##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431431_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 36234 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.187393056245515 31.0 31.0 34.0 30.0 34.0 2 31.42758182922117 31.0 31.0 34.0 30.0 34.0 3 31.57625434674615 31.0 31.0 34.0 30.0 34.0 4 35.38957884859524 37.0 35.0 37.0 33.0 37.0 5 35.21730970911299 37.0 35.0 37.0 33.0 37.0 6 35.17894794943975 37.0 35.0 37.0 32.0 37.0 7 35.07887619363029 37.0 35.0 37.0 32.0 37.0 8 35.025528509135064 37.0 35.0 37.0 32.0 37.0 9 36.544626593806925 39.0 35.0 39.0 32.0 39.0 10-11 36.478404261191145 39.0 35.0 39.0 32.0 39.0 12-13 36.4486531986532 39.0 35.0 39.0 32.0 39.0 14-15 37.486159408290554 39.0 36.0 41.0 32.0 41.0 16-17 37.51292984489706 39.0 36.0 41.0 32.0 41.0 18-19 37.5206021968317 39.0 36.0 41.0 32.0 41.0 20-21 37.48316498316498 39.0 36.0 41.0 32.0 41.0 22-23 37.37634542142739 39.0 36.0 41.0 31.5 41.0 24-25 37.19208478224871 39.0 36.0 41.0 31.0 41.0 26-27 36.934067450460894 39.0 36.0 41.0 31.0 41.0 28-29 36.76012860848927 39.0 36.0 41.0 30.0 41.0 30-31 36.50765855274052 39.0 35.0 41.0 30.0 41.0 32-33 36.25169730087763 39.0 35.0 41.0 30.0 41.0 34-35 36.02646685433571 39.0 35.0 41.0 30.0 41.0 36-37 35.775901087376496 38.0 35.0 40.0 28.0 41.0 38-39 35.63185129988409 38.0 35.0 40.0 27.5 41.0 40-41 35.449343158359554 38.0 35.0 40.0 27.0 41.0 42-43 35.30203676105315 38.0 34.5 40.0 26.5 41.0 44-45 35.04586852127836 38.0 34.0 40.0 25.5 41.0 46-47 34.74090633107026 38.0 34.0 40.0 24.0 41.0 48-49 34.525252525252526 38.0 34.0 40.0 24.0 41.0 50-51 34.356778164155216 38.0 33.0 40.0 23.0 41.0 52-53 34.18431583595518 38.0 33.0 40.0 23.0 41.0 54-55 33.96947618259094 38.0 33.0 40.0 23.0 41.0 56-57 33.70337252304465 37.0 33.0 40.0 22.0 41.0 58-59 33.47852845393829 37.0 33.0 40.0 20.5 41.0 60-61 33.105274051995366 36.5 32.5 40.0 20.0 41.0 62-63 32.82273555224375 36.0 32.0 40.0 18.5 41.0 64-65 32.54861456090964 36.0 32.0 39.0 18.0 41.0 66-67 32.2435281779544 35.0 31.5 39.0 15.0 41.0 68-69 31.923759452447978 35.0 31.0 39.0 13.5 40.5 70-71 31.592633990174974 35.0 31.0 38.0 11.0 40.0 72-73 31.268021747529943 35.0 31.0 37.0 10.5 40.0 74-75 30.985911022796266 35.0 31.0 37.0 8.0 39.0 76-77 30.67333167743004 35.0 30.5 36.0 7.0 39.0 78-79 30.388074736435392 35.0 30.0 36.0 6.0 39.0 80-81 30.146768228735443 34.0 30.0 35.5 2.0 37.5 82-83 29.907876580007727 34.0 30.0 35.0 2.0 37.0 84-85 29.716536954241874 34.0 30.0 35.0 2.0 36.5 86-87 29.503560192084784 34.0 30.0 35.0 2.0 36.0 88-89 29.358806645691892 34.0 30.0 35.0 2.0 36.0 90-91 29.14177292046144 34.0 30.0 35.0 2.0 36.0 92-93 28.926740078379424 34.0 30.0 35.0 2.0 35.0 94-95 28.696969696969695 34.0 29.5 35.0 2.0 35.0 96-97 28.478224871667493 34.0 29.0 35.0 2.0 35.0 98-99 28.288002980625933 34.0 29.0 35.0 2.0 35.0 100 28.127587348898825 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.074046456383563 1101 2 0.0533909209440111 1101 3 -0.008549952203694033 1101 4 0.07383960110406917 1101 5 -0.13938550453289622 1101 6 0.21010123888652998 1101 7 -0.14120973952393712 1101 8 -0.05701375755327831 1101 9 0.2328525675689832 1101 10-11 0.6549522353213533 1101 12-13 0.004290030240134968 1101 14-15 0.2499843828616619 1101 16-17 0.376687623985255 1101 18-19 0.7624812981600684 1101 20-21 1.1347227567853722 1101 22-23 1.0610404621560932 1101 24-25 0.9592950690252877 1101 26-27 0.6471423959549014 1101 28-29 0.9346121067775712 1101 30-31 0.4332072645569127 1101 32-33 1.102109074354388 1101 34-35 1.2851081716028574 1101 36-37 1.084081093787887 1101 38-39 0.7050740130648734 1101 40-41 0.8333886822128633 1101 42-43 1.0965992903447912 1101 44-45 1.75808699657523 1101 46-47 1.4100764471620906 1101 48-49 0.9599851319198223 1101 50-51 -0.053485731429226746 1101 52-53 0.5022237076897582 1101 54-55 0.66420669571459 1101 56-57 1.0716496349599822 1101 58-59 0.34048325574774907 1101 60-61 0.40457991052063136 1101 62-63 0.5019460982291832 1101 64-65 1.4326834208694734 1101 66-67 1.023255298204326 1101 68-69 0.8184161536098173 1101 70-71 0.15111778159522515 1101 72-73 0.3649069889973937 1101 74-75 -0.2643490739532659 1101 76-77 0.05697788910694257 1101 78-79 -0.30974180716398436 1101 80-81 -0.265158975832648 1101 82-83 -0.1639581536565231 1101 84-85 -0.4961866636167862 1101 86-87 -0.8855499692874247 1101 88-89 0.12107758635271537 1101 90-91 0.019282519430110057 1101 92-93 0.28650771726241686 1101 94-95 0.07930837639365507 1101 96-97 -0.3248510623485288 1101 98-99 0.6879469699385545 1101 100 0.3886288885726543 1102 1 -0.5749538973998973 1102 2 -0.6429188189228974 1102 3 -0.1552529165110137 1102 4 -0.20837455497579072 1102 5 0.20423216031888813 1102 6 0.04918169865663913 1102 7 -0.19142147576108215 1102 8 0.09906609725616278 1102 9 0.16812177507049597 1102 10-11 0.6183521143292197 1102 12-13 0.4634551845051149 1102 14-15 0.6007103356747336 1102 16-17 0.46501188290842776 1102 18-19 -0.05996274297726245 1102 20-21 -0.400667442851649 1102 22-23 -0.4270115644621697 1102 24-25 -1.3011405026625553 1102 26-27 -0.7935714576446173 1102 28-29 -0.8191688974571534 1102 30-31 -0.5575670851103638 1102 32-33 0.26000381119649774 1102 34-35 -0.03839455072019149 1102 36-37 -0.39640892436092656 1102 38-39 -0.18920910853221784 1102 40-41 -0.3361515476721948 1102 42-43 -0.12693368001213656 1102 44-45 -0.2736734389964539 1102 46-47 -0.17023208278345692 1102 48-49 -1.1259192842931256 1102 50-51 -0.6186702444358829 1102 52-53 -0.7146546952140582 1102 54-55 -0.25125004959696895 1102 56-57 -0.37269398270486676 1102 58-59 -1.4142051895033134 1102 60-61 -0.8463275304957065 1102 62-63 -0.9758215968706416 1102 64-65 -0.35890762571249724 1102 66-67 -0.8258070127454644 1102 68-69 -0.10989600609979888 1102 70-71 0.23172879187955786 1102 72-73 -0.8350325149348485 1102 74-75 -0.21368361720795548 1102 76-77 -0.4102029941356484 1102 78-79 -0.1274974030502527 1102 80-81 -0.448310820962714 1102 82-83 -0.3012842274617249 1102 84-85 0.02922168484056087 1102 86-87 -0.057812522221482254 1102 88-89 -0.1776217481905391 1102 90-91 -0.7040992107574269 1102 92-93 -0.6976120649517412 1102 94-95 -0.620782367707978 1102 96-97 -1.3750627985856703 1102 98-99 -0.5740010034431755 1102 100 -0.47948968372014633 1103 1 -0.25571029339544893 1103 2 -0.2680578623044312 1103 3 0.20848456958464823 1103 4 -0.1494201612049295 1103 5 -0.04827062054874176 1103 6 0.296122744262874 1103 7 0.31747730065715984 1103 8 0.25368233752312364 1103 9 0.18814402201154223 1103 10-11 0.1611774758420026 1103 12-13 -0.30767940948820893 1103 14-15 -0.40791035398044073 1103 16-17 -0.09727955201926619 1103 18-19 0.7300817509048301 1103 20-21 0.11434924235413035 1103 22-23 -0.21371901718742237 1103 24-25 -0.16209712112751617 1103 26-27 -0.6561965966879981 1103 28-29 -0.8653313001267833 1103 30-31 -0.49944695162871255 1103 32-33 -0.28838328557769444 1103 34-35 -1.203856174746882 1103 36-37 -2.244017378198528 1103 38-39 -2.2843147815021894 1103 40-41 -1.7822027156366005 1103 42-43 -1.7554097645505138 1103 44-45 -2.1888569762600767 1103 46-47 -2.8123344187122683 1103 48-49 -2.111180685850414 1103 50-51 -2.237413292266808 1103 52-53 -1.9718849510538803 1103 54-55 -2.141961951710428 1103 56-57 -1.7809253508917422 1103 58-59 -2.116930439781399 1103 60-61 -2.1399871522977065 1103 62-63 -2.14406408852804 1103 64-65 -1.5279843776591022 1103 66-67 -1.5771974465608096 1103 68-69 -1.580140722451297 1103 70-71 -1.810540396107097 1103 72-73 -1.3283584326211653 1103 74-75 -1.0596235504671334 1103 76-77 -0.88628753251162 1103 78-79 -0.8444050782449075 1103 80-81 -1.2992563159571517 1103 82-83 -0.8949994666163406 1103 84-85 -0.42572825954208326 1103 86-87 -0.6348425555918951 1103 88-89 -0.7599354300592793 1103 90-91 -0.29976105725353364 1103 92-93 0.07046357464781394 1103 94-95 -0.20337413633979295 1103 96-97 -0.5355188608771044 1103 98-99 -0.34374516362114704 1103 100 -0.6557966915065769 1104 1 -0.18728409593699524 1104 2 0.39469873593603566 1104 3 -0.12338248808886121 1104 4 -0.09761175455781768 1104 5 0.0025602689918216015 1104 6 -0.0020199732346810606 1104 7 0.461661073873195 1104 8 0.02592085169712277 1104 9 -0.00638188219178204 1104 10-11 0.44881188444416154 1104 12-13 0.06011140185098185 1104 14-15 0.5996654035953171 1104 16-17 0.6207067627412428 1104 18-19 0.5607269121951504 1104 20-21 0.8093639051107289 1104 22-23 -0.12476496049142582 1104 24-25 0.18736524446387648 1104 26-27 -0.4748671734230925 1104 28-29 0.09410173995141236 1104 30-31 -0.31200804644787183 1104 32-33 -0.6112083100156269 1104 34-35 -0.871989117073376 1104 36-37 -0.17827935278307905 1104 38-39 0.5411984149787514 1104 40-41 -0.3938840450598633 1104 42-43 0.18471731267333524 1104 44-45 -0.2582606908250824 1104 46-47 0.6461211042593789 1104 48-49 0.6441956582356099 1104 50-51 0.4536913020635964 1104 52-53 -0.08429941830705445 1104 54-55 0.5146851646141073 1104 56-57 1.2542652649440313 1104 58-59 1.0837049463378605 1104 60-61 0.9125543921793238 1104 62-63 0.32172281274273473 1104 64-65 0.43706938578175425 1104 66-67 0.050368535843880125 1104 68-69 0.2869169510579894 1104 70-71 0.8038291053591813 1104 72-73 0.018415760927219083 1104 74-75 0.22727772030510707 1104 76-77 0.3468502974003975 1104 78-79 0.9422518132506887 1104 80-81 1.3317309763204612 1104 82-83 1.6287053232174813 1104 84-85 1.2350733044852866 1104 86-87 0.8807977181128948 1104 88-89 0.9556070919348514 1104 90-91 0.8737482291589806 1104 92-93 0.9651361064171198 1104 94-95 0.6710149154686142 1104 96-97 -0.13864691561806097 1104 98-99 -0.05806578923209926 1104 100 0.56805472589323 1105 1 0.8077481941772646 1105 2 0.6838194512862579 1105 3 0.6302191015783052 1105 4 0.09886117142330875 1105 5 0.6265046306411008 1105 6 0.778407249815487 1105 7 1.0120833292134819 1105 8 1.2251260181154038 1105 9 1.2489584059353902 1105 10-11 1.040341939088968 1105 12-13 0.857180453018394 1105 14-15 0.6904503017572665 1105 16-17 0.7453962808733792 1105 18-19 1.0853170761295772 1105 20-21 1.344386259805212 1105 22-23 1.6474655412472714 1105 24-25 1.1409171570321917 1105 26-27 1.4955749527567406 1105 28-29 1.2808876316553395 1105 30-31 1.8535408854945032 1105 32-33 1.5796759423440392 1105 34-35 2.407196178505359 1105 36-37 1.6582829919759803 1105 38-39 2.090751321089037 1105 40-41 2.082022561994279 1105 42-43 1.8448017637414011 1105 44-45 2.227457143253403 1105 46-47 2.735788267697032 1105 48-49 2.428348663700092 1105 50-51 2.960894481397922 1105 52-53 3.0626206015637862 1105 54-55 2.7644367791198263 1105 56-57 2.201359154660871 1105 58-59 2.0439632672519252 1105 60-61 2.155227020097854 1105 62-63 1.619288634898723 1105 64-65 2.0941732109183633 1105 66-67 2.384198640165792 1105 68-69 1.984507611763405 1105 70-71 2.12983506661104 1105 72-73 2.637268219943614 1105 74-75 2.231624466455134 1105 76-77 2.4113063331679108 1105 78-79 1.4243680576620186 1105 80-81 1.349738924656279 1105 82-83 1.2727689099040198 1105 84-85 2.067661481335758 1105 86-87 1.8445334359469712 1105 88-89 1.7309424123521424 1105 90-91 2.1696995456020964 1105 92-93 2.5518226439233302 1105 94-95 2.752733743089081 1105 96-97 2.4595895473724987 1105 98-99 3.134308776093288 1105 100 2.431194318541017 1106 1 0.7716270494148283 1106 2 0.4809853306972087 1106 3 -0.07664391592656017 1106 4 0.4511812603368952 1106 5 0.12942210771807083 1106 6 -0.24187616224341468 1106 7 0.3024417621115134 1106 8 0.18205849936019547 1106 9 -0.19949949903821107 1106 10-11 0.2948570821842722 1106 12-13 0.7036655877780547 1106 14-15 0.490394730765324 1106 16-17 -0.11914942626749081 1106 18-19 0.4251336918561677 1106 20-21 0.44649795749901244 1106 22-23 0.9065652911778059 1106 24-25 0.5828454704525825 1106 26-27 0.21285753125076212 1106 28-29 0.10514436963811136 1106 30-31 0.2903288821602459 1106 32-33 0.2515292349253144 1106 34-35 0.2168655311033021 1106 36-37 0.5631176682749555 1106 38-39 0.44682245195872383 1106 40-41 -0.0931649901035243 1106 42-43 0.3943988740498199 1106 44-45 0.2210664791800525 1106 46-47 0.7606562865911712 1106 48-49 0.6215967881791187 1106 50-51 0.9708972599475203 1106 52-53 1.0503949833364956 1106 54-55 0.7711052981528823 1106 56-57 1.130228284974848 1106 58-59 1.5600788549151616 1106 60-61 1.462117823098687 1106 62-63 1.4100638502363125 1106 64-65 1.4695552614879688 1106 66-67 1.760692110574233 1106 68-69 2.0780058057061623 1106 70-71 1.6485902763915412 1106 72-73 2.081975082961115 1106 74-75 1.1403491121900586 1106 76-77 1.4350629322232038 1106 78-79 1.9129760043138653 1106 80-81 1.7052543456065443 1106 82-83 1.4558753283535886 1106 84-85 0.646989072333259 1106 86-87 1.385933824943919 1106 88-89 1.1948212675897096 1106 90-91 1.1902608126207141 1106 92-93 1.6058654335871267 1106 94-95 1.5418418286684101 1106 96-97 1.2289699308123403 1106 98-99 1.7915746832897312 1106 100 2.020802543047825 1107 1 -0.2563076649605627 1107 2 0.07483341607071736 1107 3 -0.20250706721344258 1107 4 -0.05384071078677266 1107 5 0.4015501679817248 1107 6 0.19158272036801094 1107 7 0.33539844761056514 1107 8 0.21784004361631304 1107 9 0.4969851211752214 1107 10-11 0.28214521777749013 1107 12-13 0.5525356442752312 1107 14-15 0.5087563126862307 1107 16-17 0.3606057526402324 1107 18-19 0.375541727009967 1107 20-21 0.3506096963565213 1107 22-23 0.8558747701482972 1107 24-25 0.909587466686105 1107 26-27 0.5672203686644508 1107 28-29 0.5208694167190941 1107 30-31 0.26459128015145694 1107 32-33 0.2877815889742692 1107 34-35 -0.5521237972080613 1107 36-37 -0.2380436625473905 1107 38-39 0.3366529604332982 1107 40-41 0.6204257192498943 1107 42-43 0.40020552816155686 1107 44-45 0.4715372889728968 1107 46-47 0.9188483769866522 1107 48-49 1.4775289915689456 1107 50-51 1.4991458475181432 1107 52-53 0.4510541170464819 1107 54-55 1.4010488009777404 1107 56-57 1.6052753659541352 1107 58-59 1.4018867645196806 1107 60-61 1.842689072314002 1107 62-63 1.5969753379952607 1107 64-65 1.8166990154113805 1107 66-67 0.7776412631166068 1107 68-69 -0.1903557762147372 1107 70-71 0.6506304521605308 1107 72-73 0.8424898350012953 1107 74-75 0.8409140839414668 1107 76-77 0.7104866610367644 1107 78-79 0.3808040018028791 1107 80-81 0.7046265992160841 1107 82-83 0.7785369730491318 1107 84-85 0.9497197691317503 1107 86-87 1.1139627012778774 1107 88-89 -0.13866900234123847 1107 90-91 0.36869772410847546 1107 92-93 0.6427455340265453 1107 94-95 0.566637811091514 1107 96-97 0.9295349025637556 1107 98-99 1.2820015508352434 1107 100 0.593307251145756 1108 1 -0.32339934680634386 1108 2 -0.29978710609078973 1108 3 -0.10748581645448496 1108 4 0.04968084355445512 1108 5 0.5445374175263495 1108 6 -0.1560189796927105 1108 7 -0.05349044127832059 1108 8 0.15955224762360132 1108 9 0.281745291181295 1108 10-11 0.38460423417092926 1108 12-13 0.14406569892003773 1108 14-15 0.0019257115933299929 1108 16-17 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2215 70-71 -2.418124472977375 2215 72-73 -2.2847757284344823 2215 74-75 -2.4321355614486 2215 76-77 -3.8161090836440863 2215 78-79 -2.94169402814196 2215 80-81 -2.781583014653105 2215 82-83 -2.990572562017853 2215 84-85 -2.72463011266494 2215 86-87 -2.75975676286712 2215 88-89 -2.629015732049673 2215 90-91 -2.192176980383252 2215 92-93 -3.3988226298820408 2215 94-95 -3.366380313415977 2215 96-97 -3.8222381470816345 2215 98-99 -3.6858865500315794 2215 100 -2.962248304409801 2216 1 -1.1523190609719585 2216 2 -0.5120611708238698 2216 3 -1.3121936769000513 2216 4 -0.756119913065973 2216 5 -0.49161222518067405 2216 6 -0.6104115816262947 2216 7 -0.9765673643552404 2216 8 -0.6966898709136018 2216 9 -1.3676872434971443 2216 10-11 -0.9422137565814808 2216 12-13 -0.735925894186316 2216 14-15 -1.4591924052624918 2216 16-17 -1.8338386918042104 2216 18-19 -1.8751705237022804 2216 20-21 -2.148678052931224 2216 22-23 -1.7075155432420175 2216 24-25 -1.1203270632284301 2216 26-27 -2.273235471791395 2216 28-29 -1.4641733183236454 2216 30-31 -2.0701665546063808 2216 32-33 -2.7015346503419693 2216 34-35 -3.2344599795442406 2216 36-37 -1.9638271383308705 2216 38-39 -2.740270116489782 2216 40-41 -3.371739522915341 2216 42-43 -3.381133503177473 2216 44-45 -3.3532490358134304 2216 46-47 -3.734997776859501 2216 48-49 -2.1634966494566896 2216 50-51 -2.103418255045966 2216 52-53 -3.217749301756939 2216 54-55 -3.1758742759453327 2216 56-57 -3.5592545485757796 2216 58-59 -2.5340106713777573 2216 60-61 -2.8660003863754575 2216 62-63 -3.041913550893632 2216 64-65 -3.97959728088491 2216 66-67 -2.7864613009859553 2216 68-69 -3.2672788531378174 2216 70-71 -2.8144692629391876 2216 72-73 -2.334148341636883 2216 74-75 -2.9154961724687887 2216 76-77 -2.3600261594760568 2216 78-79 -2.072899701900827 2216 80-81 -2.5674531728636865 2216 82-83 -1.9750242805121232 2216 84-85 -2.6635280941161135 2216 86-87 -2.351137013821834 2216 88-89 -2.4086120222842595 2216 90-91 -2.154806549395797 2216 92-93 -1.279619138338127 2216 94-95 -1.4105701661164645 2216 96-97 -3.121747148718299 2216 98-99 -2.9024713836376925 2216 100 -3.68019702235852 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 1.0 5 8.0 6 22.0 7 73.0 8 116.0 9 131.0 10 145.0 11 206.0 12 211.0 13 244.0 14 215.0 15 251.0 16 276.0 17 335.0 18 326.0 19 227.0 20 196.0 21 234.0 22 243.0 23 273.0 24 319.0 25 392.0 26 438.0 27 525.0 28 641.0 29 829.0 30 1012.0 31 1189.0 32 1504.0 33 1761.0 34 2314.0 35 3034.0 36 4374.0 37 6183.0 38 6139.0 39 1838.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.54224185532855 20.83655438983987 13.517393705135284 27.103810049696303 2 13.48181266214053 21.780648010156206 37.02599768173538 27.711541645967873 3 14.836893525418116 27.399679858696253 30.413423856046805 27.350002759838826 4 10.697224906806573 18.931382024023193 35.22297390584012 35.14841916333011 5 12.49137526564182 36.825545773190186 34.08219026853973 16.600888692628267 6 28.804437820831264 37.08947397471988 18.731026108075287 15.37506209637357 7 25.641662526908426 32.51366120218579 22.161505768063144 19.683170502842636 8 25.492631230336148 34.03157255616272 21.02721201081857 19.448584202682564 9 24.788872329855938 16.92609151625545 21.824805431362808 36.46023072252581 10-11 23.460981163320223 26.665286690126266 28.17084109570137 21.702891050852134 12-13 24.917204835237623 24.36937682839322 28.184854004526134 22.52856433184302 14-15 22.64723740133576 26.008721090688304 26.68074184467627 24.66329966329966 16-17 21.13760556383507 29.359165424739196 26.825633383010434 22.6775956284153 18-19 21.090688303803056 26.861511287740797 29.822818347408514 22.224982061047633 20-21 22.231881658111167 26.154992548435168 30.314069658331956 21.29905613512171 22-23 22.095269636253242 26.432356350389135 30.15951868410885 21.312855329248773 24-25 21.833386230974096 26.42996122372942 30.12129659017208 21.6153559551244 26-27 21.554341226472374 26.850471932439145 30.105701827013302 21.48948501407518 28-29 21.17900314621626 26.470994093944917 30.503118617872715 21.846884141966108 30-31 21.664251707720968 27.16483819775064 29.43075967708549 21.7401504174429 32-33 21.136225644422364 26.342661588563228 30.17469779764862 22.34641496936579 34-35 21.538376618000164 26.217812491375263 30.328705875859026 21.915105014765544 36-37 22.4384185468847 26.507969364520807 28.794590491961635 22.25902159663286 38-39 21.763009369782107 26.54173623856376 29.471345577987222 22.22390881366691 40-41 21.807167400333945 25.858666703465026 29.532062869996 22.80210302620503 42-43 21.625015524307617 26.285067685981208 29.7073150537486 22.382601735962577 44-45 21.766848816029142 26.549649500469176 29.422641717723685 22.260859965778 46-47 22.21532262515869 26.451675222167026 28.66782579897334 22.665176353700943 48-49 22.191863995142683 25.978362863608766 28.78787878787879 23.041894353369763 50-51 21.90760059612519 26.31644311972181 28.47739692001987 23.298559364133133 52-53 22.207043108682452 26.559308936358118 28.351824253463597 22.881823701495833 54-55 22.09971435273987 26.986076420991623 27.92443457021817 22.98977465605034 56-57 21.755809460727495 26.846332174201027 28.07446045150963 23.32339791356185 58-59 21.589435045400602 26.622140038086826 28.602379046725364 23.18604586978721 60-61 21.863443174918583 27.239609206822323 27.856433184302038 23.040514433957057 62-63 21.71717171717172 27.10851686261522 27.97096649555666 23.2033449246564 64-65 21.69371308715571 26.981564276646242 28.042722305017385 23.282000331180658 66-67 21.689573328917593 26.951206049566707 27.929568913175473 23.42965170834023 68-69 22.266379643428824 26.724899265882872 27.842633990174974 23.16608710051333 70-71 22.3477948887785 26.339901749737816 27.815035601920847 23.497267759562842 72-73 22.449908925318763 26.30402384500745 28.020643594414086 23.225423635259702 74-75 21.913120273776013 26.3799194127063 28.124137550367056 23.58282276315063 76-77 22.16288568747585 26.28608489264227 27.726720759507646 23.824308660374236 78-79 22.367113760556386 26.56482861400894 27.6949826130154 23.373075012419275 80-81 22.398851907048627 26.563448694596236 27.930948832588175 23.10675056576696 82-83 22.058011812110173 26.773196445327592 27.722581001269525 23.44621074129271 84-85 21.740630347187725 26.450295302754316 28.1544957774466 23.65457857261136 86-87 22.33399569465143 26.334382072086992 27.59700833471325 23.734613898548325 88-89 21.628856874758515 26.522051112215046 27.641165755919854 24.207926257106585 90-91 21.867582933156704 26.334382072086992 27.848153667825798 23.94988132693051 92-93 21.690953248330295 26.68902136115251 27.58734889882431 24.032676491692886 94-95 21.889661643760007 26.228128277308606 27.6729039024121 24.20930617651929 96-97 21.73373075012419 26.41165755919854 27.809515924270023 24.045095766407243 98-99 21.788927526632445 26.5772478887233 27.50179389523652 24.13203068940774 100 21.76408897720373 26.28194513440415 27.581829221173482 24.372136667218637 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 87.0 1 61.0 2 52.0 3 63.5 4 47.0 5 33.5 6 33.5 7 55.5 8 86.0 9 93.0 10 67.0 11 35.0 12 36.5 13 44.5 14 42.0 15 44.0 16 51.0 17 57.5 18 57.0 19 57.5 20 73.5 21 98.5 22 106.5 23 115.5 24 135.0 25 149.5 26 186.0 27 256.5 28 312.0 29 374.0 30 451.0 31 500.0 32 569.5 33 641.0 34 696.0 35 749.5 36 825.0 37 899.0 38 961.0 39 1006.5 40 1031.0 41 1072.0 42 1100.0 43 1141.0 44 1157.0 45 1150.0 46 1181.0 47 1204.0 48 1324.5 49 1340.5 50 1232.0 51 1196.5 52 1151.5 53 1119.5 54 1141.0 55 1101.5 56 958.0 57 850.5 58 787.0 59 761.5 60 733.0 61 683.0 62 580.5 63 444.5 64 361.0 65 319.0 66 262.0 67 184.5 68 139.5 69 109.0 70 66.0 71 43.5 72 37.5 73 33.0 74 25.5 75 19.5 76 11.0 77 5.0 78 4.5 79 2.5 80 2.0 81 1.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.038637743555776344 2 0.0 3 0.0 4 0.05243693768283932 5 0.0027598388254125956 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.004139758238118894 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0013799194127062978 26-27 0.0 28-29 0.0 30-31 0.004139758238118894 32-33 0.0 34-35 0.0027598388254125956 36-37 0.004139758238118894 38-39 0.0013799194127062978 40-41 0.0013799194127062978 42-43 0.0013799194127062978 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0013799194127062978 56-57 0.0 58-59 0.0027598388254125956 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 36234.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.58806645691891 #Duplication Level Percentage of deduplicated Percentage of total 1 95.6073397925169 84.69669371308716 2 2.8630175394872115 5.072583761108351 3 0.6324184554035951 1.6807418446762707 4 0.2772672045858126 0.9825026218468841 5 0.1526527306146609 0.676160512226086 6 0.09034549362908502 0.4802119556217917 7 0.07165332253341225 0.4443340508914279 8 0.05919187513629708 0.4194955014627146 9 0.04361506588990311 0.34773969200198707 >10 0.18380634910744884 3.052381740906331 >50 0.009346085547836381 0.6596014792736105 >100 0.009346085547836381 1.4875531268973892 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 269 0.7423966440359883 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 157 0.43329469558977757 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 113 0.31186178727162334 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 95 0.2621846884141966 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 86 0.23734613898548323 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58 0.16007065187393055 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 50 0.1379919412706298 No Hit CCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCT 44 0.12143290831815423 No Hit CCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCC 43 0.11867306949274162 No Hit TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG 37 0.10211403654026605 RNA PCR Primer, Index 23 (96% over 30bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.002759838825412596 0.0 30-31 0.0 0.0 0.0 0.005519677650825192 0.0 32-33 0.0 0.0 0.0 0.013799194127062979 0.0 34-35 0.0 0.0 0.0 0.03173814649224485 0.0 36-37 0.0 0.0 0.0 0.05381685709554562 0.0 38-39 0.0 0.0 0.0 0.08969476182590937 0.0 40-41 0.0 0.0 0.0 0.1269525859689794 0.0 42-43 0.0 0.0 0.0 0.16697024893746204 0.0 44-45 0.0 0.0 0.0 0.22078710603300766 0.0 46-47 0.0 0.0 0.0 0.31324170668432966 0.0 48-49 0.0 0.0 0.0 0.37119832201799413 0.0 50-51 0.0 0.0 0.0 0.4553734061930783 0.0 52-53 0.0 0.0 0.0 0.5271292156538059 0.0 54-55 0.0 0.0 0.0 0.6016448639399459 0.0 56-57 0.0 0.0 0.0 0.7285974499089254 0.0 58-59 0.0 0.0 0.0 0.8417508417508417 0.0 60-61 0.0 0.0 0.0 0.9700833471325274 0.0 62-63 0.0 0.0 0.0 1.088756416625269 0.0 64-65 0.0 0.0 0.0 1.2377877131975492 0.0 66-67 0.0 0.0 0.0 1.3550808632775846 0.0 68-69 0.0 0.0 0.0 1.505492079262571 0.0 70-71 0.0 0.0 0.0 1.6572832146602638 0.0 72-73 0.0 0.0 0.0 1.83391289948667 0.0 74-75 0.0 0.0 0.0 2.017442181376608 0.0 76-77 0.0 0.0 0.0 2.231329690346084 0.0 78-79 0.0 0.0 0.0 2.478335265220511 0.0 80-81 0.0 0.0 0.0 2.7570789865871834 0.0 82-83 0.0 0.0 0.0 3.060661257382569 0.0 84-85 0.0 0.0 0.0 3.30628691284429 0.0 86-87 0.0 0.0 0.0 3.589170392449081 0.0 88 0.0 0.0 0.0 3.792018546116907 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACCCA 20 0.0021460487 70.49999 4 GGTATCA 55 1.240187E-8 59.81818 1 GGCATTG 25 0.0051952875 56.399998 8 GTATCAA 90 1.2543751E-8 41.777775 1 TATCAAC 155 0.0 39.419357 1 CATGGGA 60 8.655844E-5 39.166668 4 GTACATG 195 0.0 36.153847 1 ATCAACG 170 0.0 35.941177 2 CATGGGT 55 0.0026825548 34.181816 4 TACATGG 210 0.0 33.57143 2 ACATGGG 210 0.0 33.57143 3 TCAACGC 185 0.0 33.027027 3 CAACGCA 200 0.0 30.55 4 AACGCAG 205 1.8189894E-12 29.804878 5 ACGCAGA 225 3.6379788E-12 27.155556 6 CGCAGAG 225 3.6379788E-12 27.155556 7 CATGGGG 105 7.228351E-5 26.857143 4 GCAGAGT 245 3.074092E-10 23.020409 8 CAGAGTA 270 1.038643E-9 20.88889 9 AGAGTAC 270 0.0 17.407408 10-11 >>END_MODULE