##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431432_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2321354
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.205076433839906	33.0	31.0	34.0	30.0	34.0
2	32.30125866197056	33.0	31.0	34.0	30.0	34.0
3	32.431684697809985	34.0	31.0	34.0	30.0	34.0
4	36.00771661711225	37.0	35.0	37.0	35.0	37.0
5	35.93250189329159	37.0	35.0	37.0	35.0	37.0
6	35.98503761166974	37.0	35.0	37.0	35.0	37.0
7	35.92879543576723	37.0	35.0	37.0	35.0	37.0
8	35.909769470748536	37.0	35.0	37.0	35.0	37.0
9	37.58243464805454	39.0	37.0	39.0	35.0	39.0
10-11	37.51959804493412	39.0	37.0	39.0	34.5	39.0
12-13	37.4965029030471	39.0	37.0	39.0	34.5	39.0
14-15	38.78027414172935	40.0	38.0	41.0	34.0	41.0
16-17	38.837236371531446	40.0	38.0	41.0	35.0	41.0
18-19	38.93595311184765	40.0	38.5	41.0	35.0	41.0
20-21	38.912456695532	40.0	38.5	41.0	35.0	41.0
22-23	38.760508306789916	40.0	38.0	41.0	34.5	41.0
24-25	38.66204249761131	40.0	38.0	41.0	34.0	41.0
26-27	38.46861142247154	40.0	38.0	41.0	34.0	41.0
28-29	38.32404213230727	40.0	38.0	41.0	34.0	41.0
30-31	38.02958984282449	40.0	37.0	41.0	33.5	41.0
32-33	37.66892813418376	40.0	36.0	41.0	33.0	41.0
34-35	37.39791496686847	40.0	36.0	41.0	33.0	41.0
36-37	37.17617907479858	40.0	35.0	41.0	32.5	41.0
38-39	37.035478862767164	40.0	35.0	41.0	32.0	41.0
40-41	36.865948063070086	40.0	35.0	41.0	31.0	41.0
42-43	36.66852427505671	40.0	35.0	41.0	31.0	41.0
44-45	36.352762654898825	40.0	35.0	41.0	30.0	41.0
46-47	35.88228615713071	40.0	35.0	41.0	28.0	41.0
48-49	35.504913080900195	40.0	35.0	41.0	26.0	41.0
50-51	35.33019091444045	40.0	35.0	41.0	24.5	41.0
52-53	35.1829262146144	39.0	35.0	41.0	24.0	41.0
54-55	35.00981862309669	39.0	35.0	41.0	24.0	41.0
56-57	34.78014512220024	39.0	35.0	41.0	23.0	41.0
58-59	34.60415882282496	39.0	35.0	41.0	23.0	41.0
60-61	34.37849332760105	38.0	35.0	41.0	23.0	41.0
62-63	34.1265539422251	37.5	34.0	40.5	22.0	41.0
64-65	33.86734186168934	37.0	34.0	40.0	22.0	41.0
66-67	33.60006638367091	37.0	34.0	40.0	21.5	41.0
68-69	33.30081667854192	36.0	34.0	39.5	20.0	41.0
70-71	32.964124601417964	36.0	34.0	39.0	19.5	41.0
72-73	32.642493346555504	35.0	33.0	39.0	18.0	41.0
74-75	32.345192719421505	35.0	33.0	38.5	17.0	40.5
76-77	32.015710658520845	35.0	33.0	37.0	13.0	39.5
78-79	31.714833024174684	35.0	33.0	37.0	11.0	39.0
80-81	31.428267511116356	35.0	33.0	36.0	7.5	39.0
82-83	31.188470608101994	35.0	33.0	36.0	7.0	38.0
84-85	30.96852871212232	35.0	33.0	36.0	2.0	37.0
86-87	30.7322635840979	35.0	33.0	35.0	2.0	37.0
88-89	30.579998785191748	35.0	33.0	35.0	2.0	36.5
90-91	30.392116195978726	35.0	32.5	35.0	2.0	36.0
92-93	30.1947283783516	35.0	32.0	35.0	2.0	36.0
94-95	30.024068280839543	35.0	32.0	35.0	2.0	36.0
96-97	29.850113554416946	35.0	32.0	35.0	2.0	35.5
98-99	29.68400898785795	35.0	32.0	35.0	2.0	35.0
100	29.564911254379986	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15438798254817954
1101	2	0.27229888528694346
1101	3	0.28414090303026995
1101	4	0.1566179707451667
1101	5	0.13231388245842624
1101	6	0.20637363147938714
1101	7	0.19766542626901895
1101	8	0.24699401329242932
1101	9	0.38017946289061655
1101	10-11	0.2628761364271881
1101	12-13	0.389634998655346
1101	14-15	0.4712820480750253
1101	16-17	0.42913175432682493
1101	18-19	0.3902019119958666
1101	20-21	0.3192861025137148
1101	22-23	0.2729383786411148
1101	24-25	0.30793642065817295
1101	26-27	0.2665157368990023
1101	28-29	0.2783865491034305
1101	30-31	0.3118867292804879
1101	32-33	0.3229436421383767
1101	34-35	0.3162789282177272
1101	36-37	0.3341668382278513
1101	38-39	0.35555543034143966
1101	40-41	0.472909959903113
1101	42-43	0.490777595014535
1101	44-45	0.525337989650545
1101	46-47	0.5648452254021521
1101	48-49	0.6025488693223764
1101	50-51	0.5174591501241963
1101	52-53	0.4583282280538512
1101	54-55	0.43730496978253797
1101	56-57	0.3724623005321561
1101	58-59	0.3769273263441022
1101	60-61	0.4746971190574456
1101	62-63	0.3608028524060529
1101	64-65	0.3249821981269676
1101	66-67	0.26020827132997937
1101	68-69	0.3119436282833732
1101	70-71	0.33099081013850906
1101	72-73	0.35066953069434703
1101	74-75	0.3081613154086327
1101	76-77	0.2856251515082562
1101	78-79	0.23163256372224694
1101	80-81	0.20804801290723063
1101	82-83	0.21593153390164943
1101	84-85	0.21539066720611544
1101	86-87	0.3034511476143358
1101	88-89	0.2537793974616491
1101	90-91	0.2622487715512136
1101	92-93	0.41666486987933027
1101	94-95	0.4043856239966175
1101	96-97	0.4337745354733791
1101	98-99	0.5000037128998862
1101	100	0.48799670790121397
1102	1	0.17564385015574402
1102	2	0.2466060823841758
1102	3	0.23818757083083142
1102	4	0.19440119815205747
1102	5	0.12545440768234073
1102	6	0.19822232848579802
1102	7	0.25039463755379643
1102	8	0.19803210400156246
1102	9	0.3214383040750022
1102	10-11	0.3192296114046016
1102	12-13	0.3725538535621524
1102	14-15	0.4540546828376506
1102	16-17	0.4166052727161471
1102	18-19	0.4217539339702512
1102	20-21	0.41250339692905413
1102	22-23	0.3896036857930838
1102	24-25	0.34799972776155386
1102	26-27	0.372645962208189
1102	28-29	0.4507423508757782
1102	30-31	0.391784540056868
1102	32-33	0.41277632348317894
1102	34-35	0.44426957128553113
1102	36-37	0.5802376961336861
1102	38-39	0.6023735874411784
1102	40-41	0.6244981623238601
1102	42-43	0.5484085799163978
1102	44-45	0.4706947552320244
1102	46-47	0.417619763485348
1102	48-49	0.4337764011726222
1102	50-51	0.41529957549609264
1102	52-53	0.3517843631676243
1102	54-55	0.25693179167686253
1102	56-57	0.24587485018336963
1102	58-59	0.23040288226990668
1102	60-61	0.23399938140559584
1102	62-63	0.30280219521086593
1102	64-65	0.22979592147324723
1102	66-67	0.1729139774734776
1102	68-69	0.1602705285088888
1102	70-71	0.25065000005287885
1102	72-73	0.182668225560235
1102	74-75	0.24258555804325255
1102	76-77	0.2570919753001135
1102	78-79	0.20360703608591635
1102	80-81	0.09979376283288843
1102	82-83	0.04141468228312917
1102	84-85	-0.013819749800429548
1102	86-87	0.055700565915600464
1102	88-89	0.01979809628459961
1102	90-91	0.0642097124048071
1102	92-93	0.03108220502226189
1102	94-95	0.09557620018787105
1102	96-97	0.12281563215627855
1102	98-99	0.16643000579836098
1102	100	0.18324124120585594
1103	1	0.10147582201534533
1103	2	0.15478761422906473
1103	3	0.16771629320575698
1103	4	0.13347109443834881
1103	5	0.15048569703006365
1103	6	0.1476454677232013
1103	7	0.19403075811276693
1103	8	0.1879132951192446
1103	9	0.2697765130327099
1103	10-11	0.28618040309873294
1103	12-13	0.29599536268685966
1103	14-15	0.36949073587054215
1103	16-17	0.3586412839795585
1103	18-19	0.3474480117672414
1103	20-21	0.29239696027403284
1103	22-23	0.3073849292548019
1103	24-25	0.3691868365502913
1103	26-27	0.35472015893869013
1103	28-29	0.35681388168587347
1103	30-31	0.33803871228461446
1103	32-33	0.2892010557015041
1103	34-35	0.2975366928090537
1103	36-37	0.29780813776479675
1103	38-39	0.1818420631873039
1103	40-41	0.16505440648424496
1103	42-43	0.21473015736782486
1103	44-45	0.24737019877788669
1103	46-47	0.276941619274119
1103	48-49	0.2632047502658139
1103	50-51	0.24910361807226877
1103	52-53	0.25293990691492496
1103	54-55	0.3159398287149884
1103	56-57	0.40797090224944554
1103	58-59	0.3573500635094433
1103	60-61	0.36742862216022587
1103	62-63	0.2672971413297418
1103	64-65	0.25419774165599307
1103	66-67	0.21395381861347573
1103	68-69	0.2613196374894784
1103	70-71	0.33260878308797004
1103	72-73	0.3025035004570782
1103	74-75	0.23501752967624867
1103	76-77	0.25542298978498224
1103	78-79	0.24575267336514628
1103	80-81	0.13605060135870772
1103	82-83	0.12666677774415547
1103	84-85	0.09323037093831132
1103	86-87	0.1752525938505478
1103	88-89	0.15691965001881414
1103	90-91	0.17686344941415655
1103	92-93	0.22658176013570497
1103	94-95	0.17530328041020482
1103	96-97	0.15024828924692812
1103	98-99	0.22206778165257646
1103	100	0.29420777047886304
1104	1	0.05001042833161051
1104	2	0.10763365558744198
1104	3	0.1036545608984838
1104	4	0.14216628208607318
1104	5	0.13151523748283722
1104	6	0.12552901246125003
1104	7	0.09869876598557425
1104	8	0.0952054493540686
1104	9	0.18937827947096508
1104	10-11	0.19837943120882784
1104	12-13	0.08465745868969066
1104	14-15	0.2087978391269445
1104	16-17	0.22212516624801282
1104	18-19	0.26351221377382217
1104	20-21	0.2308191000598896
1104	22-23	0.18686025988052535
1104	24-25	0.1455763876435583
1104	26-27	0.21090432815161364
1104	28-29	0.22610406481283718
1104	30-31	0.249124813066949
1104	32-33	0.20593955539187903
1104	34-35	0.21895984431398574
1104	36-37	0.12079818192575686
1104	38-39	0.17102186572765277
1104	40-41	0.2724547099999768
1104	42-43	0.2714205858657408
1104	44-45	0.2155802700343017
1104	46-47	0.29250970099078444
1104	48-49	0.14176411898800012
1104	50-51	0.08099744426009892
1104	52-53	0.14478826388896238
1104	54-55	0.17150148543404953
1104	56-57	0.11529936062795798
1104	58-59	0.14250275895583542
1104	60-61	0.08321987079019522
1104	62-63	0.1814465632253217
1104	64-65	0.10495754849905126
1104	66-67	0.1702543863971968
1104	68-69	0.14300664998187784
1104	70-71	0.2202990618630878
1104	72-73	0.12201194453197672
1104	74-75	0.07982567372412319
1104	76-77	0.05708316846387618
1104	78-79	0.1582262538331527
1104	80-81	0.14993230290739135
1104	82-83	0.16118278790785823
1104	84-85	0.1761457281361949
1104	86-87	0.10360805611604462
1104	88-89	0.08703980619567986
1104	90-91	0.0756933585048003
1104	92-93	0.09080844190063075
1104	94-95	-0.03854466082868413
1104	96-97	0.012254022321716462
1104	98-99	-0.006391941462005946
1104	100	0.08602258518600081
1105	1	0.12608490660820593
1105	2	0.12693214112714912
1105	3	0.14591980319786302
1105	4	0.10752136301492499
1105	5	0.03069650119992673
1105	6	0.13080522982166798
1105	7	0.11283813071330684
1105	8	0.1690210777936727
1105	9	0.18776441760591212
1105	10-11	0.16063467245381702
1105	12-13	0.1640594450508175
1105	14-15	0.3029841346897655
1105	16-17	0.22635090751440146
1105	18-19	0.23066554448335808
1105	20-21	0.2171324622549946
1105	22-23	0.1926802454252794
1105	24-25	0.25669875023084643
1105	26-27	0.30518845066001177
1105	28-29	0.3098074354187119
1105	30-31	0.26523959482717174
1105	32-33	0.24169149792444244
1105	34-35	0.3209801686557654
1105	36-37	0.3742532260727174
1105	38-39	0.36997468678011103
1105	40-41	0.4473043462003119
1105	42-43	0.4466840617993597
1105	44-45	0.4201555958254417
1105	46-47	0.3329063572840525
1105	48-49	0.3407969328163176
1105	50-51	0.31502137754185355
1105	52-53	0.3869829002754628
1105	54-55	0.31636883748961253
1105	56-57	0.3296625572349825
1105	58-59	0.29937994538154555
1105	60-61	0.3033123596642824
1105	62-63	0.26111101636703893
1105	64-65	0.3217662882513608
1105	66-67	0.26995670365612057
1105	68-69	0.29632457111274135
1105	70-71	0.19410871368094007
1105	72-73	0.21147949278081057
1105	74-75	0.26447388124216786
1105	76-77	0.26406997848594926
1105	78-79	0.1829715122928981
1105	80-81	0.25493258651419026
1105	82-83	0.18400069459739754
1105	84-85	0.27539166464237397
1105	86-87	0.22106167944515676
1105	88-89	0.24321136847242641
1105	90-91	0.3345683808192632
1105	92-93	0.34285458664031765
1105	94-95	0.29145446573198086
1105	96-97	0.25435739995686646
1105	98-99	0.410657811869676
1105	100	0.4371803446853484
1106	1	0.05921185410084462
1106	2	0.18798215243270988
1106	3	0.0974782047210283
1106	4	0.1600653002144199
1106	5	0.09278455282116482
1106	6	0.16048732636773622
1106	7	0.19682178824114516
1106	8	0.17982739152324
1106	9	0.2462817972696243
1106	10-11	0.18069456814392737
1106	12-13	0.1789975921072653
1106	14-15	0.2680315433405198
1106	16-17	0.2139629615475016
1106	18-19	0.2192786750907203
1106	20-21	0.26118152905419834
1106	22-23	0.17969175536568116
1106	24-25	0.22602351306876045
1106	26-27	0.22378673805474847
1106	28-29	0.17122243290297234
1106	30-31	0.222247835322527
1106	32-33	0.29310963244632404
1106	34-35	0.27257413345711257
1106	36-37	0.21570249840900146
1106	38-39	0.23983961252670838
1106	40-41	0.3555613585318582
1106	42-43	0.3815009440185335
1106	44-45	0.2969336748584155
1106	46-47	0.23589482697327924
1106	48-49	0.16982791581588685
1106	50-51	0.28928565904141834
1106	52-53	0.22967281327360212
1106	54-55	0.2086982542790139
1106	56-57	0.2054896793017349
1106	58-59	0.17454290837468278
1106	60-61	0.2091649463962355
1106	62-63	0.1640393085759797
1106	64-65	0.20382907891113433
1106	66-67	0.2343788266972524
1106	68-69	0.22983049244099618
1106	70-71	0.14488009731143592
1106	72-73	0.2433685496121143
1106	74-75	0.19831222161347029
1106	76-77	0.22923935475188273
1106	78-79	0.1899760351949702
1106	80-81	0.18209763475462992
1106	82-83	0.14291339532197256
1106	84-85	0.1907008580638312
1106	86-87	0.20804365234305422
1106	88-89	0.16608268134983106
1106	90-91	0.2276894837120622
1106	92-93	0.20403405167354904
1106	94-95	0.1846418147790203
1106	96-97	0.25117813625082874
1106	98-99	0.3276653003587171
1106	100	0.34384286598087854
1107	1	0.07635019037025614
1107	2	0.14440025173347948
1107	3	0.0798177274219185
1107	4	0.11551009289994596
1107	5	0.11458112826426259
1107	6	0.13779421029095573
1107	7	0.06261301388945384
1107	8	0.08206558312848244
1107	9	0.052405174761048556
1107	10-11	0.09467696226311517
1107	12-13	0.003542902566096018
1107	14-15	0.18884826058891235
1107	16-17	0.11715088400856644
1107	18-19	0.0687697033975212
1107	20-21	0.008682663999536544
1107	22-23	0.01084630249271612
1107	24-25	0.01480125305179314
1107	26-27	0.05478907677008493
1107	28-29	0.07750192328273897
1107	30-31	0.06572017905411798
1107	32-33	0.124323904830554
1107	34-35	0.10725478697386137
1107	36-37	0.1432687487746378
1107	38-39	0.14950185213450595
1107	40-41	0.10240386120874234
1107	42-43	0.16922538524821817
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2216	72-73	-1.1552269448650492
2216	74-75	-1.1618644765272705
2216	76-77	-1.0405704693502855
2216	78-79	-0.9610177634786403
2216	80-81	-1.0042384833680345
2216	82-83	-0.9494160398728937
2216	84-85	-1.0514618934666693
2216	86-87	-1.0602004005877745
2216	88-89	-1.138829086438971
2216	90-91	-1.1219797340256044
2216	92-93	-1.1009271510834786
2216	94-95	-1.3247071309388332
2216	96-97	-1.5033432156893163
2216	98-99	-1.5025624475314459
2216	100	-1.4708123357392893
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	181.0
3	511.0
4	403.0
5	387.0
6	819.0
7	1734.0
8	2891.0
9	4359.0
10	5936.0
11	7182.0
12	10355.0
13	14444.0
14	13309.0
15	15538.0
16	19762.0
17	28014.0
18	31074.0
19	16888.0
20	10872.0
21	9897.0
22	9739.0
23	10241.0
24	11400.0
25	13547.0
26	15968.0
27	19800.0
28	24515.0
29	30175.0
30	37145.0
31	46152.0
32	57948.0
33	73618.0
34	102051.0
35	166183.0
36	227316.0
37	419742.0
38	588213.0
39	271397.0
40	1648.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.1215047683407	20.330310166254076	13.75246519421593	22.795719871189295
2	14.760695697424865	22.05997017258031	39.62234971486468	23.556984415130135
3	16.18667954424737	29.024139863622285	31.106379184154715	23.682801407975628
4	10.845927701287168	18.656964764507592	37.57019941202335	32.92690812218189
5	10.506376502041782	37.706337557625034	34.77383159210721	17.01345434822598
6	26.359615982741108	38.808902045961105	19.454895720342524	15.376586250955263
7	23.564221570686765	34.027985391284574	24.051523378166365	18.356269659862303
8	25.056109494717305	32.35788251167207	22.75641715998508	19.82959083362555
9	25.028237830162915	15.245628198025807	24.207466849088938	35.51866712272234
10-11	22.45958996709293	26.626741321504365	29.77217090994631	21.141497801456392
12-13	24.79046711531287	25.056281807944845	30.25322721136027	19.900023865382014
14-15	21.92754745721678	25.964027890619008	30.352221160581283	21.75620349158293
16-17	18.660574819695746	28.89509743020668	30.552470670134756	21.891857079962815
18-19	18.349614061448623	25.37624593233087	36.222911283673234	20.051228722547272
20-21	20.280965333163316	24.86955888675316	35.96717691485228	18.88229886523124
22-23	19.597484916130846	25.365325581535604	36.12217266302339	18.915016839310162
24-25	19.154118426780375	25.002288547376594	36.48377457603718	19.359818449805854
26-27	18.972806387995973	24.850130570348167	36.535573635042304	19.641489406613555
28-29	19.03857768480474	24.976565279911362	36.51790645238832	19.466950582895585
30-31	19.72368089733811	25.74761482475831	35.713924150425704	18.814780127477878
32-33	18.664905334119368	25.11908487674298	36.60075167515778	19.61525811397987
34-35	18.930500219811275	24.56938637437188	36.3217399489383	20.178373456878543
36-37	19.9334325182493	25.511453186823413	35.09373276119993	19.46138153372736
38-39	19.7129219581962	25.32063653438561	35.42057967018765	19.54586183723054
40-41	19.195375065156615	25.25420771712768	35.550934162742905	19.999483054972796
42-43	19.205190172790367	25.375994589262096	35.257203906414915	20.161611331532626
44-45	19.935929634170403	25.55814408315147	34.570427431576576	19.935498851101556
46-47	19.712972065296714	26.442161473837118	33.648962539772256	20.19590392109392
48-49	19.865090765521703	25.887952144850914	33.53670737809242	20.71024971153496
50-51	19.90058227734133	25.749030776285785	33.21595741037468	21.134429535998215
52-53	20.034445851926346	26.14722758494127	32.70837403387592	21.109952529256464
54-55	20.334201660001646	26.88507803524111	32.3028505727122	20.477869732045047
56-57	19.80530768339321	26.25819564957968	32.48555381459856	21.450942852428547
58-59	19.699832388142617	25.46845047673844	33.230940963618465	21.60077617150047
60-61	20.378749205733797	26.317835718824377	32.29711479435236	21.006300281089462
62-63	20.066557675780114	26.121389138390423	32.64701384356577	21.165039342263693
64-65	19.982018905565653	25.759640929520057	33.12911106449027	21.129229100424023
66-67	19.96264244399059	25.951137936486123	32.50467077146024	21.581548848063044
68-69	20.815487112704254	26.177032067518002	32.22348418680359	20.78399663297415
70-71	20.895339465128593	25.797221694510462	32.274205061466574	21.033233778894374
72-73	20.44095816493305	25.953710636120125	32.65227966092203	20.95305153802479
74-75	20.324957740071717	25.92954610855417	32.44946470757508	21.296031443799034
76-77	20.431545554878745	26.066683495925226	31.877193224299265	21.624577724896763
78-79	20.73928915134289	26.240491475175094	32.0429640147191	20.97725535876292
80-81	20.13462832467603	25.986514766812817	32.30552082965373	21.573336078857423
82-83	19.94253711620241	26.106668843584774	32.39921863574007	21.551575404472747
84-85	20.15182189068936	25.987614473287717	32.11158131397037	21.748982322052555
86-87	20.439967363874704	26.14568911075174	31.804175494129723	21.610168031243834
88-89	19.91669517014639	26.065692694866875	32.002163392571745	22.015448742414986
90-91	19.745420074969193	25.889658770195922	31.850060751195368	22.514860403639513
92-93	19.973541241222506	26.28702670021326	31.66084282476328	22.078589233800958
94-95	19.841049663256875	25.88013288796108	32.021419395749206	22.257398053032844
96-97	19.686427210455328	26.10307912273197	31.936361838814857	22.274131827997845
98-99	19.583046771056107	26.008604083978238	31.960426296972074	22.447922847993585
100	19.855006722384267	26.215187806745128	31.632425800687447	22.297379670183158
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6999.0
1	4223.0
2	11399.0
3	18120.5
4	9491.5
5	4039.0
6	6139.5
7	13452.0
8	20155.5
9	20015.0
10	12792.5
11	5781.0
12	4228.0
13	4250.5
14	4497.0
15	4707.5
16	4949.0
17	5363.0
18	5649.0
19	6002.5
20	6709.0
21	7453.5
22	8506.0
23	10405.5
24	12836.5
25	15525.0
26	19344.0
27	24104.0
28	30048.5
29	36151.5
30	40556.5
31	44952.5
32	49747.0
33	53791.5
34	57680.5
35	61661.5
36	64631.0
37	66647.0
38	68157.5
39	68855.5
40	69662.0
41	69573.0
42	67792.5
43	67464.0
44	67309.0
45	66515.0
46	66707.5
47	67266.5
48	68127.0
49	70577.5
50	73856.5
51	71288.5
52	64628.0
53	59523.5
54	56543.5
55	55393.5
56	51454.0
57	46221.5
58	43490.0
59	41035.5
60	35828.5
61	29241.0
62	23775.5
63	18209.5
64	13528.5
65	10362.5
66	7783.0
67	5746.5
68	4127.5
69	2953.5
70	2129.5
71	1575.0
72	1219.5
73	936.0
74	836.5
75	711.5
76	456.0
77	366.5
78	299.0
79	163.0
80	81.0
81	39.5
82	18.0
83	7.0
84	3.5
85	2.0
86	1.0
87	1.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.5
95	1.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04523222222892329
2	0.0
3	3.0154814819282195E-4
4	0.0660821227611127
5	0.004178595767814818
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.011717299472635367
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.384788360586106E-4
26-27	0.0
28-29	5.169396826162661E-4
30-31	0.0038124301592949634
32-33	2.3693068786578869E-4
34-35	0.001916984656368654
36-37	0.0043293698419112295
38-39	0.0014646624340794209
40-41	0.0010338793652325323
42-43	0.0018954455029263095
44-45	0.0
46-47	5.169396826162661E-4
48-49	6.677137567126771E-4
50-51	0.0016154365081758318
52-53	0.0012708100530983211
54-55	0.0013354275134253542
56-57	0.0065909809533573945
58-59	0.009369531747419824
60-61	0.0012492708996559767
62-63	0.0025416201061966423
64-65	0.001163114285886599
66-67	1.2923492065406654E-4
68-69	7.538703704820549E-4
70-71	4.7386137573157737E-4
72-73	0.0
74-75	2.5846984130813307E-4
76-77	0.0
78-79	6.892529101550216E-4
80-81	0.0
82-83	0.0012708100530983211
84-85	6.677137567126771E-4
86-87	0.0
88-89	0.0
90-91	2.1539153442344424E-5
92-93	2.3693068786578869E-4
94-95	0.0
96-97	4.7386137573157737E-4
98-99	6.246354498279883E-4
100	2.1539153442344427E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2321354.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.10173096629975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.08270563980673	48.32090204090547
2	13.953260050557766	17.05136683423997
3	3.6417889298256014	6.675588222787576
4	1.27159087509254	3.107856141964242
5	0.5780810763153404	1.7660877200864467
6	0.33436655834060297	1.2258225295113054
7	0.2231189117530086	0.9543066203598134
8	0.13873048236151417	0.6781338088062585
9	0.10661586029225147	0.5862972251085974
>10	0.5856272467143532	6.597965856909907
>50	0.04883271690661723	2.0558071091719587
>100	0.03174942222742758	3.564020350877902
>500	0.0023312716720939315	0.982221192105651
>1k	6.358013651163473E-4	0.520009611131368
>5k	2.1193378837211575E-4	0.9215742672577992
>10k+	3.532229806201929E-4	4.992040468775854
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29638	1.2767548594484082	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29115	1.2542249049477159	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24061	1.0365071419524985	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20209	0.870569503832677	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12627	0.5439497810329661	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9435	0.4064438254570393	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6737	0.29021855348214876	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5178	0.22305947304891885	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2456	0.10580032170879582	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	4.307830688468885E-5	0.0
8	0.0	0.0	0.0	4.307830688468885E-5	0.0
9	0.0	0.0	0.0	4.307830688468885E-5	0.0
10-11	0.0	0.0	0.0	4.307830688468885E-5	0.0
12-13	0.0	0.0	0.0	4.307830688468885E-5	0.0
14-15	0.0	0.0	0.0	4.307830688468885E-5	0.0
16-17	0.0	0.0	0.0	4.307830688468885E-5	0.0
18-19	0.0	4.307830688468885E-5	0.0	6.461746032703327E-5	0.0
20-21	0.0	4.307830688468885E-5	0.0	1.5077407409641098E-4	0.0
22-23	4.307830688468885E-5	4.307830688468885E-5	0.0	0.0011415751324442545	0.0
24-25	8.61566137693777E-5	4.307830688468885E-5	0.0	0.0027785507940624307	0.0
26-27	8.61566137693777E-5	4.307830688468885E-5	0.0	0.004415526455680607	0.0
28-29	8.61566137693777E-5	4.307830688468885E-5	0.0	0.009283375133650447	0.0
30-31	8.61566137693777E-5	4.307830688468885E-5	0.0	0.019600629632533426	0.0
32-33	8.61566137693777E-5	4.307830688468885E-5	0.0	0.03711196138115944	0.0
34-35	8.61566137693777E-5	4.307830688468885E-5	0.0	0.06125735239002754	0.0
36-37	8.61566137693777E-5	4.307830688468885E-5	0.0	0.0960000068925291	0.0
38-39	8.61566137693777E-5	4.307830688468885E-5	0.0	0.1475432010800593	0.0
40-41	8.61566137693777E-5	4.307830688468885E-5	0.0	0.2127422185500359	0.0
42-43	8.61566137693777E-5	4.307830688468885E-5	0.0	0.27779046194591606	0.0
44-45	8.61566137693777E-5	4.307830688468885E-5	0.0	0.34960199952269233	0.0
46-47	8.61566137693777E-5	4.307830688468885E-5	0.0	0.4358232307523971	0.0
48-49	1.0769576721172212E-4	4.307830688468885E-5	0.0	0.531500150343291	0.0
50-51	1.2923492065406654E-4	4.307830688468885E-5	0.0	0.6351680958612947	0.0
52-53	1.723132275387554E-4	4.307830688468885E-5	0.0	0.7367252043419488	0.0
54-55	1.723132275387554E-4	4.307830688468885E-5	0.0	0.8459502514480772	0.0
56-57	2.1539153442344424E-4	4.307830688468885E-5	0.0	0.9781791144306297	0.0
58-59	2.5846984130813307E-4	4.307830688468885E-5	0.0	1.1227068340287607	0.0
60-61	2.5846984130813307E-4	4.307830688468885E-5	0.0	1.2728562726753436	0.0
62-63	2.5846984130813307E-4	4.307830688468885E-5	0.0	1.4224456933324259	0.0
64-65	2.5846984130813307E-4	8.61566137693777E-5	0.0	1.5897833764259997	0.0
66-67	2.5846984130813307E-4	8.61566137693777E-5	0.0	1.7847557933861014	0.0
68-69	2.5846984130813307E-4	8.61566137693777E-5	0.0	1.9945256087610936	0.0
70-71	2.5846984130813307E-4	8.61566137693777E-5	0.0	2.219932849535228	0.0
72-73	2.5846984130813307E-4	8.61566137693777E-5	0.0	2.481762798780367	0.0
74-75	3.0154814819282195E-4	8.61566137693777E-5	0.0	2.73894029088196	0.0
76-77	3.0154814819282195E-4	8.61566137693777E-5	0.0	3.001545649651023	0.0
78-79	3.230873016351664E-4	1.2923492065406654E-4	0.0	3.301090656573707	0.0
80-81	3.446264550775108E-4	1.2923492065406654E-4	0.0	3.630187382019287	0.0
82-83	3.446264550775108E-4	1.2923492065406654E-4	0.0	3.979380137626575	0.0
84-85	3.446264550775108E-4	1.2923492065406654E-4	0.0	4.321874216513294	0.0
86-87	3.446264550775108E-4	1.2923492065406654E-4	0.0	4.688815234557073	0.0
88	3.446264550775108E-4	1.2923492065406654E-4	0.0	4.986357100209619	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8925	0.0	56.503315	1
TACCTGG	2455	0.0	46.930172	2
TATAACG	250	0.0	45.14491	2
ATAACGC	280	0.0	43.666958	3
GTATCAA	16525	0.0	42.38777	1
GCGGTAT	100	7.003109E-10	42.33795	1
GTACCTG	2825	0.0	40.964157	1
TCTGTCG	815	0.0	34.030125	8
ACCTGGG	3315	0.0	33.762222	3
GTCTCGC	750	0.0	31.361448	1
ATCAACG	22605	0.0	31.01778	3
TATCAAC	22635	0.0	30.914341	2
TCAACGC	22835	0.0	30.725954	4
GTGGTAT	3485	0.0	29.831623	1
CAACGCA	23770	0.0	29.486115	5
GTACGGA	65	0.006150509	28.927824	6
CATGGGT	5020	0.0	28.852575	4
AACGCAG	24580	0.0	28.705688	6
TAACGCA	435	0.0	28.107464	4
TGGTATC	3590	0.0	27.90119	2
>>END_MODULE