FastQCFastQC Report
Wed 25 May 2016
SRR2431431_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431431_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36234
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3701.0211403654026605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.16835016835016833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.10487387536567865No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTCA156.8478764E-494.06
GGTATCA353.7594873E-880.571431
CCTTATA200.002146048770.499992
CAGTCTG250.005195287556.3999989
AAGTGTT250.005195287556.3999989
AATACTC250.005195287556.3999985
ATCAACG708.302959E-847.04
GTATCAA652.7105816E-643.3846172
TATCAAC704.511894E-640.2857133
CAACGCA608.655844E-539.1666686
TCAACGC651.3866392E-436.1538475
TTTAATG600.004108454631.3333343
TACATGG600.004108454631.3333342
AACGCAG753.2137777E-431.3333347
AAAATCC404.7372817E-429.37499816-17
CGCAGAG856.688511E-427.647069
CAGAAGA350.00878243426.85714322-23
ACGCAGA950.001280348324.7368438
GCAGAGT1050.002292428422.3809533
CAGAGTA1050.002292428422.3809534
Produced by FastQC (version 0.11.5)