722 0.16033745822481188 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.307830688468885E-5 0.0 10-11 2.1539153442344424E-5 0.0 0.0 4.307830688468885E-5 0.0 12-13 4.307830688468885E-5 4.307830688468885E-5 0.0 6.461746032703327E-5 0.0 14-15 4.307830688468885E-5 4.307830688468885E-5 0.0 8.61566137693777E-5 0.0 16-17 4.307830688468885E-5 4.307830688468885E-5 0.0 1.2923492065406654E-4 0.0 18-19 4.307830688468885E-5 4.307830688468885E-5 0.0 1.5077407409641098E-4 0.0 20-21 4.307830688468885E-5 4.307830688468885E-5 0.0 2.800089947504775E-4 0.0 22-23 4.307830688468885E-5 4.307830688468885E-5 0.0 0.0012061925927712878 0.0 24-25 4.307830688468885E-5 4.307830688468885E-5 0.0 0.0028647074078318084 0.0 26-27 4.307830688468885E-5 4.307830688468885E-5 0.0 0.004544761376334674 0.0 28-29 4.307830688468885E-5 4.307830688468885E-5 0.0 0.009218757673323414 0.0 30-31 4.307830688468885E-5 4.307830688468885E-5 0.0 0.01908368994991716 0.0 32-33 4.307830688468885E-5 4.307830688468885E-5 0.0 0.036142699476253946 0.0 34-35 4.307830688468885E-5 4.307830688468885E-5 0.0 0.05992192487660219 0.0 36-37 4.307830688468885E-5 4.307830688468885E-5 0.0 0.09384609154829465 0.0 38-39 4.307830688468885E-5 4.307830688468885E-5 0.0 0.14418309314305358 0.0 40-41 4.307830688468885E-5 4.307830688468885E-5 0.0 0.2074651259566615 0.0 42-43 4.307830688468885E-5 4.307830688468885E-5 0.0 0.270596384696173 0.0 44-45 4.307830688468885E-5 4.307830688468885E-5 0.0 0.3403617026959266 0.0 46-47 4.307830688468885E-5 4.307830688468885E-5 0.0 0.42382592228501126 0.0 48-49 4.307830688468885E-5 4.307830688468885E-5 0.0 0.5166596736215157 0.0 50-51 4.307830688468885E-5 4.307830688468885E-5 0.0 0.6178075381867651 0.0 52-53 6.461746032703327E-5 4.307830688468885E-5 0.0 0.7162414694182792 0.0 54-55 8.61566137693777E-5 4.307830688468885E-5 0.0 0.8223002609683832 0.0 56-57 8.61566137693777E-5 4.307830688468885E-5 0.0 0.950178214955582 0.0 58-59 8.61566137693777E-5 4.307830688468885E-5 0.0 1.0899673207963971 0.0 60-61 8.61566137693777E-5 4.307830688468885E-5 0.0 1.2360889377492619 0.0 62-63 1.2923492065406654E-4 4.307830688468885E-5 0.0 1.3816074584057407 0.0 64-65 1.2923492065406654E-4 4.307830688468885E-5 0.0 1.544529615043634 0.0 66-67 1.2923492065406654E-4 4.307830688468885E-5 0.0 1.7350649663946127 0.0 68-69 1.2923492065406654E-4 4.307830688468885E-5 0.0 1.9389115145729605 0.0 70-71 1.2923492065406654E-4 4.307830688468885E-5 0.0 2.158309331536681 0.0 72-73 1.2923492065406654E-4 8.61566137693777E-5 0.0 2.4144098659661557 0.0 74-75 1.2923492065406654E-4 8.61566137693777E-5 0.0 2.664500976585217 0.0 76-77 1.2923492065406654E-4 8.61566137693777E-5 0.0 2.92057997186125 0.0 78-79 1.2923492065406654E-4 8.61566137693777E-5 0.0 3.2115523957138805 0.0 80-81 1.2923492065406654E-4 8.61566137693777E-5 0.0 3.532141155549735 0.0 82-83 1.2923492065406654E-4 8.61566137693777E-5 0.0 3.8724167016318924 0.0 84-85 1.2923492065406654E-4 8.61566137693777E-5 0.0 4.204162742950881 0.0 86-87 1.2923492065406654E-4 8.61566137693777E-5 0.0 4.560118792739065 0.0 88 1.2923492065406654E-4 8.61566137693777E-5 0.0 4.84781726526846 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2520 0.0 50.175087 2 GTACCTG 2975 0.0 43.45485 1 GGTATCA 4470 0.0 37.229588 1 TCTGTCG 670 0.0 33.671726 8 GTATCAA 7055 0.0 32.45073 1 ACCTGGG 3855 0.0 32.18963 3 GTCTCGC 815 0.0 29.417454 1 TCGCTCT 760 0.0 27.831417 4 GTACATG 12135 0.0 27.311268 1 TATAACG 175 5.5551936E-9 26.859526 2 TACATGG 12125 0.0 26.787529 2 ATCAACG 8000 0.0 26.674864 3 TATCAAC 8475 0.0 26.566368 2 TCAACGC 8060 0.0 26.53461 4 CGCTCTG 710 0.0 25.156618 5 CAACGCA 8805 0.0 24.288969 5 TCTCGCT 895 0.0 24.158567 2 CTGTCGC 920 0.0 24.01093 9 ACATGGG 13105 0.0 23.421383 3 CATGGGT 5515 0.0 23.352932 4 >>END_MODULE