FastQCFastQC Report
Wed 25 May 2016
SRR2431431_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431431_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36234
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2690.7423966440359883No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.43329469558977757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.31186178727162334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.2621846884141966No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.23734613898548323No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.16007065187393055No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT500.1379919412706298No Hit
CCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCT440.12143290831815423No Hit
CCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCC430.11867306949274162No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG370.10211403654026605RNA PCR Primer, Index 23 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCCA200.002146048770.499994
GGTATCA551.240187E-859.818181
GGCATTG250.005195287556.3999988
GTATCAA901.2543751E-841.7777751
TATCAAC1550.039.4193571
CATGGGA608.655844E-539.1666684
GTACATG1950.036.1538471
ATCAACG1700.035.9411772
CATGGGT550.002682554834.1818164
TACATGG2100.033.571432
ACATGGG2100.033.571433
TCAACGC1850.033.0270273
CAACGCA2000.030.554
AACGCAG2051.8189894E-1229.8048785
ACGCAGA2253.6379788E-1227.1555566
CGCAGAG2253.6379788E-1227.1555567
CATGGGG1057.228351E-526.8571434
GCAGAGT2453.074092E-1023.0204098
CAGAGTA2701.038643E-920.888899
AGAGTAC2700.017.40740810-11
Produced by FastQC (version 0.11.5)