##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431431_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 36234 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 17.413479052823316 23.0 2.0 31.0 2.0 31.0 2 17.426781475961803 22.0 2.0 31.0 2.0 33.0 3 17.60683336093172 23.0 2.0 31.0 2.0 34.0 4 19.536071093448143 25.0 2.0 35.0 2.0 37.0 5 19.42415962907766 25.0 2.0 35.0 2.0 37.0 6 19.365789037920184 25.0 2.0 35.0 2.0 37.0 7 19.26996743390186 25.0 2.0 35.0 2.0 37.0 8 19.260694375448473 25.0 2.0 35.0 2.0 37.0 9 19.773389634045373 25.0 2.0 37.0 2.0 39.0 10-11 19.605646630236794 24.5 2.0 37.0 2.0 39.0 12-13 19.431293812441353 23.0 2.0 37.0 2.0 39.0 14-15 19.691063641883314 22.0 2.0 37.0 2.0 40.0 16-17 19.497764530551414 20.5 2.0 37.0 2.0 40.0 18-19 19.167439421537782 18.0 2.0 37.0 2.0 40.0 20-21 18.967613291383785 18.0 2.0 37.0 2.0 40.0 22-23 18.73759452447977 17.0 2.0 37.0 2.0 40.0 24-25 18.475409836065573 13.0 2.0 37.0 2.0 40.0 26-27 18.152867472539604 10.0 2.0 36.5 2.0 40.0 28-29 17.911215984986477 8.5 2.0 36.0 2.0 40.0 30-31 17.756306231716067 7.0 2.0 36.0 2.0 40.0 32-33 17.510625379477837 2.0 2.0 36.0 2.0 40.0 34-35 17.32254236352597 2.0 2.0 36.0 2.0 40.0 36-37 17.212190208091847 2.0 2.0 36.0 2.0 40.0 38-39 17.063917867196558 2.0 2.0 35.5 2.0 40.0 40-41 16.911188386598223 2.0 2.0 35.0 2.0 40.0 42-43 16.735179665507534 2.0 2.0 35.0 2.0 40.0 44-45 16.589680962631782 2.0 2.0 35.0 2.0 40.0 46-47 16.411823149528068 2.0 2.0 35.0 2.0 40.0 48-49 16.252194071866203 2.0 2.0 35.0 2.0 40.0 50-51 16.11210465308826 2.0 2.0 35.0 2.0 40.0 52-53 15.975865209471767 2.0 2.0 35.0 2.0 40.0 54-55 15.875227686703095 2.0 2.0 34.0 2.0 40.0 56-57 15.716950930065684 2.0 2.0 34.0 2.0 40.0 58-59 15.531793343268752 2.0 2.0 34.0 2.0 39.5 60-61 15.354763481812661 2.0 2.0 34.0 2.0 39.0 62-63 15.156400066236131 2.0 2.0 33.5 2.0 39.0 64-65 15.034084009493846 2.0 2.0 33.0 2.0 39.0 66-67 14.859124027156813 2.0 2.0 33.0 2.0 39.0 68-69 14.715391621129326 2.0 2.0 33.0 2.0 38.5 70-71 14.511798310978637 2.0 2.0 33.0 2.0 37.5 72-73 14.334451068057625 2.0 2.0 33.0 2.0 37.0 74-75 14.141165755919854 2.0 2.0 32.0 2.0 37.0 76-77 13.917025445713971 2.0 2.0 32.0 2.0 36.0 78-79 13.730957112104653 2.0 2.0 32.0 2.0 36.0 80-81 13.555734945079207 2.0 2.0 32.0 2.0 35.0 82-83 13.376041839156594 2.0 2.0 32.0 2.0 35.0 84-85 13.210810288679141 2.0 2.0 31.0 2.0 35.0 86-87 13.042736104211514 2.0 2.0 31.0 2.0 35.0 88-89 12.853190373682178 2.0 2.0 31.0 2.0 35.0 90-91 12.678851355080862 2.0 2.0 31.0 2.0 35.0 92-93 12.560343875917646 2.0 2.0 31.0 2.0 35.0 94-95 12.410043053485676 2.0 2.0 31.0 2.0 35.0 96-97 12.276756637412376 2.0 2.0 31.0 2.0 35.0 98-99 12.11220124744715 2.0 2.0 31.0 2.0 35.0 100 12.001379919412706 2.0 2.0 31.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.2119639837041092 1101 2 -0.49763393882174967 1101 3 -1.6962405069512059 1101 4 -1.5064721095104048 1101 5 -2.5076901638577773 1101 6 -1.3649994088773987 1101 7 -1.8369677717466857 1101 8 -0.5749436740099156 1101 9 -0.9328905362923194 1101 10-11 -1.6290745061476777 1101 12-13 -0.6634224899972381 1101 14-15 0.042807777631352906 1101 16-17 -0.5936420899635309 1101 18-19 -0.6207619173085703 1101 20-21 -0.9496164396071123 1101 22-23 -1.4840999303400686 1101 24-25 -1.1047699012489716 1101 26-27 -1.3266305124959779 1101 28-29 -0.7895986107575688 1101 30-31 0.07730533875494316 1101 32-33 -0.8792689397753612 1101 34-35 -1.2939841515669563 1101 36-37 -0.8309980536554633 1101 38-39 -0.537339497083881 1101 40-41 -0.8183819401866508 1101 42-43 -1.3311655004882894 1101 44-45 -0.44658939993269087 1101 46-47 -0.7593856073331153 1101 48-49 -0.23720673299534667 1101 50-51 -0.8172501882784324 1101 52-53 -0.7054942487028519 1101 54-55 -0.7059266850648669 1101 56-57 -0.8017716465006473 1101 58-59 -0.9478461087275729 1101 60-61 -1.0335608646246062 1101 62-63 -1.100072614629502 1101 64-65 -1.7829316137223454 1101 66-67 -0.6635136818456164 1101 68-69 -1.6341428995451857 1101 70-71 -1.0790478403848969 1101 72-73 -1.098474101747538 1101 74-75 -0.6744008581953267 1101 76-77 -0.5930736529997542 1101 78-79 -0.49420935970023194 1101 80-81 -0.6932239175451507 1101 82-83 -0.921979890067746 1101 84-85 -1.504133789778347 1101 86-87 -1.4171906938823753 1101 88-89 -1.2632290523503116 1101 90-91 -0.6075813495314133 1101 92-93 -1.3857648642645746 1101 94-95 -0.8100191253741524 1101 96-97 -1.48091934741894 1101 98-99 -1.2265228131966737 1101 100 -1.107469828972791 1102 1 -2.1632646491608565 1102 2 -1.149176588549519 1102 3 -0.5564945904357792 1102 4 -1.4713843902121582 1102 5 -1.9015195649467067 1102 6 -0.9663303223680231 1102 7 -1.8245660778567903 1102 8 -1.552257648601568 1102 9 -2.100464643975318 1102 10-11 -1.8597157644658893 1102 12-13 -1.346574335127304 1102 14-15 -1.8721952471720371 1102 16-17 -2.1219240016392718 1102 18-19 -2.514288838058725 1102 20-21 -2.722907425693016 1102 22-23 -2.709447782729665 1102 24-25 -2.2470869006440104 1102 26-27 -2.0283848984608888 1102 28-29 -1.1867552955730574 1102 30-31 -0.9429608439431796 1102 32-33 -1.5869216923464435 1102 34-35 -1.6971904431580036 1102 36-37 -1.1973017439155953 1102 38-39 -1.4841029574589584 1102 40-41 -1.714177463477636 1102 42-43 -1.680379051607467 1102 44-45 -2.1205155947300316 1102 46-47 -2.0121079303821183 1102 48-49 -1.7455249423117394 1102 50-51 -1.8211824326825443 1102 52-53 -2.096450086573391 1102 54-55 -2.7481226923243955 1102 56-57 -3.3147783010681007 1102 58-59 -3.3068902708570356 1102 60-61 -3.413929890637915 1102 62-63 -2.666769529330047 1102 64-65 -2.6974809180175647 1102 66-67 -2.359520941373747 1102 68-69 -2.6244635287042914 1102 70-71 -2.2980436056601548 1102 72-73 -2.848776582086316 1102 74-75 -2.670771094129993 1102 76-77 -2.319328946405683 1102 78-79 -2.0905493476010157 1102 80-81 -1.5599813283134516 1102 82-83 -2.152469908216567 1102 84-85 -2.4812965242006086 1102 86-87 -2.642387306102581 1102 88-89 -2.106695477032707 1102 90-91 -1.864387157153919 1102 92-93 -2.0241496192555015 1102 94-95 -2.275233886414684 1102 96-97 -2.63820912358349 1102 98-99 -2.8169644344912896 1102 100 -3.552418407315198 1103 1 -5.597079999550177 1103 2 -4.754849558516147 1103 3 -4.91355799421776 1103 4 -5.358481164405706 1103 5 -4.833666394757607 1103 6 -4.467998842946443 1103 7 -5.9241211390358774 1103 8 -4.759154200325707 1103 9 -5.265092007712806 1103 10-11 -5.29659006924898 1103 12-13 -3.8991327333475496 1103 14-15 -4.13220637064256 1103 16-17 -3.9678639348984497 1103 18-19 -4.655000740172184 1103 20-21 -4.977912987428276 1103 22-23 -5.594597949581724 1103 24-25 -6.2623816726128645 1103 26-27 -6.471933285557663 1103 28-29 -7.179525039733235 1103 30-31 -6.638455838381443 1103 32-33 -6.503773750188492 1103 34-35 -6.8896264832024965 1103 36-37 -6.7782249919689885 1103 38-39 -6.328652456902784 1103 40-41 -6.4035545491283585 1103 42-43 -6.561357917013474 1103 44-45 -6.0504377304363715 1103 46-47 -5.514054537723609 1103 48-49 -6.542243518507515 1103 50-51 -6.682417137910576 1103 52-53 -6.5825457484198875 1103 54-55 -6.975875751278791 1103 56-57 -6.201318901735508 1103 58-59 -6.393375254171829 1103 60-61 -6.151415986299762 1103 62-63 -6.4281710866159205 1103 64-65 -6.10904932736128 1103 66-67 -6.169031508670743 1103 68-69 -5.613061971418416 1103 70-71 -6.369511903769164 1103 72-73 -6.478921187203113 1103 74-75 -6.141293897244564 1103 76-77 -5.675558089898452 1103 78-79 -5.928702851494232 1103 80-81 -6.199024709848491 1103 82-83 -6.322659007215454 1103 84-85 -6.139249805624414 1103 86-87 -6.180207105880111 1103 88-89 -6.0149268452195805 1103 90-91 -5.607156901314097 1103 92-93 -5.516919363415679 1103 94-95 -5.768281717337933 1103 96-97 -5.305339268188606 1103 98-99 -5.391491686994071 1103 100 -4.979003501864698 1104 1 -1.251038943831702 1104 2 -1.5749864109110643 1104 3 -1.3940076520867741 1104 4 -2.016838935666705 1104 5 -1.7740378512580968 1104 6 -2.8937075428486914 1104 7 -2.178275586738714 1104 8 -2.168245109416617 1104 9 -2.3643099940275647 1104 10-11 -1.7789947613310915 1104 12-13 -1.3771385984501876 1104 14-15 -1.8455494791469604 1104 16-17 -2.0370232701868183 1104 18-19 -1.9832021086961333 1104 20-21 -1.5580693901653255 1104 22-23 -1.887608702269894 1104 24-25 -1.760671017676401 1104 26-27 -2.429900049976041 1104 28-29 -2.180746936116421 1104 30-31 -2.416315075918101 1104 32-33 -1.4534316192012007 1104 34-35 -1.2784339123325061 1104 36-37 -1.6675211796522724 1104 38-39 -1.2678020170200863 1104 40-41 -1.7745222910638425 1104 42-43 -1.9870666169157225 1104 44-45 -1.6176420315116395 1104 46-47 -1.6218257987206766 1104 48-49 -1.8711780248613756 1104 50-51 -2.0831991515958173 1104 52-53 -1.6536601179213548 1104 54-55 -1.8654163182387098 1104 56-57 -1.2304000356553537 1104 58-59 -0.7572559970848296 1104 60-61 -1.2237522521844149 1104 62-63 -1.4836451824125962 1104 64-65 -1.9707306567845464 1104 66-67 -1.0470862496287108 1104 68-69 -0.5878909059247714 1104 70-71 -0.9853476808952646 1104 72-73 -0.8432906886693878 1104 74-75 -0.7744806030119147 1104 76-77 -0.7840624887254322 1104 78-79 -1.8546559306731183 1104 80-81 -1.6242446511974649 1104 82-83 -1.3888857911841725 1104 84-85 -1.134117840815028 1104 86-87 -1.016472990533094 1104 88-89 -0.6312513808989539 1104 90-91 -0.547772737091222 1104 92-93 -0.4272321688897751 1104 94-95 -0.39853587178563465 1104 96-97 -1.0506960619324968 1104 98-99 -0.47213684828439284 1104 100 -0.6872146455597132 1105 1 -2.895599301506815 1105 2 -3.2051404099175222 1105 3 -2.8696658736466336 1105 4 -4.422204062343306 1105 5 -3.464549525376327 1105 6 -3.3077661618253416 1105 7 -3.1176695261326515 1105 8 -1.8001378068830824 1105 9 -2.668582224529306 1105 10-11 -3.1783986361448022 1105 12-13 -3.482950819016507 1105 14-15 -3.8880233986700574 1105 16-17 -3.381533950763071 1105 18-19 -3.1994216814730816 1105 20-21 -2.7349198620977653 1105 22-23 -3.5378819263136094 1105 24-25 -3.5886640628825646 1105 26-27 -3.8808439148543314 1105 28-29 -3.537801084154175 1105 30-31 -3.098133257736283 1105 32-33 -3.349358097094891 1105 34-35 -2.6225720146673304 1105 36-37 -3.2159563510382654 1105 38-39 -3.2692923299858094 1105 40-41 -3.5040304877851547 1105 42-43 -3.3396498260563092 1105 44-45 -3.108079189981586 1105 46-47 -2.728755754011287 1105 48-49 -2.7770399225265514 1105 50-51 -2.6928613473235856 1105 52-53 -3.1924082550301485 1105 54-55 -2.6450983848060243 1105 56-57 -3.0069772835440745 1105 58-59 -3.100276364695361 1105 60-61 -3.037587324216208 1105 62-63 -3.1816084213594404 1105 64-65 -3.359578148091053 1105 66-67 -3.8434101443132622 1105 68-69 -3.359624636674896 1105 70-71 -2.9365399312678413 1105 72-73 -2.7881491089376453 1105 74-75 -3.5294483128976566 1105 76-77 -3.691290506609187 1105 78-79 -3.6802893136835486 1105 80-81 -3.366793086368851 1105 82-83 -3.323187827945226 1105 84-85 -2.9746543534826895 1105 86-87 -2.8720368256051234 1105 88-89 -2.61051119212598 1105 90-91 -2.7974001588497917 1105 92-93 -2.674375735705473 1105 94-95 -2.5465736263807663 1105 96-97 -2.062742758405422 1105 98-99 -2.2098417663171794 1105 100 -2.340716881029161 1106 1 0.3244618265843222 1106 2 -0.16846355523566814 1106 3 0.08698875263844741 1106 4 -0.0646047291952101 1106 5 0.15867914925551574 1106 6 -0.5811754421809958 1106 7 -0.38885596681061685 1106 8 0.11372715560649738 1106 9 0.2969637604665287 1106 10-11 -1.2520302004682264 1106 12-13 -0.8879244227953365 1106 14-15 0.481106915306043 1106 16-17 0.5456680497926385 1106 18-19 -0.174136880139379 1106 20-21 0.2249300962239893 1106 22-23 -0.4864787587670705 1106 24-25 -0.03712196785616584 1106 26-27 -0.2317747289693628 1106 28-29 0.031245873334015073 1106 30-31 1.1391548778569351 1106 32-33 0.8480578517797959 1106 34-35 0.853949241237089 1106 36-37 0.8471167248161393 1106 38-39 -0.3140269784992853 1106 40-41 0.6322573699531056 1106 42-43 0.5330926023960387 1106 44-45 0.38094553910982754 1106 46-47 0.904753554129865 1106 48-49 0.20406594021309843 1106 50-51 0.07332370407958066 1106 52-53 -0.16906843841442054 1106 54-55 -0.5600747671344468 1106 56-57 -0.9544626961586911 1106 58-59 -0.7554583596939715 1106 60-61 -0.8830999666168751 1106 62-63 -1.2057818028543021 1106 64-65 -1.2335709238621 1106 66-67 -1.548883887019569 1106 68-69 -1.4879936280613908 1106 70-71 -1.2977513788921833 1106 72-73 -1.1838145715661526 1106 74-75 -0.9808236949482279 1106 76-77 -0.4531688061368353 1106 78-79 -0.5480303528610992 1106 80-81 -0.4143062844794372 1106 82-83 -0.5022653494789857 1106 84-85 -0.5185554371170316 1106 86-87 -0.5887636941797272 1106 88-89 0.019108146579215557 1106 90-91 0.2773428253124752 1106 92-93 -0.2341145520433443 1106 94-95 -0.2416278080979115 1106 96-97 -0.03592975479330818 1106 98-99 -0.48908600082676656 1106 100 -0.3721144914765073 1107 1 -1.1836988764916363 1107 2 -1.787107623032277 1107 3 -0.9428292009083208 1107 4 -0.5454584642862308 1107 5 -1.2757213529415985 1107 6 -2.3162664654076117 1107 7 -1.1361880446511705 1107 8 -2.185080126251634 1107 9 -1.6521887819063537 1107 10-11 -2.6729341552704824 1107 12-13 -2.3510443223559108 1107 14-15 -1.8329232165206975 1107 16-17 -1.4747337078972542 1107 18-19 -0.7879159134099396 1107 20-21 -0.5875306696266058 1107 22-23 -1.3320531467143404 1107 24-25 -1.173970680976069 1107 26-27 -0.7320886021645912 1107 28-29 -0.5376492930187773 1107 30-31 -0.12591103551406135 1107 32-33 -0.8212767370463148 1107 34-35 -0.22624536014395602 1107 36-37 -0.11364912578022057 1107 38-39 0.05963906042099154 1107 40-41 -0.5060037725453235 1107 42-43 -0.6040699839190893 1107 44-45 -1.126901290770899 1107 46-47 -1.6857988626937406 1107 48-49 -1.247440651124002 1107 50-51 -0.8045796229762878 1107 52-53 -1.556858771120007 1107 54-55 -2.042183995006388 1107 56-57 -1.4635650188203346 1107 58-59 -1.7261112159400476 1107 60-61 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4.3652321228157085 2215 28-29 4.271058350795247 2215 30-31 4.189100784816908 2215 32-33 2.513041623867789 2215 34-35 2.7881360425353208 2215 36-37 3.327643230405755 2215 38-39 2.3482521415872633 2215 40-41 1.5765415387233936 2215 42-43 1.8931719388547492 2215 44-45 2.8486700252259496 2215 46-47 2.306123911143928 2215 48-49 2.216507339419735 2215 50-51 2.2419136788361627 2215 52-53 2.268003325018686 2215 54-55 2.2722368087761193 2215 56-57 3.0395870694381344 2215 58-59 3.6424861047849326 2215 60-61 2.330567541497082 2215 62-63 2.6591659072070026 2215 64-65 2.5029959711974 2215 66-67 2.436150371905784 2215 68-69 3.203856353907595 2215 70-71 3.166006284288489 2215 72-73 3.001003315199082 2215 74-75 2.8552743937643577 2215 76-77 2.890502308179789 2215 78-79 2.8648798526924537 2215 80-81 2.7901654068296153 2215 82-83 2.4330033126198245 2215 84-85 1.93744889032617 2215 86-87 2.9256611164946325 2215 88-89 2.549760869262231 2215 90-91 2.614703084959066 2215 92-93 2.981536367538336 2215 94-95 2.3139077129983754 2215 96-97 3.0239332095052855 2215 98-99 2.144884428311352 2215 100 2.57487432929522 2216 1 3.5356743428815847 2216 2 3.046225710301055 2216 3 4.280817522738403 2216 4 4.759384840557072 2216 5 4.111743034522789 2216 6 4.16806401892287 2216 7 4.677202268739144 2216 8 3.535717594546089 2216 9 4.8862727565551864 2216 10-11 3.0193735370372075 2216 12-13 2.9317192104076177 2216 14-15 4.331606698009217 2216 16-17 4.612160878997333 2216 18-19 4.708522833028809 2216 20-21 4.161044828948892 2216 22-23 4.253416938755745 2216 24-25 4.525459518454134 2216 26-27 4.753666383590396 2216 28-29 1.2009546955852102 2216 30-31 2.837764178161155 2216 32-33 2.0640508982813195 2216 34-35 2.512358628460035 2216 36-37 3.5131315010004087 2216 38-39 3.2170464678284 2216 40-41 3.1357341191925663 2216 42-43 2.888807508958404 2216 44-45 3.4656913018216926 2216 46-47 2.928873501978625 2216 48-49 3.97264481896147 2216 50-51 3.108525790129125 2216 52-53 3.9652210009597653 2216 54-55 3.205679252856859 2216 56-57 3.8797398244845063 2216 58-59 4.5865668467380125 2216 60-61 4.315292036859873 2216 62-63 4.300737101969689 2216 64-65 4.339875403821512 2216 66-67 4.980885778343316 2216 68-69 4.581106763072897 2216 70-71 3.924325978778395 2216 72-73 3.70449485911616 2216 74-75 2.7156126370813247 2216 76-77 3.5277090730461307 2216 78-79 4.225763649746462 2216 80-81 2.996384719431905 2216 82-83 2.401906749608367 2216 84-85 2.4101712034740146 2216 86-87 3.3162775922174905 2216 88-89 2.790077290429716 2216 90-91 2.1515279622094763 2216 92-93 2.280227038569432 2216 94-95 1.6306016573518942 2216 96-97 2.2808890196525855 2216 98-99 2.245811869664326 2216 100 2.162435704090637 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 13997.0 3 991.0 4 666.0 5 644.0 6 590.0 7 622.0 8 558.0 9 541.0 10 472.0 11 327.0 12 375.0 13 343.0 14 289.0 15 297.0 16 268.0 17 267.0 18 243.0 19 274.0 20 251.0 21 252.0 22 245.0 23 322.0 24 389.0 25 381.0 26 427.0 27 433.0 28 517.0 29 565.0 30 568.0 31 668.0 32 755.0 33 832.0 34 964.0 35 1136.0 36 1429.0 37 1882.0 38 1899.0 39 553.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.66904012805652 23.734613898548325 12.399955842578793 26.19639013081636 2 16.042943092123423 26.331622233261577 31.829221173483468 25.796213501131533 3 19.128442898934704 28.531213777115415 26.955345807804825 25.38499751614506 4 15.562731136501629 21.358392669868078 32.34807087266104 30.730805320969257 5 13.920627035381134 38.59910581222057 29.46127946127946 18.018987691118838 6 29.055583153943807 37.6635204504057 16.08710051333002 17.193795882320472 7 26.01700060716454 33.64795495943037 21.70613236187007 18.628912071535023 8 24.032676491692886 38.5632279074902 18.780703206932714 18.623392393884195 9 26.212949163768833 21.744770105425843 18.474361097311917 33.5679196334934 10-11 23.034994756306233 29.745542860296958 25.77827454876635 21.44118783463046 12-13 24.177568030027047 28.16139537451013 25.171110007175578 22.489926588287243 14-15 23.04603411160788 29.57305293370867 23.19920516641828 24.181707788265168 16-17 23.451730418943534 31.756085444610033 22.865264668543357 21.926919467903076 18-19 22.187724236904565 30.23817409063311 25.394656952034 22.179444720428325 20-21 22.683115306066128 29.55097422310537 25.262184688414198 22.503725782414307 22-23 23.396533642435283 30.312689738919246 24.707457084506267 21.583319534139207 24-25 22.512315950708597 29.51964342390329 25.207335752825422 22.760704872562684 26-27 22.954959430369268 28.539493293591654 25.3063421096208 23.19920516641828 28-29 22.715354254643813 29.06350914962325 25.276696751400735 22.9444398443322 30-31 22.13390737980902 29.886294640393 24.692277970966494 23.287520008831482 32-33 22.179444720428325 28.035822707953855 25.31186178727162 24.472870784346192 34-35 24.194127062979522 26.86427112656621 25.38499751614506 23.556604294309214 36-37 23.15090798697356 27.297565822155985 25.405696307335653 24.1458298835348 38-39 21.751669702489373 27.55975051057018 26.076337141910912 24.612242645029532 40-41 23.443450902467298 26.71523982999393 26.49859248219904 23.342716785339736 42-43 22.158745929237732 27.127835734393113 26.924987580725286 23.788430755643873 44-45 22.977038140972567 26.9719048407573 26.544129822818345 23.506927195451784 46-47 23.291659767069604 27.487994701109454 25.634762929844896 23.585582601976043 48-49 23.309598719434785 27.428658166363086 25.09797427830215 24.163768835899983 50-51 23.506927195451784 27.227189932107965 25.49815090798697 23.767731964453276 52-53 23.70977534911961 27.65772478887233 24.87304741403102 23.75945244797704 54-55 23.5096870342772 27.599768173538664 24.787492410443228 24.103052381740905 56-57 23.704255671468786 27.29204614450516 25.055196776508254 23.9485014075178 58-59 24.477010542584313 26.942926533090468 24.722636198046033 23.857426726279186 60-61 24.3210796489485 27.783297455428603 24.38869570017111 23.506927195451784 62-63 23.97471987635922 27.87575205607993 24.55980570734669 23.589722360214164 64-65 23.771871722691394 27.521112767014404 24.68261853507755 24.024396975216646 66-67 24.06027487994701 27.17889275266324 24.744714908649335 24.01611745874041 68-69 24.48666997847326 27.391400342220013 24.619142242093062 23.50278743721367 70-71 24.48666997847326 27.214770657393604 24.409394491361706 23.889164872771428 72-73 24.448706979825023 27.183782739491626 24.89167333646124 23.475836944222117 74-75 25.07865540652426 26.904288789534693 24.632941436220122 23.384114367720926 76-77 25.336700336700336 27.09885742672628 23.94988132693051 23.614560909642876 78-79 25.08969476182591 27.24098912623503 24.242424242424242 23.426891869514822 80-81 24.38179610310758 27.530772202903353 24.71711652039521 23.370315173593863 82-83 25.277363801953967 26.98570403488436 24.533587238505273 23.2033449246564 84-85 24.75023458630016 27.056079924932387 24.242424242424242 23.951261246343215 86-87 25.00137991941271 27.278246950378097 24.029916652867474 23.690456477341723 88-89 24.971021692333167 26.88634983716951 24.412154330187118 23.730474140310207 90-91 24.71711652039521 26.916708064249047 24.581884417949993 23.78429099740575 92-93 24.643980791521773 27.37898106750566 24.815090798697355 23.16194734227521 94-95 24.408014571949 27.165093558536178 24.89512612463432 23.5317657448805 96-97 24.617762322680356 27.653585030634208 24.72815587569686 23.000496770988573 98-99 24.485290059060553 27.138875089694764 24.889606446983496 23.48622840426119 100 24.04647568581995 28.03444278854115 24.286581663630844 23.632499862008057 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 274.0 1 170.5 2 61.5 3 49.5 4 41.0 5 40.5 6 36.5 7 45.0 8 59.0 9 65.0 10 63.0 11 48.5 12 42.0 13 35.5 14 32.0 15 38.5 16 40.5 17 43.5 18 45.5 19 48.5 20 54.0 21 68.5 22 81.0 23 90.0 24 112.5 25 135.0 26 161.5 27 202.0 28 251.5 29 321.5 30 373.5 31 413.0 32 471.0 33 557.5 34 644.5 35 678.0 36 742.5 37 808.0 38 856.5 39 950.0 40 973.5 41 979.5 42 1003.0 43 1007.5 44 1054.0 45 1114.5 46 1133.0 47 1123.5 48 1150.0 49 1141.5 50 1110.5 51 1096.0 52 1061.5 53 1048.0 54 1031.5 55 1006.5 56 964.5 57 909.0 58 857.0 59 805.0 60 748.0 61 668.5 62 601.0 63 549.5 64 506.5 65 434.5 66 380.0 67 342.5 68 289.5 69 260.5 70 228.5 71 198.0 72 182.0 73 168.0 74 132.0 75 119.0 76 118.0 77 105.0 78 89.0 79 68.0 80 58.5 81 45.5 82 38.5 83 33.0 84 34.0 85 34.5 86 24.5 87 21.0 88 19.0 89 12.0 90 8.5 91 11.5 92 10.0 93 3.0 94 2.0 95 2.0 96 1.5 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0013799194127062978 26-27 0.0 28-29 0.008279516476237788 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0027598388254125956 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 36234.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.18496439807916 #Duplication Level Percentage of deduplicated Percentage of total 1 99.09127051740785 96.30181597394713 2 0.6730277730448118 1.3081636032455706 3 0.1192707445902198 0.34773969200198707 4 0.04827625376270801 0.18766904012805652 5 0.014198898165502358 0.0689959706353149 6 0.008519338899301414 0.04967709885742673 7 0.011359118532401886 0.07727548711155269 8 0.0028397796331004715 0.022078710603300765 9 0.005679559266200943 0.04967709885742673 >10 0.0198784574317033 0.39741679085941384 >50 0.0028397796331004715 0.16835016835016833 >100 0.0028397796331004715 1.0211403654026605 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 370 1.0211403654026605 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 61 0.16835016835016833 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38 0.10487387536567865 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.002759838825412596 0.0 32-33 0.0 0.0 0.0 0.008279516476237788 0.0 34-35 0.0 0.0 0.0 0.023458630016007065 0.0 36-37 0.0 0.0 0.0 0.041397582381188944 0.0 38-39 0.0 0.0 0.0 0.0703758900480212 0.0 40-41 0.0 0.0 0.0 0.09245460065132197 0.0 42-43 0.0 0.0 0.0 0.12281282773086051 0.0 44-45 0.0 0.0 0.0 0.16007065187393055 0.0 46-47 0.0 0.0 0.0 0.19870839542970692 0.0 48-49 0.0 0.0 0.0 0.23044654192195174 0.0 50-51 0.0 0.0 0.0 0.2815035601920848 0.0 52-53 0.0 0.0 0.0 0.32428106198598 0.0 54-55 0.0 0.0 0.0 0.37533808025611304 0.0 56-57 0.0 0.0 0.0 0.44571397030413423 0.0 58-59 0.0 0.0 0.0 0.5312689738919247 0.0 60-61 0.0 0.0 0.0 0.6182038968924215 0.0 62-63 0.0 0.0 0.0 0.6871998675277364 0.0 64-65 0.0 0.0 0.0 0.7865540652425898 0.0 66-67 0.0 0.0 0.0 0.8638295523541426 0.0 68-69 0.002759838825412596 0.0 0.0 0.9783628636087652 0.0 70-71 0.002759838825412596 0.0 0.0 1.074957222498206 0.0 72-73 0.002759838825412596 0.0 0.0 1.195010211403654 0.0 74-75 0.002759838825412596 0.0 0.0 1.3343820720869901 0.0 76-77 0.002759838825412596 0.0 0.0 1.4599547386432632 0.0 78-79 0.002759838825412596 0.0 0.0 1.6186454711044873 0.0 80-81 0.002759838825412596 0.0 0.0 1.8283932218358447 0.0 82-83 0.002759838825412596 0.0 0.0 2.032621294916377 0.0 84-85 0.002759838825412596 0.0 0.0 2.2161505768063146 0.0 86-87 0.002759838825412596 0.0 0.0 2.40795937517249 0.0 88 0.002759838825412596 0.0 0.0 2.541811558205001 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACTCA 15 6.8478764E-4 94.0 6 GGTATCA 35 3.7594873E-8 80.57143 1 CCTTATA 20 0.0021460487 70.49999 2 CAGTCTG 25 0.0051952875 56.399998 9 AAGTGTT 25 0.0051952875 56.399998 9 AATACTC 25 0.0051952875 56.399998 5 ATCAACG 70 8.302959E-8 47.0 4 GTATCAA 65 2.7105816E-6 43.384617 2 TATCAAC 70 4.511894E-6 40.285713 3 CAACGCA 60 8.655844E-5 39.166668 6 TCAACGC 65 1.3866392E-4 36.153847 5 TTTAATG 60 0.0041084546 31.333334 3 TACATGG 60 0.0041084546 31.333334 2 AACGCAG 75 3.2137777E-4 31.333334 7 AAAATCC 40 4.7372817E-4 29.374998 16-17 CGCAGAG 85 6.688511E-4 27.64706 9 CAGAAGA 35 0.008782434 26.857143 22-23 ACGCAGA 95 0.0012803483 24.736843 8 GCAGAGT 105 0.0022924284 22.380953 3 CAGAGTA 105 0.0022924284 22.380953 4 >>END_MODULE